Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F15 I1 R1
|
8 |
40.3 |
1456528 |
96.3% |
1402636 |
133.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
756,830 |
(G)5→4 |
coding (924/1767 nt) |
sdhA → |
succinate dehydrogenase, flavoprotein subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 756,826 | 0 | G | . | 100.0%
| 142.8
/ NA
| 32 | coding (920/1767 nt) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (16/16); total (16/16) |
TGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGT > NC_000913/756700‑756965
|
tgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaac > 6:136324/1‑149 (MQ=255)
aacaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTc > 2:99897/1‑149 (MQ=255)
acaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactga < 4:47261/143‑1 (MQ=255)
acaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactga > 3:47261/1‑143 (MQ=255)
acaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCg < 2:96899/149‑1 (MQ=255)
caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaa > 1:195980/1‑144 (MQ=255)
caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaa < 2:195980/144‑1 (MQ=255)
caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGa < 5:136324/149‑1 (MQ=255)
aaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGAt < 7:55296/149‑1 (MQ=255)
aacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGGTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATc < 8:63201/149‑1 (MQ=255)
gAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCt > 1:152635/1‑145 (MQ=255)
gAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCt < 2:152635/145‑1 (MQ=255)
ggAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaa < 6:18052/123‑1 (MQ=255)
ggAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaa > 5:18052/1‑123 (MQ=255)
ggCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtc < 1:149339/145‑1 (MQ=255)
ggCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtc > 2:149339/2‑146 (MQ=255)
gCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtctc < 8:17768/131‑1 (MQ=255)
gCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtctc > 7:17768/1‑131 (MQ=255)
cAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTAt > 2:21846/1‑149 (MQ=255)
gTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCt > 8:8070/1‑149 (MQ=255)
gcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGc > 2:39298/1‑127 (MQ=255)
gcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGc < 1:39298/127‑1 (MQ=255)
atcatGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg > 1:195967/1‑149 (MQ=255)
aTCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTccc > 5:74305/1‑120 (MQ=255)
aTCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTccc < 6:74305/120‑1 (MQ=255)
tCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGa < 1:99897/149‑1 (MQ=255)
aGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt > 4:108536/1‑131 (MQ=255)
aGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt < 3:108536/131‑1 (MQ=255)
tgtgATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg < 6:186999/105‑1 (MQ=255)
tgATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTg < 1:21846/149‑1 (MQ=255)
gATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGt > 8:161954/1‑149 (MQ=255)
gATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGt > 1:142504/1‑149 (MQ=255)
|
TGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGT > NC_000913/756700‑756965
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTC > NC_000913/756702‑756966
|
AACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAA > NB501016:228:HFK2FAFXY:2:11209:17113:19024/1‑145 (MQ=60)
ACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGAT < NB501016:228:HFK2FAFXY:3:21504:10030:17747/151‑1 (MQ=60)
ACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAAC > NB501016:228:HFK2FAFXY:1:21308:13078:14818/1‑146 (MQ=60)
CAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATC < NB501016:228:HFK2FAFXY:4:11512:2383:13245/151‑1 (MQ=60)
CGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTG > NB501016:228:HFK2FAFXY:1:21205:7726:8837/1‑147 (MQ=60)
TGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAAC > NB501016:228:HFK2FAFXY:3:11408:22156:1174/1‑125 (MQ=60)
GGGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCT < NB501016:228:HFK2FAFXY:1:21204:12125:6322/148‑1 (MQ=60)
CGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCG > NB501016:228:HFK2FAFXY:4:11408:7530:9859/1‑133 (MQ=60)
CGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCT < NB501016:228:HFK2FAFXY:1:11202:7276:6098/129‑1 (MQ=60)
CATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGG > NB501016:228:HFK2FAFXY:1:21308:11188:14694/1‑151 (MQ=60)
GATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCG > NB501016:228:HFK2FAFXY:3:11604:18770:20254/1‑122 (MQ=60)
AGCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGAT < NB501016:228:HFK2FAFXY:1:11311:7041:6465/151‑1 (MQ=60)
AAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTG < NB501016:228:HFK2FAFXY:2:21111:8868:16371/133‑1 (MQ=60)
CTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCagatcggaagaagcacacgtctgaactccagtcactccgcgaa > NB501016:228:HFK2FAFXY:3:21612:16938:10084/1‑107 (MQ=60)
GTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGT < NB501016:228:HFK2FAFXY:1:11108:20926:5995/151‑1 (MQ=60)
TGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTC > NB501016:228:HFK2FAFXY:1:21201:5689:18429/1‑151 (MQ=60)
GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCC < NB501016:228:HFK2FAFXY:3:11406:18786:5675/132‑1 (MQ=60)
|
AACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTC > NC_000913/756702‑756966
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
