Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,473 +G intergenic (+40/‑193) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,4721.G99.2% 736.6 / ‑4.2 239intergenic (+39/‑194)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base G (115/121);  minor base T (0/2);  total (116/123)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 244 total aligned reads displayed.

GCGAACTGG‑CTACAG‑CTGAAGGATTG‑ATTGCGT‑T‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGC  >  NC_000913/4008396‑4008558
                                                                                      |                                                                                       
acaaaCTGGTCTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTa                                                                                <  2:5612232/90‑1 (MQ=255)
   actggg‑CTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATggg                                                                            <  2:2080788/89‑1 (MQ=255)
   actggg‑CTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATggg                                                                            <  1:7466089/89‑1 (MQ=255)
      ggg‑CTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAg                                                                         >  1:2553413/2‑93 (MQ=255)
       gg‑CTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAATGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGt                                                                        >  1:1933297/1‑93 (MQ=255)
       gg‑CTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGt                                                                        <  2:2543954/93‑1 (MQ=255)
       gg‑CTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGt                                                                        <  2:3153759/93‑1 (MQ=255)
       gg‑CTACAGACTGAAGGATTGTATTGCGTGTG‑GTGACAGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAATGCTGCATTATGGGCatt                                                                        <  2:1049537/93‑3 (MQ=255)
        g‑CTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTg                                                                       <  2:5154834/93‑1 (MQ=255)
        g‑CTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTg                                                                       >  1:3746607/1‑93 (MQ=255)
          cTACAGCCTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTgc                                                                      <  1:5359642/93‑2 (MQ=255)
           tACAGCCTGAAGGATTGTATTGCGT‑TTGGGGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCGTTATGGGCAGTgcg                                                                     >  2:2477351/1‑91 (MQ=255)
               gccTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCgcacc                                                                 >  1:2678634/3‑93 (MQ=255)
               gccTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCgcacc                                                                 >  2:1306646/3‑93 (MQ=255)
                catGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCgcacca                                                                <  1:4499914/91‑1 (MQ=255)
                 cTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAg                                                               >  1:6403187/1‑93 (MQ=255)
                 cTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAg                                                               >  1:3818327/1‑93 (MQ=255)
                 cTGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCCCACCAg                                                               >  2:2163605/1‑93 (MQ=255)
                  tGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                              >  2:7608789/1‑93 (MQ=255)
                  tGAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                              >  2:4367900/1‑93 (MQ=255)
                  tGAAGGAGTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGc                                                              <  1:5317749/93‑1 (MQ=255)
                   gAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCg                                                             >  1:1822924/1‑93 (MQ=255)
                   gAAGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGGCGATGGC‑GGGGTACG‑GCTGCATTATGGGCAGTGCTCACCAGCg                                                             >  2:2715251/1‑93 (MQ=255)
                    aaGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGt                                                            <  2:6251007/93‑1 (MQ=255)
                     aGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                           <  1:3317964/93‑1 (MQ=255)
                     aGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                           <  1:5116906/93‑1 (MQ=255)
                     aGGATTGTATTGCGT‑TTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTc                                                           <  2:5912240/93‑1 (MQ=255)
                                    tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                             >  1:3521706/5‑93 (MQ=255)
                                    tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                             >  2:3572813/5‑93 (MQ=255)
                                    tttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAt                                             <  2:5282403/89‑1 (MQ=255)
                                     ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                            >  2:6184843/4‑93 (MQ=255)
                                     ttggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATc                                            <  2:2031600/90‑1 (MQ=255)
                                      tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                           <  1:7678185/91‑1 (MQ=255)
                                      tggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATcc                                           <  1:5272799/91‑1 (MQ=255)
                                       ggtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATccc                                          <  1:246737/92‑1 (MQ=255)
                                        gtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCg                                         >  2:7505738/1‑93 (MQ=255)
                                        gtgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCACCGTCTGAAAGACCTTCATCCCg                                         >  2:4687845/1‑93 (MQ=255)
                                         tgACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGa                                        >  2:6127203/1‑93 (MQ=255)
                                          gACGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAg                                       <  1:6093420/93‑1 (MQ=255)
                                           aCGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGc                                      <  1:778828/93‑1 (MQ=255)
                                            cGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                     >  2:4503882/1‑93 (MQ=255)
                                            cGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                     <  1:2117140/93‑1 (MQ=255)
                                              ggATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCTAGCT‑gg                                  >  1:4801420/1‑92 (MQ=255)
                                               gATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                 >  1:6484950/1‑91 (MQ=255)
                                               gATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                 >  1:1429259/1‑91 (MQ=255)
                                               gATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                 >  2:3424089/1‑91 (MQ=255)
                                               gATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                 >  1:618750/1‑91 (MQ=255)
                                               gATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gga                                 >  1:5866394/1‑91 (MQ=255)
                                                aTGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                >  2:4244497/1‑90 (MQ=255)
                                                 tGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                               >  1:7009211/1‑89 (MQ=255)
