Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,251,140	G→C	L239V (CTT→GTT)	yhaJ ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,251,140	0	G	C	99.1%	694.9 / ‑1.4	225	coding (715/897 nt)	yhaJ	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (158/64); minor base . (2/0); total (161/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

Only 100 of 238 total aligned reads displayed.

TCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGG  >  NC_000913/3251050‑3251228

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCCCGCCAAccc                                                                                        >  1:2328471/1‑93 (MQ=255)

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAccc                                                                                        >  2:1672040/1‑93 (MQ=255)

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAccc                                                                                        <  1:1913486/93‑1 (MQ=255)

  tcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACcccc                                                                                      >  1:2545934/1‑93 (MQ=255)

    gCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGc                                                                                    >  1:6338542/1‑93 (MQ=255)

     cTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCTCGcc                                                                                   >  1:4707313/1‑93 (MQ=255)

     cTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGcc                                                                                   >  1:5325806/1‑93 (MQ=255)

      tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCa                                                                                  >  1:2733277/1‑93 (MQ=255)

         tCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTa                                                                               <  2:6613604/93‑1 (MQ=255)

           gATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAAt                                                                             <  1:4225274/93‑1 (MQ=255)

           gATTCCGGCCTGACGACACCCAACCCCCCTTCCTCAATATCTTTTTCGACCAAAGGATACGGCATCGTCGCCACCCCAACCCCCGCCAGTAAt                                                                             <  2:5230282/93‑1 (MQ=255)

            aTTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATg                                                                            >  1:3829113/1‑93 (MQ=255)

             ttCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGc                                                                           >  2:555866/1‑93 (MQ=255)

              tCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGcc                                                                          >  1:406884/1‑93 (MQ=255)

                 gggCTGACGACACGCAAGCGCCCTGCCGCAATATATTTTTCGACCATAGGATACGGCATCGTCGCGACGCCAAGCCACGCCAGTAATGCCTGa                                                                       >  2:1925412/1‑93 (MQ=255)

                 gggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGa                                                                       <  2:4410526/93‑1 (MQ=255)

                  ggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATATGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGAc                                                                      >  1:1496264/1‑93 (MQ=255)

                  ggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGAc                                                                      >  2:3108492/1‑93 (MQ=255)

                    cTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGt                                                                    >  1:7927189/1‑93 (MQ=255)

                     tGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGtt                                                                   <  1:3699990/93‑1 (MQ=255)

                      gacgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGttt                                                                  >  2:6292240/1‑93 (MQ=255)

                      gacgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGttt                                                                  >  1:983628/1‑93 (MQ=255)

                      gacgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGttt                                                                  >  2:4968792/1‑93 (MQ=255)

                       acgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTa                                                                 <  2:6957634/93‑1 (MQ=255)

                           cacGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCtt                                                             >  1:5920776/1‑93 (MQ=255)

                            acGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTc                                                            >  2:5755647/1‑93 (MQ=255)

                             cGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCa                                                           <  2:5304474/93‑1 (MQ=255)

                              gaaaCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCac                                                          <  2:7023626/91‑2 (MQ=255)

                               cAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAAt                                                         >  1:6864378/1‑93 (MQ=255)

                               cAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAAt                                                         >  1:4806193/1‑93 (MQ=255)

                                aaCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATc                                                        <  2:1041967/93‑1 (MQ=255)

                                aaCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATc                                                        >  1:2172351/1‑93 (MQ=255)

                                 aCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCg                                                       >  2:2783675/1‑93 (MQ=255)

                                 aCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCg                                                       >  1:2028033/1‑93 (MQ=255)

                                  ccGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGt                                                      >  1:3630131/1‑93 (MQ=255)

                                  ccGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGt                                                      <  2:6860715/93‑1 (MQ=255)

                                  ccGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAA‑CCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTg                                                     >  1:3409573/1‑93 (MQ=255)

                                      ccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTc                                                  <  2:2335712/93‑1 (MQ=255)

                                      ccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTc                                                  >  1:6717463/1‑93 (MQ=255)

                                       cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCa                                                 <  1:7915226/93‑1 (MQ=255)

                                        ttCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAc                                                <  2:5367755/93‑1 (MQ=255)

                                        ttCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAc                                                >  2:7585359/1‑93 (MQ=255)

                                         tCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAcc                                               >  2:374083/1‑93 (MQ=255)

                                            gCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGtt                                            <  1:5929758/93‑1 (MQ=255)

                                             cAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTa                                           >  2:1441072/1‑93 (MQ=255)

                                             cAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTa                                           >  2:2522936/1‑93 (MQ=255)

