Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,636,068 T→C 100% pseudogene (300/528 nt) nohQ ← pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,636,0680TC99.0% 7112.4 / 19.1 3537pseudogene (300/528 nt)nohQpseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin
Reads supporting (aligned to +/- strand):  ref base T (19/19);  new base C (1699/1796);  total (1719/1818)
Fisher's exact test for biased strand distribution p-value = 8.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 3537 total aligned reads displayed.

TTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTT  >  NC_000913/1635935‑1636213
                                                                                                                                     |                                                                                                                                                 
   ccAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTc                                                                                                                                  <  5:208180/148‑1 (MQ=12)
    cAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGCATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCgg                                                                                                                                >  2:32412/1‑149 (MQ=17)
    cAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCg                                                                                                                                 <  7:282669/148‑1 (MQ=12)
          cGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACCGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTgcg                                                                                                                          >  5:285965/1‑149 (MQ=255)
          cGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTgcg                                                                                                                          >  2:25923/1‑149 (MQ=12)
           ggAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGTGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTgcg                                                                                                                          >  1:40892/1‑148 (MQ=255)
              aaaCGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCAcg                                                                                                                      <  5:178019/149‑1 (MQ=12)
               aaCGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCAcgc                                                                                                                     <  1:126354/149‑1 (MQ=12)
               aaCGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCAcgc                                                                                                                     <  2:208002/149‑1 (MQ=12)
                  gccgcTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGt                                                                                                                   >  2:177662/1‑148 (MQ=12)
                         cACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGa                                                                                                            <  5:126021/148‑1 (MQ=12)
                         cACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGAt                                                                                                           <  5:260256/149‑1 (MQ=12)
                            cGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGTGTCGGCCTGCGCACGCGTAAGTCGATGGc                                                                                                        <  7:137972/149‑1 (MQ=255)
                            cGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGc                                                                                                        <  3:69482/149‑1 (MQ=12)
                            cGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGc                                                                                                        <  6:159048/149‑1 (MQ=12)
                               cggggggAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCACTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGtt                                                                                                     <  2:348578/147‑1 (MQ=255)
                               cAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCCTTCTTCAGTTCCTGTGCGTCGGCCTGCGCCAGCGTAAGTCGCCGGCGtt                                                                                                     >  8:369760/1‑149 (MQ=11)
                                aGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTc                                                                                                    <  5:75824/149‑1 (MQ=12)
                                     gagcccGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACt                                                                                               <  4:319514/146‑1 (MQ=12)
                                     gagcccGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGAACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCTTAAGTCGATGGCGTTCGTACt                                                                                               <  1:279005/146‑1 (MQ=11)
                                        cccGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCg                                                                                                       >  4:252274/1‑138 (MQ=32)
                                        cccGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCaa                                                                                            >  8:344133/1‑149 (MQ=17)
                                        cccGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCa                                                                                             >  4:277550/1‑148 (MQ=17)
                                          cGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGTAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATa                                                                                          <  7:362701/149‑1 (MQ=12)
                                          cGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCTCACGCGTAAGTCGATGGCGTTCGTACTCaa                                                                                            >  8:229748/1‑147 (MQ=12)
                                               agaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTtcc                                                                                     <  3:98125/149‑1 (MQ=12)
                                               agaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTtcc                                                                                     >  5:101496/1‑149 (MQ=12)
                                               agaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTtcc                                                                                     <  3:143165/149‑1 (MQ=12)
                                               agaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCAGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTtcc                                                                                     >  2:148215/1‑149 (MQ=255)
                                                gaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCct                                                                                    >  7:189086/1‑149 (MQ=12)
                                                gaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCct                                                                                    <  6:208310/149‑1 (MQ=12)
                                                gaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACAACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCct                                                                                    >  2:116009/1‑149 (MQ=255)
                                                  aTACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCAGCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctgg                                                                                  <  6:232075/149‑1 (MQ=255)
                                                  aTACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACGACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctgg                                                                                  >  4:300487/1‑149 (MQ=255)
                                                   tACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggc                                                                                 >  2:47561/1‑149 (MQ=12)
                                                    aCTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggct                                                                                >  4:68781/1‑149 (MQ=12)
                                                      tGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTgc                                                                                                                           <  6:182407/104‑1 (MQ=12)
                                                      tGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATg                                                                                                          >  7:332522/1‑121 (MQ=17)
                                                      tGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGttc                                                                                      >  3:26491/1‑139 (MQ=12)
                                                      tGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctg                                                                               >  3:329179/1‑148 (MQ=12)
                                                      tGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctg                                                                               >  3:110578/1‑148 (MQ=12)
                                                        gCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGgag                                                                            <  8:197006/149‑1 (MQ=12)
                                                          aaTTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGAt                                                                          <  8:101500/149‑1 (MQ=12)
                                                          aaTTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGAt                                                                          <  5:369636/149‑1 (MQ=12)
                                                           aagtCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGTCATTCTTCAGTTCCTGTGCGTCTGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCTATAGTTCCTGGCTGGAGATc                                                                         <  4:42105/146‑1 (MQ=11)
                                                            tttCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTgcgc                                                                                                                         <  4:226612/100‑1 (MQ=16)
                                                            tttCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCt                                                                        <  6:317823/149‑1 (MQ=12)
                                                            tttCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCt                                                                        <  7:22115/149‑1 (MQ=12)
                                                             ttCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTg                                                                       >  4:193147/1‑149 (MQ=12)