                                                 tGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                               <  2:6595280/93‑5 (MQ=255)
                                                 tGAACGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaag                               >  2:6761975/1‑89 (MQ=255)
                                                   aaCGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                              <  1:554284/93‑1 (MQ=255)
                                                   aaCGACGCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                              >  1:10696/1‑93 (MQ=255)
                                                    acgacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                             >  2:7913716/1‑93 (MQ=255)
                                                    acgacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGa                             <  2:7380003/93‑1 (MQ=255)
                                                     cgacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                            >  1:1715506/1‑93 (MQ=255)
                                                     cgacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAt                            <  1:2349428/93‑1 (MQ=255)
                                                      gacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                           <  1:7913688/93‑1 (MQ=255)
                                                      gacgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAtt                           <  1:5157161/93‑1 (MQ=255)
                                                       acgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                          >  2:4172934/1‑93 (MQ=255)
                                                        cgCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                         <  2:2382457/93‑1 (MQ=255)
                                                         gCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                        <  2:1711717/93‑1 (MQ=255)
                                                         gCCGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                        >  1:5693879/1‑93 (MQ=255)
                                                          ccGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                       <  1:5430293/93‑1 (MQ=255)
                                                          ccGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                       <  1:6146004/93‑1 (MQ=255)
                                                           cGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                      <  2:522793/93‑1 (MQ=255)
                                                           cGAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                      <  1:4919374/93‑1 (MQ=255)
                                                            gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTaat                     >  2:1550989/1‑91 (MQ=255)
                                                            gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                     >  2:7831439/1‑93 (MQ=255)
                                                            gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                     <  1:1261370/93‑1 (MQ=255)
                                                            gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                     >  1:3094186/1‑93 (MQ=255)
                                                            gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                     >  1:4371889/1‑93 (MQ=255)
                                                            gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                     >  2:3662362/1‑93 (MQ=255)
                                                            gAAATGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                     <  2:5716088/93‑1 (MQ=255)
                                                              aaTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                   <  1:1136057/93‑1 (MQ=255)
                                                              aaTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                   >  1:2926225/1‑93 (MQ=255)
                                                              aaTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                   >  1:5724855/1‑93 (MQ=255)
                                                               aTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                  >  2:3903730/1‑93 (MQ=255)
                                                               aTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                  <  2:4371888/93‑1 (MQ=255)
                                                               aTGCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                  <  2:2092876/93‑1 (MQ=255)
                                                                 gCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                <  2:5753793/93‑1 (MQ=255)
                                                                 gCTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                >  1:706945/1‑93 (MQ=255)
                                                                  cTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc               <  1:2079924/93‑1 (MQ=255)
                                                                  cTGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc               <  2:3817108/93‑1 (MQ=255)
                                                                   tGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAGGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg              >  1:6826650/1‑93 (MQ=255)
                                                                   tGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg              <  2:1402236/93‑1 (MQ=255)
                                                                   tGTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg              >  1:7566121/1‑93 (MQ=255)
                                                                    gTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga             <  1:7611442/93‑1 (MQ=255)
                                                                    gTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga             >  1:394278/1‑93 (MQ=255)
                                                                    gTCGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcga             <  2:7457093/93‑1 (MQ=255)
                                                                      cGATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacg           <  2:643937/93‑1 (MQ=255)
                                                                       gATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga          >  1:904654/1‑93 (MQ=255)
                                                                       gATGGCGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcgacga          <  1:2112639/93‑1 (MQ=255)
                                                                          ggcgggg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc       >  2:6342295/5‑93 (MQ=255)
                                                                          ggcgggg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc       <  1:7315174/89‑1 (MQ=255)
                                                                            cgggg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg     <  2:4833486/91‑1 (MQ=255)
                                                                             gggg‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt    <  1:6716493/92‑1 (MQ=255)
                                                                              ggg‑‑AAAGGCTGGATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg   <  2:5411092/93‑1 (MQ=255)
                                                                               gg‑‑AAAGGCTGCATTATATGCAGTGCGCACCAGCGTCTGAAAGACCTTAATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc  <  2:6505525/93‑1 (MQ=255)
                                                                                      |                                                                                       
GCGAACTGG‑CTACAG‑CTGAAGGATTG‑ATTGCGT‑T‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGC  >  NC_000913/4008396‑4008558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 216 total aligned reads displayed.
