Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 578,196 A→C 39.4% A30A (GCA→GCC)  rzpD → DLP12 prophage; putative murein endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913578,1960AC39.4% 80.0 / 66.0 76A30A (GCA→GCC) rzpDDLP12 prophage; putative murein endopeptidase
Reads supporting (aligned to +/- strand):  ref base A (22/24);  new base C (17/13);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAA  >  NC_000913/578050‑578342
                                                                                                                                                   |                                                                                                                                                  
gAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAAt                                                                                                                                                  <  8:274063/149‑1 (MQ=255)
  aaGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCAGTCAATCATTACCGTGATAACG‑CAATCg                                                                                                                                                >  7:220323/1‑149 (MQ=255)
     gCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATAATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGcc                                                                                                                                              >  4:317541/1‑148 (MQ=255)
         gatgaCTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGAATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACa                                                                                                                                          >  6:187840/1‑148 (MQ=255)
          atgaCTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACaaa                                                                                                                                        <  6:123472/149‑1 (MQ=255)
                 gtcgtGAGATTGAGCGTGAAGTCTCTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGATCAATAATTAACTTGATAAAT‑CAATAGCCTACAAAGTCAAgc                                                                                                                                 >  7:353498/1‑149 (MQ=255)
                          ttGAGCGTGAAGTCTGTTTGTGCCGCCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTCATTATCTGCATCATCGTCAGCCTGTCATGGGCGGGCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCACCGCGACaaa                                                                                                                         >  5:4801/1‑148 (MQ=37)
                                 tGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGc                                                                                                                    >  3:255292/1‑146 (MQ=255)
                                   aaGTCTGTTTGTTGGGGCAACAGGGAGCAGAGTCACCGCGATTATATACGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCaga                                                                                                                <  6:105064/148‑1 (MQ=255)
                                     gtattgttGTGGGGGCAACAGTGAGCAGAGTAACCGAGAGTATATCCGCTCTGATTATCTGCATCATCTTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGaa                                                                                                             <  2:199497/143‑1 (MQ=25)
                                                ggggCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGc                                                                                                  <  4:255292/149‑1 (MQ=255)
                                                   gCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGaa                                                                                               <  5:148594/149‑1 (MQ=255)
                                                    gatgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGCTTATCTGCATCATCGTCTGCCTGTCATGGTCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAAc                                                                                              >  2:272482/7‑149 (MQ=11)
                                                     atgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGc                                                                                                                    <  3:203957/121‑1 (MQ=11)
                                                     atgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                                                                                                                  >  4:315251/6‑128 (MQ=11)
                                                     atgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                                                                                                                  <  3:315251/123‑1 (MQ=11)
                                                      tgtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCCTCGTCTGCCTGTCATGGGCGGTTTATCATTCCCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAAcgc                                                                                            >  3:300436/5‑149 (MQ=11)
                                                       gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCaga                                                                                                                >  1:183910/4‑128 (MQ=11)
                                                       gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCaga                                                                                                                <  2:183910/125‑1 (MQ=11)
                                                       gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAAc                                                                                                            <  1:217212/129‑1 (MQ=11)
                                                       gtatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAAc                                                                                                            >  2:217212/4‑132 (MQ=11)
                                                       cAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAAcgcg                                                                                           >  7:344002/1‑149 (MQ=255)
                                                        tatGAGCAGAGTCACCGCGATTATCTCCGCTCTGTTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATACCG‑GCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAAcgcg                                                                                           <  6:191777/146‑1 (MQ=11)
                                                        tatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                                                                                                                  >  6:105395/3‑125 (MQ=11)
