Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F9 I0 R1	86	138.2	2894862	96.7%	2799331	224.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,206,477	T→A	19.3%	S5R (AGT→AGA)	ttdA →	L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,477	0	T	A	19.3%	170.3 / 14.9	111	S5R (AGT→AGA)	ttdA	L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand): ref base T (25/59); new base A (11/10); total (42/69)
Fisher's exact test for biased strand distribution p-value = 7.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCG  >  NC_000913/3206249‑3206712

ttCAGCATCGGTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAGT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAc                                                                                                                                                                                                                                                 <  2:927167/239‑1 (MQ=255)

      aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑Ag‑‑‑‑‑‑g                                                                                                                                                                                                                                       <  1:588961/236‑1 (MQ=255)

      aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑Ag‑‑‑‑‑‑g                                                                                                                                                                                                                                       <  2:597756/236‑1 (MQ=255)

       tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATAAAAATAC‑AAAAAAAGGAGTTTGCAT‑GATGAGGGAAAGAAATAAGAAAA‑Agtctct                                                                                                                                                                                                                                         >  2:288314/1‑234 (MQ=255)

       tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAA‑AAAAAATTGAGTTGGCAT‑GATGAGCGGAAAGAAAAAACAAC‑AA‑‑‑‑‑‑GGA‑Gt                                                                                                                                                                                                                                  >  1:398496/1‑239 (MQ=255)

        cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑Gc                                                                                                                                                                                                                                      <  1:844009/235‑1 (MQ=255)

        cGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTg                                                                                                                                                                                                                                 <  1:1290429/239‑1 (MQ=255)

          ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTTGGATTGACAC‑TAGGAGGGAAAGTAATAAAAAAC‑AA‑‑‑‑‑‑GAA‑ATGaa                                                                                                                                                                                                                               >  2:601997/1‑239 (MQ=255)

            aaTTATCCGCAGGTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATa                                                                                                                                                                                                                             <  2:1382686/239‑1 (MQ=255)

                aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAc                                                                                                                                                                                                                                                 <  1:1084271/223‑1 (MQ=255)

                 tCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTg                                                                                                                                                                                                                        <  1:1083770/239‑1 (MQ=255)

                                aGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAATAA‑AAAAAAATGAGTTGTCATGGTTGAG‑GGAAGTAAAAAACAAC‑AA‑‑‑‑‑‑GCA‑GGGAAAAAAGTG‑ACAGAGATGTTCGc                                                                                                                                                                                                         >  2:894581/1‑238 (MQ=255)

                                aGCGAAGGGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCGGGCGCAATATTCATTCTTATTTCCGCGTTGATTCACGAACTGTTAATTACTTATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCt                                                                                                                                                                                                        <  2:800534/239‑1 (MQ=255)

                                  cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCACAAAAAAAC‑AAAAAACAGAGTTTGCAT‑CTGTGGCGAAAGTAATAAACAAA‑AA‑‑‑‑‑‑GCACGTAACTAA‑TTG‑GCAGAGAtttagcgat                                                                                                                                                                                                       >  2:147067/1‑231 (MQ=255)

                                  cgcAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCTACAAATTC‑AAAAAATTGAGTTTCACT‑GATGAGCGCAAATAAAAAACACA‑AG‑‑‑‑‑‑GCA‑GGGAATAAAATT‑ACATAGATTGTTTCTaa                                                                                                                                                                                                      >  1:800534/1‑239 (MQ=255)

                                       tgtTTTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTa                                                                                                                                                                                                 <  1:1082447/239‑1 (MQ=255)

                                              cGCAAAACATTCCCA‑TGCCTTGCTTCGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCAAACTTTTAATTACATATA‑CTCGGTGGCAAAAAATAAAAACCAAAATTTTTGGTGGTAGTTTGGGGAGGTGAATTTTTCCAGAAAAAAAC‑AAAAACTGAGGTTGCAAT‑GATGAGCGAAAGTAAAAAGCACC‑AG‑‑‑‑‑‑GAA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAACTTTACCGCCAt                                                                                                                                                                                          <  1:618457/239‑1 (MQ=255)

