Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,640	+G	intergenic (‑291/+23)	cmtB ← / ← tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,640	1	.	G	99.5%	693.3 / ‑4.0	219	intergenic (‑291/+23)	cmtB/tktA	PTS system mannitol‑specific enzyme IIA component/transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (163/55); total (163/56)
Fisher's exact test for biased strand distribution p-value = 2.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGC  >  NC_000913/3077548‑3077726

tattGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGc                                                                                         >  2:2960743/1‑93 (MQ=255)

tattGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGc                                                                                         <  2:1232792/93‑1 (MQ=255)

 attGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcg                                                                                        >  1:6038893/1‑92 (MQ=255)

  ttGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcga                                                                                       >  2:7182337/1‑91 (MQ=255)

  ttGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcga                                                                                       >  1:507674/1‑91 (MQ=255)

   tGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcgac                                                                                      >  2:3761873/1‑90 (MQ=255)

    gCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcgacc                                                                                     >  1:230248/1‑89 (MQ=255)

    gCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGcgacc                                                                                     >  2:4214232/1‑89 (MQ=255)

      atatAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtt                                                                                   >  1:4657416/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttt                                                                                  >  2:7764813/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttt                                                                                  >  1:5189387/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttt                                                                                  >  1:5096750/1‑93 (MQ=255)

       tataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttt                                                                                  >  2:7062037/1‑93 (MQ=255)

        ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                 >  2:4076835/1‑93 (MQ=255)

        ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                 >  2:3785780/1‑93 (MQ=255)

        ataAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                                                                                 >  2:6191751/1‑93 (MQ=255)

         taAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttttt                                                                                >  1:268050/1‑93 (MQ=255)

          aaaTATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTa                                                                               >  2:2865897/1‑93 (MQ=255)

            atatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAcc                                                                             >  2:6558268/1‑93 (MQ=255)

             tatTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAccc                                                                            >  2:3519291/1‑93 (MQ=255)

              atTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCg                                                                           >  2:4109947/1‑93 (MQ=255)

               tttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAATCGACCTTTTTACCCGa                                                                          >  2:1362671/1‑93 (MQ=255)

               tttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGa                                                                          >  2:4982252/1‑93 (MQ=255)

               tttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGa                                                                          >  1:2503606/1‑93 (MQ=255)

               tttGTGATCTACAACACGCCTTACCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGa                                                                          >  2:5644094/1‑93 (MQ=255)

                ttGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGaa                                                                         >  1:6252550/1‑93 (MQ=255)

                 tgtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGaaa                                                                        <  1:6884227/93‑1 (MQ=255)

                  gtgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAt                                                                       >  1:7748920/1‑93 (MQ=255)

                   tgATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATg                                                                      >  1:4913310/1‑93 (MQ=255)

                    gATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGc                                                                     >  2:3090624/1‑93 (MQ=255)

                     aTCTACAACACGCCTTATCTATTGCTTGTCCGCGAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCt                                                                    >  1:5382448/1‑93 (MQ=255)

                      tCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTa                                                                   <  1:6731475/93‑1 (MQ=255)

                       cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTaa                                                                  <  1:4290349/93‑1 (MQ=255)

                       cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTaa                                                                  >  2:1626134/1‑93 (MQ=255)

                       cTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTaa                                                                  <  1:3452072/93‑1 (MQ=255)

                         acaacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAtt                                                                >  1:3174236/1‑93 (MQ=255)

                          caacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTa                                                               >  2:4637251/1‑93 (MQ=255)

                           aacaCGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAc                                                              >  2:6073378/1‑93 (MQ=255)

                              acGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcagc                                                           >  2:2362380/1‑93 (MQ=255)

                               cGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcagca                                                          >  2:7102125/1‑93 (MQ=255)

                                gCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcagcag                                                         <  2:3611763/93‑1 (MQ=255)

                                gCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTAcagcag                                                         >  1:7830926/1‑93 (MQ=255)

                                gCCTTATCTATTGCTTGTCCGCAAACCGACCAATCAAGGTAAAAAAATAGCTCGCCGAAGCGACATTTTTACCCGAATTGCCAATTAcagcag                                                         >  2:6773511/1‑93 (MQ=255)

                                  cTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGtt                                                       >  2:3169582/1‑93 (MQ=255)

                                  cTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGtt                                                       >  1:2964915/1‑93 (MQ=255)

                                   ttATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTc                                                      <  1:6994912/93‑1 (MQ=255)

