Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 581,042 T→G 100% L70R (CTG→CGG)  nohD → DLP12 prophage; DNA packaging protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913581,0420TG98.6% 6356.6 / 33.3 2453L70R (CTG→CGG) nohDDLP12 prophage; DNA packaging protein
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base G (1034/1373);  total (1056/1397)
Fisher's exact test for biased strand distribution p-value = 3.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 2470 total aligned reads displayed.

CTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCT  >  NC_000913/580896‑581186
                                                                                                                                                  |                                                                                                                                                
cTGGCAGGAACAGGGAATGCCAGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACtgcggc                                                                                                                                                <  3:7968/149‑1 (MQ=21)
   gCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCa                                                                                                                                               <  7:151480/147‑1 (MQ=21)
     aGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGc                                                                                                                                            >  5:370748/1‑148 (MQ=21)
     aGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGc                                                                                                                                            >  6:176463/1‑148 (MQ=21)
       gAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGC‑TTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTCCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGc                                                                                                                                        >  2:132718/1‑149 (MQ=255)
        aaCAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGc                                                                                                                                        >  6:341942/1‑149 (MQ=21)
         aCAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGc                                                                                                                                        >  2:244664/1‑148 (MQ=21)
          cAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCga                                                                                                                                      <  3:58536/149‑1 (MQ=21)
           aGGGAATGCCCGTTCTGCGAGTCGGTGGCAAGGGTAATGAGGGGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGGTGAAGAACTGCGGCAGGCCAGCgag                                                                                                                                     <  7:368422/149‑1 (MQ=255)
           aGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAGTTGAGAACGAACATCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCgag                                                                                                                                     >  2:263619/1‑149 (MQ=255)
           aGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCgag                                                                                                                                     >  8:66332/1‑149 (MQ=21)
           aGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCgag                                                                                                                                     >  1:96946/1‑149 (MQ=21)
            gggAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCgagg                                                                                                                                    >  5:345745/1‑148 (MQ=21)
            gggAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCgagg                                                                                                                                    <  5:216800/149‑2 (MQ=21)
             ggAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCgaggc                                                                                                                                   <  1:334699/149‑3 (MQ=21)
             ggAATGCCCGTTCTGCGAGGCGGTGGCAAGGCTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTCAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCgagg                                                                                                                                    >  8:254409/1‑147 (MQ=255)
                 tGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCgagg                                                                                                                                    >  1:372264/1‑143 (MQ=21)
                 tGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAt                                                                                                                               <  7:184556/149‑1 (MQ=21)
                 tGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAt                                                                                                                               <  2:87892/149‑1 (MQ=21)
                 tGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAt                                                                                                                               <  8:223951/149‑1 (MQ=21)
                 tGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTGGAAGAACTGCGGCAGGCCAGCGAGGCAGAt                                                                                                                               <  1:285931/149‑1 (MQ=255)
                 tGCCCGTGCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGACCGAAACGCTGCGCCGGGAGGGTGAAGAACTGCGGCAGGCCAGCGAGGCAGAt                                                                                                                               <  1:255021/149‑1 (MQ=11)
                   cccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtct                                                                                                                             >  7:128210/1‑149 (MQ=21)
                    ccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGCCTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                            <  5:25067/149‑1 (MQ=255)
                    ccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAACAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                            >  3:288580/1‑149 (MQ=255)
                    ccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                            >  2:165392/1‑149 (MQ=21)
                    ccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                            >  4:159514/1‑149 (MQ=21)
                    ccGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtctc                                                                                                                            >  6:239953/1‑149 (MQ=21)
                      gTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGAtctc                                                                                                                            >  6:142423/1‑147 (MQ=39)
                      gTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTcca                                                                                                                          <  3:117257/149‑1 (MQ=21)
                          tGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAATGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTccagc                                                                                                                        >  3:239184/1‑147 (MQ=255)
                            cGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTccagcca                                                                                                                      <  6:49262/147‑1 (MQ=21)
                            cGAGGCGGGGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGGCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTccagcca                                                                                                                      <  1:396052/147‑1 (MQ=255)
                              aGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAggaa                                                                                                                  <  5:18800/149‑3 (MQ=19)
                              aGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAggaa                                                                                                                  <  7:27340/149‑3 (MQ=19)
                                 cGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTa                                                                                                               <  2:383441/149‑1 (MQ=255)
                                 cGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTa                                                                                                               <  5:141394/149‑1 (MQ=255)
                                  ggTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCgagg                                                                                                                                    >  4:262337/1‑126 (MQ=25)
                                  ggTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAt                                                                                                              <  1:130952/149‑1 (MQ=255)
                                  ggTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAt                                                                                                              >  3:35035/1‑149 (MQ=255)
                                   gTGGCAAGGGTTATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                             <  2:59387/149‑1 (MQ=255)
                                   gTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                             <  2:219563/149‑1 (MQ=255)
                                   gTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                             <  7:68591/149‑1 (MQ=255)
                                   gTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                             <  7:106261/149‑1 (MQ=255)
                                   gTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                             <  3:147721/149‑1 (MQ=255)
                                   gTGGCAAGGGTAAGGAGGTGCTTTATGACTCTGCCGCCGGCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                             <  4:84719/149‑1 (MQ=11)
                                     ggCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                             >  3:192573/1‑147 (MQ=255)
                                      gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                           <  5:8573/148‑1 (MQ=255)
                                      gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                           <  4:354/148‑1 (MQ=255)
                                      gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                           <  1:179833/148‑1 (MQ=255)
                                      gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                           <  8:393279/148‑1 (MQ=255)
                                      gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAg                                                                                                          <  7:206804/149‑1 (MQ=255)
