Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,477,321 Δ1 bp coding (12/1182 nt) yjgN → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,477,3210A.99.6% 1226.1 / ‑3.9 270coding (12/1182 nt)yjgNhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (162/107);  minor base C (1/0);  total (163/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408
                                                                                         |                                                                                       
 aCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCg                                                                                   >  2:1716895/1‑93 (MQ=255)
   aTTTTACTGTCTGTATATATAAGTTATATCAATGTATTTTTTAA‑TTCAGGGAATTTTTATGTCTCAAGTTATTAATGAAATGGAT‑GTTCCGTc                                                                                 <  1:4941390/93‑1 (MQ=255)
    ttttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTcc                                                                                >  2:5526953/1‑93 (MQ=255)
    ttttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTcc                                                                                >  2:6824829/1‑93 (MQ=255)
       tACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCAt                                                                             >  2:4572593/1‑93 (MQ=255)
        aCTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCAtt                                                                            <  2:2428429/93‑1 (MQ=255)
         ctgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTc                                                                           >  2:3934667/1‑93 (MQ=255)
         ctgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTc                                                                           <  1:4540600/93‑1 (MQ=255)
          tgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCg                                                                          >  2:574771/1‑93 (MQ=255)
           gtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgt                                                                         >  1:3923268/1‑93 (MQ=255)
            tctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtt                                                                        >  1:755766/1‑93 (MQ=255)
            tctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtt                                                                        <  1:7868621/93‑1 (MQ=255)
            tctgtATATACAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtt                                                                        >  2:7770136/1‑93 (MQ=255)
             ctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgttt                                                                       >  1:7435548/1‑93 (MQ=255)
             ctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgttt                                                                       >  2:1295993/1‑93 (MQ=255)
             ctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgttt                                                                       >  1:3983343/1‑93 (MQ=255)
              tgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttg                                                                      >  1:7935708/1‑93 (MQ=255)
              tgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttg                                                                      >  1:1035421/1‑93 (MQ=255)
               gtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgt                                                                     >  1:4075663/1‑93 (MQ=255)
                tatatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgtt                                                                    >  1:5322092/1‑93 (MQ=255)
                 atatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgttt                                                                   <  2:5981952/93‑1 (MQ=255)
                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  <  1:4787681/93‑1 (MQ=255)
                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  >  2:7121025/1‑93 (MQ=255)
                   atataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGttttt                                                                 >  2:480533/1‑93 (MQ=255)
                      taAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAt                                                              >  1:3949019/1‑93 (MQ=255)
                      taAGATATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAt                                                              <  1:2916443/93‑1 (MQ=255)
                       aaGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATg                                                             <  2:5902034/93‑1 (MQ=255)
                       aaGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATg                                                             <  1:5507517/93‑1 (MQ=255)
                       aaGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATg                                                             >  1:7733187/1‑93 (MQ=255)
                          ttATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTa                                                          >  1:7675320/1‑93 (MQ=255)
                           tataTCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTAc                                                         >  1:2486902/1‑93 (MQ=255)
                            atatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACa                                                        >  2:6360153/1‑93 (MQ=255)
                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       >  2:187329/1‑93 (MQ=255)
                               tCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGt                                                     <  1:3586834/93‑1 (MQ=255)
                                cAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTg                                                    >  1:6770875/1‑93 (MQ=255)
                                cAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTg                                                    >  1:2329172/1‑93 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  2:5334189/93‑1 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   >  2:2108282/1‑93 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   >  2:1593915/1‑93 (MQ=255)
                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  1:409132/93‑1 (MQ=255)
                                  aTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgag                                                  >  2:4854152/1‑93 (MQ=255)
                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagg                                                 >  2:4830733/1‑92 (MQ=255)
                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 >  2:7365884/1‑93 (MQ=255)
                                     gATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagaga                                               <  1:1188112/93‑1 (MQ=255)
                                      aTTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGat                                              >  1:669006/1‑93 (MQ=255)