                                                 aaaaatttGCCATTTCCCTG‑TCTGGCGACATTTTATTTTTTTTTTTCGGGTTGATTCACAAACTTGTTATTAAATATTACAGGGTGAAAAAAAATA‑AAAACCATATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAACAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTTA‑ACAGAGTTTGTCGTTAACTTTACCGCCATG‑At                                                                                                                                                                                      <  2:1306196/233‑1 (MQ=255)

                                                 aaaaCATTGCCA‑TTCCCTGTTCTGGCGAAATATTCATTCTTATTTTCGCGTTGATTCCCGAATTGTTAATTACATATT‑CACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGTTAACTTTACCGCCATG‑At                                                                                                                                                                                      <  1:1220250/239‑1 (MQ=255)

                                                 aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATAA‑AAAAAATGGAGTTGGCAT‑GATGAGCGAAAGGAATAAGAAAA‑AA‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAAAGAATGTCGCTAACTTTAACGCCATG‑At                                                                                                                                                                                      >  1:251319/1‑239 (MQ=255)

                                                 aaaaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAAAGGAGGTGCCAT‑GATGAGCGGAAGAAATAAGCAAA‑AA‑‑‑‑‑‑GCA‑GTGAATAAGTTT‑ACAGAGATTGTGGCTAAATTTATCCCCCTG‑At                                                                                                                                                                                      >  1:59610/1‑239 (MQ=255)

                                                   aaCATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CCCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑Attt                                                                                                                                                                                    <  1:1192224/239‑1 (MQ=255)

                                                        tGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCAGAAAAAAAA‑AAAAAAATGAGTTGTCAT‑TATTAGCGAAAATAATAAACAAA‑AA‑‑‑‑‑‑GCA‑GGGGAAAAAATT‑AAAGAGATTGGTGCTCACTTTTACGCCATG‑Aatttttc                                                                                                                                                                                >  1:642912/1‑234 (MQ=255)

                                                          ccA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAC‑AAAAAATTGAGTTGTCAT‑GATGAGGGAAAGGAATAAGAAACAAGCAAAAAGCA‑GCGGATAAGTTG‑ACAGAGATTTTTGCTAAATTTACCCACATG‑Attt                                                                                                                                                                                    >  1:558935/1‑239 (MQ=255)

                                                            ctttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGACAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GCGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGa                                                                                                                                                                            <  1:677346/237‑1 (MQ=255)

                                                            a‑TTCCCTGCTCTGGCACAATATTCTTTCTTTTTTTCGCGTTGATTCACGAACGGTTAATTACATTTT‑CACGGTGGCAAAAAATATAAAACCAAATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGaa                                                                                                                                                                           <  1:482289/239‑1 (MQ=255)

                                                                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAA‑AAAAACTTGAGTTTGCCT‑TTTGAGGGAAAGTAATAAAAAAA‑AA‑‑‑‑‑‑GCA‑ACGAATAAGATT‑AGAGAGAATGTGGGTCACTTTACCGCCCTG‑ATTTCTATCCGAATGc                                                                                                                                                                        >  2:970454/1‑239 (MQ=255)

                                                                         ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAATCGAGTTTTCCT‑GATGTGGGAAAGTAATAAACAAC‑AA‑‑‑‑‑‑GCA‑ATGAATAAATTT‑ACAGAGAATGTCGCTAAATTTAACTCCATG‑TTTTTTACCCGCATGACcgatgtgg                                                                                                                                                               >  1:623602/1‑236 (MQ=255)

                                                                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCAAAAAAAAACAAAAAACTGCGGTTGCC‑T‑GATGAGGGGAAGCAATTAACAAC‑AC‑‑‑‑‑‑GCA‑CAGAATAAGTTT‑TCAGAGATTGTCGTTACCTTTATAGCCCCG‑CTTTCTATCCGAATGccccgggacgt                                                                                                                                                              >  2:968220/1‑230 (MQ=255)

                                                                           cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAA‑AAAAAATGGAGTTGCCAT‑GATGAGCGAAAGAAAAAAAAAAC‑AA‑‑‑‑‑‑GCA‑GAGAATAAGTTG‑ACAGAGATTGGCGCTAACTTTTCCGCCATG‑ATTTTTACCCCAATGCCtgattaaggg                                                                                                                                                             >  1:1349641/1‑233 (MQ=255)