TGGAAGGCTGGCGAACT‑GGCTACAG‑C‑TGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GG‑‑AAA‑GCTGCATTATGGGCAGTG‑GCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA  >  NC_000913/4008386‑4008565
                                                                                                  |                                                                                              
gcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGCCGCCGAAATGCTGTCGATGGCGG‑GG‑‑AAA‑G                                                                                                <  SRR4302543.871315/76‑1 (MQ=41)
gcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGG‑GG‑‑AAA‑G                                                                                                <  SRR4302543.2594920/76‑1 (MQ=53)
gcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGG‑GG‑‑AAA‑G                                                                                                <  SRR4302543.871314/76‑1 (MQ=53)
gcggtggcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGG‑GG‑‑AAA‑G                                                                                                <  SRR4302543.613228/76‑1 (MQ=53)
 gcggtggcgaagaaactgggctacagcctgaaggattgtattgagtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGCGG‑GG‑‑AAA‑G                                                                                                <  SRR4302543.4918197/45‑1 (MQ=60)
      gcgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCA                                                                                           <  SRR4302543.1716747/82‑1 (MQ=50)
       cgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCAT                                                                                          <  SRR4302543.5551311/83‑1 (MQ=49)
       cgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCAT                                                                                          >  SRR4302543.5388980/12‑94 (MQ=49)
       cgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCAT                                                                                          >  SRR4302543.7748190/12‑94 (MQ=49)
       cgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCAT                                                                                          >  SRR4302543.5214572/12‑94 (MQ=49)
       cgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCAT                                                                                          >  SRR4302543.3305303/12‑94 (MQ=49)
       cgaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCAT                                                                                          >  SRR4302543.1245429/12‑94 (MQ=49)
        gaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATT                                                                                         >  SRR4302543.3710697/11‑94 (MQ=49)
        gaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATT                                                                                         >  SRR4302543.1625040/11‑94 (MQ=49)
        gaagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATT                                                                                         <  SRR4302543.1185623/84‑1 (MQ=49)
         aagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTA                                                                                        <  SRR4302543.7939712/85‑1 (MQ=48)
         aagaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTCTCGATGGC‑G‑GGGAAAG‑GCTGCATTA                                                                                        >  SRR4302543.3527774/10‑94 (MQ=37)
          agaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTAT                                                                                       >  SRR4302543.3454503/9‑94 (MQ=48)
          agaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTAT                                                                                       <  SRR4302543.2783882/86‑1 (MQ=48)
          agaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTAT                                                                                       <  SRR4302543.1688462/86‑1 (MQ=48)
           gaaactgaGCTACAG‑CATGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATG                                                                                      <  SRR4302543.6159531/86‑1 (MQ=48)
           gaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATG                                                                                      <  SRR4302543.1364853/87‑1 (MQ=49)
           gaaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATG                                                                                      <  SRR4302543.6245875/87‑1 (MQ=49)
            aaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGG                                                                                     <  SRR4302543.5420698/88‑1 (MQ=51)
            aaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGG                                                                                     >  SRR4302543.569508/7‑94 (MQ=51)
            aaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGG                                                                                     <  SRR4302543.6687005/88‑1 (MQ=51)
            aaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGG                                                                                     <  SRR4302543.3620951/88‑1 (MQ=51)
            aaactgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGG                                                                                     >  SRR4302543.7448256/7‑94 (MQ=51)
             AACTGGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGG                                                                                    <  SRR4302543.4375771/94‑1 (MQ=50)
              ACTGGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGC                                                                                   <  SRR4302543.7537603/94‑1 (MQ=53)
               ctgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCA                                                                                  <  SRR4302543.2182330/91‑1 (MQ=57)
                tgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAG                                                                                 >  SRR4302543.2576748/3‑94 (MQ=58)
                tgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAG                                                                                 <  SRR4302543.6223323/92‑1 (MQ=58)
                tgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAG                                                                                 <  SRR4302543.591691/92‑1 (MQ=58)
                tgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAG                                                                                 >  SRR4302543.7971944/3‑94 (MQ=58)
                tgGGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAG                                                                                 <  SRR4302543.5570002/92‑1 (MQ=58)
                G‑GGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAATGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGT                                                                                >  SRR4302543.1950682/1‑94 (MQ=48)