                                      gCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATCGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGa                                                                                                           <  7:40400/148‑1 (MQ=255)
                                       gaaGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGAATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTTAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGt                                                                                                         <  1:156512/148‑1 (MQ=11)
                                        aaGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTa                                                                                                        >  7:336248/1‑149 (MQ=255)
                                        aaGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCAAAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTa                                                                                                        >  5:280380/1‑149 (MQ=255)
                                         aGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAATGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAc                                                                                                       <  4:140114/149‑1 (MQ=255)
                                         aGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTccagccag                                                                                                                     >  4:230448/1‑135 (MQ=21)
                                         aGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAc                                                                                                       >  1:68578/1‑149 (MQ=255)
                                          gggTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAc                                                                                                       >  8:332925/1‑148 (MQ=255)
                                             tAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAAc                                                                                                   <  4:182592/149‑1 (MQ=255)
                                             tAATGAGGAGCTTGATGACTCTGCCGCCGTCATAAAATGGTATGCCTAAAGGGCTGCTTAAATTGAGAACGAAAAGCTGAGCAGGTAGGTTCAAGCACTGCGGCAGGCCCGCGAGTCAGATATCCAGACAGGAACTATTGAGTACGAAc                                                                                                   >  8:143244/1‑149 (MQ=11)
                                               aTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAt                                                                                                                               >  5:319253/1‑119 (MQ=255)
                                               aTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGc                                                                                                 <  3:214597/149‑1 (MQ=255)
                                               aTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGc                                                                                                 >  1:313611/1‑149 (MQ=255)
                                                tGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGcc                                                                                                <  2:357000/149‑1 (MQ=255)
                                                  aGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTCGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCAt                                                                                              >  8:279233/1‑149 (MQ=255)
                                                   ggTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                             >  2:296404/1‑133 (MQ=255)
                                                   ggTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATc                                                                                             >  8:190388/1‑149 (MQ=255)
                                                    gTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATc                                                                                             <  8:120988/148‑1 (MQ=255)
                                                     tGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTg                                                                                                            <  4:20826/132‑1 (MQ=255)
                                                       cTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGAc                                                                                          <  7:304962/148‑1 (MQ=255)
                                                       cTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACt                                                                                         <  6:270823/149‑1 (MQ=255)
                                                       cTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACt                                                                                         >  7:94960/1‑149 (MQ=255)
                                                        tttATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAtt                                                                                                             >  4:10824/1‑128 (MQ=255)
                                                        tttATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACt                                                                                         >  7:23840/1‑148 (MQ=255)
                                                          tATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAc                                                                                      >  1:100595/1‑149 (MQ=255)
                                                           aTGACTCTGCCGCCGTCATAAAATGGTATGCCGACAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAc                                                                                      <  4:3827/148‑1 (MQ=255)
                                                           aTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGCACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAcg                                                                                     >  8:17004/1‑149 (MQ=255)
                                                           aTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGATGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAc                                                                                      <  5:114519/148‑1 (MQ=255)
                                                           aTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAcg                                                                                     >  1:23197/1‑149 (MQ=255)
                                                                tatGCCTCCGTCATAAAATGGTATGCCGAACCGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTgc                                                                                <  2:322664/147‑1 (MQ=11)
                                                                 ctGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGCTCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTgcg                                                                               >  4:63091/1‑149 (MQ=255)
                                                                  tGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGATATTGAGAACGAAAAGCTGCGCCGGGAGGGTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTgc                                                                                <  2:191638/147‑1 (MQ=11)
                                                                   gccgccGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTgcg                                                                               >  6:199582/1‑147 (MQ=255)
                                                                   gccgccGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCAAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTgcg                                                                               >  8:30273/1‑147 (MQ=255)
                                                                   gccgccGTCATAAAATGGTATGCCGAAAGGGATGCTGAAAGTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCa                                                                             <  6:306177/149‑1 (MQ=255)
                                                                    ccgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTgcg                                                                               <  3:264209/146‑1 (MQ=255)
                                                                    ccgccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAg                                                                            >  6:371000/1‑149 (MQ=255)
                                                                      gccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGc                                                                          <  3:168951/149‑1 (MQ=255)
                                                                       ccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAtc                                                                                                                              <  6:7510/96‑1 (MQ=255)
                                                                       ccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGcc                                                                         >  4:325647/1‑149 (MQ=255)
                                                                       ccgTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGcc                                                                         >  8:369554/1‑149 (MQ=255)
                                                                          tCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGac                                                                      >  5:44974/1‑148 (MQ=12)
                                                                          tCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGac                                                                      >  1:201728/1‑148 (MQ=12)
                                                                            aTAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGacg                                                                     >  5:158726/1‑146 (MQ=2)
                                                                            aTAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGacg                                                                     >  3:311906/1‑146 (MQ=2)
                                                                             tAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGAc                                                                                          <  6:111290/126‑1 (MQ=12)
                                                                              aaaaTGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAc                                                                                      >  4:290820/1‑129 (MQ=12)
                                                                                                      tGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCg                                                                                            <  3:370382/99‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCT  >  NC_000913/580896‑581186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 1432 total aligned reads displayed.
AGAACTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTG  >  NC_000913/580892‑581187
                                                                                                                                                      |                                                                                                                                                 
AGAACTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCG                                                                                                                                                   <  NB501016:230:HFVJLAFXY:1:21307:4899:4616/151‑1 (MQ=30)
 GAACTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGG                                                                                                                                                  <  NB501016:230:HFVJLAFXY:1:11308:18156:16303/151‑1 (MQ=28)
  AACTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGCTGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGC                                                                                                                                                 <  NB501016:230:HFVJLAFXY:1:21104:9694:14724/151‑1 (MQ=25)
  AACTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGC                                                                                                                                                 <  NB501016:230:HFVJLAFXY:4:11407:8246:17776/151‑1 (MQ=25)
  AACTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGC                                                                                                                                                 <  NB501016:230:HFVJLAFXY:3:11601:6426:14561/151‑1 (MQ=25)
      GGCAGGAACAGGGAATGCCCGTTCTTCGAGGAGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTGATAAAATGGTATGACGAAAGGGATGCTGAAATTGAGAACTAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAG                                                                                                                                               <  NB501016:230:HFVJLAFXY:4:11510:6860:7705/149‑1 (MQ=6)
      GGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAACGCTGCGCCGGGAGGTTGAAGCACTGCGGCAGGC                                                                                                                                             >  NB501016:230:HFVJLAFXY:2:11204:14172:10267/1‑151 (MQ=20)
      GGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGC                                                                                                                                             >  NB501016:230:HFVJLAFXY:3:11410:7865:18429/1‑151 (MQ=21)
      GGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGC                                                                                                                                             >  NB501016:230:HFVJLAFXY:3:21608:11680:18266/1‑151 (MQ=21)
      GGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGC                                                                                                                                             >  NB501016:230:HFVJLAFXY:2:11109:2711:7972/1‑151 (MQ=21)
      GGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGC                                                                                                                                             >  NB501016:230:HFVJLAFXY:2:11307:2209:10062/1‑151 (MQ=21)
      GGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGG                                                                                                                                              <  NB501016:230:HFVJLAFXY:4:21409:22814:12564/150‑1 (MQ=21)
      GGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGG                                                                                                                                              <  NB501016:230:HFVJLAFXY:1:11310:2066:11125/150‑1 (MQ=21)
      GGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGG                                                                                                                                              <  NB501016:230:HFVJLAFXY:4:21506:1235:16895/150‑1 (MQ=21)
      GGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAG                                                                                                                                               <  NB501016:230:HFVJLAFXY:4:21609:11847:12521/149‑1 (MQ=21)
       GCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCC                                                                                                                                            <  NB501016:230:HFVJLAFXY:4:11503:9241:11933/151‑1 (MQ=21)
       GCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCC                                                                                                                                            <  NB501016:230:HFVJLAFXY:3:11402:7207:16831/151‑1 (MQ=21)
        CAGGGACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCC                                                                                                                                            >  NB501016:230:HFVJLAFXY:4:11611:11738:11700/1‑150 (MQ=20)
        CAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCC                                                                                                                                            >  NB501016:230:HFVJLAFXY:1:11103:6926:5943/1‑150 (MQ=21)
          GGAACAGGGAATGCCCGTTCTGTGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGTTTGAAGAACTGCGGCAGGCCAGC                                                                                                                                         >  NB501016:230:HFVJLAFXY:2:11201:2144:13503/1‑151 (MQ=20)
           GAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCG                                                                                                                                        >  NB501016:230:HFVJLAFXY:2:21310:13112:11448/1‑151 (MQ=21)
           GAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGACGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCG                                                                                                                                        >  NB501016:230:HFVJLAFXY:4:11406:11639:9223/1‑151 (MQ=20)
            AACAGGGAATGCCCGTTCTGCGAGGCGGTGTCAAGGGTTATGAGGTGCTTTATGTCTCTTCCGCAGTCATAAAATTGTATGCCGAAAGGGATGCTGAAATGGAGAACGAAAAGCTGGGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGA                                                                                                                                       <  NB501016:230:HFVJLAFXY:1:21207:6552:8364/151‑1 (MQ=17)
            AACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGA                                                                                                                                       <  NB501016:230:HFVJLAFXY:4:21403:14294:11100/151‑1 (MQ=21)
             CCAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCGTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAG                                                                                                                                      <  NB501016:230:HFVJLAFXY:3:11610:15921:2090/151‑1 (MQ=20)
             ACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAG                                                                                                                                      <  NB501016:230:HFVJLAFXY:4:11505:12922:11788/151‑1 (MQ=21)
              CAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGG                                                                                                                                     >  NB501016:230:HFVJLAFXY:1:11205:22180:16529/1‑151 (MQ=21)
              CAGGGAATGCCCGTTCTGCGAGGCGGGGGCAAGGGTAATGAGGTTCTTTAAGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGG                                                                                                                                     <  NB501016:230:HFVJLAFXY:4:21608:1094:3788/151‑1 (MQ=19)
              CAGGGAAGGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCGTTATGACTCGGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCGGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGG                                                                                                                                     <  NB501016:230:HFVJLAFXY:3:11403:26182:14747/151‑1 (MQ=19)
               CGGGAATGCCCGTGCTGCGAGGCGGGGGCAAGGGTAATGAGGTGCTGTCTGACGCTGCCGCCGTCATAAAATGGTATGCCGAACGGGAGGCTGAAATTGAGACCGAAAAGCTGCGCCGGGAGGTGGAAGACCTGCGGCAGGCCAGCGAGGC                                                                                                                                    <  NB501016:230:HFVJLAFXY:1:11105:4558:1279/151‑1 (MQ=16)
               AGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTTAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11606:1273:13948/151‑1 (MQ=20)
               AGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    <  NB501016:230:HFVJLAFXY:1:11303:17630:7379/151‑1 (MQ=21)
               AGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    <  NB501016:230:HFVJLAFXY:3:21408:8005:10485/151‑1 (MQ=21)
                GGGAATGCCCGTTCTGCGATGCGGTGGCAAGGGTAATGAGGTGCATTATCCCTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCCCCGGGATGTTGAAGAACTGCGTCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:1:11307:15980:15353/1‑150 (MQ=6)
                GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGCACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:4:11511:2917:15854/1‑150 (MQ=20)
                GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:2:11211:8390:11389/1‑150 (MQ=21)
                GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:4:21606:24885:1698/1‑150 (MQ=21)
                GGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCA                                                                                                                                                <  NB501016:230:HFVJLAFXY:3:21612:3304:11310/138‑1 (MQ=24)
                 GGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAG                                                                                                                                  <  NB501016:230:HFVJLAFXY:1:21204:4861:16708/151‑1 (MQ=21)
                 GGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAG                                                                                                                                  <  NB501016:230:HFVJLAFXY:4:11509:6055:3041/151‑1 (MQ=21)
                  GAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGG                                                                                                                                              <  NB501016:230:HFVJLAFXY:4:21505:10573:2987/138‑1 (MQ=22)
                   AATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCAT‑AAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:2:11205:19822:1831/1‑146 (MQ=7)
                    ATGCCCGTGCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGACCGAAACGCTGCGCCGGGAGGGTGAAGAACTGCGGCAGGCCAGCGAGGCAGATC                                                                                                                               <  NB501016:230:HFVJLAFXY:1:21110:3870:7591/151‑1 (MQ=18)
                      GCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTC                                                                                                                             >  NB501016:230:HFVJLAFXY:1:11102:14846:8925/1‑151 (MQ=20)
                      GCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGG                                                                                                                                              >  NB501016:230:HFVJLAFXY:4:11403:23816:4071/1‑134 (MQ=22)
                       CCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGGTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    <  NB501016:230:HFVJLAFXY:3:21604:24400:2553/143‑1 (MQ=21)
                       CCCGTTCTGCGAGGCGGTGGCAAGGGTAACGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:1:11211:17401:4376/1‑143 (MQ=21)
                       CCCGTTCTGCGAGGCGGGGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCC                                                                                                                            >  NB501016:230:HFVJLAFXY:3:21611:9614:3246/1‑151 (MQ=20)
                        CCGTTCTGCGAGGCGGTGGCAAGGGTCAGGAGGCGCTTTATGACTCTGCCGCCGTCATAAAATGGTCTGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCA                                                                                                                           <  NB501016:230:HFVJLAFXY:3:21506:11824:5138/151‑1 (MQ=18)
                         CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:4:11411:25511:12994/151‑1 (MQ=20)
                         CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:1:21309:16358:3673/151‑1 (MQ=20)
                         CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:4:21511:23598:3576/151‑1 (MQ=20)
                         CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCAGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:2:21308:23349:4866/151‑1 (MQ=20)
                         CGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGGGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGACCTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:2:11112:25024:20148/151‑1 (MQ=19)
                         CGGTCTGCGAGGCTGTGGCAAGGGTAATGAGGTGCTTTATGACTCGGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGGGGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:3:21411:4668:20365/151‑1 (MQ=18)
                          GTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGC                                                                                                                         <  NB501016:230:HFVJLAFXY:1:11210:13423:11376/151‑1 (MQ=20)
                             CTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCC                                                                                                                        >  NB501016:230:HFVJLAFXY:2:21102:11206:12337/1‑149 (MQ=20)
                             CTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCC                                                                                                                        >  NB501016:230:HFVJLAFXY:4:21410:25865:9343/1‑149 (MQ=20)
                             CTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCC                                                                                                                        >  NB501016:230:HFVJLAFXY:2:11104:4916:11946/1‑149 (MQ=20)
                               GCTAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCGGAAATTGATGCTGAAATTGAGAACTATAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGA                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11509:15270:5409/151‑1 (MQ=17)
                               GCGAGGCGGTGGCAAGGGTCATGAGTTGCTTTCTGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGATAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:2:21209:13792:3158/150‑1 (MQ=18)
                               GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAACTGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:2:21212:24420:17740/150‑1 (MQ=20)
                               GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     >  NB501016:230:HFVJLAFXY:4:11409:7448:10454/1‑150 (MQ=20)
                               GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     >  NB501016:230:HFVJLAFXY:1:11203:21738:16296/1‑150 (MQ=20)
                               GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:3:11408:19885:12361/150‑1 (MQ=20)
                               GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:4:11602:17692:2674/150‑1 (MQ=20)
                               GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAG                                                                                                                      <  NB501016:230:HFVJLAFXY:2:11306:12903:5995/149‑1 (MQ=20)
                               GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTC                                                                                                                             <  NB501016:230:HFVJLAFXY:3:21605:7470:4589/142‑1 (MQ=21)
