Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,953,902 T→C Q377R (CAG→CGG)  b1872 ← biotin sulfoxide reductase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,953,9020TC99.1% 336.4 / ‑4.9 108Q377R (CAG→CGG) b1872biotin sulfoxide reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (50/57);  total (50/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 113 total aligned reads displayed.

ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953812‑1953991
                                                                                          |                                                                                         
aTTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGc                                                                                         >  1:3177230/1‑93 (MQ=255)
 tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGcc                                                                                        <  2:725314/93‑1 (MQ=255)
  ttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGccc                                                                                       <  1:684802/93‑1 (MQ=255)
    ccGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTa                                                                                     >  2:1902072/1‑93 (MQ=255)
       gcagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACa                                                                                  >  2:4425630/1‑93 (MQ=255)
       gcagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACa                                                                                  <  1:2418792/93‑1 (MQ=255)
       gcagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACa                                                                                  <  2:2615194/93‑1 (MQ=255)
           caCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgc                                                                              >  2:2883794/1‑93 (MQ=255)
             cgccgcCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgccg                                                                            >  1:1435431/1‑93 (MQ=255)
             cgccgcCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgccg                                                                            >  2:1528787/1‑93 (MQ=255)
              gccgccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgccgc                                                                           <  1:255402/93‑1 (MQ=255)
              cccgccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgccgc                                                                           <  1:1000813/92‑1 (MQ=255)
               ccgccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCgccgcc                                                                          >  2:4439488/1‑93 (MQ=255)
               ccgccAACACGCGTCGGGTGGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCgccgcc                                                                          <  2:1639972/93‑1 (MQ=255)
                cgcgAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCa                                                                         <  1:1744592/93‑1 (MQ=255)
                 gccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAg                                                                        <  1:336275/93‑1 (MQ=255)
                    aaCACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGt                                                                     >  1:2945865/1‑93 (MQ=255)
                    aaCACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGt                                                                     <  1:2705572/93‑1 (MQ=255)
                     acacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTc                                                                    <  1:1917320/93‑1 (MQ=255)
                     acacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTc                                                                    >  1:1076858/1‑93 (MQ=255)
                     acacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTc                                                                    >  1:4159065/1‑93 (MQ=255)
                     acacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCACCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTc                                                                    <  1:3296321/93‑1 (MQ=255)
                      cacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCa                                                                   <  2:180350/93‑1 (MQ=255)
                      cacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCa                                                                   >  2:4009958/1‑93 (MQ=255)
                      cacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCa                                                                   <  1:1558045/93‑1 (MQ=255)
                       acGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCAc                                                                  <  1:1691019/93‑1 (MQ=255)
                          cgTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTCCCAATCCGCCCTAACATCGCCGCCAGCGTCACCAg                                                               >  1:3549745/1‑93 (MQ=255)
                           gTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGc                                                              <  2:3446781/93‑1 (MQ=255)
                           gTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGc                                                              <  1:4237082/93‑1 (MQ=255)
                            tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  2:37541/1‑93 (MQ=255)
                             cGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCAt                                                            <  1:714207/93‑1 (MQ=255)
                                gTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCa                                                         <  1:3814998/93‑1 (MQ=255)
                                gTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCa                                                         <  1:481013/93‑1 (MQ=255)
                                gTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCa                                                         <  1:2868284/93‑1 (MQ=255)
                                gTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCa                                                         >  2:3893162/1‑93 (MQ=255)
                                 ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                                        <  1:2701620/93‑1 (MQ=255)
                                  tGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgt                                                       <  2:1906940/93‑1 (MQ=255)
                                  tGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgt                                                       <  1:3912499/93‑1 (MQ=255)
                                  tGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgt                                                       <  2:807733/93‑1 (MQ=255)
                                     cACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGtt                                                    <  2:1754515/93‑1 (MQ=255)
                                       ccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                                                  <  1:4227354/93‑1 (MQ=255)
