Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,636,074 C→T 100% pseudogene (294/528 nt) nohQ ← pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,636,0740CT99.0% 7667.9 / 19.0 3777pseudogene (294/528 nt)nohQpseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base T (1820/1897);  total (1849/1928)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 3777 total aligned reads displayed.

TTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATAC  >  NC_000913/1635935‑1636221
                                                                                                                                           |                                                                                                                                                   
  tCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGt                                                                                                                                           <  4:34750/148‑1 (MQ=9)
  tCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGt                                                                                                                                           <  1:333080/148‑1 (MQ=9)
   ccAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCg                                                                                                                                         >  8:127519/1‑149 (MQ=12)
   ccAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCg                                                                                                                                         >  6:44335/1‑149 (MQ=12)
     aGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGc                                                                                                                                       >  7:13370/1‑149 (MQ=12)
      gTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGcc                                                                                                                                      >  7:188173/1‑149 (MQ=12)
       ttCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCt                                                                                                                                     >  1:196102/1‑149 (MQ=12)
       ttCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCt                                                                                                                                     >  5:81410/1‑149 (MQ=12)
          cGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTgcg                                                                                                                                  >  8:367905/1‑149 (MQ=12)
             aaaaCGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCAc                                                                                                                               >  7:159257/1‑149 (MQ=12)
               aaCGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCAcgc                                                                                                                             <  6:137007/149‑1 (MQ=12)
                   ccgcTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTaa                                                                                                                         <  4:138248/149‑1 (MQ=12)
                     gcTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGt                                                                                                                       >  6:344127/1‑149 (MQ=12)
                     gcTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGt                                                                                                                       <  7:331285/149‑1 (MQ=12)
                        gCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTaa                                                                                                                         >  6:110695/1‑144 (MQ=12)
                         cACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGa                                                                                                                    <  5:20835/148‑1 (MQ=12)
                         cACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGAt                                                                                                                   <  5:260256/149‑1 (MQ=12)
                         cACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGAt                                                                                                                   <  6:7821/149‑1 (MQ=12)
                         cACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGAt                                                                                                                   <  8:253064/149‑1 (MQ=12)
                          aCCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATg                                                                                                                  <  7:74169/149‑1 (MQ=12)
                            cGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGc                                                                                                                <  4:73859/149‑1 (MQ=12)
                              aCAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGt                                                                                                              >  6:316990/1‑149 (MQ=12)
                                aGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTc                                                                                                            <  3:265847/149‑1 (MQ=12)
                                 gggggAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCg                                                                                                           >  5:102770/1‑149 (MQ=12)
                                        cccGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTaa                                                                                                                         >  1:126952/1‑128 (MQ=17)
                                        cccGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCaa                                                                                                    >  4:282487/1‑149 (MQ=17)
                                          cGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATa                                                                                                  <  6:161299/149‑1 (MQ=17)
                                          cGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTGCGTACGCaa                                                                                                    >  6:183880/1‑147 (MQ=255)
                                              gagaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGttc                                                                                              >  2:176727/1‑147 (MQ=12)
                                               agaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTtcc                                                                                             <  3:143165/149‑1 (MQ=12)
                                                gaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAATTCGATGGCGTTCGTACTCAATAGTTCct                                                                                            <  5:350828/149‑1 (MQ=11)
                                                gaATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACAACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCct                                                                                            >  2:116009/1‑149 (MQ=255)
                                                 aaTACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctg                                                                                           <  1:245491/149‑1 (MQ=12)
                                                     cTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggct                                                                                        <  3:108391/148‑1 (MQ=12)
                                                     cTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggct                                                                                        <  1:90895/148‑1 (MQ=12)
                                                      tGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctg                                                                                       >  8:70992/1‑148 (MQ=12)
                                                      tGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctg                                                                                       >  1:66145/1‑148 (MQ=12)
                                                        gCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCctggctg                                                                                       >  5:286919/1‑146 (MQ=12)
                                                        gCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGga                                                                                     <  4:255905/148‑1 (MQ=12)
                                                         cAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGgaga                                                                                   <  8:34082/149‑1 (MQ=255)
                                                          aaTTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTAc                                                                                                        <  3:332904/127‑1 (MQ=17)
                                                           aTTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATc                                                                                 >  8:1012/1‑149 (MQ=12)
                                                            tttCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCt                                                                                <  4:194647/149‑1 (MQ=12)
                                                            tttCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCt                                                                                <  2:147045/149‑1 (MQ=12)
                                                            tttCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCt                                                                                >  5:126868/1‑149 (MQ=12)
                                                            tttCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGGTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCt                                                                                <  6:213037/149‑1 (MQ=255)
                                                               cACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTgc                                                                              <  6:232739/148‑2 (MQ=12)
                                                               cACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTgc                                                                              <  3:282932/148‑2 (MQ=12)
                                                                     cGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCt                                                                        <  3:230681/148‑1 (MQ=255)
                                                                           gcgACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGCCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGc                                                                 >  4:304537/1‑149 (MQ=11)
