Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,636,455 G→A 90.4% intergenic (‑88/‑301) nohQ ← / → ynfO pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,636,4550GA90.4% 833.0 / 58.8 432intergenic (‑88/‑301)nohQ/ynfOpseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage
Reads supporting (aligned to +/- strand):  ref base G (20/21);  new base A (199/188);  total (221/211)
Fisher's exact test for biased strand distribution p-value = 8.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 434 total aligned reads displayed.

AATGGTACGGATACTCGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT  >  NC_000913/1636311‑1636602
                                                                                                                                                |                                                                                                                                                   
    gtccGGATACTCGCACCGAAACTGTCAGCCAGCGGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACCGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTAttt                                                                                                                                             <  6:78401/146‑1 (MQ=11)
                gCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAAc                                                                                                                                  <  7:251817/148‑1 (MQ=255)
                    cGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGTCAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATaaa                                                                                                                             >  1:341879/1‑149 (MQ=11)
                                       ctatttGTTCACTTCCATTGTTCATTCCACCGACAAAACCAGAGAAACGAAACGACAGAGGCCAACAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACgaagaagaa                                                                                                            <  6:363682/144‑1 (MQ=1)
                                       ctTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGATTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACg                                                                                                          <  1:78433/149‑1 (MQ=11)
                                            tgttgACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGaaa                                                                                                      <  2:89721/148‑1 (MQ=255)
                                                 aCTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGG‑CCAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCtt                                                                                                <  2:62553/148‑1 (MQ=11)
                                                       aTTGTTCATTCCACGGTCAAAAACAGAGAAAGGAAACGACATCTGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCg                                                                                          <  6:31553/149‑1 (MQ=11)
                                                         tGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCgg                                                                                         >  4:66417/1‑148 (MQ=255)
                                                           ttCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGaa                                                                                       >  8:59345/1‑148 (MQ=255)
                                                                     ggacAAAATCAGATAAATGAAACGACAGAGTCCAACAAGCTCGCTTTCAGAACCTGTCGTTTACTTTCTTTTCATAGGTTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCTTAAAATTTTCATaa                                                                            <  3:261272/146‑1 (MQ=255)
                                                                     ggCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATa                                                                             >  1:192156/1‑148 (MQ=255)
                                                                       ccAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAAt                                                                          <  2:21041/149‑1 (MQ=255)
                                                                                                      cAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTaa                                           <  2:16884/149‑1 (MQ=40)
                                                                                                      cAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTaa                                           >  2:128215/1‑149 (MQ=40)
                                                                                                      cAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTaa                                           >  8:166212/1‑149 (MQ=40)
                                                                                                       aaaaGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAAc                                          <  7:59345/149‑1 (MQ=39)
                                                                                                       aaaaGCCCGCTTTCAGCACATGTCGTTTCCTTTCTTTTCATAGTGTATTTAAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAAAGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAAc                                          <  1:241001/149‑1 (MQ=11)
                                                                                                             tCGCTTTCAGCACCTGTCGTTTCCATTCTTTTAAGAGGGGATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAAAGCCGTAAACCGGAAAATTTAAATAAATAGCGAAACCCCGCGAGGTCGCCGCCCCGTAACCTGTCg                                    >  2:183564/1‑149 (MQ=11)
                                                                                                              cgctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACgaagaa                                                                                                               <  8:353955/70‑1 (MQ=1)
                                                                                                              cgctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCg                                                                                          >  3:324325/4‑94 (MQ=1)
                                                                                                              cgctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCg                                                                                          <  4:324325/91‑1 (MQ=1)
                                                                                                               gctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCtt                                                                                                <  4:119251/85‑1 (MQ=11)
                                                                                                               gctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATa                                                                         <  3:200071/108‑1 (MQ=11)
                                                                                                               gctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGCGGTCGCCGCCCCGTAACCTGGCggg                                  >  2:74942/3‑148 (MQ=11)
                                                                                                                 tttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCTAAAACCCGCCAGGTcgcc                                                    >  7:110790/1‑129 (MQ=11)
                                                                                                                 tttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGAt                                 >  8:79926/1‑148 (MQ=12)
                                                                                                                 tttAAGCAAATGTCGTTTCCTTTCTTTTAACAGATTATTTTAAATAAAAACATTAAATTAAGACCAACAACAACTGAAACGCCTTAAAACCAAAAAATTTCATAAATAAATAAAACCCGCGAGCACGCCGCACCGAACCCTGACGGAt                                 >  4:172490/1‑148 (MQ=255)
                                                                                                                     aGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGTAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCg                            <  1:277409/149‑1 (MQ=255)
                                                                                                                       cACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCgg                           >  6:172808/1‑148 (MQ=39)
                                                                                                                       cACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                 <  6:40760/126‑1 (MQ=12)
                                                                                                                       cACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                 >  6:377356/1‑126 (MQ=12)
                                                                                                                       cACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCgg                           >  7:390000/1‑148 (MQ=255)
                                                                                                                       cACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGATATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCgg                           >  2:374041/1‑148 (MQ=255)
                                                                                                                        aCCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGTAGAAGAACGGAAAAGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCTGa                          >  1:109354/1‑148 (MQ=11)
                                                                                                                        aCCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGa                          >  4:235727/1‑148 (MQ=255)
                                                                                                                         ccTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCtt                                                                                                <  6:118161/77‑1 (MQ=11)
                                                                                                                         ccTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCTCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGaaa                        <  7:278343/149‑1 (MQ=255)
                                                                                                                          cTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCg                                                                                          <  7:96576/82‑1 (MQ=11)
                                                                                                                          cTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                       >  5:52222/1‑149 (MQ=255)
                                                                                                                          cTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                       <  5:251632/149‑1 (MQ=255)
                                                                                                                          cTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                       <  7:68952/149‑1 (MQ=255)
                                                                                                                          cTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                       <  1:153340/149‑1 (MQ=255)
                                                                                                                          cTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                       <  3:191568/149‑1 (MQ=255)
                                                                                                                          cTGTCGTTTCCTTTCTGTTCAGAGGGTATTTTAAATAAAAACATTAACTTATGCCGAAGAAGAACTGACACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTTTCGGATCACCGGAAAg                       >  8:300641/1‑149 (MQ=11)
                                                                                                                           tGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGaaa                        >  6:275762/1‑147 (MQ=39)
