Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,011,430	A→G	H263R (CAT→CGT)	metE →	tetrahydropteroyltriglutamate methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,011,430	0	A	G	99.7%	1097.4 / NA	350	H263R (CAT→CGT)	metE	tetrahydropteroyltriglutamate methyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (167/182); total (167/183)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 366 total aligned reads displayed.

AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG  >  NC_000913/4011340‑4011519

aGGTACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTg                                                                                         >  2:2501737/1‑93 (MQ=255)

 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        >  2:7263676/1‑93 (MQ=255)

 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        <  2:1091717/93‑1 (MQ=255)

 gggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCgtgt                                                                                        <  1:7546623/93‑1 (MQ=255)

  ggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGtt                                                                                       >  2:6069416/1‑93 (MQ=255)

  ggACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGtt                                                                                       <  2:3986828/93‑1 (MQ=255)

   gACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTg                                                                                      <  1:7679254/93‑1 (MQ=255)

    aCAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGa                                                                                     <  1:4709151/93‑1 (MQ=255)

    aCAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGa                                                                                     >  2:2394236/1‑93 (MQ=255)

    aCAGGTGAAACTGCTGCTGACCACCTATTTCGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGa                                                                                     <  2:3502444/93‑1 (MQ=255)

       ggTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCt                                                                                  >  1:1413298/1‑93 (MQ=255)

       ggTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCt                                                                                  >  2:3365971/1‑93 (MQ=255)

        gTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTc                                                                                 >  2:589966/1‑93 (MQ=255)

        gTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTc                                                                                 >  1:64214/1‑93 (MQ=255)

          gAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGt                                                                               >  1:398252/1‑93 (MQ=255)

          gAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGt                                                                               <  1:114281/93‑1 (MQ=255)

          gAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGt                                                                               <  2:3306440/93‑1 (MQ=255)

          gAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGt                                                                               <  2:5533706/93‑1 (MQ=255)

             aCTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACa                                                                            <  1:3684155/93‑1 (MQ=255)

             aCTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACa                                                                            <  2:2028866/93‑1 (MQ=255)

             aCTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACa                                                                            <  1:3029928/93‑1 (MQ=255)

             aCTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGGCTGCGTGTTGACCTCGTACa                                                                            >  1:5963205/1‑93 (MQ=255)

              ctgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAc                                                                                    >  1:333428/1‑84 (MQ=255)

              ctgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAc                                                                                    <  2:333429/84‑1 (MQ=255)

               tgctgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATg                                                                          >  2:6150769/1‑93 (MQ=255)

                  tgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCATGGTCTGCGTGTTGACCTCGTACATGGTa                                                                       >  2:5738125/1‑93 (MQ=255)

                  tgctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTa                                                                       <  1:5419104/93‑1 (MQ=255)

                   gctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTaa                                                                      <  2:378261/93‑1 (MQ=255)

                   gctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTaa                                                                      >  2:739025/1‑93 (MQ=255)

                    ctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTaaa                                                                     <  2:2221904/93‑1 (MQ=255)

                    ctgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTaaa                                                                     <  1:351531/93‑1 (MQ=255)

                     tgACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAg                                                                    >  2:4434764/1‑93 (MQ=255)

                      gACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGa                                                                   >  1:1271755/1‑93 (MQ=255)

                       accaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAt                                                                  <  2:3055386/93‑1 (MQ=255)

                        ccaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATg                                                                 <  1:7304687/93‑1 (MQ=255)

                        ccaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATg                                                                 >  1:4999374/1‑93 (MQ=255)

                        ccaccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGACTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATg                                                                 >  2:2690873/1‑93 (MQ=255)

                         caccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGa                                                                <  2:288964/93‑1 (MQ=255)

                         caccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGa                                                                <  1:5227821/93‑1 (MQ=255)

                          accTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAc                                                               <  1:6589700/93‑1 (MQ=255)

                          accTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAc                                                               >  2:794438/1‑93 (MQ=255)

                           ccTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACg                                                              >  2:4995247/1‑93 (MQ=255)

                            cTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGt                                                             <  2:834615/93‑1 (MQ=255)

                            cTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGt                                                             >  1:2804111/1‑93 (MQ=255)

                             tATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACgct                                                            <  1:4656337/93‑3 (MQ=255)

