Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,965,001	T→C	M486V (ATG→GTG)	ptsP ←	PtsP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,965,001	0	T	C	99.7%	1156.9 / ‑5.9	368	M486V (ATG→GTG)	ptsP	PtsP
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (191/176); total (191/177)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 385 total aligned reads displayed.

CTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCAT  >  NC_000913/2964911‑2965090

ctTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCAccc                                                                                         <  2:2634111/93‑1 (MQ=255)

 ttCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCAcccc                                                                                        <  2:287064/93‑1 (MQ=255)

  tCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACcccc                                                                                       >  2:4442044/1‑93 (MQ=255)

  tCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACcccc                                                                                       <  1:7149132/93‑1 (MQ=255)

   cGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCt                                                                                      >  2:3587594/1‑93 (MQ=255)

   cGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCt                                                                                      <  1:5311470/93‑1 (MQ=255)

    gCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTg                                                                                     >  1:2175665/1‑93 (MQ=255)

     cTGATCGGCATGTAAGGCATCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGATCATTTGCAGCACCCCCTGa                                                                                    >  1:7033933/1‑93 (MQ=255)

      tGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGAt                                                                                   >  1:4753904/1‑93 (MQ=255)

       gATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATa                                                                                  >  2:4741834/1‑93 (MQ=255)

       gATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATa                                                                                  >  2:7638518/1‑93 (MQ=255)

        aTCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATAc                                                                                 <  2:5357434/93‑1 (MQ=255)

         tCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACt                                                                                >  1:45347/1‑93 (MQ=255)

         tCGGCATGGAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACt                                                                                <  1:374261/93‑1 (MQ=255)

          cGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTg                                                                               <  2:2272263/93‑1 (MQ=255)

           ggCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTgc                                                                              <  2:4535014/93‑1 (MQ=255)

            gCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTgcg                                                                             >  2:7883533/1‑93 (MQ=255)

            gCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTgcg                                                                             >  1:177592/1‑93 (MQ=255)

             cATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGCTACTgcgc                                                                            >  2:1585391/1‑93 (MQ=255)

               tGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCa                                                                          >  2:4192319/1‑93 (MQ=255)

               tGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCa                                                                          >  2:3344548/1‑93 (MQ=255)

               tGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCa                                                                          >  2:6494700/1‑93 (MQ=255)

                 tAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCAcc                                                                        >  1:6961592/1‑93 (MQ=255)

                   aGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTg                                                                      <  2:7336277/93‑1 (MQ=255)

                   aGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTg                                                                      >  2:5453184/1‑93 (MQ=255)

                   aGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTg                                                                      <  1:7749936/93‑1 (MQ=255)

                    ggCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGt                                                                     <  2:1968335/93‑1 (MQ=255)

                    ggCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGt                                                                     >  1:6664815/1‑93 (MQ=255)

                      cAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttc                                                                   <  1:1261774/93‑1 (MQ=255)

                      cAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttc                                                                   <  2:1663105/93‑1 (MQ=255)

                        gctgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttctt                                                                 >  2:6761736/1‑93 (MQ=255)

                          tgctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttct                                                               >  1:5882273/1‑93 (MQ=255)

                           gctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttctt                                                              >  1:5581939/1‑93 (MQ=255)

                           gctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttctt                                                              >  1:4639632/1‑93 (MQ=255)

                           gctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttctt                                                              >  1:6535690/1‑93 (MQ=255)

                            ctTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGttcttcttc                                                             <  1:1672051/93‑1 (MQ=255)

                             ttATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTcc                                                            <  2:5580746/93‑1 (MQ=255)

                             ttATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTcc                                                            <  1:5185161/93‑1 (MQ=255)

                             ttATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTcc                                                            >  1:855178/1‑93 (MQ=255)

                                tCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGAc                                                         >  1:2961056/1‑93 (MQ=255)

                                tCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGAc                                                         >  2:5215149/1‑93 (MQ=255)

                                tCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCACTGCTACTGCGCCACCTGTTCTTCTTCCgcc                                                         >  2:2117412/1‑91 (MQ=255)

                                 cTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACg                                                        <  2:712843/93‑1 (MQ=255)

                                 cTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACg                                                        >  2:6396265/1‑93 (MQ=255)