                                                                                                                           tGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTGATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                       >  5:176781/1‑148 (MQ=255)
                                                                                                                           tGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                 <  1:163865/122‑1 (MQ=12)
                                                                                                                           tGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                       >  7:133214/1‑148 (MQ=255)
                                                                                                                            gTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                 <  2:34238/121‑1 (MQ=12)
                                                                                                                             tCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATaaa                                                                           >  4:31686/1‑94 (MQ=11)
                                                                                                                             tCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGa                     <  3:168949/148‑1 (MQ=255)
                                                                                                                             tCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAc                    >  5:369106/1‑149 (MQ=255)
                                                                                                                              cGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAAttt                                                                                  >  1:38813/1‑86 (MQ=11)
                                                                                                                              cGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAAttt                                                                                  <  2:38813/86‑1 (MQ=255)
                                                                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGaaac                                                                                                     >  5:119194/1‑65 (MQ=255)
                                                                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACTCCTTAAACGGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                  <  2:331886/149‑1 (MQ=11)
                                                                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCg                                                                                          <  8:264129/77‑1 (MQ=255)
                                                                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAAccc                                                               <  5:382618/104‑1 (MQ=255)
                                                                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                  <  3:73017/149‑1 (MQ=255)
                                                                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                  <  4:268993/149‑1 (MQ=255)
                                                                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                  >  5:319627/1‑149 (MQ=255)
                                                                                                                               gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAcc                   <  3:20501/148‑1 (MQ=255)
                                                                                                                               gTTTCCTTTATTTTCAGAGTGTATTTTAAATAAACACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                  <  6:210971/149‑1 (MQ=11)
                                                                                                                                tttCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCg                 >  7:211387/1‑149 (MQ=255)
                                                                                                                                 ttCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCg                 >  5:277121/1‑148 (MQ=255)
                                                                                                                                   ccTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAc                                                                                                           <  8:118889/56‑1 (MQ=255)
                                                                                                                                   ccTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAcc                                                                                           >  4:215852/1‑72 (MQ=255)
                                                                                                                                   ccTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTa               <  7:309077/148‑1 (MQ=255)
                                                                                                                                   ccTTTCTTTTCAGAGGGTAATTTAAATAAAAACATTAAGTTATGACGAAGAAGAACCGAACAGCCTTAAACCGGAAAATTTTCATAAATAGAGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTa               >  1:32136/1‑148 (MQ=14)
                                                                                                                                    ctttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTa               >  3:308352/1‑147 (MQ=255)
                                                                                                                                    ctttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAcc                                                                                           <  8:202781/71‑1 (MQ=255)
                                                                                                                                    ctttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTc                                                                                >  6:248134/1‑82 (MQ=11)
                                                                                                                                    ctttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCcgc                                                             >  1:58469/1‑101 (MQ=255)
                                                                                                                                    ctttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTa               >  8:375270/1‑147 (MQ=255)
                                                                                                                                     tttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCTCCGCCCCGTAACCTGTCGGATCACCGGAAAGGa                     >  1:178432/1‑140 (MQ=255)
                                                                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTc                                                                                >  2:160866/1‑81 (MQ=255)
                                                                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGt                                      <  5:195630/123‑1 (MQ=12)
                                                                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGt                                      >  6:195630/1‑123 (MQ=12)
                                                                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg            >  3:118467/1‑149 (MQ=255)
                                                                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg            >  6:259016/1‑149 (MQ=255)
                                                                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg            >  7:173189/1‑149 (MQ=255)
                                                                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg            >  8:3186/1‑149 (MQ=255)
                                                                                                                                     tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg            >  6:323780/1‑149 (MQ=255)
                                                                                                                                      ttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAACCCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACAGGAAAGGACCCGTAAAGt           >  6:134858/1‑149 (MQ=14)
                                                                                                                                      ttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt           >  4:360349/1‑149 (MQ=255)
                                                                                                                                       tctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACTAAGAAGAACGGACACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGAAAAGt           <  3:60491/148‑1 (MQ=11)
                                                                                                                                       tctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt           <  3:235727/148‑1 (MQ=255)
                                                                                                                                       tctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt           <  1:379556/148‑1 (MQ=255)
                                                                                                                                        ctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGc                                                                       >  4:188310/1‑87 (MQ=11)
                                                                                                                                        ctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAgg                                                         >  5:356711/1‑101 (MQ=255)
                                                                                                                                        ctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                 <  1:357403/109‑1 (MQ=255)
                                                                                                                                         ttttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGa         >  1:98883/1‑148 (MQ=255)
                                                                                                                                         ttttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGa         >  7:368459/1‑148 (MQ=255)
                                                                                                                                         ttttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTATCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCCTAAAGTGa         >  4:208809/1‑148 (MQ=14)
                                                                                                                                           ttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATa       <  5:157830/148‑1 (MQ=255)
                                                                                                                                           ttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATa       <  2:291567/148‑1 (MQ=255)
                                                                                                                                            tCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATaa      <  5:207692/148‑1 (MQ=255)
                                                                                                                                             cAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGt                                                        <  6:223757/97‑1 (MQ=255)
                                                                                                                                             cAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGt                                                        >  5:223757/1‑97 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
AATGGTACGGATACTCGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT  >  NC_000913/1636311‑1636602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 566 total aligned reads displayed.
TCTGAATGGTACGGATACTCGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTAT  >  NC_000913/1636307‑1636605
                                                                                                                                                    |                                                                                                                                                      
TCTTAATGGTACTGATACTCGCACCTAAAATGTCAGCCTGCTGGTTTTTGTTGACTTCCATTGTTCATTCCACTTCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGG                                                                                                                                                       <  NB501016:230:HFVJLAFXY:4:11603:17044:10699/150‑1 (MQ=45)
        GTACGGATACTCGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGG‑CCAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTA                                                                                                                                              >  NB501016:230:HFVJLAFXY:1:11111:1163:8171/1‑150 (MQ=0)
        GTACGGATACTCGAACCGAAAATGTCAGACAGCTGCTTTTTGTTTCCTTCCATAGTTCATTCCACGGCCAAAAACAGAGAACGGAAACGACAGAGGCCAAAACGCTCGCTTTCAGCACCTCTCGTTTCCGTTCTTTTCCGAGGGTATTTT                                                                                                                                               >  NB501016:230:HFVJLAFXY:3:11404:8808:10615/1‑150 (MQ=5)
                   CGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACAT                                                                                                                                   >  NB501016:230:HFVJLAFXY:4:11611:25133:15276/1‑151 (MQ=0)
                   CGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACAT                                                                                                                                   <  NB501016:230:HFVJLAFXY:1:11305:23405:10854/151‑1 (MQ=0)
                   CGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTACTTTCTTTTCAGAGGGTATTTTAAATAAAAACAT                                                                                                                                   >  NB501016:230:HFVJLAFXY:4:21508:17509:9408/1‑151 (MQ=0)
                    GCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGATGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATT                                                                                                                                  <  NB501016:230:HFVJLAFXY:3:11506:12449:2012/151‑1 (MQ=0)
                    GCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATT                                                                                                                                  <  NB501016:230:HFVJLAFXY:4:11512:22615:10682/151‑1 (MQ=0)
                    GCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATT                                                                                                                                  >  NB501016:230:HFVJLAFXY:1:21107:6431:5010/1‑151 (MQ=0)
                     CACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTA                                                                                                                                 <  NB501016:230:HFVJLAFXY:3:21405:24467:13031/151‑1 (MQ=0)
                     CACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTA                                                                                                                                 <  NB501016:230:HFVJLAFXY:1:11305:15736:14724/151‑1 (MQ=0)
                     CACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTA                                                                                                                                 <  NB501016:230:HFVJLAFXY:2:21310:12775:10183/151‑1 (MQ=0)
                     CACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTA                                                                                                                                 <  NB501016:230:HFVJLAFXY:3:11501:17119:18005/151‑1 (MQ=0)
                     CACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATT                                                                                                                                  >  NB501016:230:HFVJLAFXY:1:21310:8671:2228/1‑150 (MQ=0)
                     CACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATT                                                                                                                                  >  NB501016:230:HFVJLAFXY:1:21104:1337:12501/1‑150 (MQ=0)
                     CACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATT                                                                                                                                  >  NB501016:230:HFVJLAFXY:1:11201:20030:4081/1‑150 (MQ=0)
                     CACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATT                                                                                                                                  >  NB501016:230:HFVJLAFXY:2:11208:21918:11618/1‑150 (MQ=0)
                     CACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATT                                                                                                                                  >  NB501016:230:HFVJLAFXY:2:11109:26641:10651/1‑150 (MQ=0)
                      ACCGAAAATGTCAGCCATCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGTACAAAAACAGAGTATGGAATCGAAAGAGGCCAAAAAGCTCGATTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGTTATTTTAAATAAAAACATTAA                                                                                                                                <  NB501016:230:HFVJLAFXY:1:21309:16019:19183/151‑1 (MQ=5)
                      ACCGAAAATGTCAGCCATATTCTTTTTGTTGACTTCCATTGTTCAATCCACGGACAAAAACAGAGAAATGAAACGACAGAGGCCAAAAATCTCTCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAA                                                                                                                                <  NB501016:230:HFVJLAFXY:3:11411:10111:9682/151‑1 (MQ=0)
                      ACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAA                                                                                                                                <  NB501016:230:HFVJLAFXY:2:11212:26096:8068/151‑1 (MQ=0)
                      ACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTA                                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11501:20175:7206/1‑150 (MQ=0)
                       CCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGTCAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATaaat                                                                                                                               >  NB501016:230:HFVJLAFXY:1:21302:14508:14741/1‑147 (MQ=7)
                       CCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGGGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAG                                                                                                                               <  NB501016:230:HFVJLAFXY:1:11107:12937:16472/151‑1 (MQ=0)
                       CCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAG                                                                                                                               <  NB501016:230:HFVJLAFXY:3:11604:19991:13192/151‑1 (MQ=0)
                       CCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAG                                                                                                                               <  NB501016:230:HFVJLAFXY:1:21308:17758:18149/151‑1 (MQ=0)
                       CCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAG                                                                                                                               <  NB501016:230:HFVJLAFXY:3:11611:23626:17328/151‑1 (MQ=0)
                       CCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTA                                                                                                                                 >  NB501016:230:HFVJLAFXY:3:11409:8452:11996/1‑149 (MQ=0)
