Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,557 2 bp→GC coding (451‑452/930 nt) ilvE → branched‑chain amino‑acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,5570CG100.0% 1064.5 / NA 334R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (146/188);  total (146/188)
*NC_0009133,950,5580GC100.0% 1039.3 / NA 334R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (146/188);  total (146/188)

Only 100 of 341 total aligned reads displayed.

TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950471‑3950644
                                                                                      ||                                                                                      
 cGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCaaa                                                                                  >  2:1959175/1‑93 (MQ=255)
 cGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCaaa                                                                                  >  1:6436601/1‑93 (MQ=255)
  gctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAac                                                                                 >  1:919178/1‑93 (MQ=255)
  gctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAac                                                                                 >  1:309869/1‑93 (MQ=255)
   ctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAaca                                                                                >  2:2277701/1‑93 (MQ=255)
     gctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAcc                                                                              >  1:3045878/1‑93 (MQ=255)
      ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                             <  1:6713608/93‑1 (MQ=255)
      ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                             <  1:3464341/93‑1 (MQ=255)
      ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                             >  1:2543584/1‑93 (MQ=255)
       tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                            <  1:2147685/93‑1 (MQ=255)
       tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                            <  1:3204988/93‑1 (MQ=255)
        ttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATc                                                                           >  2:6484025/1‑93 (MQ=255)
          cccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATccc                                                                         <  2:2696224/93‑1 (MQ=255)
           ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                        <  1:1401057/93‑1 (MQ=255)
           ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                        <  2:7172799/93‑1 (MQ=255)
            cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                       <  2:7278401/93‑1 (MQ=255)
            cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                       <  1:3866850/93‑1 (MQ=255)
             gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc                                                                      >  2:3169319/1‑93 (MQ=255)
             gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc                                                                      <  1:6603039/93‑1 (MQ=255)
             gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGCGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc                                                                      <  1:7460474/93‑1 (MQ=255)
              tGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcg                                                                     >  2:4682879/1‑93 (MQ=255)
               ggggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcgg                                                                    >  2:384981/1‑93 (MQ=255)
               ggggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcgg                                                                    <  2:4771464/93‑1 (MQ=255)
                   aGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATAGATGCGATGGTTTCCTCCTGGAACCGCGAAGCACCAAACACCATCCCGACggcggc                                                                >  2:2726059/1‑93 (MQ=255)
                   aGCGTATCTGGGCGCAGAAGCGCTGGAACAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACggcggc                                                                >  1:7014232/1‑93 (MQ=255)
                    gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa                                                               >  2:2376655/1‑93 (MQ=255)
                    gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa                                                               >  1:4440536/1‑93 (MQ=255)
                      gTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa                                                             >  2:4121881/1‑93 (MQ=255)
                      cgaTCGGGGAGAAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa                                                             <  2:1529073/91‑1 (MQ=255)
                       tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                            <  2:4352204/93‑1 (MQ=255)
                       tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                            >  1:4676878/1‑93 (MQ=255)
                       tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                            >  2:3315646/1‑93 (MQ=255)
                        aTCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCTTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaac                                                           >  1:5240132/1‑92 (MQ=255)
                        aTCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAg                                                           >  1:474024/1‑93 (MQ=255)
                         tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                          >  2:5839412/1‑93 (MQ=255)
                         tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                          >  2:2183330/1‑93 (MQ=255)
                         tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                          <  1:3260269/93‑1 (MQ=255)
                           tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                        >  2:5358592/1‑93 (MQ=255)
                           tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                        <  1:1109035/93‑1 (MQ=255)
                             ggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggt                                                      <  1:7801553/93‑1 (MQ=255)
                              gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtg                                                     <  2:4472254/93‑1 (MQ=255)
                                gcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtggt                                                   <  1:2786892/93‑1 (MQ=255)
                                 cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTa                                                  <  1:7109563/93‑1 (MQ=255)
