Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
46.7 |
5280542 |
98.1% |
5180211 |
47.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_017635 |
2,331,461 |
(T)8→7 |
intergenic (‑905/+261) |
ECW_RS11680 ← / ← ECW_RS11685 |
AAA family ATPase/replication endonuclease |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_017635 | 2,331,454 | 0 | T | . | 100.0%
| 156.4
/ NA
| 37 | intergenic (‑898/+268) | ECW_RS11680/ECW_RS11685 | AAA family ATPase/replication endonuclease |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (22/15); total (22/15) |
ATTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGTTTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAG > NC_017635/2331407‑2331495
|
aTTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGggtggt > 1:2197206/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCCGGGTGGttt > 2:2060948/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:1767958/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:2515131/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:254148/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:2595293/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:658268/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:971998/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:1031282/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:1071132/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:1148658/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:1194392/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:1230750/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:1360507/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:972668/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:2003588/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:2270413/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:2333137/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:2452561/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:2607602/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:335725/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:33715/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:380818/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:458293/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:934690/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 2:983887/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:2073841/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:1445851/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:1218513/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:1792092/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:243589/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:1985168/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:1145605/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:2074765/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:2184361/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:2242044/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:104280/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttt > 1:2357630/1‑48 (MQ=255)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGGGGgtt > 2:2591385/1‑48 (MQ=39)
tATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGCGGttt > 1:625634/1‑48 (MQ=255)
aTGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttt > 2:821816/1‑48 (MQ=255)
aTGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttt > 2:16329/1‑48 (MQ=255)
tGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttttt < 2:1204450/48‑1 (MQ=255)
tGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGttttt < 1:469742/48‑1 (MQ=255)
tGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttt < 2:1900619/47‑1 (MQ=255)
gCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttt > 1:51344/1‑48 (MQ=255)
gCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttt > 2:417155/1‑48 (MQ=255)
ttAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGtttttttgc > 2:2257473/1‑46 (MQ=255)
atatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc > 1:824643/1‑48 (MQ=38)
tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGccc < 2:616261/48‑1 (MQ=38)
tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc > 2:918066/1‑47 (MQ=38)
tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc > 2:901889/1‑47 (MQ=38)
tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc > 2:2445751/1‑47 (MQ=38)
tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc > 1:1086262/1‑47 (MQ=38)
tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc > 2:439319/1‑47 (MQ=38)
tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc > 2:625742/1‑47 (MQ=38)
tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc > 1:2045679/1‑47 (MQ=38)
tatGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGcc > 2:501707/1‑47 (MQ=38)
atGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCca > 1:2304466/1‑48 (MQ=38)
atGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCca > 1:1897505/1‑48 (MQ=38)
atGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCca > 1:264169/1‑48 (MQ=38)
atGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCca > 2:1412914/1‑48 (MQ=38)
atGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCca > 1:1620533/1‑48 (MQ=38)
atGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCca > 1:1616234/1‑48 (MQ=38)
atGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCca > 1:575064/1‑48 (MQ=38)
atGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCca > 1:914731/1‑48 (MQ=38)
gCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcat > 2:1822889/1‑47 (MQ=38)
gCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcat > 1:1440836/1‑47 (MQ=38)
gCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcat > 2:1130658/1‑47 (MQ=38)
cTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcatca < 1:2435749/48‑1 (MQ=38)
cTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcatc < 2:231850/47‑1 (MQ=38)
cTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcatc < 1:851571/47‑1 (MQ=38)
cTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcatc < 2:2343589/47‑1 (MQ=38)
tCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcatcat > 1:701212/1‑47 (MQ=38)
caaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCcatcat < 1:2425998/46‑1 (MQ=38)
caaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCatcatc < 2:2201207/47‑1 (MQ=38)
aaacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAtcatca < 2:1089127/47‑1 (MQ=38)
aacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAAt < 1:1200706/48‑1 (MQ=38)
aacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAAt < 2:186227/48‑1 (MQ=38)
aacaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAAt < 1:283571/48‑1 (MQ=38)
acaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATa > 1:1252440/1‑48 (MQ=38)
acaaaAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATa < 2:133645/48‑1 (MQ=38)
accTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGAc < 1:683074/48‑1 (MQ=39)
ccAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGa < 1:1729432/46‑1 (MQ=39)
aGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAg < 2:850370/48‑1 (MQ=39)
|
ATTATGCTTTAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGTTTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAG > NC_017635/2331407‑2331495
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGTTTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAGACTT > NC_017635/2331416‑2331499
|
TAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCC > M01420:84:000000000‑ABVEH:1:1108:11175:4878/1‑51 (MQ=60)
AATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCC > M01420:84:000000000‑ABVEH:1:2110:28760:15103/1‑50 (MQ=60)
AATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCC < M01420:84:000000000‑ABVEH:1:2104:20678:24742/50‑1 (MQ=60)
AATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCC > M01420:84:000000000‑ABVEH:1:2104:20389:14246/1‑50 (MQ=60)
AATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCC > M01420:84:000000000‑ABVEH:1:2110:8318:18335/1‑50 (MQ=60)
AATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCC > M01420:84:000000000‑ABVEH:1:1110:22411:10685/1‑50 (MQ=60)
AATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCC < M01420:84:000000000‑ABVEH:1:2111:22960:17348/50‑1 (MQ=60)
ATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAT > M01420:84:000000000‑ABVEH:1:1108:24903:23343/1‑51 (MQ=60)
ATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAT > M01420:84:000000000‑ABVEH:1:1114:23780:21422/1‑51 (MQ=60)
ATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAT > M01420:84:000000000‑ABVEH:1:1114:24445:20557/1‑51 (MQ=60)
ATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAT > M01420:84:000000000‑ABVEH:1:2106:28137:19623/1‑51 (MQ=60)
ATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAT > M01420:84:000000000‑ABVEH:1:2103:23602:7532/1‑51 (MQ=60)
ATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAT > M01420:84:000000000‑ABVEH:1:1105:12506:26511/1‑51 (MQ=60)
ATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCAT > M01420:84:000000000‑ABVEH:1:1103:19681:10315/1‑51 (MQ=60)
ATGCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATC > M01420:84:000000000‑ABVEH:1:1113:11926:10169/1‑50 (MQ=60)
GCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATC < M01420:84:000000000‑ABVEH:1:2107:26254:22285/51‑1 (MQ=60)
GCTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCAT < M01420:84:000000000‑ABVEH:1:1108:27241:10887/50‑1 (MQ=60)
CTTCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATC > M01420:84:000000000‑ABVEH:1:1107:22165:2939/1‑50 (MQ=60)
TCAAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCA < M01420:84:000000000‑ABVEH:1:2107:22822:20280/49‑1 (MQ=60)
AAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATAC < M01420:84:000000000‑ABVEH:1:1103:28749:14124/51‑1 (MQ=60)
AAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATAC > M01420:84:000000000‑ABVEH:1:1111:25418:19699/1‑51 (MQ=60)
AAACAAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATAC < M01420:84:000000000‑ABVEH:1:1111:18494:9857/51‑1 (MQ=60)
AAAAAAACCACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATG < M01420:84:000000000‑ABVEH:1:2114:6962:15580/51‑1 (MQ=60)
CACCTGCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTG < M01420:84:000000000‑ABVEH:1:1106:20201:24089/51‑1 (MQ=60)
GCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAG < M01420:84:000000000‑ABVEH:1:2114:18785:25662/51‑1 (MQ=60)
GCCAGGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACA < M01420:84:000000000‑ABVEH:1:2101:18356:19741/49‑1 (MQ=60)
GGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAGACTT > M01420:84:000000000‑ABVEH:1:2101:12250:3109/1‑51 (MQ=60)
GGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAGACTT > M01420:84:000000000‑ABVEH:1:1114:20349:13161/1‑51 (MQ=60)
GGGTGG‑TTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAGACTT > M01420:84:000000000‑ABVEH:1:2113:10676:16279/1‑51 (MQ=60)
|
TAATATGCTTCAAACAAAAAAACCACCTGCCAGGGTGGTTTTTTTTGCGCCCATCATCAATACGATGAAAGACTGACAAGACTT > NC_017635/2331416‑2331499
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