                G‑GGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGT                                                                                <  SRR4302543.7754888/94‑1 (MQ=60)
                G‑GGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGG                                                                                >  SRR4302543.1835845/1‑94 (MQ=57)
                  GGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG                                                                               <  SRR4302543.2070410/94‑1 (MQ=60)
                  GGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG                                                                               >  SRR4302543.3779565/1‑94 (MQ=60)
                  GGCTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG                                                                               <  SRR4302543.3875117/94‑1 (MQ=60)
                    CTACAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGcg                                                                             <  SRR4302543.5408147/94‑3 (MQ=60)
                      cagG‑C‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGcgcac                                                                          <  SRR4302543.4438151/91‑6 (MQ=60)
                       CAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGcgcac                                                                          >  SRR4302543.2417016/1‑89 (MQ=60)
                       CAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGcgcac                                                                          <  SRR4302543.5876098/94‑6 (MQ=60)
                       CAGCC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGcgcac                                                                          >  SRR4302543.407299/1‑89 (MQ=60)
                        agcC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACC                                                                        >  SRR4302543.2702915/4‑94 (MQ=60)
                        agcC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACC                                                                        <  SRR4302543.7234219/91‑1 (MQ=60)
                         gcC‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCA                                                                       <  SRR4302543.5632844/92‑1 (MQ=60)
                         C‑C‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAG                                                                      >  SRR4302543.3851997/1‑94 (MQ=60)
                         C‑C‑TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAG                                                                      >  SRR4302543.4084655/1‑94 (MQ=60)
                           caTGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAG                                                                      <  SRR4302543.4540817/92‑1 (MQ=58)
                             TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCG                                                                    >  SRR4302543.1839287/1‑94 (MQ=57)
                             TGAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCG                                                                    >  SRR4302543.1979670/1‑94 (MQ=57)
                             TGAAGGAGTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCG                                                                    <  SRR4302543.5365798/94‑1 (MQ=50)
                              GAAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGT                                                                   >  SRR4302543.2765261/1‑94 (MQ=57)
                               AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTC                                                                  >  SRR4302543.1136172/1‑94 (MQ=57)
                               AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTC                                                                  >  SRR4302543.3780665/1‑94 (MQ=57)
                               AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTC                                                                  >  SRR4302543.3780664/1‑94 (MQ=57)
                               AAGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTC                                                                  >  SRR4302543.2545441/1‑94 (MQ=57)
                               AAGGAGTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTC                                                                  >  SRR4302543.7909677/1‑94 (MQ=55)
                                AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCT                                                                 <  SRR4302543.3347908/94‑1 (MQ=57)
                                AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCT                                                                 >  SRR4302543.3579796/1‑94 (MQ=57)
                                AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCT                                                                 <  SRR4302543.5163192/94‑1 (MQ=57)
                                AGGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGAACCAGCGTCT                                                                 >  SRR4302543.2509457/1‑94 (MQ=50)
                                 GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTG                                                                <  SRR4302543.6872428/94‑1 (MQ=60)
                                 GGATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTG                                                                <  SRR4302543.533840/94‑1 (MQ=60)
                                  gattgtattgcggttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTACGGGCAGTG‑CGCACCAGCGTCTGA                                                               <  SRR4302543.1414794/78‑1 (MQ=60)
                                  GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑TGCACCAGCGTCTGA                                                               >  SRR4302543.295393/1‑94 (MQ=60)
                                  GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGA                                                               >  SRR4302543.5740662/1‑94 (MQ=60)
                                  GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGA                                                               >  SRR4302543.981170/1‑94 (MQ=60)
                                  GATTGTATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGA                                                               <  SRR4302543.7754916/94‑1 (MQ=60)
                                     tgtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAG                                                            >  SRR4302543.2322846/14‑94 (MQ=60)
                                      gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGA                                                           >  SRR4302543.6201353/13‑94 (MQ=60)
                                      gtattgcgtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGA                                                           >  SRR4302543.6201352/13‑94 (MQ=60)
                                      T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGGAAGAC                                                          >  SRR4302543.5564796/1‑94 (MQ=60)
                                      T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGAC                                                          <  SRR4302543.4513099/94‑1 (MQ=60)