                                  aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTaa                                                 <  2:1839403/93‑1 (MQ=255)
                                   gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                >  1:3728238/1‑93 (MQ=255)
                                   gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                >  1:1305153/1‑93 (MQ=255)
                                     aGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTa                                              >  2:2630996/1‑93 (MQ=255)
                                      gcgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAc                                             >  2:810336/1‑93 (MQ=255)
                                      gcgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAc                                             <  2:375113/93‑1 (MQ=255)
                                          tGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctct                                         <  1:1604667/93‑1 (MQ=255)
                                           ggAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctt                                        >  2:6809355/1‑92 (MQ=255)
                                           ggAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc                                        <  2:4918697/93‑1 (MQ=255)
                                           ggAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc                                        <  2:5157334/93‑1 (MQ=255)
                                            gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct                                       >  2:4512613/1‑93 (MQ=255)
                                            gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct                                       <  1:1073685/93‑1 (MQ=255)
                                             aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCtt                                      <  1:6150778/93‑1 (MQ=255)
                                              gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc                                     <  2:7879383/93‑1 (MQ=255)
                                              gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc                                     <  2:2067287/93‑1 (MQ=255)
                                              gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc                                     >  2:116768/1‑93 (MQ=255)
                                              gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc                                     >  2:1105584/1‑93 (MQ=255)
                                                aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGGGGTAACTACCTCTCTTccc                                   >  2:6600313/1‑93 (MQ=255)
                                                aGGGGATCAATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTccc                                   >  1:4263091/1‑93 (MQ=255)
                                                 ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCct                                  <  1:2316450/93‑1 (MQ=255)
                                                    gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct                               >  1:5565404/1‑93 (MQ=255)
                                                     aTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgctg                              <  2:838449/93‑1 (MQ=255)
                                                     aTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgctg                              >  2:594102/1‑93 (MQ=255)
                                                        gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           >  1:1212239/1‑93 (MQ=255)
                                                        gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           >  1:5777666/1‑93 (MQ=255)
                                                         atgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGGCGGCGGCAAAAGCCGGTGGTAACTATCTCTCTTCCCTGCtggttg                          >  2:6296206/1‑91 (MQ=255)
                                                         atgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg                          >  2:6340192/1‑93 (MQ=255)
                                                         atgcgaagGTTTCCTCAAGGAACCGCGCAGCACCAAACACCAACCCGACGGCGGCAAAAGCCGGTGGTAACTACCACTCTTCCCTGCtggtgg                          <  2:7034334/93‑1 (MQ=255)
                                                          tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg                         <  1:5493206/93‑1 (MQ=255)
                                                          tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg                         >  1:1677625/1‑93 (MQ=255)
                                                            cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                       <  2:863811/93‑1 (MQ=255)
                                                            cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                       <  1:7623456/93‑1 (MQ=255)
                                                            cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                       <  1:510073/93‑1 (MQ=255)
                                                              atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                     >  1:273254/1‑93 (MQ=255)
                                                              atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                     >  1:3907670/1‑93 (MQ=255)
                                                               tgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTCACTACCTCTCTTCCCTGCTGGTGGGTAGCg                    >  1:3560619/1‑93 (MQ=255)
                                                               tgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCg                    >  2:1309785/1‑93 (MQ=255)
                                                                ggTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGa                   >  2:7687952/1‑93 (MQ=255)
                                                                 gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                  <  2:1684135/93‑1 (MQ=255)
                                                                 gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                  <  1:7331709/93‑1 (MQ=255)
                                                                  tttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAg                 >  1:7665860/1‑93 (MQ=255)
                                                                  tttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAg                 <  2:6406667/93‑1 (MQ=255)
                                                                  tcccctcctGGAACCGCGCAGCACCAAACCCCACCCCGACGGCGCCAAAAGCCGGTGGTACCTACCTCTCTTCCCTGGTGGTGGGTAGCGAAg                 <  2:6914619/90‑1 (MQ=255)
