Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,049,149 G→A G75S (GGC→AGC)  yihI → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,049,1490GA99.2% 1372.4 / ‑5.0 377G75S (GGC→AGC) yihIhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (180/194);  total (182/195)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 383 total aligned reads displayed.

CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG  >  NC_000913/4049059‑4049238
                                                                                          |                                                                                         
 gcgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCg                                                                                        <  1:2001394/93‑1 (MQ=255)
  cgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGt                                                                                       <  1:4513101/93‑1 (MQ=255)
  cgcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGt                                                                                       <  2:5112868/93‑1 (MQ=255)
   gcAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTg                                                                                      >  1:3152063/1‑93 (MQ=255)
    cAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGa                                                                                     <  1:5316050/93‑1 (MQ=255)
     aGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAc                                                                                    <  2:278957/93‑1 (MQ=255)
        gggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                                 <  2:3780219/93‑1 (MQ=255)
        gggcggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGa                                                                                 >  1:6799560/1‑93 (MQ=255)
           cggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaa                                                                              >  2:5721318/1‑93 (MQ=255)
            ggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaaa                                                                             >  1:898200/1‑93 (MQ=255)
            ggAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGaaaaa                                                                             <  1:7161031/93‑1 (MQ=255)
             gAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAg                                                                            <  2:5813414/93‑1 (MQ=255)
             gAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTAAGCGTGACTGAAAAAg                                                                            >  2:1902109/1‑93 (MQ=255)
              aaaCACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAgc                                                                           >  1:6738139/1‑92 (MQ=255)
                acacCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCa                                                                         <  2:2317853/93‑1 (MQ=255)
                acacCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCa                                                                         >  1:3563930/1‑93 (MQ=255)
                  accacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAcc                                                                       >  1:2960342/1‑93 (MQ=255)
                   ccacGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCa                                                                      <  1:2075854/93‑1 (MQ=255)
                     acGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCaaa                                                                    <  1:5435209/93‑1 (MQ=255)
                     acGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCaaa                                                                    >  2:3891120/1‑93 (MQ=255)
                     acGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCaaa                                                                    <  1:2704737/93‑1 (MQ=255)
                       gTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACa                                                                  <  1:1573035/93‑1 (MQ=255)
                       gTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACa                                                                  <  2:5940341/93‑1 (MQ=255)
                       gTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACa                                                                  >  1:5991454/1‑93 (MQ=255)
                        tCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAg                                                                 >  2:2550092/1‑93 (MQ=255)
                          aGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGca                                                               <  2:1163800/93‑1 (MQ=255)
                          aGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGca                                                               <  1:6902290/93‑1 (MQ=255)
                            gcagcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAACCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGcaca                                                             <  2:7175909/93‑1 (MQ=255)
                              agcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaaa                                                           <  1:99704/93‑1 (MQ=255)
                              agcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaaa                                                           >  2:7108965/1‑93 (MQ=255)
                              agcaAAGGCCAGAACGCACAAAAAGATCCACGCATTGGCAGGAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACaaa                                                           >  2:3938400/1‑93 (MQ=255)
                               gcaAAGTCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                          <  1:5771670/93‑1 (MQ=255)
                               gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                          >  1:7907133/1‑93 (MQ=255)
                               gcaAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAc                                                          <  2:6332813/93‑1 (MQ=255)
                                caAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAcc                                                         >  2:4433682/1‑93 (MQ=255)
                                caAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAcc                                                         <  1:6606450/93‑1 (MQ=255)
                                  aaGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGa                                                       >  2:1368856/1‑93 (MQ=255)
                                  aaGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGa                                                       <  2:6179950/93‑1 (MQ=255)
                                  aaGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGa                                                       >  1:7483199/1‑93 (MQ=255)