                       CCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTACTTTTCAGAGGGTATTTTAAATAAAAACATTA                                                                                                                                 >  NB501016:230:HFVJLAFXY:4:21407:7208:10000/1‑149 (MQ=0)
                        CGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGT                                                                                                                              <  NB501016:230:HFVJLAFXY:4:11410:19416:11031/151‑1 (MQ=0)
                        CGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGT                                                                                                                              <  NB501016:230:HFVJLAFXY:1:21211:5038:12647/151‑1 (MQ=0)
                        CGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGT                                                                                                                              <  NB501016:230:HFVJLAFXY:2:21201:5021:5499/151‑1 (MQ=0)
                        CGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGT                                                                                                                              >  NB501016:230:HFVJLAFXY:4:21607:11103:11010/1‑151 (MQ=0)
                        CGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGT                                                                                                                              >  NB501016:230:HFVJLAFXY:3:11607:18533:13193/1‑151 (MQ=0)
                         GAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTT                                                                                                                             <  NB501016:230:HFVJLAFXY:3:11407:4741:6460/151‑1 (MQ=0)
                         GAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTT                                                                                                                             <  NB501016:230:HFVJLAFXY:1:21305:3369:7003/151‑1 (MQ=0)
                          AAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTA                                                                                                                            <  NB501016:230:HFVJLAFXY:2:11312:24237:14444/151‑1 (MQ=0)
                          AAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTA                                                                                                                            <  NB501016:230:HFVJLAFXY:1:21312:14960:2048/151‑1 (MQ=0)
                          AAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTT                                                                                                                             >  NB501016:230:HFVJLAFXY:2:21303:6937:17306/1‑150 (MQ=0)
                          AAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGGCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTA                                                                                                                            <  NB501016:230:HFVJLAFXY:2:21209:23572:2737/151‑1 (MQ=0)
                           AAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTAT                                                                                                                           <  NB501016:230:HFVJLAFXY:2:11103:12886:16037/151‑1 (MQ=0)
                           AAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTAT                                                                                                                           <  NB501016:230:HFVJLAFXY:4:11405:1850:13707/151‑1 (MQ=0)
                           AAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTAT                                                                                                                           <  NB501016:230:HFVJLAFXY:1:11101:14060:4846/151‑1 (MQ=0)
                            AATGTCAGCCAGCTGCTTTTTTTTTACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACTAAAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTAGTTTCCTTTCTTTTCATAGTGTATTTTAAATAAAAACATTAAGTTATG                                                                                                                          <  NB501016:230:HFVJLAFXY:2:11306:23366:1677/151‑1 (MQ=0)
                            AATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATG                                                                                                                          <  NB501016:230:HFVJLAFXY:3:21503:23443:8195/151‑1 (MQ=0)
                            AATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATG                                                                                                                          <  NB501016:230:HFVJLAFXY:2:11108:5639:17588/151‑1 (MQ=0)
                            AATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATG                                                                                                                          <  NB501016:230:HFVJLAFXY:1:11203:15137:15958/151‑1 (MQ=0)
                             ATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGA                                                                                                                         <  NB501016:230:HFVJLAFXY:4:11504:16175:5332/151‑1 (MQ=0)
                             ATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGCGTATTTTAAATAAAAACATTAAGTTATG                                                                                                                          >  NB501016:230:HFVJLAFXY:3:21506:16694:9069/1‑150 (MQ=0)
                               GTCAGCCTGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGAC                                                                                                                        <  NB501016:230:HFVJLAFXY:1:11208:7913:17428/150‑1 (MQ=0)
                               GTCAGCCAGCTGCTTTTTGTTGACTTCGATTGTTCATTCCACGGTCTAAATCTGAGAAAGGAAACTACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTTTTTTAAATAAAATCATTAAGTTATGACG                                                                                                                       <  NB501016:230:HFVJLAFXY:1:21110:4186:20000/151‑1 (MQ=5)
                               GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACG                                                                                                                       >  NB501016:230:HFVJLAFXY:1:21306:14890:17251/1‑151 (MQ=60)
                               GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGTAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACG                                                                                                                       <  NB501016:230:HFVJLAFXY:1:21104:4245:12397/151‑1 (MQ=0)
                               GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACG                                                                                                                       <  NB501016:230:HFVJLAFXY:2:21101:18028:10620/151‑1 (MQ=0)
                               GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGAC                                                                                                                        <  NB501016:230:HFVJLAFXY:2:21209:8548:16611/150‑1 (MQ=0)
                               GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGAC                                                                                                                        <  NB501016:230:HFVJLAFXY:3:11505:17400:13850/150‑1 (MQ=0)
                               GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGAC                                                                                                                        <  NB501016:230:HFVJLAFXY:3:21508:6631:15538/150‑1 (MQ=0)
                                 CAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAA                                                                                                                     <  NB501016:230:HFVJLAFXY:4:11402:21458:10677/151‑1 (MQ=0)
                                 CAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTACTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                      >  NB501016:230:HFVJLAFXY:2:11201:15996:1733/1‑150 (MQ=0)