                                                        tatGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCa                                                                                                                  <  5:105395/123‑1 (MQ=11)
                                                         gTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCgg                                                                                          >  6:174608/1‑148 (MQ=255)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGTTTATCTGCATCATCGTCTGCCTGTCATTGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGc                                                                                         <  6:109236/148‑1 (MQ=11)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                                                                                                               >  3:311728/1‑126 (MQ=11)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                                                                                                               <  4:311728/126‑1 (MQ=11)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCtg                                                                                                    >  3:230302/1‑136 (MQ=11)
                                                          tGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCtg                                                                                                    <  4:230302/137‑2 (MQ=11)
                                                           gAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGa                                                                                                         <  7:210068/131‑1 (MQ=11)
                                                           gAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGa                                                                                                         >  8:210068/1‑131 (MQ=11)
                                                           gAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCtg                                                                                                    <  2:218203/136‑2 (MQ=11)
                                                            aGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                                                                                                               <  6:6182/124‑1 (MQ=11)
                                                            aGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCagag                                                                                                               >  5:6182/1‑124 (MQ=11)
                                                            aGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCAGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAAt                                                                                      <  8:220323/149‑1 (MQ=255)
                                                             gCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAgcgc                                                                                                                               >  8:65908/1‑107 (MQ=11)
                                                               agagTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCTGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGc                                                                                                  <  6:91822/134‑1 (MQ=255)
                                                               agagTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGc                                                                                                  >  5:91822/1‑134 (MQ=255)
                                                                      ccGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATg                                                                            >  2:96416/1‑149 (MQ=11)
                                                                       cgcgATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATg                                                                            >  8:169899/1‑148 (MQ=255)
                                                                                ctCCGATCTGGTTATCTGCATCATCGTCTGCCTGTCATGGGCTGTTAATGATTACCGAGATAACG‑ACATTACCTACAACGCCCAGCGCGCCAAAAATGCAAGAGAACTGAAGCTGGAGCACGCGGCAATTACTGACATGAAGATGCGt                                                                   >  6:236726/2‑148 (MQ=255)
                                                                                    gCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCTAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCg                                                              <  8:103696/149‑1 (MQ=255)
                                                                                        tGATTATCTGCATCATCGTCACCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑GATTCGCCTACAATGTCCAGCGCGACAAAAATGCCAGAGATCTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGAt                                                          <  6:4801/149‑1 (MQ=17)
                                                                                          gtgaTCTTCATCATCGTCTGCCTGTCATGGGCGGGTAATCATTACCGTGATAACG‑CCATTACCTAAAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGt                                                        <  4:188766/146‑1 (MQ=11)
                                                                                          aTTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGt                                                        >  1:106550/1‑149 (MQ=255)
                                                                                                  catcatCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTtgct                                                    <  5:174608/145‑1 (MQ=255)
                                                                                                  catcatCGTCAGCATGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTgcgc                                                >  8:153312/1‑149 (MQ=255)
                                                                                                   atcatcGTCAGCCTGTCATGGGCTGTAAATCATTACCGTGATAAAG‑CCATTACATACAAAGCCCATCGCGACAAAAATGCAAGAGAACTGAACCTGGCTAACACGGCAATAACTGACATGCAGATGCGTCAGAGTGATGTTGCTGCGCt                                               >  8:249689/1‑149 (MQ=255)
                                                                                                    tcatcGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTc                                              >  7:305095/1‑149 (MQ=255)
                                                                                                     catcGTCTGCCTGTCATGGGCTGTTAATCATTACCGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCACAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCTGCGTGATGTTGCTGCGCTc                                              >  6:382667/1‑148 (MQ=11)