                               GCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGAGCTCCAGCCAGG                                                                                                                     <  NB501016:230:HFVJLAFXY:3:11511:8154:12874/150‑1 (MQ=20)
                                 GAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  <  NB501016:230:HFVJLAFXY:2:21205:5292:17503/151‑1 (MQ=20)
                                 GAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  <  NB501016:230:HFVJLAFXY:3:11510:22650:16453/151‑1 (MQ=20)
                                 GAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  <  NB501016:230:HFVJLAFXY:2:21105:20142:12044/151‑1 (MQ=20)
                                 GAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGGCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  <  NB501016:230:HFVJLAFXY:2:11106:26655:12294/151‑1 (MQ=20)
                                   GGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTA                                                                                                                <  NB501016:230:HFVJLAFXY:1:21102:13746:9156/151‑1 (MQ=20)
                                    GCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGTAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAT                                                                                                               <  NB501016:230:HFVJLAFXY:1:11201:26761:13728/151‑1 (MQ=19)
                                    GCGGTGGCAAGGGTAATGAGGTGCTTTAAGACTCTGCCGCCGGCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTAT                                                                                                               <  NB501016:230:HFVJLAFXY:3:21602:15068:13892/151‑1 (MQ=19)
                                     CGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATT                                                                                                              <  NB501016:230:HFVJLAFXY:1:21201:1491:8251/151‑1 (MQ=20)
                                     CGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATT                                                                                                              <  NB501016:230:HFVJLAFXY:2:11202:2070:8390/151‑1 (MQ=20)
                                     CGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATT                                                                                                              <  NB501016:230:HFVJLAFXY:3:21512:8559:14603/151‑1 (MQ=20)
                                     CGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATT                                                                                                              <  NB501016:230:HFVJLAFXY:1:11110:22122:7426/151‑1 (MQ=20)
                                      gggtGCAAGGGTAATGAGGGGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCGGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGCAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:2:21102:7228:3361/147‑1 (MQ=18)
                                      GGTGGGAAGGGTAATGAGGGTCTTTATGACTCTGCCGACGTCATAAAATGGTATGCCGAAATGGATGCTGAAATTGAGATCGAAATGCTGCGCCTGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:3:11604:23630:2587/151‑1 (MQ=16)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTTCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:4:11404:4389:1041/151‑1 (MQ=19)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCGGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTCGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:3:21410:15648:8648/151‑1 (MQ=19)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAATGGGATGCTGAAATTGAGAACGAAAAGCTGCGCAGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:4:21507:6907:14991/151‑1 (MQ=19)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:2:11211:15144:14827/151‑1 (MQ=20)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:3:11504:3029:12379/151‑1 (MQ=20)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             >  NB501016:230:HFVJLAFXY:1:11212:2369:10884/1‑151 (MQ=20)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:2:21209:8066:13933/151‑1 (MQ=20)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:1:11104:16021:2273/151‑1 (MQ=20)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:1:11107:19707:10195/151‑1 (MQ=20)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             >  NB501016:230:HFVJLAFXY:2:11312:4089:5681/1‑151 (MQ=20)
                                      GGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCACCAGCCAGGAACTATTG                                                                                                             >  NB501016:230:HFVJLAFXY:3:11404:23057:18461/1‑151 (MQ=19)
                                       GTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGA                                                                                                            <  NB501016:230:HFVJLAFXY:4:21402:16388:15750/151‑1 (MQ=20)
                                       GTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             >  NB501016:230:HFVJLAFXY:3:11505:9100:4074/1‑150 (MQ=20)
                                        TGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             >  NB501016:230:HFVJLAFXY:1:21310:17223:5875/1‑149 (MQ=20)
                                         TGCAAGGGTAATGAGGTGCTTTCTGACTCTCCCGCCGTCATAAAATGGTATGCCGAAAGTGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:3:21504:25929:19576/150‑1 (MQ=18)
                                         GGCAAGTGTAATGAGTTGCTTTTTGAGTCTGCCGCCGTCATAAATTTGTATGCCGAAAGGTAGGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAAGTGCGGCTGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:1:11302:13937:9811/150‑1 (MQ=15)
                                         GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTTAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:1:11108:25760:10688/150‑1 (MQ=19)
                                         GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGT                                                                                                          >  NB501016:230:HFVJLAFXY:4:21611:18488:15826/1‑151 (MQ=20)
                                         GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:1:11309:16059:8842/150‑1 (MQ=20)
                                         GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:1:21109:3054:9144/150‑1 (MQ=20)
                                         GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:4:11409:1527:8482/150‑1 (MQ=20)
                                         GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:2:11104:10185:11153/150‑1 (MQ=20)
                                         GGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:3:21403:12410:17953/150‑1 (MQ=20)
                                         GGCAAGGGTAATGAGGTGCTTTATGACTCTGCAGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:3:21603:4175:8503/150‑1 (MQ=19)
                                          ggatGGGTAATGAGGTGCTTTATGACTCTGCCGCAGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGATAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTA                                                                                                         <  NB501016:230:HFVJLAFXY:3:11506:26694:15559/147‑1 (MQ=19)
                                          GCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTA                                                                                                         <  NB501016:230:HFVJLAFXY:1:11101:20327:7737/151‑1 (MQ=20)
                                          GCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTA                                                                                                         <  NB501016:230:HFVJLAFXY:4:21601:14202:19321/151‑1 (MQ=20)
                                          GCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTA                                                                                                         <  NB501016:230:HFVJLAFXY:4:11603:13395:5942/151‑1 (MQ=20)
                                          GCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTA                                                                                                         <  NB501016:230:HFVJLAFXY:1:21202:3049:17081/151‑1 (MQ=20)
