Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,477,068	+C	intergenic (‑178/‑242)	b4256 ← / → yjgN	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,477,068	1	.	C	100.0%	776.1 / NA	243	intergenic (‑178/‑242)	b4256/yjgN	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (74/169); total (74/169)

Only 100 of 257 total aligned reads displayed.

GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT  >  NC_000913/4476975‑4477153

ggTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacg                                                                                        >  1:7449772/1‑93 (MQ=255)

  tgtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgca                                                                                      >  1:3042855/1‑91 (MQ=255)

   gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcat                                                                                     >  1:273677/1‑90 (MQ=255)

   gtAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcat                                                                                     >  1:6772596/1‑90 (MQ=255)

    tAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGcgacgcatt                                                                                    <  1:7258806/93‑5 (MQ=255)

      gCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAc                                                                                  <  1:2812925/93‑1 (MQ=255)

       ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACa                                                                                 <  2:927268/93‑1 (MQ=255)

       ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACa                                                                                 <  1:757928/93‑1 (MQ=255)

       ccTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACa                                                                                 <  2:7725589/93‑1 (MQ=255)

        ccttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg                                                                                <  1:2155507/91‑1 (MQ=255)

        cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg                                                                                <  1:1579655/93‑1 (MQ=255)

        cTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAg                                                                                <  2:3932297/93‑1 (MQ=255)

         tttATCCGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAgg                                                                               <  1:5708317/93‑1 (MQ=255)

         tttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAgg                                                                               <  1:6478815/93‑1 (MQ=255)

         tttATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAgg                                                                               <  1:5068548/93‑1 (MQ=255)

           tATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTg                                                                             <  1:2361352/93‑1 (MQ=255)

             tCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAt                                                                           >  2:5984694/1‑93 (MQ=255)

              cAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAtt                                                                          >  1:7120537/1‑93 (MQ=255)

                gCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgc                                                                        <  1:1648125/93‑1 (MQ=255)

                 cGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcg                                                                       <  1:2529528/93‑1 (MQ=255)

                   gTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcggc                                                                     >  1:4409350/1‑93 (MQ=255)

                   gTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTgcggc                                                                     >  2:4540599/1‑93 (MQ=255)

                       gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCaa                                                                 <  2:385838/93‑1 (MQ=255)

                       gACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCaa                                                                 >  1:7201197/1‑93 (MQ=255)

                        aCCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt                                                                <  2:3122822/93‑1 (MQ=255)

                        aCCGTATGCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAt                                                                <  1:3012978/93‑1 (MQ=255)

                          cGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGt                                                              >  2:5482923/1‑93 (MQ=255)

                           gTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGtt                                                             <  1:4412747/93‑1 (MQ=255)

                            tATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTa                                                            <  2:3317639/93‑1 (MQ=255)

                             aTTCGGCGGATACCTGACGAATGACGCGGGCGATAG‑GAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCACTGttct                                                           >  1:4873429/1‑91 (MQ=255)

                              ttCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAtt                                                          >  2:4910471/1‑93 (MQ=255)

                              ttCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAtt                                                          >  2:5972139/1‑93 (MQ=255)

                               tCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTc                                                         <  1:3321006/93‑1 (MQ=255)

                                  gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                      <  1:1023344/93‑1 (MQ=255)

                                  gcggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATa                                                      <  2:2364603/93‑1 (MQ=255)

                                    ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt                                                    >  2:829870/1‑93 (MQ=255)

                                    ggATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGt                                                    <  2:1434170/93‑1 (MQ=255)

                                     gATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGtt                                                   >  2:4362019/1‑93 (MQ=255)

                                        aCCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTaa                                                <  2:2782753/93‑1 (MQ=255)

                                         ccTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAc                                               <  1:4237153/93‑1 (MQ=255)

                                           tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                                             <  1:2192722/93‑1 (MQ=255)

                                           tGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAccc                                             >  2:5577889/1‑93 (MQ=255)

                                            gACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCa                                            <  2:7834607/93‑1 (MQ=255)

                                             aCGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaa                                           >  1:7301883/1‑93 (MQ=255)

                                             aCGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaa                                           <  1:4188654/93‑1 (MQ=255)

                                              cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                                          <  2:63211/93‑1 (MQ=255)

                                              cGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaat                                          <  2:3428756/93‑1 (MQ=255)

                                               gAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaata                                         <  2:5796489/93‑1 (MQ=255)