                                   gCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACggg                                                      >  2:5682963/1‑93 (MQ=255)

                                     tCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGaa                                                    >  1:2286412/1‑93 (MQ=255)

                                     tCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCCACGGGaa                                                    >  1:2680759/1‑93 (MQ=255)

                                       cGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAc                                                  <  1:2058664/93‑1 (MQ=255)

                                        gACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc                                                 <  2:3493025/93‑1 (MQ=255)

                                        gACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc                                                 >  1:360898/1‑93 (MQ=255)

                                        gACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAcc                                                 >  2:2443118/1‑93 (MQ=255)

                                         gcATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCa                                                <  2:7761239/92‑1 (MQ=255)

                                           aTCCAGCGTACGCAGGGTGACGGGTTTATCAGTGAACATTTGCAGCACCCCCTGATTCTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACt                                              <  2:692902/93‑1 (MQ=255)

                                           aTCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACt                                              >  2:4111213/1‑93 (MQ=255)

                                            tCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACtt                                             >  1:2450719/1‑93 (MQ=255)

                                            tCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACtt                                             >  2:178313/1‑93 (MQ=255)

                                            tCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACtt                                             >  1:42174/1‑93 (MQ=255)

                                              cAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTg                                           <  1:6246029/93‑1 (MQ=255)

                                              cAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTg                                           >  2:6315417/1‑93 (MQ=255)

                                                  gTACGCGAGGTGACGGGTTTATCATTGAACATTTGCAGCCCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcagc                                       <  1:5815304/93‑1 (MQ=255)

                                                  gTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcagc                                       >  2:4582815/1‑93 (MQ=255)

                                                  gTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTgcagc                                       >  2:2151946/1‑93 (MQ=255)

                                                    aCGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCAt                                     <  2:324945/93‑1 (MQ=255)

                                                    aCGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCAt                                     >  2:4518250/1‑93 (MQ=255)

                                                     cGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATg                                    <  1:2914843/93‑1 (MQ=255)

                                                      gCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGa                                   >  1:1384243/1‑93 (MQ=255)

                                                       cAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGaa                                  <  2:3847279/93‑1 (MQ=255)

                                                        aaGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAt                                 <  1:2388087/93‑1 (MQ=255)

                                                        aaGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAt                                 <  2:5953595/93‑1 (MQ=255)

                                                         aGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATg                                <  1:1562909/93‑1 (MQ=255)

                                                          ggTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATgg                               <  2:6140491/93‑1 (MQ=255)

                                                            tGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGa                             >  1:7586297/1‑93 (MQ=255)

                                                             gACGGTTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt                            <  2:52582/93‑1 (MQ=255)

                                                             gACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGACTGGGAt                            >  2:426555/1‑93 (MQ=255)

                                                             gACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAt                            >  1:1821675/1‑93 (MQ=255)

                                                               cGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAttt                          >  1:7439846/1‑93 (MQ=255)

                                                                gggTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTc                         <  1:1035434/93‑1 (MQ=255)

                                                                 ggTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCa                        >  2:1995869/1‑93 (MQ=255)

                                                                   tttATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGt                      >  1:3944704/1‑93 (MQ=255)

                                                                      aTCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCg                   >  2:1182519/1‑93 (MQ=255)

                                                                       tCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGa                  <  1:3485806/93‑1 (MQ=255)

                                                                       tCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGa                  >  2:3046633/1‑93 (MQ=255)

                                                                       tCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGa                  <  2:5318226/93‑1 (MQ=255)

                                                                        cATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGAt                 >  2:1396173/1‑93 (MQ=255)

                                                                        cATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGAt                 >  1:6497211/1‑93 (MQ=255)

                                                                        cATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGAt                 <  1:326713/93‑1 (MQ=255)

                                                                         aTTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGTGATTTCAGTGCGATa                >  2:6502321/1‑93 (MQ=255)

                                                                          ttGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATaa               >  2:1574517/1‑93 (MQ=255)

                                                                          ttGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATaa               >  1:7814067/1‑93 (MQ=255)

                                                                            gAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAg             <  1:5444123/93‑1 (MQ=255)

                                                                              aCATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAc           <  2:107614/93‑1 (MQ=255)

                                                                                 tttGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGa        >  2:5088446/1‑93 (MQ=255)