                                                               cACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTgcc                                                                     >  6:133879/1‑147 (MQ=12)
                                                               cACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTgc                                                                      <  3:282932/148‑2 (MQ=12)
                                                               cACCTGCGATCCGCGACAGCAAGAAAGTACAGAATGCGGTTTCCACCACTTGAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTGGGCCTGCGCACGCGTAAGTGGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTgc                                                                      <  7:104904/148‑2 (MQ=11)
                                                                aCCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGcct                                                                    <  2:379528/149‑1 (MQ=255)
                                                                aCCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCGCTGGCAGTCTGCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGcct                                                                    <  8:253442/149‑1 (MQ=11)
                                                                  cTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCg                                                                  >  1:333085/1‑149 (MQ=255)
                                                                    gCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCt                                                                >  3:351695/1‑149 (MQ=255)
                                                                     cGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGc                                                                 <  4:343685/147‑1 (MQ=32)
                                                                     cGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTg                                                               <  2:231617/149‑1 (MQ=255)
                                                                          cgcgACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAGAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTg                                                          >  8:163848/1‑149 (MQ=11)
                                                                                  cACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAg                                                    <  1:199498/147‑1 (MQ=255)
                                                                                  cACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGtt                                                  >  7:308123/1‑149 (MQ=255)
                                                                                   aCGTAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCCCGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGAGCTGCCTCGCGGGCCTGCCGCAGttc                                                 >  8:288598/1‑149 (MQ=11)
                                                                                   aCGAAAGTACAGAATGCGGGTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCAcgc                                                                                                                     <  3:44010/81‑1 (MQ=11)
                                                                                     gAAAGTACAGCATGCGGTTTCCACCCCTTCAGCGGAGTCTCTGGCATTCGTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTACTGGCTCGAGATCTGCCTCGCTGGCCTGCCGCAGttctt                                               >  1:69347/1‑149 (MQ=11)
                                                                                     gAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttctt                                               >  8:314208/1‑149 (MQ=255)
                                                                                     gAAAGTACAGAATGCGGTTTCCACCACGTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTGCTGAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttctt                                               >  6:80377/1‑149 (MQ=11)
                                                                                      aaaGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttcttc                                              <  7:16258/149‑1 (MQ=255)
                                                                                         gTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAAc                                           <  1:91389/149‑1 (MQ=255)
                                                                                          tACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTCCTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACt                                          >  6:107820/1‑149 (MQ=25)
                                                                                                aTGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttcttc                                              >  6:26034/1‑139 (MQ=255)
                                                                                                 tGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGa                                                                                                            <  3:228392/76‑1 (MQ=255)
                                                                                                     gTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAg                               >  5:230600/1‑149 (MQ=11)
                                                                                                      tttCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGc                              <  7:49788/149‑1 (MQ=11)
                                                                                                          caccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCtttt                          <  4:165031/149‑1 (MQ=11)
                                                                                                          caccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCtttt                          >  8:71410/1‑149 (MQ=11)
                                                                                                          caccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCtttt                          >  2:78325/1‑149 (MQ=11)
                                                                                                           accacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTc                         >  1:160407/1‑149 (MQ=11)
                                                                                                            ccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCg                        >  7:253902/1‑149 (MQ=11)
                                                                                                            ccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCg                        <  1:100552/149‑1 (MQ=11)
                                                                                                               cTTCCGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTtc                     >  3:198499/1‑149 (MQ=11)
                                                                                                                ttCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTtct                    >  6:45581/1‑149 (MQ=11)
                                                                                                                ttCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTtct                    >  8:125149/1‑149 (MQ=11)
                                                                                                                  cAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCAAGCGTAAGTCGATGGCGTTCGTACTCACTAGTTCATGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTtct                    <  1:247340/147‑1 (MQ=11)
                                                                                                                      ggAGTCTCTGGCATTCTTCAGTTCCTGTGCGGCGGCCGGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTTGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAAttt              <  5:51773/149‑1 (MQ=11)
                                                                                                                        aGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa            <  5:348463/149‑1 (MQ=11)
                                                                                                                        aGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa            <  2:289372/149‑1 (MQ=11)
                                                                                                                        aGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa            <  6:346454/149‑1 (MQ=11)
                                                                                                                        aGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCa            <  7:387573/149‑1 (MQ=11)
                                                                                                                          tctcTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGc          <  4:135081/149‑1 (MQ=11)
                                                                                                                          tctcTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGc          <  5:316990/149‑1 (MQ=11)
                                                                                                                            tctGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttctt                                               >  1:226562/1‑110 (MQ=11)
                                                                                                                            tctGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCAt        >  3:170710/1‑149 (MQ=11)
                                                                                                                            tctGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGAGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCAt        >  1:366027/1‑149 (MQ=11)
                                                                                                                             ctGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATc       >  6:370748/1‑149 (MQ=11)
                                                                                                                             ctGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATc       >  7:76821/1‑149 (MQ=11)
                                                                                                                              tGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttcttc                                              <  5:151361/109‑1 (MQ=11)
                                                                                                                                gCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATc       <  3:310797/146‑1 (MQ=11)
                                                                                                                                gCATTCTTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATcc      <  6:286919/147‑1 (MQ=11)
                                                                                                                                  aTTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCttt  >  1:300057/1‑149 (MQ=11)
                                                                                                                                  aTTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCttt  <  8:46511/149‑1 (MQ=11)
                                                                                                                                     |                                                                                                                                                 
TTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTT  >  NC_000913/1635935‑1636213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 2114 total aligned reads displayed.
GAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCAT  >  NC_000913/1635918‑1636219
                                                                                                                                                      |                                                                                                                                                       
   ATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTC                                                                                                                                                      <  NB501016:230:HFVJLAFXY:4:11505:17406:10380/151‑1 (MQ=0)
   ATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTC                                                                                                                                                      <  NB501016:230:HFVJLAFXY:1:21111:14925:9202/151‑1 (MQ=0)
     CAACATTTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGTGGGAGCCCGTCGAGAATAATGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAG                                                                                                                                                    <  NB501016:230:HFVJLAFXY:4:21509:20345:15388/151‑1 (MQ=0)
     CAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTC                                                                                                                                                      >  NB501016:230:HFVJLAFXY:3:11609:21263:13229/1‑149 (MQ=0)
      AACATGTCGGTTTTCCAGTTCAGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTGACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGT                                                                                                                                                   <  NB501016:230:HFVJLAFXY:3:11407:24712:1760/151‑1 (MQ=0)
          TGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCT                                                                                                                                               >  NB501016:230:HFVJLAFXY:3:21409:13589:13852/1‑151 (MQ=0)
             CGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCTATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGT                                                                                                                                             <  NB501016:230:HFVJLAFXY:2:21308:20635:16691/150‑1 (MQ=0)
             CGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTG                                                                                                                                            <  NB501016:230:HFVJLAFXY:4:11402:20432:7508/151‑1 (MQ=0)
             CGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTG                                                                                                                                            <  NB501016:230:HFVJLAFXY:3:11510:8758:5770/151‑1 (MQ=0)
             CGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTG                                                                                                                                            <  NB501016:230:HFVJLAFXY:1:11105:8929:4243/151‑1 (MQ=0)
             CGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTG                                                                                                                                            <  NB501016:230:HFVJLAFXY:4:11411:13779:19811/151‑1 (MQ=0)
             CGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAG                                                                                                                                                    >  NB501016:230:HFVJLAFXY:4:21512:3239:18520/1‑143 (MQ=0)
                  TTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTC                                                                                                                                        <  NB501016:230:HFVJLAFXY:1:21202:6013:11026/150‑1 (MQ=3)
                   TCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGG                                                                                                                                      >  NB501016:230:HFVJLAFXY:2:21207:16820:16376/1‑151 (MQ=7)
                   TCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGG                                                                                                                                      >  NB501016:230:HFVJLAFXY:3:11508:10586:6516/1‑151 (MQ=7)
                   TCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCG                                                                                                                                       <  NB501016:230:HFVJLAFXY:3:21403:2551:11498/150‑1 (MQ=5)
                    CCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGG                                                                                                                                      <  NB501016:230:HFVJLAFXY:2:11303:20402:16357/150‑1 (MQ=7)
                     CAGTTCCGGAAAACGCCGCTGCACCGACGGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCACTGGCATTCTTCAGTTCCTGTGCGTCGGCC                                                                                                                                    >  NB501016:230:HFVJLAFXY:1:21303:21929:20106/1‑151 (MQ=6)
                     CAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCC                                                                                                                                    <  NB501016:230:HFVJLAFXY:4:21408:10399:11332/151‑1 (MQ=7)
                     CAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCC                                                                                                                                    >  NB501016:230:HFVJLAFXY:1:21107:6423:12647/1‑151 (MQ=7)
                      AGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCT                                                                                                                                   >  NB501016:230:HFVJLAFXY:1:11203:12428:16868/1‑151 (MQ=7)
                       GTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACCGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTG                                                                                                                                  >  NB501016:230:HFVJLAFXY:1:21309:20832:13923/1‑151 (MQ=7)
                       GTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTG                                                                                                                                  <  NB501016:230:HFVJLAFXY:4:21412:11002:3609/151‑1 (MQ=7)
                          CCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGC                                                                                                                               <  NB501016:230:HFVJLAFXY:3:21611:20350:5561/151‑1 (MQ=7)
                          CCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCG                                                                                                                                <  NB501016:230:HFVJLAFXY:4:21503:23236:1965/150‑1 (MQ=7)
                          CCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCG                                                                                                                                <  NB501016:230:HFVJLAFXY:3:11501:5470:5758/150‑1 (MQ=7)
                           CGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGTGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGC                                                                                                                               >  NB501016:230:HFVJLAFXY:1:11107:18558:12266/1‑150 (MQ=7)
                           CGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGC                                                                                                                               >  NB501016:230:HFVJLAFXY:3:11605:11591:17247/1‑150 (MQ=7)
                           CGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGC                                                                                                                               >  NB501016:230:HFVJLAFXY:1:11302:16556:16339/1‑150 (MQ=7)
                           CGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGG                                                                                                                                      <  NB501016:230:HFVJLAFXY:2:21101:4537:9522/143‑1 (MQ=7)
                             GAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCTGGGTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACG                                                                                                                            <  NB501016:230:HFVJLAFXY:3:11607:3449:11079/151‑1 (MQ=6)
                             GAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACG                                                                                                                            >  NB501016:230:HFVJLAFXY:1:21104:21283:7441/1‑151 (MQ=7)
                              AAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGcaggccgacgcacag                                                                                                                           <  NB501016:230:HFVJLAFXY:3:21606:22128:15044/151‑16 (MQ=0)
