Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 111,893 A→G V236A (GTA→GCA)  yacF ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913111,8930AG96.8% 555.2 / 4.1 188V236A (GTA→GCA) yacFhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (2/4);  new base G (91/91);  total (93/95)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 192 total aligned reads displayed.

GTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGC  >  NC_000913/111803‑111982
                                                                                          |                                                                                         
gTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGcc                                                                                         <  2:1495925/93‑1 (MQ=255)
gTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGcc                                                                                         >  1:2854837/1‑93 (MQ=255)
  tttCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTg                                                                                       <  1:1143495/93‑1 (MQ=255)
     cccGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTcc                                                                                    <  2:575075/93‑1 (MQ=255)
     cccGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTcc                                                                                    <  2:3423385/93‑1 (MQ=255)
          aGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGtttt                                                                               >  2:3605993/1‑93 (MQ=255)
           ggTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTc                                                                              <  2:4055829/93‑1 (MQ=255)
            gTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCa                                                                             >  1:130663/1‑93 (MQ=255)
            gTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCa                                                                             >  1:2190948/1‑93 (MQ=255)
             ttGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAg                                                                            <  2:3848217/93‑1 (MQ=255)
             ttGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAg                                                                            <  1:1739371/93‑1 (MQ=255)
              tGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAgt                                                                           <  2:4511048/93‑1 (MQ=255)
              tGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAgt                                                                           <  1:4628930/93‑1 (MQ=255)
                 gCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGATCCGGTGCCTGTCCGTTTTCAGTGTc                                                                        >  1:4162437/1‑93 (MQ=255)
                  cAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTcc                                                                       <  1:4390687/93‑1 (MQ=255)
                   aaTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCa                                                                      <  2:157617/93‑1 (MQ=255)
                   aaTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCa                                                                      >  2:2806438/1‑93 (MQ=255)
                    aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAg                                                                     >  2:3547931/1‑93 (MQ=255)
                    aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAg                                                                     >  2:1612675/1‑93 (MQ=255)
                    aTTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAg                                                                     >  1:975872/1‑93 (MQ=255)
                     ttCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGc                                                                    <  2:3303353/93‑1 (MQ=255)
                     ttCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGc                                                                    >  1:2434170/1‑93 (MQ=255)
                      tCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCg                                                                   <  1:2230226/93‑1 (MQ=255)
                         ccGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCa                                                                >  2:4473823/1‑93 (MQ=255)
                          cGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCAt                                                               >  1:3559857/1‑93 (MQ=255)
                          cGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCAt                                                               >  1:1063841/1‑93 (MQ=255)
                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  2:1375279/1‑93 (MQ=255)
                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  1:3801808/1‑93 (MQ=255)
                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  2:2718671/1‑93 (MQ=255)
                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  1:4041570/1‑93 (MQ=255)
                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTATTCAGTGTCCAGCGGCATa                                                              >  2:3183031/1‑93 (MQ=255)
                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGGTTTCAGTGTCCAGCGGCATa                                                              >  2:3183032/1‑93 (MQ=255)
                           gTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  1:1567188/1‑93 (MQ=255)
                           gTAATAGTTTCTGACATTTCTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATa                                                              >  2:4565769/1‑93 (MQ=255)
                            tAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaa                                                             >  2:2027344/1‑93 (MQ=255)
                            tAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaa                                                             >  2:3017062/1‑93 (MQ=255)
                            tAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaa                                                             >  1:3467265/1‑93 (MQ=255)
                              aTAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaaaa                                                           <  2:4126013/93‑1 (MQ=255)
                              aTAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCAAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaaaa                                                           <  1:3346229/93‑1 (MQ=255)
                              aTAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTGCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATaaaa                                                           <  2:1510516/93‑1 (MQ=255)
                                 gTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGa                                                        <  2:233566/93‑1 (MQ=255)
