Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I0 R1 91 172.1 3477528 97.2% 3380157 226.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,782,913 A→C 18.9% V250G (GTG→GGG)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9130AC18.9% 165.0 / 8.4 101V250G (GTG→GGG) gpsAglycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base A (42/37);  new base C (7/14);  total (49/52)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA  >  NC_000913/3782686‑3783150
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCCACTG‑G‑TTGTCGGTACCAGTAAGCACCAAATCGccc                                                                                                                                                                                                                                        >  1:32318/1‑238 (MQ=255)
tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGGTACGCCACCG‑G‑TTGTTGGTACCAGGAAGCACCAGATCGcc                                                                                                                                                                                                                                         >  1:1129551/1‑238 (MQ=255)
  aaTGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTGATGCGCCGA‑G‑TTGTCGGACAAGGTAAGTCCCAGATCGCCaa                                                                                                                                                                                                                                       >  1:325317/1‑238 (MQ=255)
    tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCAACAG‑G‑TTGTCGGTGCAGGGAAGCACCAGACCGCCaacc                                                                                                                                                                                                                                     >  1:799593/1‑236 (MQ=255)
         ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGGTACGCCACTG‑G‑CTGTCTGACCAGCAAAGAACCAGATAGCCAAG‑cccccc                                                                                                                                                                                                                               >  1:792386/1‑236 (MQ=255)
          cTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTCCCCCCCCCTGACCAATCTTCCCCTGCGCGCTTTGTACATCCAGGCCCGGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGAAAGCACCAGACCGCCAAG‑CCCCGCCa                                                                                                                                                                                                                             <  2:1022941/239‑1 (MQ=255)
           tCGCGCGCGTTTTTTCCGCAATATAATCCTTGATAAATTTCCTCGTTTATGG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGACCGCCAAG‑CCCCGCCAt                                                                                                                                                                                                                            <  2:1447933/239‑1 (MQ=255)
           tCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAt                                                                                                                                                                                                                            <  2:840038/239‑1 (MQ=255)
           tCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGAGGCGCCAGTTCGCGGACTTCTTTCGTATGGCGGTAACCTCCCACCCCCTGACCAATCTCCTCCTGCGCGCTTTGTACACCCATGCCCTGACAGAGCATCATGCAAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGAACAGGTAAGCCCCAGATCGCCAAG‑CCCCGCCAt                                                                                                                                                                                                                            <  2:444633/239‑1 (MQ=255)
           ccgcgcgcgTTTTTCCCCCAATATAAGACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGCCCAACCTTCTCCTGCGCGCTTTGTACATCCATGCCCGGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGATCGCCAAG‑CCCCGCCAt                                                                                                                                                                                                                            <  2:31216/238‑1 (MQ=255)
              cgcgcgTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAACCGGACG‑G‑TTGTCGGGACAAGGGAGCACCAAAAACCCAAG‑CCCCCCCAT‑Gc                                                                                                                                                                                                                         >  1:956769/1‑238 (MQ=255)
                  gcgtttttCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGAGGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCCCCAAG‑CCCCGCCAT‑GCCCAt                                                                                                                                                                                                                     <  1:1534657/235‑1 (MQ=255)
                     tttttCCGAAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTGCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAg                                                                                                                                                                                                                 <  1:1103289/239‑1 (MQ=255)
                          ccGCAATATAATACTTGATAAATTTCCTCGGTTTTTG‑GCATTTCAACGCCGAAGCGCTGCGCAAGTTCGCGGCCTTCTTTCGTATTGCGCTCGCTATCCACCCCATGCCCAATCTTCTCCAGCGCGCTTTGTCCTTCCATGCCCCGACCGCGCCTCATGCCAAAACGCGGGTTACGCGACTG‑G‑TTGTCGGTACGGGTAAGCCCCATAACGCCAAC‑CCCCGCCAT‑GCCCATAAAGGTGGc                                                                                                                                                                                                            <  2:512857/239‑1 (MQ=255)