                                  AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAATGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGACGCTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                      >  NB501016:230:HFVJLAFXY:4:21512:19521:3124/1‑149 (MQ=0)
                                  AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAATAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                    <  NB501016:230:HFVJLAFXY:2:21207:10770:4121/151‑1 (MQ=0)
                                  AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11508:14666:10525/151‑1 (MQ=0)
                                  AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11611:12555:11292/151‑1 (MQ=0)
                                  AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                    <  NB501016:230:HFVJLAFXY:3:21507:2600:6844/151‑1 (MQ=0)
                                  AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGG‑CCAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                      >  NB501016:230:HFVJLAFXY:4:11411:23858:10956/1‑148 (MQ=6)
                                   GCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                      >  NB501016:230:HFVJLAFXY:1:21112:15163:8091/1‑148 (MQ=0)
                                   GCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                      >  NB501016:230:HFVJLAFXY:4:11605:20142:10792/1‑148 (MQ=0)
                                   GCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                      >  NB501016:230:HFVJLAFXY:4:21503:7175:1748/1‑148 (MQ=0)
                                    CCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGA                                                                                                                   >  NB501016:230:HFVJLAFXY:3:11512:10946:8718/1‑150 (MQ=0)
                                     CAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAG                                                                                                                 <  NB501016:230:HFVJLAFXY:3:11406:4380:19331/151‑1 (MQ=0)
                                        CTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                              >  NB501016:230:HFVJLAFXY:4:11403:17469:2553/1‑151 (MQ=0)
                                         TGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                             >  NB501016:230:HFVJLAFXY:4:21605:9220:2788/1‑151 (MQ=0)
                                          ggtttttggtgtcttacattgttcattcctcggagaaatagagagaatggaaaatacagaggccaaaaacctcgctttgtgctCCTGTGGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGATGAAGAACG                                                                                                             <  NB501016:230:HFVJLAFXY:4:11602:26457:2721/67‑1 (MQ=60)
                                          GCTTTTTGTTGACTTCCATTTTTCTTTCCACGGACTAATACAGAGATAGGAAACGACAGTGGCCAAAAAGCTCTATTTGATCAGCTGTTGTTTCCTTTCTTTTCAGAGGGTATTTTATATTATAACATTAAGTTATGACGAAGAAGttcgt                                                                                                            <  NB501016:230:HFVJLAFXY:1:11303:10533:1445/151‑6 (MQ=3)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACTGACAAAAACAGAGAAAGGAAACTACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:2:21306:2491:1098/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACTGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:4:21610:14775:14970/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCTCCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:1:21103:11023:3053/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTGAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:4:21409:22627:16140/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:4:11601:4989:10825/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:4:21510:4344:14348/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:2:11206:14797:6821/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:2:21305:11252:17611/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:2:21105:5827:4739/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:1:11204:21556:5762/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:4:21505:14539:14374/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:4:21604:23813:16352/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:2:11309:4170:8441/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:1:11104:18309:13439/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:2:11302:2233:9948/151‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                             <  NB501016:230:HFVJLAFXY:1:11309:13188:18826/150‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                             <  NB501016:230:HFVJLAFXY:1:11306:18653:12279/150‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                              <  NB501016:230:HFVJLAFXY:1:11302:17518:1307/149‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                              <  NB501016:230:HFVJLAFXY:4:11401:22774:12138/149‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                              <  NB501016:230:HFVJLAFXY:1:11106:22819:3084/149‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                              <  NB501016:230:HFVJLAFXY:4:11607:18391:4818/149‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                              <  NB501016:230:HFVJLAFXY:2:11211:25214:17042/149‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                              <  NB501016:230:HFVJLAFXY:2:11101:8317:9266/149‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                              <  NB501016:230:HFVJLAFXY:4:21406:17829:15894/149‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                              <  NB501016:230:HFVJLAFXY:2:11203:3984:15445/149‑1 (MQ=0)
                                          GCTTTTTGTTGACTTCCATATTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTAGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGTGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                             <  NB501016:230:HFVJLAFXY:2:21106:7813:1512/150‑1 (MQ=0)
                                           CTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            >  NB501016:230:HFVJLAFXY:3:21411:2465:17003/1‑150 (MQ=0)
                                            TTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGA                                                                                                           >  NB501016:230:HFVJLAFXY:2:21112:3310:11557/1‑150 (MQ=0)
                                              TTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGTAACGACTGATGCCAAAAAGCTCGCTTTCAGCACCTTTCGTTTCGTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAA                                                                                                         <  NB501016:230:HFVJLAFXY:2:11305:10012:12265/150‑1 (MQ=0)
                                                 GTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            <  NB501016:230:HFVJLAFXY:2:21104:10545:3330/144‑1 (MQ=0)
                                                   ttagTTCCATTGTTCATTCGAAGGACAATAACAGATCAAGGAAACGACCTAGGCCAAAAAGCTCGATTTAAGAACCTGTCTTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCT                                                                                                    <  NB501016:230:HFVJLAFXY:3:21402:26367:3974/146‑1 (MQ=0)
                                                   TGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTT                                                                                                   >  NB501016:230:HFVJLAFXY:4:21603:17082:6382/1‑151 (MQ=0)
                                                    GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                  <  NB501016:230:HFVJLAFXY:2:21104:4168:16939/151‑1 (MQ=0)
                                                    GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                  <  NB501016:230:HFVJLAFXY:4:11509:5250:15810/151‑1 (MQ=0)
                                                    GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                  <  NB501016:230:HFVJLAFXY:2:11301:19835:1617/151‑1 (MQ=0)
                                                    GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTT                                                                                                   <  NB501016:230:HFVJLAFXY:1:11212:10853:19086/150‑1 (MQ=0)