                                   aGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGaa                                                      <  2:3620812/93‑1 (MQ=255)
                                     gCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAACCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAaga                                                    <  2:3330791/93‑1 (MQ=255)
                                     gCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAaga                                                    <  1:3652240/93‑1 (MQ=255)
                                     gCCAGAACGCACCAAAAGATCCACGTATGGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAaga                                                    <  1:5128108/93‑1 (MQ=255)
                                      ccAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAagag                                                   <  2:6527846/93‑1 (MQ=255)
                                       cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                                  <  1:1754516/93‑1 (MQ=255)
                                       cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                                  <  1:3244389/93‑1 (MQ=255)
                                       cAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGt                                                  <  1:4855921/93‑1 (MQ=255)
                                         gAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTga                                                <  2:3526056/93‑1 (MQ=255)
                                         gAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTga                                                >  2:7394735/1‑93 (MQ=255)
                                          aaCGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTgag                                               >  2:3001725/1‑93 (MQ=255)
                                             gCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGaaa                                            >  2:5386145/1‑93 (MQ=255)
                                             gCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGaaa                                            >  1:6867669/1‑93 (MQ=255)
                                              cACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAc                                           >  2:4706943/1‑93 (MQ=255)
                                                ccAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCt                                         >  1:4442999/1‑93 (MQ=255)
                                                 cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTa                                        <  2:7497331/93‑1 (MQ=255)
                                                 cAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTa                                        >  2:2710243/1‑93 (MQ=255)
                                                  aaaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGa                                                       >  1:1214477/1‑77 (MQ=255)
                                                  aaaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAt                                       >  1:5528940/1‑93 (MQ=255)
                                                  aaaaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAt                                       >  2:4957310/1‑93 (MQ=255)
                                                    aaGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGc                                     <  2:6083680/93‑1 (MQ=255)
                                                      gATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCtt                                   >  1:220275/1‑93 (MQ=255)
                                                       aTCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCttt                                  >  2:1435785/1‑93 (MQ=255)
                                                         ccACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACTGCACAAACCGAAGAGTGAGAAACCTATGCTTTCa                                <  1:6414504/93‑1 (MQ=255)
                                                         ccACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCa                                >  2:6029916/1‑93 (MQ=255)
                                                         ccACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCa                                <  1:3828507/93‑1 (MQ=255)
                                                          cACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAc                               <  2:5259883/93‑1 (MQ=255)
                                                          cACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAc                               <  1:5619365/93‑1 (MQ=255)
                                                          cACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAc                               >  1:534033/1‑93 (MQ=255)
                                                          cACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAc                               <  2:45251/93‑1 (MQ=255)
                                                             gTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGc                            >  2:3192558/1‑93 (MQ=255)
                                                              tATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCa                           <  1:4747402/93‑1 (MQ=255)
                                                              tATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCa                           >  1:1102670/1‑93 (MQ=255)
                                                               aTTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAg                          <  2:5632790/93‑1 (MQ=255)
                                                               aTTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAg                          >  2:4224861/1‑93 (MQ=255)
                                                                ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                         <  2:829848/93‑1 (MQ=255)
                                                                ttGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAgg                         >  1:7068244/1‑93 (MQ=255)
                                                                  ggCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCg                       >  1:5028915/1‑93 (MQ=255)
                                                                    cAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGa                     >  1:1129801/1‑93 (MQ=255)
                                                                    cAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGa                     <  1:4243053/93‑1 (MQ=255)
                                                                     aGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAg                    >  2:2484154/1‑93 (MQ=255)
                                                                      gTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGt                   >  1:623127/1‑93 (MQ=255)
                                                                       tAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGtt                  <  2:4132499/93‑1 (MQ=255)