                             tATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGtt                                                            >  1:1636910/1‑93 (MQ=255)

                               ttttGAAGGCGTAACGCCAAATTTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGc                                                          >  1:5671304/1‑93 (MQ=255)

                               ttttGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGc                                                          >  1:5290001/1‑93 (MQ=255)

                               ttttGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGc                                                          <  2:5250029/93‑1 (MQ=255)

                               ttttGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGc                                                          >  1:7368499/1‑93 (MQ=255)

                               ttttGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGc                                                          >  2:7619867/1‑93 (MQ=255)

                                   gAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGaa                                                      >  1:5518304/1‑93 (MQ=255)

                                    aaGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAAc                                                     >  2:6903667/1‑93 (MQ=255)

                                     aGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACt                                                    >  1:7220064/1‑93 (MQ=255)

                                      ggCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTg                                                   <  2:3176570/93‑1 (MQ=255)

                                      ggCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTg                                                   <  2:5642168/93‑1 (MQ=255)

                                          tAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGcaca                                               >  1:7931937/1‑93 (MQ=255)

                                           aaCGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACaa                                              <  2:3892851/93‑1 (MQ=255)

                                           aaCGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACaa                                              >  1:4898605/1‑93 (MQ=255)

                                            aCGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAg                                             <  2:939154/93‑1 (MQ=255)

                                             cGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCATGGTCTGCGTGTTGCCCGAGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgc                                            <  1:2709684/93‑1 (MQ=255)

                                              gCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAgcg                                           >  1:3612130/1‑93 (MQ=255)

                                                cAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcc                                         <  2:6351800/93‑1 (MQ=255)

                                                 aaaTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcct                                        <  1:3388207/93‑1 (MQ=255)

                                                 aaaTCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcct                                        >  1:4215167/1‑93 (MQ=255)

                                                    tctcGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcctgcc                                     <  2:6515124/93‑1 (MQ=255)

                                                     ctcGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCgcctgcct                                    <  2:7213612/93‑1 (MQ=255)

                                                      tcGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCtt                                   <  1:1569352/93‑1 (MQ=255)

                                                       cGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTc                                  <  2:2483836/93‑1 (MQ=255)

                                                        gACACGATTACTGCGCTGCCTGTTCAGGGTCTGTGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCt                                 >  1:2994979/1‑93 (MQ=255)

                                                        gACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCt                                 <  1:4093667/93‑1 (MQ=255)

                                                          cacGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGa                               >  1:4139303/1‑93 (MQ=255)

                                                          cacGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGa                               <  1:4471590/93‑1 (MQ=255)

                                                           ccGAGTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAc                              <  1:6040872/92‑1 (MQ=255)

                                                             gATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTg                            >  1:705469/1‑93 (MQ=255)

                                                             gATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTg                            >  1:1028349/1‑93 (MQ=255)

                                                             gATTACTGCGCTGCCTGTTCAGGGTATGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTg                            >  2:6708497/1‑93 (MQ=255)

                                                              aTTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTgg                           >  1:3105237/1‑93 (MQ=255)

                                                               ttACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGt                          >  2:4325994/1‑93 (MQ=255)

                                                               ttACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGt                          <  1:7756078/93‑1 (MQ=255)

                                                                tACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGtt                         <  1:182418/93‑1 (MQ=255)

                                                                tACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGtt                         >  1:679692/1‑93 (MQ=255)

                                                                 aCTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTg                        >  1:2425249/1‑93 (MQ=255)

                                                                  cTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGc                       <  1:5894501/93‑1 (MQ=255)

                                                                   tGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCt                      <  2:7858393/93‑1 (MQ=255)

                                                                    gcgcTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTg                     <  2:7348023/93‑1 (MQ=255)

                                                                      gcTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTc                   <  2:6632633/93‑1 (MQ=255)

                                                                       cTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACCGGCTGCTGTCt                  >  2:7007866/1‑93 (MQ=255)

                                                                        tGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTg                 >  1:2127701/1‑93 (MQ=255)

                                                                        tGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTg                 >  2:4123407/1‑93 (MQ=255)

                                                                         gCCTGTTCAGGGTCTGCGTGTTGACCTGGTACATGGTAAAGATGACGTTGCTGAACTGCCCAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGc                <  2:5353114/93‑1 (MQ=255)

