Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F35 I0 R1 338 60.8 3106128 78.2% 2428992 142.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,636,484 C→T 93.0% intergenic (‑117/‑272) nohQ ← / → ynfO pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,636,4840CT93.0% 1499.4 / 62.2 675intergenic (‑117/‑272)nohQ/ynfOpseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base T (300/325);  total (325/350)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 679 total aligned reads displayed.

TCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG  >  NC_000913/1636339‑1636630
                                                                                                                                                 |                                                                                                                                                  
     cagcTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGATTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACgaaga                                                                                                                                            <  8:313983/149‑1 (MQ=11)
           ctatttGTTCACTTCCATTGTTCATTCCACCGACAAAACCAGAGAAACGAAACGACAGAGGCCAACAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACgaagaagaa                                                                                                                                        <  6:363682/144‑1 (MQ=1)
                     aCTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGG‑CCAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCtt                                                                                                                            <  2:62553/148‑1 (MQ=11)
                        tCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCtt                                                                                                                            <  2:335296/146‑1 (MQ=255)
                               ttCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGaa                                                                                                                   >  8:59345/1‑148 (MQ=255)
                                         ggCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATa                                                                                                         >  1:192156/1‑148 (MQ=255)
                                                                               gCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGt                                                                  >  3:201465/1‑149 (MQ=39)
                                                                                   gctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCtt                                                                                                                            >  3:194353/3‑87 (MQ=11)
                                                                                   gctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCtt                                                                                                                            >  3:119251/3‑87 (MQ=11)
                                                                                   gctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCg                                                                <  3:348760/145‑1 (MQ=2)
                                                                                   gctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGa                                                              >  3:279718/3‑149 (MQ=2)
                                                                                   gctttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAACAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGACGTCGCAGCCCCGTAACCTGTCgg                                                               >  2:141268/3‑148 (MQ=11)
                                                                                     tttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAATTTATGACGAAGAAGAACGGAAACGCCTAAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGAt                                                             >  4:249545/1‑148 (MQ=11)
                                                                                     tttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGAt                                                             >  5:282719/1‑148 (MQ=12)
                                                                                     tttCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCAGCCCCGTAACCTGTCGGATc                                                            >  6:85214/1‑149 (MQ=255)
                                                                                     tttAAGCAAATGTCGTTTCCTTTCTTTTAACAGATTATTTTAAATAAAAACATTAAATTAAGACCAACAACAACTGAAACGCCTTAAAACCAAAAAATTTCATAAATAAATAAAACCCGCGAGCACGCCGCACCGAACCCTGACGGAt                                                             >  4:172490/1‑148 (MQ=255)
                                                                                       tCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATaaa                                                                                                       >  7:64137/1‑104 (MQ=11)
                                                                                       tCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                             <  2:387647/130‑1 (MQ=12)
                                                                                       tCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                             >  1:387647/1‑130 (MQ=12)
                                                                                       tCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                             >  8:375825/1‑130 (MQ=12)
                                                                                         aGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCTGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGGAACCGGTCGGATCACCg                                                        >  5:363787/1‑149 (MQ=11)
                                                                                         aGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTcgccg                                                                               >  2:168120/1‑126 (MQ=12)
                                                                                           cACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCgg                                                       >  7:390000/1‑148 (MQ=255)
                                                                                             ccTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCtt                                                                                                                            >  5:118161/1‑77 (MQ=11)
                                                                                             ccTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATATCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGaaa                                                    >  6:178958/1‑149 (MQ=255)
                                                                                             ccTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGaaa                                                    <  2:210561/149‑1 (MQ=255)
                                                                                             ccTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGaaa                                                    <  1:224143/149‑1 (MQ=255)
                                                                                             ccTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGaaa                                                    <  7:192956/149‑1 (MQ=255)
                                                                                              cTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGcc                                                                                                                              <  5:319640/74‑1 (MQ=11)
                                                                                              cTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCg                                                                                                                      <  7:96576/82‑1 (MQ=11)
                                                                                              cTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAAAATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGTAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                             <  8:84117/123‑1 (MQ=11)
                                                                                               tGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                                                   >  7:133214/1‑148 (MQ=255)
                                                                                                gTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTcg                                                                                  <  4:12344/116‑1 (MQ=255)
                                                                                                gTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGCAAGGa                                                 >  7:263197/1‑149 (MQ=255)
                                                                                                 tCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTc                                                                                                            <  4:19619/89‑1 (MQ=11)
                                                                                                 tCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGa                                                 <  1:58255/148‑1 (MQ=255)
                                                                                                 tCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAc                                                <  4:44628/149‑1 (MQ=255)
                                                                                                 cgctctCCTTTCTTTTCAGGGGGTATTTTAACTAAAAACATTAAGTTACGACGAAGACGACCGGAAATGCCTTAAACCGGAAACTTTTCATAAATCGCGAAACCCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGa                                                 <  1:291714/143‑1 (MQ=14)
                                                                                                   gTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAAtttt                                                                                                             >  5:221306/1‑86 (MQ=255)
                                                                                                   gTTTCCTTTATTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAAtttt                                                                                                             <  6:221306/86‑1 (MQ=11)
                                                                                                    tttCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAACCGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCg                                             >  2:77831/1‑149 (MQ=255)
                                                                                                       ccTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAcc                                                                                                                       <  3:215852/72‑1 (MQ=255)
                                                                                                        ctttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTaaa                                         <  5:201386/149‑1 (MQ=255)
                                                                                                        ctttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTaaa                                         <  3:309673/149‑1 (MQ=255)
                                                                                                         tttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCTCCGCCCCGTAACCTGTCGGATCACCGGAAAGGa                                                 >  1:178432/1‑140 (MQ=255)
                                                                                                         tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATa                                                                                                         >  6:272181/1‑84 (MQ=11)
                                                                                                         tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCg                                                        <  6:380572/133‑1 (MQ=255)