                                       ttttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGata                                             >  1:5175179/1‑93 (MQ=255)
                                        tttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGatat                                            <  2:3496322/93‑1 (MQ=255)
                                        tttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGatat                                            <  1:3281805/93‑1 (MQ=255)
                                         ttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtt                                           >  2:6659471/1‑93 (MQ=255)
                                         ttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtt                                           <  2:3042854/93‑1 (MQ=255)
                                         ttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtt                                           <  1:5661494/93‑1 (MQ=255)
                                          tttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAttt                                          >  2:3278961/1‑93 (MQ=255)
                                            ttaattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtttt                                         <  1:4365138/89‑1 (MQ=255)
                                            ttaattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtttt                                         >  1:3670620/5‑93 (MQ=255)
                                             taattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTc                                        <  1:1332514/90‑1 (MQ=255)
                                               attCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      >  2:1934655/2‑93 (MQ=255)
                                               attCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      <  2:6765843/92‑1 (MQ=255)
                                               attCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      >  2:4164234/2‑93 (MQ=255)
                                               attCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      <  1:3139005/92‑1 (MQ=255)
                                                ttCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTa                                     >  1:589617/1‑93 (MQ=255)
                                                   aGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAttt                                  >  2:4097049/1‑93 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 >  1:7579800/1‑93 (MQ=255)
                                                    gggAATTTTTATGGCTCAAGTTATGAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 <  2:4273365/93‑1 (MQ=255)
                                                     ggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgt                                >  1:1625613/1‑93 (MQ=255)
                                                      gAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtg                               <  2:4461227/93‑1 (MQ=255)
                                                      gAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtg                               >  2:2904538/1‑93 (MQ=255)
                                                       aaTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgt                              >  1:6009067/1‑93 (MQ=255)
                                                       aaTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgt                              >  1:899571/1‑93 (MQ=255)
                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GCTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             >  2:6585357/1‑93 (MQ=255)
                                                         tttttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtgg                            >  1:3042191/1‑93 (MQ=255)
                                                            ttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGa                         <  2:3392537/93‑1 (MQ=255)
                                                                tgCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     <  1:6946375/92‑1 (MQ=255)
                                                                 gCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgtg                    >  2:1539435/1‑93 (MQ=255)
                                                                 gCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgtg                    <  1:4133544/93‑1 (MQ=255)
                                                                  cTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATgtgt                   >  1:2681177/1‑93 (MQ=255)
                                                                  cTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCAATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATgtgt                   <  2:1026843/93‑1 (MQ=255)
                                                                    cAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgctg                 >  1:5125008/1‑93 (MQ=255)
                                                                      aGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTgttgtt               <  2:7521244/93‑1 (MQ=255)
                                                                      aGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTgttgtt               >  1:4607415/1‑93 (MQ=255)
                                                                        ttattaATGAAATTGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTaa             <  1:1020028/93‑1 (MQ=255)
                                                                        ttattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTaa             <  2:4933602/93‑1 (MQ=255)
                                                                        ttattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTaa             >  2:62685/1‑93 (MQ=255)
                                                                        ttattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTaa             <  2:1316250/93‑1 (MQ=255)
                                                                         tattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAc            <  2:2657163/93‑1 (MQ=255)
                                                                         tattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAc            >  1:2124970/1‑93 (MQ=255)
                                                                          attaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACg           >  2:851046/1‑93 (MQ=255)
                                                                          attaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACg           >  1:1139476/1‑93 (MQ=255)
                                                                           ttaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGa          <  1:4260845/93‑1 (MQ=255)
                                                                             aaTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAtt        >  2:6932651/1‑93 (MQ=255)
                                                                             aaTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAtt        >  2:5062215/1‑93 (MQ=255)
                                                                             aaTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAtt        >  2:1451258/1‑93 (MQ=255)