                                                    GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTT                                                                                                   <  NB501016:230:HFVJLAFXY:2:21110:8572:14899/150‑1 (MQ=0)
                                                      CTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGGGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAA                                                                                                 >  NB501016:230:HFVJLAFXY:4:21401:5335:19164/1‑150 (MQ=0)
                                                      CTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAA                                                                                                 >  NB501016:230:HFVJLAFXY:3:11509:17011:18652/1‑150 (MQ=0)
                                                      CTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTGTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGACCGGAAACGCCTTAA                                                                                                 >  NB501016:230:HFVJLAFXY:1:11209:3661:2194/1‑150 (MQ=0)
                                                      CTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTATTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAA                                                                                                 >  NB501016:230:HFVJLAFXY:4:11609:1958:17822/1‑150 (MQ=0)
                                                       TTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAC                                                                                               >  NB501016:230:HFVJLAFXY:1:11103:12304:6506/1‑151 (MQ=0)
                                                                    TCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTC                                                                                   >  NB501016:230:HFVJLAFXY:1:11104:6905:6736/1‑150 (MQ=60)
                                                                                              actacagatgacaaaaagatctcTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGTGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGC                                                        <  NB501016:230:HFVJLAFXY:3:11407:17309:8504/128‑1 (MQ=3)
                                                                                                          AAAAAGCCCGCTTTCAGCACATGTCGTTTCCTTTCTTTTCATAGTGTATTTAAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAAAGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACC                                            <  NB501016:230:HFVJLAFXY:1:21107:1111:17550/151‑1 (MQ=4)
                                                                                                           AAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCAGCGAGGTCGCCGCCCCGTAAGCT                                           >  NB501016:230:HFVJLAFXY:3:11503:12326:16760/1‑151 (MQ=0)
                                                                                                             AAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGT                                         <  NB501016:230:HFVJLAFXY:1:11311:6464:17909/151‑1 (MQ=0)
                                                                                                              AGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAATCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGAGAGGTCGCCGCCC                                                   >  NB501016:230:HFVJLAFXY:2:11204:15991:10049/1‑140 (MQ=0)
                                                                                                              AGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTC                                        >  NB501016:230:HFVJLAFXY:2:21308:23388:5835/1‑151 (MQ=0)
                                                                                                               GCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                   >  NB501016:230:HFVJLAFXY:4:21405:18965:19640/1‑139 (MQ=0)
                                                                                                                 TCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGG                                                                                            >  NB501016:230:HFVJLAFXY:2:21304:21370:13473/1‑96 (MQ=0)
                                                                                                                  CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAG                                                                           <  NB501016:230:HFVJLAFXY:2:21103:2992:12538/112‑1 (MQ=1)
                                                                                                                  CGCTTTCAGCACCAGTCGTTTCCTTTCTTTTCAGAGGGTAATTTAAATAAAAACAATAAGTTATGAAGAAGAAGAACGGAAACGCCTTAAACCGTAAAATTATCAAATCTAGCGAAAACCCTCGAGGTCGCCGCCCAGTAACATGTCGGA                                     >  NB501016:230:HFVJLAFXY:3:21603:4553:9691/1‑150 (MQ=0)
                                                                                                                    CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGAGAGGTCG                                                         >  NB501016:230:HFVJLAFXY:4:11411:9049:1480/1‑128 (MQ=5)
                                                                                                                    CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCG                                                                 <  NB501016:230:HFVJLAFXY:2:11209:19579:5798/120‑1 (MQ=5)
                                                                                                                    CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAAACCGCGAGGTCGCCTCCCAGTAACCTGTCGGATC                                   >  NB501016:230:HFVJLAFXY:2:11105:10581:2355/1‑150 (MQ=4)
                                                                                                                       TCAGCACCTGTCGTTTCCTTTCTTCTCCGAGGGTATAGTAAATAAACACATTAAGGTATGACGAACAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCTCCGCCCCGCAACCTGTCGGATCACC                                <  NB501016:230:HFVJLAFXY:3:21412:5296:3505/150‑1 (MQ=2)
                                                                                                                        CAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCTGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGGAACCGGTCGGATCACCGG                              >  NB501016:230:HFVJLAFXY:3:21609:26734:6443/1‑151 (MQ=18)
                                                                                                                        CAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGG                              <  NB501016:230:HFVJLAFXY:1:11106:2020:19163/151‑1 (MQ=20)
                                                                                                                        CAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGC                                                     <  NB501016:230:HFVJLAFXY:1:11307:15547:6096/128‑1 (MQ=7)
                                                                                                                          GCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                   >  NB501016:230:HFVJLAFXY:3:11408:25445:18634/1‑128 (MQ=7)
                                                                                                                           CACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGTAGAAGAACGGAAAAGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCTGAA                            >  NB501016:230:HFVJLAFXY:1:11208:13273:5058/1‑150 (MQ=25)
                                                                                                                            ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCTCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                          <  NB501016:230:HFVJLAFXY:4:21502:9826:7895/151‑1 (MQ=19)
                                                                                                                            ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                          <  NB501016:230:HFVJLAFXY:2:21108:13600:12436/151‑1 (MQ=20)