                                                                       tAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGtt                  >  2:6692535/1‑93 (MQ=255)
                                                                         aacccccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTgg                <  1:6937023/90‑1 (MQ=255)
                                                                          aaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGa               >  1:2370203/1‑93 (MQ=255)
                                                                          aaCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGa               >  2:1196860/1‑93 (MQ=255)
                                                                            ccccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAt             >  1:25245/1‑93 (MQ=255)
                                                                            ccccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTCAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAt             <  2:6871637/93‑1 (MQ=255)
                                                                             cccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAtt            >  2:5334526/1‑93 (MQ=255)
                                                                             cccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAtt            <  2:7625258/93‑1 (MQ=255)
                                                                             cccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAtt            >  2:5553844/1‑93 (MQ=255)
                                                                              ccTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTa           >  1:3338895/1‑93 (MQ=255)
                                                                               ctgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAc          >  1:1921601/4‑93 (MQ=255)
                                                                               ctgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAc          >  2:173150/4‑93 (MQ=255)
                                                                                tgttCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACt         <  2:2912394/91‑1 (MQ=255)
                                                                                    ccATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaa     >  1:2253411/1‑93 (MQ=255)
                                                                                    ccATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaa     >  2:1547977/1‑93 (MQ=255)
                                                                                    ccATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGAGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaa     >  1:2806257/1‑93 (MQ=255)
                                                                                     cATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGaaa    <  1:2656318/93‑1 (MQ=255)
                                                                                       ttGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACg  >  2:2199875/1‑93 (MQ=255)
                                                                                          |                                                                                         
CGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG  >  NC_000913/4049059‑4049238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATG  >  NC_000913/4049056‑4049242
                                                                                             |                                                                                             
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                               <  SRR4302543.2509626/94‑1 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGA                                                                                               >  SRR4302543.4502034/1‑94 (MQ=60)
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAAGCCAGGACGCACCTAAAGATCCACGGACTGTCACTAAAACCCCTGTTCCATTGA                                                                                               >  SRR4302543.6743053/1‑94 (MQ=60)
 GCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAG                                                                                              <  SRR4302543.4446818/94‑1 (MQ=60)
    GCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGT                                                                                           <  SRR4302543.2019365/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACCCCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                          <  SRR4302543.4554139/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                          <  SRR4302543.4554140/94‑1 (MQ=60)
     CGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTG                                                                                          >  SRR4302543.3180423/1‑94 (MQ=60)
       CAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGAC                                                                                        <  SRR4302543.5364081/94‑1 (MQ=60)
        AGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACT                                                                                       >  SRR4302543.6431441/1‑94 (MQ=60)
          CGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGA                                                                                     >  SRR4302543.3843353/1‑94 (MQ=60)
          CGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGA                                                                                     >  SRR4302543.6863770/1‑94 (MQ=60)
          CGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGA                                                                                     >  SRR4302543.6863769/1‑94 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                    >  SRR4302543.2312901/1‑94 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                    <  SRR4302543.2776653/94‑1 (MQ=60)
           GGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAA                                                                                    >  SRR4302543.2704480/1‑94 (MQ=60)
            GGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAA                                                                                   <  SRR4302543.3786187/94‑1 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAG                                                                                 >  SRR4302543.6961395/1‑94 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAA                                                                                 >  SRR4302543.906128/1‑94 (MQ=60)
              CGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAA                                                                                 >  SRR4302543.1225267/1‑94 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                                <  SRR4302543.1959435/94‑1 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                                <  SRR4302543.7230607/94‑1 (MQ=60)
               GGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAG                                                                                >  SRR4302543.6948545/1‑94 (MQ=60)
                GAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGC                                                                               >  SRR4302543.6801778/1‑94 (MQ=60)
                 AAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTC                                                                              >  SRR4302543.691485/1‑94 (MQ=60)
                  AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                             <  SRR4302543.7012260/94‑1 (MQ=60)
                  AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                             >  SRR4302543.3595472/1‑94 (MQ=60)
                  AACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCA                                                                             <  SRR4302543.1696257/94‑1 (MQ=60)
                   ACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCAC                                                                            <  SRR4302543.2369533/94‑1 (MQ=60)
                    CACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACC                                                                           >  SRR4302543.4941236/1‑94 (MQ=60)
                    CACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACC                                                                           >  SRR4302543.2987220/1‑94 (MQ=60)
                     ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCA                                                                          <  SRR4302543.464622/94‑1 (MQ=60)
                     ACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGGCACCA                                                                          <  SRR4302543.2414955/94‑1 (MQ=60)
                      CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                                         <  SRR4302543.5638497/94‑1 (MQ=60)
                      CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                                         <  SRR4302543.2825218/94‑1 (MQ=60)
                      CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                                         <  SRR4302543.2094395/94‑1 (MQ=60)
                      CCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAA                                                                         >  SRR4302543.2065307/1‑94 (MQ=60)
                        ACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAC                                                                       <  SRR4302543.6187917/94‑1 (MQ=60)
                        ACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAC                                                                       <  SRR4302543.112636/94‑1 (MQ=60)
                        ACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAC                                                                       <  SRR4302543.5484579/94‑1 (MQ=60)
                        ACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAAC                                                                       <  SRR4302543.2729282/94‑1 (MQ=60)
                         CGTCAGTCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                      <  SRR4302543.84159/94‑1 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                      >  SRR4302543.6046366/1‑94 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTAAGCGTGACTGAAAAAGTCACCAAACA                                                                      >  SRR4302543.5104739/1‑94 (MQ=60)
                         CGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAATAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACA                                                                      <  SRR4302543.3544665/94‑1 (MQ=60)
                          GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                     <  SRR4302543.4843672/94‑1 (MQ=60)
                          GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                     <  SRR4302543.4924376/94‑1 (MQ=60)
                          GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                     >  SRR4302543.991198/1‑94 (MQ=60)
                          GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                     <  SRR4302543.1587069/94‑1 (MQ=60)
                          GTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAATAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAG                                                                     >  SRR4302543.2616410/1‑94 (MQ=60)
                            CAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCA                                                                   <  SRR4302543.2541660/94‑1 (MQ=60)
                             AGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCAC                                                                  <  SRR4302543.5494978/94‑1 (MQ=60)
                             AGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCAC                                                                  <  SRR4302543.6967148/94‑1 (MQ=60)
                             AGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGAAC                                                                  >  SRR4302543.5860246/1‑94 (MQ=60)
                              GGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACA                                                                 <  SRR4302543.4676825/94‑1 (MQ=60)
                              GGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACA                                                                 >  SRR4302543.1557280/1‑94 (MQ=60)
                              GGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAACGTGACTNAAAAAGTCACCAAACAGCACA                                                                 >  SRR4302543.575613/1‑94 (MQ=60)
                               GCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAA                                                                <  SRR4302543.4039189/94‑1 (MQ=60)
                               GCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAA                                                                <  SRR4302543.2230444/94‑1 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                              <  SRR4302543.100627/94‑1 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                              >  SRR4302543.3617926/1‑94 (MQ=60)
                                 AGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAAC                                                              >  SRR4302543.7981434/1‑94 (MQ=60)
                                  GCAAAGTCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACC                                                             <  SRR4302543.5824217/94‑1 (MQ=60)
                                  GCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACC                                                             <  SRR4302543.5824219/94‑1 (MQ=60)
                                  GCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACC                                                             <  SRR4302543.7284425/94‑1 (MQ=60)
                                   CAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG                                                            >  SRR4302543.7105448/1‑94 (MQ=60)