                                              aaTATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTaa                                          >  1:4599689/1‑93 (MQ=255)

                                              aaTATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTaa                                          <  2:2606842/93‑1 (MQ=255)

                                              aaTATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTaa                                          >  2:5804924/1‑93 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                         >  1:7277151/1‑93 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                         >  1:1167409/1‑93 (MQ=255)

                                                tatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgc                                        >  1:5716888/1‑93 (MQ=255)

                                                tatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgc                                        >  2:453268/1‑93 (MQ=255)

                                                 atCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcg                                       >  2:7446124/1‑93 (MQ=255)

                                                  tCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcgc                                      >  2:2928389/1‑93 (MQ=255)

                                                  tCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcgc                                      >  2:5884634/1‑93 (MQ=255)

                                                  tCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgcgc                                      >  2:1828393/1‑93 (MQ=255)

                                                    tttttCGACCATAGGGTACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCgg                                    >  2:7823831/1‑93 (MQ=255)

                                                    tttttCGACCATAGGATACGGCATCGTCCCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCgg                                    <  1:78259/93‑1 (MQ=255)

                                                     ttttCGACCATATGATTCGGCATCGTCGCCACGCCAATCCCCGCCTGTAATGCCTGACGGTTATCTTCAATCGTGTTCACTGTTAATCGCGGc                                   >  2:7035969/1‑93 (MQ=255)

                                                           aCCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTg                             <  2:2278877/93‑1 (MQ=255)

                                                           aCCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTg                             >  1:1043309/1‑93 (MQ=255)

                                                           aCCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGCGCTCACCGTTAAGCGCGGCTGTTTg                             >  1:4490830/1‑93 (MQ=255)

                                                            ccATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                            >  1:1448190/1‑93 (MQ=255)

                                                             cATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTc                           >  2:3813013/1‑93 (MQ=255)

                                                             cATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTc                           >  2:3558667/1‑93 (MQ=255)

                                                              aTAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTcc                          >  2:6180038/1‑93 (MQ=255)

                                                              aTAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTcc                          >  1:3010465/1‑93 (MQ=255)

                                                              aTAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTcc                          <  1:1971074/93‑1 (MQ=255)

                                                              aTAGGATACGGCATCGTCGCCACGCCAA‑CCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCca                         >  2:4153125/1‑93 (MQ=255)

                                                               tAGGATACGGCATTGTCGTCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCca                         >  2:5487151/1‑93 (MQ=255)

                                                               tAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAGCGTTAAGCGCGGCTGTTTGTCca                         >  1:5815841/1‑93 (MQ=255)

                                                               tAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCca                         >  1:2893478/1‑93 (MQ=255)

                                                                 ggATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagc                       <  1:1097825/93‑1 (MQ=255)

                                                                 ggATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagc                       <  1:2245367/93‑1 (MQ=255)

                                                                 ggATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagc                       >  2:5440543/1‑93 (MQ=255)

                                                                 ggATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagc                       <  1:6574641/93‑1 (MQ=255)

                                                                  gATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagca                      >  1:3495434/1‑93 (MQ=255)

                                                                    tACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCagcagc                    >  1:5024337/1‑93 (MQ=255)

                                                                      cGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTg                  >  2:2256253/1‑93 (MQ=255)

                                                                      cGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTg                  >  1:6955741/1‑93 (MQ=255)

                                                                        gCATCGTCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                <  1:750532/93‑1 (MQ=255)

                                                                        gCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                <  1:7245347/93‑1 (MQ=255)

                                                                        gCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                >  1:4913/1‑93 (MQ=255)

                                                                         cATCGCCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTAc               <  1:2947578/93‑1 (MQ=255)

                                                                          aTCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTTACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACg              >  2:3971538/1‑93 (MQ=255)

                                                                           tcgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACgg             >  1:4398868/1‑93 (MQ=255)

                                                                           tcgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACgg             >  2:1150014/1‑93 (MQ=255)

                                                                           tcgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACgg             >  2:7438454/1‑93 (MQ=255)

                                                                           tcgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACgg             >  1:7744061/1‑93 (MQ=255)

                                                                           tcgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACgg             >  1:1750572/1‑93 (MQ=255)

                                                                            catcgCCACGCCACCCCCCGCCAGTAATGCCTGACGTCTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTATCCAGCAGCTGTACGGt            <  2:7229192/91‑1 (MQ=255)

                                                                              tcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGGTTGTCCAGCAGCTGTCCGGTCa          >  2:131360/1‑93 (MQ=255)

                                                                               cgccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCaa         >  1:5671502/1‑93 (MQ=255)

