Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,520,082 A→G H527H (CAT→CAC)  yjhG ← putative dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,0820AG99.7% 1129.8 / ‑5.4 356H527H (CAT→CAC) yjhGputative dehydratase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (161/194);  total (162/194)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

Only 100 of 362 total aligned reads displayed.

GGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGAC  >  NC_000913/4519992‑4520171
                                                                                          |                                                                                         
ggggCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTg                                                                                         >  1:390774/1‑93 (MQ=255)
 gggCCTCCGCCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGc                                                                                        <  2:5544962/93‑1 (MQ=255)
    ccTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTa                                                                                     >  2:1255021/1‑93 (MQ=255)
    ccTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTa                                                                                     >  2:3444927/1‑93 (MQ=255)
     cTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTAc                                                                                    >  1:5006022/1‑93 (MQ=255)
     cTCCGGACAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTAc                                                                                    <  2:2990953/93‑1 (MQ=255)
      tCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTAcc                                                                                   >  2:109016/1‑93 (MQ=255)
      tCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTAcc                                                                                   >  2:2238511/1‑93 (MQ=255)
       ccGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCg                                                                                  >  1:2477637/1‑93 (MQ=255)
        cGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGt                                                                                 >  1:1392780/1‑93 (MQ=255)
         ggCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTa                                                                                >  1:6512161/1‑93 (MQ=255)
           ccAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATg                                                                              >  1:3737862/1‑93 (MQ=255)
            cAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGa                                                                             <  2:3113831/93‑1 (MQ=255)
              gcgcTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACa                                                                           <  2:3831763/93‑1 (MQ=255)
                gcTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGa                                                                         <  1:762284/93‑1 (MQ=255)
                gcTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGa                                                                         <  1:4731617/93‑1 (MQ=255)
                gcTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGa                                                                         <  1:7234481/93‑1 (MQ=255)
                gcTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGa                                                                         <  2:6631337/93‑1 (MQ=255)
                gcTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGa                                                                         <  1:2769130/93‑1 (MQ=255)
                 cTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAt                                                                        >  1:4594170/1‑93 (MQ=255)
                  ttCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATg                                                                       >  2:262451/1‑93 (MQ=255)
                    cTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCt                                                                     <  2:7931821/93‑1 (MQ=255)
                     tGGCCCCAAATGGCCGATGCAAGCGCCAGTAGAAACGCCCGAGAAACGGGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCtt                                                                    <  2:5342256/93‑1 (MQ=255)
                      ggCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTc                                                                   <  1:1813412/93‑1 (MQ=255)
                       gCCCCACATGTCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCa                                                                  <  1:6334121/93‑1 (MQ=255)
                       gCCCCACATGGCCGATGCACGCGCGAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCa                                                                  <  2:7145025/93‑1 (MQ=255)
                       gCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCa                                                                  >  2:18094/1‑93 (MQ=255)
                       gCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCa                                                                  <  2:6812043/93‑1 (MQ=255)
                       gCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCa                                                                  <  1:5280064/93‑1 (MQ=255)
                        ccccACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAg                                                                 <  2:1437855/93‑1 (MQ=255)
                         cccACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAgg                                                                <  2:2669812/93‑1 (MQ=255)
                         cccACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAgg                                                                >  2:6694131/1‑93 (MQ=255)
                         ccaacaTGGCCGATGCGCGCGCCAGTAGAACCGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAgg                                                                <  1:1977933/90‑1 (MQ=255)
                          ccACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAggg                                                               >  1:2291617/1‑93 (MQ=255)
                           cacaTGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGc                                                              >  1:1666752/1‑93 (MQ=255)
                             caTGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGCCAGATGCTTCAGGGCAc                                                            >  2:6114971/1‑93 (MQ=255)
                             caTGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCAc                                                            <  1:1360042/93‑1 (MQ=255)
                             caTGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCAc                                                            >  1:1925826/1‑93 (MQ=255)
                                ggCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTgg                                                         <  1:3149606/93‑1 (MQ=255)
                                ggCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTgg                                                         <  1:6743196/93‑1 (MQ=255)
                                    gATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAAc                                                     >  2:3795299/1‑93 (MQ=255)
                                    gATGCACGCGCCAGTAGAAACGACCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTATCGTATGACAGATGCTTCAGGGCACTGGTAAc                                                     >  2:4686002/1‑93 (MQ=255)
                                     aTGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAAcc                                                    <  1:3492095/93‑1 (MQ=255)
                                     aTGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAAcc                                                    <  2:1563732/93‑1 (MQ=255)
                                         aCGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGt                                                >  2:723415/1‑93 (MQ=255)
                                          cgcgCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCa                                                                  <  2:5900038/74‑1 (MQ=255)
                                          cgcgCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTa                                               <  2:3675580/93‑1 (MQ=255)
                                          cgcgCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTa                                               <  2:44936/93‑1 (MQ=255)