                                  tttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGaa                                                       >  2:1471210/1‑93 (MQ=255)
                                  tttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGaa                                                       >  2:3965989/1‑93 (MQ=255)
                                  tttCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGaa                                                       >  2:279369/1‑93 (MQ=255)
                                     cTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATgg                                                    <  1:4141082/93‑1 (MQ=255)
                                      tGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGc                                                   >  1:2025090/1‑93 (MQ=255)
                                       gACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCa                                                  >  1:1889770/1‑93 (MQ=255)
                                       gACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCa                                                  <  2:4672653/93‑1 (MQ=255)
                                       gACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCa                                                  <  2:718903/93‑1 (MQ=255)
                                       gACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCa                                                  >  1:941614/1‑93 (MQ=255)
                                            ttttACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACg                                             <  1:3365186/93‑1 (MQ=255)
                                              ttACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGc                                           <  1:4011374/93‑1 (MQ=255)
                                                aCTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctc                                         <  2:815104/93‑1 (MQ=255)
                                                aCTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctc                                         >  1:3275788/1‑93 (MQ=255)
                                                aCTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctc                                         <  1:3611016/93‑1 (MQ=255)
                                                aCTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctc                                         <  1:3309814/93‑1 (MQ=255)
                                                 cTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGctct                                        <  1:1512893/93‑1 (MQ=255)
                                                   ccTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCttc                                      >  2:1048655/1‑92 (MQ=255)
                                                   ccTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTa                                      <  2:2394499/93‑1 (MQ=255)
                                                   ccTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTa                                      <  1:2614649/93‑1 (MQ=255)
                                                    cTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTAt                                     <  2:4216132/93‑1 (MQ=255)
                                                    cTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTAt                                     <  1:3334741/93‑1 (MQ=255)
                                                     ttAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATg                                    >  1:2882017/1‑93 (MQ=255)
                                                     ttAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATg                                    >  1:3912204/1‑93 (MQ=255)
                                                     ttAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATg                                    >  1:1264947/1‑93 (MQ=255)
                                                      tAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGt                                   <  1:2967400/93‑1 (MQ=255)
                                                       aGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTc                                  <  1:901531/93‑1 (MQ=255)
                                                        gCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTcc                                 <  1:2731729/93‑1 (MQ=255)
                                                        gCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTcc                                 >  2:173277/1‑93 (MQ=255)
                                                        gCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTcc                                 >  2:4280703/1‑93 (MQ=255)
                                                         cAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCg                                <  2:2295084/93‑1 (MQ=255)
                                                          aaCAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCgg                               >  2:1919223/1‑93 (MQ=255)
                                                              ggCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAt                           <  1:638225/93‑1 (MQ=255)
                                                               gCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAtt                          <  1:2345382/93‑1 (MQ=255)
                                                               gCCAGTTCGAAAACCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAtt                          <  2:916139/93‑1 (MQ=255)
                                                                ccAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAttt                         <  2:873020/93‑1 (MQ=255)
                                                                ccAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAttt                         >  1:428925/1‑93 (MQ=255)
                                                                  aGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGc                       <  2:2457269/93‑1 (MQ=255)
                                                                  aGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGc                       >  2:4145603/1‑93 (MQ=255)
                                                                   gTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCg                      >  2:261247/1‑93 (MQ=255)
                                                                   gTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCg                      <  2:634293/93‑1 (MQ=255)
                                                                    ttCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCgg                     <  2:474972/93‑1 (MQ=255)
                                                                    ttCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCgg                     <  1:4122470/93‑1 (MQ=255)
                                                                      cGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGAt                   <  1:1108379/93‑1 (MQ=255)