                               AAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGTAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCG                                                                                                                          <  NB501016:230:HFVJLAFXY:1:11108:10589:12373/151‑1 (MQ=7)
                                 ACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTA                                                                                                                        <  NB501016:230:HFVJLAFXY:4:21508:13310:15722/151‑1 (MQ=7)
                                  CGCCGCTGCACCGACAGGGGGAGCCCGTCGAGACTACTGGCAATTTCACCTGCGATCCGCGACCGCACGAAAGTACAGAATGCGGGTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGcaggccgacgcacaggaac                                                                                                                       <  NB501016:230:HFVJLAFXY:4:21501:1285:13176/151‑20 (MQ=0)
                                  CGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTACTGTGCGTCGGCCTGCGCACGCGTA                                                                                                                        >  NB501016:230:HFVJLAFXY:2:11103:4990:5820/1‑150 (MQ=7)
                                   GCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGcaggccgacgcacaggaact                                                                                                                      <  NB501016:230:HFVJLAFXY:2:21304:17818:15388/151‑21 (MQ=0)
                                   GCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAG                                                                                                                      <  NB501016:230:HFVJLAFXY:3:21511:18515:15336/151‑1 (MQ=7)
                                   GCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAG                                                                                                                      <  NB501016:230:HFVJLAFXY:4:11606:20187:1795/151‑1 (MQ=7)
                                    CCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGT                                                                                                                     <  NB501016:230:HFVJLAFXY:3:11606:20831:14679/151‑1 (MQ=7)
                                    CCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGT                                                                                                                     >  NB501016:230:HFVJLAFXY:2:11307:13512:11845/1‑151 (MQ=7)
                                    CCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGT                                                                                                                     <  NB501016:230:HFVJLAFXY:2:21108:17906:18045/151‑1 (MQ=7)
                                     CGCTGCACCGACAGGGGGAGCCCGTCGAGGATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTC                                                                                                                    <  NB501016:230:HFVJLAFXY:2:21112:5950:17139/151‑1 (MQ=7)
                                     CGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCGTGCGCACGCGTAAGTC                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11105:17859:2151/151‑1 (MQ=7)
                                     CGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTC                                                                                                                    >  NB501016:230:HFVJLAFXY:2:11304:5577:5951/1‑151 (MQ=7)
                                     CGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTC                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11310:18761:14215/151‑1 (MQ=7)
                                     CGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTC                                                                                                                    <  NB501016:230:HFVJLAFXY:1:21209:10424:12503/151‑1 (MQ=7)
                                      GCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCG                                                                                                                   <  NB501016:230:HFVJLAFXY:3:21510:16927:14965/151‑1 (MQ=7)
                                         GCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATG                                                                                                                <  NB501016:230:HFVJLAFXY:1:11211:15572:14891/151‑1 (MQ=7)
                                         GCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATG                                                                                                                <  NB501016:230:HFVJLAFXY:1:21103:20334:18507/151‑1 (MQ=7)
                                         GCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGAT                                                                                                                 <  NB501016:230:HFVJLAFXY:4:21505:14642:19781/150‑1 (MQ=7)
                                          CACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCTGCGACAGCACGAAAGTACAGCATGCGGTTTCCTCCACTTGATCTGAGTCTCTGGTAGTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGG                                                                                                               <  NB501016:230:HFVJLAFXY:3:21602:22354:3046/151‑1 (MQ=5)
                                          CACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGG                                                                                                               >  NB501016:230:HFVJLAFXY:2:11102:18667:13922/1‑151 (MQ=7)
                                          CACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGG                                                                                                               <  NB501016:230:HFVJLAFXY:3:11612:21272:9442/151‑1 (MQ=7)
                                          CACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATG                                                                                                                <  NB501016:230:HFVJLAFXY:3:11406:14835:1041/150‑1 (MQ=7)
                                          CACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTACACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGG                                                                                                               <  NB501016:230:HFVJLAFXY:1:21307:5616:16493/151‑1 (MQ=7)
                                          CACCGACAGGGGGAGCCCGTCGAGAATACGGGCAATTTCACCTGCGATCCGCGAGAGGACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCTTTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGG                                                                                                               <  NB501016:230:HFVJLAFXY:4:21601:24380:15249/151‑1 (MQ=6)
                                           ACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGC                                                                                                              <  NB501016:230:HFVJLAFXY:1:11109:13698:4428/151‑1 (MQ=7)
                                            CCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCG                                                                                                             <  NB501016:230:HFVJLAFXY:4:21607:19788:14761/151‑1 (MQ=7)
                                            CCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCG                                                                                                             <  NB501016:230:HFVJLAFXY:1:11212:5751:12550/151‑1 (MQ=7)
                                            CCGACAGGGGAAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCG                                                                                                             <  NB501016:230:HFVJLAFXY:4:11410:12288:14997/151‑1 (MQ=7)
                                                   GGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTAC                                                                                                      <  NB501016:230:HFVJLAFXY:2:21208:15244:2387/151‑1 (MQ=7)
                                                   GGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTAC                                                                                                      <  NB501016:230:HFVJLAFXY:1:21306:3982:18928/151‑1 (MQ=7)
                                                    GGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCAACACTTCAGCGGAGTCTCTGGCATTCTTCAGTTGCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACT                                                                                                     <  NB501016:230:HFVJLAFXY:2:21106:26795:16934/151‑1 (MQ=6)
                                                    GGGAGCCCGGCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACT                                                                                                     <  NB501016:230:HFVJLAFXY:3:21412:25792:7008/151‑1 (MQ=7)