                                                aaTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataa                                        >  2:4570652/1‑93 (MQ=255)

                                                 aTGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaa                                       <  2:1820930/93‑1 (MQ=255)

                                                   gACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaa                                     <  2:1214589/93‑1 (MQ=255)

                                                    aCGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaat                                    >  2:5254444/1‑93 (MQ=255)

                                                      gcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataa                                  <  2:7224446/93‑1 (MQ=255)

                                                      gcgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataa                                  <  1:6280091/93‑1 (MQ=255)

                                                       cgcgCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCaataaaaataaa                                 <  2:501007/90‑1 (MQ=255)

                                                       cgGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCcataaaaataaa                                 <  1:7784568/93‑1 (MQ=255)

                                                         ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAga                               >  1:5334190/1‑92 (MQ=255)

                                                         ggCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGt                               <  2:5891430/93‑1 (MQ=255)

                                                          gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                              <  2:2576694/93‑1 (MQ=255)

                                                          gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                              <  2:5705402/93‑1 (MQ=255)

                                                          gCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCCGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTa                              <  2:4514498/93‑1 (MQ=255)

                                                           cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCATTGTTATTCATAGTTTAACCCAATAAAAATAAAGTaa                             <  2:533965/93‑1 (MQ=255)

                                                           cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTaa                             <  2:4247072/93‑1 (MQ=255)

                                                           cGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTaa                             <  1:4631823/93‑1 (MQ=255)

                                                            gATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAt                            <  2:7525881/93‑1 (MQ=255)

                                                               tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCTAATAAAAATAAAGTAATta                          >  2:7108771/5‑93 (MQ=255)

                                                               tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          >  2:7108773/5‑93 (MQ=255)

                                                               tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          >  1:6894391/5‑93 (MQ=255)

                                                               tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  2:1310199/89‑1 (MQ=255)

                                                               tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  2:3485831/89‑1 (MQ=255)

                                                               tagcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATta                          <  1:3963159/89‑1 (MQ=255)

                                                                agcaGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATtat                         >  1:6018107/4‑93 (MQ=255)

                                                                   aGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                      <  2:256752/93‑1 (MQ=255)

                                                                   aGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACa                      <  2:1792963/93‑1 (MQ=255)

                                                                    ggATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt                     >  1:6826089/1‑93 (MQ=255)

                                                                    ggATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt                     <  2:4717397/93‑1 (MQ=255)

                                                                    ggATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt                     <  2:2669432/93‑1 (MQ=255)

                                                                    ggATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGCTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAt                     >  2:6729805/1‑93 (MQ=255)

                                                                     gATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAtt                    <  2:1568952/93‑1 (MQ=255)

                                                                     gATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAtt                    >  1:2428430/1‑93 (MQ=255)

                                                                     gATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATCATACAtt                    <  2:4938646/93‑1 (MQ=255)

                                                                      aTTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAttt                   <  1:1167020/93‑1 (MQ=255)

                                                                       ttATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTg                  >  2:4078665/1‑93 (MQ=255)

                                                                       ttATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTg                  <  2:3012163/93‑1 (MQ=255)

                                                                       ttATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTg                  >  2:7770421/1‑93 (MQ=255)

                                                                        tATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGt                 <  1:1006937/93‑1 (MQ=255)

                                                                        tATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGt                 <  1:5596630/93‑1 (MQ=255)

                                                                         aTCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGtt                <  2:4194756/93‑1 (MQ=255)

                                                                          tCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGttt               >  2:692201/1‑93 (MQ=255)

                                                                           ccTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTa              <  2:1969384/93‑1 (MQ=255)

                                                                            cTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTaa             <  2:535916/93‑1 (MQ=255)

                                                                            cTGCAGCGTCAGGCGACGCATTACAGGGGATTGCGGCGCAATGTTATTCTTAGTTTAACCCAATAAAAATAAAGAAATATAAAATTTGTTTaa             >  1:5729858/1‑93 (MQ=255)

                                                                             tGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAt            <  2:110239/93‑1 (MQ=255)

                                                                              gCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATa           <  2:4840742/93‑1 (MQ=255)

                                                                               cAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATAc          <  2:6617242/93‑1 (MQ=255)

                                                                                  cGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAc       <  2:2407087/93‑1 (MQ=255)

                                                                                   gTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCAct      >  1:1277357/1‑93 (MQ=255)

                                                                                    tCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActc     <  1:7505802/93‑1 (MQ=255)