                                                                            gcAATATTCATTCTTATTTGCGCGTTGATTCACGAACTGTTAATTACATATT‑CAGGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAAACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAAAAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACgtg                                                                                                                                                             <  1:970454/238‑1 (MQ=255)

                                                                                tatTCTATCTTATTTTCGAGTTTATTCACGAACTGTTAATTAAATATC‑CAGGGTGCCAAAAAATATAAAACCTCATTTGTAGGGGAAGTTTGTGGGGGTGAATTTTCCAAGACAAATAC‑AAAACCTGGAGTTGCCAT‑GAGTAGCAAAAATAAAAAGCACC‑AG‑‑‑‑‑‑GCA‑GTGAATAATTTG‑ACAGAATTTGCCGCTATCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                        <  2:1286999/239‑1 (MQ=255)

                                                                                aactaattcTTATTTTCGCTTTGATACATGAACTGTAAATTCCATATA‑CACGGTGGAAAAAAATAAAAAACAAATTTTTTGGGGTTATTTTATGGGGGAGAATTGTTCAAAAAAAATAC‑AAAATCGGGAGTGGCAGT‑AAGGAAAAAAAGTAAGAAGCAGA‑AG‑‑‑‑‑‑ACA‑TGAAATAAGTGG‑AAGGGAATTGGCGCAAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                        <  2:1291506/234‑1 (MQ=255)

                                                                                  ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAACTGGGGTTTCCAT‑GATGAGCGAGAGAAATAAGCAAC‑AA‑‑‑‑‑‑GCA‑GTGAATAAATTT‑ACAGAGATTGTCGCTAAATTTACCGCCATG‑ATTTCTACCCCCATGCCTGTTGGCGGGGGGGCTa                                                                                                                                                      >  1:1245946/1‑239 (MQ=255)

                                                                                        tttATTTTAGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGAAAAAAAAATAAAACCACTTTTTAAGTGTGAGTTTGTGGCGTGGAATTTTTCAAGAAAAAAAC‑AAAAACTGGAGTTGCCAT‑GTGAAGAAAAATTAATAAGCACC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCCCTAACTTTTCCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                                               <  1:968220/238‑1 (MQ=255)

                                                                                        cTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑aa                                                                                                                                               <  1:328413/239‑1 (MQ=255)

                                                                                         ttATTTTCGCGTTGATTCACGAACTGTTAATTACATTGT‑CACGGTGGAAAAAAATAGAAACCCACATTTGTAGTGGTATTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGACTGATGACGTGGTGGATAAACT‑aaa                                                                                                                                              <  1:359612/239‑1 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTACACAAATAC‑AAAAAATGGAGTTTGCTT‑TATGAGCGGAAGTAATAAACAAC‑AG‑‑‑‑‑‑ACA‑GAGAAACAGATT‑AAAAAAATTGTAGGTAAATTTTAAGCCATG‑AGTTTTACCCCAATGCCTTGTTACGGGGTGGATAAACC‑aaac                                                                                                                                             >  2:958061/1‑238 (MQ=255)

                                                                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTAAGACAAAAAC‑AAAAAATAGAGTTTTCAT‑TATGAGCGGAAGAAATAAAAAAA‑AG‑‑‑‑‑‑AAA‑ATGAAAAAGATG‑GCAGAGAGTGTTGCTAACTATACCGCCCTG‑ATTTTTAACCGAATGCCTGATGAAGGGGTGGATAAACA‑aaaa                                                                                                                                             >  2:475605/1‑239 (MQ=255)

                                                                                            ttttCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTACGAAAAAAAC‑AAAAAATGGAGTTTTCAT‑TGTGGGAGGAAAGAAAAAACAAA‑AA‑‑‑‑‑‑GCA‑GAGAAAAAATTG‑ACAAAGATTGTGGCGTACTTTTTCGCCCTG‑ATTTCTTTCCGAATACCTGATGACGTGGGGGGTATACA‑aaaaaa                                                                                                                                           >  2:582594/1‑237 (MQ=255)

                                                                                                 gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCAGACAAAAAA‑AAAAACAGTAGGGTCCTG‑TATTGGGGGAAGTTATAAGCAAA‑AA‑‑‑‑‑‑GCA‑GTGAATAAAATT‑GCAGAAATTGTCGCTAACATTACCCCCCAG‑AATTCTTCCCAAATGCCTGATTACGCGGTGGATAAATT‑aaaaagagtta                                                                                                                                      >  2:1151044/1‑232 (MQ=255)