                                                                                  cACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGtt                                                          >  2:379278/1‑149 (MQ=34)
                                                                                  cACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTc                                                                                                            <  2:347962/99‑1 (MQ=16)
                                                                                  cACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGcc                                                                <  8:164678/143‑1 (MQ=255)
                                                                                    cGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttct                                                        <  3:353922/149‑1 (MQ=255)
                                                                                       aaGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCa                                                     <  4:166261/149‑1 (MQ=255)
                                                                                        aGTACAGAATGCGGTTGCCACCCCTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTGCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCaa                                                    <  5:373104/149‑1 (MQ=11)
                                                                                           aCAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAAcct                                                 >  5:54305/1‑147 (MQ=255)
                                                                                             aGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTcc                                               >  3:311106/1‑149 (MQ=11)
                                                                                             aGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTcc                                               >  2:288894/1‑149 (MQ=11)
                                                                                             aGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTcc                                               >  8:43656/1‑149 (MQ=11)
                                                                                               aaTGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATg                                                                                                                  >  2:78004/1‑80 (MQ=255)
                                                                                               aaTGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCg                                                                          >  5:1522/1‑120 (MQ=11)
                                                                                               aaTGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttcttc                                                      <  3:98951/140‑1 (MQ=255)
                                                                                                aTGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGttcttc                                                      >  6:26034/1‑139 (MQ=255)
                                                                                                  gCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGgcg                                          >  6:366035/1‑149 (MQ=11)
                                                                                                    ggTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCg                                             >  7:219246/1‑144 (MQ=11)
                                                                                                     gTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAg                                       >  8:287692/1‑149 (MQ=11)
                                                                                                          caccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCttt                                   <  7:307893/148‑1 (MQ=11)
                                                                                                           accacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATGTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGgc                                           <  4:44915/139‑1 (MQ=11)
                                                                                                           accacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTc                                 >  4:323186/1‑149 (MQ=11)
                                                                                                           accacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTc                                 >  7:102881/1‑149 (MQ=11)
                                                                                                           accacTTCAGCGGAATCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAt                                                                                                   >  7:382151/1‑83 (MQ=11)
                                                                                                            ccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCg                                <  1:100552/149‑1 (MQ=11)
                                                                                                            ccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCg                                >  7:253902/1‑149 (MQ=11)
                                                                                                            ccacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCg                                <  5:318258/149‑1 (MQ=11)
                                                                                                             cacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCTCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGt                               <  1:225191/149‑1 (MQ=11)
                                                                                                             cacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGt                               >  2:231317/1‑149 (MQ=11)
                                                                                                             cacTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGAGGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTATTCAACCTCCCGGCGCAGCTTTTCgg                               >  2:258800/1‑148 (MQ=11)
                                                                                                              acTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGtt                              <  7:359991/149‑1 (MQ=11)
                                                                                                                ttCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTc                                                                                                            <  1:162321/69‑1 (MQ=255)
                                                                                                                  cAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTtct                            <  2:324006/147‑1 (MQ=11)
                                                                                                                    gCGGAGTCTCTGGCATTCTTCAGTTCCTTTGCGTCGGCCTGCGCACGCGTAAGTCGATAGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAAt                        <  2:44966/149‑1 (MQ=11)
                                                                                                                    gCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAAt                        <  8:314132/149‑1 (MQ=11)
                                                                                                                     cGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAAtt                       >  3:45229/1‑149 (MQ=11)
                                                                                                                     cGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAAtt                       <  4:229612/149‑1 (MQ=11)
                                                                                                                       gAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTc                     <  1:277003/149‑1 (MQ=11)
                                                                                                                        aGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGAGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCa                    <  2:207925/149‑1 (MQ=37)
                                                                                                                          tctcTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGc                  <  7:48303/149‑1 (MQ=11)
                                                                                                                            tctGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCAt                >  3:170710/1‑149 (MQ=11)
                                                                                                                              tGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCa                 <  5:180305/146‑1 (MQ=11)
                                                                                                                              tGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATcc              >  6:280882/1‑149 (MQ=11)
                                                                                                                              tGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATcc              >  1:298192/1‑149 (MQ=11)
                                                                                                                                gCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCt            >  3:71247/1‑149 (MQ=11)
                                                                                                                                gCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCt            >  1:393423/1‑149 (MQ=11)
                                                                                                                                   ttcttCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTc         <  1:363706/146‑1 (MQ=11)
                                                                                                                                    tcttCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGtt                              <  5:232313/125‑1 (MQ=11)
                                                                                                                                       tCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCa     >  8:229607/1‑149 (MQ=255)
                                                                                                                                        cAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGTCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCgg       <  8:19792/146‑1 (MQ=11)
                                                                                                                                        cAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCgg       <  3:198383/146‑1 (MQ=255)
                                                                                                                                        cAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCgg       <  7:282025/146‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
TTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATAC  >  NC_000913/1635935‑1636221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 2110 total aligned reads displayed.
AACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCAT  >  NC_000913/1635924‑1636224
                                                                                                                                                      |                                                                                                                                                      
    TGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCT                                                                                                                                                    >  NB501016:230:HFVJLAFXY:2:21105:1134:12038/1‑151 (MQ=0)
     GTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGCCAGGGGGAGCCCGTCGAGAATACTGGGAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCCCCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTG                                                                                                                                                   >  NB501016:230:HFVJLAFXY:2:21108:19789:14585/1‑151 (MQ=0)
     GTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGTGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCT                                                                                                                                                    <  NB501016:230:HFVJLAFXY:4:11407:23489:13747/150‑1 (MQ=0)
       CGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTG                                                                                                                                                 <  NB501016:230:HFVJLAFXY:1:21210:16457:13113/151‑1 (MQ=0)
         GTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCG                                                                                                                                               <  NB501016:230:HFVJLAFXY:3:11607:21277:7515/151‑1 (MQ=0)
         GTGTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCAGTCGAGAATACTGTCAAGTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCG                                                                                                                                               <  NB501016:230:HFVJLAFXY:1:11204:6930:13459/151‑1 (MQ=0)
           TTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTC                                                                                                                                             >  NB501016:230:HFVJLAFXY:2:21307:1517:14669/1‑151 (MQ=3)
            TTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTC                                                                                                                                             <  NB501016:230:HFVJLAFXY:1:21202:6013:11026/150‑1 (MQ=3)
             TCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGG                                                                                                                                           >  NB501016:230:HFVJLAFXY:2:21207:16820:16376/1‑151 (MQ=7)
             TCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCG                                                                                                                                            <  NB501016:230:HFVJLAFXY:3:21604:14935:14409/150‑1 (MQ=5)
              CCAGTTCCGGAAAACGCCGCTGCTCCGACAGGTGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTAGAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGC                                                                                                                                          <  NB501016:230:HFVJLAFXY:4:21507:4155:5719/151‑1 (MQ=6)
              CCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCCCCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGC                                                                                                                                          >  NB501016:230:HFVJLAFXY:3:11401:16115:1712/1‑151 (MQ=7)
              CCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGC                                                                                                                                          >  NB501016:230:HFVJLAFXY:1:21307:4333:10238/1‑151 (MQ=7)
              CCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGGTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGG                                                                                                                                           <  NB501016:230:HFVJLAFXY:2:11108:8176:14404/150‑1 (MQ=7)
                   cgagatcatagccggtgactggagttcagacgttgtgctcttccgatctCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCG                                                                                                                                     <  NB501016:230:HFVJLAFXY:1:11206:17880:11889/102‑1 (MQ=9)
                    CCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACCGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:3:21507:8523:6723/1‑151 (MQ=7)
                    CCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGC                                                                                                                                    >  NB501016:230:HFVJLAFXY:1:11301:14141:13476/1‑151 (MQ=7)
                    CCGGAAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGC                                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11307:22120:11583/151‑1 (MQ=7)
                        AAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGC                                                                                                                                >  NB501016:230:HFVJLAFXY:4:21512:18865:4262/1‑151 (MQ=7)
                        AAAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGC                                                                                                                                >  NB501016:230:HFVJLAFXY:4:11411:5280:15685/1‑151 (MQ=7)
                         AAACGCCGCTGCACCGACAGGGGGGGCCCGTCGAGAATACTGGCAATTTCACCTGCGCTCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACAACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCCCGCG                                                                                                                               >  NB501016:230:HFVJLAFXY:4:21607:20916:12333/1‑151 (MQ=6)
                         AAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCG                                                                                                                               <  NB501016:230:HFVJLAFXY:1:21204:2371:17029/151‑1 (MQ=7)
                         AAACGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCG                                                                                                                               <  NB501016:230:HFVJLAFXY:1:11211:3581:10585/151‑1 (MQ=7)
                            CGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCCCCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTA                                                                                                                             >  NB501016:230:HFVJLAFXY:2:11306:16532:13114/1‑150 (MQ=7)
                            CGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCCGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTA                                                                                                                             >  NB501016:230:HFVJLAFXY:1:21109:1656:6157/1‑150 (MQ=7)
                            CGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTA                                                                                                                             >  NB501016:230:HFVJLAFXY:1:21110:9873:9893/1‑150 (MQ=7)
                            CGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTA                                                                                                                             >  NB501016:230:HFVJLAFXY:1:21103:19886:9835/1‑150 (MQ=7)
                            CGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTACTGTGCGTCGGCCTGCGCACGCGTA                                                                                                                             >  NB501016:230:HFVJLAFXY:2:11103:4990:5820/1‑150 (MQ=7)
                            CGCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGATTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGcaggccgacgcacaggaac                                                                                                                            <  NB501016:230:HFVJLAFXY:4:21603:26357:15924/151‑20 (MQ=0)
                             GCCGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAG                                                                                                                           <  NB501016:230:HFVJLAFXY:4:11606:20187:1795/151‑1 (MQ=7)
                             GCCGCTGCACCGACAGGGGGAGCCCGGCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGGTTCCACCACTTCAGCGGAGGCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAG                                                                                                                           <  NB501016:230:HFVJLAFXY:1:11107:2076:6631/151‑1 (MQ=6)
                              CCGCTGCACCGACAGGGGGCGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCATGTGCGTCGGCCTGCGCACGCGTAAGT                                                                                                                          >  NB501016:230:HFVJLAFXY:2:11202:5181:2353/1‑151 (MQ=6)