                                                                                 tttGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGa        >  2:6636937/1‑93 (MQ=255)

                                                                                 tttGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGa        <  1:5683449/93‑1 (MQ=255)

                                                                                 tttGCAGCACCCCCTGATACTGCGCCACCGGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGa        <  1:5836864/93‑1 (MQ=255)

                                                                                  ttGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAt       <  2:5390895/93‑1 (MQ=255)

                                                                                    gcagcaCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGc     <  1:213287/93‑1 (MQ=255)

                                                                                    gcagcaCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGc     >  1:1907739/1‑93 (MQ=255)

                                                                                      agcaCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCa   >  1:4968173/1‑93 (MQ=255)

                                                                                      agcaCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCa   <  1:1058472/93‑1 (MQ=255)

CTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCAT  >  NC_000913/2964911‑2965090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 203 total aligned reads displayed.

TCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  NC_000913/2964908‑2965094

TCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCAC                                                                                               >  SRR4302543.7413460/1‑94 (MQ=60)

TCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCAC                                                                                               <  SRR4302543.3654965/94‑1 (MQ=60)

TCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCAC                                                                                               <  SRR4302543.3842065/94‑1 (MQ=60)

 CTCTTCGCTGATCGGCATGTAAGGCCGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGCACCTTTGCAGCACC                                                                                              >  SRR4302543.6048359/1‑94 (MQ=60)

 CTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACC                                                                                              >  SRR4302543.4097327/1‑94 (MQ=60)

 CTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACC                                                                                              >  SRR4302543.7939148/1‑94 (MQ=60)

  TCTTCGCTGATCTGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCC                                                                                             <  SRR4302543.4201231/94‑1 (MQ=60)

   CTTCGCTGATCGGCATGTAAGGCAGCTGCTTATATACTCCGACATCAAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCC                                                                                            >  SRR4302543.2243651/1‑94 (MQ=60)

    TTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCC                                                                                           <  SRR4302543.3581233/94‑1 (MQ=60)

     TCGCTGATCTGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATAATTGAACATTTGCAGCACCCCCT                                                                                          <  SRR4302543.1355371/94‑1 (MQ=60)

     TCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCT                                                                                          <  SRR4302543.5258909/94‑1 (MQ=60)

     TCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCT                                                                                          <  SRR4302543.7218621/94‑1 (MQ=60)

      CGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTG                                                                                         <  SRR4302543.5359446/94‑1 (MQ=60)

      CGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTG                                                                                         <  SRR4302543.4418476/94‑1 (MQ=60)

      CGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTG                                                                                         >  SRR4302543.2195160/1‑94 (MQ=60)

      CGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTG                                                                                         <  SRR4302543.2401593/94‑1 (MQ=60)

       GCTGATCGGCATGTAAGGCATCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGATCATTTGCAGCACCCCCTGA                                                                                        >  SRR4302543.7101613/1‑94 (MQ=60)

        CTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGAT                                                                                       <  SRR4302543.1537843/94‑1 (MQ=60)

        CTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGAT                                                                                       >  SRR4302543.4797350/1‑94 (MQ=60)

         TGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATA                                                                                      <  SRR4302543.186619/94‑1 (MQ=60)

          GATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATAC                                                                                     <  SRR4302543.6640682/94‑1 (MQ=60)

           ATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACT                                                                                    >  SRR4302543.45760/1‑94 (MQ=60)

           ATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACT                                                                                    <  SRR4302543.1901032/94‑1 (MQ=60)

           ATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACT                                                                                    >  SRR4302543.1829979/1‑94 (MQ=60)

           ATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACT                                                                                    <  SRR4302543.1818034/94‑1 (MQ=60)

            TCGGCATGGAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTG                                                                                   <  SRR4302543.377706/94‑1 (MQ=60)

             CGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGC                                                                                  >  SRR4302543.5707218/1‑94 (MQ=60)

             CGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGC                                                                                  >  SRR4302543.5464199/1‑94 (MQ=60)

             CGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGC                                                                                  >  SRR4302543.5882678/1‑94 (MQ=60)

             CGGCATGTAAGGCAGCGTCTTATCTGCGCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCTCCCCCTGATACTGC                                                                                  <  SRR4302543.2550469/94‑1 (MQ=60)

              GGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGctcgtgcg                                                                                 >  SRR4302543.179107/1‑86 (MQ=60)

              GGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCG                                                                                 <  SRR4302543.7590259/94‑1 (MQ=60)

              GGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCG                                                                                 >  SRR4302543.179108/1‑94 (MQ=60)

                CATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCC                                                                               >  SRR4302543.7506054/1‑94 (MQ=60)

                 ATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCA                                                                              <  SRR4302543.5381482/94‑1 (MQ=60)

                 ATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCA                                                                              >  SRR4302543.5774409/1‑94 (MQ=60)

                 ATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCA                                                                              >  SRR4302543.2532716/1‑94 (MQ=60)

                 ATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCA                                                                              >  SRR4302543.1299501/1‑94 (MQ=60)

                  TGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCAC                                                                             >  SRR4302543.2904339/1‑94 (MQ=60)

                   GTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACC                                                                            >  SRR4302543.7027095/1‑94 (MQ=60)

                   GTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACC                                                                            >  SRR4302543.3708960/1‑94 (MQ=60)

                    TAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCT                                                                           >  SRR4302543.7319873/1‑94 (MQ=60)

                    TAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCT                                                                           >  SRR4302543.36397/1‑94 (MQ=60)

                    TAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATATGCAGCACCCCCTGATACTGCGCCACCT                                                                           >  SRR4302543.1914716/1‑94 (MQ=60)

                     AAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTG                                                                          <  SRR4302543.4795429/94‑1 (MQ=60)

                      AGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGT                                                                         <  SRR4302543.7823121/94‑1 (MQ=60)

                      AGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGT                                                                         >  SRR4302543.6727739/1‑94 (MQ=60)

                       GGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTT                                                                        <  SRR4302543.3427206/94‑1 (MQ=60)

                       GGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTT                                                                        <  SRR4302543.930350/94‑1 (MQ=60)

                       GGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTT                                                                        <  SRR4302543.7829068/94‑1 (MQ=60)

                        GCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTC                                                                       <  SRR4302543.7574336/94‑1 (MQ=60)

                        GCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTC                                                                       >  SRR4302543.6068244/1‑94 (MQ=60)

                         CAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCT                                                                      <  SRR4302543.1273069/94‑1 (MQ=60)

                         CAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCT                                                                      <  SRR4302543.3323834/94‑1 (MQ=60)

                         CAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCT                                                                      >  SRR4302543.2944090/1‑94 (MQ=60)

                         CAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCT                                                                      <  SRR4302543.6463069/94‑1 (MQ=60)

                          AGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTT                                                                     <  SRR4302543.2462552/94‑1 (MQ=60)

                            CTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCT                                                                   >  SRR4302543.5935883/1‑94 (MQ=60)

                             TGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTT                                                                  >  SRR4302543.6251226/1‑94 (MQ=60)

                             TGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTT                                                                  >  SRR4302543.4681826/1‑94 (MQ=60)

                             TGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTT                                                                  <  SRR4302543.1353117/94‑1 (MQ=60)

                             TGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTT                                                                  >  SRR4302543.6597211/1‑94 (MQ=60)

                             TGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTT                                                                  >  SRR4302543.6597210/1‑94 (MQ=60)

                             TGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTT                                                                  >  SRR4302543.5632762/1‑94 (MQ=60)

                             TGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTT                                                                  >  SRR4302543.6597209/1‑94 (MQ=60)

                               CTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCC                                                                <  SRR4302543.1687037/94‑1 (MQ=60)

                               CTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCC                                                                >  SRR4302543.862786/1‑94 (MQ=60)

                               CTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCC                                                                <  SRR4302543.1703305/94‑1 (MQ=60)

                               CTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACACCCTGATACTGCGCCACCTGTTCTTCTTCC                                                                >  SRR4302543.4124054/1‑94 (MQ=60)

                                TTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCG                                                               <  SRR4302543.166970/94‑1 (MQ=60)

                                TTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCG                                                               <  SRR4302543.3216185/94‑1 (MQ=60)

                                TTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCG                                                               <  SRR4302543.1953739/94‑1 (MQ=60)

                                TTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCG                                                               <  SRR4302543.5231943/94‑1 (MQ=60)

                                  ATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGAC                                                             >  SRR4302543.2987939/1‑94 (MQ=60)

                                   TCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACG                                                            <  SRR4302543.7874535/94‑1 (MQ=60)

