Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F36 I1 R1 9 107.0 3793126 97.8% 3709677 139.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTA  >  NC_000913/4182790‑4183085
                                                                                                                                                    |                                                                                                                                                   
GACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGC                                                                                                                                                     >  NB501016:230:HFVJLAFXY:1:11104:10906:18548/1‑149 (MQ=60)
  CAAGAACAACCCGCTGTCTGAGATTACGAACAAACGTAGTATCTCCGCAATCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGATTCGAAGTTCGATACGTACACACGACTCACTACTGTCGCGTATGTACAATCGAAACCCCATAAGGT                                                                                                                                                 >  NB501016:230:HFVJLAFXY:4:11412:1102:3532/1‑151 (MQ=60)
        CAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACCCCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAC                                                                                                                                           >  NB501016:230:HFVJLAFXY:1:11110:18785:15447/1‑151 (MQ=60)
           CCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCGGAAGGTCCGAACATC                                                                                                                                        >  NB501016:230:HFVJLAFXY:1:11207:22709:7037/1‑151 (MQ=60)
             CGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGG                                                                                                                                      <  NB501016:230:HFVJLAFXY:2:11303:25347:13002/151‑1 (MQ=60)
                 GTCTGAGATTCCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGACGTTCGAGACGTACACCCGACTCACTACGTTCGCGTATGTCCAATCTATACCCCTGAAGGTCCGAACATCGGTCT                                                                                                                                   <  NB501016:230:HFVJLAFXY:2:21104:2790:3640/150‑1 (MQ=60)
                 GTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTG                                                                                                                                  >  NB501016:230:HFVJLAFXY:3:21611:8545:4438/1‑151 (MQ=60)
                    TGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATC                                                                                                                               >  NB501016:230:HFVJLAFXY:3:11608:14757:5574/1‑151 (MQ=60)
                       GATTACGCACAAACGTCGTATCTCCGCACTCTGCCCAGGCGGTATGACCCGGGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAAC                                                                                                                            <  NB501016:230:HFVJLAFXY:1:11211:5717:1989/151‑1 (MQ=60)
                          TACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCT                                                                                                                         >  NB501016:230:HFVJLAFXY:2:11302:23284:16241/1‑151 (MQ=60)
                          TACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCT                                                                                                                         >  NB501016:230:HFVJLAFXY:3:11608:10433:12591/1‑151 (MQ=60)
                            CGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCC                                                                                                                                               >  NB501016:230:HFVJLAFXY:1:21110:24877:12666/1‑127 (MQ=60)
                            CGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGCACATCGGTCTGATCAACTCTCT                                                                                                                       >  NB501016:230:HFVJLAFXY:4:21506:14921:11749/1‑151 (MQ=60)
                              CACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGT                                                                                                                     >  NB501016:230:HFVJLAFXY:2:11105:6450:13121/1‑151 (MQ=60)
                               ACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTC                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11201:24165:13424/151‑1 (MQ=60)
                                CAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGagatcggaagagcacaccgt                                                                                                                   >  NB501016:230:HFVJLAFXY:1:11102:2787:15280/1‑131 (MQ=60)
                                            CTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCAC                                                                                                         >  NB501016:230:HFVJLAFXY:2:21304:22545:1446/1‑149 (MQ=60)
                                            CTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCAC                                                                                                         >  NB501016:230:HFVJLAFXY:3:21606:13004:9743/1‑149 (MQ=60)
                                             TCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGC                                                                                                      >  NB501016:230:HFVJLAFXY:4:21508:2320:17450/1‑151 (MQ=60)
                                              CCGCACGCGGCCCAGTCGGTCTGACCCGTGAACGGGCCGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAGCGGTCTGATCAACTCTCTGTCCGTGGACGCACA                                                                                                        <  NB501016:230:HFVJLAFXY:3:21501:8692:14440/148‑1 (MQ=60)
                                               CGCACTCGGCCCAGGCGGTCTGAGCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACT                                                                                                    <  NB501016:230:HFVJLAFXY:4:21605:6923:12414/151‑1 (MQ=60)
                                                   CTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACG                                                                                                >  NB501016:230:HFVJLAFXY:1:21103:16799:13852/1‑151 (MQ=60)
                                                        CCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGG                                                                                                                                      <  NB501016:230:HFVJLAFXY:2:11203:4492:10977/108‑1 (MQ=60)
                                                         CCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACG                                                                                          <  NB501016:230:HFVJLAFXY:3:11503:6111:7992/151‑1 (MQ=60)