                                                                               tGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtat      <  2:4383640/93‑1 (MQ=255)
                                                                                gAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtata     <  1:6423626/93‑1 (MQ=255)
                                                                                 aaaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATaa    <  1:6897908/93‑1 (MQ=255)
                                                                                 aaaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATaa    >  2:7186397/1‑93 (MQ=255)
                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   <  2:5005401/93‑1 (MQ=255)
                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   <  1:7342752/93‑1 (MQ=255)
                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   >  2:7790278/1‑93 (MQ=255)
                                                                                   aTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACg  >  1:1831210/1‑93 (MQ=255)
                                                                                   aTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACg  >  2:7706400/1‑93 (MQ=255)
                                                                                         |                                                                                       
AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGT  >  NC_000913/4477227‑4477414
                                                                                              |                                                                                             
GGCAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATG                                                                                               <  SRR4302543.6793384/94‑1 (MQ=60)
  CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                            <  SRR4302543.4149999/94‑1 (MQ=60)
  CAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTT                                                                                            >  SRR4302543.2815214/1‑94 (MQ=60)
   AGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTC                                                                                           >  SRR4302543.6920896/1‑94 (MQ=60)
   AGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTC                                                                                           >  SRR4302543.7027019/1‑94 (MQ=60)
    GAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCC                                                                                          <  SRR4302543.5626503/94‑1 (MQ=60)
    GAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCC                                                                                          <  SRR4302543.5680711/94‑1 (MQ=60)
    GAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCC                                                                                          >  SRR4302543.7228104/1‑94 (MQ=60)
      ACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGT                                                                                        <  SRR4302543.5267857/94‑1 (MQ=60)
       CATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTC                                                                                       >  SRR4302543.7644717/1‑94 (MQ=60)
        ATTTTACTGTCTGTATATATAAGTTATATCAATGTATTTTTTAA‑TTCAGGGAATTTTTATGTCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCC                                                                                      <  SRR4302543.4986386/94‑1 (MQ=60)
        ATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCTGTCC                                                                                      >  SRR4302543.6956313/1‑94 (MQ=60)
        ATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCC                                                                                      <  SRR4302543.4986387/94‑1 (MQ=60)
         TTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCC                                                                                     >  SRR4302543.4799733/1‑94 (MQ=60)
             ACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTC                                                                                 <  SRR4302543.783071/94‑1 (MQ=60)
              CTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCG                                                                                <  SRR4302543.4581941/94‑1 (MQ=60)
              CTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCG                                                                                >  SRR4302543.1641758/1‑94 (MQ=60)
               TGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGT                                                                               >  SRR4302543.2590708/1‑94 (MQ=60)
               TGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGT                                                                               >  SRR4302543.3957895/1‑94 (MQ=60)
                GTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTT                                                                              >  SRR4302543.762451/1‑94 (MQ=60)
                 TCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTT                                                                             >  SRR4302543.7506829/1‑94 (MQ=60)
                 TCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTT                                                                             <  SRR4302543.7942714/94‑1 (MQ=60)
                 TCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTT                                                                             >  SRR4302543.4018677/1‑94 (MQ=60)
                 TCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTT                                                                             >  SRR4302543.4018676/1‑94 (MQ=60)
                  CTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTG                                                                            >  SRR4302543.1044618/1‑94 (MQ=60)
                  CTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTG                                                                            >  SRR4302543.2696668/1‑94 (MQ=60)
                  CTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTG                                                                            >  SRR4302543.8010196/1‑94 (MQ=60)
                   TGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGT                                                                           >  SRR4302543.4112196/1‑94 (MQ=60)
                    GTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTT                                                                          >  SRR4302543.7810618/1‑94 (MQ=60)
                    GTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTT                                                                          >  SRR4302543.5370202/1‑94 (MQ=60)
                     TATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTT                                                                         <  SRR4302543.1155515/94‑1 (MQ=60)