                                   CAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG                                                            <  SRR4302543.964979/94‑1 (MQ=60)
                                   CAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCG                                                            <  SRR4302543.6668717/94‑1 (MQ=60)
                                    AAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGA                                                           >  SRR4302543.7554819/1‑94 (MQ=60)
                                     AAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAA                                                          >  SRR4302543.4925006/1‑94 (MQ=60)
                                      AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAG                                                         <  SRR4302543.4810155/94‑1 (MQ=60)
                                      AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAG                                                         <  SRR4302543.523700/94‑1 (MQ=60)
                                      AGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTAACCAAACAGCACAAACCGAAG                                                         >  SRR4302543.2152435/1‑94 (MQ=60)
                                       GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                                        <  SRR4302543.5408340/94‑1 (MQ=60)
                                       GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                                        >  SRR4302543.8189/1‑94 (MQ=60)
                                       GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                                        <  SRR4302543.5125870/94‑1 (MQ=60)
                                       GGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGA                                                        >  SRR4302543.6432659/1‑94 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                       <  SRR4302543.2009656/94‑1 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                       <  SRR4302543.5841561/94‑1 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                       <  SRR4302543.3684325/94‑1 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                       <  SRR4302543.2961048/94‑1 (MQ=60)
                                        GCCAGAACGCACCAAAAGATCCACGTATGGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAG                                                       <  SRR4302543.5174457/94‑1 (MQ=60)
                                         CCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGT                                                      <  SRR4302543.2225399/94‑1 (MQ=60)
                                         CCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGT                                                      >  SRR4302543.6403400/1‑94 (MQ=60)
                                         CCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGG                                                      >  SRR4302543.5687875/1‑94 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                     <  SRR4302543.2980064/94‑1 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                     <  SRR4302543.3273728/94‑1 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                     <  SRR4302543.4900200/94‑1 (MQ=60)
                                          CAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTG                                                     <  SRR4302543.1770188/94‑1 (MQ=60)
                                           AGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGA                                                    >  SRR4302543.4253787/1‑94 (MQ=60)
                                            GAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAG                                                   >  SRR4302543.1824837/1‑94 (MQ=60)
                                            GAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAG                                                   >  SRR4302543.6703099/1‑94 (MQ=60)
                                             AACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGA                                                  >  SRR4302543.2252977/1‑94 (MQ=60)
                                             AACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGA                                                  <  SRR4302543.7680612/94‑1 (MQ=60)
                                              ACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAA                                                 <  SRR4302543.5381437/94‑1 (MQ=60)
                                               CGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAA                                                >  SRR4302543.4510103/1‑94 (MQ=60)
                                               CGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAA                                                >  SRR4302543.6932359/1‑94 (MQ=60)
                                                GCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAAC                                               <  SRR4302543.6816160/94‑1 (MQ=60)
                                                 CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                              <  SRR4302543.2593054/94‑1 (MQ=60)
                                                 CACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACC                                              >  SRR4302543.192467/1‑94 (MQ=60)
                                                  ACCAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCT                                             >  SRR4302543.4483232/1‑94 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAACCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                           <  SRR4302543.3826942/94‑1 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAagaggggagatcgga                                           >  SRR4302543.1225386/1‑79 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                           >  SRR4302543.3440623/1‑94 (MQ=60)
                                                    CAAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTAT                                           >  SRR4302543.5579171/1‑94 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                          <  SRR4302543.3135687/94‑1 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                          >  SRR4302543.6976215/1‑94 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                          >  SRR4302543.2278728/1‑94 (MQ=60)
                                                     AAAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATG                                          <  SRR4302543.5635462/94‑1 (MQ=60)
                                                      AAAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGGGAGAAACCTATGC                                         >  SRR4302543.1403574/1‑94 (MQ=60)
                                                       AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCT                                        <  SRR4302543.2764093/94‑1 (MQ=60)
                                                       AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCT                                        >  SRR4302543.4346923/1‑94 (MQ=60)