                                      T‑ATTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGAC                                                          <  SRR4302543.4744975/94‑1 (MQ=60)
                                         TTGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCT                                                        >  SRR4302543.4862517/1‑94 (MQ=60)
                                          TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTT                                                       <  SRR4302543.690787/94‑1 (MQ=60)
                                          TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTT                                                       <  SRR4302543.4906814/94‑1 (MQ=60)
                                          TGCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTT                                                       <  SRR4302543.7824561/94‑1 (MQ=60)
                                           GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTC                                                      <  SRR4302543.4349461/94‑1 (MQ=60)
                                           GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTC                                                      <  SRR4302543.5948503/94‑1 (MQ=60)
                                           GCGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTC                                                      <  SRR4302543.13925/94‑1 (MQ=60)
                                            cgtttgggGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCA                                                     <  SRR4302543.2721845/86‑1 (MQ=60)
                                            CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCA                                                     >  SRR4302543.2396930/1‑94 (MQ=60)
                                            CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCA                                                     <  SRR4302543.4176419/94‑1 (MQ=60)
                                            CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCA                                                     >  SRR4302543.2366387/1‑94 (MQ=60)
                                            CGTTTGGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCA                                                     >  SRR4302543.3357637/1‑94 (MQ=60)
                                             gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                    <  SRR4302543.4838417/89‑1 (MQ=60)
                                             gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                    <  SRR4302543.427662/89‑1 (MQ=60)
                                             gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                    >  SRR4302543.5440228/6‑94 (MQ=60)
                                             gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                    <  SRR4302543.427661/89‑1 (MQ=60)
                                             gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                    >  SRR4302543.3553128/6‑94 (MQ=60)
                                             gtttgGTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                    <  SRR4302543.5142485/89‑1 (MQ=60)
                                             gttgggtggaGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCAT                                                    <  SRR4302543.5248183/84‑1 (MQ=60)
                                              TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCCCCAGCGTCTGAAAGACCTTCATCCC                                                 <  SRR4302543.295656/94‑1 (MQ=60)
                                              TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCC                                                 <  SRR4302543.7750920/94‑1 (MQ=60)
                                              TG‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCC                                                 <  SRR4302543.5320369/94‑1 (MQ=60)
                                               G‑‑GTGACTGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCG                                                <  SRR4302543.5976552/94‑1 (MQ=60)
                                               G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCG                                                <  SRR4302543.872164/94‑1 (MQ=60)
                                               G‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCG                                                <  SRR4302543.248878/94‑1 (MQ=60)
                                                  GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGA                                               >  SRR4302543.4856664/1‑94 (MQ=60)
                                                   TGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAG                                              >  SRR4302543.7402725/1‑94 (MQ=60)
                                                    GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGT                                             >  SRR4302543.1827439/1‑94 (MQ=60)
                                                    GACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGC                                             <  SRR4302543.6151166/94‑1 (MQ=60)
                                                     ACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT                                            <  SRR4302543.785671/94‑1 (MQ=60)
                                                     ACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT                                            <  SRR4302543.4992703/94‑1 (MQ=60)
                                                      CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                          >  SRR4302543.7298166/1‑94 (MQ=60)
                                                      CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                          >  SRR4302543.5929627/1‑94 (MQ=60)
                                                      CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                          <  SRR4302543.2136092/94‑1 (MQ=60)
                                                      CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                          <  SRR4302543.7302850/94‑1 (MQ=60)
                                                       GGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCTAGCT‑GG                                         >  SRR4302543.4845302/1‑94 (MQ=60)
                                                       GGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                         >  SRR4302543.6240515/1‑94 (MQ=60)
                                                       GGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                         >  SRR4302543.7606789/1‑94 (MQ=60)
                                                       GGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                         >  SRR4302543.5095229/1‑94 (MQ=60)
                                                        GGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                        >  SRR4302543.624410/1‑94 (MQ=60)
                                                        GGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                        >  SRR4302543.6546049/1‑94 (MQ=60)