                                                                    tcctccTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcg               <  2:7738280/93‑1 (MQ=255)
                                                                     cctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgc              >  2:2986966/1‑93 (MQ=255)
                                                                      ctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcg             <  1:1768218/93‑1 (MQ=255)
                                                                         ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTCACTACCTCTCTTCCCTGCTGGTGGGAAGCGAAGCGcgccg          >  1:6672308/1‑93 (MQ=255)
                                                                           ggAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCgccgcc        >  2:1232858/1‑93 (MQ=255)
                                                                           ggAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCgccgcc        <  1:2005442/93‑1 (MQ=255)
                                                                            gAACCGCGCAGCACCAAACACCCTCCCGACGGCGGCAAAAGCCGGCGGTAACTACCTCTCTTCCCGGCTGGTGCGTAGCGAAACAcggcgcac       >  2:3391971/1‑91 (MQ=255)
                                                                            gAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa       <  1:2621989/93‑1 (MQ=255)
                                                                             aaCCGCACAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAc      <  2:5047145/93‑1 (MQ=255)
                                                                              aCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACg     >  2:737630/1‑93 (MQ=255)
                                                                              aCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACg     >  2:3090436/1‑93 (MQ=255)
                                                                               ccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACgg    <  1:4110873/93‑1 (MQ=255)
                                                                                cgcgCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGt   <  1:1825383/93‑1 (MQ=255)
                                                                                 gcgcAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGtt  <  2:3621123/93‑1 (MQ=255)
                                                                                      ||                                                                                      
TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950471‑3950644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950471‑3950644
                                                                                      |                                                                                       
TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAA                                                                                  <  SRR4302543.2159742/94‑1 (MQ=60)
TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAA                                                                                  >  SRR4302543.6497247/1‑94 (MQ=60)
TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAA                                                                                  >  SRR4302543.7612999/1‑94 (MQ=60)
 CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                 >  SRR4302543.927227/1‑94 (MQ=60)
 CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                 <  SRR4302543.474895/94‑1 (MQ=60)
 CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                 >  SRR4302543.312660/1‑94 (MQ=60)
  GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                <  SRR4302543.1127654/94‑1 (MQ=60)
  GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                <  SRR4302543.3801274/94‑1 (MQ=60)
  GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                >  SRR4302543.6096690/1‑94 (MQ=60)
   CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                               <  SRR4302543.313726/94‑1 (MQ=60)
   CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                               <  SRR4302543.6472009/94‑1 (MQ=60)
    TGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCATACACC                                                                              <  SRR4302543.7910898/94‑1 (MQ=60)
    TGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACC                                                                              >  SRR4302543.3073337/1‑94 (MQ=60)
    TGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACC                                                                              >  SRR4302543.1650009/1‑94 (MQ=60)
    TGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACC                                                                              >  SRR4302543.7637111/1‑94 (MQ=60)
     GCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCA                                                                             <  SRR4302543.7285852/94‑1 (MQ=60)
     GCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCA                                                                             >  SRR4302543.2566778/1‑94 (MQ=60)
      CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            <  SRR4302543.575891/94‑1 (MQ=60)
      CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            <  SRR4302543.1284495/94‑1 (MQ=60)
      CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            <  SRR4302543.6777048/94‑1 (MQ=60)
      CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            <  SRR4302543.3495372/94‑1 (MQ=60)
      CTTGCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                            <  SRR4302543.4646600/94‑1 (MQ=60)
       TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCGCCAAACACCATC                                                                           <  SRR4302543.6884958/94‑1 (MQ=60)
       TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                           <  SRR4302543.2562114/94‑1 (MQ=60)
       TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                           <  SRR4302543.2166913/94‑1 (MQ=60)
       TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                           <  SRR4302543.6852496/94‑1 (MQ=60)
       TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                           <  SRR4302543.3233902/94‑1 (MQ=60)
        TTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCC                                                                          <  SRR4302543.6534661/94‑1 (MQ=60)
         TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                         <  SRR4302543.5608517/94‑1 (MQ=60)
          CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                        <  SRR4302543.2368319/94‑1 (MQ=60)
          CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                        >  SRR4302543.6597724/1‑94 (MQ=60)
          CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                        >  SRR4302543.3121625/1‑94 (MQ=60)
           CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                       <  SRR4302543.1413702/94‑1 (MQ=60)
           CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                       >  SRR4302543.7601694/1‑94 (MQ=60)
            CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                      <  SRR4302543.3901006/94‑1 (MQ=60)
            CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                      <  SRR4302543.1291103/94‑1 (MQ=60)
            CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                      <  SRR4302543.3708659/94‑1 (MQ=60)
            CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                      >  SRR4302543.2790414/1‑94 (MQ=60)
             GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                     >  SRR4302543.4152517/1‑94 (MQ=60)
             GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                     >  SRR4302543.1932245/1‑94 (MQ=60)
             GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                     <  SRR4302543.6665264/94‑1 (MQ=60)
             GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                     >  SRR4302543.7212438/1‑94 (MQ=60)
             GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGCGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                     <  SRR4302543.7531938/94‑1 (MQ=60)
               GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGC                                                                   >  SRR4302543.5376221/1‑94 (MQ=60)
                GGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCG                                                                  <  SRR4302543.1996581/94‑1 (MQ=60)
                GGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCG                                                                  >  SRR4302543.7468970/1‑94 (MQ=60)
                 GGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGG                                                                 <  SRR4302543.263124/94‑1 (MQ=60)
                  GAGCGTATCTGGGCGCAGAAGCGCTGGAACAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC                                                                >  SRR4302543.7081192/1‑94 (MQ=60)
                  GAGCGTATCTGGGCGCAGAAGCGCCGGAACAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAATACCATCCCGACGGCGGC                                                                >  SRR4302543.7081193/1‑94 (MQ=60)
                   AGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCA                                                               >  SRR4302543.4480747/1‑94 (MQ=60)
                     CGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAA                                                             <  SRR4302543.4064906/94‑1 (MQ=60)
                     CGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAA                                                             >  SRR4302543.2725170/1‑94 (MQ=60)
                     CGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAA                                                             <  SRR4302543.6644913/94‑1 (MQ=60)
                     CGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAA                                                             >  SRR4302543.1749074/1‑94 (MQ=60)
                      GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                            >  SRR4302543.3497743/1‑94 (MQ=60)
                      GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                            >  SRR4302543.3600217/1‑94 (MQ=60)
                      GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                            >  SRR4302543.4719516/1‑94 (MQ=60)
                       TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCTTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAC                                                           >  SRR4302543.5287357/1‑94 (MQ=60)
                       TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                           >  SRR4302543.478410/1‑94 (MQ=60)
                       TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                           >  SRR4302543.5533424/1‑94 (MQ=60)
                       TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                           <  SRR4302543.5576023/94‑1 (MQ=60)
                       TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                           <  SRR4302543.8007830/94‑1 (MQ=60)
                       TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                           >  SRR4302543.4410451/1‑94 (MQ=60)
                       TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                           <  SRR4302543.7721533/94‑1 (MQ=60)
                         TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                         <  SRR4302543.3289520/94‑1 (MQ=60)
                         TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                         <  SRR4302543.1104271/94‑1 (MQ=60)
                         TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                         <  SRR4302543.3289738/94‑1 (MQ=60)
                          CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                        <  SRR4302543.7822103/94‑1 (MQ=60)
                          CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                        <  SRR4302543.2193450/94‑1 (MQ=60)
                           TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                       <  SRR4302543.3861152/94‑1 (MQ=60)
                           TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                       <  SRR4302543.1119041/94‑1 (MQ=60)
                           TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                       <  SRR4302543.3224149/94‑1 (MQ=60)
                            GGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGT                                                      <  SRR4302543.3935930/94‑1 (MQ=60)
                             GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                     <  SRR4302543.113858/94‑1 (MQ=60)
                             GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                     <  SRR4302543.7875089/94‑1 (MQ=60)
                              GCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGG                                                    <  SRR4302543.147336/94‑1 (MQ=60)
                                GCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                  <  SRR4302543.2812320/94‑1 (MQ=60)
                                 CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                 <  SRR4302543.17544/94‑1 (MQ=60)
                                 CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                 >  SRR4302543.7470431/1‑94 (MQ=60)
                                 CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                 <  SRR4302543.7178632/94‑1 (MQ=60)
                                 CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACAAAACACCATCCCGCCGGCGGCAAAAGCCGGTGGTAA                                                 <  SRR4302543.1353049/94‑1 (MQ=60)