                                                       AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCT                                        >  SRR4302543.2034357/1‑94 (MQ=60)
                                                       AAGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCT                                        <  SRR4302543.3328445/94‑1 (MQ=60)
                                                        AGATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTT                                       >  SRR4302543.222174/1‑94 (MQ=60)
                                                         GATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTT                                      >  SRR4302543.6574711/1‑94 (MQ=60)
                                                          ATCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTC                                     >  SRR4302543.6280027/1‑94 (MQ=60)
                                                           TCCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCA                                    <  SRR4302543.6855597/94‑1 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACTGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   <  SRR4302543.6474926/94‑1 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   <  SRR4302543.6526175/94‑1 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   <  SRR4302543.7214457/94‑1 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   >  SRR4302543.538978/1‑94 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   >  SRR4302543.3425281/1‑94 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   <  SRR4302543.2048157/94‑1 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   >  SRR4302543.2862681/1‑94 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   <  SRR4302543.3862280/94‑1 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   >  SRR4302543.3257149/1‑94 (MQ=60)
                                                            CCACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCAC                                   <  SRR4302543.3194311/94‑1 (MQ=60)
                                                             CACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACC                                  <  SRR4302543.7413770/94‑1 (MQ=60)
                                                             CACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACC                                  <  SRR4302543.5670570/94‑1 (MQ=60)
                                                             CACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACC                                  <  SRR4302543.2166305/94‑1 (MQ=60)
                                                              ACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCG                                 <  SRR4302543.5485584/94‑1 (MQ=60)
                                                              ACGTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCG                                 >  SRR4302543.987260/1‑94 (MQ=60)
                                                                GTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCA                               >  SRR4302543.1112609/1‑94 (MQ=60)
                                                                GTATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCA                               >  SRR4302543.7214575/1‑94 (MQ=60)
                                                                 TATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAG                              >  SRR4302543.6915815/1‑94 (MQ=60)
                                                                 TATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAG                              <  SRR4302543.4790791/94‑1 (MQ=60)
                                                                 TATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAG                              <  SRR4302543.1207146/94‑1 (MQ=60)
                                                                  ATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGG                             <  SRR4302543.1185376/94‑1 (MQ=60)
                                                                  ATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGG                             >  SRR4302543.5364239/1‑94 (MQ=60)
                                                                  ATTGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGG                             >  SRR4302543.7136874/1‑94 (MQ=60)
                                                                    TGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCG                           <  SRR4302543.4476670/94‑1 (MQ=60)
                                                                    TGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCG                           >  SRR4302543.5074677/1‑94 (MQ=60)
                                                                    TGGCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCG                           >  SRR4302543.5216483/1‑94 (MQ=60)
                                                                      GCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGG                         >  SRR4302543.1140052/1‑94 (MQ=60)
                                                                      GCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGA                         <  SRR4302543.3130304/94‑1 (MQ=60)
                                                                      GCAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGA                         >  SRR4302543.1140053/1‑94 (MQ=60)
                                                                       CAGTAAAACCCCTGTTGCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAG                        >  SRR4302543.7780237/1‑94 (MQ=60)
                                                                       CAGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAG                        <  SRR4302543.4281556/94‑1 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                       >  SRR4302543.628833/1‑94 (MQ=60)
                                                                        AGTAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGT                       <  SRR4302543.1726205/94‑1 (MQ=60)
                                                                          TAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTG                     <  SRR4302543.1250415/94‑1 (MQ=60)
                                                                          TAAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTG                     <  SRR4302543.4065942/94‑1 (MQ=60)
                                                                           AAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGG                    <  SRR4302543.271457/94‑1 (MQ=60)
                                                                           AAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGG                    <  SRR4302543.5223688/94‑1 (MQ=60)
                                                                           AAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGG                    >  SRR4302543.6593705/1‑94 (MQ=60)
                                                                           AAAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGG                    <  SRR4302543.3652930/94‑1 (MQ=60)
