Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F36 I1 R1 14 89.5 2915230 98.1% 2859840 144.1

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑CGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAG  >  NC_000913/762780‑763068
                                                                                                                                                   |                                                                                                                                               
TATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑GGG                                                                                                                                             >  NB501016:230:HFVJLAFXY:3:11511:24236:8234/1‑151 (MQ=60)
    GCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAgggggtg                                                                                                                                          >  NB501016:230:HFVJLAFXY:4:11411:25476:19051/1‑144 (MQ=60)
    GCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAgggggtg                                                                                                                                          <  NB501016:230:HFVJLAFXY:4:21401:17661:16553/151‑8 (MQ=60)
        TCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACC                                                                                                                                      <  NB501016:230:HFVJLAFXY:2:21201:4485:16913/150‑1 (MQ=60)
                    GAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGG                                                                                                                          >  NB501016:230:HFVJLAFXY:2:21105:23241:18758/1‑150 (MQ=60)
                     AAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGA                                                                                                                         >  NB501016:230:HFVJLAFXY:2:11211:13642:7679/1‑150 (MQ=60)
                       GCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGC                                                                                                                      >  NB501016:230:HFVJLAFXY:2:11102:13990:10192/1‑151 (MQ=60)
                        cctgataagacgcgtcagcgtcgcATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGCCAATGCCCGGAAGCG                                                                                                                     >  NB501016:230:HFVJLAFXY:4:11603:4929:1966/25‑151 (MQ=60)
                                GACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAA                                                                                                             <  NB501016:230:HFVJLAFXY:4:21407:21738:5757/151‑1 (MQ=60)
                                 ACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAAT                                                                                                            <  NB501016:230:HFVJLAFXY:1:21203:20892:9771/151‑1 (MQ=60)
                                      GACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑GGGTGTGACCGACAATGCCCGGAAGCGATACGAAATATTCG                                                                                                       <  NB501016:230:HFVJLAFXY:1:21307:1189:7860/151‑1 (MQ=60)
                                      GACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGGCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCG                                                                                                       <  NB501016:230:HFVJLAFXY:1:21308:26725:7849/151‑1 (MQ=60)
                                       ACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGG                                                                                                      <  NB501016:230:HFVJLAFXY:2:11208:20479:11753/151‑1 (MQ=60)
                                                ATCAGGCCTACGGGACCACCAATGGAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTT                                                                                             <  NB501016:230:HFVJLAFXY:3:11507:6099:9527/151‑1 (MQ=60)
                                                   AGGCCTACGGGTCCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTA                                                                                           >  NB501016:230:HFVJLAFXY:2:11104:24008:16926/1‑150 (MQ=60)
                                                   AGGCATAAGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGATTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAA                                                                                          <  NB501016:230:HFVJLAFXY:1:21309:22892:12708/151‑1 (MQ=60)
                                                    GGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGTTGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAA                                                                                          >  NB501016:230:HFVJLAFXY:3:11608:5747:16065/1‑150 (MQ=60)
                                                      CCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAG                                                                                       <  NB501016:230:HFVJLAFXY:1:11306:6922:16256/151‑1 (MQ=60)
                                                      CCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAG                                                                                       <  NB501016:230:HFVJLAFXY:3:21402:9898:18084/151‑1 (MQ=60)
                                                            GGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTC                                                                                                        <  NB501016:230:HFVJLAFXY:2:11309:2245:10713/128‑1 (MQ=60)
                                                               CCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATT                                                                              <  NB501016:230:HFVJLAFXY:1:21204:6837:12063/151‑1 (MQ=60)
                                                                    AATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGA                                                                         <  NB501016:230:HFVJLAFXY:1:11102:14592:11293/151‑1 (MQ=60)