                                        cGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCt                                                 >  2:3984187/1‑93 (MQ=255)
                                        cGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCt                                                 <  2:2289154/93‑1 (MQ=255)
                                         gTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                                                <  2:1741453/93‑1 (MQ=255)
                                         gTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                                                >  2:1018767/1‑93 (MQ=255)
                                          ttGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGt                                               >  2:4006557/1‑93 (MQ=255)
                                              aGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAc                                           <  1:3529909/93‑1 (MQ=255)
                                                tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         >  2:2173661/1‑93 (MQ=255)
                                                tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         >  1:4591535/1‑93 (MQ=255)
                                                 aaTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCat                                        <  2:256434/93‑1 (MQ=255)
                                                  atcaTAAGAGAAACCAAAACCGCCGCCCGGTCTCCCAACCCGCCCTAACACCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCata                                       <  2:1399646/90‑1 (MQ=37)
                                                  aTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCata                                       <  2:1780605/93‑1 (MQ=255)
                                                    gATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     <  2:1790211/93‑1 (MQ=255)
                                                    gATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     <  2:635460/93‑1 (MQ=255)
                                                    gATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     >  1:1840274/1‑93 (MQ=255)
                                                    gATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     >  1:46518/1‑93 (MQ=255)
                                                      tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc                                   >  2:1000368/1‑93 (MQ=255)
                                                      tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc                                   <  2:3407635/93‑1 (MQ=255)
                                                      tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc                                   <  2:1518299/93‑1 (MQ=255)
                                                       aaGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt                                  <  1:2819874/93‑1 (MQ=255)
                                                       aaGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt                                  <  1:3132127/93‑1 (MQ=255)
                                                        agagAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTg                                 >  2:2269770/1‑93 (MQ=255)
                                                        agagAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTg                                 <  1:2166495/93‑1 (MQ=255)
                                                        agagAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTg                                 <  2:226859/93‑1 (MQ=255)
                                                        agagAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTg                                 <  2:2945865/93‑1 (MQ=255)
                                                         gagaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTgg                                >  1:2051074/1‑93 (MQ=255)
                                                         gagaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTgg                                <  1:3105288/93‑1 (MQ=255)
                                                           gaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgcg                              >  2:1283147/1‑93 (MQ=255)
                                                           gaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgcg                              >  2:2498363/1‑93 (MQ=255)
                                                           gaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgcg                              >  1:113176/1‑93 (MQ=255)
                                                            aaaCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgcgc                             <  2:4337425/93‑1 (MQ=255)
                                                             aaCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCt                            >  1:3762891/1‑93 (MQ=255)
                                                             aaCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCt                            >  1:4103831/1‑93 (MQ=255)
                                                              aCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTg                           >  1:998937/1‑93 (MQ=255)
                                                                cAAAACCGCCGCCCGGTGTCCCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa                         >  2:4578606/1‑93 (MQ=255)
                                                                cAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa                         <  2:1378847/93‑1 (MQ=255)
                                                                   aaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc                      >  1:2081052/1‑93 (MQ=255)
                                                                     ccgccgCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc                    >  1:2078530/1‑93 (MQ=255)
                                                                     ccgccgCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc                    >  1:3618839/1‑93 (MQ=255)
                                                                      cgtcgccCGGTGTACCAACCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc                   <  1:751722/90‑1 (MQ=255)
                                                                       gccgccCGGTGTACCAATCCGCCCTAGCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa                  <  2:806294/93‑1 (MQ=255)
                                                                         cgccCGGTGTACCACTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGc                <  1:2561197/93‑1 (MQ=255)
                                                                          gccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGcc               >  2:2885755/1‑93 (MQ=255)
                                                                          gccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGcc               >  1:2664736/1‑93 (MQ=255)
                                                                          gccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGcc               <  2:4507715/93‑1 (MQ=255)
                                                                             cGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc            >  2:13073/1‑93 (MQ=255)
                                                                              ggTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGcc           >  1:658777/1‑93 (MQ=255)
