Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,972,637 2 bp→GC coding (430‑431/546 nt) wecD → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,972,6370CG100.0% 1054.1 / NA 327R144A (CGT→GCT) wecDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (136/191);  total (136/191)
*NC_0009133,972,6380GC100.0% 1040.6 / NA 327R144A (CGT→GCT) wecDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (136/191);  total (136/191)

Only 100 of 332 total aligned reads displayed.

AATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972553‑3972724
                                                                                    ||                                                                                      
aaTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGt                                                                                 >  1:5138336/1‑93 (MQ=255)
aaTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGt                                                                                 >  2:1741553/1‑93 (MQ=255)
aaTGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGt                                                                                 >  2:2684884/1‑93 (MQ=255)
    cGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTaaaa                                                                             <  1:3268686/93‑1 (MQ=255)
     gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaac                                                                            <  1:3894576/93‑1 (MQ=255)
     gACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaac                                                                            <  2:311421/93‑1 (MQ=255)
       cAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaacaa                                                                          >  1:4892190/1‑93 (MQ=255)
        aGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaacaac                                                                         <  1:1254043/93‑1 (MQ=255)
        aGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAaacaac                                                                         >  1:2833105/1‑93 (MQ=255)
         taTGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACt                                                                        >  2:293241/2‑93 (MQ=255)
         gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACt                                                                        >  1:445074/1‑93 (MQ=255)
         gATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACt                                                                        <  2:7336844/93‑1 (MQ=255)
          aTGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACtt                                                                       <  1:6168017/93‑1 (MQ=255)
          aTGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACtt                                                                       >  2:6435908/1‑93 (MQ=255)
           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTTCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACttt                                                                      <  1:2536275/93‑1 (MQ=255)
           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTGTGCTAACGGTAAAACAGCtta                                                                      <  2:7027850/93‑2 (MQ=255)
           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACttt                                                                      <  2:232566/93‑1 (MQ=255)
           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACttt                                                                      <  1:6625073/93‑1 (MQ=255)
           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACttt                                                                      <  2:5587729/93‑1 (MQ=255)
           tGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACttt                                                                      >  2:1691192/1‑93 (MQ=255)
            gcgcGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTg                                                                     <  2:4037819/93‑1 (MQ=255)
            gcgcGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTg                                                                     <  2:4924519/93‑1 (MQ=255)
              gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCg                                                                   <  2:725346/93‑1 (MQ=255)
              gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCg                                                                   <  2:1878063/93‑1 (MQ=255)
              gcgAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCg                                                                   <  1:1165992/93‑1 (MQ=255)
                gAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCggg                                                                 >  2:3917643/1‑93 (MQ=255)
                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCgggg                                                                >  2:4193743/1‑92 (MQ=255)
                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGt                                                                <  1:6566948/93‑1 (MQ=255)
                 aaTTGGCCTGCTGGCTGGACGCGGTGCAGGTGCCGAGCTGATGCAAACAGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTATGCgggc                                                                >  2:7861340/1‑92 (MQ=255)
                   ttGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTgg                                                              <  1:7078763/93‑1 (MQ=255)
                      gCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGa                                                           <  1:731874/93‑1 (MQ=255)
                         tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAccc                                                        >  1:7504369/1‑93 (MQ=255)
                         tgctgGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGAGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAccc                                                        >  1:4365977/1‑93 (MQ=255)
                            tggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCaaa                                                     <  1:4777918/93‑1 (MQ=255)
                             ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAt                                                    <  1:5643412/93‑1 (MQ=255)
                             ggctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAt                                                    <  2:3483247/93‑1 (MQ=255)
                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   >  1:1930908/1‑93 (MQ=255)
                              gctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATg                                                   >  2:2412520/1‑93 (MQ=255)
                               ctggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATgg                                                  <  2:6683167/93‑1 (MQ=255)
                                 ggACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGc                                                <  2:7527434/93‑1 (MQ=255)
                                   aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa                                              <  1:405021/93‑1 (MQ=255)
                                   aCGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCaa                                              >  1:4509569/1‑93 (MQ=255)