                               ttataATACTTGAT‑AATTTTCTCGGTTTGTGTGCATTTCAACGCAGAGGCGATGCGCTAGGTCGCGGAATTCTTTCGTATTGCGGTATCCCTCCCCCCCCAGACCAATCTCCTCCTGCGCGTTTTGTACACCCGTGCCCTGACCGAGAAACAGGCAAAACCGGCGGTTTCGCGCATG‑G‑TTGCGGGTAGTGGTAAGCTCCAGAGCGCCAGG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGt                                                                                                                                                                                                       <  2:1297952/238‑1 (MQ=255)
                               ttaaaataCTGTATAAATTCCCCCGGTTTTTG‑CCATTTCAACCCCGAACCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGCCCAACCTCCCCCTGCGCGCTTTGTCCATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTCCGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGt                                                                                                                                                                                                       <  2:1373151/235‑1 (MQ=255)
                                 ataataCTGAATAAATTTCCTCGGTTGTTG‑GCATTTCAAGGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTGCTTATTGCGGTGGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACACCCAGGCCCTACCCGAGCATCAGGCAAAAACGGCGGTTACGGGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCg                                                                                                                                                                                                     <  2:1467852/239‑1 (MQ=255)
                                  taataCTTGATAAATTCCCTCGGTTATTG‑GCATTCCAACGCCGAAGCAATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGc                                                                                                                                                                                                            <  2:522394/231‑1 (MQ=255)
                                     taCTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGACTG‑G‑GTGTCGGGAAAGGGAAGCACCAGAAAGGCCAC‑CCCCGCCCA‑GCCCCCAAAAGGGGCAGGGtgcgca                                                                                                                                                                                                  >  2:173986/1‑233 (MQ=255)
                                        ttGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTCCAGGGCAACACCAGATCGCCCAA‑CCCCCCCCT‑GCCCCCAAACGAGGCAGGGGCGGGAccc                                                                                                                                                                                               >  2:287821/1‑238 (MQ=255)
                                        ttGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACCG‑GTTTGTGGGT‑CAGGGAAGCACCACAACCCCCAG‑CCCCGCCCC‑GCCCCTAAAAGTGGCAGGGGCCGCCCCCa                                                                                                                                                                                              >  2:315228/1‑239 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTCCGCGACCG‑G‑GTTTCGGTACAGGTAAAACCCAAATCGCCAAGACCCCCCCAT‑CCCCCTAAA‑GTTGCAGGGGCCGCAacccc                                                                                                                                                                                             >  2:1498619/1‑236 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCCG‑G‑TTGTCGGTACACGTAAGCACCAAAACGCCCAA‑CCCCGCCCT‑GCCCCTAAAAGCGGCCAGGGCGGCACCCac                                                                                                                                                                                             >  2:748956/1‑237 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTCCAGGTAAGCACCAGAACGCCAAG‑CCCCCCCCT‑CCCCACACAGGTGGCAGGGTCGGCACCCAgc                                                                                                                                                                                            >  1:1347860/1‑239 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAA‑CCCCGCCAT‑GCCCCTAAAGGGGGCAGGGGCGGCACCCAgc                                                                                                                                                                                            >  1:686547/1‑239 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGGACAGGTAAGCCCCAGATCGCCAAG‑CCCCGCCCT‑GCCCATAAAGGGGGCAGGGTCGGCACCCAg                                                                                                                                                                                             >  1:1418459/1‑238 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGGACAGGGAAGCAACAGAACGCCCAG‑CCCCGCCAT‑GCCCCTAAAGGGGGCAGGGTCGGCACCCaac                                                                                                                                                                                            >  1:1152641/1‑237 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑GTGTCGGTCCAGGGAAGCAACAAATCTCCAAA‑CCCCGCCCT‑GCCCCAAAAGGTGGCAGGGGCCGCAcccccc                                                                                                                                                                                            >  1:1261648/1‑236 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACCG‑G‑TTGTCGGTACAGGGAAGCACCAGATCGCCAAA‑CCCCCCCCT‑GCCCACACAGGTGGCAGGGGGGGCACCCac                                                                                                                                                                                             >  2:1515312/1‑237 (MQ=255)
                                          gATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCAACCCCTGACCGAGCATCATGCCAAAACGGCGGCTACCAGACAT‑G‑TTGGCGGGAAACGGAAAACCCAGAACCCAAAG‑CCCCCCCCT‑ACCCCAAACAGTCGCATGGGCGGCAcccca                                                                                                                                                                                             >  2:1632762/1‑236 (MQ=255)