                                                                                                         tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                                                   >  8:185992/1‑138 (MQ=255)
                                                                                                         tttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg                                        >  7:302312/1‑149 (MQ=255)
                                                                                                          ttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                       >  1:79073/1‑149 (MQ=255)
                                                                                                          ttctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTAATAAATAGCGAAAACCCGCGAGGTCGCAGCCCCGTAAACTGTCTGATCACCGGAAAGGACCCGTAAAGt                                       >  5:5334/1‑149 (MQ=14)
                                                                                                           tctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                       <  6:204851/148‑1 (MQ=255)
                                                                                                           tctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAACCCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                       <  3:240519/148‑1 (MQ=255)
                                                                                                            ctttTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCgccgcc                                                                             >  2:357403/1‑109 (MQ=255)
                                                                                                               ttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGa                                     <  1:347806/146‑1 (MQ=255)
                                                                                                               ttCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATa                                   <  1:157379/148‑1 (MQ=255)
                                                                                                                tCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATaa                                  <  1:125708/148‑1 (MQ=255)
                                                                                                                 cAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAAt                                 <  7:221807/148‑1 (MQ=255)
                                                                                                                  agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                                              <  8:336781/131‑1 (MQ=255)
                                                                                                                  agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAccc                                              >  7:336781/4‑134 (MQ=255)
                                                                                                                  agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGa                               >  2:258890/4‑149 (MQ=255)
                                                                                                                  agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGa                               <  8:270914/146‑1 (MQ=255)
                                                                                                                  agagggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCAGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGa                               >  8:353972/4‑149 (MQ=255)
                                                                                                                    agtgTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAtt                             <  4:109813/146‑1 (MQ=255)
                                                                                                                     gggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGCCCCGTAAAGTGATAATGATTa                            >  4:271102/1‑149 (MQ=255)
                                                                                                                     gggTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTa                            <  6:342324/149‑1 (MQ=255)
                                                                                                                       ggaTTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAACCCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGTAAAGGACCCGTAAAGTGATAATGATTatc                          <  2:113803/147‑1 (MQ=255)
                                                                                                                       gTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTatc                          <  6:129304/149‑1 (MQ=255)
                                                                                                                          ttttAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAGAATGATTatcctc                       >  6:46265/1‑149 (MQ=255)
                                                                                                                            ttAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCt                      <  2:186697/148‑1 (MQ=255)
                                                                                                                              aaataaaAACATTAAGTTATGACGAAGAAGAACGGAAAGGCCTTAATCCGGAAAATTTTCATTATTAGCTAACACCCGAGAGGTCGCCGCCCCGTAACCGGTCGGATCACCGGAAAGGACGCGTAAAGTGATAATGATTATCATCTAc                    >  6:300442/1‑148 (MQ=14)
                                                                                                                              aaataaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCg                                             >  2:89394/1‑123 (MQ=255)
                                                                                                                              aaataaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACa                   >  1:98463/1‑149 (MQ=255)
                                                                                                                              aaataaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACATGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAc                    <  3:70332/148‑1 (MQ=255)
                                                                                                                              aaataaaAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACa                   >  7:366207/1‑149 (MQ=255)
                                                                                                                               aagaaaaaCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACa                   <  4:217836/145‑1 (MQ=255)
                                                                                                                                ataaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGaaa                                                    <  8:210617/114‑1 (MQ=255)
                                                                                                                                ataaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTaaa                                         >  5:186018/1‑125 (MQ=255)
                                                                                                                                ataaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACata                 >  8:125979/1‑149 (MQ=255)
                                                                                                                                 taaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACatat                >  5:344189/1‑149 (MQ=255)
                                                                                                                                 taaaAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACata                 >  5:257360/1‑148 (MQ=255)
                                                                                                                                  aaaaaCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGAt                                                             <  4:335058/103‑1 (MQ=12)
                                                                                                                                   aaaaCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATc               <  1:258890/148‑1 (MQ=255)
                                                                                                                                   aaaaCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCAAAAATAGCGAAAACCCCCGAGGTCGCCGCCCCGTAACCTGGCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCCTCTACGAATca              >  6:32062/1‑149 (MQ=14)
                                                                                                                                    aaaCATTAAGTTATGACGAAGAATAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATcac             <  5:8963/149‑1 (MQ=255)
                                                                                                                                     aaCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCcg                                                                                          >  7:106394/1‑71 (MQ=9)
                                                                                                                                     aaCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATcaca            <  1:370018/149‑1 (MQ=255)
                                                                                                                                      aCATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCGCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACaa           <  6:152296/149‑1 (MQ=255)
                                                                                                                                       cATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAc          >  5:158817/1‑149 (MQ=255)
                                                                                                                                       cATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAc          >  7:51002/1‑149 (MQ=255)
                                                                                                                                         ttAAGTTATGACGAAGAAGAACGGAAACGCCTTATACCTGAAAATTTTCATAAATAGCTAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgt        <  6:248898/149‑1 (MQ=14)
                                                                                                                                         ttAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgt        >  3:16228/1‑149 (MQ=255)
                                                                                                                                            aGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgc      <  1:3218/148‑1 (MQ=255)
                                                                                                                                             gTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgt    <  8:234740/149‑1 (MQ=255)
                                                                                                                                             gTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgt    <  5:246699/149‑1 (MQ=255)
                                                                                                                                             gTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgt    <  4:210133/149‑1 (MQ=255)
                                                                                                                                             gTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgt    <  1:100139/149‑1 (MQ=255)
                                                                                                                                              ttATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgtg   >  5:381027/1‑149 (MQ=255)
                                                                                                                                              ttATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGGCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgtgcgtg   <  2:304515/149‑1 (MQ=255)
                                                                                                                                                acgacgAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTgg  <  4:308352/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG  >  NC_000913/1636339‑1636630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 584 total aligned reads displayed.
AAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG  >  NC_000913/1636334‑1636633
                                                                                                                                                      |                                                                                                                                                     
AAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTAT                                                                                                                                                       <  NB501016:230:HFVJLAFXY:1:11101:14060:4846/151‑1 (MQ=0)
AAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTAT                                                                                                                                                       <  NB501016:230:HFVJLAFXY:4:11406:1052:10663/151‑1 (MQ=0)
 AATGTCAGCCAGCTGCTTTTTTTTTACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACTAAAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTAGTTTCCTTTCTTTTCATAGTGTATTTTAAATAAAAACATTAAGTTATG                                                                                                                                                      <  NB501016:230:HFVJLAFXY:2:11306:23366:1677/151‑1 (MQ=0)
 AATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATG                                                                                                                                                      <  NB501016:230:HFVJLAFXY:1:11309:23750:11341/151‑1 (MQ=0)
 AATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTAATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGACAAAAAGCTCGCTTTCAGAACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATG                                                                                                                                                      >  NB501016:230:HFVJLAFXY:3:11506:1805:2688/1‑151 (MQ=0)
 AATGTCAGCAAGCTGCTTTTTGATGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATG                                                                                                                                                      <  NB501016:230:HFVJLAFXY:1:11106:5010:6691/151‑1 (MQ=0)
  ATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGA                                                                                                                                                     <  NB501016:230:HFVJLAFXY:4:11504:16175:5332/151‑1 (MQ=0)
  ATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGA                                                                                                                                                     <  NB501016:230:HFVJLAFXY:4:11601:19121:9689/151‑1 (MQ=0)
   TGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTACCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAACTTATGAC                                                                                                                                                    >  NB501016:230:HFVJLAFXY:2:11109:1274:18303/1‑151 (MQ=0)
    GTCAGCCTGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGAC                                                                                                                                                    <  NB501016:230:HFVJLAFXY:1:11208:7913:17428/150‑1 (MQ=0)
    GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACG                                                                                                                                                   >  NB501016:230:HFVJLAFXY:1:21306:14890:17251/1‑151 (MQ=60)
    GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCTCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGAC                                                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11609:10107:1857/150‑1 (MQ=0)
    GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACG                                                                                                                                                   <  NB501016:230:HFVJLAFXY:2:21101:18028:10620/151‑1 (MQ=0)
    GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACG                                                                                                                                                   <  NB501016:230:HFVJLAFXY:2:21307:18419:20044/151‑1 (MQ=0)
    GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGAC                                                                                                                                                    <  NB501016:230:HFVJLAFXY:3:11505:17400:13850/150‑1 (MQ=0)
    GTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCACTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGAC                                                                                                                                                    <  NB501016:230:HFVJLAFXY:4:21405:9833:16307/150‑1 (MQ=0)
      CAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAA                                                                                                                                                 <  NB501016:230:HFVJLAFXY:4:11402:21458:10677/151‑1 (MQ=0)
      CAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                                                  >  NB501016:230:HFVJLAFXY:4:11405:13408:14778/1‑150 (MQ=0)
      CAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTACTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                                                  >  NB501016:230:HFVJLAFXY:2:11201:15996:1733/1‑150 (MQ=0)
       AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACATAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                                                <  NB501016:230:HFVJLAFXY:1:21104:18431:15931/151‑1 (MQ=0)
       AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAATAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                                                <  NB501016:230:HFVJLAFXY:2:21207:10770:4121/151‑1 (MQ=0)
       AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAT                                                                                                                                                >  NB501016:230:HFVJLAFXY:4:11502:14331:8334/1‑151 (MQ=0)
       AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                                                <  NB501016:230:HFVJLAFXY:1:21110:23845:5388/151‑1 (MQ=0)
       AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                                                <  NB501016:230:HFVJLAFXY:3:21409:1329:14802/151‑1 (MQ=0)
       AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                                                <  NB501016:230:HFVJLAFXY:3:21507:2600:6844/151‑1 (MQ=0)
       AGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                                                <  NB501016:230:HFVJLAFXY:2:11302:17641:7645/151‑1 (MQ=0)
       AGCCAGCTGATTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                                                <  NB501016:230:HFVJLAFXY:2:21304:15009:16266/151‑1 (MQ=0)
        GCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGA                                                                                                                                               <  NB501016:230:HFVJLAFXY:4:11511:24561:8717/151‑1 (MQ=0)
        GCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                                                  >  NB501016:230:HFVJLAFXY:1:21112:15163:8091/1‑148 (MQ=0)
        GCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGA                                                                                                                                                  >  NB501016:230:HFVJLAFXY:2:11102:20183:4272/1‑148 (MQ=0)
         CCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGA                                                                                                                                               >  NB501016:230:HFVJLAFXY:4:21409:3784:5803/1‑150 (MQ=0)
         CCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGA                                                                                                                                               >  NB501016:230:HFVJLAFXY:3:11512:10946:8718/1‑150 (MQ=0)
         CCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGAAGAAGA                                                                                                                                               >  NB501016:230:HFVJLAFXY:2:21107:4934:14469/1‑150 (MQ=0)
          CAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAG                                                                                                                                             <  NB501016:230:HFVJLAFXY:2:11205:7647:10400/151‑1 (MQ=0)
          CAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAG                                                                                                                                             <  NB501016:230:HFVJLAFXY:3:11406:4380:19331/151‑1 (MQ=0)
           AGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGA                                                                                                                                            <  NB501016:230:HFVJLAFXY:1:11103:21933:14457/151‑1 (MQ=0)
           AGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGA                                                                                                                                            <  NB501016:230:HFVJLAFXY:3:11607:2930:17498/151‑1 (MQ=0)
           AGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAG                                                                                                                                                >  NB501016:230:HFVJLAFXY:2:21308:9171:8714/1‑147 (MQ=0)
            GCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGA                                                                                                                                            >  NB501016:230:HFVJLAFXY:2:21103:5988:12352/1‑150 (MQ=0)
              TGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                                                         >  NB501016:230:HFVJLAFXY:4:21605:9220:2788/1‑151 (MQ=0)
              TGCTTTTTGTGGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTAATTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                                                         >  NB501016:230:HFVJLAFXY:2:21112:23231:6733/1‑151 (MQ=0)
               ggacTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:4:21511:14308:11101/147‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTTTTCTTTCCACGGACTAATACAGAGATAGGAAACGACAGTGGCCAAAAAGCTCTATTTGATCAGCTGTTGTTTCCTTTCTTTTCAGAGGGTATTTTATATTATAACATTAAGTTATGACGAAGAAGttcgt                                                                                                                                        <  NB501016:230:HFVJLAFXY:1:11303:10533:1445/151‑6 (MQ=3)
               GCTTTTTGTTGACTTCCATTTTTCATTCCACTGACAAAAACAGAGAAAGTAAACTACATAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                                                          <  NB501016:230:HFVJLAFXY:4:21607:18206:2984/149‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGATAAAGGAAACGACAGAGGCCATAAAGCTCGCTTTAAGAACCTTTCTTTTACTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                                                          <  NB501016:230:HFVJLAFXY:4:21501:2503:6641/149‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGTAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:3:21509:16167:11290/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:1:21207:1807:8066/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:3:21405:19175:13136/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:2:21105:5827:4739/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:1:11204:21556:5762/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:1:21102:25739:10405/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:2:21304:21432:7792/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:4:21507:5280:17653/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                                                         <  NB501016:230:HFVJLAFXY:4:21506:16078:9701/150‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                                                         <  NB501016:230:HFVJLAFXY:1:11309:13188:18826/150‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                                                         <  NB501016:230:HFVJLAFXY:2:11301:9752:13434/150‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                                                         <  NB501016:230:HFVJLAFXY:4:21504:9636:16527/150‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                                                          <  NB501016:230:HFVJLAFXY:2:11211:25214:17042/149‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                                                          <  NB501016:230:HFVJLAFXY:2:21208:6798:4924/149‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                                                          <  NB501016:230:HFVJLAFXY:3:21405:6440:7690/149‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                                                          <  NB501016:230:HFVJLAFXY:4:21608:16719:11170/149‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                                                          <  NB501016:230:HFVJLAFXY:2:11203:3984:15445/149‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTACTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:3:11407:7815:12405/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAAAGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        <  NB501016:230:HFVJLAFXY:4:21611:25778:2647/151‑1 (MQ=0)
               GCTTTTTGTTGACTTCCATATTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTAGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGTGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                                                         <  NB501016:230:HFVJLAFXY:2:21106:7813:1512/150‑1 (MQ=0)
                CTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:4:11603:21287:16939/1‑150 (MQ=0)
                CTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:3:21411:2465:17003/1‑150 (MQ=0)
                 TTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTCTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                                                          >  NB501016:230:HFVJLAFXY:4:21405:11369:17054/1‑147 (MQ=0)
                     TGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                                                         >  NB501016:230:HFVJLAFXY:3:21610:8597:20176/1‑144 (MQ=0)
                        TGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAC                                                                                                                                          <  NB501016:230:HFVJLAFXY:4:21503:2570:5932/140‑1 (MQ=0)
                         GACTTCCATTGTTCATTCCACGGACAAAAACAGTGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                                              <  NB501016:230:HFVJLAFXY:3:11509:15304:6356/151‑1 (MQ=0)
                         GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAATCGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTT                                                                                                                               <  NB501016:230:HFVJLAFXY:4:11605:7045:7674/150‑1 (MQ=0)
                         GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCTCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                                              <  NB501016:230:HFVJLAFXY:3:21603:20917:18861/151‑1 (MQ=0)
                         GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTA                                                                                                                              <  NB501016:230:HFVJLAFXY:4:11608:9105:8864/151‑1 (MQ=0)
                         GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTT                                                                                                                               <  NB501016:230:HFVJLAFXY:2:21110:8572:14899/150‑1 (MQ=0)
                         GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTT                                                                                                                               <  NB501016:230:HFVJLAFXY:1:11104:8447:4424/150‑1 (MQ=0)
                         GACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGATATGACGAAGAAGAACGGAAACGCCTT                                                                                                                               >  NB501016:230:HFVJLAFXY:3:21605:26425:18886/1‑150 (MQ=0)
                          ACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTTGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAA                                                                                                                             <  NB501016:230:HFVJLAFXY:4:11602:15535:3547/151‑1 (MQ=0)
                           CTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTGTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGACCGGAAACGCCTTAA                                                                                                                             >  NB501016:230:HFVJLAFXY:1:11209:3661:2194/1‑150 (MQ=0)