                                                                            tGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCggg             <  2:6454317/93‑1 (MQ=255)

                                                                              ttCCGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGCACTGCACACGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTc           <  1:2555030/93‑1 (MQ=255)

                                                                               tCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCt          <  1:6893891/93‑1 (MQ=255)

                                                                                cAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTg         >  1:648345/1‑93 (MQ=255)

                                                                                 aGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGa        <  1:1487469/93‑1 (MQ=255)

                                                                                  gggTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGAt       <  2:1818649/93‑1 (MQ=255)

                                                                                    gTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGCCTGATCa     >  1:5074984/1‑93 (MQ=255)

                                                                                       tGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCATAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATg  <  2:4675807/93‑1 (MQ=255)

                                                                                       tGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATg  <  1:2093698/93‑1 (MQ=255)

AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATG  >  NC_000913/4011340‑4011519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 212 total aligned reads displayed.

TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  NC_000913/4011337‑4011523

TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCG                                                                                               >  SRR4302543.4483250/1‑94 (MQ=60)

TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCG                                                                                               >  SRR4302543.3767353/1‑94 (MQ=60)

TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCG                                                                                               >  SRR4302543.5488526/1‑94 (MQ=60)

TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCG                                                                                               >  SRR4302543.6072636/1‑94 (MQ=60)

TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCG                                                                                               <  SRR4302543.743518/94‑1 (MQ=60)

TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCG                                                                                               <  SRR4302543.1409077/94‑1 (MQ=60)

 CCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGT                                                                                              <  SRR4302543.1094615/94‑1 (MQ=60)

  CAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTG                                                                                             >  SRR4302543.2506326/1‑94 (MQ=60)

  CAGGGACAGGTGAAACTGCTACTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTG                                                                                             <  SRR4302543.1258439/94‑1 (MQ=60)

  CAGGGACAGGGGAGACTGCTACTGACCACCTTTTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTG                                                                                             <  SRR4302543.1258438/94‑1 (MQ=60)

   AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGT                                                                                            >  SRR4302543.2520850/1‑94 (MQ=60)

   AGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGT                                                                                            <  SRR4302543.2347921/94‑1 (MQ=60)

    GGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTT                                                                                           <  SRR4302543.7618520/94‑1 (MQ=60)

     GGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTG                                                                                          >  SRR4302543.1484993/1‑94 (MQ=60)

     GGACAGGTGAAACTGCTGCTGACCACCTANTTTGANGGCNTAACGCCAAATCTNGACACGATTACTGCNNTNNNNGNTCAGGGTNNGNNNGNTG                                                                                          >  SRR4302543.1286836/1‑94 (MQ=60)

      GACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGA                                                                                         <  SRR4302543.7751996/94‑1 (MQ=60)

       ATAGGTGAAATTGCTGCTGAGCATATATTGTGAGGGCGTAACGCGAATTCTCGGCGCGCTTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAC                                                                                        <  SRR4302543.3120604/94‑1 (MQ=19)

       ACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAC                                                                                        <  SRR4302543.4752191/94‑1 (MQ=60)

       ACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAC                                                                                        <  SRR4302543.4752193/94‑1 (MQ=60)

       ACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGAC                                                                                        >  SRR4302543.4718439/1‑94 (MQ=60)

         AGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACACCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCT                                                                                      >  SRR4302543.492874/1‑94 (MQ=60)

         AGGTAAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCT                                                                                      >  SRR4302543.1592220/1‑94 (MQ=60)

          GGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTC                                                                                     >  SRR4302543.1528897/1‑94 (MQ=60)

          GGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTC                                                                                     >  SRR4302543.5166777/1‑94 (MQ=60)

          GGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTC                                                                                     >  SRR4302543.64783/1‑94 (MQ=60)

           GTGCACCTGCTGCTGTCCACCTGTTTTGCAGGCGTAACGCCTAATCTCGACACGATTACTGCGCTGCCTGTTCCGGGTCTGCGTGTTGACCTCG                                                                                    <  SRR4302543.7093799/94‑1 (MQ=60)

           GTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCG                                                                                    <  SRR4302543.2140555/94‑1 (MQ=60)

            TGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGT                                                                                   >  SRR4302543.3370694/1‑94 (MQ=60)

            TGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGT                                                                                   >  SRR4302543.5346239/1‑94 (MQ=60)

             GAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTA                                                                                  >  SRR4302543.4746910/1‑94 (MQ=60)

             GAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTA                                                                                  <  SRR4302543.4990906/94‑1 (MQ=60)

             GAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTA                                                                                  <  SRR4302543.115320/94‑1 (MQ=60)

             GAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTA                                                                                  >  SRR4302543.1149988/1‑94 (MQ=60)

              AAACTTCTGGTGACCACCTATTTTGAAGGCCTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAC                                                                                 <  SRR4302543.1537949/94‑1 (MQ=60)

              AAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAC                                                                                 >  SRR4302543.3224831/1‑94 (MQ=60)

              AAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTAC                                                                                 >  SRR4302543.3029940/1‑94 (MQ=60)

               AACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGGCTGCGTGTTGACCTCGTACA                                                                                >  SRR4302543.6017808/1‑94 (MQ=60)

                ACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCagagtgga                                                                               >  SRR4302543.336489/1‑86 (MQ=60)

                ACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAT                                                                               <  SRR4302543.5504801/94‑1 (MQ=60)

                ACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAT                                                                               <  SRR4302543.3716526/94‑1 (MQ=60)

                ACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACAT                                                                               <  SRR4302543.3057297/94‑1 (MQ=60)

                 CTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATG                                                                              <  SRR4302543.7420648/94‑1 (MQ=60)

                 CTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATG                                                                              >  SRR4302543.5888274/1‑94 (MQ=60)

                  TGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACGCGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGG                                                                             <  SRR4302543.6682451/94‑1 (MQ=60)

                  TGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGG                                                                             <  SRR4302543.1742416/94‑1 (MQ=60)

                   GCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGT                                                                            <  SRR4302543.7420975/94‑1 (MQ=60)

                    CTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTA                                                                           <  SRR4302543.4846004/94‑1 (MQ=60)

                     TGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAA                                                                          <  SRR4302543.5468384/94‑1 (MQ=60)

                     TGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAA                                                                          >  SRR4302543.2735104/1‑94 (MQ=60)

                      GCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAA                                                                         <  SRR4302543.5568400/94‑1 (MQ=60)

                       CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                        <  SRR4302543.7513114/94‑1 (MQ=60)

                       CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                        <  SRR4302543.354782/94‑1 (MQ=60)

                       CTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAG                                                                        >  SRR4302543.752584/1‑94 (MQ=60)

                        TGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGA                                                                       >  SRR4302543.6095903/1‑94 (MQ=60)

                        TGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGA                                                                       >  SRR4302543.3682428/1‑94 (MQ=60)

                        TGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGA                                                                       >  SRR4302543.1283114/1‑94 (MQ=60)

                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                      >  SRR4302543.598681/1‑94 (MQ=60)

                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                      >  SRR4302543.6132552/1‑94 (MQ=60)

                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                      <  SRR4302543.3488112/94‑1 (MQ=60)

                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                      <  SRR4302543.1503674/94‑1 (MQ=60)

                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                      <  SRR4302543.6802367/94‑1 (MQ=60)

                         GACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGAT                                                                      <  SRR4302543.4232582/94‑1 (MQ=60)

                          ACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATG                                                                     >  SRR4302543.5044878/1‑94 (MQ=60)

                           CCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGA                                                                    >  SRR4302543.768713/1‑94 (MQ=60)

                           CCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGA                                                                    <  SRR4302543.7375129/94‑1 (MQ=60)

                            CACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAC                                                                   <  SRR4302543.2987840/94‑1 (MQ=60)

                            CACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAC                                                                   <  SRR4302543.5274896/94‑1 (MQ=60)

                            CACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAC                                                                   <  SRR4302543.3609184/94‑1 (MQ=60)

                            CACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAC                                                                   >  SRR4302543.6598706/1‑94 (MQ=60)

                            CACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGAC                                                                   <  SRR4302543.4761677/94‑1 (MQ=60)

                             ACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACG                                                                  <  SRR4302543.3909013/94‑1 (MQ=60)

                             ACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACG                                                                  <  SRR4302543.6651784/94‑1 (MQ=60)

                              CCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGT                                                                 <  SRR4302543.4168546/94‑1 (MQ=60)

                              CCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGT                                                                 <  SRR4302543.1166292/94‑1 (MQ=60)

