Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F70 I0 R1 119 101.6 3446078 97.9% 3373710 141.6

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTC  >  NC_000913/4296231‑4296529
                                                                                                                                                        |                                                                                                                                                    
catttagcctgattatgtacctcggtccAGGCGTTTATCCCGCATAAGTCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑C                                                                                                                                                      <  NB501016:230:HFVJLAFXY:3:11604:18188:5579/123‑1 (MQ=0)
                                                          CTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACTCCGCATCAGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTT                                                                                              >  NB501016:230:HFVJLAFXY:2:11308:23718:18698/1‑151 (MQ=5)
                                                               CCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGGCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCG                                                                                         <  NB501016:230:HFVJLAFXY:4:11505:15313:16594/151‑1 (MQ=5)
                                                                CGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGT                                                                                        >  NB501016:230:HFVJLAFXY:4:21402:16835:19944/1‑151 (MQ=7)
                                                                   gggttatcttgtgaTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGG                                                                                     <  NB501016:230:HFVJLAFXY:1:11207:18084:2682/137‑1 (MQ=17)
                                                                   CGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGG                                                                                     <  NB501016:230:HFVJLAFXY:1:21101:15741:4545/151‑1 (MQ=16)
                                                                    GCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGT                                                                                    <  NB501016:230:HFVJLAFXY:4:21503:17865:6893/151‑1 (MQ=18)
                                                                      TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT                                                                                   >  NB501016:230:HFVJLAFXY:4:21411:8601:14022/1‑150 (MQ=20)
                                                                      TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT                                                                                   >  NB501016:230:HFVJLAFXY:2:21301:11623:1154/1‑150 (MQ=20)
                                                                      TTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTT                                                                                   >  NB501016:230:HFVJLAFXY:3:21504:13525:1777/1‑150 (MQ=31)
                                                                             GTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTC                                                                           >  NB501016:230:HFVJLAFXY:3:21502:17352:16742/1‑151 (MQ=37)
                                                                               CTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCA                                                                          >  NB501016:230:HFVJLAFXY:1:11305:15201:9991/1‑150 (MQ=39)
                                                                                 TATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA                                                                         <  NB501016:230:HFVJLAFXY:1:21110:21359:11393/149‑1 (MQ=41)
                                                                                  ATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACT                                                                      >  NB501016:230:HFVJLAFXY:4:11609:11801:14426/1‑151 (MQ=47)
                                                                                  ATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAC                                                                       <  NB501016:230:HFVJLAFXY:1:21112:6124:17212/150‑1 (MQ=45)
                                                                                  ATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAC                                                                       <  NB501016:230:HFVJLAFXY:2:11304:5046:11918/150‑1 (MQ=45)
                                                                                      GGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTC                                                                  <  NB501016:230:HFVJLAFXY:4:11611:7374:19907/151‑1 (MQ=55)
                                                                                      GGCCTACGCCAGACAGCGCAATAGCCGGACTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTC                                                                  <  NB501016:230:HFVJLAFXY:1:11209:13985:7258/151‑1 (MQ=52)
                                                                                        CCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC                                                                >  NB501016:230:HFVJLAFXY:3:21411:6353:5017/1‑151 (MQ=59)
                                                                                              CCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGG                                                          >  NB501016:230:HFVJLAFXY:3:11403:23621:11212/1‑151 (MQ=60)
                                                                                              CCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGG                                                          >  NB501016:230:HFVJLAFXY:1:11307:1461:3921/1‑151 (MQ=60)
                                                                                                  ACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTA                                                        <  NB501016:230:HFVJLAFXY:2:11304:22283:15020/149‑1 (MQ=60)
                                                                                                            AGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCC                                            <  NB501016:230:HFVJLAFXY:2:11203:21508:12394/151‑1 (MQ=60)
                                                                                                            AGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTC                                             <  NB501016:230:HFVJLAFXY:2:21111:15072:7773/150‑1 (MQ=60)
                                                                                                                       GCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATC                                                      >  NB501016:230:HFVJLAFXY:4:11507:7918:3171/1‑130 (MQ=60)
                                                                                                                        CGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAC                                                                                           >  NB501016:230:HFVJLAFXY:3:11601:24761:3262/1‑92 (MQ=2)
                                                                                                                                TGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACC                         <  NB501016:230:HFVJLAFXY:4:11505:3784:12960/150‑1 (MQ=60)
                                                                                                                                 GTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACTCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCA                        <  NB501016:230:HFVJLAFXY:3:11501:11284:1494/150‑1 (MQ=60)
                                                                                                                                  TAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGT                      >  NB501016:230:HFVJLAFXY:3:11510:6202:13116/1‑151 (MQ=60)
                                                                                                                                    GGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGC                    >  NB501016:230:HFVJLAFXY:3:11501:3162:13505/1‑151 (MQ=60)
                                                                                                                                    GGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTC                             >  NB501016:230:HFVJLAFXY:3:11611:17488:1923/1‑142 (MQ=60)
                                                                                                                                       CGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAG                  <  NB501016:230:HFVJLAFXY:3:21412:21166:12849/150‑1 (MQ=60)
                                                                                                                                        GGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCG                >  NB501016:230:HFVJLAFXY:1:11303:10530:8866/1‑151 (MQ=60)
                                                                                                                                        GGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCG                >  NB501016:230:HFVJLAFXY:4:21502:20460:3051/1‑151 (MQ=60)
                                                                                                                                        GGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCG                >  NB501016:230:HFVJLAFXY:4:11507:6777:14455/1‑151 (MQ=60)
                                                                                                                                             AGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTT           <  NB501016:230:HFVJLAFXY:2:11302:9682:18396/151‑1 (MQ=60)
                                                                                                                                                 GTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCT       <  NB501016:230:HFVJLAFXY:1:21206:12820:11309/151‑1 (MQ=60)
                                                                                                                                                     acgCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGT    <  NB501016:230:HFVJLAFXY:3:11410:20070:6110/147‑1 (MQ=60)
                                                                                                                                                      cgCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTG  >  NB501016:230:HFVJLAFXY:3:21610:11180:5367/3‑151 (MQ=60)
                                                                                                                                                      cgCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTC  >  NB501016:230:HFVJLAFXY:2:21209:13010:9858/3‑151 (MQ=60)
                                                                                                                                                        |                                                                                                                                                    
GATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTC  >  NC_000913/4296231‑4296529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