                      ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                        >  SRR4302543.7578815/1‑94 (MQ=60)
                      ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                        >  SRR4302543.4141756/1‑94 (MQ=60)
                      ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                        >  SRR4302543.6580099/1‑94 (MQ=60)
                      ATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTT                                                                        <  SRR4302543.2342519/94‑1 (MQ=60)
                       TATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTT                                                                       <  SRR4302543.4831440/94‑1 (MQ=60)
                        ATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTC                                                                      >  SRR4302543.3815004/1‑94 (MQ=60)
                         TATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCA                                                                     >  SRR4302543.3140615/1‑94 (MQ=60)
                         TATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCA                                                                     <  SRR4302543.6781610/94‑1 (MQ=60)
                          ATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAT                                                                    <  SRR4302543.5800742/94‑1 (MQ=60)
                          ATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAT                                                                    >  SRR4302543.3983944/1‑94 (MQ=60)
                          ATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAT                                                                    >  SRR4302543.3983943/1‑94 (MQ=60)
                           TAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATG                                                                   >  SRR4302543.7806261/1‑94 (MQ=60)
                           TAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATG                                                                   >  SRR4302543.7806263/1‑94 (MQ=60)
                           TAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATG                                                                   <  SRR4302543.6463114/94‑1 (MQ=60)
                           TAAGATATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATG                                                                   <  SRR4302543.2942941/94‑1 (MQ=60)
                            AAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGG                                                                  <  SRR4302543.5557518/94‑1 (MQ=60)
                             AGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGT                                                                 <  SRR4302543.4851288/94‑1 (MQ=60)
                             AGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGT                                                                 >  SRR4302543.4326492/1‑94 (MQ=60)
                              GTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTA                                                                >  SRR4302543.7748036/1‑94 (MQ=60)
                              GTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTA                                                                <  SRR4302543.3671153/94‑1 (MQ=60)
                               TTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTAC                                                               >  SRR4302543.2509431/1‑94 (MQ=60)
                                TATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACA                                                              >  SRR4302543.2311417/1‑94 (MQ=60)
                                TATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACA                                                              >  SRR4302543.7648896/1‑94 (MQ=60)
                                TATATCAATGGATTTTTTAA‑TTCAGGAAATTTTTATGGCTCAAGTTATTAATGAAATGGA‑CGTTCCGTCCCATTCGTTTGTTTTTCATGGTACA                                                              >  SRR4302543.7681243/1‑94 (MQ=60)
                                  TATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGG                                                            >  SRR4302543.315693/1‑94 (MQ=60)
                                  TATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGG                                                            >  SRR4302543.146310/1‑94 (MQ=60)
                                    TCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTG                                                          >  SRR4302543.2350322/1‑94 (MQ=60)
                                    TCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTG                                                          <  SRR4302543.3618510/94‑1 (MQ=60)
                                    TCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTG                                                          >  SRR4302543.6834831/1‑94 (MQ=60)
                                     TAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGA                                                         <  SRR4302543.4706938/94‑1 (MQ=60)
                                     CAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGA                                                         >  SRR4302543.5502891/1‑94 (MQ=60)
                                      AATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAG                                                        >  SRR4302543.333669/1‑94 (MQ=60)
                                      AATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAG                                                        <  SRR4302543.412912/94‑1 (MQ=60)
                                      AATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAG                                                        <  SRR4302543.412911/94‑1 (MQ=60)
                                        TGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAG                                                      <  SRR4302543.1271044/94‑1 (MQ=60)
                                         GGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGA                                                     >  SRR4302543.2915755/1‑94 (MQ=60)
                                          GATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGAT                                                    >  SRR4302543.675171/1‑94 (MQ=60)
                                          GATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGAT                                                    <  SRR4302543.5626475/94‑1 (MQ=60)
                                          GATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGAT                                                    <  SRR4302543.1198828/94‑1 (MQ=60)
                                           ATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATA                                                   >  SRR4302543.75058/1‑94 (MQ=60)
                                           ATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATA                                                   >  SRR4302543.5221905/1‑94 (MQ=60)