                              CCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGT                                                                 >  SRR4302543.2829762/1‑94 (MQ=60)

                               CTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTT                                                                <  SRR4302543.79631/94‑1 (MQ=60)

                               CTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTT                                                                >  SRR4302543.3047834/1‑94 (MQ=60)

                               CTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTT                                                                >  SRR4302543.1651517/1‑94 (MQ=60)

                               CTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTT                                                                >  SRR4302543.6486137/1‑94 (MQ=60)

                                TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                               <  SRR4302543.3566362/94‑1 (MQ=60)

                                TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                               >  SRR4302543.106226/1‑94 (MQ=60)

                                TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                               <  SRR4302543.7814493/94‑1 (MQ=60)

                                TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                               <  SRR4302543.6591407/94‑1 (MQ=60)

                                TATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGCTG                                                               <  SRR4302543.4698743/94‑1 (MQ=60)

                                CATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTG                                                               <  SRR4302543.1797350/94‑1 (MQ=60)

                                 ATTTTGAAGGCGTAACGCCAAATTTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGC                                                              >  SRR4302543.5723068/1‑94 (MQ=60)

                                 ATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGC                                                              >  SRR4302543.7439343/1‑94 (MQ=60)

                                 ATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGC                                                              >  SRR4302543.5337755/1‑94 (MQ=60)

                                  TTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCT                                                             <  SRR4302543.2764675/94‑1 (MQ=60)

                                     TGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAA                                                          >  SRR4302543.5568420/1‑94 (MQ=60)

                                      GAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAAC                                                         <  SRR4302543.5525026/94‑1 (MQ=60)

                                      GAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAAC                                                         <  SRR4302543.3471070/94‑1 (MQ=60)

                                      GAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAAC                                                         <  SRR4302543.3471071/94‑1 (MQ=60)

                                      GAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAAC                                                         <  SRR4302543.7335958/94‑1 (MQ=60)

                                       AAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACT                                                        <  SRR4302543.5413513/94‑1 (MQ=60)

                                       AAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACT                                                        >  SRR4302543.7290042/1‑94 (MQ=60)

                                       AAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACT                                                        >  SRR4302543.4373784/1‑94 (MQ=60)

                                        AGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTG                                                       >  SRR4302543.308277/1‑94 (MQ=60)

                                        AGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTG                                                       >  SRR4302543.5749653/1‑94 (MQ=60)

                                         GGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGC                                                      >  SRR4302543.6278026/1‑94 (MQ=60)

                                         GGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGC                                                      >  SRR4302543.7289861/1‑94 (MQ=60)

                                          GCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCA                                                     <  SRR4302543.6511450/94‑1 (MQ=60)

                                          GCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCA                                                     >  SRR4302543.3584435/1‑94 (MQ=60)

                                           CGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCAC                                                    <  SRR4302543.17300/94‑1 (MQ=60)

                                            GTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACA                                                   >  SRR4302543.1851252/1‑94 (MQ=60)

                                            GTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACA                                                   >  SRR4302543.8006398/1‑94 (MQ=60)

                                             TAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAA                                                  <  SRR4302543.3093778/94‑1 (MQ=60)

                                             TAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAA                                                  >  SRR4302543.4943256/1‑94 (MQ=60)

                                              AACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAG                                                 >  SRR4302543.6014887/1‑94 (MQ=60)

                                               ACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGC                                                <  SRR4302543.3126705/94‑1 (MQ=60)

                                               ACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGC                                                >  SRR4302543.4158617/1‑94 (MQ=60)

                                                CGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCATGGTCTGCGTGTTGCCCGAGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCG                                               <  SRR4302543.2734289/94‑1 (MQ=60)

                                                CGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCG                                               <  SRR4302543.4217963/94‑1 (MQ=60)

                                                CGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCG                                               <  SRR4302543.2643376/94‑1 (MQ=60)

                                                CGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCG                                               >  SRR4302543.3643979/1‑94 (MQ=60)

                                                CGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCG                                               <  SRR4302543.1383977/94‑1 (MQ=60)

                                                 GCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGC                                              >  SRR4302543.2267196/1‑94 (MQ=60)

                                                 GCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGC                                              <  SRR4302543.791177/94‑1 (MQ=60)

                                                 GCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGC                                              <  SRR4302543.2294246/94‑1 (MQ=60)