                                            tAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGACTG‑G‑TTGTTGGGACAGGTAAGCACCAGATCGCCCAA‑CCCCGCCCT‑GCCCCTAAAGGGGGGAGGGGCggggccagtc                                                                                                                                                                                            >  1:522394/1‑229 (MQ=255)
                                                    cTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGAACGCCCAG‑CCCCGCCAC‑GCCCCTAAAGGTGGGAGGGGCGGCGCCCCGCGCCGccccca                                                                                                                                                                                  >  1:420288/1‑237 (MQ=255)
                                                       ggTTGTTG‑GCCTTTCAACGCAGAAGCGACGCTCCTGTGCGCGCGCTTCTTTCGTATGGCTGTAGCCTTCCACCCCCCGACCACTTTACTCCTGCGGGTTTTGTACGTCCATGCCCTGCCCGCGCGTCATGCAAAAACGGCGGTTCCGCGACGG‑G‑TTGCCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGa                                                                                                                                                                                <  2:1018646/238‑1 (MQ=255)
                                                         ttAGTG‑GCTTTTCAGCGCCGAAGCGCTGCGCCGGTTCGCGGATTTCTTTCGTATTGCGGTAGCCCTCCCCCACCACACCACTCTCCCCCTGCGCGCTTTGTCCAGCCATGCCCTGACCGACCATCATGCCAAAACGGCGGGTAGGCGCCTG‑G‑TTGTCGGTACAGGTAAGCACCAGACCGCCACG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcg                                                                                                                                                                              <  2:409913/238‑1 (MQ=255)
                                                          tATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTCCGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgcg                                                                                                                                                                            <  1:281463/239‑1 (MQ=255)
                                                          tATTG‑GCATTCCAACGCCGAAGCGATGCGCCAGTGCGCGGATTTCTTTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGAGCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgcg                                                                                                                                                                            <  1:472297/239‑1 (MQ=255)
                                                                     tCACCGCGGAAGCGCGGGGCCAGTTGCTGGCCTGCTTTCGTATTGCGGCAGCCTTCCACCACCGGCCCAATCTCCCCCTGCGCGCTTTGTCAATCCAGGCCCTGACCGAGCAGCATGCCAAAGCGGGGGTTACGCGACTG‑G‑TTTCCGGTACAGGTAGGCCCCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                  <  2:370391/239‑1 (MQ=255)
                                                                     tCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                  <  2:584588/239‑1 (MQ=255)
                                                                     tCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                  <  1:550926/239‑1 (MQ=255)
                                                                          gCCGAAGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTCCCACCCCCGGACCAATCTTCTCCTGCGCGCTTTGTACACCCATGCCCTGCCCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCagccag‑cc                                                                                                                                                            <  1:1507788/239‑1 (MQ=255)
                                                                              aaGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGATCCGCCGCTC‑G‑TTGTCTGGCCAGGGAAGAACCCAATCGCCAAA‑CCCCGCCCA‑CCCCCTCAAAGTGGCAGGGCCCGACCCCAGCGCCGCCCCCCGCCGCGCCACTTcagcccacccccg                                                                                                                                                         >  2:1350017/1‑231 (MQ=255)
                                                                               aGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATGGCGGTAGCCTTCCCCCACCTGACCAATCTCCTCCTGCGCGCTTTGTACATCCAGGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACgg                                                                                                                                                       <  1:941525/239‑1 (MQ=255)
                                                                                  gaggggcgcgTTCGCGACCTTCTTTCGTATGGCGGTGGCCTTCCCCCCCCTGCCAAATCTTCTCCGGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGGTTACGCGACTG‑G‑TTGCCGGTAGAGGTAAGCACCAGACCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGt                                                                                                                                                     <  2:542595/229‑1 (MQ=255)
                                                                                        ccAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCCACTG‑G‑TTGTCGGTACAAGGAAGCACCAAATCGCCCAA‑CCCCCCCCA‑GCCCCTAAAAGGGGGAGGGGCGGGCCCCCGCGCCCGCCCAAGACGCCACAATTTAGACAGACCCACGGGGGacag                                                                                                                                                >  2:388431/1‑235 (MQ=255)
                                                                                         gtgTTCGCGGACTTTTTTCGTAGTGCGGCAGCCTCCCACGTCCGGACCAGCCTCCCCCGGCTCACTTTGTACATCACGGCCGTCACCGGTCAAGACGCCAAACGGGGGTTTCCGATTCTG‑G‑TTGTCGTCACGTGAAAGCCCAAGCCCGCAACG‑CCCCGCCAT‑GCCCATAAAGGGGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAgc                                                                                                                                              <  2:1129551/236‑1 (MQ=255)
                                                                                         cAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAgcg                                                                                                                                             <  1:1049431/239‑1 (MQ=255)