                                                                                                 gcgTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAA‑AAAAAAAGGAGTTGGCAT‑TATGAGGGGAAGTAATAAGAAAC‑AA‑‑‑‑‑‑GCA‑ATGAATAAGATG‑GCAGAGAATGTTGCTAAATTTACCACCCTG‑ATTTCTACCCGAATACATGATGACTGGGGGGGTAAACA‑aaaaaaaataa                                                                                                                                      >  2:1102120/1‑232 (MQ=255)

                                                                                                    tttaTTCAGGAATTTTTAATTAAATATT‑CAGGGTGCAAAAAAATAAAAAACCCTATTTTTGGTGGTGGTTTGTGGGGGTGAATTTTTCCAAAAAAATAA‑AAAAACGGGATTTGCCAT‑GGTGAGGGAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg                                                                                                                                    <  2:1245946/235‑1 (MQ=255)

                                                                                                     gaattaccGAACTGTTAATTACATATT‑ACGGGTGGAAAAAAATATAAAACCACATTTTTAGTGTTGTTTTGTGGAGGTGAATTTTTACAGACAAATAC‑AAAATCTGGAGTGCCCGT‑GAGGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCG‑GTGAATAAGTTG‑ACAGAGTTTTTCACTAACTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAg‑g                                                                                                                                  <  2:1280570/231‑1 (MQ=255)

                                                                                                                  gTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGAGGTGCCCT‑GATGAGGGAAAGTAATAAGCAAA‑AA‑‑‑‑‑‑GCA‑GAGAATAAGTTT‑ACAGAGAATTTTGCTAACTTTAACCCCATG‑ATTTTTACCCGAAAGCCTTAATAAGTGGGGGGTAAACT‑AAAAAAGATAAAA‑GAAGGCGAAAcagcg                                                                                                                    >  1:652970/1‑235 (MQ=255)

                                                                                                                   taaaTTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                        <  1:263711/202‑1 (MQ=255)

                                                                                                                     aaTAACATATT‑CACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTACCAGACAAATAA‑AAAAACGGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGtc‑gtcg                                                                                                                <  2:595367/239‑1 (MQ=255)

                                                                                                                       ttAAATATT‑CAAGGTGTAAAAAAATATAAAACCGCATTTTAAGTGGTAGTTTGTGGCGGTGAATTTTTCAAGACAAATAC‑AAAAACTGGAGTGGCCAT‑GATGAGCAAAAGTAATAACCAAC‑AG‑‑‑‑‑‑GGA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGAt                                                                                                              <  1:1068744/239‑1 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAC‑AAAAACTGGAGGTGGCAT‑GATGGGCGAAAGGAAAAAACAAA‑AA‑‑‑‑‑‑GCA‑GTGAAAAAATTG‑AAAGAGAGTGTCGCTAACTTTACCGCCATG‑ATTTTTACCCGCATGGCTGATGACGTGGTGGGGAAACT‑AAAAAACCTAAAG‑GATACCGAAAAGTC‑CTCGGTgggg                                                                                                          >  1:631210/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAC‑AAAAAATTGAGTTTTCAT‑GATGAGCGAAAGTAATAAGAAAC‑AG‑‑‑‑‑‑GCA‑GGGAATAAGTTG‑AAAGAGATTGTCGCTAACTTTAACGCCCTG‑ATTTTTACCCCAATGCCTGATGACGTGGTGGGTAAAAT‑AAAAAAGCTAAAG‑GATGACGCAACGCA‑GGCGATgggg                                                                                                          >  1:1388078/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTATAACAAATAA‑AAAAACAGGAGTTGGCAT‑TATGAGGGGAAAAAATAAGAAAC‑AA‑‑‑‑‑‑GAA‑GGGGAAAAGTTT‑AGAGGGAATGTTGCTACATTTATACCCATGAATTATTACCCAAAAGCC‑GGTGTTGTGGGGGATAAAAC‑AAAACAGCTAAAA‑AACTTCGGAAAGTC‑GTCGACgggg                                                                                                          >  2:1019021/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTCAGACAAATAC‑AAAAAAGGGGGGTGCCGT‑GGTGGGCTGGAGTAATAAAAAAC‑AC‑‑‑‑‑‑GAA‑ATAACTAAGTTT‑TCTGACATTTGGGCTAAATTTAAATCAATG‑CGTTCTTCCCCAACCCCTGAGTGCGCCGGGGGTAAAAA‑AAAACAGCTAAAGAGTTTCCTGAAC‑TC‑GTCGATgggg                                                                                                          >  2:229326/1‑239 (MQ=255)