                                          GCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTA                                                                                                         <  NB501016:230:HFVJLAFXY:3:11406:16512:8648/151‑1 (MQ=20)
                                           CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATTGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAC                                                                                                        <  NB501016:230:HFVJLAFXY:1:11304:26707:4046/151‑1 (MQ=19)
                                           CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAC                                                                                                        >  NB501016:230:HFVJLAFXY:4:11604:12141:10904/1‑151 (MQ=20)
                                           CAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAG                                                                                                                          <  NB501016:230:HFVJLAFXY:2:21207:5022:13862/133‑1 (MQ=21)
                                            AAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       <  NB501016:230:HFVJLAFXY:1:21108:1278:7132/151‑1 (MQ=20)
                                            AAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       <  NB501016:230:HFVJLAFXY:4:11610:12511:5305/151‑1 (MQ=20)
                                             AGGGTAATGAGGTGCTTTATGACTCTGGCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATATAGAACGAAAAGATGCGCCGGGAGGTTGAAGAAATGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTAtc                                                                                                       >  NB501016:230:HFVJLAFXY:4:11608:11796:6224/1‑148 (MQ=17)
                                               GGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAAC                                                                                                    <  NB501016:230:HFVJLAFXY:3:11502:6386:18302/151‑1 (MQ=20)
                                                GTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACG                                                                                                   <  NB501016:230:HFVJLAFXY:2:21310:9297:9325/151‑1 (MQ=20)
                                                GTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACG                                                                                                   <  NB501016:230:HFVJLAFXY:3:21507:11616:8552/151‑1 (MQ=20)
                                                GTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCA                                                                                                                           <  NB501016:230:HFVJLAFXY:3:21503:13659:4036/127‑1 (MQ=22)
                                                GTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGC                                                                                                                                                 <  NB501016:230:HFVJLAFXY:2:21106:8065:12672/105‑1 (MQ=29)
                                                  AATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCC                                                                                                 >  NB501016:230:HFVJLAFXY:1:21209:11815:7643/1‑151 (MQ=20)
                                                  AATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCC                                                                                                 >  NB501016:230:HFVJLAFXY:3:11608:21245:4624/1‑151 (MQ=20)
                                                  AATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGC                                                                                                  <  NB501016:230:HFVJLAFXY:1:21107:15376:2726/150‑1 (MQ=20)
                                                   ATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGCTCTCCAGCCAGGAACTATTGAGTACGAACGCC                                                                                                 >  NB501016:230:HFVJLAFXY:2:11209:7419:6103/1‑150 (MQ=19)
                                                   ATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCC                                                                                                 >  NB501016:230:HFVJLAFXY:2:21301:6604:7490/1‑150 (MQ=20)
                                                    TGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCAT                                                                                               >  NB501016:230:HFVJLAFXY:2:21312:13992:1819/1‑151 (MQ=20)
                                                    TGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAG                                                                                                                                  >  NB501016:230:HFVJLAFXY:2:11304:5144:5476/1‑116 (MQ=23)
                                                     GAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCTGGCCAGCGAGGCAGATCTCCAGCCAGGAACTA                                                                                                                >  NB501016:230:HFVJLAFXY:4:11501:26177:1493/1‑133 (MQ=20)
                                                     GAGGTGCTTTATGACTCTGCCGCCGTAATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCAT                                                                                               <  NB501016:230:HFVJLAFXY:3:11603:26679:18150/150‑1 (MQ=7)
                                                      AGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGCACGCCATCG                                                                                             >  NB501016:230:HFVJLAFXY:2:21203:7685:9857/1‑151 (MQ=19)
                                                      AGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCG                                                                                             >  NB501016:230:HFVJLAFXY:1:21302:13100:18555/1‑151 (MQ=20)
                                                       GGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGC                                                                                            >  NB501016:230:HFVJLAFXY:1:21212:4587:7406/1‑151 (MQ=20)
                                                       GGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCAGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACG                                                                                                   >  NB501016:230:HFVJLAFXY:2:11111:19564:3403/1‑144 (MQ=20)
                                                       GGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGAGGCTGAATTTGAGAACGCAAAGCTGCGCCGGGAGGTTGATGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCG                                                                                             >  NB501016:230:HFVJLAFXY:1:11104:3531:10168/1‑150 (MQ=18)
                                                        ccattttatgacggcggcagaGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGCC                                                                                           >  NB501016:230:HFVJLAFXY:3:11409:22067:16467/22‑151 (MQ=16)
                                                        GTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAGGAACTGCGGCAGGCCAGGGAGGCAGATGTCCAGCCAGGACCTATTGAGTCCGACCGCCATCGCC                                                                                           >  NB501016:230:HFVJLAFXY:1:11312:4754:14641/1‑151 (MQ=17)
                                                         cattttatgacggcggcagaGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACT                                                                                          >  NB501016:230:HFVJLAFXY:2:21305:15404:20036/21‑151 (MQ=17)
                                                         TGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATC                                                                                              >  NB501016:230:HFVJLAFXY:1:21306:26453:4303/1‑147 (MQ=20)