                                                                                                                            ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                          <  NB501016:230:HFVJLAFXY:2:11310:2724:10470/151‑1 (MQ=20)
                                                                                                                            ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                          <  NB501016:230:HFVJLAFXY:2:21103:13980:7389/151‑1 (MQ=20)
                                                                                                                            ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                          <  NB501016:230:HFVJLAFXY:4:11502:24439:13116/151‑1 (MQ=20)
                                                                                                                            ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                  >  NB501016:230:HFVJLAFXY:3:11511:5503:11775/1‑79 (MQ=9)
                                                                                                                             CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATATCGAAAACCCGCGCGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                         >  NB501016:230:HFVJLAFXY:1:11308:21985:19921/1‑151 (MQ=19)
                                                                                                                             CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                         <  NB501016:230:HFVJLAFXY:3:21411:21855:14386/151‑1 (MQ=20)
                                                                                                                             CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGG                                                                                            <  NB501016:230:HFVJLAFXY:4:11505:25813:6974/84‑1 (MQ=9)
                                                                                                                              CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTGATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                         >  NB501016:230:HFVJLAFXY:3:11609:10790:13583/1‑150 (MQ=19)
                                                                                                                              CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                         >  NB501016:230:HFVJLAFXY:4:11511:11308:17091/1‑150 (MQ=20)
                                                                                                                              CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                         >  NB501016:230:HFVJLAFXY:4:21507:11592:16204/1‑150 (MQ=20)
                                                                                                                              CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                         >  NB501016:230:HFVJLAFXY:4:11406:11555:1528/1‑150 (MQ=20)
                                                                                                                              CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                          <  NB501016:230:HFVJLAFXY:3:21511:22950:4006/149‑1 (MQ=20)
                                                                                                                               TGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCagatcggaagagcacacgtctggaactccagtc                       >  NB501016:230:HFVJLAFXY:2:11103:9836:9392/1‑118 (MQ=8)
                                                                                                                               TGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                       >  NB501016:230:HFVJLAFXY:3:11511:21814:19897/1‑151 (MQ=20)
                                                                                                                               TGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCG                                                                 <  NB501016:230:HFVJLAFXY:3:21505:11699:16721/109‑1 (MQ=8)
                                                                                                                                ccgctcTCCTTTCTTTTCAGGGGGTATTTTAACTAAAAACATTAAGTTACGACGAAGACGACCGGAAATGCCTTAAACCGGAAACTTTTCATAAATCGCGAAACCCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                       <  NB501016:230:HFVJLAFXY:1:21205:1566:2891/144‑1 (MQ=37)
                                                                                                                                GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACC                      <  NB501016:230:HFVJLAFXY:4:11404:4927:19276/151‑1 (MQ=20)
                                                                                                                                GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACC                      >  NB501016:230:HFVJLAFXY:3:21610:6252:5071/1‑151 (MQ=20)
                                                                                                                                GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACC                      <  NB501016:230:HFVJLAFXY:1:21209:20004:1849/151‑1 (MQ=20)
                                                                                                                                GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                       <  NB501016:230:HFVJLAFXY:4:21611:14472:13692/150‑1 (MQ=20)
                                                                                                                                GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                       <  NB501016:230:HFVJLAFXY:1:11110:14621:12426/150‑1 (MQ=20)
                                                                                                                                  CGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGCACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCGGCGAGGTGGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCG                    <  NB501016:230:HFVJLAFXY:1:11304:6998:13283/151‑1 (MQ=20)
                                                                                                                                  CGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGG                                                                                            >  NB501016:230:HFVJLAFXY:4:21411:14074:7617/1‑79 (MQ=9)
                                                                                                                                   GTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCAT                   >  NB501016:230:HFVJLAFXY:2:11302:11004:6397/1‑151 (MQ=18)
                                                                                                                                     TTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCG                                                                         >  NB501016:230:HFVJLAFXY:1:11110:5797:5416/1‑95 (MQ=8)
                                                                                                                                      TCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAACAACGGAAACGCCTGAAACCGGAAAATTTTCAAAAATAGCGAAAAACCGCGAGGTCGCCGCCCCGAAACCTGTCGCATCACAGGAAAGCACCCGTAA                 >  NB501016:230:HFVJLAFXY:2:21203:1869:2761/1‑150 (MQ=19)
                                                                                                                                      TCCTTTCTTTTCAGAGGGTAATTTAAATAAAAACATTAAGTTATGACGAAGAAGAACCGAACAGCCTTAAACCGGAAAATTTTCATAAATAGAGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAA                 >  NB501016:230:HFVJLAFXY:1:11106:1844:3234/1‑150 (MQ=34)
                                                                                                                                       CCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAG               <  NB501016:230:HFVJLAFXY:2:21301:25669:17328/151‑1 (MQ=32)