                                           gcgcCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAGCGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAg                                              <  1:2915477/93‑1 (MQ=255)
                                              ccAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGtt                                           >  1:7290863/1‑93 (MQ=255)
                                              ccAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGtt                                           >  2:4367353/1‑93 (MQ=255)
                                                aGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGCCAGATGCTTCAGGGCACTGGTAACCTGGTAGGTttc                                         >  1:961773/1‑93 (MQ=255)
                                                aGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTttc                                         >  1:1043339/1‑93 (MQ=255)
                                                  tAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTttctt                                       >  1:7540983/1‑93 (MQ=255)
                                                   aGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTttcttc                                      <  1:4131429/93‑1 (MQ=255)
                                                   aGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTttcttc                                      <  2:3497338/93‑1 (MQ=255)
                                                    gAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTcc                                     <  2:4195221/93‑1 (MQ=255)
                                                    gAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTcc                                     >  2:5148495/1‑93 (MQ=255)
                                                    gAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTcc                                     >  2:2732108/1‑93 (MQ=255)
                                                      aaCGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCAt                                   >  2:3281757/1‑93 (MQ=255)
                                                            cGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGt                             >  1:6184961/1‑93 (MQ=255)
                                                            cGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGt                             <  1:2996575/93‑1 (MQ=255)
                                                            cGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGt                             >  1:3836309/1‑93 (MQ=255)
                                                            cGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGt                             <  2:6887107/93‑1 (MQ=255)
                                                              agaAAGGTGAAT‑GGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTAcc                          <  2:7055583/93‑1 (MQ=255)
                                                              agaAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTAc                           <  1:7846631/93‑1 (MQ=255)
                                                              agaAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTAc                           <  2:5472831/93‑1 (MQ=255)
                                                               gaAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTAcc                          >  1:4326055/1‑93 (MQ=255)
                                                               gaAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTAcc                          <  1:5390277/93‑1 (MQ=255)
                                                               gaAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTAcc                          >  1:5119817/1‑93 (MQ=255)
                                                                aaaCGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCt                         <  2:2817802/93‑1 (MQ=255)
                                                                 aaCGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTg                        >  1:6611242/1‑93 (MQ=255)
                                                                  aCGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGa                       <  1:2604480/93‑1 (MQ=255)
                                                                  aCGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGa                       >  2:7583330/1‑93 (MQ=255)
                                                                  aCGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGa                       >  1:5622553/1‑93 (MQ=255)
                                                                   cGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGaa                      <  1:3634253/93‑1 (MQ=255)
                                                                    gTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAg                     <  1:3672960/93‑1 (MQ=255)
                                                                    gTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAg                     <  2:5005419/93‑1 (MQ=255)
                                                                     tGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAgg                    >  1:2075222/1‑93 (MQ=255)
                                                                        aTCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTcc                 <  1:1251493/93‑1 (MQ=255)
                                                                        aTCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTcc                 >  1:6930250/1‑93 (MQ=255)
                                                                        aTCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTcc                 >  1:6223269/1‑93 (MQ=255)
                                                                         tCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCa                >  2:4810844/1‑93 (MQ=255)
                                                                         tCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCa                >  2:7481173/1‑93 (MQ=255)
                                                                         tCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCa                >  2:2517153/1‑93 (MQ=255)
                                                                          ctgTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCaa               <  2:5791456/91‑1 (MQ=255)
                                                                          cGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCaa               <  1:3435227/93‑1 (MQ=255)
                                                                          cGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCaa               <  2:2376252/93‑1 (MQ=255)
                                                                            gTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAAcc             >  1:908129/1‑92 (MQ=255)
                                                                                ttAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCaa         >  1:5444471/1‑93 (MQ=255)
                                                                                ttAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCaa         >  2:6478319/1‑93 (MQ=255)
                                                                                ttAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCaa         <  1:7366476/93‑1 (MQ=255)
                                                                                ttAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCaa         <  1:5755872/93‑1 (MQ=255)
                                                                                   aCGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCaataa      <  1:2693976/93‑1 (MQ=255)
                                                                                   aCGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCaataa      >  2:4078403/1‑93 (MQ=255)
                                                                                     gAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCCTCCCTGTACCTGAAGGTACAACGCCAATAATg    >  2:2082074/1‑93 (MQ=255)
                                                                                     gAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATACTg    >  2:4602230/1‑93 (MQ=255)
                                                                                      aaaCGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGa   >  1:4068682/1‑93 (MQ=255)
                                                                                       aaCGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGAc  <  1:6876305/93‑1 (MQ=255)
                                                                                       aaCGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGAc  <  2:3906691/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGAC  >  NC_000913/4519992‑4520171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 209 total aligned reads displayed.
GATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGA  >  NC_000913/4519989‑4520175
                                                                                             |                                                                                             
GATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACG                                                                                               >  SRR4302543.6954351/1‑94 (MQ=60)
GATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACG                                                                                               <  SRR4302543.3770127/94‑1 (MQ=60)
GATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACG                                                                                               >  SRR4302543.7739293/1‑94 (MQ=60)
GATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACG                                                                                               <  SRR4302543.1298652/94‑1 (MQ=60)
GATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAAAGAAACG                                                                                               >  SRR4302543.2404584/1‑94 (MQ=60)
 ATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGT                                                                                              >  SRR4302543.1101522/1‑94 (MQ=60)
 ATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGT                                                                                              <  SRR4302543.3190352/94‑1 (MQ=60)
 ATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGT                                                                                              >  SRR4302543.5038572/1‑94 (MQ=60)
  TGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTG                                                                                             <  SRR4302543.7959671/94‑1 (MQ=60)
  TGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTG                                                                                             >  SRR4302543.394381/1‑94 (MQ=60)
  TGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTG                                                                                             >  SRR4302543.6360012/1‑94 (MQ=60)
   GGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGC                                                                                            <  SRR4302543.3888025/94‑1 (MQ=60)
   GGGGCCCCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGC                                                                                            <  SRR4302543.5527531/94‑1 (MQ=60)
    GGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCT                                                                                           <  SRR4302543.5487919/94‑1 (MQ=60)
     GGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTT                                                                                          <  SRR4302543.2930002/94‑1 (MQ=60)
      GCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGACACGTGCTTA                                                                                         >  SRR4302543.7180828/1‑94 (MQ=60)
      GCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTA                                                                                         >  SRR4302543.7180827/1‑94 (MQ=60)
       CCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTAC                                                                                        >  SRR4302543.5051587/1‑94 (MQ=60)
       CCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTAC                                                                                        >  SRR4302543.6511533/1‑94 (MQ=60)
       CCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTAC                                                                                        >  SRR4302543.647063/1‑94 (MQ=60)
       CCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTAC                                                                                        >  SRR4302543.3867307/1‑94 (MQ=60)
       CCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATAGGTGATTAACGAAACGTGCTTAC                                                                                        <  SRR4302543.1400634/94‑1 (MQ=60)
         TCCGGCCAGCGCTTGTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCG                                                                                      <  SRR4302543.1658298/94‑1 (MQ=60)
         TCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCG                                                                                      >  SRR4302543.2500045/1‑94 (MQ=60)
          CCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGT                                                                                     >  SRR4302543.1405321/1‑94 (MQ=60)
           CGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTA                                                                                    >  SRR4302543.6573501/1‑94 (MQ=60)
             gccaggcgttcctgccccaactggcccgtggcCGCGCCAGTAGGAACGCCCGAAGAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATG                                                                                  <  SRR4302543.3732/62‑1 (MQ=56)
             GCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATG                                                                                  >  SRR4302543.3770744/1‑94 (MQ=60)
              CCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGA                                                                                 >  SRR4302543.6974900/1‑94 (MQ=60)
               CAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGAC                                                                                >  SRR4302543.91864/1‑94 (MQ=60)
                AGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACA                                                                               <  SRR4302543.3780112/94‑1 (MQ=60)
                AGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACA                                                                               >  SRR4302543.278957/1‑94 (MQ=60)
                  CGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGA                                                                             >  SRR4302543.3712499/1‑94 (MQ=60)
                   ggcgtccgagatcggtctcggcattcctgctgaaccgctcttccgatctccactctATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            <  SRR4302543.2923146/38‑1 (MQ=60)
                   GCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            <  SRR4302543.2581077/94‑1 (MQ=60)
                   GCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            >  SRR4302543.4635894/1‑94 (MQ=60)
                   GCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            <  SRR4302543.2794329/94‑1 (MQ=60)
                   GCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            <  SRR4302543.7304535/94‑1 (MQ=60)
                   GCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            <  SRR4302543.4774844/94‑1 (MQ=60)
                   GCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            <  SRR4302543.769003/94‑1 (MQ=60)
                   GCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            <  SRR4302543.4266738/94‑1 (MQ=60)
                   GCTTCTGCCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            <  SRR4302543.7558030/94‑1 (MQ=60)
                   GCTGCTGGCCCCACAGGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAT                                                                            <  SRR4302543.2949699/94‑1 (MQ=60)
                    CTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATG                                                                           <  SRR4302543.3020285/94‑1 (MQ=60)
                    CTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATG                                                                           <  SRR4302543.3176348/94‑1 (MQ=60)
                    CTTCTGACCCCACATGGCCGATGCACGCGCCAGTAGAATCGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATG                                                                           <  SRR4302543.3020287/94‑1 (MQ=60)
                      TCTGGCCCCACATGGCCGATGCACGCGCTAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCT                                                                         <  SRR4302543.4174268/94‑1 (MQ=60)
                       CTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTT                                                                        >  SRR4302543.4114778/1‑94 (MQ=60)
                       CTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTT                                                                        >  SRR4302543.1233623/1‑94 (MQ=60)
                       CTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACCACCGAGAAACGTGCATCGGGGATTAACGCAACGTGCTTACCGTATGACAGATGCTT                                                                        >  SRR4302543.7252049/1‑94 (MQ=60)
                       CTGGCCCAACATGGCCGATGCACGAGCCAGTAGAAACGCCCGAGCAACGTGCATCGATGATTAACTAAACGTGCTTACCGTATGACAGATGCTC                                                                        >  SRR4302543.4115031/1‑94 (MQ=60)
                        TGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTC                                                                       >  SRR4302543.5482745/1‑94 (MQ=60)
                        TGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTC                                                                       >  SRR4302543.7316140/1‑94 (MQ=60)
                         GGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCA                                                                      <  SRR4302543.5408039/94‑1 (MQ=60)
                         GGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCA                                                                      <  SRR4302543.1596539/94‑1 (MQ=60)
                         GGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCA                                                                      <  SRR4302543.1829673/94‑1 (MQ=60)
                          GCCCCACATGTCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAG                                                                     <  SRR4302543.6393693/94‑1 (MQ=60)
                          GCCCCACATGTCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAG                                                                     <  SRR4302543.6393692/94‑1 (MQ=60)
                          GCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAG                                                                     <  SRR4302543.5327707/94‑1 (MQ=60)
                          GCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAG                                                                     <  SRR4302543.1976704/94‑1 (MQ=60)
                           gccgcaatggccgaatgacggcCCAGTAGAAACGGCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGG                                                                    <  SRR4302543.6641099/72‑1 (MQ=60)
                           GCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGG                                                                    <  SRR4302543.6808360/94‑1 (MQ=60)
                           CCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGG                                                                    <  SRR4302543.6641097/94‑1 (MQ=60)
                            CCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGG                                                                   >  SRR4302543.5729866/1‑94 (MQ=60)
                            CCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGG                                                                   >  SRR4302543.2371251/1‑94 (MQ=60)
                            CCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGG                                                                   <  SRR4302543.4255621/94‑1 (MQ=60)
                            CCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGG                                                                   >  SRR4302543.2312533/1‑94 (MQ=60)
                            CCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGG                                                                   >  SRR4302543.3513720/1‑94 (MQ=60)
                            CCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGG                                                                   <  SRR4302543.1995665/94‑1 (MQ=60)
                            CCAACATGGCCGATGCGCGCGCCAGTAGAACCGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGG                                                                   <  SRR4302543.1995664/94‑1 (MQ=60)
                             CCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGC                                                                  <  SRR4302543.5308910/94‑1 (MQ=60)
                             CCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGC                                                                  <  SRR4302543.5154393/94‑1 (MQ=60)
                             CCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGC                                                                  >  SRR4302543.1681677/1‑94 (MQ=60)
                              CACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCA                                                                 >  SRR4302543.7809167/1‑94 (MQ=60)
                              CACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCA                                                                 >  SRR4302543.375956/1‑94 (MQ=60)
                              CACACGGCCGATGCACGCGCCAGTAGAACCGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCA                                                                 <  SRR4302543.4425817/94‑1 (MQ=60)
                               ACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCAC                                                                <  SRR4302543.3695459/94‑1 (MQ=60)
                               ACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCAC                                                                >  SRR4302543.1943176/1‑94 (MQ=60)
                                CATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACT                                                               <  SRR4302543.1372219/94‑1 (MQ=60)
                                CATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACT                                                               <  SRR4302543.1372220/94‑1 (MQ=60)
                                 acgggCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTG                                                              <  SRR4302543.1215495/89‑1 (MQ=60)