                                                                       gAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATa                  >  1:2746942/1‑93 (MQ=255)
                                                                         aaTCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATaaa                <  2:2143020/93‑1 (MQ=255)
                                                                          aTCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAg               >  1:706003/1‑93 (MQ=255)
                                                                          aTCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAg               <  1:545143/93‑1 (MQ=255)
                                                                          aTCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAg               >  1:3157174/1‑93 (MQ=255)
                                                                           tCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGc              <  1:1352625/93‑1 (MQ=255)
                                                                           tCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGc              <  2:3260442/93‑1 (MQ=255)
                                                                           tCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAGTTGCGGATAAAGc              >  1:3770595/1‑93 (MQ=255)
                                                                           tCCAGACGTTCCGGTGCCTGGCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGc              <  1:3816855/93‑1 (MQ=255)
                                                                                 cGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGaa        <  1:845559/93‑1 (MQ=255)
                                                                                  gTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAAt       >  2:1975760/1‑93 (MQ=255)
                                                                                   ttCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATc      >  1:4557422/1‑93 (MQ=255)
                                                                                   ttCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATc      >  2:180859/1‑93 (MQ=255)
                                                                                    tCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCg     >  2:1326487/1‑93 (MQ=255)
                                                                                     ccGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGa    <  2:1852611/93‑1 (MQ=255)
                                                                                       ggTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGc  >  1:880423/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGC  >  NC_000913/111803‑111982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGAC  >  NC_000913/111802‑111985
                                                                                           |                                                                                            
CGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCC                                                                                            >  SRR4302535.2875061/1‑94 (MQ=60)
CGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCC                                                                                            >  SRR4302535.3353951/1‑94 (MQ=60)
   TTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGT                                                                                         <  SRR4302535.1151648/94‑1 (MQ=60)
      CCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCG                                                                                      >  SRR4302535.2815190/1‑94 (MQ=60)
      CCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCG                                                                                      <  SRR4302535.2226456/94‑1 (MQ=60)
       CCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGT                                                                                     <  SRR4302535.319856/94‑1 (MQ=60)
           AGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTC                                                                                 <  SRR4302535.1446449/94‑1 (MQ=60)
            GGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCA                                                                                >  SRR4302535.2206544/1‑94 (MQ=60)
            GGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCA                                                                                >  SRR4302535.131580/1‑94 (MQ=60)
             GTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAG                                                                               >  SRR4302535.3834836/1‑94 (MQ=60)
              TTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGT                                                                              <  SRR4302535.1751940/94‑1 (MQ=60)
               TGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTG                                                                             <  SRR4302535.181649/94‑1 (MQ=60)
               TGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTG                                                                             <  SRR4302535.4660345/94‑1 (MQ=60)
                GGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGT                                                                            >  SRR4302535.1559578/1‑94 (MQ=60)
                 GGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGATCCGGTGCCTGTCCGTTTTCAGTGTC                                                                           >  SRR4302535.4190926/1‑94 (MQ=60)
                   CAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCA                                                                         <  SRR4302535.4420730/94‑1 (MQ=60)
                    AATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAG                                                                        >  SRR4302535.982799/1‑94 (MQ=60)
                     ATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGC                                                                       >  SRR4302535.2451705/1‑94 (MQ=60)
                      TTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCG                                                                      <  SRR4302535.2413642/94‑1 (MQ=60)
                       TCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGG                                                                     <  SRR4302535.2246085/94‑1 (MQ=60)
                        TACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGC                                                                    <  SRR4302535.3805791/94‑1 (MQ=60)
                        CACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTNTCCGTTTTCAGTGNCNANCGGC                                                                    >  SRR4302535.3564210/1‑94 (MQ=60)
                         ACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCA                                                                   >  SRR4302535.368969/1‑94 (MQ=60)
                          CCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCAT                                                                  >  SRR4302535.3584542/1‑94 (MQ=60)