                                                                                                                                        CTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCTCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                       >  NB501016:230:HFVJLAFXY:1:11309:18072:4216/1‑142 (MQ=43)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT              >  NB501016:230:HFVJLAFXY:1:11310:19805:2697/1‑151 (MQ=34)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT              >  NB501016:230:HFVJLAFXY:1:11310:9249:13269/1‑151 (MQ=34)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT              >  NB501016:230:HFVJLAFXY:2:11201:8906:10821/1‑151 (MQ=34)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT              >  NB501016:230:HFVJLAFXY:2:21101:8371:10568/1‑151 (MQ=34)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACACGTAAAGT              >  NB501016:230:HFVJLAFXY:3:21409:3090:2576/1‑151 (MQ=27)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGG                              >  NB501016:230:HFVJLAFXY:3:21612:5594:5444/1‑135 (MQ=21)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTC                                        <  NB501016:230:HFVJLAFXY:3:11612:18667:20312/125‑1 (MQ=8)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                   >  NB501016:230:HFVJLAFXY:4:21608:15537:3241/1‑114 (MQ=8)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAG                                                             >  NB501016:230:HFVJLAFXY:1:21201:14382:11604/1‑104 (MQ=8)
                                                                                                                                        CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAACGGAAAATTTTCATAAATAGCGAAAAACCGCGAGGTCGCCGC                                                     >  NB501016:230:HFVJLAFXY:3:11608:10561:19971/1‑112 (MQ=7)
                                                                                                                                         TTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTAATAAATAGCGAAAACCCGCGAGGTCGCAGCCCCGTAAACTGTCTGATCACCGGAAAGGACCCGTAAAGTG             >  NB501016:230:HFVJLAFXY:3:11402:15395:1112/1‑151 (MQ=32)
                                                                                                                                          TTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGTAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAACCCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTG             <  NB501016:230:HFVJLAFXY:2:21111:26793:16739/150‑1 (MQ=34)
                                                                                                                                          TTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTG             <  NB501016:230:HFVJLAFXY:1:21309:6301:14696/150‑1 (MQ=36)
                                                                                                                                            CTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCG                                                 >  NB501016:230:HFVJLAFXY:4:21412:21228:7290/1‑112 (MQ=8)
                                                                                                                                            CTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                   >  NB501016:230:HFVJLAFXY:2:21208:11622:18792/1‑110 (MQ=8)
                                                                                                                                             TTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                            >  NB501016:230:HFVJLAFXY:1:11201:9307:1598/1‑52 (MQ=60)
                                                                                                                                              TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAA         <  NB501016:230:HFVJLAFXY:3:11606:23751:9194/150‑1 (MQ=44)
                                                                                                                                              TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATA          <  NB501016:230:HFVJLAFXY:3:11404:15172:12600/149‑1 (MQ=42)
                                                                                                                                              TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAT           <  NB501016:230:HFVJLAFXY:4:21608:5392:9529/148‑1 (MQ=40)
                                                                                                                                              TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAT           <  NB501016:230:HFVJLAFXY:3:21607:23552:9088/148‑1 (MQ=40)
                                                                                                                                              TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAT           <  NB501016:230:HFVJLAFXY:1:21303:17929:17180/148‑1 (MQ=40)
                                                                                                                                               TTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAAT        <  NB501016:230:HFVJLAFXY:3:21403:13408:9920/150‑1 (MQ=46)
                                                                                                                                               TTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAAT        <  NB501016:230:HFVJLAFXY:4:21403:13159:18961/150‑1 (MQ=46)
                                                                                                                                                TCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAACCGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTC                                                          >  NB501016:230:HFVJLAFXY:3:21406:24019:10356/1‑99 (MQ=8)
                                                                                                                                                 CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT     <  NB501016:230:HFVJLAFXY:4:11509:20882:18731/151‑1 (MQ=52)
                                                                                                                                                 CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT     <  NB501016:230:HFVJLAFXY:4:21509:10408:11031/151‑1 (MQ=52)
                                                                                                                                                 CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGA      <  NB501016:230:HFVJLAFXY:4:11508:15225:16973/150‑1 (MQ=50)
                                                                                                                                                 CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTC                                                                                   >  NB501016:230:HFVJLAFXY:3:21602:7244:10058/1‑73 (MQ=10)
                                                                                                                                                  AGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATT    >  NB501016:230:HFVJLAFXY:1:21108:21532:11018/1‑151 (MQ=54)
                                                                                                                                                  AGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAG                                                                           >  NB501016:230:HFVJLAFXY:1:21102:20951:6564/1‑80 (MQ=9)
                                                                                                                                                   GAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTA   <  NB501016:230:HFVJLAFXY:1:11101:24388:17623/151‑1 (MQ=57)
                                                                                                                                                   GAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTA   <  NB501016:230:HFVJLAFXY:3:21611:19573:4606/151‑1 (MQ=57)
                                                                                                                                                    AGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTAT  <  NB501016:230:HFVJLAFXY:1:11108:10370:16593/151‑1 (MQ=59)
                                                                                                                                                    |                                                                                                                                                      
TCTGAATGGTACGGATACTCGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTAT  >  NC_000913/1636307‑1636605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