                                                   CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                            <  SRR4302543.5181534/94‑1 (MQ=60)

                                                   CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                            >  SRR4302543.2057270/1‑94 (MQ=60)

                                                   CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                            <  SRR4302543.5832372/94‑1 (MQ=60)

                                                   CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                            >  SRR4302543.4253389/1‑94 (MQ=60)

                                                   CAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGCTAAAGATGACGTTGCTGAACTGCACAAGCGCCT                                            >  SRR4302543.2728028/1‑94 (MQ=60)

                                                    AAATCTCGACACGATTGCTGTGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTG                                           <  SRR4302543.7209909/94‑1 (MQ=60)

                                                    AAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGTGCCTG                                           >  SRR4302543.1404844/1‑94 (MQ=60)

                                                    AAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTG                                           <  SRR4302543.4057746/94‑1 (MQ=60)

                                                    AAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTG                                           <  SRR4302543.3418583/94‑1 (MQ=60)

                                                    AAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTG                                           <  SRR4302543.4057741/94‑1 (MQ=60)

                                                     AATCTCGACACGATTACTGCGCTGCCTGTTCAGGGGCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGCACTGCACAAGCGCCTGC                                          >  SRR4302543.4660155/1‑94 (MQ=60)

                                                      ATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCC                                         >  SRR4302543.2971760/1‑94 (MQ=60)

                                                      ATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCC                                         >  SRR4302543.7783476/1‑94 (MQ=60)

                                                         TCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTC                                      <  SRR4302543.5709301/94‑1 (MQ=60)

                                                         TCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTC                                      <  SRR4302543.533794/94‑1 (MQ=60)

                                                         TCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTC                                      <  SRR4302543.1583364/94‑1 (MQ=60)

                                                         TCGACACGATTACTGCGATGCCTGTTCAGGGTCTGCGTGTTAACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTC                                      <  SRR4302543.7100992/94‑1 (MQ=60)

                                                          CGACACGATTACTGCGCTGCCTGTTCAGGGTCTGTGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCT                                     >  SRR4302543.3022155/1‑94 (MQ=60)

                                                           GACACGCTTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                    <  SRR4302543.115875/94‑1 (MQ=60)

                                                           GACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                    >  SRR4302543.5703129/1‑94 (MQ=60)

                                                           GACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                    <  SRR4302543.595385/94‑1 (MQ=60)

                                                           GACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                    <  SRR4302543.4130394/94‑1 (MQ=60)

                                                           GACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                    <  SRR4302543.730209/94‑1 (MQ=60)

                                                           GACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTG                                    >  SRR4302543.4958077/1‑94 (MQ=60)

                                                            ACACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGA                                   >  SRR4302543.4176676/1‑94 (MQ=60)

                                                             CACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAC                                  >  SRR4302543.6840988/1‑94 (MQ=60)

                                                             CACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGAC                                  <  SRR4302543.4512146/94‑1 (MQ=60)

                                                              ccgagTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACT                                 <  SRR4302543.6098050/89‑1 (MQ=60)

                                                              ACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACT                                 <  SRR4302543.3648234/94‑1 (MQ=60)

                                                              ACGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACT                                 <  SRR4302543.3343316/94‑1 (MQ=60)

                                                               CGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTG                                >  SRR4302543.711898/1‑94 (MQ=60)

                                                               CGATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTG                                >  SRR4302543.1037460/1‑94 (MQ=60)

                                                                GATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCATAAGCGCCTGCCTTCTGACTGG                               <  SRR4302543.5293234/94‑1 (MQ=60)

                                                                GATTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGG                               >  SRR4302543.3133168/1‑94 (MQ=60)

                                                                  TTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTT                             <  SRR4302543.7829290/94‑1 (MQ=60)

                                                                  TTACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTT                             >  SRR4302543.685947/1‑94 (MQ=60)

                                                                   TACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTG                            <  SRR4302543.183970/94‑1 (MQ=60)

                                                                   TACTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTG                            >  SRR4302543.2447141/1‑94 (MQ=60)

                                                                     CTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCT                          <  SRR4302543.3878192/94‑1 (MQ=60)

                                                                     CTGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCT                          <  SRR4302543.5948254/94‑1 (MQ=60)

                                                                      TGCGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTG                         >  SRR4302543.5102307/1‑94 (MQ=60)