                                                                                                                           atatT‑CACGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGTGGCGGTGATTTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGa                                                                                                                           <  1:1049051/223‑1 (MQ=255)

                                                                                                                           atatT‑CACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTa                                                                                                                                       <  1:829964/212‑1 (MQ=255)

                                                                                                                            tatT‑CACGTGGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTGGTGGCGTTAAATTTTTACATACAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGAGAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTGG‑ACAGAGATTGTCGTTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                         <  1:1403772/239‑1 (MQ=255)

                                                                                                                            tatT‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATAC‑AAAAACGGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGAAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                         <  1:469404/239‑1 (MQ=255)

                                                                                                                            tatT‑CACGGTGGAAAAAAATATAAAACCAAATTTTTAGGGGTAGTTTGGGGCGGTAAATTTTTCCAGACAAAAAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                         <  2:1332086/239‑1 (MQ=255)

                                                                                                                            tatT‑CACGGTGGAAAAAAATATAAAACAACATTTTTAGTGGTAGTTTGGGGGGGTAAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCTAT‑GATGAGCAAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                         <  1:344668/239‑1 (MQ=255)

                                                                                                                            attt‑CACGGTGGAAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAATTGGAGTTGCCTT‑GATGAGCGAAAGTAATAAGCAAC‑GG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                         <  1:1064968/237‑1 (MQ=255)

                                                                                                                             tatccgcGGGGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCAAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGAGAAAGTAAAAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTA‑ACAGATATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                         <  2:251319/233‑1 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTAAACAAAAAC‑AAAAACAGGAGGTTGCAT‑GGTGAGCGGAAGTAATAAACAAA‑AA‑‑‑‑‑‑AAA‑AGGGATAAGATGTACA‑AGAATGTGGCTAAATATACCCCCATA‑ATTTTTTACCGAAAGTATGTTGTAGGGGTGGATTAAAC‑AAAACAACTTAAA‑GAGGCCGCAACCGC‑GTCGGTggggga                                                                                                        >  2:91747/1‑237 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAC‑AAAAAATGGGGGTGCCAT‑GATGGGCGAAAGTAATAAAAAAA‑AA‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAAATTTACCGCCCTG‑ATTTCTACCCGAATGCCTGATGACGGGGGGGGTAAACT‑AAAAACGCTAAAA‑GAAGCCGAAAAGTC‑GGTGATggggga                                                                                                        >  1:1374632/1‑237 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAC‑AAAAAAATGAGTTTGCAT‑GGTGAGCGGAAAAAATAAACAAC‑AA‑‑‑‑‑‑GCA‑AGGAATAAATTT‑ACAAAGAATGTCGCCAACTTTACCGCCCTT‑ATTTTTAACCCAATGGCTGATGACGTGGGGGGTAAAAT‑AAAACAACAAAAA‑GATTCCGCAACATC‑GTCGATggggga                                                                                                        >  1:1232761/1‑237 (MQ=255)

                                                                                                                             atT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGaa                                                                                                        <  1:1151052/239‑1 (MQ=255)

                                                                                                                             aattacgggTGGAAAAAAAAAAAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAAAAC‑AAAACCTGGAGTGGCCAG‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑TTAAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGa                                                                                                         <  1:636384/232‑1 (MQ=255)

                                                                                                                                   cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAA‑AAAAAATTGAGGTGGCCT‑GATTGGGGGAAGTAAAAAACAAA‑AA‑‑‑‑‑‑GCA‑ATGAATAAAATT‑ACAGAGAATGTGGCTAACTTTACCCCCAAG‑ATTTTTATCCCGATGGACGAAAACGGGGGGGGTAAAATAAAAACACTTAAAG‑AATCCGGAAACGTG‑TCCGTGGGGGAAAAta                                                                                                    >  2:487241/1‑238 (MQ=255)