                                           ATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATA                                                   >  SRR4302543.5221906/1‑94 (MQ=60)
                                           ATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATA                                                   >  SRR4302543.4100616/1‑94 (MQ=60)
                                            TTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAT                                                  >  SRR4302543.4005954/1‑94 (MQ=60)
                                             TTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATT                                                 >  SRR4302543.2404595/1‑94 (MQ=60)
                                             TTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATT                                                 <  SRR4302543.3311430/94‑1 (MQ=60)
                                              TTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTT                                                <  SRR4302543.5713133/94‑1 (MQ=60)
                                               TTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTT                                               >  SRR4302543.3702847/1‑94 (MQ=60)
                                               TTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTT                                               >  SRR4302543.6619620/1‑94 (MQ=60)
                                                TTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTC                                              <  SRR4302543.4404678/94‑1 (MQ=60)
                                                TTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTC                                              >  SRR4302543.3061484/1‑94 (MQ=60)
                                                 TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                             <  SRR4302543.1344388/94‑1 (MQ=60)
                                                 TAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCT                                             <  SRR4302543.425363/94‑1 (MQ=60)
                                                   AATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTT                                            >  SRR4302543.7992074/1‑94 (MQ=60)
                                                    ATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTA                                           >  SRR4302543.595011/1‑94 (MQ=60)
                                                    ATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTA                                           <  SRR4302543.3167237/94‑1 (MQ=60)
                                                     TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAT                                          >  SRR4302543.7764262/1‑94 (MQ=60)
                                                     TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAT                                          <  SRR4302543.5139065/94‑1 (MQ=60)
                                                       CAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTT                                        >  SRR4302543.3374267/1‑94 (MQ=60)
                                                        AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                       >  SRR4302543.7651862/1‑94 (MQ=60)
                                                        AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                       >  SRR4302543.1475284/1‑94 (MQ=60)
                                                        AGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTG                                       >  SRR4302543.617644/1‑94 (MQ=60)
                                                         GGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGT                                      >  SRR4302543.1640094/1‑94 (MQ=60)
                                                          GGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTG                                     <  SRR4302543.6889255/94‑1 (MQ=60)
                                                           GAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGT                                    >  SRR4302543.6064444/1‑94 (MQ=60)
                                                           GAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGT                                    >  SRR4302543.907510/1‑94 (MQ=60)
                                                            AATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTG                                   >  SRR4302543.5704987/1‑94 (MQ=60)
                                                             ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                  <  SRR4302543.1852629/94‑1 (MQ=60)
                                                             ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                  <  SRR4302543.3893020/94‑1 (MQ=60)
                                                             ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                  >  SRR4302543.3069624/1‑94 (MQ=60)
                                                             ATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGG                                  <  SRR4302543.3639687/94‑1 (MQ=60)
                                                                   ATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATG                            >  SRR4302543.1062688/1‑94 (MQ=60)
                                                                   ATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATG                            <  SRR4302543.861202/94‑1 (MQ=60)
                                                                   ATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATG                            <  SRR4302543.5213149/94‑1 (MQ=60)
                                                                    TGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGT                           >  SRR4302543.3971437/1‑94 (MQ=60)
                                                                     TGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTG                          <  SRR4302543.7011730/94‑1 (MQ=60)
                                                                     GGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTG                          <  SRR4302543.4250075/94‑1 (MQ=60)
                                                                      GCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGT                         >  SRR4302543.6980749/1‑94 (MQ=60)
                                                                      GCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGT                         >  SRR4302543.2705475/1‑94 (MQ=60)
                                                                      GCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGT                         <  SRR4302543.4170839/94‑1 (MQ=60)
                                                                      GCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGT                         <  SRR4302543.4170841/94‑1 (MQ=60)
                                                                       CTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTT                        >  SRR4302543.988902/1‑94 (MQ=60)
                                                                        TCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTG                       >  SRR4302543.2416741/1‑94 (MQ=60)