                                 ATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTG                                                              >  SRR4302543.7021408/1‑94 (MQ=60)
                                 ATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTG                                                              >  SRR4302543.625264/1‑94 (MQ=60)
                                 ATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTG                                                              >  SRR4302543.4952060/1‑94 (MQ=60)
                                  TGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGG                                                             <  SRR4302543.7763165/94‑1 (MQ=60)
                                  TGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGG                                                             <  SRR4302543.5538477/94‑1 (MQ=60)
                                  TGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGG                                                             <  SRR4302543.3051463/94‑1 (MQ=60)
                                  TGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGG                                                             <  SRR4302543.5329517/94‑1 (MQ=60)
                                   GGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGT                                                            <  SRR4302543.3938026/94‑1 (MQ=60)
                                   GGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGT                                                            <  SRR4302543.3668740/94‑1 (MQ=60)
                                   GGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGT                                                            <  SRR4302543.6806874/94‑1 (MQ=60)
                                   GGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGT                                                            <  SRR4302543.3177936/94‑1 (MQ=60)
                                    GCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTA                                                           <  SRR4302543.6696131/94‑1 (MQ=60)
                                    GCCCATGCCCGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTGCAGGGCACTGGTA                                                           <  SRR4302543.6696128/94‑1 (MQ=60)
                                     CCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAA                                                          <  SRR4302543.6881675/94‑1 (MQ=60)
                                     CCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGAGGCTTCAGGGCACTGGTAA                                                          >  SRR4302543.2635097/1‑94 (MQ=60)
                                      CGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAAC                                                         >  SRR4302543.579447/1‑94 (MQ=60)
                                      CGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAAC                                                         >  SRR4302543.5320200/1‑94 (MQ=60)
                                       GATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACC                                                        >  SRR4302543.1307228/1‑94 (MQ=60)
                                       GATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACC                                                        <  SRR4302543.80874/94‑1 (MQ=60)
                                       GATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACC                                                        <  SRR4302543.945643/94‑1 (MQ=60)
                                        ATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCT                                                       <  SRR4302543.1698536/94‑1 (MQ=60)
                                        ATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCT                                                       <  SRR4302543.3523349/94‑1 (MQ=60)
                                        ATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCT                                                       <  SRR4302543.3339103/94‑1 (MQ=60)
                                         TGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTG                                                      <  SRR4302543.1973492/94‑1 (MQ=60)
                                          GCACGCGCCAGTAGAAACGCCCGAGAACCGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGG                                                     <  SRR4302543.2194708/94‑1 (MQ=60)
                                          GCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGG                                                     <  SRR4302543.7380564/94‑1 (MQ=60)
                                          GCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGG                                                     <  SRR4302543.4519855/94‑1 (MQ=60)
                                           CACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGT                                                    <  SRR4302543.762078/94‑1 (MQ=60)
                                             CGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAG                                                  >  SRR4302543.590916/1‑94 (MQ=60)
                                              GCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAGCGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGG                                                 <  SRR4302543.2941969/94‑1 (MQ=60)
                                              GCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGG                                                 <  SRR4302543.2451972/94‑1 (MQ=60)
                                                GCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTT                                               >  SRR4302543.7361232/1‑94 (MQ=60)
                                                GCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTT                                               >  SRR4302543.6302419/1‑94 (MQ=60)
                                                 CCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTT                                              >  SRR4302543.2312436/1‑94 (MQ=60)
                                                  CAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGCCAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTC                                             >  SRR4302543.970212/1‑94 (MQ=60)
                                                  CAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTT                                             >  SRR4302543.97345/1‑94 (MQ=60)
                                                  CAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTC                                             >  SRR4302543.1052644/1‑94 (MQ=60)
                                                   AGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCT                                            >  SRR4302543.464726/1‑94 (MQ=60)
                                                   AGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCT                                            <  SRR4302543.6450016/94‑1 (MQ=60)
                                                    GTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTT                                           >  SRR4302543.7994328/1‑94 (MQ=60)