                                                     GGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTC                                                                                                    <  NB501016:230:HFVJLAFXY:1:11103:1161:16020/151‑1 (MQ=7)
                                                       AGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCCGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCA                                                                                                   >  NB501016:230:HFVJLAFXY:1:21110:16070:8943/1‑150 (MQ=7)
                                                        GCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAT                                                                                                 >  NB501016:230:HFVJLAFXY:2:11309:18713:5273/1‑151 (MQ=7)
                                                         CCCGTCGAGAATACTGGCAATTTAACCTGCGATCCGCGACAGCACGAAAGTACCGAATGCGGTTTCCACCACTTCAGCGGCGTCTATGGCATTCTTAAGTTCCTGTGCGTCGGCCGGCGCACGCGTAAGTAGCTGGCGTTCGTACTgaatt                                                                                                >  NB501016:230:HFVJLAFXY:4:11512:20051:15998/1‑146 (MQ=5)
                                                          CCGTCGAGAATACTTGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAG                                                                                               <  NB501016:230:HFVJLAFXY:4:21603:17920:3380/151‑1 (MQ=7)
                                                          CCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAG                                                                                               <  NB501016:230:HFVJLAFXY:3:11504:15892:6036/151‑1 (MQ=7)
                                                          CCGGCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGATCGTACTCAATAG                                                                                               <  NB501016:230:HFVJLAFXY:3:21612:22291:15998/151‑1 (MQ=7)
                                                           CGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGT                                                                                              >  NB501016:230:HFVJLAFXY:2:21309:4334:13911/1‑151 (MQ=7)
                                                              CGAGAATACTGGCAATTTCACCTGCGCTCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGTCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAtttcc                                                                                           >  NB501016:230:HFVJLAFXY:3:11610:10885:7679/1‑146 (MQ=7)
                                                              CGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCG                                                                                                             <  NB501016:230:HFVJLAFXY:3:21411:15789:2461/133‑1 (MQ=8)
                                                               GAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCT                                                                                          >  NB501016:230:HFVJLAFXY:2:21210:11591:13199/1‑151 (MQ=7)
                                                                AGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTG                                                                                         <  NB501016:230:HFVJLAFXY:2:21311:17448:6457/151‑1 (MQ=7)
                                                                 GAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCTGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGG                                                                                        <  NB501016:230:HFVJLAFXY:4:11510:4093:17830/151‑1 (MQ=7)
                                                                 GAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGG                                                                                        <  NB501016:230:HFVJLAFXY:1:21108:8277:13509/151‑1 (MQ=7)
                                                                    TACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTG                                                                                     >  NB501016:230:HFVJLAFXY:4:11608:10104:13065/1‑151 (MQ=7)
                                                                     ACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGTGTTCGTACTCAATAGTTCCTGGCTGG                                                                                    <  NB501016:230:HFVJLAFXY:3:11412:26481:8877/151‑1 (MQ=7)
                                                                     ACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                    <  NB501016:230:HFVJLAFXY:2:21206:19888:12483/151‑1 (MQ=7)
                                                                      CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                    >  NB501016:230:HFVJLAFXY:2:11211:11818:3600/1‑150 (MQ=7)
                                                                      CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTAGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                    >  NB501016:230:HFVJLAFXY:2:11206:8701:2237/1‑150 (MQ=7)
                                                                      CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTG                                                                                                                                              <  NB501016:230:HFVJLAFXY:3:21401:7228:9870/92‑1 (MQ=0)
                                                                      CTGGCAATTTCACCTGCGAGCCGCGACAGCACGAAAGTACAGAATGCGGGTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAT                                                                                                 <  NB501016:230:HFVJLAFXY:4:11506:26653:18539/137‑1 (MQ=7)
                                                                        GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGA                                                                                 <  NB501016:230:HFVJLAFXY:1:11210:20942:5617/151‑1 (MQ=7)
                                                                        GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAG                                                                                  <  NB501016:230:HFVJLAFXY:3:21406:5688:17872/150‑1 (MQ=7)
                                                                        GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCATGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                    >  NB501016:230:HFVJLAFXY:2:21306:1920:8510/1‑148 (MQ=7)
                                                                         GCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGAT                                                                                <  NB501016:230:HFVJLAFXY:4:21606:18644:19145/151‑1 (MQ=7)
                                                                          CAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATC                                                                               <  NB501016:230:HFVJLAFXY:3:21607:4678:5294/151‑1 (MQ=7)
                                                                          CAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTC                                                                                                                    >  NB501016:230:HFVJLAFXY:4:11502:24474:5593/1‑114 (MQ=8)
                                                                           AATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                    >  NB501016:230:HFVJLAFXY:1:11206:12680:8655/1‑145 (MQ=7)
                                                                            ATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTTCTCAATAGTTCCTGGCTGGAGATCTG                                                                             >  NB501016:230:HFVJLAFXY:4:11609:7576:5156/1‑151 (MQ=7)
                                                                            ATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                             >  NB501016:230:HFVJLAFXY:3:11601:23298:4878/1‑151 (MQ=7)
                                                                            ATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTGCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                             <  NB501016:230:HFVJLAFXY:2:11203:2096:3760/151‑1 (MQ=7)
                                                                            ATTTCACCTGCGATCCGCGACAGAACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                             <  NB501016:230:HFVJLAFXY:4:21410:26817:1393/151‑1 (MQ=7)
                                                                              TTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCGCCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTGCGTACTCAATAGTTCCTGGCTGGAGATCTGC                                                                            <  NB501016:230:HFVJLAFXY:4:21511:14449:9969/150‑1 (MQ=6)
                                                                                CACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTC                                                                         >  NB501016:230:HFVJLAFXY:3:21405:12232:3881/1‑151 (MQ=7)
                                                                                CACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTC                                                                         <  NB501016:230:HFVJLAFXY:4:11602:11519:12480/151‑1 (MQ=7)