                                                                        CGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTC                       <  SRR4302543.4501422/94‑1 (MQ=60)

                                                                        CGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTC                       <  SRR4302543.7662458/94‑1 (MQ=60)

                                                                        CGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTC                       >  SRR4302543.3154347/1‑94 (MQ=60)

                                                                        CGCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTC                       >  SRR4302543.6529679/1‑94 (MQ=60)

                                                                         GCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCT                      <  SRR4302543.1264077/94‑1 (MQ=60)

                                                                         GCTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCT                      <  SRR4302543.605417/94‑1 (MQ=60)

                                                                          CTGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTG                     >  SRR4302543.2146749/1‑94 (MQ=60)

                                                                           TGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAGCTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGC                    <  SRR4302543.7244546/94‑1 (MQ=60)

                                                                           TGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGC                    >  SRR4302543.3930299/1‑94 (MQ=60)

                                                                           TGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGC                    >  SRR4302543.5091804/1‑94 (MQ=60)

                                                                           TGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGC                    <  SRR4302543.1606927/94‑1 (MQ=60)

                                                                           TGCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGC                    >  SRR4302543.2894369/1‑94 (MQ=60)

                                                                            GCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCG                   <  SRR4302543.1428102/94‑1 (MQ=60)

                                                                            GCCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCG                   <  SRR4302543.2124380/94‑1 (MQ=60)

                                                                            GCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGCTGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCG                   <  SRR4302543.7116151/94‑1 (MQ=60)

                                                                             CCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGG                  <  SRR4302543.5130381/94‑1 (MQ=60)

                                                                             CCTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGG                  >  SRR4302543.7988207/1‑94 (MQ=60)

                                                                              CTGTTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGG                 >  SRR4302543.5423235/1‑94 (MQ=60)

                                                                                 TTCCGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGCACTGCACACGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCT              <  SRR4302543.2578390/94‑1 (MQ=60)

                                                                                 TTCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCT              >  SRR4302543.1495784/1‑94 (MQ=60)

                                                                                  TCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTG             >  SRR4302543.654276/1‑94 (MQ=60)

                                                                                  TCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTG             <  SRR4302543.6958693/94‑1 (MQ=60)

                                                                                  TCAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTG             >  SRR4302543.689724/1‑94 (MQ=60)

                                                                                   CAGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGA            >  SRR4302543.4064900/1‑94 (MQ=60)

                                                                                    AGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGAT           >  SRR4302543.4401765/1‑94 (MQ=60)

                                                                                    AGGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGAT           <  SRR4302543.1500852/94‑1 (MQ=60)

                                                                                     GGGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTAATC          >  SRR4302543.729618/1‑94 (MQ=60)

                                                                                      GGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCA         >  SRR4302543.6861507/1‑94 (MQ=60)

                                                                                      GGTCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGCCTGATCA         >  SRR4302543.5121039/1‑94 (MQ=60)

                                                                                        TCTGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAAT       <  SRR4302543.2487675/94‑1 (MQ=60)

                                                                                          TGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGG     >  SRR4302543.4533201/1‑94 (MQ=60)

                                                                                          TGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGG     <  SRR4302543.771861/94‑1 (MQ=60)

                                                                                          TGCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGG     <  SRR4302543.423050/94‑1 (MQ=60)

                                                                                           GCGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGT    >  SRR4302543.6196220/1‑94 (MQ=60)

                                                                                            CGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC   >  SRR4302543.7668227/1‑94 (MQ=60)

                                                                                            CGTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTC   >  SRR4302543.4615492/1‑94 (MQ=60)

                                                                                             GTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  <  SRR4302543.6599837/94‑1 (MQ=60)

                                                                                             GTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  SRR4302543.101639/1‑94 (MQ=60)

                                                                                             GTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  SRR4302543.1178467/1‑94 (MQ=60)

                                                                                             GTGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  SRR4302543.931940/1‑94 (MQ=60)

TCCAGGGACAGGTGAAACTGCTGCTGACCACCTATTTTGAAGGCGTAACGCCAAATCTCGACACGATTACTGCGCTGCCTGTTCAGGGTCTGCATGTTGACCTCGTACATGGTAAAGATGACGTTGCTGAACTGCACAAGCGCCTGCCTTCTGACTGGTTGCTGTCTGCGGGTCTGATCAATGGTCG  >  NC_000913/4011337‑4011523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`