                                                          GCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTT                                                                                         >  NB501016:230:HFVJLAFXY:2:11306:7935:19131/1‑151 (MQ=20)
                                                          GCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACT                                                                                          <  NB501016:230:HFVJLAFXY:2:11302:25229:11152/150‑1 (MQ=20)
                                                          GCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACT                                                                                          <  NB501016:230:HFVJLAFXY:1:21311:22029:1338/150‑1 (MQ=20)
                                                           CTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTT                                                                                         >  NB501016:230:HFVJLAFXY:4:11609:13842:16674/1‑150 (MQ=20)
                                                           CTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTT                                                                                         >  NB501016:230:HFVJLAFXY:3:21609:17395:13512/1‑150 (MQ=20)
                                                            TTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGATAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAC                                                                                       >  NB501016:230:HFVJLAFXY:4:11412:23714:16447/1‑151 (MQ=19)
                                                             TTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAC                                                                                       <  NB501016:230:HFVJLAFXY:3:11507:22097:6317/150‑1 (MQ=20)
                                                              TATGACTCTGCCTCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACG                                                                                      <  NB501016:230:HFVJLAFXY:1:11306:11239:5945/150‑1 (MQ=19)
                                                              TATGACTCTGCCGCCGTCATAAACTGGTATTCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACG                                                                                      <  NB501016:230:HFVJLAFXY:1:11310:7420:12623/150‑1 (MQ=19)
                                                              TATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTTAGTACGAACGCCATCGACTTACG                                                                                      <  NB501016:230:HFVJLAFXY:3:21606:5203:10684/150‑1 (MQ=19)
                                                              TATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACG                                                                                      <  NB501016:230:HFVJLAFXY:3:11602:22508:6656/150‑1 (MQ=20)
                                                              TATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCAATCGACTTACTC                                                                                     >  NB501016:230:HFVJLAFXY:1:21211:26310:2695/1‑151 (MQ=19)
                                                               ATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCG                                                                                    >  NB501016:230:HFVJLAFXY:4:21405:8723:10077/1‑151 (MQ=20)
                                                               ATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCG                                                                                    <  NB501016:230:HFVJLAFXY:1:21303:21567:11905/151‑1 (MQ=20)
                                                                TGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGCAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             >  NB501016:230:HFVJLAFXY:4:11606:4171:2636/1‑125 (MQ=20)
                                                                TGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGT                                                                                   >  NB501016:230:HFVJLAFXY:3:11611:7578:5223/1‑151 (MQ=20)
                                                                TGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTG                                                                                                             <  NB501016:230:HFVJLAFXY:2:21110:1541:16421/125‑1 (MQ=21)
                                                                 GACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTG                                                                                  <  NB501016:230:HFVJLAFXY:1:21206:7291:10598/151‑1 (MQ=20)
                                                                 GACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCA                                                                                                >  NB501016:230:HFVJLAFXY:3:21510:16140:9499/1‑137 (MQ=20)
                                                                   CTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCG                                                                                >  NB501016:230:HFVJLAFXY:4:21412:13169:5841/1‑151 (MQ=20)
                                                                   CTCTGCCGCCGTCATAAAAAGGGATGCGGAAACGGAAGATGAAATTTAGAACAAAAACCTGACGCGGGAGGGTAAAGAACTGCTGCAGTCCATCGAGGCAGATCTCCAACCAGGAAATATTGATTACTAACGCCATCGACatcagctttc                                                                                 >  NB501016:230:HFVJLAFXY:4:21603:12590:16870/1‑140 (MQ=12)
                                                                    TCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGC                                                                               >  NB501016:230:HFVJLAFXY:4:21502:24257:8559/1‑151 (MQ=20)
                                                                    TCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTGGAAGAACTGCGGCCGGCCAGCGAGGCAGATCTCCAGCCAGGAGCTATTGAGTACTAACGCCATCGACTTACGCGTGCGC                                                                               >  NB501016:230:HFVJLAFXY:2:21111:24284:3658/1‑151 (MQ=18)
                                                                     CTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGC                                                                               >  NB501016:230:HFVJLAFXY:2:11207:3198:14944/1‑150 (MQ=20)
                                                                     CTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCG                                                                                <  NB501016:230:HFVJLAFXY:3:11401:5245:3922/149‑1 (MQ=20)
                                                                     CTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACT                                                                                          <  NB501016:230:HFVJLAFXY:4:21509:6409:3267/139‑1 (MQ=20)
                                                                      TGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGC                                                                               >  NB501016:230:HFVJLAFXY:4:11509:12260:10023/1‑149 (MQ=20)
                                                                       GCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCG                                                                                                                                                   >  NB501016:230:HFVJLAFXY:3:21406:22984:6720/1‑80 (MQ=39)
                                                                         CGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCC                                                                          <  NB501016:230:HFVJLAFXY:2:21211:4034:7591/151‑1 (MQ=20)
                                                                         CGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCC                                                                          >  NB501016:230:HFVJLAFXY:2:21103:23209:11465/1‑151 (MQ=20)
                                                                         CGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCG                                                                                             >  NB501016:230:HFVJLAFXY:2:21108:5067:8511/1‑132 (MQ=21)