                                                                                 ccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAaca       <  1:3295992/93‑1 (MQ=255)

                                                                                  cacgccaCCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAacac      <  1:1345332/93‑1 (MQ=255)

                                                                                   acgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGGCCAGCAGCTGTACGGTCAACAcc     >  2:4317279/1‑93 (MQ=255)

                                                                                    cgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCg    <  1:1559161/93‑1 (MQ=255)

                                                                                    cgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCg    <  1:7318004/93‑1 (MQ=255)

                                                                                      ccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCggg  >  2:6120128/1‑93 (MQ=255)

TCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGG  >  NC_000913/3251050‑3251228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCT  >  NC_000913/3251047‑3251232

CGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAC                                                                                              >  SRR4302543.6915905/1‑94 (MQ=60)

CGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAC                                                                                              <  SRR4302543.484037/94‑1 (MQ=60)

 GATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACC                                                                                             >  SRR4302543.1124714/1‑94 (MQ=60)

  ATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCCCGCCAACCC                                                                                            >  SRR4302543.2349615/1‑94 (MQ=60)

   TCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCC                                                                                           <  SRR4302543.1930731/94‑1 (MQ=60)

    CTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCC                                                                                          >  SRR4302543.1950905/1‑94 (MQ=60)

    CTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCC                                                                                          >  SRR4302543.1248144/1‑94 (MQ=60)

    CTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCC                                                                                          >  SRR4302543.2569156/1‑94 (MQ=60)

      CGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGC                                                                                        >  SRR4302543.6398146/1‑94 (MQ=60)

       GCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCTCGCC                                                                                       >  SRR4302543.4750324/1‑94 (MQ=60)

       GCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCGCGCC                                                                                       >  SRR4302543.739994/1‑94 (MQ=60)

       GCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCC                                                                                       >  SRR4302543.5373967/1‑94 (MQ=60)

        CTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCA                                                                                      >  SRR4302543.4921082/1‑94 (MQ=60)

        CTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCA                                                                                      >  SRR4302543.2758150/1‑94 (MQ=60)

          GGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGT                                                                                    <  SRR4302543.4420850/94‑1 (MQ=60)

          GGTCCATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGT                                                                                    <  SRR4302543.4420658/94‑1 (MQ=60)

           GTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTA                                                                                   <  SRR4302543.4567663/94‑1 (MQ=60)

             CGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAAT                                                                                 <  SRR4302543.372318/94‑1 (MQ=60)

              GATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATG                                                                                >  SRR4302543.684075/1‑94 (MQ=60)

              GATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATG                                                                                >  SRR4302543.3498182/1‑94 (MQ=60)

              GATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATG                                                                                <  SRR4302543.4263589/94‑1 (MQ=60)

              GATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATG                                                                                >  SRR4302543.3862890/1‑94 (MQ=60)

              GATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATG                                                                                >  SRR4302543.7206901/1‑94 (MQ=60)

               ATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGC                                                                               >  SRR4302543.375040/1‑94 (MQ=60)

               ATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGC                                                                               >  SRR4302543.293797/1‑94 (MQ=60)

               ATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGC                                                                               >  SRR4302543.246802/1‑94 (MQ=60)

                TTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCC                                                                              >  SRR4302543.410636/1‑94 (MQ=60)

                   CGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGACACGCCAACCCCCGCCAGTAATGCCTGA                                                                           >  SRR4302543.6307815/1‑94 (MQ=60)

                    GGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATATGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGAC                                                                          >  SRR4302543.1509729/1‑94 (MQ=60)

                     GGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACG                                                                         >  SRR4302543.7760571/1‑94 (MQ=60)

                      GCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGT                                                                        >  SRR4302543.8001626/1‑94 (MQ=60)

                        TGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTT                                                                      >  SRR4302543.992312/1‑94 (MQ=60)

                        TGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTT                                                                      >  SRR4302543.2187860/1‑94 (MQ=60)

                        TGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTT                                                                      <  SRR4302543.3732517/94‑1 (MQ=60)

                           CGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATT                                                                   >  SRR4302543.5836844/1‑94 (MQ=60)

                           CGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATT                                                                   >  SRR4302543.6746212/1‑94 (MQ=60)

                            CACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCT                                                                  <  SRR4302543.2197164/94‑1 (MQ=60)

                             ACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTT                                                                 >  SRR4302543.5974869/1‑94 (MQ=60)

                              CACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTA                                                                >  SRR4302543.5756152/1‑94 (MQ=60)

                               ACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCA                                                               <  SRR4302543.1688050/94‑1 (MQ=60)

                                 GCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAAT                                                             >  SRR4302543.4850094/1‑94 (MQ=60)