                                                                                 ACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCG                                                                        <  NB501016:230:HFVJLAFXY:2:21303:19191:7194/151‑1 (MQ=7)
                                                                                  CCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGC                                                                       >  NB501016:230:HFVJLAFXY:4:11405:3436:12906/1‑151 (MQ=7)
                                                                                  CCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                             <  NB501016:230:HFVJLAFXY:4:11407:8670:1035/145‑1 (MQ=7)
                                                                                   CTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCT                                                                      >  NB501016:230:HFVJLAFXY:3:21604:9294:2997/1‑151 (MQ=7)
                                                                                   CTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCT                                                                      >  NB501016:230:HFVJLAFXY:4:11412:15434:3344/1‑151 (MQ=7)
                                                                                   CTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTC                                                                         >  NB501016:230:HFVJLAFXY:1:11207:4627:20014/1‑148 (MQ=7)
                                                                                     GCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGG                                                                    <  NB501016:230:HFVJLAFXY:3:21508:5361:11121/151‑1 (MQ=7)
                                                                                     GCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGG                                                                    <  NB501016:230:HFVJLAFXY:4:21611:15321:15078/151‑1 (MQ=7)
                                                                                     GCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTG                                                                     <  NB501016:230:HFVJLAFXY:2:21109:9841:16345/150‑1 (MQ=7)
                                                                                     GCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGGCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGG                                                                    <  NB501016:230:HFVJLAFXY:3:11506:14041:9860/151‑1 (MQ=6)
                                                                                      CGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGC                                                                   >  NB501016:230:HFVJLAFXY:1:21205:17580:10345/1‑151 (MQ=7)
                                                                                        ATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCT                                                                 >  NB501016:230:HFVJLAFXY:4:11602:9680:8932/1‑151 (MQ=7)
                                                                                        ATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCG                                                                                                             <  NB501016:230:HFVJLAFXY:4:21605:16154:19812/107‑1 (MQ=9)
                                                                                          CCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGC                                                               <  NB501016:230:HFVJLAFXY:3:11610:4921:19928/151‑1 (MQ=7)
                                                                                            GCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCG                                                             <  NB501016:230:HFVJLAFXY:4:21506:2621:13282/151‑1 (MQ=11)
                                                                                            GCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCG                                                             <  NB501016:230:HFVJLAFXY:1:21102:12573:14490/151‑1 (MQ=11)
                                                                                            GCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGC                                                                   <  NB501016:230:HFVJLAFXY:2:21307:17041:18487/145‑1 (MQ=7)
                                                                                                  GCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCCCGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTC                                                       >  NB501016:230:HFVJLAFXY:4:11409:12034:5081/1‑151 (MQ=19)
                                                                                                  GCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTC                                                       <  NB501016:230:HFVJLAFXY:3:11509:4392:10180/151‑1 (MQ=19)
                                                                                                   CACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGT                                                         <  NB501016:230:HFVJLAFXY:4:21608:1582:14161/148‑1 (MQ=19)
                                                                                                   CACGAAAGTACAGAATGCGGTTTCCACCACTTCAGAGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCT                                                      >  NB501016:230:HFVJLAFXY:1:21301:11095:4892/1‑151 (MQ=19)
                                                                                                    ACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCTATAGTTCCTGGCTGGAGATCTGCCTCGATGGCCGGCCGCAGTTAT                                                      >  NB501016:230:HFVJLAFXY:1:11208:26658:2084/1‑150 (MQ=18)
                                                                                                     CGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCCCGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCCGTTCTTC                                                    >  NB501016:230:HFVJLAFXY:3:11409:11696:18839/1‑151 (MQ=18)
                                                                                                     CGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATTTGCCTCGCTGGCCTGCCGCAGTTCTTC                                                    >  NB501016:230:HFVJLAFXY:3:11402:19782:13615/1‑151 (MQ=19)
                                                                                                     CGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTC                                                    <  NB501016:230:HFVJLAFXY:2:21312:2894:9405/151‑1 (MQ=19)
                                                                                                     CGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCG                                                                                                         <  NB501016:230:HFVJLAFXY:1:11204:4048:15513/98‑1 (MQ=9)
                                                                                                      GAAAGTACTGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCTTTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCA                                                   <  NB501016:230:HFVJLAFXY:1:21204:24643:15972/151‑1 (MQ=18)
                                                                                                      GAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCA                                                   <  NB501016:230:HFVJLAFXY:3:11604:17250:10785/151‑1 (MQ=19)
                                                                                                      GAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCA                                                   <  NB501016:230:HFVJLAFXY:4:11406:23080:17297/151‑1 (MQ=19)
                                                                                                       AAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAA                                                  <  NB501016:230:HFVJLAFXY:4:11403:16176:13573/151‑1 (MQ=19)
                                                                                                          GTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACC                                                >  NB501016:230:HFVJLAFXY:4:11501:22768:15833/1‑150 (MQ=17)
                                                                                                            ACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCC                                             >  NB501016:230:HFVJLAFXY:2:11202:24616:15510/1‑151 (MQ=8)
                                                                                                             CAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCC                                            >  NB501016:230:HFVJLAFXY:2:21104:4312:5373/1‑151 (MQ=6)
                                                                                                             CAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCC                                            >  NB501016:230:HFVJLAFXY:2:21302:24253:3730/1‑151 (MQ=6)