                                   TCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACG                                                            >  SRR4302543.1780019/1‑94 (MQ=60)

                                    CTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGG                                                           <  SRR4302543.7965971/94‑1 (MQ=60)

                                     TGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGG                                                          <  SRR4302543.2194434/94‑1 (MQ=60)

                                       CTCCGGCATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAA                                                        >  SRR4302543.6648731/1‑94 (MQ=60)

                                       CTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAA                                                        >  SRR4302543.2307290/1‑94 (MQ=60)

                                       CTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCCACGGGAA                                                        >  SRR4302543.2705055/1‑94 (MQ=60)

                                         CCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAAC                                                      <  SRR4302543.4148394/94‑1 (MQ=60)

                                          CGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACC                                                     >  SRR4302543.364233/1‑94 (MQ=60)

                                          CGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACC                                                     <  SRR4302543.2077086/94‑1 (MQ=60)

                                           GACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCA                                                    <  SRR4302543.5612887/94‑1 (MQ=60)

                                           GACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCA                                                    >  SRR4302543.5896138/1‑94 (MQ=60)

                                           GACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGAACCCCCTGATACTGCGCCACCTGTTCTTCTTCGGACGGGAAACCA                                                    >  SRR4302543.5896139/1‑94 (MQ=60)

                                            ACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCAC                                                   <  SRR4302543.703091/94‑1 (MQ=60)

                                            ACAGCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCCGTTCTTCTTCCGACGGGAAACCAC                                                   <  SRR4302543.5920742/94‑1 (MQ=60)

                                             CATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACT                                                  <  SRR4302543.5789681/94‑1 (MQ=60)

                                              ATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTT                                                 >  SRR4302543.42560/1‑94 (MQ=60)

                                              ATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTT                                                 <  SRR4302543.4487351/94‑1 (MQ=60)

                                              ATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTT                                                 >  SRR4302543.2472826/1‑94 (MQ=60)

                                              ATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTT                                                 >  SRR4302543.1780528/1‑94 (MQ=60)

                                              ATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTT                                                 >  SRR4302543.3659662/1‑94 (MQ=60)

                                              ATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTT                                                 >  SRR4302543.2451692/1‑94 (MQ=60)

                                              ATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTT                                                 >  SRR4302543.1076711/1‑94 (MQ=60)

                                                ccagggtgcgcaaggtgctaGGTTTATGATTGAACATTTGCCGCCCCCCCTCATACTGCGCCACCTGTTCTTCTGCCGACGGGAAACCGCTGTG                                               <  SRR4302543.5575153/74‑1 (MQ=37)

                                                CCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCGCTTTG                                               <  SRR4302543.5575152/94‑1 (MQ=60)

                                                CCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTG                                               >  SRR4302543.579010/1‑94 (MQ=60)

                                                CCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTG                                               <  SRR4302543.1059036/94‑1 (MQ=60)

                                                CCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTG                                               <  SRR4302543.4882127/94‑1 (MQ=60)

                                                 CAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGC                                              <  SRR4302543.6304955/94‑1 (MQ=60)

                                                   GCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCCCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAG                                            <  SRR4302543.2179096/94‑1 (MQ=60)

                                                   GCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAG                                            <  SRR4302543.4637849/94‑1 (MQ=60)

                                                   GCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAG                                            <  SRR4302543.2957652/94‑1 (MQ=60)

                                                   GCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAG                                            <  SRR4302543.526565/94‑1 (MQ=60)

                                                     GTACGCGAGGTGACGGGTTTATCATTGAACATTTGCAGCCCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCA                                          <  SRR4302543.5868185/94‑1 (MQ=60)

                                                     GTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCA                                          <  SRR4302543.2316238/94‑1 (MQ=60)

                                                     GTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCA                                          >  SRR4302543.909407/1‑94 (MQ=60)

                                                     GTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCA                                          >  SRR4302543.4748122/1‑94 (MQ=60)

                                                      TACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCAT                                         <  SRR4302543.2310069/94‑1 (MQ=60)

                                                      TACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCAT                                         <  SRR4302543.3851574/94‑1 (MQ=60)

                                                      TACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCAT                                         >  SRR4302543.5712079/1‑94 (MQ=60)

                                                       ACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATG                                        <  SRR4302543.1241012/94‑1 (MQ=60)

                                                        CGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGA                                       <  SRR4302543.2941328/94‑1 (MQ=60)