                                                                                    tCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActc     <  1:3864747/93‑1 (MQ=255)

                                                                                      aGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCActctc   >  2:809511/1‑93 (MQ=255)

GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT  >  NC_000913/4476975‑4477153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  >  NC_000913/4476974‑4477161

CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG                                                                                                >  SRR4302543.7521162/1‑94 (MQ=60)

CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG                                                                                                <  SRR4302543.7982611/94‑1 (MQ=60)

 GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG‑C                                                                                              >  SRR4302543.621919/1‑94 (MQ=60)

 GGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACG‑C                                                                                              >  SRR4302543.762369/1‑94 (MQ=60)

  GTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGca                                                                                              >  SRR4302543.3070293/1‑92 (MQ=60)

   TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGcat                                                                                             >  SRR4302543.276084/1‑91 (MQ=60)

   TGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGcat                                                                                             >  SRR4302543.6836572/1‑91 (MQ=60)

    GTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATT                                                                                            >  SRR4302543.4661090/1‑94 (MQ=60)

     TAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTA                                                                                           <  SRR4302543.7328962/94‑1 (MQ=60)

     TAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTA                                                                                           >  SRR4302543.6697402/1‑94 (MQ=60)

      AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAC                                                                                          <  SRR4302543.460862/94‑1 (MQ=60)

      AGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTAC                                                                                          <  SRR4302543.1701112/94‑1 (MQ=60)

       GCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACA                                                                                         <  SRR4302543.2838690/94‑1 (MQ=60)

       GCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACA                                                                                         <  SRR4302543.4309339/94‑1 (MQ=60)

        CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAG                                                                                        <  SRR4302543.5770310/94‑1 (MQ=60)

        CCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAG                                                                                        <  SRR4302543.764624/94‑1 (MQ=60)

         CTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGG                                                                                       <  SRR4302543.1593754/94‑1 (MQ=60)

         CTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGG                                                                                       >  SRR4302543.606933/1‑94 (MQ=60)

         CCTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGG                                                                                       <  SRR4302543.2174814/94‑1 (MQ=60)

          TTTATCCGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                                                      <  SRR4302543.5760395/94‑1 (MQ=60)

          TTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                                                      <  SRR4302543.5114557/94‑1 (MQ=60)

          TTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGT                                                                                      <  SRR4302543.6539861/94‑1 (MQ=60)

           TTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTG                                                                                     <  SRR4302543.4451708/94‑1 (MQ=60)

            TATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGA                                                                                    <  SRR4302543.2382767/94‑1 (MQ=60)

             ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTGCAGGTGAT                                                                                   >  SRR4302543.4156836/1‑94 (MQ=60)

             ATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGAT                                                                                   <  SRR4302543.169320/94‑1 (MQ=60)

              TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATT                                                                                  >  SRR4302543.7189711/1‑94 (MQ=60)

              TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATT                                                                                  <  SRR4302543.7311175/94‑1 (MQ=60)

              TCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATT                                                                                  <  SRR4302543.7240420/94‑1 (MQ=60)

                 GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCG                                                                               <  SRR4302543.1662865/94‑1 (MQ=60)

                 GCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCG                                                                               <  SRR4302543.734275/94‑1 (MQ=60)

                  CGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGG                                                                              <  SRR4302543.2552544/94‑1 (MQ=60)

                   GGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGC                                                                             >  SRR4302543.4449316/1‑94 (MQ=60)

                   GGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGC                                                                             >  SRR4302543.2583439/1‑94 (MQ=60)

                    GTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCG                                                                            >  SRR4302543.1021693/1‑94 (MQ=60)

                      AAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCA                                                                          >  SRR4302543.5971908/1‑94 (MQ=60)

                       AGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAA                                                                         >  SRR4302543.7271014/1‑94 (MQ=60)

                        GACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAT                                                                        >  SRR4302543.1575133/1‑94 (MQ=60)

                        GACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAAT                                                                        <  SRR4302543.1909453/94‑1 (MQ=60)

                         ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                                                       >  SRR4302543.1492780/1‑94 (MQ=60)

                         ACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                                                       >  SRR4302543.7454758/1‑94 (MQ=60)

                         ACCGTATGCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATG                                                                       <  SRR4302543.3040252/94‑1 (MQ=60)

                          CCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGT                                                                      <  SRR4302543.2403407/94‑1 (MQ=60)

                            GTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTA                                                                    <  SRR4302543.4452745/94‑1 (MQ=60)