                                                                                                     catcGTCTGCCTGTCATGGGCTGTTAATCATTAACGTGATAACG‑CCATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGATAACTGAAGCTGTCTAACTAGGCAATTACTGACATTCAGATGAGTCAGAGTGATGTTTCTGAGCTc                                              >  6:67882/1‑148 (MQ=255)
                                                                                                      atcGGCAGCCTGTCATGGGCGGTCTATCTTTACCGTGATCTGT‑CATTCGCGTACTTAGTCCAGCGCGTGATATATGCCAGTGAACTGATGCTAGCGATCGGGGCTATTACTGACATGCATATGCGTCAGCGTGATGTTGCTGCGCTCgt                                            <  6:71059/149‑2 (MQ=12)
                                                                                                       tcGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGAt                                           <  6:133371/149‑1 (MQ=255)
                                                                                                             gCCTGTCATGGGCGGTCAATCATTAGCGTGATAACG‑CAATCGCCTACAAAGTCCTGCGCGACAAAACTGCCAGAGCACTGAAGCTAGCGATCGCGGCAATTACTGACCTGCAGATGCGGCAGCGTGATGTTGCTGCGCTCGATGCaaaa                                     <  2:278439/149‑1 (MQ=17)
                                                                                                                  tCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAg                                                                         >  6:347098/1‑108 (MQ=255)
                                                                                                                  tCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAg                                                                         <  5:347098/108‑1 (MQ=255)
                                                                                                                  tCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATac                                  <  5:187840/147‑1 (MQ=255)
                                                                                                                       ggatgttaaTCATTACCGTGATAACG‑CAATTACCTACAAAGCCCAGCGCGACAAAAATGCCAGAGAAATGAAGCTTGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAg                            <  1:97848/141‑1 (MQ=11)
                                                                                                                       ggCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAg                            <  2:106550/148‑1 (MQ=255)
                                                                                                                        gCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAg                            >  2:314392/1‑147 (MQ=255)
                                                                                                                          ggTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGAGACAAAAATGCCAGAGACCTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGt                        <  5:341392/149‑1 (MQ=255)
                                                                                                                            tCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTa                      <  7:352744/149‑1 (MQ=255)
                                                                                                                            tCAATCATTACCGTGATAACG‑CAATCGACTACAAAGTCCAGCGCGAAAAAAATGCCAGAGAACTGAAGATAGCGAACGCGGCAATTACTGACATGAAGATGCGTCAGCTTGATGTTGCTGCTCTCGATGCAAAATACACGaa                             >  8:217084/1‑142 (MQ=14)
                                                                                                                                  aTTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAATCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGAt                <  3:317541/149‑1 (MQ=255)
                                                                                                                                  aTTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGa                 <  7:169899/148‑1 (MQ=255)
                                                                                                                                   ttACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGAAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATg               >  4:359584/1‑149 (MQ=255)
                                                                                                                                   ttACAGTGATAACGACAAT‑ACATACAAATCCCAGCGAGACAAAAAAGCCAGAGAACTGAAGCTGGCGAACGAGGCAATTACTGACAAGCAGATGCGTCAGAGAGAAGTTGCTGCGATAGATGAAAAATACACGAAGGAGTTAGCTGAt                >  4:314430/1‑148 (MQ=2)
                                                                                                                                    tACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGc              >  6:149577/1‑149 (MQ=255)
                                                                                                                                        gTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGaaa          <  8:305095/149‑1 (MQ=255)
                                                                                                                                         tGATAACG‑CCATTACATACAAAGCCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTCGCGAACGCGGCAATTACTGACATGAAGATGCGTCAGCGTGATCTTCCTGCCCTCGAAGCAAAATCCACGAAGCAGTAAGCTGATGATAAAg         >  4:283889/1‑149 (MQ=11)
                                                                                                                                           aTAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCt       >  2:165247/1‑149 (MQ=255)
                                                                                                                                               cG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTa                      >  2:303938/1‑130 (MQ=255)
                                                                                                                                               cG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTa                      <  1:303938/130‑1 (MQ=255)
                                                                                                                                                g‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGaaaa  >  7:225440/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GAAAGGCCTGATGACTCGTCGTGAGATTGAGCGTGAAGTCTGTTTGTGGGGGCAACAGTGAGCAGAGTAACCGCGATTATATCCGCTCTGATTATCTGCATCATCGTCAGCCTGTCATGGGCGGTCAATCATTACCGTGATAACG‑CAATCGCCTACAAAGTCCAGCGCGACAAAAATGCCAGAGAACTGAAGCTAGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGCGTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCGAAAGCTGAAAA  >  NC_000913/578050‑578342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A