                                   ttATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTc                                                      >  2:2151460/1‑93 (MQ=255)

                                    tATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCt                                                     >  2:7553254/1‑93 (MQ=255)

                                      tCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCttt                                                   >  1:6350424/1‑93 (MQ=255)

                                       cTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCtttt                                                  <  1:286543/93‑1 (MQ=255)

                                       cTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCtttt                                                  <  2:2773086/93‑1 (MQ=255)

                                        tATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTg                                                 >  2:4496/1‑93 (MQ=255)

                                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGc                                                >  2:7598618/1‑93 (MQ=255)

                                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGc                                                <  2:7352883/93‑1 (MQ=255)

                                         aTTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGc                                                <  2:5000688/93‑1 (MQ=255)

                                             cTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTc                                            >  1:4718194/1‑93 (MQ=255)

                                              ttGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCg                                           >  1:3936684/1‑93 (MQ=255)

                                              ttGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCg                                           <  1:3550402/93‑1 (MQ=255)

                                                 tCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaa                                        >  2:1834117/1‑93 (MQ=255)

                                                    gCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGcaacaa                                     >  1:1875048/1‑93 (MQ=255)

                                                       aaCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGt                                  <  1:3208148/93‑1 (MQ=255)

                                                       aaCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGt                                  >  1:3419293/1‑93 (MQ=255)

                                                       aaCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGt                                  <  2:7692958/93‑1 (MQ=255)

                                                        aCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGtt                                 >  2:5851111/1‑93 (MQ=255)

                                                        aCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGtt                                 >  1:176951/1‑93 (MQ=255)

                                                        aCGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGtt                                 >  1:3754308/1‑93 (MQ=255)

                                                          ggACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTAt                               >  1:6940503/1‑93 (MQ=255)

                                                          ggACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTAt                               >  1:910912/1‑93 (MQ=255)

                                                           gACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc                              <  1:6541997/93‑1 (MQ=255)

                                                           gACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc                              >  1:4528205/1‑93 (MQ=255)

                                                           gACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc                              >  1:1043794/1‑93 (MQ=255)

                                                           gACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATc                              <  2:5164055/93‑1 (MQ=255)

                                                             cATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCaa                            >  2:5069947/1‑93 (MQ=255)

                                                              atatCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAc                           >  1:7309639/1‑93 (MQ=255)

                                                              atatCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAc                           <  2:1408926/93‑1 (MQ=255)

                                                               tatCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACa                          >  1:3037415/1‑93 (MQ=255)

                                                                 tCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTGTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGt                        <  1:7642125/93‑1 (MQ=255)

                                                                  cAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTg                       >  1:5592764/1‑93 (MQ=255)

                                                                   aaGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGa                      >  2:3723079/1‑93 (MQ=255)

                                                                   aaGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGa                      >  1:1533851/1‑93 (MQ=255)

                                                                   aaGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGa                      >  2:3381679/1‑93 (MQ=255)

                                                                    aGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa                     >  1:7375412/1‑93 (MQ=255)

                                                                    aGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa                     <  1:3496406/93‑1 (MQ=255)

                                                                    aGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGaa                     >  2:6858451/1‑93 (MQ=255)

                                                                     ggTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAg                    >  2:4730871/1‑93 (MQ=255)

                                                                      gTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGc                   <  1:7070949/93‑1 (MQ=255)

                                                                       taataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGcc                  >  1:3979815/1‑93 (MQ=255)

                                                                        aataaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCg                 >  1:5762841/1‑93 (MQ=255)

                                                                          taaAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGaa               >  1:2082053/1‑93 (MQ=255)

                                                                            aaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAAct             >  2:4826548/1‑93 (MQ=255)

                                                                            aaaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAAct             >  1:53065/1‑93 (MQ=255)

                                                                             aaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAGTTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActc            >  2:2620908/1‑93 (MQ=255)

                                                                             aaaaGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAActc            >  1:5840130/1‑93 (MQ=255)

                                                                                aGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTc         >  1:5936892/1‑93 (MQ=255)

                                                                                  gTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCaa       >  2:5791571/1‑93 (MQ=255)

                                                                                     gCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAca    <  1:2213629/93‑1 (MQ=255)

                                                                                     gCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAca    >  1:616765/1‑93 (MQ=255)

                                                                                     gCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAca    >  2:538742/1‑93 (MQ=255)