                                                                                                                                    gagaaaaaaaaaTA‑AAAACCAAATTTTTGTGGGTGGTTTGTGAGGGTGAATTTTTCAAAAAAAATAA‑AAAAACGGGAGTTGCCTG‑GATGAGAAAAATAAAAAAACAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAGGTTG‑ACAGAGATTGCCGATAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                                                  <  2:459018/232‑1 (MQ=255)

                                                                                                                                     ggggCAAAAAAAAAAAAACCAAATGTTTAGTGGTAGTTTGGGGCGGTGAATATTTCCAGAAAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAATAAAAAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAATTTG‑ACAGAGATTGTCGCAACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAAGTAt                                                                                                  <  2:576654/236‑1 (MQ=255)

                                                                                                                                     gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                                                  <  1:958061/238‑1 (MQ=255)

                                                                                                                                     gTGGAAAAAAAAAAAAAACCAAATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAA‑AAAACCTGGGGTTGCCAT‑GATGAGCAAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTTTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTAt                                                                                                  <  2:623602/238‑1 (MQ=255)

                                                                                                                                      tGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTCCCAGAAAAATAC‑AAAAACTGGATTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACATCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATc                                                                                                 <  1:134290/238‑1 (MQ=255)

                                                                                                                                           aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAGATTTTCCAAACAAAAAA‑AAAAAATGGAGTTGACCT‑GGTGGGCGGAAGAAAAAAACAAC‑AC‑‑‑‑‑‑ACA‑AAGCAAAAGATG‑GCAGAGATGTTCGATCAATTTACTGCCCTG‑ATTTCTAACCGAAAGCCCGACTACGGGGTGGAGAAACA‑AAAACAGCCAAAG‑GAGGCCCACA‑GTCAGTCGGTGGGGAAAATTATCTACCa                                                                                            >  2:1323481/1‑238 (MQ=255)

                                                                                                                                              aaTATAAAACCACTTTTTTAGTGGTAGTGTGTGGCGGTGAATTTTTCCAGAAAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGTAATAAGCACC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGCCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                       <  1:748633/239‑1 (MQ=255)

                                                                                                                                              aaTATAAAACCACATTTTTAGGGGTAGTTTGTGGCGGTAAATTTTTCCAAAAAAAAAC‑AAAAACGGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTGTCGCAAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                       <  2:1201523/239‑1 (MQ=255)

                                                                                                                                              aaTAAAAACCCACATTTTGAGTGGGAGTTGGGGGGGGTGAATTTTTCCAAAAAAAAAC‑AAAAAGGGGAGTGGCAAT‑GATGAGCGAAAGTAATAAGCAAC‑GG‑‑‑‑‑‑GCG‑GTAAATAAGTGG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                       <  1:229326/239‑1 (MQ=255)

                                                                                                                                              aaTAAAAAACCACTTTTTTAGGGGTAGTTTGTGGGGGTGAATTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCAAT‑GATGAGCGAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GAA‑TGGAATAAGTTG‑ACAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑g                                                                                       <  1:40417/239‑1 (MQ=255)

                                                                                                                                                tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCt                                                                                                                                                                                                        <  2:77967/129‑1 (MQ=255)

                                                                                                                                                          caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAC‑AAAAAATGGAGGTGCCAT‑GATGAGCGAAAGAAATAAACAAC‑AA‑‑‑‑‑‑GCA‑GTGAATAAGATG‑ACAGAGATTGTCGCCAACTTTACCGCCCTG‑ATTTTTACCCCAATGCCTGGTGACGTGGTGGGTAAACT‑AAAACAGCTAAAA‑GATGCCCAAACGAC‑GTCGGTGGGGGAAATTATTTACCCTACAGATGTT‑Gacaaa                                                                           >  1:957057/1‑238 (MQ=255)

                                                                                                                                                          caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAA‑AAAAAATTGAGGTGTCAT‑GGTGAGCGAAAAAAATAAGAAAC‑AG‑‑‑‑‑‑GGA‑GTGAATAAAATG‑ACAAAGATTGTCGCCAACTTTTCCg                                                                                                                                                                                              >  1:77967/1‑129 (MQ=255)