                                                                                                      tcttTCGTATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                <  2:640016/239‑1 (MQ=255)
                                                                                                      tcttTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGCCCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                <  1:1368373/239‑1 (MQ=255)
                                                                                                      tcttACGAATTGGGGTACCCTCCCACCCCCTGACAAATTTTCACCGGCGCGCTTTGTACCTCCAGGCCCTGACCGAGCAACATCCCAAAACGGCGGTTACGCGATTG‑G‑TTGTCGGCACAGGAAAGCCCCAGATCCCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                <  2:206035/239‑1 (MQ=255)
                                                                                                            tttttGCGGTAGCCTTCCCCCACCTGCCCATCCTCCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCCCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑cc                                                                                                                         <  2:1418459/236‑1 (MQ=255)
                                                                                                            gTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑cc                                                                                                                         <  1:748956/239‑1 (MQ=255)
                                                                                                                              cccaccTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGCCCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGa                                                                                                       <  1:937185/238‑1 (MQ=255)
                                                                                                                              accaccTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGa                                                                                                       <  1:369594/239‑1 (MQ=255)
                                                                                                                               ccaccTGCCCAATCTTCCCCTGCGCGCTTTGTACACCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGCCGGTACAGGTAAGCACCAGACCGCAAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGAc                                                                                                      <  1:1515312/239‑1 (MQ=255)
                                                                                                                                      aCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGGCTG‑G‑TTGTCGGGACAGGGAAAGACCAAAACGCCCAG‑CCCCGCCCC‑GCCCATAAAGGGGGGCGGGGCGGGACCCAGCGCCCCACCAACACGCGACCTTTTAGCCCC‑CCCACGGGGGAGCAGCGCGGGGACCGCCTTCGCC‑CCCAAACCACCACCGCCGGACACcccc                                                                                                >  2:811197/1‑238 (MQ=255)
                                                                                                                                          aTCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACGG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCAcc                                                                                           <  1:741261/239‑1 (MQ=255)
                                                                                                                                             ttctCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGTCTG‑T‑TTGTCGGTACAGGTAAGCACCAGATCGCCAGG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAAt                                                                                        <  1:183412/239‑1 (MQ=255)
                                                                                                                                                tcCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTG‑G‑TTGTCGGTACAGGTAAGCACCACATCGCCAAA‑CCCCCCCAT‑GCCCCTAAAGATGGCAGGGTCGGGACCCAGCGCCGCCCCACAACGCGACATTTCAGCCAG‑CCCCCGGGCGATCAGCGCCGTACGCGCCTTCGCC‑CCAACACCCATCCCCCCGGACATACCCCCCCCCATGGc                                                                                     >  2:1189962/1‑239 (MQ=255)
                                                                                                                                                tcCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGACTG‑G‑TTGTCGGGAACGGGAAGCACCAGAACGCCCAG‑CCCCCCCCTCCCCCCTAAAGG‑GGCACGGGCGGCAACCAGCCCCCCCCCCCAACGCGCCCTTTCCACCCG‑CCCACGGGGGAGCACCGCCGGAAGGGCCTTCGGC‑CCACAACCCACACCCGCGGACACCCCCGcccccaagg                                                                                      >  2:90172/1‑233 (MQ=255)
                                                                                                                                                  cTGCGCGTTTTGTGCACCCACGCCCTGCCCGAGCAACATCCCAAAGCGGCGGTTACCCGCGTGTG‑TTG‑CGGTACAGGTAAGCACCAGACCCCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCaa                                                                                   <  2:1261648/239‑1 (MQ=255)
                                                                                                                                                     cgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCCCGACTG‑G‑TTGTCGGTACAGGGAAGCCCCACAACGCCAAG‑CCCCCCCAT‑GCCCCTAAAGGGGGCAGGGGCGGCCCCCCCCGCCGCCCCCAAACGCGACACTTCAGCCCG‑CCCCCCGGTGATCAGCGCCCTACGCGCCTTCCCC‑CCCAAACCCACACCGTCCGACAACCCCGcccccaccgcaacca                                                                                >  2:1296824/1‑228 (MQ=255)