                                                                                     gCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAca    >  1:1150917/1‑93 (MQ=255)

                                                                                      ccGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAAcag   >  2:6991436/1‑93 (MQ=255)

TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGC  >  NC_000913/3077548‑3077726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  NC_000913/3077547‑3077733

TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC                                                                                                >  SRR4302543.2721114/1‑94 (MQ=60)

 TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑G                                                                                              >  SRR4302543.6096055/1‑94 (MQ=60)

 TATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGAAATAAAAAAGGTCGCCGAAGC‑G                                                                                              >  SRR4302543.2186097/1‑94 (MQ=60)

  ATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCga                                                                                              >  SRR4302543.512394/1‑92 (MQ=60)

  ATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCga                                                                                              >  SRR4302543.512616/1‑92 (MQ=60)

  ATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCga                                                                                              >  SRR4302543.586669/1‑92 (MQ=60)

    TGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACC                                                                                            >  SRR4302543.232238/1‑94 (MQ=60)

     GCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCT                                                                                           <  SRR4302543.3482159/94‑1 (MQ=60)

      CATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTT                                                                                          >  SRR4302543.4699833/1‑94 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302543.5142905/1‑94 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302543.3499222/1‑94 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302543.5236192/1‑94 (MQ=60)

       ATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTT                                                                                         >  SRR4302543.6925271/1‑94 (MQ=60)

         ATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTT                                                                                       >  SRR4302543.7619538/1‑94 (MQ=60)

         ATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTT                                                                                       >  SRR4302543.270406/1‑94 (MQ=60)

           AAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTAC                                                                                     >  SRR4302543.7010767/1‑94 (MQ=60)

             ATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCC                                                                                   <  SRR4302543.1063045/94‑1 (MQ=60)

             ATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCC                                                                                   >  SRR4302543.3094241/1‑94 (MQ=60)

               ATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGA                                                                                 >  SRR4302543.3214537/1‑94 (MQ=60)

               ATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGA                                                                                 >  SRR4302543.4635720/1‑94 (MQ=60)

               ATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGA                                                                                 >  SRR4302543.2526336/1‑94 (MQ=60)

                TTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAA                                                                                >  SRR4302543.6311528/1‑94 (MQ=60)

                 TTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAA                                                                               >  SRR4302543.2906523/1‑94 (MQ=60)

                  TGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAT                                                                              >  SRR4302543.7822098/1‑94 (MQ=60)

                  TGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAT                                                                              <  SRR4302543.6948989/94‑1 (MQ=60)

                  TGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAAT                                                                              >  SRR4302543.5210717/1‑94 (MQ=60)

                   GTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATG                                                                             >  SRR4302543.4958085/1‑94 (MQ=60)

                     GATCTACAACACGCCTTCTCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCT                                                                           <  SRR4302543.7094005/94‑1 (MQ=60)

                     GATCTACAACACGCCTTATCTATTGCTTGTCCGCGAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCT                                                                           >  SRR4302543.5431300/1‑94 (MQ=60)

                     GATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGACATGCT                                                                           >  SRR4302543.4311141/1‑94 (MQ=60)

                     GATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCT                                                                           >  SRR4302543.2575136/1‑94 (MQ=60)

                      ATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTA                                                                          >  SRR4302543.7383268/1‑94 (MQ=60)

                      ATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTA                                                                          >  SRR4302543.5810989/1‑94 (MQ=60)

                       TCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAA                                                                         <  SRR4302543.6795065/94‑1 (MQ=60)

                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        <  SRR4302543.7531554/94‑1 (MQ=60)

                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        <  SRR4302543.4329355/94‑1 (MQ=60)

                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        <  SRR4302543.3482984/94‑1 (MQ=60)

                        CTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAAT                                                                        <  SRR4302543.6319643/94‑1 (MQ=60)

                         TACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATT                                                                       >  SRR4302543.3202834/1‑94 (MQ=60)

                             ACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAG                                                                   >  SRR4302543.481269/1‑94 (MQ=60)

                             ACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAG                                                                   <  SRR4302543.3581056/94‑1 (MQ=60)

                              CACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGC                                                                  >  SRR4302543.4423720/1‑94 (MQ=60)

                               ACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCA                                                                 <  SRR4302543.5253216/94‑1 (MQ=60)

                               ACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCA                                                                 >  SRR4302543.1756482/1‑94 (MQ=60)

                                CGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAG                                                                >  SRR4302543.7904661/1‑94 (MQ=60)