                                                        GGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                        >  SRR4302543.1442188/1‑94 (MQ=60)
                                                        GGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                        >  SRR4302543.5919794/1‑94 (MQ=60)
                                                         GATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                       <  SRR4302543.7732543/94‑4 (MQ=60)
                                                          ATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                       >  SRR4302543.4824059/1‑94 (MQ=60)
                                                          ATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                       >  SRR4302543.7075981/1‑94 (MQ=60)
                                                           TGAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                      >  SRR4302543.1321156/1‑94 (MQ=60)
                                                            GAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                     >  SRR4302543.7730964/1‑94 (MQ=60)
                                                            GAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                     >  SRR4302543.10788/1‑94 (MQ=60)
                                                            GAACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                     <  SRR4302543.2909976/94‑1 (MQ=60)
                                                             AACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                    <  SRR4302543.559375/94‑1 (MQ=60)
                                                              ACGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                   >  SRR4302543.1730837/1‑94 (MQ=60)
                                                               CGACGCCGAAATGCTGTCGATGGC‑GTGG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                  <  SRR4302543.4706919/94‑1 (MQ=60)
                                                               CGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                  >  SRR4302543.6320317/1‑94 (MQ=60)
                                                               CGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                  <  SRR4302543.2370731/94‑1 (MQ=60)
                                                               CGACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                  >  SRR4302543.2649934/1‑94 (MQ=60)
                                                                GACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 <  SRR4302543.5203767/94‑1 (MQ=60)
                                                                GACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 <  SRR4302543.7988034/94‑1 (MQ=60)
                                                                GACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 <  SRR4302543.2326281/94‑1 (MQ=60)
                                                                GACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                 >  SRR4302543.541049/1‑94 (MQ=60)
                                                                 ACGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                <  SRR4302543.5302161/94‑1 (MQ=60)
                                                                  CGCCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                               >  SRR4302543.5745832/1‑94 (MQ=60)
                                                                    CCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                             <  SRR4302543.5479630/94‑1 (MQ=60)
                                                                    CCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                             <  SRR4302543.536243/94‑1 (MQ=60)
                                                                    CCGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                             <  SRR4302543.6204208/94‑1 (MQ=60)
                                                                     CGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                            >  SRR4302543.4411483/1‑94 (MQ=60)
                                                                     CGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                            <  SRR4302543.4964196/94‑1 (MQ=60)
                                                                     CGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                            >  SRR4302543.6465545/1‑94 (MQ=60)
                                                                     CGAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                            >  SRR4302543.3122017/1‑94 (MQ=60)
                                                                      GAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                           >  SRR4302543.7937687/1‑94 (MQ=60)
                                                                      GAAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                           <  SRR4302543.1272658/94‑1 (MQ=60)
                                                                       AAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                          >  SRR4302543.5777097/1‑94 (MQ=60)
                                                                       AAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                          >  SRR4302543.2952808/1‑94 (MQ=60)
                                                                       AAATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGAGTCTGAAAGACCTTCACCCCGAGCgggaagtgcttggacataa                          >  SRR4302543.5777098/1‑75 (MQ=41)
                                                                        AATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                         <  SRR4302543.152812/94‑1 (MQ=60)
                                                                        AATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                         <  SRR4302543.1146385/94‑1 (MQ=60)
                                                                        AATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                         <  SRR4302543.7101858/94‑1 (MQ=60)
                                                                         ATGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                        <  SRR4302543.5773731/94‑1 (MQ=60)
                                                                          TGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                       >  SRR4302543.713379/1‑94 (MQ=60)
                                                                          TGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                       <  SRR4302543.1322505/94‑1 (MQ=60)
                                                                          TGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                       >  SRR4302543.5191478/1‑94 (MQ=60)
                                                                          TGCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                       >  SRR4302543.2122130/1‑94 (MQ=60)
                                                                           GCTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                      >  SRR4302543.3157507/1‑94 (MQ=60)
                                                                            GTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCTCCAGCGTCTGAAAGCCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                     <  SRR4302543.2471202/94‑1 (MQ=55)