                                       ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc                                          <  2:1326064/93‑1 (MQ=255)
                                       ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc                                          <  2:5321516/93‑1 (MQ=255)
                                       ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc                                          <  1:3360573/93‑1 (MQ=255)
                                       ggTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAcc                                          >  2:6270116/1‑93 (MQ=255)
                                        gTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccg                                         >  2:859190/1‑93 (MQ=255)
                                         tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgc                                        <  2:6065452/93‑1 (MQ=255)
                                         tGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACAccgc                                        >  2:479468/1‑93 (MQ=255)
                                             ggTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCcgcg                                    >  1:6269419/1‑93 (MQ=255)
                                              gTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCgcgc                                   <  2:2037890/93‑1 (MQ=255)
                                               tGCGGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCt                                  <  1:2175395/93‑1 (MQ=255)
                                                gctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCtt                                 <  1:825347/93‑1 (MQ=255)
                                                 ctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTa                                <  1:5878360/93‑1 (MQ=255)
                                                 ctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTa                                <  2:6957012/93‑1 (MQ=255)
                                                 ctgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTa                                >  2:3642974/1‑93 (MQ=255)
                                                  tgagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaa                               <  1:3119683/93‑1 (MQ=255)
                                                   gagctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTaaa                              >  2:3295175/1‑93 (MQ=255)
                                                     gctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACg                            <  2:5784044/93‑1 (MQ=255)
                                                      ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAATGa                           <  1:4090702/93‑1 (MQ=255)
                                                      ctgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGa                           >  1:6673486/1‑93 (MQ=255)
                                                       tgaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGat                          >  2:6069442/1‑93 (MQ=255)
                                                        gaTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGata                         >  1:5365215/1‑93 (MQ=255)
                                                         aTGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatac                        >  1:7809967/1‑93 (MQ=255)
                                                          tGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGataca                       <  2:2579100/93‑1 (MQ=255)
                                                             aaaCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacatac                    >  2:5970804/1‑93 (MQ=255)
                                                             aaaCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGatacatac                    >  2:4583233/1‑93 (MQ=255)
                                                              aaCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   >  1:5063889/1‑93 (MQ=255)
                                                              aaCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   <  2:1422078/93‑1 (MQ=255)
                                                              aaCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAtacataca                   >  1:167897/1‑93 (MQ=255)
                                                               aCGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaa                  >  2:7876549/1‑93 (MQ=255)
                                                                cGGGGCTAACCTGCGCGTATGCTCACGGTAAACCAACTTTGCGGTTGGCGACCCAAATGGGCAGCACCGCCGCGCTTAAACGATACATGCaaa                 <  2:3209791/93‑1 (MQ=255)
                                                                cGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaaa                 <  2:3406845/93‑1 (MQ=255)
                                                                cGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACaaa                 <  1:2328554/93‑1 (MQ=255)
                                                                 ggCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAg                <  1:6949283/93‑1 (MQ=255)
                                                                 ggCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAg                <  1:2715937/93‑1 (MQ=255)
                                                                  gcgcTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgt               <  2:5482845/93‑1 (MQ=255)
                                                                  gcgcTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgt               <  1:1442268/93‑1 (MQ=255)
                                                                   cgcTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtg              <  1:4658514/93‑1 (MQ=255)
                                                                    gcTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  2:4608181/1‑93 (MQ=255)
                                                                    gcTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  2:5138298/1‑93 (MQ=255)
                                                                    gcTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             >  2:1209459/1‑93 (MQ=255)
                                                                    gcTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtgg             <  2:4409456/93‑1 (MQ=255)
                                                                     cTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  1:913863/93‑1 (MQ=255)
                                                                     cTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  2:7652685/93‑1 (MQ=255)
                                                                     cTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            >  1:7576441/1‑93 (MQ=255)
                                                                     cTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            >  1:6772886/1‑93 (MQ=255)
                                                                     cTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggt            <  2:6821444/93‑1 (MQ=255)
                                                                      tAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggtg           <  2:2870671/93‑1 (MQ=255)
                                                                      tAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAgtggtg           <  2:7822691/93‑1 (MQ=255)