                                                                                                             CAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCT                                                      <  NB501016:230:HFVJLAFXY:1:21210:2408:11069/141‑1 (MQ=20)
                                                                                                               GAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGG                                          >  NB501016:230:HFVJLAFXY:4:21507:19004:8543/1‑151 (MQ=6)
                                                                                                                 ATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCG                                        >  NB501016:230:HFVJLAFXY:1:11104:1993:12132/1‑151 (MQ=6)
                                                                                                                 ATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCG                                        >  NB501016:230:HFVJLAFXY:3:21609:12217:8931/1‑151 (MQ=6)
                                                                                                                  TGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGC                                       >  NB501016:230:HFVJLAFXY:3:11602:5712:1312/1‑151 (MQ=6)
                                                                                                                    CGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAG                                     <  NB501016:230:HFVJLAFXY:1:11304:16529:12868/151‑1 (MQ=6)
                                                                                                                    CGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGG                                          >  NB501016:230:HFVJLAFXY:4:21403:15409:11456/1‑146 (MQ=6)
                                                                                                                    CGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAAC                                                 >  NB501016:230:HFVJLAFXY:4:11503:8151:6400/1‑139 (MQ=20)
                                                                                                                    CGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCT                                                      <  NB501016:230:HFVJLAFXY:4:21405:17222:14519/134‑1 (MQ=20)
                                                                                                                     GGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGC                                    >  NB501016:230:HFVJLAFXY:3:21407:8345:16840/1‑151 (MQ=6)
                                                                                                                      GTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCT                                   >  NB501016:230:HFVJLAFXY:1:21207:3342:11409/1‑151 (MQ=6)
                                                                                                                         TCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCC                                                              >  NB501016:230:HFVJLAFXY:4:11509:16174:4090/1‑121 (MQ=9)
                                                                                                                          CCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTC                               >  NB501016:230:HFVJLAFXY:4:11611:17250:19359/1‑151 (MQ=6)
                                                                                                                          CCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTC                               >  NB501016:230:HFVJLAFXY:3:11511:7294:5954/1‑151 (MQ=6)
                                                                                                                          CCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTT                                <  NB501016:230:HFVJLAFXY:1:11102:11982:2159/150‑1 (MQ=6)
                                                                                                                           CACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                              >  NB501016:230:HFVJLAFXY:2:11106:7712:19056/1‑151 (MQ=6)
                                                                                                                           CACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                              <  NB501016:230:HFVJLAFXY:2:11305:8231:4020/151‑1 (MQ=6)
                                                                                                                           CACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                              <  NB501016:230:HFVJLAFXY:3:21503:15572:2895/151‑1 (MQ=6)
                                                                                                                           CACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                              <  NB501016:230:HFVJLAFXY:2:11205:15480:3335/151‑1 (MQ=6)
                                                                                                                            CCCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGT                             <  NB501016:230:HFVJLAFXY:2:21103:14544:12414/151‑1 (MQ=6)
                                                                                                                            ACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGT                             >  NB501016:230:HFVJLAFXY:4:21409:22632:13246/1‑151 (MQ=6)
                                                                                                                             CCACTTCTGGGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGGCGGGCTGCGCACGCGTAAGTCGATTGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTT                            <  NB501016:230:HFVJLAFXY:3:21506:14362:4622/151‑1 (MQ=5)
                                                                                                                             CCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCTCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTT                            <  NB501016:230:HFVJLAFXY:1:21105:1785:4254/151‑1 (MQ=6)
                                                                                                                             CCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGGGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTT                            <  NB501016:230:HFVJLAFXY:2:11108:5362:7435/151‑1 (MQ=6)
                                                                                                                               ACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCT                          <  NB501016:230:HFVJLAFXY:3:21504:6555:9875/151‑1 (MQ=6)
                                                                                                                               ACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCT                          <  NB501016:230:HFVJLAFXY:1:11307:2819:16180/151‑1 (MQ=6)
                                                                                                                               ACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCT                          <  NB501016:230:HFVJLAFXY:4:21609:7266:3883/151‑1 (MQ=6)
                                                                                                                                CTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTC                         >  NB501016:230:HFVJLAFXY:4:21402:12296:5571/1‑151 (MQ=6)
                                                                                                                                CTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTC                           <  NB501016:230:HFVJLAFXY:3:11404:23292:13586/149‑1 (MQ=6)
                                                                                                                                  TCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTC                         <  NB501016:230:HFVJLAFXY:1:11202:12714:7477/149‑1 (MQ=6)
                                                                                                                                    AGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATT                     >  NB501016:230:HFVJLAFXY:2:21212:17459:12766/1‑151 (MQ=6)
                                                                                                                                    AGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATT                     <  NB501016:230:HFVJLAFXY:2:11301:13331:16061/151‑1 (MQ=6)
                                                                                                                                      CGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTC                   <  NB501016:230:HFVJLAFXY:1:21106:14090:4852/151‑1 (MQ=6)
                                                                                                                                      CGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTG                                                                <  NB501016:230:HFVJLAFXY:2:11208:3920:3367/106‑1 (MQ=8)