                                                    GTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTT                                           >  SRR4302543.7612856/1‑94 (MQ=60)
                                                     TAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTC                                          <  SRR4302543.1708450/94‑1 (MQ=60)
                                                      AGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCC                                         >  SRR4302543.779318/1‑94 (MQ=60)
                                                      AGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCC                                         <  SRR4302543.4168691/94‑1 (MQ=60)
                                                        AAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCAT                                       <  SRR4302543.3641508/94‑1 (MQ=60)
                                                        AAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCAT                                       >  SRR4302543.1341976/1‑94 (MQ=60)
                                                         AACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATC                                      <  SRR4302543.1292834/94‑1 (MQ=60)
                                                          ACGCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCC                                     >  SRR4302543.6231021/1‑94 (MQ=60)
                                                            GCCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCT                                   <  SRR4302543.5106142/94‑1 (MQ=60)
                                                             CCCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTG                                  >  SRR4302543.2330442/1‑94 (MQ=60)
                                                              CCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGT                                 <  SRR4302543.6622154/94‑1 (MQ=60)
                                                              CCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGT                                 >  SRR4302543.3349468/1‑94 (MQ=60)
                                                              CCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGT                                 >  SRR4302543.6243405/1‑94 (MQ=60)
                                                              CCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGT                                 <  SRR4302543.3724121/94‑1 (MQ=60)
                                                              CCGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGT                                 >  SRR4302543.3870166/1‑94 (MQ=60)
                                                               CGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTA                                >  SRR4302543.7484471/1‑94 (MQ=60)
                                                               CGAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTA                                <  SRR4302543.3023761/94‑1 (MQ=60)
                                                                GAGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTAC                               >  SRR4302543.766741/1‑94 (MQ=60)
                                                                 AGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACC                              <  SRR4302543.7920585/94‑1 (MQ=60)
                                                                 AGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACC                              >  SRR4302543.5166114/1‑94 (MQ=60)
                                                                 AGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACC                              >  SRR4302543.7930831/1‑94 (MQ=60)
                                                                 AGAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACC                              >  SRR4302543.4365328/1‑94 (MQ=60)
                                                                  GAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCT                             <  SRR4302543.166082/94‑1 (MQ=60)
                                                                  GAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCT                             <  SRR4302543.5439269/94‑1 (MQ=60)
                                                                  GAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCT                             <  SRR4302543.4155524/94‑1 (MQ=60)
                                                                  GAAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCG                             >  SRR4302543.489183/1‑94 (MQ=60)
                                                                   AAACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTG                            >  SRR4302543.6673553/1‑94 (MQ=60)
                                                                    AACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGA                           >  SRR4302543.3556705/1‑94 (MQ=60)
                                                                    AACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGA                           >  SRR4302543.5673792/1‑94 (MQ=60)
                                                                     ACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAA                          <  SRR4302543.2748629/94‑1 (MQ=60)
                                                                     ACGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAA                          <  SRR4302543.2628130/94‑1 (MQ=60)
                                                                      CGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAG                         <  SRR4302543.7424976/94‑1 (MQ=60)
                                                                      CGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAG                         <  SRR4302543.7430385/94‑1 (MQ=60)
                                                                      CGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAG                         <  SRR4302543.3666240/94‑1 (MQ=60)
                                                                      CGTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAG                         <  SRR4302543.1878770/94‑1 (MQ=60)
                                                                       GTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGG                        <  SRR4302543.3705209/94‑1 (MQ=60)
                                                                       GTGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGG                        >  SRR4302543.2093760/1‑94 (MQ=60)
                                                                        TGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGT                       >  SRR4302543.6261943/1‑94 (MQ=60)
                                                                        TGCATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGGAGGTTTCTTCCATCCCTGTACCTGAAGGT                       >  SRR4302543.7549144/1‑94 (MQ=60)
                                                                          CATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCC                     <  SRR4302543.2705346/94‑1 (MQ=60)
                                                                          CATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCC                     >  SRR4302543.1219743/1‑94 (MQ=60)