                          CCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCAT                                                                  <  SRR4302535.2086144/94‑1 (MQ=60)
                          CCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCAT                                                                  >  SRR4302535.1071412/1‑94 (MQ=60)
                           CGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATA                                                                 >  SRR4302535.4069240/1‑94 (MQ=60)
                           CGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATA                                                                 >  SRR4302535.3828014/1‑94 (MQ=60)
                           CGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATA                                                                 <  SRR4302535.3331138/94‑1 (MQ=60)
                           CGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATA                                                                 >  SRR4302535.1578497/1‑94 (MQ=60)
                            GTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAA                                                                >  SRR4302535.3491359/1‑94 (MQ=60)
                             TAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAA                                                               >  SRR4302535.4538900/1‑94 (MQ=60)
                             TAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAA                                                               <  SRR4302535.951840/94‑1 (MQ=60)
                              AATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAA                                                              >  SRR4302535.3569664/1‑94 (MQ=60)
                               ATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAAC                                                             <  SRR4302535.163302/94‑1 (MQ=60)
                               ATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAAC                                                             >  SRR4302535.4164608/1‑94 (MQ=60)
                               ATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCAAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAAC                                                             <  SRR4302535.3369540/94‑1 (MQ=60)
                                TAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACG                                                            <  SRR4302535.1951724/94‑1 (MQ=60)
                                  GTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAA                                                          >  SRR4302535.465916/1‑94 (MQ=60)
                                    ttcgcACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATG                                                        <  SRR4302535.3853955/89‑1 (MQ=60)
                                    TTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATG                                                        >  SRR4302535.70303/1‑94 (MQ=60)
                                    TTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATG                                                        <  SRR4302535.3930746/94‑1 (MQ=60)
                                     TCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGG                                                       >  SRR4302535.1293135/1‑94 (MQ=60)
                                      CTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGC                                                      >  SRR4302535.2039683/1‑94 (MQ=60)
                                      CTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGC                                                      <  SRR4302535.4169437/94‑1 (MQ=60)
                                       TGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCA                                                     >  SRR4302535.948279/1‑94 (MQ=60)
                                       TGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCA                                                     >  SRR4302535.1903381/1‑94 (MQ=60)
                                             TTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGG                                               <  SRR4302535.2751259/94‑1 (MQ=60)
                                             TTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGG                                               <  SRR4302535.823551/94‑1 (MQ=60)
                                             TTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGG                                               <  SRR4302535.3388603/94‑1 (MQ=60)
                                             TTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGG                                               >  SRR4302535.901320/1‑94 (MQ=60)
                                               TTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCT                                             <  SRR4302535.4038817/94‑1 (MQ=60)
                                                TACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTC                                            >  SRR4302535.3298542/1‑94 (MQ=60)
                                                 ACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCT                                           <  SRR4302535.1221605/94‑1 (MQ=60)
                                                 ACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCT                                           <  SRR4302535.3636057/94‑1 (MQ=60)
                                                 ACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCT                                           <  SRR4302535.3332844/94‑1 (MQ=60)
                                                  CTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTT                                          <  SRR4302535.1523792/94‑1 (MQ=60)
                                                    CCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTAT                                        >  SRR4302535.3484029/1‑94 (MQ=60)
                                                    CCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTAT                                        <  SRR4302535.2633399/94‑1 (MQ=60)
                                                    CCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTAT                                        <  SRR4302535.4214266/94‑1 (MQ=60)
                                                     CTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATG                                       <  SRR4302535.2985957/94‑1 (MQ=60)
                                                     CTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATG                                       >  SRR4302535.2902414/1‑94 (MQ=60)
                                                     CTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATG                                       >  SRR4302535.539309/1‑94 (MQ=60)
                                                     CTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATG                                       >  SRR4302535.1273995/1‑94 (MQ=60)