                               CGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCGTGCGCACGCGTAAGTC                                                                                                                         <  NB501016:230:HFVJLAFXY:2:11105:17859:2151/151‑1 (MQ=7)
                               CGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTC                                                                                                                         <  NB501016:230:HFVJLAFXY:4:11602:11517:5362/151‑1 (MQ=7)
                               CGCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTC                                                                                                                         <  NB501016:230:HFVJLAFXY:2:21112:7592:19699/151‑1 (MQ=7)
                                GCTGCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCG                                                                                                                        <  NB501016:230:HFVJLAFXY:1:21112:15613:18617/151‑1 (MQ=7)
                                   GCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCCTTCTTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATG                                                                                                                     <  NB501016:230:HFVJLAFXY:3:11602:1434:18685/151‑1 (MQ=6)
                                   GCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATG                                                                                                                     <  NB501016:230:HFVJLAFXY:3:21609:23066:16598/151‑1 (MQ=7)
                                   GCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATG                                                                                                                     <  NB501016:230:HFVJLAFXY:3:21502:24780:6894/151‑1 (MQ=7)
                                   GCACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATG                                                                                                                     <  NB501016:230:HFVJLAFXY:1:11302:25763:17833/151‑1 (MQ=7)
                                    CACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGG                                                                                                                    >  NB501016:230:HFVJLAFXY:2:11102:18667:13922/1‑151 (MQ=7)
                                    CACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGG                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11311:1215:16056/151‑1 (MQ=7)
                                    CACCGACAGGGGGAGCCCGTCGAGAATACTGGCAACTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGG                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11502:18466:10325/151‑1 (MQ=7)
                                     ACCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGC                                                                                                                   <  NB501016:230:HFVJLAFXY:1:11109:13698:4428/151‑1 (MQ=7)
                                      CCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCG                                                                                                                  <  NB501016:230:HFVJLAFXY:3:11501:14949:7570/151‑1 (MQ=7)
                                      CCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCG                                                                                                                  >  NB501016:230:HFVJLAFXY:4:21506:5699:15134/1‑151 (MQ=7)
                                      CCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCA                                                                                                                  <  NB501016:230:HFVJLAFXY:3:21510:19488:9971/151‑1 (MQ=7)
                                      CCGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGC                                                                                                                   >  NB501016:230:HFVJLAFXY:4:11404:15497:14868/1‑150 (MQ=7)
                                       CGACAGGGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGT                                                                                                                 <  NB501016:230:HFVJLAFXY:3:11603:20209:11348/151‑1 (MQ=7)
                                             GGGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTAC                                                                                                           <  NB501016:230:HFVJLAFXY:2:11312:4611:9170/151‑1 (MQ=7)
                                              GGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACT                                                                                                          <  NB501016:230:HFVJLAFXY:2:21308:18746:4167/151‑1 (MQ=7)
                                              GGGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAG                                                                                                                           <  NB501016:230:HFVJLAFXY:3:21611:3950:18744/134‑1 (MQ=7)
                                               GGAGCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTC                                                                                                         >  NB501016:230:HFVJLAFXY:3:21509:25708:11734/1‑151 (MQ=7)
                                                  GCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAT                                                                                                      <  NB501016:230:HFVJLAFXY:2:11206:12510:18130/151‑1 (MQ=7)
                                                  GCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAT                                                                                                      <  NB501016:230:HFVJLAFXY:4:11408:7991:12318/151‑1 (MQ=7)
                                                   CCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAT                                                                                                      >  NB501016:230:HFVJLAFXY:1:11104:22458:9718/1‑150 (MQ=7)
                                                   CCCGTCGAGAATACTGGCAATTTAACCTGCGATCCGCGACAGCACGAAAGTACCGAATGCGGTTTCCACCACTTCAGCGGCGTCTATGGCATTCTTAAGTTCCTGTGCGTCGGCCGGCGCACGCGTAAGTAGCTGGCGTTCGTACTgaatt                                                                                                     >  NB501016:230:HFVJLAFXY:4:11512:20051:15998/1‑146 (MQ=5)
                                                    CCGTCGAGAATACTGGGAATTTCAGCTGCGATGCTCGACAGCACGAAAGTACTGTATGCGGTTTCCTGCACTTCAGCGGAGTCTCTGGCATTGTTCAGTTCCTGTGCGTCGACCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAG                                                                                                    <  NB501016:230:HFVJLAFXY:3:11612:15090:17931/151‑1 (MQ=5)
                                                    CCGTCGAGAATACTGGCAATTTTACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAG                                                                                                    <  NB501016:230:HFVJLAFXY:4:11411:10097:19689/151‑1 (MQ=7)
                                                    CCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGTAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAG                                                                                                    <  NB501016:230:HFVJLAFXY:4:21606:24851:2572/151‑1 (MQ=7)
                                                    CCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAG                                                                                                    <  NB501016:230:HFVJLAFXY:3:11402:14832:17249/151‑1 (MQ=8)
                                                    CCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAT                                                                                                      >  NB501016:230:HFVJLAFXY:2:11201:2098:12014/1‑149 (MQ=7)
                                                     CGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGT                                                                                                   >  NB501016:230:HFVJLAFXY:2:21211:12052:4282/1‑151 (MQ=7)
                                                      GTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTT                                                                                                  >  NB501016:230:HFVJLAFXY:4:11604:6900:18053/1‑151 (MQ=7)
                                                        CGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCATGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCC                                                                                                <  NB501016:230:HFVJLAFXY:1:21312:3554:9753/151‑1 (MQ=7)
                                                        CGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCC                                                                                                >  NB501016:230:HFVJLAFXY:4:11405:13233:9627/1‑151 (MQ=7)
                                                        CGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGACTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCC                                                                                                >  NB501016:230:HFVJLAFXY:3:11411:18281:13677/1‑151 (MQ=7)