                                 CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTGCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                 <  SRR4302543.7178631/94‑1 (MQ=60)
                                  AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                <  SRR4302543.4656491/94‑1 (MQ=60)
                                  AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                >  SRR4302543.6727530/1‑94 (MQ=60)
                                  AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                >  SRR4302543.3761025/1‑94 (MQ=60)
                                  AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                <  SRR4302543.3232625/94‑1 (MQ=60)
                                  AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                <  SRR4302543.2690206/94‑1 (MQ=60)
                                  AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                >  SRR4302543.1316747/1‑94 (MQ=60)
                                   GAAGCGCCGGAGCAGGGGATCGATGCGATGGTTTCCTCCTCGACCCGAGCAGCACCAACCCCCACCCCGACGGCGGCAAAAGCCGGTGGTAACT                                               <  SRR4302543.5366432/94‑1 (MQ=53)
                                    AAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTA                                              >  SRR4302543.5012467/1‑94 (MQ=60)
                                     AGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAC                                             <  SRR4302543.3421867/94‑1 (MQ=60)
                                      GCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACC                                            >  SRR4302543.3955579/1‑94 (MQ=60)
                                       CGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCT                                           <  SRR4302543.517466/94‑1 (MQ=60)
                                         CTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                         <  SRR4302543.1792387/94‑1 (MQ=60)
                                         CTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                         <  SRR4302543.4765047/94‑1 (MQ=60)
                                          TGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTC                                        <  SRR4302543.1618988/94‑1 (MQ=60)
                                          TGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTC                                        <  SRR4302543.6746100/94‑1 (MQ=60)
                                          TGGAGCAGGGGATCGATGCGATGGTTGCCTCCTTGAACCGCGCACCAACAAACACCTTCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTC                                        <  SRR4302543.1618989/94‑1 (MQ=60)
                                           GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                       <  SRR4302543.2925012/94‑1 (MQ=60)
                                           GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                       <  SRR4302543.4344738/94‑1 (MQ=60)
                                           GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                       <  SRR4302543.4070536/94‑1 (MQ=60)
                                           GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                       >  SRR4302543.6738314/1‑94 (MQ=60)
                                            GAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTT                                      <  SRR4302543.1083335/94‑1 (MQ=60)
                                            GAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTT                                      <  SRR4302543.2592350/94‑1 (MQ=60)
                                             AGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTC                                     <  SRR4302543.6209017/94‑1 (MQ=60)
                                               CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                   >  SRR4302543.2060831/1‑94 (MQ=60)
                                               CAGGGGATCAATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                   >  SRR4302543.4301812/1‑94 (MQ=60)
                                                 GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                 >  SRR4302543.5034612/1‑94 (MQ=60)
                                                 GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                 <  SRR4302543.2337512/94‑1 (MQ=60)
                                                  GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                >  SRR4302543.1236217/1‑94 (MQ=60)
                                                   GGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCT                               >  SRR4302543.5616031/1‑94 (MQ=60)
                                                    GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                              >  SRR4302543.332557/1‑94 (MQ=60)
                                                     ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                             <  SRR4302543.5237064/94‑1 (MQ=60)
                                                      TCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGT                            <  SRR4302543.7704527/94‑1 (MQ=60)
                                                      TCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGT                            <  SRR4302543.3913059/94‑1 (MQ=60)
                                                      TCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGT                            >  SRR4302543.3098953/1‑94 (MQ=60)
                                                       CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                           >  SRR4302543.5830258/1‑94 (MQ=60)
                                                       CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                           >  SRR4302543.1223143/1‑94 (MQ=60)
                                                         ATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGG                         >  SRR4302543.1692665/1‑94 (MQ=60)
                                                          TGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGT                        <  SRR4302543.5543028/94‑1 (MQ=60)
                                                          TGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGT                        <  SRR4302543.1791152/94‑1 (MQ=60)
                                                            CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                      <  SRR4302543.7695804/94‑1 (MQ=60)
                                                            CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                      <  SRR4302543.514819/94‑1 (MQ=60)
                                                            CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                      <  SRR4302543.6433258/94‑1 (MQ=60)