                                  CCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTT                                                              >  SRR4302543.2991846/1‑94 (MQ=60)

                                  CCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTT                                                              >  SRR4302543.2991847/1‑94 (MQ=60)

                                    TTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCT                                                            <  SRR4302543.6041835/94‑1 (MQ=60)

                                    TTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCT                                                            <  SRR4302543.7060861/94‑1 (MQ=60)

                                     TATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTT                                                           >  SRR4302543.4013781/1‑94 (MQ=60)

                                      ATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTT                                                          >  SRR4302543.6410134/1‑94 (MQ=60)

                                       TCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTT                                                         <  SRR4302543.3987879/94‑1 (MQ=60)

                                       TCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTT                                                         >  SRR4302543.4759030/1‑94 (MQ=60)

                                        CTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTG                                                        >  SRR4302543.5675533/1‑94 (MQ=60)

                                        CTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTG                                                        >  SRR4302543.3058640/1‑94 (MQ=60)

                                        CTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTG                                                        <  SRR4302543.289093/94‑1 (MQ=60)

                                         TATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGC                                                       >  SRR4302543.6381487/1‑94 (MQ=60)

                                         TATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGC                                                       >  SRR4302543.6274730/1‑94 (MQ=60)

                                          ATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCT                                                      >  SRR4302543.1319399/1‑94 (MQ=60)

                                            TGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTT                                                    >  SRR4302543.204721/1‑94 (MQ=60)

                                             GCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTC                                                   <  SRR4302543.5243252/94‑1 (MQ=60)

                                             GCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTC                                                   >  SRR4302543.7892252/1‑94 (MQ=60)

                                             GCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTC                                                   >  SRR4302543.4761307/1‑94 (MQ=60)

                                              CTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCG                                                  >  SRR4302543.3971471/1‑94 (MQ=60)

                                              CTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCG                                                  >  SRR4302543.7870242/1‑94 (MQ=60)

                                              CTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCG                                                  >  SRR4302543.1743750/1‑94 (MQ=60)

                                               TTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGC                                                 <  SRR4302543.3581899/94‑1 (MQ=60)

                                                   CCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACA                                             >  SRR4302543.6720438/1‑94 (MQ=60)

                                                    CGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAA                                            >  SRR4302543.1891893/1‑94 (MQ=60)

                                                    CGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAA                                            >  SRR4302543.3395827/1‑94 (MQ=60)

                                                    CGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTATTTTGCTTTCGCAACAA                                            <  SRR4302543.5642343/94‑1 (MQ=60)

                                                      CAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACG                                          >  SRR4302543.2447453/1‑94 (MQ=60)

                                                       AAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGT                                         >  SRR4302543.3449897/1‑94 (MQ=60)

                                                        AACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTT                                        >  SRR4302543.2291388/1‑94 (MQ=60)

                                                        AACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTT                                        <  SRR4302543.3237102/94‑1 (MQ=60)

                                                        AACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTT                                        >  SRR4302543.178464/1‑94 (MQ=60)

                                                        AACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTT                                        >  SRR4302543.3787339/1‑94 (MQ=60)

                                                          CGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTAT                                      >  SRR4302543.918920/1‑94 (MQ=60)

                                                          CGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTAT                                      >  SRR4302543.7005788/1‑94 (MQ=60)

                                                          CGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTAT                                      <  SRR4302543.3565700/94‑1 (MQ=60)

                                                           GGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATC                                     >  SRR4302543.1053103/1‑94 (MQ=60)

                                                           GGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATC                                     >  SRR4302543.4569418/1‑94 (MQ=60)

                                                           GGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATC                                     >  SRR4302543.4569419/1‑94 (MQ=60)

                                                            GACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCA                                    <  SRR4302543.6603574/94‑1 (MQ=60)

                                                              CATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAC                                  >  SRR4302543.7380108/1‑94 (MQ=60)

                                                              CATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAAC                                  >  SRR4302543.2215897/1‑94 (MQ=60)

                                                               ATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACA                                 <  SRR4302543.6411906/94‑1 (MQ=60)

                                                               ATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACA                                 >  SRR4302543.3064824/1‑94 (MQ=60)

                                                               ATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACA                                 >  SRR4302543.6137439/1‑94 (MQ=60)

                                                                TATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAG                                >  SRR4302543.2616676/1‑94 (MQ=60)

                                                                TATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAG                                <  SRR4302543.1849487/94‑1 (MQ=60)