                                                                               gtgtCCCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa          <  2:226626/93‑1 (MQ=255)
                                                                               gtgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa          <  1:4435698/93‑1 (MQ=255)
                                                                               gtgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa          >  2:4066911/1‑93 (MQ=255)
                                                                                  tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  1:822952/93‑1 (MQ=255)
                                                                                  tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  1:2745034/93‑1 (MQ=255)
                                                                                  tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       >  1:2355235/1‑93 (MQ=255)
                                                                                    ccAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGc     <  1:1048996/93‑1 (MQ=255)
                                                                                     cAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa    >  1:3965583/1‑93 (MQ=255)
                                                                                      aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGcct   >  1:1341723/1‑91 (MQ=255)
                                                                                      aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt   >  1:4392868/1‑93 (MQ=255)
                                                                                      aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt   <  1:3585870/93‑1 (MQ=255)
                                                                                       aTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAtt  >  1:3244957/1‑93 (MQ=255)
                                                                                       aTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAtt  <  1:1221608/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953812‑1953991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  >  NC_000913/1953809‑1953995
                                                                                             |                                                                                             
GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCC                                                                                               >  SRR4302535.2530562/1‑94 (MQ=60)
  CATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGC                                                                                             >  SRR4302535.3199208/1‑94 (MQ=60)
     TTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCT                                                                                          <  SRR4302535.689599/94‑1 (MQ=60)
       CCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAA                                                                                        <  SRR4302535.4578111/94‑1 (MQ=60)
        CGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACAAAAACCGCCGCCCGGTGAACCAATCCCCCCTAAC                                                                                       >  SRR4302535.3897674/1‑94 (MQ=60)
          GCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAT                                                                                     <  SRR4302535.2436206/94‑1 (MQ=60)
            AGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCC                                                                                   >  SRR4302535.2492614/1‑94 (MQ=60)
               ACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCG                                                                                >  SRR4302535.1445686/1‑94 (MQ=60)
                 GCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCC                                                                              <  SRR4302535.257162/94‑1 (MQ=60)
                 CCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCC                                                                              <  SRR4302535.1007926/94‑1 (MQ=60)
                   CGCGAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAG                                                                            <  SRR4302535.1757216/94‑1 (MQ=60)
                    GCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGC                                                                           <  SRR4302535.338601/94‑1 (MQ=60)
                      CAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGT                                                                         >  SRR4302535.2966567/1‑94 (MQ=60)
                       AACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTC                                                                        >  SRR4302535.4187530/1‑94 (MQ=60)
                       AACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTC                                                                        <  SRR4302535.2724907/94‑1 (MQ=60)
                       AACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTC                                                                        >  SRR4302535.1084516/1‑94 (MQ=60)
                        ACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCA                                                                       <  SRR4302535.1931136/94‑1 (MQ=60)
                        ACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCACCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCA                                                                       <  SRR4302535.3319239/94‑1 (MQ=60)
                         CACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCAC                                                                      <  SRR4302535.1569277/94‑1 (MQ=60)
                          ACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACC                                                                     <  SRR4302535.1703256/94‑1 (MQ=60)
                            GCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTCCCAATCCGCCCTAACATCGCCGCCAGCGTCACCAG                                                                   >  SRR4302535.3574382/1‑94 (MQ=60)
                              GTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCA                                                                 <  SRR4302535.4266078/94‑1 (MQ=60)
                                CGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATC                                                               <  SRR4302535.719223/94‑1 (MQ=60)
                                   GTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAG                                                            <  SRR4302535.3841321/94‑1 (MQ=60)
                                   GTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAG                                                            <  SRR4302535.2888599/94‑1 (MQ=60)
                                   GTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAG                                                            <  SRR4302535.484386/94‑1 (MQ=60)
                                    TTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGT                                                           <  SRR4302535.2720921/94‑1 (MQ=60)
                                     TGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTG                                                          <  SRR4302535.3939281/94‑1 (MQ=60)