                            GTATTCGCCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCATCGTCAGGCCACGCATTACAGGTGATTGCGGCGCAATGTTA                                                                    <  SRR4302543.4452939/94‑1 (MQ=60)

                             TATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAT                                                                   <  SRR4302543.2255921/94‑1 (MQ=60)

                             TATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAT                                                                   <  SRR4302543.5716632/94‑1 (MQ=60)

                             TATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTAT                                                                   <  SRR4302543.7139136/94‑1 (MQ=60)

                             TATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGCGAGTGCGGCGCGATGTTAT                                                                   >  SRR4302543.4917853/1‑94 (MQ=60)

                             TATTCGGCGGATACCTGACGAATGACGCGGGCGATAG‑GAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCACTGTTCT                                                                   >  SRR4302543.4917852/1‑94 (MQ=60)

                              ATTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATT                                                                  <  SRR4302543.1105900/94‑1 (MQ=60)

                               TTCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTC                                                                 <  SRR4302543.746255/94‑1 (MQ=60)

                                TCGGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCA                                                                <  SRR4302543.3350967/94‑1 (MQ=60)

                                  GGCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATA                                                              <  SRR4302543.1184102/94‑1 (MQ=60)

                                   GCGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAG                                                             <  SRR4302543.1032399/94‑1 (MQ=60)

                                    CGGATACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGT                                                            >  SRR4302543.6766687/1‑94 (MQ=60)

                                         ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                                                       >  SRR4302543.2326379/1‑94 (MQ=60)

                                         ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                                                       <  SRR4302543.7993805/94‑1 (MQ=60)

                                         ACCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAAC                                                       <  SRR4302543.3849083/94‑1 (MQ=60)

                                          CCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACC                                                      <  SRR4302543.4275591/94‑1 (MQ=60)

                                          CCTGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACC                                                      <  SRR4302543.4275588/94‑1 (MQ=60)

                                            TGACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCA                                                    <  SRR4302543.2212418/94‑1 (MQ=60)

                                             GACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAA                                                   >  SRR4302543.7372310/1‑94 (MQ=60)

                                             GACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAA                                                   <  SRR4302543.7632979/94‑1 (MQ=60)

                                              ACGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAAT                                                  <  SRR4302543.4226628/94‑1 (MQ=60)

                                               CGAATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATA                                                 <  SRR4302543.3058635/94‑1 (MQ=60)

                                                 AATGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAA                                               <  SRR4302543.5443453/94‑1 (MQ=60)

                                                   TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA                                             <  SRR4302543.6926504/94‑1 (MQ=60)

                                                   TGACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAA                                             <  SRR4302543.807733/94‑1 (MQ=60)

                                                    GACGCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATTTTATTCATAGTTTAACCCAATAAAAAT                                            <  SRR4302543.7286590/94‑1 (MQ=60)

                                                       GCGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAA                                         <  SRR4302543.6339278/94‑1 (MQ=60)

                                                        CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCCATAAAAATAAAG                                        <  SRR4302543.7857957/94‑1 (MQ=60)

                                                        CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                        <  SRR4302543.527570/94‑1 (MQ=60)

                                                        CGGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAG                                        <  SRR4302543.2469655/94‑1 (MQ=60)

                                                         GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGT                                       <  SRR4302543.3751787/94‑1 (MQ=60)

                                                         GGGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGA                                       >  SRR4302543.5382429/1‑94 (MQ=60)

                                                          GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA                                      <  SRR4302543.4843569/94‑1 (MQ=60)

                                                          GGCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTA                                      <  SRR4302543.2800112/94‑1 (MQ=60)

                                                           GCGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAA                                     >  SRR4302543.288550/1‑94 (MQ=60)

                                                            CGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAT                                    <  SRR4302543.2585119/94‑1 (MQ=60)

                                                            CGATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAAT                                    <  SRR4302543.4673921/94‑1 (MQ=60)

                                                             GATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATT                                   <  SRR4302543.1060169/94‑1 (MQ=60)

                                                              ATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA                                  >  SRR4302543.6829740/1‑94 (MQ=60)

                                                              ATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA                                  <  SRR4302543.6840586/94‑1 (MQ=60)

                                                              ATAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTA                                  >  SRR4302543.6959198/1‑94 (MQ=60)

                                                               TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                                 <  SRR4302543.295608/94‑1 (MQ=60)

                                                               TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                                 <  SRR4302543.3998261/94‑1 (MQ=60)