                                                                                                                                                          tttGTACATCCATGCCCTGACCGGGCATCATGCCAAAACGGCGGTTCCGCGACTG‑G‑TTGTCGGTACAGGTAACCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGa                                                                                                                 <  1:1367048/201‑1 (MQ=255)
                                                                                                                                                            tGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGc                                                                                                                                                                                                            <  1:1098249/110‑1 (MQ=255)
                                                                                                                                                                 ctCCATGCCCTGACCGAGCATCATGCCAAAACGGCGTTTCCGCTACTG‑G‑TTGTCGGTACAGGTAACCACCAGACCGCCAGG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg                                                                    <  1:1210267/238‑1 (MQ=255)
                                                                                                                                                                  tCCAGGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcgc                                                                   <  1:7026/239‑1 (MQ=255)
                                                                                                                                                                    cATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTAAAGGTAAGCACCAAAACGCCAAG‑CCCCGCCCC‑GCCCCTAAAAGTGGCAGGGTCGGCACCCCGCGCCCCCCCCAAACGCCACCTTTCAACCCG‑CCCCCCGGGGAGCCGCGCCGCACGCCCCTTCGCA‑CCAAAAACCACACCCCCGGCCCTCCCCCCCCCCACGGCAAACAAGCTTTTCaacacc                                                                  >  2:1290639/1‑233 (MQ=255)
                                                                                                                                                                      tGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGACTG‑G‑GTTTCGGCACAGAGAAAAACCAAAACGCCAAA‑CCCCCCCCT‑TCCCATAAAGGGGGCCAGGGCGGCACCCCCCGCCGCCCCCAAACGCGCCATTTTAGCCCC‑CCCCCGGGGGGTTCACGCCGCAAGCGCATTCGCACCCAAAACCGCCA‑CGCCGGACACCCCCCCCCCCACGGCCCAAACCGTTTTCACCCcgcc                                                                >  2:1306131/1‑238 (MQ=255)
                                                                                                                                                                           ggACCGGGCATCAGGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCCCCAGACCCCCCAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAg                                                          <  2:686547/238‑1 (MQ=255)
                                                                                                                                                                             aCCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑GTGTCGGTACAGGGAAGCCACAAAACGCCAAA‑CCCCCCCAC‑GCCCATAAAGGGGGCAGGGGCGGCACCCCGCGCCCCCCCCAGACCCCACATTTCCGCCAG‑CCCCCCGGGGATCAGCGCCCCACCCCCACTCCCA‑CCAAAACCGCAACCCCCGGCACTCCCCCCACCCATGGCCAAAAAGCTTTTCACCCCCCCCCCaaca                                                         >  2:422204/1‑236 (MQ=255)
                                                                                                                                                                              ccaggCATCAGCCAAAAACGGCGGTTCCGCGACTG‑T‑TTGTCGGTACGGGAAAGCCCCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTg                                                       <  1:1433297/235‑1 (MQ=255)
                                                                                                                                                                                gggCATCAGGCCAAAACGGCGGTTACGTGACTG‑G‑TTGCCGGTACAGGAAAGCACCAGACCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCa                                                     <  1:1306131/237‑1 (MQ=255)
                                                                                                                                                                                   catcatGCCAAAACGGCGGTTACGCGACTG‑G‑TTTTCGGGACAGGGAAGCACCAAAACGCCCAA‑CCCCCCCAT‑GCCCCCAAAGGGGGGAGGGGCGGCACCCAGCCCCGCACCCAAACGCCACCATTTAGCCAG‑CCCACCGGGGATCAGCGCCGCACCCCCCTTCGCA‑CCAAAACCCAAACCGCCCGACATCCCCCCCCCCAAAGGAATAAAGTTTTTTCCCCCgccccccacaccgccg                                                   >  2:714395/1‑228 (MQ=255)
                                                                                                                                                                                    atcagGCCAAGACGGCGTTTACGCGACTG‑G‑TAGTCGCTAGAGGTAAGCACCACGTCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGACCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACAGCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGc                                                  <  2:1544992/238‑1 (MQ=255)
                                                                                                                                                                                    ataatGCCAAACCGGGGGTTCCGCGCCTG‑T‑TTGCCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGc                                                  <  1:91101/235‑1 (MQ=255)
                                                                                                                                                                                    accatGCCAAAAGGGCGGTGACGCGCCGG‑G‑TTGTCGTTACGGGTAAGCACCAGACCGCCAGG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACg                                                   <  1:1287826/235‑1 (MQ=255)
                                                                                                                                                                                     caagcccAAACCGGCGTGTCCCCGACTG‑G‑TGGCGGGTACAGGAAGCCACCAGACCGCAAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCa                                                <  2:698291/234‑1 (MQ=255)
