Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F7 I1 R1
|
8 |
39.7 |
1405716 |
96.7% |
1359327 |
136.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
757,572 |
C→T |
H556Y (CAC→TAC) |
sdhA → |
succinate dehydrogenase, flavoprotein subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 757,572 | 0 | C | T | 100.0%
| 65.9
/ NA
| 20 | H556Y (CAC→TAC) | sdhA | succinate dehydrogenase, flavoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/9); total (11/9) |
GAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGC > NC_000913/757440‑757715
|
gAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGc < 6:141835/148‑1 (MQ=255)
ccTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGCGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGAcgc > 6:90713/1‑149 (MQ=255)
tGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCg > 1:2875/1‑148 (MQ=255)
aaCGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCg > 8:70476/1‑149 (MQ=255)
gCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCTTCGGCGCGTCat < 1:101229/149‑2 (MQ=255)
cTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGaaa < 2:2875/149‑1 (MQ=255)
tGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGcc > 1:149297/1‑149 (MQ=255)
ccAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGcc < 7:70476/147‑1 (MQ=255)
ccGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGCATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTc > 5:175274/1‑149 (MQ=255)
tGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGTCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGta < 2:149297/149‑1 (MQ=255)
ttCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGa > 4:25649/1‑148 (MQ=255)
ttCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGa > 6:33450/1‑148 (MQ=255)
cccGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGaaa < 3:25340/148‑1 (MQ=255)
cccGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGa < 5:33450/146‑1 (MQ=255)
atgaAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGttt > 4:90443/1‑149 (MQ=255)
gaAAACTGGCTGTGCTACTCCCTGCATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGtttt < 6:175274/148‑1 (MQ=255)
aaaCTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCaa > 4:137532/1‑149 (MQ=255)
aaCTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCg > 5:82848/1‑118 (MQ=255)
aaCTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCg < 6:82848/118‑1 (MQ=255)
tgtgCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGc > 8:148425/1‑149 (MQ=255)
|
GAGTGCCTGGAACTGGATAACCTGATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAATGAGACTCGAGTTTTCAATTTATCGC > NC_000913/757440‑757715
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAA > NC_000913/757464‑757689
|
ATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGA > NB501016:228:HFK2FAFXY:1:11101:21764:19748/1‑150 (MQ=60)
GGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCttcggcgcgtcatg < NB501016:228:HFK2FAFXY:1:11312:21611:16364/151‑15 (MQ=60)
CTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCC > NB501016:228:HFK2FAFXY:1:21206:8294:4120/1‑151 (MQ=60)
GCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGAGCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCC < NB501016:228:HFK2FAFXY:4:11608:6403:11374/149‑1 (MQ=60)
GCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGCATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCG > NB501016:228:HFK2FAFXY:3:21610:12820:14160/1‑151 (MQ=60)
TCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAA < NB501016:228:HFK2FAFXY:2:11112:8279:17797/150‑1 (MQ=60)
TCCCGGATCGTGATGATGAAAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAA < NB501016:228:HFK2FAFXY:3:11502:17770:7113/148‑1 (MQ=60)
AAACTGGCTGTGCTACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGG > NB501016:228:HFK2FAFXY:3:11609:14040:7570/1‑120 (MQ=60)
|
ATGGAAACGGCGTATGCAACGGCTGTTTCTGCCAACTTCCGTACCGAAAGCCGTGGCGCGCATAGCCGCTTCGACTTCCCGGATCGTGATGATGAAAACTGGCTGTGCCACTCCCTGTATCTGCCAGAGTCGGAATCCATGACGCGCCGAAGCGTCAACATGGAACCGAAACTGCGCCCGGCATTCCCGCCGAAGATTCGTACTTACTAATGCGGAGACAGGAAAA > NC_000913/757464‑757689
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