                                      GCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGT                                                         >  SRR4302535.2794124/1‑94 (MQ=60)
                                        CACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTT                                                       >  SRR4302535.2330577/1‑94 (MQ=60)
                                          CCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCT                                                     <  SRR4302535.4256282/94‑1 (MQ=60)
                                                 AGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACC                                              <  SRR4302535.3554418/94‑1 (MQ=60)
                                                  GTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCA                                             >  SRR4302535.4622790/1‑94 (MQ=60)
                                                      TGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATT                                         >  SRR4302535.1853548/1‑94 (MQ=60)
                                                      TGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATT                                         >  SRR4302535.46870/1‑94 (MQ=60)
                                                          AAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTG                                     <  SRR4302535.2839902/94‑1 (MQ=60)
                                                          AAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTG                                     <  SRR4302535.3153940/94‑1 (MQ=60)
                                                           AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                    >  SRR4302535.2065844/1‑94 (MQ=60)
                                                           AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                    <  SRR4302535.2182024/94‑1 (MQ=60)
                                                            GAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGC                                   <  SRR4302535.3126906/94‑1 (MQ=60)
                                                             AGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCG                                  >  SRR4302535.113968/1‑94 (MQ=60)
                                                               AAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCT                                >  SRR4302535.3788887/1‑94 (MQ=60)
                                                               AAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCT                                >  SRR4302535.4131926/1‑94 (MQ=60)
                                                                AACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTG                               >  SRR4302535.1006022/1‑94 (MQ=60)
                                                                   CAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAT                            <  SRR4302535.3306422/94‑1 (MQ=60)
                                                                     AAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC                          >  SRR4302535.2096045/1‑94 (MQ=60)
                                                                      ATCCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCC                         <  SRR4302535.1662090/94‑1 (MQ=60)
                                                                       ACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCC                        >  SRR4302535.3643926/1‑94 (MQ=60)
                                                                       ACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCC                        >  SRR4302535.2093503/1‑94 (MQ=60)
                                                                         CGTCGCCCGGTGTACCAACCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCA                      <  SRR4302535.757014/94‑1 (MQ=60)
                                                                            CGCCCGGTGTACCACTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCC                   <  SRR4302535.2579636/94‑1 (MQ=60)
                                                                            CGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCC                   >  SRR4302535.2683813/1‑94 (MQ=60)
                                                                                CGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCC               >  SRR4302535.663375/1‑94 (MQ=60)
                                                                                  GTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAT             <  SRR4302535.4466098/94‑1 (MQ=60)
                                                                                    GTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCA           >  SRR4302535.2372125/1‑94 (MQ=60)
                                                                                     TACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAG          <  SRR4302535.828721/94‑1 (MQ=60)
                                                                                     TACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAG          <  SRR4302535.2764600/94‑1 (MQ=60)
                                                                                       CCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCA        >  SRR4302535.3992672/1‑94 (MQ=60)
                                                                                       CCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCA        <  SRR4302535.1056457/94‑1 (MQ=60)
                                                                                        CAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCCT       >  SRR4302535.1351296/1‑94 (MQ=60)
                                                                                        CAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAT       >  SRR4302535.4422925/1‑94 (MQ=60)
                                                                                         AATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT      <  SRR4302535.3610744/94‑1 (MQ=60)
                                                                                         AATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT      >  SRR4302535.784962/1‑94 (MQ=60)
                                                                                         AATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT      >  SRR4302535.3267494/1‑94 (MQ=60)
                                                                                          ATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG     <  SRR4302535.1230360/94‑1 (MQ=60)
                                                                                           TCCGCCCTAGCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGT    <  SRR4302535.107966/94‑1 (MQ=60)
                                                                                            CCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTG   >  SRR4302535.3921330/1‑94 (MQ=60)
                                                                                            CCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTG   >  SRR4302535.622709/1‑94 (MQ=60)
                                                                                             CGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  >  SRR4302535.3846573/1‑94 (MQ=60)
                                                                                             |                                                                                             
GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  >  NC_000913/1953809‑1953995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