                                                                            CTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAGGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                     >  SRR4302543.6891097/1‑94 (MQ=60)
                                                                            CTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                     >  SRR4302543.7638120/1‑94 (MQ=60)
                                                                            CTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                     >  SRR4302543.5742968/1‑94 (MQ=60)
                                                                            CTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                     <  SRR4302543.2098495/94‑1 (MQ=60)
                                                                            CTGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                     <  SRR4302543.4489452/94‑1 (MQ=60)
                                                                             TGTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                    >  SRR4302543.397923/1‑94 (MQ=60)
                                                                              GTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                   <  SRR4302543.7672238/94‑1 (MQ=60)
                                                                              GTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                   >  SRR4302543.7560842/1‑94 (MQ=60)
                                                                              GTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                   <  SRR4302543.7683712/94‑1 (MQ=60)
                                                                              GTCGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                   >  SRR4302543.6728615/1‑94 (MQ=60)
                                                                                CGATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                 >  SRR4302543.912620/1‑94 (MQ=60)
                                                                                 GATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                <  SRR4302543.2131549/94‑1 (MQ=60)
                                                                                 GATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                <  SRR4302543.3149384/94‑1 (MQ=60)
                                                                                  ATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG               <  SRR4302543.686099/94‑1 (MQ=60)
                                                                                  ATGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG               >  SRR4302543.3976406/1‑94 (MQ=60)
                                                                                   TGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC              <  SRR4302543.5734891/94‑1 (MQ=60)
                                                                                   TGGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC              <  SRR4302543.1278147/94‑1 (MQ=60)
                                                                                    GGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTGCGCCA‑CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG             <  SRR4302543.6176628/94‑1 (MQ=60)
                                                                                    GGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG             <  SRR4302543.7385665/94‑1 (MQ=60)
                                                                                    GGC‑G‑GGGAAAG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG             <  SRR4302543.969787/94‑1 (MQ=60)
                                                                                        G‑GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC         >  SRR4302543.6512940/1‑94 (MQ=60)
                                                                                         cggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT           <  SRR4302543.2133712/85‑1 (MQ=60)
                                                                                         cggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT           <  SRR4302543.1258409/85‑1 (MQ=60)
                                                                                          gggtaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG          <  SRR4302543.7244095/86‑1 (MQ=60)
                                                                                          ggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG          <  SRR4302543.6779977/86‑1 (MQ=60)
                                                                                          ggggaaagGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG          <  SRR4302543.5682819/86‑1 (MQ=60)
                                                                                          GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC        <  SRR4302543.1872261/94‑1 (MQ=60)
                                                                                          GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC        >  SRR4302543.2307215/1‑94 (MQ=60)
                                                                                          GG‑‑AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGATTGGTACTAATGCCGACGACGCGGTGCC        >  SRR4302543.4127525/1‑94 (MQ=60)
                                                                                              AAAGGCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC      >  SRR4302543.7828079/1‑94 (MQ=60)
                                                                                               AG‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT    >  SRR4302543.6903147/1‑94 (MQ=60)
                                                                                                G‑GCTGCATTATGGGCAGTG‑CGCACCCGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGACGACGACGCGGTGCCGCATT   >  SRR4302543.6969584/1‑94 (MQ=60)
                                                                                                G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT   >  SRR4302543.3281012/1‑94 (MQ=60)
                                                                                                G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT   <  SRR4302543.885511/94‑1 (MQ=60)
                                                                                                G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT   >  SRR4302543.4882494/1‑94 (MQ=60)
                                                                                                G‑GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGGACTAATGCCGACGACGCGGTGCCGCATT   >  SRR4302543.3281011/1‑94 (MQ=60)
                                                                                                  GCTGCATTATGGGCAGTG‑CGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA  <  SRR4302543.6517378/94‑1 (MQ=60)
                                                                                                  |                                                                                              
TGGAAGGCTGGCGAACT‑GGCTACAG‑C‑TGAAGGATTG‑ATTGCGTT‑‑GTGACGGGATGAACGACGCCGAAATGCTGTCGATGGC‑G‑GG‑‑AAA‑GCTGCATTATGGGCAGTG‑GCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA  >  NC_000913/4008386‑4008565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