                                                                                                                                                                                            aaaaCGGCGGTTACGCGAGTG‑G‑TTGTCGGTCAGGGAAAGCCCCAGATCGCAAAG‑CCCCGCCAT‑GCCCAGAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAAt                                         <  1:1350017/239‑1 (MQ=255)
                                                                                                                                                                                                  gcggTTCCGCGACTG‑G‑TTGGCGGTACAGGTAAGCACCAGACCGCCAAC‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGa                                    <  2:1614182/238‑1 (MQ=255)
                                                                                                                                                                                                         cgcgCCTG‑G‑TGGCCGGTACAGTTAAGCCCCAGACCCACAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTa                            <  1:388431/239‑1 (MQ=255)
                                                                                                                                                                                                                     tgtgGGTACAGGTACGCCCCAGATCGCCCAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGaa                   <  1:614074/238‑1 (MQ=255)
                                                                                                                                                                                                                           tACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACtttt             >  1:8953/1‑238 (MQ=255)
                                                                                                                                                                                                                             cAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc          >  1:1221127/1‑239 (MQ=255)
                                                                                                                                                                                                                              aGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgcg                                                                                                                                                                            <  2:833833/78‑1 (MQ=255)
                                                                                                                                                                                                                              aGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGAcgcg                                                                                                                                                                            >  1:833833/1‑78 (MQ=255)
                                                                                                                                                                                                                              aGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc          >  1:1390119/1‑238 (MQ=255)
                                                                                                                                                                                                                                gTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCa       >  1:293689/1‑239 (MQ=255)
                                                                                                                                                                                                                                gTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCACTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACAAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTACACGCGGCAACTTTTGccccc       >  2:254815/1‑236 (MQ=255)
                                                                                                                                                                                                                                 tAAGCACCAGACCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCg         <  1:80808/236‑1 (MQ=255)
                                                                                                                                                                                                                                   aGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGt     >  1:1087891/1‑238 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCACTCACGTTTTTCACCGCGCCGCCAAGCTGCCCGCCCATGACATCCGGATTGCTGTAAACTCGGAACCTTTTGCCGCAGTgc   >  2:552120/1‑238 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGCTTGCTGTAAACGCGGAAACTTTTGCCGCCGTgca  >  2:1245573/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:220604/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:1548081/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:946404/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:370052/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:767670/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:251632/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:1502381/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:1250502/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:1223339/1‑239 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 
TCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTG‑GCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTG‑G‑TTGTCGGTACAGGTAAGCACCAGATCGCCAAG‑CCCCGCCAT‑GCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAG‑CCCACGGGTGATCAGCGCCGTACGCGCATTCGCA‑CCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA  >  NC_000913/3782686‑3783150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A