                                 GCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAAT                                                             >  SRR4302543.6929054/1‑94 (MQ=60)

                                  CAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATC                                                            >  SRR4302543.2191812/1‑94 (MQ=60)

                                  CAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATC                                                            >  SRR4302543.3264749/1‑94 (MQ=60)

                                   AACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCG                                                           >  SRR4302543.2046262/1‑94 (MQ=60)

                                    ACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGT                                                          >  SRR4302543.3662092/1‑94 (MQ=60)

                                    ACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAA‑CCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTG                                                         >  SRR4302543.3440100/1‑94 (MQ=60)

                                      CGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGC                                                        >  SRR4302543.2999207/1‑94 (MQ=60)

                                       GCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCT                                                       <  SRR4302543.4452222/94‑1 (MQ=60)

                                       GCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCAACGCCAACCCCCGCCCGTAATGCCTGACGTTTATCTTCAATGGTGCT                                                       >  SRR4302543.1852168/1‑94 (MQ=60)

                                        CCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTC                                                      >  SRR4302543.6780955/1‑94 (MQ=60)

                                         CCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCA                                                     >  SRR4302543.7969851/1‑94 (MQ=60)

                                         CCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCA                                                     >  SRR4302543.5797161/1‑94 (MQ=60)

                                         CCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCA                                                     >  SRR4302543.1496567/1‑94 (MQ=60)

                                          CTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAC                                                    <  SRR4302543.7989580/94‑1 (MQ=60)

                                          CTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAC                                                    <  SRR4302543.7989581/94‑1 (MQ=60)

                                              CGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTT                                                >  SRR4302543.2458119/1‑94 (MQ=60)

                                               GCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTA                                               >  SRR4302543.7268992/1‑94 (MQ=60)

                                               GCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTA                                               <  SRR4302543.5983975/94‑1 (MQ=60)

                                                CAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTCATGCCTGACGATTATCTTCAATCGTGATCACCGTTAA                                              >  SRR4302543.7836829/1‑94 (MQ=60)

                                                CAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAA                                              >  SRR4302543.4641476/1‑94 (MQ=60)

                                                CAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAA                                              >  SRR4302543.6870381/1‑94 (MQ=60)

                                                 AATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAG                                             <  SRR4302543.4853082/94‑1 (MQ=60)

                                                 AATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAG                                             >  SRR4302543.7347442/1‑94 (MQ=60)

                                                 AATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAG                                             >  SRR4302543.1178018/1‑94 (MQ=60)

                                                  ATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGC                                            >  SRR4302543.3568349/1‑94 (MQ=60)

                                                  ATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGC                                            >  SRR4302543.5769054/1‑94 (MQ=60)

                                                     TCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCG                                         <  SRR4302543.3242182/94‑1 (MQ=60)

                                                       TTTTTCGACCATAGGATACGGCATCGTCCCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGC                                       <  SRR4302543.78991/94‑1 (MQ=60)

                                                            CGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTT                                  >  SRR4302543.1005393/1‑94 (MQ=60)

                                                             GACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTG                                 >  SRR4302543.1052614/1‑94 (MQ=60)

                                                             GACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGCGCTCACCGTTAAGCGCGGCTGTTTG                                 >  SRR4302543.4531633/1‑94 (MQ=60)

                                                              ACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGT                                >  SRR4302543.1461275/1‑94 (MQ=60)

                                                              ACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGT                                >  SRR4302543.2043004/1‑94 (MQ=60)

                                                              ACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGT                                >  SRR4302543.2165231/1‑94 (MQ=60)

                                                              ACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGT                                >  SRR4302543.5682580/1‑94 (MQ=60)

                                                               CCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTC                               >  SRR4302543.756585/1‑94 (MQ=60)

                                                               CCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTC                               >  SRR4302543.3360560/1‑94 (MQ=60)

                                                               CCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTC                               >  SRR4302543.5004298/1‑94 (MQ=60)

                                                                CATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCC                              >  SRR4302543.3037728/1‑94 (MQ=60)

                                                                 ATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAGCGTTAAGCGCGGCTGTTTGTCCA                             >  SRR4302543.5868729/1‑94 (MQ=60)

                                                                 ATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCA                             <  SRR4302543.1988738/94‑1 (MQ=60)

                                                                 ATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCA                             >  SRR4302543.2919794/1‑94 (MQ=60)

                                                                  TAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAG                            >  SRR4302543.1869009/1‑94 (MQ=60)

                                                                    GGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCA                          <  SRR4302543.2265742/94‑1 (MQ=60)

                                                                    GGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCA                          >  SRR4302543.3526710/1‑94 (MQ=60)