                                                                          GCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCG                                                                         <  NB501016:230:HFVJLAFXY:4:11601:12207:7814/151‑1 (MQ=20)
                                                                          GCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCG                                                                         >  NB501016:230:HFVJLAFXY:2:11310:21160:6640/1‑151 (MQ=20)
                                                                           CCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCG                                                                         >  NB501016:230:HFVJLAFXY:4:21604:21547:13258/1‑150 (MQ=20)
                                                                           CCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCG                                                                         >  NB501016:230:HFVJLAFXY:4:11401:2784:4877/1‑150 (MQ=20)
                                                                             GTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCTCCGGGAGGTTGAAGAACAGCGGAAGGCCAGCGAGGCAGATCTCCAGCCAGGAACAATAGAGTACGAACGCCAACGACTTACGCGaccgaagcccgacc                                                                      >  NB501016:230:HFVJLAFXY:3:11605:25683:13837/1‑137 (MQ=14)
                                                                             GTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACG                                                                      >  NB501016:230:HFVJLAFXY:1:21210:19669:10429/1‑151 (MQ=9)
                                                                             GTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACG                                                                      <  NB501016:230:HFVJLAFXY:1:11112:19497:13026/151‑1 (MQ=9)
                                                                             GTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCAT                                                                                               <  NB501016:230:HFVJLAFXY:2:11102:14413:8002/126‑1 (MQ=17)
                                                                              TCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGC                                                                     >  NB501016:230:HFVJLAFXY:3:11606:26274:6502/1‑151 (MQ=5)
                                                                              TCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTG                                                                                  >  NB501016:230:HFVJLAFXY:1:11207:7198:13065/1‑138 (MQ=11)
                                                                              TCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACG                                                                                                       >  NB501016:230:HFVJLAFXY:3:21404:17611:2063/1‑117 (MQ=12)
                                                                               CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGACGACGC                                                                     >  NB501016:230:HFVJLAFXY:2:11101:1697:12734/1‑150 (MQ=28)
                                                                               CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGccgc                                                                     >  NB501016:230:HFVJLAFXY:3:11405:19021:16357/1‑146 (MQ=9)
                                                                               CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGAcgcc                                                                    >  NB501016:230:HFVJLAFXY:1:21103:21769:9296/1‑147 (MQ=3)
                                                                               CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGC                                                                     >  NB501016:230:HFVJLAFXY:2:11309:20532:15478/1‑150 (MQ=3)
                                                                               CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGC                                                                     >  NB501016:230:HFVJLAFXY:3:21508:24172:8726/1‑150 (MQ=3)
                                                                               CATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGC                                                                     >  NB501016:230:HFVJLAFXY:4:21401:16413:14879/1‑150 (MQ=3)
                                                                                ATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAGCGCCATCGCCTTACGCGTGCGCAGGCCGAcgccc                                                                   >  NB501016:230:HFVJLAFXY:2:21112:25102:11004/1‑146 (MQ=1)
                                                                                ATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGCAC                                                                   >  NB501016:230:HFVJLAFXY:2:21303:25959:12764/1‑151 (MQ=0)
                                                                                ATAAAATGGTAGGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCAGGGAGGATGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGAAGCAC                                                                   >  NB501016:230:HFVJLAFXY:2:11105:25134:3421/1‑151 (MQ=6)
                                                                                 TAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAG                                                                                                           <  NB501016:230:HFVJLAFXY:4:11507:18466:17405/110‑1 (MQ=6)
                                                                                 TAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAAC                                                                                                                  >  NB501016:230:HFVJLAFXY:2:21312:7106:2661/1‑103 (MQ=6)
                                                                                  AAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTG                                                                                  <  NB501016:230:HFVJLAFXY:2:11103:18134:13905/134‑1 (MQ=3)
                                                                                   AAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGAC                                                                       >  NB501016:230:HFVJLAFXY:2:21202:15668:9679/1‑144 (MQ=0)
                                                                                          ATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGtcggcctgcgcacgcgtaagtc                                                          <  NB501016:230:HFVJLAFXY:2:11108:17364:15660/150‑23 (MQ=0)
                                                                                             CCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGG                                                                            >  NB501016:230:HFVJLAFXY:3:21401:10674:16829/1‑129 (MQ=0)
                                                                                                         CTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTAagatcggaagagcacagtctgaactccagtcaccggctatgatgtc                                          >  NB501016:230:HFVJLAFXY:4:21407:2780:16107/1‑105 (MQ=0)
                                                                                                               TTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCG                                                                         >  NB501016:230:HFVJLAFXY:4:11611:9479:3074/1‑114 (MQ=0)
                                                                                                                                                      |                                                                                                                                                 
AGAACTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTG  >  NC_000913/580892‑581187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