                                                         CCAGCCGGGCTGACCCGTGAACGTGCAGCCTGCGAAGTTCGAGACGTACACCCGACTCACTCCGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACG                                                                                          <  NB501016:230:HFVJLAFXY:4:11604:20655:10416/151‑1 (MQ=60)
                                                          CAGGCGGTCTGACCCGTGACCGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGG                                                                                         >  NB501016:230:HFVJLAFXY:3:11510:9590:10403/1‑151 (MQ=60)
                                                               GGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCC                                                                                    >  NB501016:230:HFVJLAFXY:3:21608:11986:9183/1‑151 (MQ=60)
                                                                  GTGACCCGTGAACGTGCAGGCTTGGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATGATCTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCGTCCTTG                                                                                 <  NB501016:230:HFVJLAFXY:2:21305:13407:15588/151‑1 (MQ=60)
                                                                  CTGACCCGTGAACGTGCAGGCTTCGAAGATCGATACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCATCAAGGTCCGAACATCGGTCGGATCAACTCACAGTACGTGTACGCACATACTAACGAATACAGCTTCCTTC                                                                                 >  NB501016:230:HFVJLAFXY:1:11305:23287:3461/1‑151 (MQ=60)
                                                                    GACCCGTGACCGTGCAGGCTTCGACGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAAGCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGA                                                                                <  NB501016:230:HFVJLAFXY:2:11207:11557:19297/150‑1 (MQ=60)
                                                                    GACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTG                                                                                 <  NB501016:230:HFVJLAFXY:4:11504:17943:7601/149‑1 (MQ=60)
                                                                    GACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTT                                                                                  <  NB501016:230:HFVJLAFXY:3:11604:15100:10105/148‑1 (MQ=60)
                                                                    GACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTA                                                                                                              <  NB501016:230:HFVJLAFXY:2:21112:12650:17319/120‑1 (MQ=60)
                                                                    GACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAG                                                                               <  NB501016:230:HFVJLAFXY:4:21408:15973:2475/151‑1 (MQ=60)
                                                                      CCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGACTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGAC                                                                             >  NB501016:230:HFVJLAFXY:3:21406:10845:19124/1‑151 (MQ=60)
                                                                        CGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCC                                                                                                                   >  NB501016:230:HFVJLAFXY:1:21307:24579:11821/1‑111 (MQ=60)
                                                                           GAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCG                                                                         <  NB501016:230:HFVJLAFXY:1:11105:12971:10524/150‑1 (MQ=60)
                                                                            AACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTA                                                                       <  NB501016:230:HFVJLAFXY:1:11102:3691:19116/151‑1 (MQ=60)
                                                                              CGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGAC                                                                                                     >  NB501016:230:HFVJLAFXY:2:21203:23462:6468/1‑119 (MQ=60)
                                                                               ggagttcagaggtgtgctgttccgatgtCCCGACTCACTACGGTCGCGTATGTCCAATCGAACCCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTATGTCCGTGTACGAACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCG                                                                    <  NB501016:230:HFVJLAFXY:2:11106:8106:8695/123‑1 (MQ=60)
                                                                                ccgattCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCG                                                                    <  NB501016:230:HFVJLAFXY:3:21408:19497:15599/144‑1 (MQ=60)
                                                                                 GCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGT                                                                   >  NB501016:230:HFVJLAFXY:1:11102:4260:5024/1‑150 (MQ=60)
                                                                                 GCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTA                                                                  <  NB501016:230:HFVJLAFXY:1:21112:16686:11405/151‑1 (MQ=60)
                                                                                    GGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGCCTCCGTATCGTAA                                                                 >  NB501016:230:HFVJLAFXY:1:11207:22123:3476/1‑149 (MQ=60)
                                                                                      CTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAG                                                                               <  NB501016:230:HFVJLAFXY:3:21411:23076:6948/133‑1 (MQ=60)
                                                                                      CTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACACCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTTTCCTTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTG                                                             >  NB501016:230:HFVJLAFXY:2:11104:2437:16100/1‑151 (MQ=60)
                                                                                        TCGAAGTGCGAGACGTAGACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAGCATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTG                                                             <  NB501016:230:HFVJLAFXY:2:21201:22894:20019/149‑1 (MQ=60)
                                                                                                         CACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCG                                                                    >  NB501016:230:HFVJLAFXY:4:11503:19197:18315/1‑125 (MQ=60)
                                                                                                           CCCGACTCACTAGGGTAGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGAC                                        <  NB501016:230:HFVJLAFXY:2:11107:4708:19255/151‑1 (MQ=60)