                                                                       aaaCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGc          <  2:3828653/93‑1 (MQ=255)
                                                                       aaaCTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGc          <  2:7429618/93‑1 (MQ=255)
                                                                          cTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAGGATACATACAAAGTGGTGCGaa       <  2:4980300/93‑1 (MQ=255)
                                                                          cTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGaa       >  1:5204063/1‑93 (MQ=255)
                                                                          cTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGaa       <  1:2886161/93‑1 (MQ=255)
                                                                           tGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAt      <  1:6483223/93‑1 (MQ=255)
                                                                            gggCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATg     <  2:4326178/93‑1 (MQ=255)
                                                                            gggCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATg     <  1:5952718/93‑1 (MQ=255)
                                                                            gggCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATg     <  1:2081804/93‑1 (MQ=255)
                                                                             ggCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGt    <  1:2786036/93‑1 (MQ=255)
                                                                                    ||                                                                                      
AATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG  >  NC_000913/3972553‑3972724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGAACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAG  >  NC_000913/3972544‑3972728
                                                                                             |                                                                                           
CGGGAACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATG                                                                                             >  SRR4302543.2908339/1‑94 (MQ=60)
CGGGAACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATG                                                                                             >  SRR4302543.1913568/1‑94 (MQ=60)
          ATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAA                                                                                   >  SRR4302543.6615998/1‑94 (MQ=60)
          ATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAA                                                                                   >  SRR4302543.231218/1‑94 (MQ=60)
           TGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAA                                                                                  <  SRR4302543.7599990/94‑1 (MQ=60)
           TGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAA                                                                                  >  SRR4302543.4931896/1‑94 (MQ=60)
            GCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAA                                                                                 <  SRR4302543.7265250/94‑1 (MQ=60)
            GCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAA                                                                                 >  SRR4302543.4678014/1‑94 (MQ=60)
             CGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAAC                                                                                >  SRR4302543.3396081/1‑94 (MQ=60)
             CGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAAC                                                                                >  SRR4302543.21605/1‑94 (MQ=60)
             CGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAAC                                                                                <  SRR4302543.3298220/94‑1 (MQ=60)
              GACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACA                                                                               >  SRR4302543.4600733/1‑94 (MQ=60)
              GACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACA                                                                               <  SRR4302543.3928961/94‑1 (MQ=60)
               ACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAA                                                                              >  SRR4302543.4936786/1‑94 (MQ=60)
                CAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAAC                                                                             >  SRR4302543.961892/1‑94 (MQ=60)
                CAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAAC                                                                             >  SRR4302543.2859020/1‑94 (MQ=60)
                 AGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACT                                                                            <  SRR4302543.1265282/94‑1 (MQ=60)
                 AGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACT                                                                            <  SRR4302543.7450921/94‑1 (MQ=60)
                 AGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACT                                                                            >  SRR4302543.449243/1‑94 (MQ=60)
                 AGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACT                                                                            <  SRR4302543.1831017/94‑1 (MQ=60)
                  GATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTT                                                                           <  SRR4302543.4045741/94‑1 (MQ=60)
                   ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTT                                                                          <  SRR4302543.1434726/94‑1 (MQ=60)
                   ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTT                                                                          <  SRR4302543.6226375/94‑1 (MQ=60)
                   ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTT                                                                          >  SRR4302543.4158660/1‑94 (MQ=60)
                   ATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAAATGGGCGTATGCTCACGGTAAAACAACTTT                                                                          >  SRR4302543.140335/1‑94 (MQ=60)
                    TGCGCGAATTGGCCTGCTGGCTGGACGCGGTTCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTG                                                                         <  SRR4302543.2559383/94‑1 (MQ=60)
                    TGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTG                                                                         <  SRR4302543.2586122/94‑1 (MQ=60)
                    TGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTG                                                                         <  SRR4302543.6687552/94‑1 (MQ=60)
                       GCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGG                                                                      <  SRR4302543.1176592/94‑1 (MQ=60)
                       GCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGG                                                                      <  SRR4302543.700561/94‑1 (MQ=60)