                                                        CGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGA                                       >  SRR4302543.5049746/1‑94 (MQ=60)

                                                        CGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGA                                       >  SRR4302543.1396702/1‑94 (MQ=60)

                                                         GCAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAA                                      >  SRR4302543.2745908/1‑94 (MQ=60)

                                                          CAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAT                                     >  SRR4302543.6956235/1‑94 (MQ=60)

                                                          CAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAT                                     <  SRR4302543.2209344/94‑1 (MQ=60)

                                                          CAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAT                                     >  SRR4302543.3807438/1‑94 (MQ=60)

                                                          CAAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAAT                                     >  SRR4302543.6644166/1‑94 (MQ=60)

                                                           AAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATG                                    <  SRR4302543.5081851/94‑1 (MQ=60)

                                                           AAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATG                                    >  SRR4302543.5011719/1‑94 (MQ=60)

                                                           AAGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATG                                    <  SRR4302543.2409705/94‑1 (MQ=60)

                                                            AGGTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGG                                   <  SRR4302543.1576865/94‑1 (MQ=60)

                                                              GTGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGA                                 >  SRR4302543.7658388/1‑94 (MQ=60)

                                                               TGACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGAT                                >  SRR4302543.1838019/1‑94 (MQ=60)

                                                                 ACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTT                              <  SRR4302543.3081976/94‑1 (MQ=60)

                                                                 ACGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTT                              >  SRR4302543.7511156/1‑94 (MQ=60)

                                                                  CGGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTC                             >  SRR4302543.517492/1‑94 (MQ=60)

                                                                   GGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCA                            >  SRR4302543.4297561/1‑94 (MQ=60)

                                                                   GGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCA                            <  SRR4302543.7735007/94‑1 (MQ=60)

                                                                   GGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCA                            >  SRR4302543.1190768/1‑94 (MQ=60)

                                                                   GGGTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCA                            <  SRR4302543.1044631/94‑1 (MQ=60)

                                                                     GTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGT                          <  SRR4302543.7119409/94‑1 (MQ=60)

                                                                     GTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGT                          <  SRR4302543.7119410/94‑1 (MQ=60)

                                                                     GTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGT                          >  SRR4302543.3979583/1‑94 (MQ=60)

                                                                     GTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGT                          >  SRR4302543.3339852/1‑94 (MQ=60)

                                                                     GTTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGT                          >  SRR4302543.2838934/1‑94 (MQ=60)

                                                                      TTTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTG                         <  SRR4302543.1045461/94‑1 (MQ=60)

                                                                       TTATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGC                        >  SRR4302543.1446629/1‑94 (MQ=60)

                                                                        TATCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCG                       <  SRR4302543.696388/94‑1 (MQ=60)

                                                                          TCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGAT                     <  SRR4302543.3517018/94‑1 (MQ=60)

                                                                          TCATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGAT                     >  SRR4302543.6558419/1‑94 (MQ=60)

                                                                           CATTGAGCATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATA                    <  SRR4302543.7010863/94‑1 (MQ=60)

                                                                           CATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATA                    <  SRR4302543.329676/94‑1 (MQ=60)

                                                                           CATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATA                    >  SRR4302543.7872882/1‑94 (MQ=60)

                                                                           CATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATA                    >  SRR4302543.2150784/1‑94 (MQ=60)

                                                                           CATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATA                    >  SRR4302543.2150785/1‑94 (MQ=60)

                                                                            ATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAA                   >  SRR4302543.7887706/1‑94 (MQ=60)

                                                                            ATTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAA                   >  SRR4302543.5940286/1‑94 (MQ=60)

                                                                             TTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAA                  <  SRR4302543.5515065/94‑1 (MQ=60)

                                                                             TTGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAA                  <  SRR4302543.3619276/94‑1 (MQ=60)

                                                                              TGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAG                 >  SRR4302543.7168916/1‑94 (MQ=60)

                                                                              TGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAG                 <  SRR4302543.5489302/94‑1 (MQ=60)

                                                                              TGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAG                 >  SRR4302543.7342325/1‑94 (MQ=60)

                                                                              TGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAG                 >  SRR4302543.2013850/1‑94 (MQ=60)

                                                                              TGAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAG                 <  SRR4302543.5307543/94‑1 (MQ=60)