                                                           GAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGG                                                                                             <  NB501016:230:HFVJLAFXY:4:21412:16039:2694/151‑1 (MQ=7)
                                                            AATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGG                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11110:26470:8275/127‑1 (MQ=8)
                                                             ATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGACTGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCT                                                                                           <  NB501016:230:HFVJLAFXY:1:21304:20909:2705/151‑1 (MQ=7)
                                                             ATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCA                                                                                                        >  NB501016:230:HFVJLAFXY:2:21306:15740:6062/1‑138 (MQ=8)
                                                              TACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTG                                                                                          >  NB501016:230:HFVJLAFXY:4:11608:10104:13065/1‑151 (MQ=7)
                                                               ACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                         <  NB501016:230:HFVJLAFXY:1:11103:14589:13677/151‑1 (MQ=7)
                                                               ACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                         <  NB501016:230:HFVJLAFXY:4:11402:4111:7528/151‑1 (MQ=7)
                                                                CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                         >  NB501016:230:HFVJLAFXY:3:21404:3568:14475/1‑150 (MQ=7)
                                                                CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGT                                                                                                             >  NB501016:230:HFVJLAFXY:1:21108:19892:5039/1‑130 (MQ=8)
                                                                CTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGAGTTCGTTCTCAATAGTtactggatgg                                                                                         >  NB501016:230:HFVJLAFXY:4:11609:26004:7797/1‑140 (MQ=7)
                                                                CTGGCAATTTCACCTGCGAGCCGCGACAGCACGAAAGTACAGAATGCGGGTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAT                                                                                                      <  NB501016:230:HFVJLAFXY:4:11506:26653:18539/137‑1 (MQ=7)
                                                                  GGCAATTTCCCCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGagatcggaagagccacacgtctg                                                                                       >  NB501016:230:HFVJLAFXY:3:11412:18734:1350/1‑127 (MQ=7)
                                                                  GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAG                                                                                       <  NB501016:230:HFVJLAFXY:2:11303:7737:20097/150‑1 (MQ=7)
                                                                  GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGA                                                                                        <  NB501016:230:HFVJLAFXY:4:21602:2564:10521/149‑1 (MQ=7)
                                                                  GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                         >  NB501016:230:HFVJLAFXY:2:21106:8116:14472/1‑148 (MQ=7)
                                                                  GGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTC                                                                                                                         >  NB501016:230:HFVJLAFXY:2:21312:25439:10715/1‑116 (MQ=8)
                                                                   GCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                         >  NB501016:230:HFVJLAFXY:1:21101:22839:11231/1‑147 (MQ=7)
                                                                    CAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATC                                                                                    <  NB501016:230:HFVJLAFXY:1:21105:16154:1928/151‑1 (MQ=7)
                                                                         TCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCC                                                                                <  NB501016:230:HFVJLAFXY:2:21208:16517:10912/150‑1 (MQ=7)
                                                                         TCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCC                                                                                <  NB501016:230:HFVJLAFXY:1:11108:11007:16388/150‑1 (MQ=7)
                                                                          CACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCCCCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAAGAGTTCCTGGCTGGAGATCTGCCTC                                                                              >  NB501016:230:HFVJLAFXY:3:21610:25698:5689/1‑151 (MQ=6)
                                                                          CACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTC                                                                              <  NB501016:230:HFVJLAFXY:3:21408:21252:12768/151‑1 (MQ=7)
                                                                          CACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGG                                                                                         >  NB501016:230:HFVJLAFXY:3:21406:10051:13278/1‑140 (MQ=7)
                                                                           ACCTGCGAGCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCG                                                                             <  NB501016:230:HFVJLAFXY:3:21403:14121:13554/151‑1 (MQ=6)
                                                                              TGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTG                                                                          >  NB501016:230:HFVJLAFXY:4:11406:18801:10152/1‑151 (MQ=7)
                                                                               GCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGG                                                                         <  NB501016:230:HFVJLAFXY:2:21310:4471:14408/151‑1 (MQ=7)
                                                                               GCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGG                                                                         <  NB501016:230:HFVJLAFXY:3:21607:13309:10401/151‑1 (MQ=7)
                                                                               GCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTG                                                                          <  NB501016:230:HFVJLAFXY:3:11503:6784:5429/150‑1 (MQ=7)
                                                                                CGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGC                                                                        >  NB501016:230:HFVJLAFXY:4:21409:23027:2805/1‑151 (MQ=7)
                                                                                CGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCT                                                                           >  NB501016:230:HFVJLAFXY:1:11207:4813:5490/1‑148 (MQ=7)
                                                                                 GATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCC                                                                       <  NB501016:230:HFVJLAFXY:1:21306:16275:13619/151‑1 (MQ=7)
                                                                                  ATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCT                                                                      >  NB501016:230:HFVJLAFXY:3:21608:18548:13709/1‑151 (MQ=7)
                                                                                  ATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCT                                                                      >  NB501016:230:HFVJLAFXY:4:11602:9680:8932/1‑151 (MQ=7)
                                                                                    CCGCGACAGCACGAAAGTACAGAATGCGGGTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGGCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGC                                                                    <  NB501016:230:HFVJLAFXY:4:11401:13572:3093/151‑1 (MQ=6)
                                                                                     CGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATA                                                                                                     <  NB501016:230:HFVJLAFXY:1:21104:16831:6965/117‑1 (MQ=8)
