Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F16 I1 R1
|
10 |
35.0 |
1256272 |
95.4% |
1198483 |
136.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,284,630 |
G→A |
L85L (CTG→CTA) |
yejM → |
essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,284,630 | 0 | G | A | 100.0%
| 148.7
/ NA
| 44 | L85L (CTG→CTA) | yejM | essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/23); total (21/23) |
ACCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTGATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGC > NC_000913/2284485‑2284770
|
aCCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCtaat > 2:73074/1‑148 (MQ=255)
cTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAg < 5:28214/149‑1 (MQ=255)
tttATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGtt < 1:73074/149‑1 (MQ=255)
tCGCCGACTGGCCGTCAACGCTTGCTGGTCGCATTTATTCCTAGGTATGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGTTCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCtaat < 1:135455/139‑1 (MQ=255)
tCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTg > 1:87560/1‑149 (MQ=255)
ccGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTcc < 2:33720/149‑1 (MQ=255)
gCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTc > 4:145353/1‑149 (MQ=255)
gCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAAcgg > 8:128943/1‑149 (MQ=255)
gCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAAcgg > 2:5285/1‑149 (MQ=255)
gCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAAcggcgg < 1:5285/148‑1 (MQ=255)
tGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGaa > 6:80535/1‑149 (MQ=255)
ggTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAAt < 2:87560/149‑1 (MQ=255)
aTTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGc > 1:49566/1‑148 (MQ=255)
cGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATc < 5:80535/148‑1 (MQ=255)
gTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCg > 3:15963/1‑148 (MQ=255)
cATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGc > 7:120932/1‑148 (MQ=255)
ttctCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGaa < 8:86687/146‑1 (MQ=255)
ttATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGaa < 2:49566/149‑1 (MQ=255)
ggCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCtt < 7:128943/149‑1 (MQ=255)
ccATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTa < 4:24702/149‑1 (MQ=255)
tttCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGaa > 1:91893/1‑113 (MQ=255)
tttCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGaa < 2:91893/113‑1 (MQ=255)
tttCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTc > 1:49328/1‑149 (MQ=255)
ccTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTtcca < 3:145353/147‑1 (MQ=255)
gtgtTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCg < 4:26374/124‑1 (MQ=255)
gtgtTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCg > 3:26374/1‑124 (MQ=255)
tgtTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTtccatctccat < 2:110079/149‑1 (MQ=255)
ttCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTccatctccatct < 2:49328/149‑1 (MQ=255)
ccTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATcc > 5:70309/1‑148 (MQ=255)
cTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATc < 4:15963/148‑1 (MQ=255)
gCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTc < 8:120932/148‑1 (MQ=255)
cTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTccatctccatct < 3:82276/134‑1 (MQ=255)
cTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTccatctccatct > 4:82276/1‑134 (MQ=255)
aTCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCGATTACTGATCGATTGAGAAGTCTTTACTAGTTTCCATCTCCATAATAATCCCATAGTCTGGc > 8:77247/1‑148 (MQ=255)
gatttCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACt < 1:99529/146‑1 (MQ=255)
cccGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGtt < 7:77247/148‑1 (MQ=255)
cGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCtt < 8:91415/100‑1 (MQ=255)
cGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCtt > 7:91415/1‑100 (MQ=255)
aCCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTg > 8:135191/1‑138 (MQ=255)
aCCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGAGCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTg < 7:135191/138‑1 (MQ=255)
cTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCaa < 7:82982/144‑1 (MQ=255)
cTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCaa > 8:82982/1‑144 (MQ=255)
cTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAAcc > 3:51059/1‑146 (MQ=255)
aTCGTCGGCTCCCAGAGGCTAAAAAGGTTTTTGTCCGTAATTCTGGCAACGGCGGGAATTACGCTATTAAAGATCGAAATCGAAGTCTTTACACGTTTCCATATACATCTTAATACCATAGTCt > 2:76592/1‑124 (MQ=255)
tCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGAt > 2:157916/1‑149 (MQ=255)
cAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCATAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGgc > 7:97084/1‑149 (MQ=255)
|
ACCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTGATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGC > NC_000913/2284485‑2284770
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTGATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGCGCGCGACTGG > NC_000913/2284486‑2284780
|
CCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGG < NB501016:228:HFK2FAFXY:3:11501:24311:14097/151‑1 (MQ=60)
GTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTT < NB501016:228:HFK2FAFXY:1:11306:2218:6649/151‑1 (MQ=60)
ATCGCCGACTGGCCGTCAACGCTTGCTGGTCGCATTTATTCCTAGGTATGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGTTCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATG < NB501016:228:HFK2FAFXY:1:21207:2897:14185/141‑1 (MQ=60)
ATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGT > NB501016:228:HFK2FAFXY:1:11312:17028:3762/1‑151 (MQ=60)
TGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGG < NB501016:228:HFK2FAFXY:1:11103:1924:2789/150‑1 (MQ=60)
CATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCT > NB501016:228:HFK2FAFXY:1:11208:9328:14643/1‑150 (MQ=60)
ATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTAGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACT < NB501016:228:HFK2FAFXY:1:21102:4874:17898/151‑1 (MQ=60)
ACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCG < NB501016:228:HFK2FAFXY:3:11612:4822:5746/150‑1 (MQ=60)
CGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATAGA > NB501016:228:HFK2FAFXY:2:11109:23108:2359/1‑150 (MQ=60)
GCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCG > NB501016:228:HFK2FAFXY:4:21601:13770:6472/1‑150 (MQ=60)
CGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTT < NB501016:228:HFK2FAFXY:4:21605:15422:9489/151‑1 (MQ=60)
ATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCG > NB501016:228:HFK2FAFXY:1:11208:2856:12989/1‑151 (MQ=60)
ATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAAT > NB501016:228:HFK2FAFXY:1:21101:13940:17673/1‑115 (MQ=60)
TCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCAT < NB501016:228:HFK2FAFXY:2:21312:11621:5073/149‑1 (MQ=60)
GGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGA > NB501016:228:HFK2FAFXY:2:11202:7453:8886/1‑126 (MQ=60)
GTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTACGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTGTACTCGTTTCCATCTCCATCAT > NB501016:228:HFK2FAFXY:4:11512:11267:20295/1‑151 (MQ=60)
ACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCC > NB501016:228:HFK2FAFXY:3:11607:16562:17531/1‑150 (MQ=60)
gttctgaggtgtgctcttccgttctttTCGTCGGCTCCCAGAGGCTTATGAGGTTTTTGTCCGTGATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGTTGTCTTTACTCGTTTCCATCTGCATCTTAATCCCATCGTCTG < NB501016:228:HFK2FAFXY:1:11307:1590:14993/124‑1 (MQ=60)
GCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTT < NB501016:228:HFK2FAFXY:2:21104:11214:17479/136‑1 (MQ=60)
cgatTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTG < NB501016:228:HFK2FAFXY:1:21104:6138:19077/147‑1 (MQ=60)
TCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTA < NB501016:228:HFK2FAFXY:4:21403:20535:10151/150‑1 (MQ=60)
CCGCTGACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTT > NB501016:228:HFK2FAFXY:4:21410:15193:2523/1‑102 (MQ=60)
GACCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGAGCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGG < NB501016:228:HFK2FAFXY:4:21608:22692:16095/140‑1 (MQ=60)
CCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCC > NB501016:228:HFK2FAFXY:2:11302:20085:8468/1‑148 (MQ=60)
CCTTTATCGTCGGCTCCCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAAC < NB501016:228:HFK2FAFXY:4:21406:20342:4811/146‑1 (MQ=60)
CCAGAGGCTAATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCATAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGCG > NB501016:228:HFK2FAFXY:4:21412:22480:12209/1‑151 (MQ=60)
AATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGCGCGCGACTGG > NB501016:228:HFK2FAFXY:4:11611:25741:8153/1‑151 (MQ=60)
AATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATAAACCCAGACGAAAATGAGATGGCGCGCGACTGG > NB501016:228:HFK2FAFXY:1:21211:11350:8798/1‑151 (MQ=60)
|
CCTGTTTATCGCCGACTGGCCGACAACGCTTGCTGGTCGCATTTATTCCTACGTAAGCATTATCGGCCATTTCAGCTTCCTGGTGTTCGCCACCTACTTGCTGATCCTCTTCCCGCTGACCTTTATCGTCGGCTCCCAGAGGCTGATGAGGTTTTTGTCCGTCATTCTGGCAACGGCGGGAATGACGCTATTACTGATCGATAGCGAAGTCTTTACTCGTTTCCATCTCCATCTTAATCCCATCGTCTGGCAACTGGTTATCAACCCAGACGAAAATGAGATGGCGCGCGACTGG > NC_000913/2284486‑2284780
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