                                                                    GGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCA                          <  SRR4302543.2645586/94‑1 (MQ=60)

                                                                    GGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCA                          <  SRR4302543.6636545/94‑1 (MQ=60)

                                                                    GGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCA                          <  SRR4302543.1107709/94‑1 (MQ=60)

                                                                      ATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGC                        >  SRR4302543.5129919/1‑94 (MQ=60)

                                                                      ATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGC                        >  SRR4302543.5070058/1‑94 (MQ=60)

                                                                        ACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTG                      >  SRR4302543.5618030/1‑94 (MQ=60)

                                                                        ACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTG                      >  SRR4302543.7021178/1‑94 (MQ=60)

                                                                          GGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTA                    >  SRR4302543.4958/1‑94 (MQ=60)

                                                                          GGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTA                    <  SRR4302543.4600084/94‑1 (MQ=60)

                                                                          GGCATCGTCGCCACGCCAACCCCCGCCAGTAACGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTA                    >  SRR4302543.6623378/1‑94 (MQ=60)

                                                                           GCATCGTCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTAC                   <  SRR4302543.757192/94‑1 (MQ=60)

                                                                           GCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTAC                   <  SRR4302543.7315446/94‑1 (MQ=60)

                                                                            CATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACG                  >  SRR4302543.4085061/1‑94 (MQ=60)

                                                                            CATCGCCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACG                  <  SRR4302543.2974340/94‑1 (MQ=60)

                                                                            CATCGCCGCAACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACG                  <  SRR4302543.4680217/94‑1 (MQ=60)

                                                                             ATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACaa                 >  SRR4302543.5879661/1‑92 (MQ=60)

                                                                             ATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGG                 >  SRR4302543.2892962/1‑94 (MQ=60)

                                                                             ATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGG                 >  SRR4302543.1382580/1‑94 (MQ=60)

                                                                             ATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGG                 >  SRR4302543.1153221/1‑94 (MQ=60)

                                                                             ATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGG                 >  SRR4302543.1552396/1‑94 (MQ=60)

                                                                             ATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGG                 >  SRR4302543.7819464/1‑94 (MQ=60)

                                                                             ATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGG                 >  SRR4302543.7817207/1‑94 (MQ=60)

                                                                             ATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGG                 >  SRR4302543.1766206/1‑94 (MQ=60)

                                                                             ATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGG                 >  SRR4302543.4438743/1‑94 (MQ=60)

                                                                             ATCGGCGCCCCGCCAACCCCCGCCAGTAATGCCTTACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGG                 <  SRR4302543.2348577/94‑1 (MQ=60)

                                                                              TCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGT                >  SRR4302543.7360021/1‑94 (MQ=60)

                                                                               CGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTC               >  SRR4302543.2796468/1‑94 (MQ=60)

                                                                                GTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCA              >  SRR4302543.7327553/1‑94 (MQ=60)

                                                                                 TCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAA             <  SRR4302543.1299483/94‑1 (MQ=60)

                                                                                 TCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAA             <  SRR4302543.969807/94‑1 (MQ=60)

                                                                                 TCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAA             >  SRR4302543.5723266/1‑94 (MQ=60)

                                                                                  CGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAAC            <  SRR4302543.1555104/94‑1 (MQ=60)

                                                                                    CCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACAC          <  SRR4302543.3325727/94‑1 (MQ=60)

                                                                                     CACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACC         <  SRR4302543.6352996/94‑1 (MQ=60)

                                                                                     CACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACC         <  SRR4302543.1357355/94‑1 (MQ=60)

                                                                                      ACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCG        >  SRR4302543.3786072/1‑94 (MQ=60)

                                                                                       CGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGG       <  SRR4302543.47977/94‑1 (MQ=60)

                                                                                       CGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGG       <  SRR4302543.1573090/94‑1 (MQ=60)

                                                                                       CGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGG       <  SRR4302543.7388505/94‑1 (MQ=60)

                                                                                       CGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGG       <  SRR4302543.4224526/94‑1 (MQ=60)

                                                                                       CGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGG       <  SRR4302543.3552667/94‑1 (MQ=60)

                                                                                        GCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGG      >  SRR4302543.7003998/1‑94 (MQ=60)

                                                                                        GCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGG      <  SRR4302543.5977349/94‑1 (MQ=60)

                                                                                        GCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGG      >  SRR4302543.940220/1‑94 (MQ=60)

                                                                                          CAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCG    >  SRR4302543.4165038/1‑94 (MQ=60)

                                                                                            ACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCT  <  SRR4302543.4905910/94‑1 (MQ=60)

CGATCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGCGCT  >  NC_000913/3251047‑3251232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`