                                                                                      GCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCG                                                                  >  NB501016:230:HFVJLAFXY:4:21611:15073:18630/1‑151 (MQ=11)
                                                                                       CGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGC                                                                 <  NB501016:230:HFVJLAFXY:2:21302:14541:4509/151‑1 (MQ=15)
                                                                                           AGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTT                                                             <  NB501016:230:HFVJLAFXY:4:11502:20143:10141/151‑1 (MQ=19)
                                                                                            GCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTC                                                            >  NB501016:230:HFVJLAFXY:1:21310:2367:19673/1‑151 (MQ=19)
                                                                                            GCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTC                                                            >  NB501016:230:HFVJLAFXY:2:21212:13138:1896/1‑151 (MQ=19)
                                                                                            GCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTC                                                            >  NB501016:230:HFVJLAFXY:2:11204:10001:10709/1‑151 (MQ=19)
                                                                                            GCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCG                                                                                                              >  NB501016:230:HFVJLAFXY:1:21303:15448:17736/1‑101 (MQ=9)
                                                                                             CACGAAAGTACAGACTGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCT                                                           <  NB501016:230:HFVJLAFXY:1:11304:19032:1857/151‑1 (MQ=19)
                                                                                             CACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGT                                                              <  NB501016:230:HFVJLAFXY:4:21608:1582:14161/148‑1 (MQ=19)
                                                                                             CACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGGTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCT                                                           <  NB501016:230:HFVJLAFXY:3:11604:25815:14809/151‑1 (MQ=19)
                                                                                             CACGAAAGTACAGAATGCGGTTTCCACCACTTCAGAGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCT                                                           >  NB501016:230:HFVJLAFXY:1:21301:11095:4892/1‑151 (MQ=19)
                                                                                              ACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTT                                                          <  NB501016:230:HFVJLAFXY:3:11407:22224:10732/151‑1 (MQ=19)
                                                                                              ACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTT                                                          <  NB501016:230:HFVJLAFXY:1:11308:14997:12346/151‑1 (MQ=19)
                                                                                              ACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTT                                                          <  NB501016:230:HFVJLAFXY:4:11611:6464:3987/151‑1 (MQ=19)
                                                                                                GAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCA                                                        <  NB501016:230:HFVJLAFXY:4:21407:22422:2235/151‑1 (MQ=19)
                                                                                                GAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGATCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCA                                                        <  NB501016:230:HFVJLAFXY:1:21106:10888:12864/151‑1 (MQ=18)
                                                                                                GAAAGTACAGAATGCGGTGTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGATATCTGCCTCGCTGGCCTGCCGCAGTGCTTCA                                                        <  NB501016:230:HFVJLAFXY:1:21107:14573:17976/151‑1 (MQ=18)
                                                                                                 AAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGGTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAA                                                       <  NB501016:230:HFVJLAFXY:3:11511:21152:18327/151‑1 (MQ=19)
                                                                                                  AAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAAC                                                      <  NB501016:230:HFVJLAFXY:4:21410:7814:11325/151‑1 (MQ=19)
                                                                                                    GTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACcg                                                    >  NB501016:230:HFVJLAFXY:1:11203:23854:10771/1‑149 (MQ=17)
                                                                                                    GTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCA                                                                                                        <  NB501016:230:HFVJLAFXY:4:11510:18391:8737/99‑1 (MQ=9)
                                                                                                     TACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTGCTTCAA                                                       <  NB501016:230:HFVJLAFXY:4:21505:25016:16566/147‑1 (MQ=19)
                                                                                                      ACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTACGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATATGCATCGCTGCCCTGCCGCAGTTCTTCACCCTCC                                                  >  NB501016:230:HFVJLAFXY:2:11209:19422:13656/1‑151 (MQ=15)
                                                                                                      ACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCG                                                                                                                                     <  NB501016:230:HFVJLAFXY:3:21411:9961:6532/68‑1 (MQ=11)
                                                                                                       CAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGCGGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCC                                                 >  NB501016:230:HFVJLAFXY:1:11210:3654:10758/1‑151 (MQ=6)
                                                                                                       CAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCC                                                 >  NB501016:230:HFVJLAFXY:1:11306:17550:20192/1‑151 (MQ=6)
                                                                                                       CAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCA                                                        >  NB501016:230:HFVJLAFXY:3:11405:9527:11660/1‑144 (MQ=20)
                                                                                                       CAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATATGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCC                                                 >  NB501016:230:HFVJLAFXY:1:11202:18943:15934/1‑151 (MQ=6)
                                                                                                         gactggagtcagacgtgtgctcttccgaTCTCTGGCATTCTTCAGTTCCTGAGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGG                                               <  NB501016:230:HFVJLAFXY:2:11309:22039:5450/123‑1 (MQ=7)
                                                                                                         GAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGG                                               >  NB501016:230:HFVJLAFXY:3:21409:8376:6773/1‑151 (MQ=6)
                                                                                                         GAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGG                                               >  NB501016:230:HFVJLAFXY:1:21207:15761:12639/1‑151 (MQ=6)
                                                                                                         GAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCACTGGCCTGCCGCAGTTCTTCAACCTCCCGG                                               >  NB501016:230:HFVJLAFXY:2:21102:11737:7759/1‑151 (MQ=6)
                                                                                                          AATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAG                                                                                                    <  NB501016:230:HFVJLAFXY:3:11508:26224:13391/97‑1 (MQ=9)