                                                                        TCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGCTG                       >  SRR4302543.5171344/1‑94 (MQ=60)
                                                                         CAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGT                      <  SRR4302543.6964393/94‑1 (MQ=60)
                                                                          AAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGTGATATTTTCTTATTTGTGTGGTGAATGTGTTGTT                     >  SRR4302543.6648624/1‑94 (MQ=60)
                                                                          AAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTT                     <  SRR4302543.5911939/94‑1 (MQ=60)
                                                                          AAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTT                     >  SRR4302543.4649264/1‑94 (MQ=60)
                                                                           AGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTA                    >  SRR4302543.7349599/1‑94 (MQ=60)
                                                                             TTATTAATGAAATTGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAC                  <  SRR4302543.1029055/94‑1 (MQ=60)
                                                                             TTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAC                  <  SRR4302543.1029056/94‑1 (MQ=60)
                                                                             TTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAC                  >  SRR4302543.2143994/1‑94 (MQ=60)
                                                                             TTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAC                  >  SRR4302543.1683988/1‑94 (MQ=60)
                                                                             TTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAC                  <  SRR4302543.1114022/94‑1 (MQ=60)
                                                                              TATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACG                 >  SRR4302543.1149851/1‑94 (MQ=60)
                                                                              TATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACG                 >  SRR4302543.1149850/1‑94 (MQ=60)
                                                                              TATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACG                 >  SRR4302543.987/1‑94 (MQ=60)
                                                                                TTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAT               <  SRR4302543.4299545/94‑1 (MQ=60)
                                                                                TTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAT               <  SRR4302543.4299740/94‑1 (MQ=60)
                                                                                 TAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATT              >  SRR4302543.5195576/1‑94 (MQ=60)
                                                                                 TAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATT              <  SRR4302543.4612217/94‑1 (MQ=60)
                                                                                   ATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTAT            >  SRR4302543.7447845/1‑94 (MQ=60)
                                                                                    TGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATA           >  SRR4302543.7952574/1‑94 (MQ=60)
                                                                                     GAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAA          <  SRR4302543.4578130/94‑1 (MQ=60)
                                                                                     GAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAA          <  SRR4302543.6484155/94‑1 (MQ=60)
                                                                                      AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC         >  SRR4302543.3050918/1‑94 (MQ=60)
                                                                                      AAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAC         <  SRR4302543.6962737/94‑1 (MQ=60)
                                                                                       AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG        <  SRR4302543.4636113/94‑1 (MQ=60)
                                                                                       AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG        <  SRR4302543.7413393/94‑1 (MQ=60)
                                                                                       AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG        >  SRR4302543.6058800/1‑94 (MQ=60)
                                                                                       AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG        >  SRR4302543.3241615/1‑94 (MQ=60)
                                                                                       AATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG        >  SRR4302543.1847672/1‑94 (MQ=60)
                                                                                        ATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGC       >  SRR4302543.6488498/1‑94 (MQ=60)
                                                                                        ATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGC       >  SRR4302543.5024120/1‑94 (MQ=60)
                                                                                         TGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCT      <  SRR4302543.793996/94‑1 (MQ=60)
                                                                                          GGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTA     >  SRR4302543.2652729/1‑94 (MQ=60)
                                                                                          GGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTA     >  SRR4302543.4447930/1‑94 (MQ=60)
                                                                                              TGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATTTGTTGTTAACGATTATAACGCTAGGT  >  SRR4302543.3785994/1‑94 (MQ=60)
                                                                                              TGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGT  <  SRR4302543.2117031/94‑1 (MQ=60)
                                                                                              TGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGT  <  SRR4302543.2117032/94‑1 (MQ=60)
                                                                                              TGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGT  >  SRR4302543.431170/1‑94 (MQ=60)
                                                                                              |                                                                                             
GGCAGAACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGT  >  NC_000913/4477227‑4477414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