                                              cgaacaaaacacactaaagaaaagacacacgccaaaaaccccgcctttaaccacatgagttttcatttctttaatacgggaattttAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAAT                                                                                                         <  NB501016:230:HFVJLAFXY:3:11411:19460:7890/65‑1 (MQ=11)
                                                                  atacgagatcatagccggtgactggagttcagacgtgtgctccttccgatctcagaGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCG                                                                                     <  NB501016:230:HFVJLAFXY:2:21203:18338:9036/95‑1 (MQ=9)
                                                                   actacagatgacaaaaagatctcTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGTGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGC                                                                                    <  NB501016:230:HFVJLAFXY:3:11407:17309:8504/128‑1 (MQ=3)
                                                                               CAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACC                                                                        <  NB501016:230:HFVJLAFXY:4:11410:20085:18860/151‑1 (MQ=45)
                                                                               AAAAAGCCCGCTTTCAGCACATGTCGTTTCCTTTCTTTTCATAGTGTATTTAAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAAAGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACC                                                                        <  NB501016:230:HFVJLAFXY:1:21107:1111:17550/151‑1 (MQ=4)
                                                                                AAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCAGCGAGGTCGCCGCCCCGTAAGCT                                                                       >  NB501016:230:HFVJLAFXY:3:11503:12326:16760/1‑151 (MQ=0)
                                                                                  AAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGATACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                               >  NB501016:230:HFVJLAFXY:2:11209:11519:3928/1‑141 (MQ=0)
                                                                                  AAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGT                                                                     <  NB501016:230:HFVJLAFXY:1:11311:6464:17909/151‑1 (MQ=0)
                                                                                     CTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGagatcggaaagagcacacgtctgaact                                                                  >  NB501016:230:HFVJLAFXY:3:11603:5344:15361/1‑124 (MQ=0)
                                                                                       CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGG                                                                  <  NB501016:230:HFVJLAFXY:3:11606:25501:15209/149‑1 (MQ=1)
                                                                                         CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATC                                                               >  NB501016:230:HFVJLAFXY:4:21611:12590:18080/1‑150 (MQ=5)
                                                                                         CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATC                                                               >  NB501016:230:HFVJLAFXY:1:11306:3668:18077/1‑150 (MQ=5)
                                                                                         CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATC                                                               >  NB501016:230:HFVJLAFXY:4:11402:1463:10841/1‑150 (MQ=5)
                                                                                         CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGAGAGGTCG                                                                                     >  NB501016:230:HFVJLAFXY:4:11411:9049:1480/1‑128 (MQ=5)
                                                                                         CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAAACCGCGAGGTCGCCTCCCAGTAACCTGTCGGATC                                                               >  NB501016:230:HFVJLAFXY:2:11105:10581:2355/1‑150 (MQ=4)
                                                                                           TTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                               >  NB501016:230:HFVJLAFXY:1:21311:16465:5035/1‑132 (MQ=7)
                                                                                             CAGCTCCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGTAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGG                                                          <  NB501016:230:HFVJLAFXY:1:21202:20903:8663/151‑1 (MQ=19)
                                                                                             CAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCTGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGGAACCGGTCGGATCACCGG                                                          >  NB501016:230:HFVJLAFXY:3:21609:26734:6443/1‑151 (MQ=18)
                                                                                             CAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                               >  NB501016:230:HFVJLAFXY:3:21611:22975:18972/1‑130 (MQ=7)
                                                                                              AGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCC                                                                                <  NB501016:230:HFVJLAFXY:1:11106:21498:13226/128‑1 (MQ=7)
                                                                                                CACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAA                                                        >  NB501016:230:HFVJLAFXY:1:11204:17127:6861/1‑150 (MQ=20)
                                                                                                 ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                      <  NB501016:230:HFVJLAFXY:4:11610:16876:14121/151‑1 (MQ=20)
                                                                                                 ACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                      <  NB501016:230:HFVJLAFXY:1:11110:19437:2044/151‑1 (MQ=20)
                                                                                                  CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATATCGAAAACCCGCGCGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                                                     >  NB501016:230:HFVJLAFXY:1:11308:21985:19921/1‑151 (MQ=19)
                                                                                                  CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTAATAAATAGCGAAAAACAGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGG                                                     >  NB501016:230:HFVJLAFXY:3:11411:9372:3180/1‑151 (MQ=18)
                                                                                                  CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGG                                                                                                                        <  NB501016:230:HFVJLAFXY:4:11505:25813:6974/84‑1 (MQ=9)
                                                                                                  CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCT                                                                                                                                <  NB501016:230:HFVJLAFXY:3:21601:3376:11865/76‑1 (MQ=7)
                                                                                                  CCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAAAGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                               >  NB501016:230:HFVJLAFXY:4:11503:17499:1708/1‑125 (MQ=7)
                                                                                                   CTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                      <  NB501016:230:HFVJLAFXY:3:21505:4664:7568/149‑1 (MQ=43)
                                                                                                   CTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCG                                                           >  NB501016:230:HFVJLAFXY:4:21609:18478:10557/1‑144 (MQ=18)
                                                                                                    TGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCagatcggaagagcacacgtctggaactccagtc                                                   >  NB501016:230:HFVJLAFXY:2:11103:9836:9392/1‑118 (MQ=8)