                       GCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGG                                                                      <  SRR4302543.5083827/94‑1 (MQ=60)
                         GAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGG                                                                    >  SRR4302543.1668171/1‑94 (MQ=60)
                          AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTG                                                                   >  SRR4302543.2918429/1‑94 (MQ=60)
                          AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTG                                                                   >  SRR4302543.206084/1‑94 (MQ=60)
                          AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTG                                                                   <  SRR4302543.6628747/94‑1 (MQ=60)
                          AATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTG                                                                   >  SRR4302543.5934981/1‑94 (MQ=60)
                           ATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGG                                                                  <  SRR4302543.5033426/94‑1 (MQ=60)
                           ATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGG                                                                  <  SRR4302543.1197131/94‑1 (MQ=60)
                           ATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGG                                                                  <  SRR4302543.4120245/94‑1 (MQ=60)
                            TTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGC                                                                 >  SRR4302543.398421/1‑94 (MQ=60)
                            TTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGC                                                                 <  SRR4302543.7147506/94‑1 (MQ=60)
                               GCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAC                                                              <  SRR4302543.738433/94‑1 (MQ=60)
                               GCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAC                                                              <  SRR4302543.1539884/94‑1 (MQ=60)
                               GCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGAC                                                              <  SRR4302543.4935187/94‑1 (MQ=60)
                                CCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACC                                                             <  SRR4302543.736117/94‑1 (MQ=60)
                                 CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCC                                                            >  SRR4302543.7576107/1‑94 (MQ=60)
                                 CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCC                                                            >  SRR4302543.4316813/1‑94 (MQ=60)
                                 CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCC                                                            >  SRR4302543.3322294/1‑94 (MQ=60)
                                 CTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGAGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCC                                                            >  SRR4302543.4405525/1‑94 (MQ=60)
                                     TGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAT                                                        <  SRR4302543.4450958/94‑1 (MQ=60)
                                     TGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAT                                                        <  SRR4302543.3010325/94‑1 (MQ=60)
                                     TGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAAT                                                        <  SRR4302543.4821585/94‑1 (MQ=60)
                                      GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                       <  SRR4302543.1904487/94‑1 (MQ=60)
                                      GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                       >  SRR4302543.1948281/1‑94 (MQ=60)
                                      GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                       <  SRR4302543.6745951/94‑1 (MQ=60)
                                      GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                       <  SRR4302543.5694857/94‑1 (MQ=60)
                                      GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                       <  SRR4302543.2803699/94‑1 (MQ=60)
                                      GGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATG                                                       <  SRR4302543.2803700/94‑1 (MQ=60)
                                       GCTGGAGGCGGTGCATGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACATCTTTGCGGGTGGCGACCCAAATGG                                                      <  SRR4302543.988825/94‑1 (MQ=60)
                                       GCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGG                                                      <  SRR4302543.525896/94‑1 (MQ=60)
                                        CTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGG                                                     >  SRR4302543.3410336/1‑94 (MQ=60)
                                           GACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAA                                                  >  SRR4302543.5657228/1‑94 (MQ=60)
                                           GACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAA                                                  >  SRR4302543.3320771/1‑94 (MQ=60)
                                           GACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAA                                                  <  SRR4302543.7649141/94‑1 (MQ=60)
                                           GACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAA                                                  >  SRR4302543.4550576/1‑94 (MQ=60)
                                            ACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAAC                                                 <  SRR4302543.6450734/94‑1 (MQ=60)
                                            ACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAAC                                                 <  SRR4302543.408758/94‑1 (MQ=60)
                                             CGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACA                                                <  SRR4302543.318749/94‑1 (MQ=60)
                                                GGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCG                                             <  SRR4302543.3390796/94‑1 (MQ=60)
                                                GGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCC                                             >  SRR4302543.4696030/1‑94 (MQ=60)
                                                 GTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGC                                            >  SRR4302543.4497160/1‑94 (MQ=60)
                                                  TGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCC                                           <  SRR4302543.4530443/94‑1 (MQ=60)
                                                    CAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGC                                         >  SRR4302543.6079763/1‑94 (MQ=60)