                                                                                                           ATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTATTCAGTTCCTGTGCGTCGGCCTGCGCACTCGTAAGTCTATGGCGTTAGTAATCAATAGTTACTGGATGTAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCG                                             >  NB501016:230:HFVJLAFXY:2:11207:18745:18511/1‑151 (MQ=5)
                                                                                                            TGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTTCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGC                                            >  NB501016:230:HFVJLAFXY:3:21503:8345:12432/1‑151 (MQ=6)
                                                                                                             GCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGC                                            <  NB501016:230:HFVJLAFXY:3:11408:20323:17887/150‑1 (MQ=6)
                                                                                                              CGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGG                                               >  NB501016:230:HFVJLAFXY:4:21403:15409:11456/1‑146 (MQ=6)
                                                                                                               GGTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACC                                                     <  NB501016:230:HFVJLAFXY:3:21602:19202:4476/139‑1 (MQ=18)
                                                                                                                GTTTCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCT                                        <  NB501016:230:HFVJLAFXY:2:21303:17338:19045/151‑1 (MQ=6)
                                                                                                                   TCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTT                                      <  NB501016:230:HFVJLAFXY:4:21405:23188:18373/150‑1 (MQ=6)
                                                                                                                   TCCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCC                                                                   >  NB501016:230:HFVJLAFXY:4:11509:16174:4090/1‑121 (MQ=9)
                                                                                                                    CCACCCCTTCAGCGGAGTCTCTGGCATTCTTCCGGTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTGCGTACTCAATAGTTCCTGGCTGGAGAGCTGCCTCGCTGGCCTGCCGCAGTTCTTCACCCTCCCGGCGCAGCTGTTC                                    >  NB501016:230:HFVJLAFXY:2:11211:26384:18764/1‑151 (MQ=5)
                                                                                                                    CCACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTT                                     <  NB501016:230:HFVJLAFXY:1:11102:11982:2159/150‑1 (MQ=6)
                                                                                                                     CACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                                   >  NB501016:230:HFVJLAFXY:3:11512:2710:8106/1‑151 (MQ=6)
                                                                                                                     CACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                                   >  NB501016:230:HFVJLAFXY:4:11409:3687:17199/1‑151 (MQ=6)
                                                                                                                     CACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCAAGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTTGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCG                                   <  NB501016:230:HFVJLAFXY:3:21601:11730:6869/151‑1 (MQ=6)
                                                                                                                     CACCACTTCAGCGGAATCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATA                                                                                                     >  NB501016:230:HFVJLAFXY:4:21609:5052:15001/1‑85 (MQ=9)
                                                                                                                      ACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGT                                  <  NB501016:230:HFVJLAFXY:2:11301:20487:9947/151‑1 (MQ=6)
                                                                                                                      ACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGT                                  >  NB501016:230:HFVJLAFXY:4:21511:17655:16735/1‑151 (MQ=6)
                                                                                                                      ACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCACTTCTTCAACCTCCCGGCGCAGCTTTTCGT                                  >  NB501016:230:HFVJLAFXY:2:11111:5859:5578/1‑151 (MQ=6)
                                                                                                                      ACCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTG                                                                                  >  NB501016:230:HFVJLAFXY:4:21509:26586:10427/1‑103 (MQ=8)
                                                                                                                       CCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCACCCTCCCGGCGCAGCTTTTCGTT                                 >  NB501016:230:HFVJLAFXY:3:21505:18943:1369/1‑151 (MQ=6)
                                                                                                                       CCACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGGCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTT                                 <  NB501016:230:HFVJLAFXY:3:11510:14906:12915/151‑1 (MQ=6)
                                                                                                                        CACTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTC                                <  NB501016:230:HFVJLAFXY:4:21605:3966:12213/151‑1 (MQ=6)
                                                                                                                          CTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGCTCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCGC                              >  NB501016:230:HFVJLAFXY:1:21203:2329:18023/1‑151 (MQ=6)
                                                                                                                          CTTCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTC                              >  NB501016:230:HFVJLAFXY:2:21204:11442:13505/1‑151 (MQ=6)
                                                                                                                            TCAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTC                              <  NB501016:230:HFVJLAFXY:2:21309:18788:17784/149‑1 (MQ=6)
                                                                                                                             CAGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTCCTCAATAGTGCCTGGCTGGAGATCTGCCTCGCGGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTGTTCGTTCTCAAT                           >  NB501016:230:HFVJLAFXY:1:21205:16181:1317/1‑151 (MQ=6)
                                                                                                                              AGCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATT                          <  NB501016:230:HFVJLAFXY:1:11106:12804:19883/151‑1 (MQ=6)
                                                                                                                               GCGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTT                         >  NB501016:230:HFVJLAFXY:2:11305:18705:20168/1‑151 (MQ=6)
                                                                                                                                CGGAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTC                        <  NB501016:230:HFVJLAFXY:1:11209:11090:10597/151‑1 (MQ=6)
                                                                                                                                  GAGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                      <  NB501016:230:HFVJLAFXY:2:21304:11842:7684/151‑1 (MQ=6)
                                                                                                                                  GAGTCGCGGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCGGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                      <  NB501016:230:HFVJLAFXY:1:11311:25270:16850/151‑1 (MQ=6)
                                                                                                                                  GAGGCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGAGGGCGGTCGTCCTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAG                      <  NB501016:230:HFVJLAFXY:1:11112:25364:19313/151‑1 (MQ=5)
                                                                                                                                   AGTCTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCT                                                                           <  NB501016:230:HFVJLAFXY:4:21401:2910:15089/97‑1 (MQ=8)
                                                                                                                                      GTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                  >  NB501016:230:HFVJLAFXY:4:21502:11976:6504/1‑151 (MQ=6)