                                                                                                    TGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGCAAGGAC                                                   >  NB501016:230:HFVJLAFXY:4:21411:25794:4782/1‑151 (MQ=19)
                                                                                                     ccgctcTCCTTTCTTTTCAGGGGGTATTTTAACTAAAAACATTAAGTTACGACGAAGACGACCGGAAATGCCTTAAACCGGAAACTTTTCATAAATCGCGAAACCCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                                                   <  NB501016:230:HFVJLAFXY:1:21205:1566:2891/144‑1 (MQ=37)
                                                                                                     GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAC                                                   <  NB501016:230:HFVJLAFXY:2:11309:5630:18224/150‑1 (MQ=20)
                                                                                                     GTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAAT                                                                                                         <  NB501016:230:HFVJLAFXY:2:11107:2554:6573/96‑1 (MQ=8)
                                                                                                       CGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGCACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCGGCGAGGTGGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCG                                                <  NB501016:230:HFVJLAFXY:1:11304:6998:13283/151‑1 (MQ=20)
                                                                                                       CGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCG                                                >  NB501016:230:HFVJLAFXY:3:21601:17393:11832/1‑151 (MQ=22)
                                                                                                         TTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGCCGAAGAAGAACGGAACCGCCTTAAACCGGAAAATTTTCATATATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGT                                               >  NB501016:230:HFVJLAFXY:1:11104:23916:18466/1‑150 (MQ=22)
                                                                                                          TTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAATAACGGAAACGCCTTAAAACGTAAAATTTTCATAAATAGCGAAAACCCTCGAGGTCGCCGCCCCGTAACCTGTCGTATCACCGGAAAGGACCCGTA                                              >  NB501016:230:HFVJLAFXY:4:21605:10786:8507/1‑150 (MQ=23)
                                                                                                          TTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAAACGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTA                                              >  NB501016:230:HFVJLAFXY:2:11312:11293:19109/1‑150 (MQ=25)
                                                                                                           TCCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACG                                                                                                                                         >  NB501016:230:HFVJLAFXY:4:11509:6163:6871/1‑58 (MQ=60)
                                                                                                           TCCTTTCTTTTCAGAGGGTAATTTAAATAAAAACATTAAGTTATGACGAAGAAGAACCGAACAGCCTTAAACCGGAAAATTTTCATAAATAGAGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAA                                             >  NB501016:230:HFVJLAFXY:1:11106:1844:3234/1‑150 (MQ=34)
                                                                                                            CCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAATTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAG                                           <  NB501016:230:HFVJLAFXY:2:21201:1819:14564/151‑1 (MQ=31)
                                                                                                            CCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAG                                           <  NB501016:230:HFVJLAFXY:2:21301:25669:17328/151‑1 (MQ=32)
                                                                                                            CCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAG                                           <  NB501016:230:HFVJLAFXY:3:21402:3591:5574/151‑1 (MQ=32)
                                                                                                            CCTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCA                                                                                                              <  NB501016:230:HFVJLAFXY:3:21411:13166:2574/84‑1 (MQ=9)
                                                                                                             CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                          >  NB501016:230:HFVJLAFXY:2:11201:8906:10821/1‑151 (MQ=34)
                                                                                                             CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                          >  NB501016:230:HFVJLAFXY:1:11310:19805:2697/1‑151 (MQ=34)
                                                                                                             CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                          >  NB501016:230:HFVJLAFXY:4:11509:21550:7523/1‑151 (MQ=34)
                                                                                                             CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                          >  NB501016:230:HFVJLAFXY:1:11310:9249:13269/1‑151 (MQ=34)
                                                                                                             CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGT                                          >  NB501016:230:HFVJLAFXY:2:21101:8371:10568/1‑151 (MQ=34)
                                                                                                             CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTC                                                                    <  NB501016:230:HFVJLAFXY:3:11612:18667:20312/125‑1 (MQ=8)
                                                                                                             CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAG                                                                                         >  NB501016:230:HFVJLAFXY:1:21201:14382:11604/1‑104 (MQ=8)
                                                                                                             CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCG                                                                                           >  NB501016:230:HFVJLAFXY:3:11405:12701:6343/1‑102 (MQ=8)
                                                                                                             CTTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAA                                                                                                           <  NB501016:230:HFVJLAFXY:3:21504:10364:13275/86‑1 (MQ=9)
                                                                                                              TTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTG                                         >  NB501016:230:HFVJLAFXY:1:11202:8470:8630/1‑151 (MQ=36)
                                                                                                              TTTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTAATAAATAGCGAAAACCCGCGAGGTCGCAGCCCCGTAAACTGTCTGATCACCGGAAAGGACCCGTAAAGTG                                         >  NB501016:230:HFVJLAFXY:3:11402:15395:1112/1‑151 (MQ=32)
                                                                                                               TTCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGG                                                                                                                        >  NB501016:230:HFVJLAFXY:3:11511:3912:20146/1‑71 (MQ=10)
                                                                                                                TCTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                               <  NB501016:230:HFVJLAFXY:1:21305:5525:12455/111‑1 (MQ=8)
                                                                                                                  TTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGG                                                                                                                                        >  NB501016:230:HFVJLAFXY:1:11201:9307:1598/1‑52 (MQ=60)
