Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F36 I0 R1 111 89.1 3136336 98.1% 3076745 142.5

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGT  >  NC_000913/4296231‑4296527
                                                                                                                                                        |                                                                                                                                                  
gtgactggagttcagacgtgtgctttccgatctTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C                                                                                                                                                    <  NB501016:230:HFVJLAFXY:2:11111:9044:13794/118‑1 (MQ=0)
                             GGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGGCTCTTTTAGCGTTATTTTGTAGTACGGATAAGGCGTTTA‑‑CCccgaataagagatgaatgcctgatg                                                                                                                          >  NB501016:230:HFVJLAFXY:1:21105:1281:11574/1‑123 (MQ=0)
                                                                  ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTG                                                                                    <  NB501016:230:HFVJLAFXY:4:11603:14310:16541/151‑1 (MQ=11)
                                                                     CTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT                                                                                 >  NB501016:230:HFVJLAFXY:4:11505:2520:11719/1‑151 (MQ=20)
                                                                          CGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTTTACTCAGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTT                                                                            <  NB501016:230:HFVJLAFXY:3:21410:9844:5932/151‑1 (MQ=38)
                                                                                 TATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA                                                                       <  NB501016:230:HFVJLAFXY:1:11104:12769:18413/149‑1 (MQ=41)
                                                                                  ATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAC                                                                     <  NB501016:230:HFVJLAFXY:4:11408:10134:7221/150‑1 (MQ=45)
                                                                                     AGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTT                                                                 >  NB501016:230:HFVJLAFXY:4:21609:7090:11962/1‑151 (MQ=53)
                                                                                            CGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTagatcggaagagcacacgtctggaact                                                          >  NB501016:230:HFVJLAFXY:1:11111:15028:20365/1‑124 (MQ=10)
                                                                                              CCAGTCTGCGCAATAGGCTGATTTAGCGTGAGTTTGTAGGGCGGATATGGCGTTTACGCGGCATCCGACATCAATTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGG                                                        <  NB501016:230:HFVJLAFXY:1:11312:1036:2195/151‑1 (MQ=60)
                                                                                              CCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACGTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGG                                                        <  NB501016:230:HFVJLAFXY:4:11403:6798:10258/151‑1 (MQ=60)
                                                                                              CCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGG                                                        >  NB501016:230:HFVJLAFXY:3:11611:6871:10995/1‑151 (MQ=60)
                                                                                               CAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGT                                                       >  NB501016:230:HFVJLAFXY:3:21506:10733:13819/1‑151 (MQ=60)
                                                                                                AGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGT                                                       >  NB501016:230:HFVJLAFXY:3:21502:18603:17426/1‑150 (MQ=60)
                                                                                                 GACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTAT                                                     >  NB501016:230:HFVJLAFXY:2:11206:1275:9045/1‑151 (MQ=60)
                                                                                                  ACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATC                                                    <  NB501016:230:HFVJLAFXY:4:21401:2680:12617/151‑1 (MQ=60)
                                                                                                  ACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATC                                                    <  NB501016:230:HFVJLAFXY:4:11605:10218:1982/151‑1 (MQ=60)
                                                                                                        CAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCC                                              >  NB501016:230:HFVJLAFXY:4:21409:15200:2884/1‑151 (MQ=60)
                                                                                                            AGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCC                                          <  NB501016:230:HFVJLAFXY:3:21408:2769:6200/151‑1 (MQ=60)
                                                                                                            AGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTC                                           <  NB501016:230:HFVJLAFXY:1:21106:18780:8997/150‑1 (MQ=60)
                                                                                                             GCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCA                                         <  NB501016:230:HFVJLAFXY:3:21504:17990:20226/151‑1 (MQ=60)
                                                                                                             GCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAC                                                                     >  NB501016:230:HFVJLAFXY:1:21311:8909:11569/1‑123 (MQ=48)
                                                                                                              CCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTCGTTAATTTTTCAAACTCTTAACACCCGGTATCAAACCCTTCCAT                                        >  NB501016:230:HFVJLAFXY:4:21607:9383:4688/1‑151 (MQ=60)
                                                                                                               CTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTT                                                                                     >  NB501016:230:HFVJLAFXY:3:11512:7766:4256/1‑105 (MQ=11)
                                                                                                                 GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATA                                       <  NB501016:230:HFVJLAFXY:4:21605:21250:5940/149‑1 (MQ=60)
                                                                                                                   TTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCA                                                   >  NB501016:230:HFVJLAFXY:3:11606:10248:4322/1‑135 (MQ=60)
                                                                                                                        CGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCTGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGG                              >  NB501016:230:HFVJLAFXY:2:21112:15761:7818/1‑151 (MQ=60)
                                                                                                                        CGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGG                              >  NB501016:230:HFVJLAFXY:1:21203:5568:8961/1‑151 (MQ=60)
                                                                                                                                 GTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTC                                                                   <  NB501016:230:HFVJLAFXY:1:11210:3181:12626/105‑1 (MQ=56)
                                                                                                                                    GGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGC                  >  NB501016:230:HFVJLAFXY:2:21207:11786:20367/1‑151 (MQ=60)
                                                                                                                                    GGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAG                                    <  NB501016:230:HFVJLAFXY:2:21207:15878:1693/133‑1 (MQ=60)
                                                                                                                                     GTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGC                  >  NB501016:230:HFVJLAFXY:3:21404:17341:15462/1‑150 (MQ=60)
                                                                                                                                       CGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGC               >  NB501016:230:HFVJLAFXY:2:11105:3738:3165/1‑151 (MQ=60)
                                                                                                                                       CGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTACAGC               >  NB501016:230:HFVJLAFXY:3:21607:3782:12206/1‑151 (MQ=60)
                                                                                                                                             AGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAATTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGATCAACCAGAGCAGAGCCTTT         >  NB501016:230:HFVJLAFXY:3:11606:22487:10235/1‑151 (MQ=60)
                                                                                                                                              GGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTT        <  NB501016:230:HFVJLAFXY:3:21504:4718:4813/151‑1 (MQ=60)
                                                                                                                                               gcgttttctCCGCATCCGACATCAATGCCTGATGCTTCTCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACGCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTG       <  NB501016:230:HFVJLAFXY:1:11110:16908:4497/142‑1 (MQ=60)
                                                                                                                                                   ttacgCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTG   >  NB501016:230:HFVJLAFXY:1:11210:17491:17728/6‑151 (MQ=60)
                                                                                                                                                    tacgCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAagatcggaagagcacaacgtctgaactccagtcac    >  NB501016:230:HFVJLAFXY:2:21211:17080:6906/5‑114 (MQ=60)
                                                                                                                                                     acgCCGCATCCGAGATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGT  <  NB501016:230:HFVJLAFXY:4:21502:16235:17552/147‑1 (MQ=60)
                                                                                                                                                        |                                                                                                                                                  
GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGT  >  NC_000913/4296231‑4296527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