                                                    CAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGC                                         >  SRR4302543.5497195/1‑94 (MQ=60)
                                                    CAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGC                                         >  SRR4302543.5194641/1‑94 (MQ=60)
                                                     AGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCG                                        >  SRR4302543.6328542/1‑94 (MQ=60)
                                                       GTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCT                                      <  SRR4302543.1249586/94‑1 (MQ=60)
                                                       GTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCT                                      <  SRR4302543.7002406/94‑1 (MQ=60)
                                                        TGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTT                                     <  SRR4302543.2194886/94‑1 (MQ=60)
                                                        TGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTT                                     >  SRR4302543.7461200/1‑94 (MQ=60)
                                                        TGCGGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTT                                     <  SRR4302543.2194885/94‑1 (MQ=60)
                                                         GCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTA                                    <  SRR4302543.832620/94‑1 (MQ=60)
                                                          GTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAA                                   <  SRR4302543.5931678/94‑1 (MQ=60)
                                                          CTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAA                                   <  SRR4302543.5931911/94‑1 (MQ=60)
                                                          CTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAA                                   <  SRR4302543.7859883/94‑1 (MQ=60)
                                                          CTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAA                                   >  SRR4302543.918467/1‑94 (MQ=60)
                                                           TGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAA                                  <  SRR4302543.682867/94‑1 (MQ=60)
                                                           TGAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAA                                  <  SRR4302543.3147744/94‑1 (MQ=60)
                                                            GAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAC                                 <  SRR4302543.2735394/94‑1 (MQ=60)
                                                            GAGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAAC                                 >  SRR4302543.5113502/1‑94 (MQ=60)
                                                             AGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACG                                <  SRR4302543.1884679/94‑1 (MQ=60)
                                                             AGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACG                                <  SRR4302543.627911/94‑1 (MQ=60)
                                                             AGCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACG                                <  SRR4302543.7256143/94‑1 (MQ=60)
                                                              GCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGA                               <  SRR4302543.5219876/94‑1 (MQ=60)
                                                              GCTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGA                               >  SRR4302543.6736494/1‑94 (MQ=60)
                                                               GTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAT                              <  SRR4302543.6144138/94‑1 (MQ=60)
                                                               CTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAATGAT                              <  SRR4302543.4127390/94‑1 (MQ=60)
                                                               CTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAT                              >  SRR4302543.7831363/1‑94 (MQ=60)
                                                               CTGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGAT                              >  SRR4302543.6638694/1‑94 (MQ=60)
                                                                TGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                             >  SRR4302543.2814076/1‑94 (MQ=60)
                                                                TGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                             <  SRR4302543.4993126/94‑1 (MQ=60)
                                                                TGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                             <  SRR4302543.28002/94‑1 (MQ=60)
                                                                TGATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATA                             >  SRR4302543.5413779/1‑94 (MQ=60)
                                                                 GATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATAC                            <  SRR4302543.6660968/94‑1 (MQ=60)
                                                                 GATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATAC                            >  SRR4302543.7883582/1‑94 (MQ=60)
                                                                  ATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACA                           <  SRR4302543.5845161/94‑1 (MQ=60)
                                                                  ATGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACA                           <  SRR4302543.5251860/94‑1 (MQ=60)
                                                                   TGCAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACAT                          <  SRR4302543.6739015/94‑1 (MQ=60)
                                                                     CAAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATAC                        <  SRR4302543.4160273/94‑1 (MQ=60)
                                                                      AAACGGCGCTAAACTGGGCGTATGGTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACA                       <  SRR4302543.286618/94‑1 (MQ=60)
                                                                      AAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACA                       <  SRR4302543.7639348/94‑1 (MQ=60)
                                                                      AAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACA                       >  SRR4302543.5109879/1‑94 (MQ=60)
                                                                      AAACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACA                       >  SRR4302543.169344/1‑94 (MQ=60)
                                                                       AACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAA                      >  SRR4302543.7937405/1‑94 (MQ=60)
                                                                       AACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAA                      <  SRR4302543.6422336/94‑1 (MQ=60)