                                                                                                                   TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAA                                     <  NB501016:230:HFVJLAFXY:1:11305:16619:6261/150‑1 (MQ=44)
                                                                                                                   TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATA                                      <  NB501016:230:HFVJLAFXY:3:11404:15172:12600/149‑1 (MQ=42)
                                                                                                                   TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATA                                      <  NB501016:230:HFVJLAFXY:3:11409:8245:6931/149‑1 (MQ=42)
                                                                                                                   TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAT                                       <  NB501016:230:HFVJLAFXY:3:21607:23552:9088/148‑1 (MQ=40)
                                                                                                                   TTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAT                                       <  NB501016:230:HFVJLAFXY:1:21303:17929:17180/148‑1 (MQ=40)
                                                                                                                    TTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAATCCGGAAAATTTTCATAAATAGCGAATACCCGAGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAAT                                    <  NB501016:230:HFVJLAFXY:2:21103:16762:13667/150‑1 (MQ=42)
                                                                                                                    TTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAAT                                    <  NB501016:230:HFVJLAFXY:4:21403:13159:18961/150‑1 (MQ=46)
                                                                                                                    TTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAAT                                    <  NB501016:230:HFVJLAFXY:3:11610:24343:9927/150‑1 (MQ=46)
                                                                                                                      CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT                                 <  NB501016:230:HFVJLAFXY:2:21104:18859:11484/151‑1 (MQ=52)
                                                                                                                      CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT                                 <  NB501016:230:HFVJLAFXY:4:21509:10408:11031/151‑1 (MQ=52)
                                                                                                                      CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAT                                 <  NB501016:230:HFVJLAFXY:4:21408:11509:8560/151‑1 (MQ=52)
                                                                                                                      CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCG                                                >  NB501016:230:HFVJLAFXY:4:21601:8933:7229/1‑136 (MQ=23)
                                                                                                                      CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                               <  NB501016:230:HFVJLAFXY:1:21309:24049:17419/105‑1 (MQ=8)
                                                                                                                      CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCC                                                                               <  NB501016:230:HFVJLAFXY:1:11111:21770:18051/105‑1 (MQ=8)
                                                                                                                      CAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGC                                                                                                                                  <  NB501016:230:HFVJLAFXY:3:11511:15798:6669/54‑1 (MQ=8)
                                                                                                                         AGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTAT                              >  NB501016:230:HFVJLAFXY:4:11403:4463:10823/1‑151 (MQ=59)
                                                                                                                         AGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTAT                              <  NB501016:230:HFVJLAFXY:1:11108:10370:16593/151‑1 (MQ=59)
                                                                                                                          GGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGACCGGAAACGACTTAACACGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGACGCCCCGTAACCTGGCGGATCACCGGAAAGGACCCGTACAGTGCTAAGGATTATC                             >  NB501016:230:HFVJLAFXY:3:21501:10000:9164/1‑151 (MQ=24)
                                                                                                                          GGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGTAAACGCCTTAAACCGGATAATTTTCATAAATAGCGAAATCCCGCGAGGTCGCCGCCCagatcggaagagcacacgtctgatctccaagacaccggctatgatctcgt                             >  NB501016:230:HFVJLAFXY:2:11212:24785:1582/1‑101 (MQ=7)
                                                                                                                          GGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACC                                                            >  NB501016:230:HFVJLAFXY:4:11411:12163:10623/1‑120 (MQ=18)
                                                                                                                          GGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGG                                                                  <  NB501016:230:HFVJLAFXY:2:11304:12029:15763/114‑1 (MQ=8)
                                                                                                                           GGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCA                            <  NB501016:230:HFVJLAFXY:1:21301:2869:7104/151‑1 (MQ=60)
                                                                                                                           GGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCA                            <  NB501016:230:HFVJLAFXY:1:21101:16537:12920/151‑1 (MQ=60)
                                                                                                                              ATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCT                         >  NB501016:230:HFVJLAFXY:3:11408:21317:14505/1‑151 (MQ=60)
                                                                                                                              ATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATC                          <  NB501016:230:HFVJLAFXY:2:11306:1281:15222/150‑1 (MQ=60)
                                                                                                                              ATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCAT                           <  NB501016:230:HFVJLAFXY:4:21603:8501:8500/149‑1 (MQ=60)
                                                                                                                               TTTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCT                         >  NB501016:230:HFVJLAFXY:1:11212:21731:16249/1‑150 (MQ=60)
                                                                                                                                TTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTA                        <  NB501016:230:HFVJLAFXY:1:21210:6143:6943/150‑1 (MQ=60)
                                                                                                                                TTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTA                        <  NB501016:230:HFVJLAFXY:2:21207:7149:19365/150‑1 (MQ=60)
                                                                                                                                TTTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTA                        <  NB501016:230:HFVJLAFXY:1:11102:2201:15675/150‑1 (MQ=60)
                                                                                                                                 TTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAC                       <  NB501016:230:HFVJLAFXY:1:11101:15980:4289/150‑1 (MQ=60)
                                                                                                                                 TTAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGC                                                                                                      <  NB501016:230:HFVJLAFXY:1:21108:11583:2515/71‑1 (MQ=10)