                                                             GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                     >  SRR4302543.275654/1‑94 (MQ=60)
                                                             GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                     >  SRR4302543.6993199/1‑94 (MQ=60)
                                                             GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                     >  SRR4302543.3942160/1‑94 (MQ=60)
                                                             GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGAGGTAACTACCTCTCTTCCCTGCTGGTGCGTAGG                     >  SRR4302543.2314439/1‑94 (MQ=60)
                                                              ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTCACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    >  SRR4302543.3592148/1‑94 (MQ=60)
                                                              ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    >  SRR4302543.4211229/1‑94 (MQ=60)
                                                              ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    <  SRR4302543.6686381/94‑1 (MQ=60)
                                                              ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    <  SRR4302543.3476223/94‑1 (MQ=60)
                                                              ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    <  SRR4302543.7930081/94‑1 (MQ=60)
                                                              ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    <  SRR4302543.4966795/94‑1 (MQ=60)
                                                              ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                    >  SRR4302543.5759882/1‑94 (MQ=60)
                                                                TGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAA                  <  SRR4302543.7861342/94‑1 (MQ=60)
                                                                TGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAA                  <  SRR4302543.6034488/94‑1 (MQ=60)
                                                                 GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                 <  SRR4302543.7402264/94‑1 (MQ=60)
                                                                 GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                 >  SRR4302543.7738515/1‑94 (MQ=60)
                                                                 GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                 <  SRR4302543.365673/94‑1 (MQ=60)
                                                                    TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC              >  SRR4302543.3501731/1‑94 (MQ=60)
                                                                    TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC              <  SRR4302543.2157372/94‑1 (MQ=60)
                                                                      CTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGC            <  SRR4302543.1784030/94‑1 (MQ=60)
                                                                      CTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGC            >  SRR4302543.1579814/1‑94 (MQ=60)
                                                                        CCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTCACTACCTCTCTTCCCTGCTGGTGGGAAGCGAAGCGCGCCG          >  SRR4302543.6735305/1‑94 (MQ=60)
                                                                        CCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCGG          >  SRR4302543.1332586/1‑94 (MQ=60)
                                                                        CCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCG          >  SRR4302543.2759535/1‑94 (MQ=60)
                                                                         CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGC         >  SRR4302543.6052267/1‑94 (MQ=60)
                                                                          TGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCC        >  SRR4302543.4349009/1‑94 (MQ=60)
                                                                          TGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCC        >  SRR4302543.7253963/1‑94 (MQ=60)
                                                                           GGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA       <  SRR4302543.2023463/94‑1 (MQ=60)
                                                                           GGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA       >  SRR4302543.276815/1‑94 (MQ=60)
                                                                            GAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAC      <  SRR4302543.2645736/94‑1 (MQ=60)
                                                                             AACCGCGCAGCACCAAACACCATCCGGACGGCGGCAAAAGCCGGTGGTAACTCCCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG     <  SRR4302543.1604276/94‑1 (MQ=60)
                                                                             AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG     >  SRR4302543.327530/1‑94 (MQ=60)
                                                                             AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG     >  SRR4302543.1839653/1‑94 (MQ=60)
                                                                              ACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGTTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGG    <  SRR4302543.2870985/94‑1 (MQ=60)
                                                                              ACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGG    >  SRR4302543.547449/1‑94 (MQ=60)
                                                                               CCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT   <  SRR4302543.4147872/94‑1 (MQ=60)
                                                                               CCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT   <  SRR4302543.1085722/94‑1 (MQ=60)
                                                                                CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  <  SRR4302543.1841771/94‑1 (MQ=60)
                                                                                CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  <  SRR4302543.261060/94‑1 (MQ=60)
                                                                                CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  SRR4302543.1913356/1‑94 (MQ=60)
                                                                                CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  SRR4302543.3682779/1‑94 (MQ=60)
                                                                                      |                                                                                       
TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950471‑3950644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