                                                                                                             CGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACG                                       >  NB501016:230:HFVJLAFXY:2:21205:8702:17893/1‑150 (MQ=60)
                                                                                                                CTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAAT                                   >  NB501016:230:HFVJLAFXY:3:11612:13389:12384/1‑151 (MQ=60)
                                                                                                                  CACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTC                                 >  NB501016:230:HFVJLAFXY:4:11505:24276:2786/1‑151 (MQ=60)
                                                                                                                  CACTACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGGTGTAACTGACGAAATTC                                 >  NB501016:230:HFVJLAFXY:1:11210:24414:13280/1‑151 (MQ=60)
                                                                                                                  CACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCACCTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCGTCCTTGAGCCTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTC                                 >  NB501016:230:HFVJLAFXY:1:21302:22947:2582/1‑151 (MQ=60)
                                                                                                                  CACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATA                                                                                            <  NB501016:230:HFVJLAFXY:1:11306:9535:18287/92‑1 (MQ=60)
                                                                                                                    CTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATC                                                                     >  NB501016:230:HFVJLAFXY:4:21608:11914:17862/1‑113 (MQ=60)
                                                                                                                      ccgagctCGTATGTCCAATCGAAACCCCTGACGGTCCGAAACTCGGTCTGATCAACTCTCTGTCCGTGTACGAACAGAGTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACT                              <  NB501016:230:HFVJLAFXY:3:11403:24558:20129/143‑1 (MQ=60)
                                                                                                                      ACGGTCGCGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACT                              >  NB501016:230:HFVJLAFXY:1:21109:21708:8416/1‑150 (MQ=60)
                                                                                                                        GGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACC                           <  NB501016:230:HFVJLAFXY:2:21306:17981:6033/151‑1 (MQ=60)
                                                                                                                             CGTATGTCCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTC                                 <  NB501016:230:HFVJLAFXY:3:21510:19131:1258/140‑1 (MQ=60)
                                                                                                                                    CCAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCG               >  NB501016:230:HFVJLAFXY:2:21207:9520:12017/1‑151 (MQ=60)
                                                                                                                                     CAATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCG               >  NB501016:230:HFVJLAFXY:3:21411:21637:8508/1‑150 (MQ=60)
                                                                                                                                       ATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGA              >  NB501016:230:HFVJLAFXY:2:11312:16575:9371/1‑149 (MQ=60)
                                                                                                                                       ATCGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGCAACTGACGAAATTCACTACCTGTCTGCTATCGA              >  NB501016:230:HFVJLAFXY:2:21309:16969:15078/1‑149 (MQ=60)
                                                                                                                                       ATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGA              >  NB501016:230:HFVJLAFXY:4:11605:18579:10883/1‑149 (MQ=60)
                                                                                                                                         CGAAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACAGACGAAATTCACTACCTGTCTGCTATCGAAGA           >  NB501016:230:HFVJLAFXY:1:21305:15921:7154/1‑150 (MQ=60)
                                                                                                                                          GAAACCCCTGCAGGTCCGAACATCGGTCTGATCTACTCTCTGTCCGTGTACGCACAGACTAACTAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACTGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAG         <  NB501016:230:HFVJLAFXY:2:11211:7227:19407/151‑1 (MQ=60)
                                                                                                                                           AACCCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGG        <  NB501016:230:HFVJLAFXY:4:11604:13095:16388/151‑1 (MQ=60)
                                                                                                                                           AAACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGG        >  NB501016:230:HFVJLAFXY:4:11410:21645:15048/1‑151 (MQ=60)
                                                                                                                                            AACCCCTGCAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGC       <  NB501016:230:HFVJLAFXY:3:11512:21317:4908/151‑1 (MQ=60)
                                                                                                                                              CCCCTGAAGGTCCGAACATCGGTCTGATCATCTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCA      >  NB501016:230:HFVJLAFXY:2:21108:14030:17360/1‑150 (MQ=60)
                                                                                                                                              CCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCA      >  NB501016:230:HFVJLAFXY:3:21609:22927:2158/1‑150 (MQ=60)
                                                                                                                                                   GAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTA  <  NB501016:230:HFVJLAFXY:1:21207:15209:15778/149‑1 (MQ=60)
                                                                                                                                                    CAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCAC                               <  NB501016:230:HFVJLAFXY:2:21308:9199:5350/119‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
GACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACGGTGTTGTAACTGACGAAATTCACTACCTGTCTGCTATCGAAGAAGGCAACTA  >  NC_000913/4182790‑4183085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