                                                                      TGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGA                                                                         >  NB501016:230:HFVJLAFXY:2:11310:22526:17144/1‑149 (MQ=60)
                                                                       GTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAG                                                                       <  NB501016:230:HFVJLAFXY:1:11305:4636:5942/150‑1 (MQ=60)
                                                                         AGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGAT                                                                    >  NB501016:230:HFVJLAFXY:1:11205:13192:17980/1‑151 (MQ=60)
                                                                           GTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAATAGATAACGATTACTGAAGGATGG                                                                  >  NB501016:230:HFVJLAFXY:4:11601:21951:19442/1‑151 (MQ=60)
                                                                              GGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGAC                                                                >  NB501016:230:HFVJLAFXY:1:21104:17908:1145/1‑150 (MQ=60)
                                                                              GGATAACGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTT                                                                                             <  NB501016:230:HFVJLAFXY:1:11207:11797:12863/121‑1 (MQ=60)
                                                                                ATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATG                                                                   >  NB501016:230:HFVJLAFXY:1:11106:23069:17714/1‑145 (MQ=60)
                                                                                  AAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAAC                                                           <  NB501016:230:HFVJLAFXY:3:11610:15557:12293/151‑1 (MQ=60)
                                                                                    GGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTGTAACGTGTCTTATCAGGCCGAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAA                                                                                          >  NB501016:230:HFVJLAFXY:4:21510:26101:2621/1‑118 (MQ=60)
                                                                                      CGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAAC                                                                                  >  NB501016:230:HFVJLAFXY:2:11105:5115:15751/1‑124 (MQ=60)
                                                                                       gcttgcgccttATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATG                                                      <  NB501016:230:HFVJLAFXY:4:11408:15286:15109/140‑1 (MQ=60)
                                                                                                GCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACAT                                             >  NB501016:230:HFVJLAFXY:3:21510:2732:5965/1‑151 (MQ=60)
                                                                                                      GACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATG                                                      <  NB501016:230:HFVJLAFXY:4:11509:6597:11424/136‑1 (MQ=60)
                                                                                                         AAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAG                                    <  NB501016:230:HFVJLAFXY:1:11208:11886:19640/151‑1 (MQ=60)
                                                                                                          AGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGG                                   >  NB501016:230:HFVJLAFXY:4:11403:1176:3739/1‑151 (MQ=60)
                                                                                                                 CCTGATGTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAAC                             >  NB501016:230:HFVJLAFXY:3:21504:13284:4539/1‑150 (MQ=60)
                                                                                                                       GTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTT                      <  NB501016:230:HFVJLAFXY:4:11505:5361:6036/151‑1 (MQ=60)
                                                                                                                       GTGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTT                      >  NB501016:230:HFVJLAFXY:2:11110:11261:19539/1‑151 (MQ=60)
                                                                                                                        TGACGTTTAACGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTT                     >  NB501016:230:HFVJLAFXY:4:11404:21449:16247/1‑151 (MQ=60)
                                                                                                                                  CGTGTCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATG           <  NB501016:230:HFVJLAFXY:2:21310:16180:2906/151‑1 (MQ=60)
                                                                                                                                      TCTTATCAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTT       >  NB501016:230:HFVJLAFXY:2:11310:10680:6650/1‑151 (MQ=60)
                                                                                                                                            CAGGCCTAGGGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAG  <  NB501016:230:HFVJLAFXY:1:11202:11165:5424/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                               
TATCGCCTTCTCCGGCAATTGAAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGGGACCACCAATGTAGGTCGGATAAGGCGCAAGCGCCGCATCCGACAAGCGATGCCTGATGTGACGTTTAACGTGTCTTATCAGGCCTA‑CGG‑GTGACCGACAATGCCCGGAAGCGATACGAAATATTCGGTCTACGGTTTAAAAGATAACGATTACTGAAGGATGGACAGAACACATGAACTTACATGAATATCAGGCAAAACAACTTTTTGCCCGCTATGGCTTACCAG  >  NC_000913/762780‑763068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