                                                                                                                                      CTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCCACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                  >  NB501016:230:HFVJLAFXY:4:11501:13068:19117/1‑151 (MQ=6)
                                                                                                                                      CTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                  >  NB501016:230:HFVJLAFXY:2:21202:2018:7547/1‑151 (MQ=6)
                                                                                                                                      CTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                  >  NB501016:230:HFVJLAFXY:3:11610:2480:9396/1‑151 (MQ=6)
                                                                                                                                      CTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                  >  NB501016:230:HFVJLAFXY:2:21210:10152:18443/1‑151 (MQ=6)
                                                                                                                                      CTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                  >  NB501016:230:HFVJLAFXY:3:21507:14663:10701/1‑151 (MQ=6)
                                                                                                                                      CTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATC                  >  NB501016:230:HFVJLAFXY:2:21305:11372:18324/1‑151 (MQ=6)
                                                                                                                                      CTCTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCACGGCGCAGCTTTACGATCTCAAATTCAGCATC                  >  NB501016:230:HFVJLAFXY:3:11609:1186:16379/1‑151 (MQ=5)
                                                                                                                                        CTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC                >  NB501016:230:HFVJLAFXY:2:21206:20857:10943/1‑151 (MQ=6)
                                                                                                                                        CTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC                >  NB501016:230:HFVJLAFXY:3:11507:23409:6682/1‑151 (MQ=6)
                                                                                                                                        CTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC                >  NB501016:230:HFVJLAFXY:3:21606:10628:4754/1‑151 (MQ=6)
                                                                                                                                        CTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCAT                   <  NB501016:230:HFVJLAFXY:2:11107:11037:6024/148‑1 (MQ=6)
                                                                                                                                        CTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGATCTCAATTTCAGCATCCC                >  NB501016:230:HFVJLAFXY:3:11505:16701:18524/1‑151 (MQ=6)
                                                                                                                                        CTGGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTT                                     <  NB501016:230:HFVJLAFXY:2:21110:12809:9440/130‑1 (MQ=7)
                                                                                                                                          GGCATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGCGGGCGGTCGTACTCAATAGTTCCTCGCGGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCAGCCC                <  NB501016:230:HFVJLAFXY:3:11605:7372:20333/149‑1 (MQ=5)
                                                                                                                                            CATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTC            <  NB501016:230:HFVJLAFXY:1:21309:14813:13737/151‑1 (MQ=32)
                                                                                                                                            CATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTC            >  NB501016:230:HFVJLAFXY:4:11507:3856:14598/1‑151 (MQ=6)
                                                                                                                                             ATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG           <  NB501016:230:HFVJLAFXY:3:21401:22162:3226/151‑1 (MQ=6)
                                                                                                                                             ATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTC            >  NB501016:230:HFVJLAFXY:1:11310:10809:10437/1‑150 (MQ=6)
                                                                                                                                             ATTCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATATGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG           >  NB501016:230:HFVJLAFXY:2:21205:2513:16942/1‑151 (MQ=6)
                                                                                                                                             ATTCTTCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCG           <  NB501016:230:HFVJLAFXY:3:11404:14418:2483/151‑1 (MQ=6)
                                                                                                                                               TCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC         >  NB501016:230:HFVJLAFXY:1:11101:10299:7719/1‑151 (MQ=7)
                                                                                                                                               TCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGG          <  NB501016:230:HFVJLAFXY:4:21508:19810:13837/150‑1 (MQ=7)
                                                                                                                                               TCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGG          <  NB501016:230:HFVJLAFXY:4:11505:16491:20022/150‑1 (MQ=7)
                                                                                                                                               TCTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGAGCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGG          <  NB501016:230:HFVJLAFXY:4:21512:6384:10222/150‑1 (MQ=6)
                                                                                                                                                CTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC         >  NB501016:230:HFVJLAFXY:4:11503:8056:17583/1‑150 (MQ=7)
                                                                                                                                                CTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCC‑TTCGGCAT       >  NB501016:230:HFVJLAFXY:4:21408:21181:2948/1‑151 (MQ=6)
                                                                                                                                                CTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTC                              <  NB501016:230:HFVJLAFXY:3:21407:12929:10618/129‑1 (MQ=7)
                                                                                                                                                CTTCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTACG                                   >  NB501016:230:HFVJLAFXY:1:11110:3707:1220/1‑124 (MQ=7)
                                                                                                                                                  TCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC         <  NB501016:230:HFVJLAFXY:3:11609:9632:5773/148‑1 (MQ=7)
                                                                                                                                                  TCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC         <  NB501016:230:HFVJLAFXY:4:21503:4606:3924/148‑1 (MQ=7)
                                                                                                                                                  TCAGTTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGC         <  NB501016:230:HFVJLAFXY:2:21103:25072:6501/148‑1 (MQ=7)
                                                                                                                                                  TCAGTTCCTGTGCGGCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTTCCTGGCTGGAGATCTGCCTCGCTGGCCTGCCGCAGTTCTTCAACCTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCAT       <  NB501016:230:HFVJLAFXY:1:21208:8425:4023/150‑1 (MQ=6)
                                                                                                                                                      |                                                                                                                                                      
AACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCTGCGCACGCGTAAGTCGATGGCGTTCGTACTCAATAGTCCCTGGCTGGAGATCTGTCTCGCTGGCCTGCCGCAGTTCTTCAACTTCCCGGCGCAGCTTTTCGTTCTCAATTTCAGCATCCCTTTCGGCATACCAT  >  NC_000913/1635924‑1636224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