                                                                                                                                      CGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGGTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCTACTTTTCGTTCTCAATTTC                   <  NB501016:230:HFVJLAFXY:1:21310:24270:18605/151‑1 (MQ=6)
                                                                                                                                      CGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGCAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTC                   <  NB501016:230:HFVJLAFXY:3:21507:4283:15598/151‑1 (MQ=6)
                                                                                                                                      CGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGGCGGCCGGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTTGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTC                   <  NB501016:230:HFVJLAFXY:3:11411:21476:1560/151‑1 (MQ=6)
                                                                                                                                       GGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCA                  <  NB501016:230:HFVJLAFXY:4:11404:20728:9648/151‑1 (MQ=6)
                                                                                                                                       GGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCA                  <  NB501016:230:HFVJLAFXY:1:11305:5696:3886/151‑1 (MQ=6)
                                                                                                                                        GAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                 <  NB501016:230:HFVJLAFXY:2:11201:14106:18845/151‑1 (MQ=6)
                                                                                                                                        GAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                 <  NB501016:230:HFVJLAFXY:3:11502:6011:10895/151‑1 (MQ=6)
                                                                                                                                        GAGTCGCGGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCGGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                 <  NB501016:230:HFVJLAFXY:1:11311:25270:16850/151‑1 (MQ=6)
                                                                                                                                        GAGGCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGAGGGCGGTCGTCCTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                 <  NB501016:230:HFVJLAFXY:1:11112:25364:19313/151‑1 (MQ=5)
                                                                                                                                         AGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCCCGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGACCTGCCGCAGTTCTTCAACCGCCCGGCGCAGCTATTCGTGCTCAATTTCAGC                >  NB501016:230:HFVJLAFXY:3:11401:18546:11276/1‑151 (MQ=5)
                                                                                                                                         AGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGC                >  NB501016:230:HFVJLAFXY:1:11309:6917:13743/1‑151 (MQ=6)
                                                                                                                                            CTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC             >  NB501016:230:HFVJLAFXY:2:21202:2018:7547/1‑151 (MQ=6)
                                                                                                                                            CTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC             >  NB501016:230:HFVJLAFXY:2:21305:14336:8912/1‑151 (MQ=6)
                                                                                                                                               AGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCATGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCACCCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCT          >  NB501016:230:HFVJLAFXY:2:21211:12392:10101/1‑151 (MQ=6)
                                                                                                                                                GGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTT         >  NB501016:230:HFVJLAFXY:1:21312:24636:5688/1‑151 (MQ=6)
                                                                                                                                                GGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTT         >  NB501016:230:HFVJLAFXY:1:21112:20932:1764/1‑151 (MQ=6)
                                                                                                                                                GGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC           <  NB501016:230:HFVJLAFXY:2:21204:10436:15888/149‑1 (MQ=6)
                                                                                                                                                GGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTGCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGAATCCCTT         >  NB501016:230:HFVJLAFXY:2:11111:25884:19329/1‑151 (MQ=6)
                                                                                                                                                  CATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACctccagatcggaagagcacacgtcctgaactccagtcaccgg       >  NB501016:230:HFVJLAFXY:1:21203:24965:8661/1‑109 (MQ=10)
                                                                                                                                                  CATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTC       >  NB501016:230:HFVJLAFXY:4:11604:8184:18800/1‑151 (MQ=6)
                                                                                                                                                  CATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTC       >  NB501016:230:HFVJLAFXY:4:11507:3856:14598/1‑151 (MQ=6)
                                                                                                                                                  CATTCTTCAGTTCCTGTGCGTAGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTC       <  NB501016:230:HFVJLAFXY:3:21601:24746:6126/151‑1 (MQ=6)
                                                                                                                                                  CATTCTTAAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAAAGTTACTGGCTGGAGATATGCCTCCCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTATCGTTCTCAATTTCAGCATCCCTTTC       >  NB501016:230:HFVJLAFXY:4:21407:20345:9891/1‑151 (MQ=5)
                                                                                                                                                   ATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG      <  NB501016:230:HFVJLAFXY:2:11110:20881:5639/151‑1 (MQ=6)
                                                                                                                                                   ATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG      >  NB501016:230:HFVJLAFXY:2:11312:1756:18849/1‑151 (MQ=6)
                                                                                                                                                   ATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATATGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG      >  NB501016:230:HFVJLAFXY:2:21205:2513:16942/1‑151 (MQ=6)
                                                                                                                                                   ATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGGAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG      <  NB501016:230:HFVJLAFXY:1:21304:24743:4338/151‑1 (MQ=6)
                                                                                                                                                     TCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC    >  NB501016:230:HFVJLAFXY:1:11101:10299:7719/1‑151 (MQ=7)
                                                                                                                                                      CTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC    >  NB501016:230:HFVJLAFXY:4:21512:1404:10731/1‑150 (MQ=7)
                                                                                                                                                      CTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC    >  NB501016:230:HFVJLAFXY:3:21610:14426:18465/1‑150 (MQ=7)
                                                                                                                                                      CTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTACG                              >  NB501016:230:HFVJLAFXY:1:11110:3707:1220/1‑124 (MQ=7)
                                                                                                                                                      |                                                                                                                                                       
GAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCAT  >  NC_000913/1635918‑1636219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