                                                                               GAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGA                <  SRR4302543.7990542/94‑1 (MQ=60)

                                                                               GAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGA                >  SRR4302543.4605586/1‑94 (MQ=60)

                                                                               GAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGA                <  SRR4302543.5493534/94‑1 (MQ=60)

                                                                               GAACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGA                <  SRR4302543.4681824/94‑1 (MQ=60)

                                                                               GAACATTTGCAGCACCCCCTGATACTGCGACACCTGTTCTACAACCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGA                <  SRR4302543.7096733/94‑1 (MQ=60)

                                                                                AACATTTGCAGCCCCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAC               <  SRR4302543.7777107/94‑1 (MQ=60)

                                                                                AACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAC               <  SRR4302543.1587728/94‑1 (MQ=60)

                                                                                AACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGAC               >  SRR4302543.4760918/1‑94 (MQ=60)

                                                                                 ACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACC              <  SRR4302543.1961670/94‑1 (MQ=60)

                                                                                 ACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACC              >  SRR4302543.1561516/1‑94 (MQ=60)

                                                                                 ACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACC              >  SRR4302543.6571877/1‑94 (MQ=60)

                                                                                 ACATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACC              <  SRR4302543.3949837/94‑1 (MQ=60)

                                                                                  CATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCG             >  SRR4302543.1580451/1‑94 (MQ=60)

                                                                                  CATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCG             >  SRR4302543.6508901/1‑94 (MQ=60)

                                                                                  CATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCG             <  SRR4302543.2166524/94‑1 (MQ=60)

                                                                                   ATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGA            >  SRR4302543.2622420/1‑94 (MQ=60)

                                                                                   ATTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGA            >  SRR4302543.6545109/1‑94 (MQ=60)

                                                                                    TTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAT           <  SRR4302543.5879614/94‑1 (MQ=60)

                                                                                    TTTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAT           <  SRR4302543.2289506/94‑1 (MQ=60)

                                                                                    TTTGCAGCACCCCCTGATACTGCGCCACCGGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGAT           <  SRR4302543.5889954/94‑1 (MQ=60)

                                                                                     TTGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATG          <  SRR4302543.7547011/94‑1 (MQ=60)

                                                                                      TGCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGC         >  SRR4302543.1924921/1‑94 (MQ=60)

                                                                                       GCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCC        <  SRR4302543.215109/94‑1 (MQ=60)

                                                                                       GCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCC        <  SRR4302543.215110/94‑1 (MQ=60)

                                                                                       GCAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCC        >  SRR4302543.5714747/1‑94 (MQ=60)

                                                                                        CAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCA       >  SRR4302543.5013393/1‑94 (MQ=60)

                                                                                        CAGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCA       <  SRR4302543.4255357/94‑1 (MQ=60)

                                                                                         AGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCAT      <  SRR4302543.1067963/94‑1 (MQ=60)

                                                                                         AGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCAT      >  SRR4302543.3196090/1‑94 (MQ=60)

                                                                                         AGCACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCAT      >  SRR4302543.7785924/1‑94 (MQ=60)

                                                                                           CACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCA    >  SRR4302543.7308341/1‑94 (MQ=60)

                                                                                            ACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAA   >  SRR4302543.3862672/1‑94 (MQ=60)

                                                                                            ACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAA   >  SRR4302543.4550641/1‑94 (MQ=60)

                                                                                            ACCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAA   >  SRR4302543.3862673/1‑94 (MQ=60)

                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  SRR4302543.7420795/1‑94 (MQ=60)

                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  <  SRR4302543.2540710/94‑1 (MQ=60)

                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  SRR4302543.7467809/1‑94 (MQ=60)

                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  <  SRR4302543.646858/94‑1 (MQ=60)

                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  SRR4302543.4090739/1‑94 (MQ=60)

                                                                                             CCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  SRR4302543.2487048/1‑94 (MQ=60)

TCTCTTCGCTGATCGGCATGTAAGGCAGCTGCTTATCTGCTCCGACATCCAGCGTACGCAAGGTGACGGGTTTATCATTGAACATTTGCAGCATCCCCTGATACTGCGCCACCTGTTCTTCTTCCGACGGGAAACCACTTTGCAGCATGAATGGGATTTCAGTGCGATAAAGACCGATGCCATCAAT  >  NC_000913/2964908‑2965094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 6 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`