                                                                            AACCCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA                   <  SRR4302543.7002279/94‑1 (MQ=60)
                                                                            AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA                   <  SRR4302543.7002278/94‑1 (MQ=60)
                                                                            AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA                   >  SRR4302543.2391684/1‑94 (MQ=60)
                                                                            AAACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGA                   <  SRR4302543.689663/94‑1 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                 >  SRR4302543.25472/1‑94 (MQ=60)
                                                                              ACCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAAT                 <  SRR4302543.7446147/94‑1 (MQ=60)
                                                                               CCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATT                >  SRR4302543.5819763/1‑94 (MQ=60)
                                                                               CCCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATT                >  SRR4302543.4762552/1‑94 (MQ=60)
                                                                                CCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA               >  SRR4302543.6673787/1‑94 (MQ=60)
                                                                                CCCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTA               >  SRR4302543.3368973/1‑94 (MQ=60)
                                                                                 CCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAC              >  SRR4302543.6975335/1‑94 (MQ=60)
                                                                                 CCTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTAC              >  SRR4302543.1938922/1‑94 (MQ=60)
                                                                                  CTGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACT             <  SRR4302543.3345500/94‑1 (MQ=60)
                                                                                   TGTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTG            >  SRR4302543.1887631/1‑94 (MQ=60)
                                                                                    GTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGG           <  SRR4302543.5390016/94‑1 (MQ=60)
                                                                                    GTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGG           >  SRR4302543.5511693/1‑94 (MQ=60)
                                                                                    GTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGG           >  SRR4302543.221359/1‑94 (MQ=60)
                                                                                    GTTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGCGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGG           >  SRR4302543.5125832/1‑94 (MQ=60)
                                                                                     TTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGA          >  SRR4302543.5469267/1‑94 (MQ=60)
                                                                                     TTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGA          >  SRR4302543.7929981/1‑94 (MQ=60)
                                                                                     TTCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGA          >  SRR4302543.2513470/1‑94 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA         >  SRR4302543.3126152/1‑94 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA         >  SRR4302543.5631226/1‑94 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA         >  SRR4302543.2273902/1‑94 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA         <  SRR4302543.6752056/94‑1 (MQ=60)
                                                                                      TCCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGAGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAA         >  SRR4302543.2831938/1‑94 (MQ=60)
                                                                                       CCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAA        >  SRR4302543.7153149/1‑94 (MQ=60)
                                                                                       CCATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAA        >  SRR4302543.7061958/1‑94 (MQ=60)
                                                                                        CATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAC       <  SRR4302543.2680401/94‑1 (MQ=60)
                                                                                        CATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAC       >  SRR4302543.3197759/1‑94 (MQ=60)
                                                                                        CATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAAC       <  SRR4302543.4882795/94‑1 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG      >  SRR4302543.4322810/1‑94 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG      <  SRR4302543.3664700/94‑1 (MQ=60)
                                                                                         ATTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACG      <  SRR4302543.2715671/94‑1 (MQ=60)
                                                                                          TTGAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGG     <  SRR4302543.1732906/94‑1 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT   <  SRR4302543.4060237/94‑1 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT   <  SRR4302543.1041495/94‑1 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT   <  SRR4302543.3112369/94‑1 (MQ=60)
                                                                                            GAGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGAT   <  SRR4302543.7117729/94‑1 (MQ=60)
                                                                                             AGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATG  >  SRR4302543.3560321/1‑94 (MQ=60)
                                                                                             AGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATG  <  SRR4302543.2776013/94‑1 (MQ=60)
                                                                                             AGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATG  >  SRR4302543.6873217/1‑94 (MQ=60)
                                                                                             AGCGTGACTGAAAAAGGCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATG  <  SRR4302543.178955/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCCGCGCAGCGGGCGGAAACACCACGTCAGGCAGCAAAGGCCAGAACGCACCAAAAGATCCACGTATTGGCAGTAAAACCCCTATTCCATTGGGCGTGACTGAAAAAGTCACCAAACAGCACAAACCGAAGAGTGAGAAACCTATGCTTTCACCGCAGGCGGAGTTGGAATTACTGGAAACGGATG  >  NC_000913/4049056‑4049242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