                                                                          CATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCC                     >  SRR4302543.6995437/1‑94 (MQ=60)
                                                                          CATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCC                     >  SRR4302543.6282015/1‑94 (MQ=60)
                                                                           ATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCA                    >  SRR4302543.3915014/1‑94 (MQ=60)
                                                                           ATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCA                    >  SRR4302543.2036149/1‑94 (MQ=60)
                                                                           ATCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCA                    <  SRR4302543.1262713/94‑1 (MQ=60)
                                                                            TCGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAA                   <  SRR4302543.405663/94‑1 (MQ=60)
                                                                             CGGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAAC                  <  SRR4302543.3465976/94‑1 (MQ=60)
                                                                              GGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCCTCCCTGTACCTCAAGGTCCAACC                 >  SRR4302543.4738246/1‑94 (MQ=60)
                                                                              GGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACG                 >  SRR4302543.4738250/1‑94 (MQ=60)
                                                                              GGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACG                 >  SRR4302543.2246084/1‑94 (MQ=60)
                                                                              GGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACG                 <  SRR4302543.3704530/94‑1 (MQ=60)
                                                                              GGTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACC                 >  SRR4302543.916122/1‑94 (MQ=60)
                                                                               GTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGC                <  SRR4302543.1712913/94‑1 (MQ=60)
                                                                               GTGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGC                >  SRR4302543.6896439/1‑94 (MQ=60)
                                                                                TGATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCC               >  SRR4302543.7331151/1‑94 (MQ=60)
                                                                                 GATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCA              >  SRR4302543.3221739/1‑94 (MQ=60)
                                                                                  ATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCACCGCCAA             >  SRR4302543.5493884/1‑94 (MQ=60)
                                                                                  ATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAA             >  SRR4302543.6968847/1‑94 (MQ=60)
                                                                                  ATTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAA             >  SRR4302543.5493883/1‑94 (MQ=60)
                                                                                   TTAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAAT            <  SRR4302543.5808324/94‑1 (MQ=60)
                                                                                    TAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATA           <  SRR4302543.5461723/94‑1 (MQ=60)
                                                                                    TAACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATA           <  SRR4302543.1964662/94‑1 (MQ=60)
                                                                                     AACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAA          <  SRR4302543.5171478/94‑1 (MQ=60)
                                                                                      ACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAAT         <  SRR4302543.3477038/94‑1 (MQ=60)
                                                                                      ACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAAT         <  SRR4302543.2718403/94‑1 (MQ=60)
                                                                                      ACGAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAAT         <  SRR4302543.2718402/94‑1 (MQ=60)
                                                                                        GAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGA       >  SRR4302543.4105122/1‑94 (MQ=60)
                                                                                        GAAACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGA       <  SRR4302543.2509569/94‑1 (MQ=60)
                                                                                          AACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACC     >  SRR4302543.6177/1‑94 (MQ=60)
                                                                                          AACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACC     <  SRR4302543.6941036/94‑1 (MQ=60)
                                                                                           ACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCA    <  SRR4302543.5771288/94‑1 (MQ=60)
                                                                                           ACGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCA    >  SRR4302543.7280435/1‑94 (MQ=60)
                                                                                            CGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAG   <  SRR4302543.6969613/94‑1 (MQ=60)
                                                                                            CGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAG   <  SRR4302543.227474/94‑1 (MQ=60)
                                                                                            CGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAG   <  SRR4302543.7503587/94‑1 (MQ=60)
                                                                                            CGTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAG   >  SRR4302543.6498396/1‑94 (MQ=60)
                                                                                             GTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGA  >  SRR4302543.6009316/1‑94 (MQ=60)
                                                                                             GTGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGA  <  SRR4302543.4863469/94‑1 (MQ=60)
                                                                                             |                                                                                             
GATGGGGCCTCCGGCCAGCGCTTCTGGCCCCACATGGCCGATGCACGCGCCAGTAGAAACGCCCGAGAAACGTGCATCGGTGATTAACGAAACATGCTTACCGTATGACAGATGCTTCAGGGCACTGGTAACCTGGTAGGTTTCTTCCATCCCTGTACCTGAAGGTCCAACGCCAATAATGACCAGA  >  NC_000913/4519989‑4520175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