                                                                        ACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAA                     >  SRR4302543.287089/1‑94 (MQ=60)
                                                                        ACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAA                     <  SRR4302543.5117162/94‑1 (MQ=60)
                                                                        ACGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAA                     <  SRR4302543.5982125/94‑1 (MQ=60)
                                                                         CGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAG                    <  SRR4302543.2349700/94‑1 (MQ=60)
                                                                         CGGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAG                    <  SRR4302543.5515139/94‑1 (MQ=60)
                                                                          GGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGT                   <  SRR4302543.2102/94‑1 (MQ=60)
                                                                          GGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGT                   <  SRR4302543.2740613/94‑1 (MQ=60)
                                                                          GGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGT                   <  SRR4302543.7014658/94‑1 (MQ=60)
                                                                          GGCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGG                   >  SRR4302543.3112621/1‑94 (MQ=60)
                                                                           GCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTG                  <  SRR4302543.6288629/94‑1 (MQ=60)
                                                                           GCGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTG                  <  SRR4302543.1455295/94‑1 (MQ=60)
                                                                            CGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG                 >  SRR4302543.2340162/1‑94 (MQ=60)
                                                                            CGCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGG                 <  SRR4302543.4700943/94‑1 (MQ=60)
                                                                             GCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT                >  SRR4302543.6836864/1‑94 (MQ=60)
                                                                             GCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT                <  SRR4302543.7018353/94‑1 (MQ=60)
                                                                             GCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGT                >  SRR4302543.7648495/1‑94 (MQ=60)
                                                                             GCTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGG                >  SRR4302543.658644/1‑94 (MQ=60)
                                                                             GCTAAACTGGGCATATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGGGCTTAAACGATACATACAAAGTGGT                <  SRR4302543.670679/94‑1 (MQ=60)
                                                                              CTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG               <  SRR4302543.921882/94‑1 (MQ=60)
                                                                              CTAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTG               <  SRR4302543.2373347/94‑1 (MQ=60)
                                                                               TAAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGC              >  SRR4302543.4115530/1‑94 (MQ=60)
                                                                                AAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCG             <  SRR4302543.477875/94‑1 (MQ=60)
                                                                                AAACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCG             >  SRR4302543.3553693/1‑94 (MQ=60)
                                                                                 AACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGA            >  SRR4302543.3281454/1‑94 (MQ=60)
                                                                                  ACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAA           >  SRR4302543.5250975/1‑94 (MQ=60)
                                                                                  ACTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAA           <  SRR4302543.2390336/94‑1 (MQ=60)
                                                                                   CTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAT          >  SRR4302543.2138511/1‑94 (MQ=60)
                                                                                   CTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAT          <  SRR4302543.2912423/94‑1 (MQ=60)
                                                                                   CTGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAAT          <  SRR4302543.2819198/94‑1 (MQ=60)
                                                                                    TGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATG         <  SRR4302543.7049452/94‑1 (MQ=60)
                                                                                    TGGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATG         <  SRR4302543.6544310/94‑1 (MQ=60)
                                                                                     GGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGT        <  SRR4302543.2100397/94‑1 (MQ=60)
                                                                                     GGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGT        <  SRR4302543.7911432/94‑1 (MQ=60)
                                                                                     GGGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGT        <  SRR4302543.6007195/94‑1 (MQ=60)
                                                                                      GGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA       >  SRR4302543.1959638/1‑94 (MQ=60)
                                                                                      GGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA       <  SRR4302543.2811452/94‑1 (MQ=60)
                                                                                      GGCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTA       <  SRR4302543.4475969/94‑1 (MQ=60)
                                                                                       GCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG      <  SRR4302543.884131/94‑1 (MQ=60)
                                                                                       GCGTATGCTCACGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAG      <  SRR4302543.6117799/94‑1 (MQ=60)
                                                                                           ATTGTGGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAG  <  SRR4302543.6577344/94‑1 (MQ=60)
                                                                                             |                                                                                           
CGGGAACTCAATGCGACAGATGCGCGAATTGGCCTGCTGGCTGGACGCGGTGCAGGTGCTGAGCTGATGCAAACGGCGCTAAACTGGGCGTATCGTCGCGGTAAAACAACTTTGCGGGTGGCGACCCAAATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAG  >  NC_000913/3972544‑3972728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