                                                                                                                                                                       ggTTTGGGGTGGAAAATTTTACAAAAAAAATAA‑AAAAACGGAAGTTGCCAT‑GATGAGAGAAAGTAATAAGAAAC‑AG‑‑‑‑‑‑GAA‑GGGAATAAGTTG‑ACAGAGATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                              <  2:652970/238‑1 (MQ=255)

                                                                                                                                                                       aGTTTGTGGCGGTAAATTTTTCAAAAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGGCCGAAAATAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATGGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGa                                                              <  1:1151044/239‑1 (MQ=255)

                                                                                                                                                                             tgGCGGTGAATTTTTCCAGACAAAAAC‑AAAAATTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGCCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGAc                                                         <  1:669602/238‑1 (MQ=255)

                                                                                                                                                                                   taaaTTTTCCCAGAAAAATAA‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAAAAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTGA‑ACAGAGATTGTCGCTACCTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAAt                                                   <  1:147067/236‑1 (MQ=255)

                                                                                                                                                                                                     tAC‑AAAAACTGGAGTTGCCAT‑GATGAGCAAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                <  1:475605/239‑1 (MQ=255)

                                                                                                                                                                                                      taaaaaaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GAA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGaca                                <  1:642776/236‑1 (MQ=255)

                                                                                                                                                                                                         aaaaaCGGGATTTGCCAG‑GATGAGCGAAAGTAATAAGCAAC‑GG‑‑‑‑‑‑GCA‑GTGAATAAGTGG‑ACAGATTTTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCg                             <  1:795382/239‑1 (MQ=255)

                                                                                                                                                                                                           aaaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCggg                           >  2:1250640/1‑239 (MQ=255)

                                                                                                                                                                                                            accgggAGTTGCAAT‑GATGAGGGAAAGAAAAAAGCAAC‑AG‑‑‑‑‑‑GAA‑GGAAAAAGGTTG‑AAAGAGATTGCCGCTACCTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                          <  2:59610/235‑1 (MQ=255)

                                                                                                                                                                                                            aatgtgAGTTGCCGT‑GAGGAGAGAAAGAAAAAAGCAAC‑GG‑‑‑‑‑‑GCA‑GGTAATAAGTGG‑ACAGAGAGTGGCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                          <  2:212011/234‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGGGTTGCAAT‑AAGGAGCAAAAGTAATACGAAAC‑AG‑‑‑‑‑‑GAA‑GTAAAAAAGTTG‑ACAGAGTTTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                          <  2:1349641/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                          <  1:794496/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCCAT‑GATGAGCGAAAGAAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGTTGGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                          <  1:905093/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGGAGTTGCAAT‑GAGGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑AAAGAGATTGTCGCTAATTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                          <  1:582594/239‑1 (MQ=255)

                                                                                                                                                                                                            aaCTGAAGTTGCCAT‑GATGAGCGAAATTAATAAGAAAC‑AG‑‑‑‑‑‑GAA‑GTAAATAAGTTG‑GCAGAGATCGTACCTAATTTAACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                          <  1:793625/239‑1 (MQ=255)

                                                                                                                                                                                                             aCTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGga                         >  2:931448/1‑239 (MQ=255)

                                                                                                                                                                                                                 gAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑CATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCACAAAGCGATTGACCTGACTCGTCCTGCCTGTCAGGCACCCGCGGAGAtt                     >  2:570219/1‑239 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                               >  2:1112121/1‑197 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg     >  1:545939/1‑239 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAg     >  1:561009/1‑239 (MQ=255)

                                                                                                                                                                                                                                  cGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTAAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                               <  1:1112121/197‑1 (MQ=255)

TTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCA‑TTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATT‑CACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAC‑AAAAACTGGAGTTGCCAT‑GATGAGCGAAAGTAATAAGCAAC‑AG‑‑‑‑‑‑GCA‑GTGAATAAGTTG‑ACAGAGATTGTCGCTAACTTTACCGCCATG‑ATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT‑AAAACAGCTAAAG‑GATGCCGAAACGTC‑GTCGATGGGGAAAATTATCTACCATAC‑GATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCG  >  NC_000913/3206249‑3206712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A