Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F33 I1 R1
|
23 |
35.1 |
1378992 |
95.0% |
1310042 |
124.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,661,691 |
C→A |
A147A (GCG→GCT) |
iscR ← |
isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,661,691 | 0 | C | A | 100.0%
| 50.8
/ NA
| 16 | A147A (GCG→GCT) | iscR | isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/7); total (9/7) |
CGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCC > NC_000913/2661546‑2661835
|
cGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGgagc < 8:50661/148‑1 (MQ=255)
tCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTAt > 1:43235/1‑149 (MQ=255)
tACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTTCGGGTGCGTGGAGATTCGTGAGTAAGCTGACGACCAGACACATc > 3:10557/1‑149 (MQ=255)
tACGACCGTGTATACGGAGTATTAAGCACTCCCGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTAAACGACGATCGCGTCTTGTGTCCGGGTGCGAGGACCGTCGTGAGTATGCTGCCGACCAGACACATCCAGGACTTCCTg > 8:154379/1‑148 (MQ=255)
aCGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGt > 6:32063/1‑149 (MQ=255)
cGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGt < 5:32063/148‑1 (MQ=255)
aCCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGa > 6:164266/1‑109 (MQ=255)
aCCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCCGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGa < 5:164266/109‑1 (MQ=255)
gtTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTAATAAc > 6:80158/1‑148 (MQ=255)
cGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGtt < 2:43235/148‑1 (MQ=255)
cGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTAATAACCAggaa < 5:80158/149‑4 (MQ=255)
tCCGGCCGGAGTCTGAAGTCTTTTTATTAAGCGCGTAACTTAACGTCGCTCGCGGCTTGTGTGCGGGTGCGTGGAGCGGCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGtt < 7:30723/133‑1 (MQ=255)
aGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTc > 5:100400/1‑148 (MQ=255)
gcgTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGAc < 5:9516/137‑1 (MQ=255)
gcgTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGAc > 6:9516/1‑137 (MQ=255)
gcgTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAAt < 6:100400/149‑1 (MQ=255)
gTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGccc > 1:72951/1‑149 (MQ=255)
|
CGTACATCACTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCC > NC_000913/2661546‑2661835
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCT > NC_000913/2661555‑2661836
|
CTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATG > NB501016:228:HFK2FAFXY:1:11204:7702:10458/1‑151 (MQ=60)
AGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGC < NB501016:228:HFK2FAFXY:2:21207:13823:9931/123‑1 (MQ=60)
GGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGA > NB501016:228:HFK2FAFXY:3:11405:24956:15598/1‑120 (MQ=60)
CTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTTCGGGTGCGTGGAGATTCGTGAGTAAGCTGACGACCAGACACATCC > NB501016:228:HFK2FAFXY:2:11105:2673:19116/1‑151 (MQ=60)
ACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTT < NB501016:228:HFK2FAFXY:3:11501:20009:13026/150‑1 (MQ=60)
GACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCCGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAG < NB501016:228:HFK2FAFXY:3:21606:2444:7727/111‑1 (MQ=60)
ACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTAATAACCAGgaag < NB501016:228:HFK2FAFXY:3:11608:11121:5305/151‑5 (MQ=60)
CTCCGGCCGGAGTCTGAAGTCTTTTTATTAAGCGCGTAACTTAACGTCGCTCGCGGCTTGTGTGCGGGTGCGTGGAGCGGCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTC < NB501016:228:HFK2FAFXY:4:11502:25688:1106/135‑1 (MQ=60)
GAATTCTTTTTATTAAGCTCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAG < NB501016:228:HFK2FAFXY:1:11111:23133:9285/128‑1 (MQ=60)
AAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGCCACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCA > NB501016:228:HFK2FAFXY:3:21404:22933:6565/1‑150 (MQ=60)
CGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGAGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACG < NB501016:228:HFK2FAFXY:3:11404:24672:15203/139‑1 (MQ=60)
CGTGGAGCGTCGTGAGTATGCTGACGCCCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCT > NB501016:228:HFK2FAFXY:1:11303:6899:10244/1‑151 (MQ=60)
|
CTCAATGCAAGGAATCAGGCTACCGGCTGGATGTACGACCGTGTTTACGGAGTATTTAGCACTCCGGCCTGATTCTGAATTCTTTTTATTAAGCGCGTAACTTAACGTCGATCGCGTCTTGTGTGCGGGTGCGTGGCGCGTCGTGAGTATGCTGACGACCAGACACATCCAGCACTTCCTGGTTATTAACCAGTTCGCCTAAAGTAATGTTGTTGAGAAAACCGGTGAGACGGTCGCTCAAATCACGCCACAGCGCGTGGGTCAGGCATTTATCGCCGCCCT > NC_000913/2661555‑2661836
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