                                                                  TCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTG                              >  SRR4302543.5643707/1‑94 (MQ=60)

                                                                  TCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTGTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTG                              <  SRR4302543.7714609/94‑1 (MQ=60)

                                                                   CAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGA                             >  SRR4302543.6982130/1‑94 (MQ=60)

                                                                   CAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGA                             >  SRR4302543.1547594/1‑94 (MQ=60)

                                                                    AAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAA                            >  SRR4302543.7446311/1‑94 (MQ=60)

                                                                     AGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAG                           <  SRR4302543.3527686/94‑1 (MQ=60)

                                                                       GTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCC                         >  SRR4302543.6084699/1‑94 (MQ=60)

                                                                       GTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCC                         <  SRR4302543.7139608/94‑1 (MQ=60)

                                                                       GTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCC                         >  SRR4302543.4015107/1‑94 (MQ=60)

                                                                       GTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTACGCAACAACGTTATCAACAGTGAAGCC                         <  SRR4302543.7139607/94‑1 (MQ=60)

                                                                        TAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCG                        <  SRR4302543.7992649/94‑1 (MQ=60)

                                                                        TAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCG                        >  SRR4302543.5815336/1‑94 (MQ=60)

                                                                        TAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCG                        >  SRR4302543.6845853/1‑94 (MQ=60)

                                                                          ATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAA                      >  SRR4302543.2100647/1‑94 (MQ=60)

                                                                          ATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAA                      >  SRR4302543.3675979/1‑94 (MQ=60)

                                                                          ATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAA                      >  SRR4302543.7763299/1‑94 (MQ=60)

                                                                            AAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACT                    <  SRR4302543.3794983/94‑1 (MQ=60)

                                                                            AAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACT                    >  SRR4302543.53535/1‑94 (MQ=60)

                                                                             AAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTC                   >  SRR4302543.5428145/1‑94 (MQ=60)

                                                                             AAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTC                   >  SRR4302543.5893254/1‑94 (MQ=60)

                                                                                AAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTC                <  SRR4302543.5010279/94‑1 (MQ=60)

                                                                                AAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTC                >  SRR4302543.5991200/1‑94 (MQ=60)

                                                                                AAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTC                <  SRR4302543.7343621/94‑1 (MQ=60)

                                                                                AAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTC                <  SRR4302543.5010280/94‑1 (MQ=60)

                                                                                     CGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACA           >  SRR4302543.1161406/1‑94 (MQ=60)

                                                                                     CGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACA           >  SRR4302543.339338/1‑94 (MQ=60)

                                                                                     CGCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACA           >  SRR4302543.622403/1‑94 (MQ=60)

                                                                                      GCCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAG          <  SRR4302543.2233577/94‑1 (MQ=60)

                                                                                       CCGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGC         >  SRR4302543.5976599/1‑94 (MQ=60)

                                                                                        CGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCA        >  SRR4302543.6772105/1‑94 (MQ=60)

                                                                                        CGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCA        >  SRR4302543.3339285/1‑94 (MQ=60)

                                                                                        CGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCA        >  SRR4302543.144074/1‑94 (MQ=60)

                                                                                        CGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCA        >  SRR4302543.1586029/1‑94 (MQ=60)

                                                                                        CGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCA        >  SRR4302543.6400567/1‑94 (MQ=60)

                                                                                        CGAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGACGAACTCTTCAAACAGCA        >  SRR4302543.147892/1‑94 (MQ=60)

                                                                                         GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAG       >  SRR4302543.1883659/1‑94 (MQ=60)

                                                                                         GAAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACACTGAAGCCGAACTCTTCAAACAGCAG       <  SRR4302543.2928461/94‑1 (MQ=60)

                                                                                          AAGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGC      >  SRR4302543.6904544/1‑94 (MQ=60)

                                                                                           AGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCT     >  SRR4302543.634755/1‑94 (MQ=60)

                                                                                           AGCGACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCT     >  SRR4302543.2178242/1‑94 (MQ=60)

                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302543.2192826/1‑94 (MQ=60)

                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302543.5309796/1‑94 (MQ=60)

                                                                                             G‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  SRR4302543.6278985/1‑94 (MQ=60)

TTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGC‑ACCTTTTTACCCGAAATGCTAATTACAGCAGTTCTTTTGCTTTCGCAACAACGTTATCAACAGTGAAGCCGAACTCTTCAAACAGCAGCTCTG  >  NC_000913/3077547‑3077733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`