                                                               TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                                 <  SRR4302543.6329845/94‑1 (MQ=60)

                                                               TAGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTAT                                 >  SRR4302543.6073828/1‑94 (MQ=60)

                                                                AGC‑AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATA                                >  SRR4302543.5371280/1‑94 (MQ=60)

                                                                  gcAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAC                               <  SRR4302543.2403153/92‑1 (MQ=60)

                                                                  gcAGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATAC                               <  SRR4302543.7557985/92‑1 (MQ=60)

                                                                    AGGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACAT                             >  SRR4302543.6890532/1‑94 (MQ=60)

                                                                     GGATTATCCTGCAGCGTCAGTCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATT                            >  SRR4302543.6896444/1‑94 (MQ=60)

                                                                     GGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATT                            >  SRR4302543.7241320/1‑94 (MQ=60)

                                                                     GGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATT                            <  SRR4302543.3979783/94‑1 (MQ=60)

                                                                     GGATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATT                            >  SRR4302543.2450345/1‑94 (MQ=60)

                                                                      GATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTT                           <  SRR4302543.4735629/94‑1 (MQ=60)

                                                                       ATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTG                          <  SRR4302543.1177624/94‑1 (MQ=60)

                                                                       ATTATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTG                          >  SRR4302543.2189378/1‑94 (MQ=60)

                                                                         TATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                        <  SRR4302543.1015844/94‑1 (MQ=60)

                                                                         TATCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTT                        <  SRR4302543.5647616/94‑1 (MQ=60)

                                                                           TCCTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTA                      <  SRR4302543.4115229/94‑1 (MQ=60)

                                                                            CCTGCAGCGTCAGGCGACGCATTACAGGGGATTGCGGCGCAATGTTATTCTTAGTTTAACCCAATAAAAATAAAGaaatataaaatttgtttaa                     >  SRR4302543.5782141/1‑75 (MQ=60)

                                                                             CTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAT                    <  SRR4302543.3161901/94‑1 (MQ=60)

                                                                             CTGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAAT                    >  SRR4302543.7838278/1‑94 (MQ=60)

                                                                              TGCAGCGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATA                   >  SRR4302543.3242934/1‑94 (MQ=60)

                                                                                   CGTCAGGCGACGCATTACCGGTGCTTGCGGCGCCATGTTATTCATAGTTTAACCCActaaacatcaagtccttgtcactttttttcaaccacat              >  SRR4302543.1288752/1‑56 (MQ=33)

                                                                                   CGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT              >  SRR4302543.1288753/1‑94 (MQ=60)

                                                                                   CGTCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACT              <  SRR4302543.570247/94‑1 (MQ=60)

                                                                                     TCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT            <  SRR4302543.3898885/94‑1 (MQ=60)

                                                                                     TCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT            <  SRR4302543.4400713/94‑1 (MQ=60)

                                                                                     TCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT            <  SRR4302543.160924/94‑1 (MQ=60)

                                                                                     TCAGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCT            <  SRR4302543.7577546/94‑1 (MQ=60)

                                                                                       AGGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCT          >  SRR4302543.6472887/1‑94 (MQ=60)

                                                                                        GGCGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTT         <  SRR4302543.6855057/94‑1 (MQ=60)

                                                                                          CGACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCT       <  SRR4302543.5258224/94‑1 (MQ=60)

                                                                                           GACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTT      <  SRR4302543.2721995/94‑1 (MQ=60)

                                                                                            ACGCATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTA     >  SRR4302543.4583549/1‑94 (MQ=60)

                                                                                              C‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  >  SRR4302543.1387339/1‑94 (MQ=60)

                                                                                              C‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  <  SRR4302543.379591/94‑1 (MQ=60)

                                                                                              C‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  >  SRR4302543.4008268/1‑94 (MQ=60)

                                                                                              C‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  >  SRR4302543.106897/1‑94 (MQ=60)

                                                                                              C‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  <  SRR4302543.7267926/94‑1 (MQ=60)

CGGTGTAGCCTTTATCAGCGGTAAGACCGTATTCGGCGGATACCTGACGAATGACGCGGGCGATAGCGAGGATTATCCTGCAGCGTCAGGCGACG‑ATTACAGGTGATTGCGGCGCAATGTTATTCATAGTTTAACCCAATAAAAATAAAGTAATTATACATTTGTTTAATACCACTCTCTTCTTACGT  >  NC_000913/4476974‑4477161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`