                                                                                                                                 TTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAC                       >  NB501016:230:HFVJLAFXY:3:21602:10254:13981/1‑150 (MQ=60)
                                                                                                                                  TAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACAT                     >  NB501016:230:HFVJLAFXY:1:11206:18000:3458/1‑151 (MQ=60)
                                                                                                                                  TAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACATGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACA                      <  NB501016:230:HFVJLAFXY:2:11203:2283:1396/150‑1 (MQ=60)
                                                                                                                                    AATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAG                                           >  NB501016:230:HFVJLAFXY:3:11611:6289:7032/1‑127 (MQ=35)
                                                                                                                                    AATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAG                                                      >  NB501016:230:HFVJLAFXY:4:21401:5542:19636/1‑116 (MQ=22)
                                                                                                                                     ATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGTAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATC                  >  NB501016:230:HFVJLAFXY:3:21401:9627:11148/1‑151 (MQ=60)
                                                                                                                                     ATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATC                  >  NB501016:230:HFVJLAFXY:4:21511:9016:19884/1‑151 (MQ=60)
                                                                                                                                     ATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATAT                   >  NB501016:230:HFVJLAFXY:3:21412:15300:16678/1‑150 (MQ=60)
                                                                                                                                     ATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATCATTATCActttacgggt                  >  NB501016:230:HFVJLAFXY:4:11408:26035:16850/1‑141 (MQ=51)
                                                                                                                                      TAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGAAAAGGACCCGTAAAGTGATAATGATTA                               <  NB501016:230:HFVJLAFXY:1:11206:7545:8677/137‑1 (MQ=57)
                                                                                                                                      TAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATC                                                               >  NB501016:230:HFVJLAFXY:2:21306:12088:13535/1‑105 (MQ=9)
                                                                                                                                       AAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCA                 <  NB501016:230:HFVJLAFXY:4:21406:2252:7877/150‑1 (MQ=60)
                                                                                                                                         AAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAA              <  NB501016:230:HFVJLAFXY:1:21307:8744:19449/151‑1 (MQ=60)
                                                                                                                                         AAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGC                                                                                            >  NB501016:230:HFVJLAFXY:4:11507:4812:2660/1‑73 (MQ=2)
                                                                                                                                           ACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACG            <  NB501016:230:HFVJLAFXY:2:21111:21834:18320/151‑1 (MQ=60)
                                                                                                                                             ATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTG          <  NB501016:230:HFVJLAFXY:2:21102:20230:15931/151‑1 (MQ=60)
                                                                                                                                              TTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCG                                                                                             <  NB501016:230:HFVJLAFXY:1:21212:26280:9581/67‑1 (MQ=0)
                                                                                                                                                AAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCG        <  NB501016:230:HFVJLAFXY:2:21109:5105:7059/150‑1 (MQ=60)
                                                                                                                                                  GTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG     >  NB501016:230:HFVJLAFXY:3:21612:2822:6896/1‑151 (MQ=60)
                                                                                                                                                  GTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG     <  NB501016:230:HFVJLAFXY:3:21407:11994:4702/151‑1 (MQ=60)
                                                                                                                                                  GTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACAT                     >  NB501016:230:HFVJLAFXY:4:11612:7628:18136/1‑135 (MQ=53)
                                                                                                                                                  GTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGA                                  <  NB501016:230:HFVJLAFXY:4:11506:17472:13059/122‑1 (MQ=44)
                                                                                                                                                  GTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG     <  NB501016:230:HFVJLAFXY:1:11211:8891:17274/151‑1 (MQ=60)
                                                                                                                                                   TTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG     <  NB501016:230:HFVJLAFXY:4:11401:11483:1845/150‑1 (MQ=60)
                                                                                                                                                    TATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGA    <  NB501016:230:HFVJLAFXY:1:21103:21230:6508/150‑1 (MQ=60)
                                                                                                                                                    TATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGG     >  NB501016:230:HFVJLAFXY:3:21410:1330:6928/1‑149 (MQ=60)
                                                                                                                                                     ATGACGAAGAAGAACGGAAACGCCTTACACCGGAAAATTTTCCTACATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGCGCCCCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG  <  NB501016:230:HFVJLAFXY:3:21504:2265:19076/151‑1 (MQ=56)
                                                                                                                                                     ATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTACCCTGTCGGATCACAGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG  >  NB501016:230:HFVJLAFXY:1:21105:8226:15605/1‑151 (MQ=60)
                                                                                                                                                     ATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG  <  NB501016:230:HFVJLAFXY:1:11307:2151:8674/151‑1 (MQ=60)
                                                                                                                                                      |                                                                                                                                                     
AAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTGCGTGGAGG  >  NC_000913/1636334‑1636633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