                                                     CTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATG                                       >  SRR4302535.3938985/1‑94 (MQ=60)
                                                     CTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATG                                       <  SRR4302535.3357967/94‑1 (MQ=60)
                                                      TTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGT                                      <  SRR4302535.3157662/94‑1 (MQ=60)
                                                       TAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTC                                     <  SRR4302535.2988222/94‑1 (MQ=60)
                                                        AGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCC                                    <  SRR4302535.907914/94‑1 (MQ=60)
                                                         GCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCG                                   <  SRR4302535.2751231/94‑1 (MQ=60)
                                                           AACAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGA                                 >  SRR4302535.3361092/1‑94 (MQ=60)
                                                             CAGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAA                               <  SRR4302535.873056/94‑1 (MQ=60)
                                                              AGGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCGTGAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAT                              >  SRR4302535.2517496/1‑94 (MQ=60)
                                                               GGCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATT                             <  SRR4302535.642686/94‑1 (MQ=60)
                                                                GCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTT                            >  SRR4302535.431907/1‑94 (MQ=60)
                                                                GCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTT                            >  SRR4302535.2875310/1‑94 (MQ=60)
                                                                GCCAGTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTT                            <  SRR4302535.2362189/94‑1 (MQ=60)
                                                                    GTTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGG                        <  SRR4302535.4040489/94‑1 (MQ=60)
                                                                     TTCGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGA                       <  SRR4302535.4150680/94‑1 (MQ=60)
                                                                       CGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATA                     >  SRR4302535.2766523/1‑94 (MQ=60)
                                                                       CGAAATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATA                     <  SRR4302535.1116297/94‑1 (MQ=60)
                                                                          AATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAG                  >  SRR4302535.710962/1‑94 (MQ=60)
                                                                          AATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAG                  >  SRR4302535.3179093/1‑94 (MQ=60)
                                                                          AATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAG                  <  SRR4302535.4365124/94‑1 (MQ=60)
                                                                           ATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGC                 <  SRR4302535.548952/94‑1 (MQ=60)
                                                                           ATCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAAGTTGCGGATAAAGC                 >  SRR4302535.3796614/1‑94 (MQ=60)
                                                                            TCCAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCT                <  SRR4302535.1362305/94‑1 (MQ=60)
                                                                            TCCAGACGTTCCGGTGCCTGGCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCT                <  SRR4302535.3843192/94‑1 (MQ=60)
                                                                              CAGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGT              <  SRR4302535.629653/94‑1 (MQ=60)
                                                                               AGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTG             >  SRR4302535.3593449/1‑94 (MQ=60)
                                                                               AGACGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTG             <  SRR4302535.4006507/94‑1 (MQ=60)
                                                                                  CGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAAT          >  SRR4302535.4107539/1‑94 (MQ=60)
                                                                                  CGTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAAT          <  SRR4302535.851494/94‑1 (MQ=60)
                                                                                   GTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATC         >  SRR4302535.4588542/1‑94 (MQ=60)
                                                                                   GTTCCGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATC         <  SRR4302535.1340096/94‑1 (MQ=60)
                                                                                       CGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGC     >  SRR4302535.886656/1‑94 (MQ=60)
                                                                                       CGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGC     >  SRR4302535.937638/1‑94 (MQ=60)
                                                                                       CGGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGC     >  SRR4302535.11239/1‑94 (MQ=60)
                                                                                        GGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCG    >  SRR4302535.247778/1‑94 (MQ=60)
                                                                                        GGTGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCG    <  SRR4302535.2717746/94‑1 (MQ=60)
                                                                                          TGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGAC  <  SRR4302535.1205661/94‑1 (MQ=60)
                                                                                          TGCCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGAC  >  SRR4302535.113513/1‑94 (MQ=60)
                                                                                           |                                                                                            
CGTTTTCCCGCAGGTTGGGCAATTCACCGTAATAGTTTCTGACATTTTTACTCCTTAGCAACAGGCCAGTTCGAAATCCAGACGTTCCGGTACCTGTCCGTTTTCAGTGTCCAGCGGCATAAAACGAATGGCAAAACGGCTCTTATGTCCGGAAATTTGCGGATAAAGCTGTGAATCGAGCGAC  >  NC_000913/111802‑111985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