Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F36 I0 R1 141 81.4 2739672 97.2% 2662961 142.9

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTC  >  NC_000913/4296231‑4296529
                                                                                                                                                          |                                                                                                                                                      
GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATGTAGCGAGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C                                                                                                                                                        >  NB501016:230:HFVJLAFXY:3:11506:14229:20152/1‑151 (MQ=0)
GAATTAGCGTGATTTTGCAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGAGAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C                                                                                                                                                        <  NB501016:230:HFVJLAFXY:3:11608:13690:7726/151‑1 (MQ=0)
                                                       CGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CGACGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTA                                                                                                  >  NB501016:230:HFVJLAFXY:3:21402:7421:7850/1‑150 (MQ=0)
                                                            GATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTA                                                                                             >  NB501016:230:HFVJLAFXY:2:11111:5964:9819/1‑150 (MQ=0)
                                                               GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCG                                                                                         <  NB501016:230:HFVJLAFXY:3:21410:4033:1030/151‑1 (MQ=5)
                                                                   CGCTTAACTCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGG                                                                                     <  NB501016:230:HFVJLAFXY:3:11507:15191:16080/151‑1 (MQ=16)
                                                                        AACGCGACTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGAGTGATTTTGTAGGTCGGCTAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGAGAGGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAT                                                                                <  NB501016:230:HFVJLAFXY:3:11612:13075:3036/151‑1 (MQ=23)
                                                                             GTCTTATCAGGCCTCCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTTCTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTAAACCGTTGGTTAATTTTTC                                                                           >  NB501016:230:HFVJLAFXY:3:21507:6687:4053/1‑151 (MQ=24)
                                                                                 TATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCCGTCGGATAAGTCGTTTCCG‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCATATCAGGCCTATCTTAACCGTTGGTTAATTATTCAAAC                                                                       >  NB501016:230:HFVJLAFXY:1:11107:13622:19927/1‑151 (MQ=55)
                                                                                 TATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAC                                                                       >  NB501016:230:HFVJLAFXY:3:11606:5809:6137/1‑151 (MQ=45)
                                                                                       GCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCA                                                                 <  NB501016:230:HFVJLAFXY:1:11204:25190:6452/151‑1 (MQ=57)
                                                                                          TACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAC                                                              >  NB501016:230:HFVJLAFXY:1:21209:13160:8846/1‑151 (MQ=60)
                                                                                           ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACC                                                             >  NB501016:230:HFVJLAFXY:1:21210:23208:19602/1‑151 (MQ=60)
                                                                                           ACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACA                                                               <  NB501016:230:HFVJLAFXY:4:21411:8566:15249/149‑1 (MQ=60)
                                                                                                     GCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAA                                                    >  NB501016:230:HFVJLAFXY:3:11503:6426:11094/1‑150 (MQ=60)
                                                                                                        CAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTC                                                                           >  NB501016:230:HFVJLAFXY:1:21111:17622:12411/1‑124 (MQ=39)
                                                                                                           AAGCCTGATTTAGCGGGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTC                                             <  NB501016:230:HFVJLAFXY:2:21205:11788:4064/151‑1 (MQ=60)
                                                                                                              CCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCAT                                          <  NB501016:230:HFVJLAFXY:1:11108:18799:3040/151‑1 (MQ=60)
                                                                                                                      AGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTC                                   >  NB501016:230:HFVJLAFXY:2:11112:22104:16651/1‑150 (MQ=60)
                                                                                                                         GTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGT                               >  NB501016:230:HFVJLAFXY:3:11512:9008:17944/1‑151 (MQ=60)
                                                                                                                           GATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTC                             <  NB501016:230:HFVJLAFXY:2:11103:21164:8863/151‑1 (MQ=60)
                                                                                                                                TGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACC                         <  NB501016:230:HFVJLAFXY:3:11609:18679:16657/150‑1 (MQ=60)
                                                                                                                                    GGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAG                       <  NB501016:230:HFVJLAFXY:4:11603:14038:15225/148‑1 (MQ=60)
                                                                                                                                        GGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTT                                                                   <  NB501016:230:HFVJLAFXY:3:11411:2836:3514/100‑1 (MQ=60)
                                                                                                                                        GGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCATAACAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCG                >  NB501016:230:HFVJLAFXY:1:11101:3552:3725/1‑151 (MQ=60)
                                                                                                                                                 GTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCT       <  NB501016:230:HFVJLAFXY:3:11603:22401:2711/151‑1 (MQ=60)
                                                                                                                                                 GTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCT       <  NB501016:230:HFVJLAFXY:4:11505:6225:3218/151‑1 (MQ=60)
                                                                                                                                                 GTTT‑‑ACGC‑‑CGCATCCGACATCAAGGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCT       <  NB501016:230:HFVJLAFXY:1:21303:26528:3730/151‑1 (MQ=60)
                                                                                                                                                        cgC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTC  >  NB501016:230:HFVJLAFXY:4:11412:2752:18284/3‑151 (MQ=60)
                                                                                                                                                          |                                                                                                                                                      
GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTC  >  NC_000913/4296231‑4296529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