Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,953,902	T→C	Q377R (CAG→CGG)	b1872 ←	biotin sulfoxide reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,953,902	0	T	C	99.5%	610.7 / ‑4.7	195	Q377R (CAG→CGG)	b1872	biotin sulfoxide reductase 2
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (110/84); total (111/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

Only 100 of 205 total aligned reads displayed.

TTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953813‑1953991

tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGcc                                                                                        >  1:657933/1‑93 (MQ=255)

tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGcc                                                                                        >  2:100134/1‑93 (MQ=255)

tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGcc                                                                                        >  1:3118854/1‑93 (MQ=255)

tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGcc                                                                                        >  1:1787584/1‑93 (MQ=255)

 ttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGccc                                                                                       <  1:1603950/93‑1 (MQ=255)

   ccGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTa                                                                                     <  1:3372108/93‑1 (MQ=255)

     ggcggcACCCCGCAAACCGGCGTCGGGTTGCCACGTTTGAAGTATTGATAAGAGAAACC‑AAACCGCCGCCCGGTGTACCAATCCGCCCTAACa                                                                                  <  2:6771800/93‑1 (MQ=255)

     ggCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAAc                                                                                   >  1:6513598/1‑93 (MQ=255)

     ggCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTCAc                                                                                   >  1:2864466/1‑93 (MQ=255)

      gcagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACa                                                                                  <  1:5056429/93‑1 (MQ=255)

        ctcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGATAAACCAAATCCGCCGCCCGGTGTACCAATCCTCCCTAACATc                                                                                <  2:4853586/91‑1 (MQ=255)

          caCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgc                                                                              <  2:2572795/93‑1 (MQ=255)

           aCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgcc                                                                             >  2:4151317/1‑93 (MQ=255)

              ccgccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCgccgcc                                                                          <  1:2553420/93‑1 (MQ=255)

              ccgccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCgccgcc                                                                          <  1:1908760/93‑1 (MQ=255)

              ccgccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCgccgcc                                                                          <  1:6043590/93‑1 (MQ=255)

                 ccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAACCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGc                                                                       <  1:3691191/93‑1 (MQ=255)

                 ccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGc                                                                       <  1:4908906/93‑1 (MQ=255)

                 ccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGc                                                                       <  2:1194421/93‑1 (MQ=255)

                   aaCACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGt                                                                     >  1:352598/1‑93 (MQ=255)

                      acGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGTCCGGTGTTCCAATCCGCCCTAACATCGCCGCCAGCGTCAc                                                                  >  2:614877/1‑93 (MQ=255)

                       cgcgTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCAcc                                                                 <  2:817558/93‑1 (MQ=255)

                          gTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGc                                                              >  2:203441/1‑93 (MQ=255)

                           tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  1:5162702/1‑93 (MQ=255)

                           tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAAACAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  2:7799811/1‑93 (MQ=255)

                           tCGGGTTGCCAACGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  1:1396837/1‑93 (MQ=255)

                            cGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCAt                                                            >  1:4280063/1‑93 (MQ=255)

                             gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           >  2:2685648/1‑93 (MQ=255)

                             gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           >  2:4574173/1‑93 (MQ=255)

                             gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           >  1:487475/1‑93 (MQ=255)

                              ggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATcc                                                          <  2:7249088/93‑1 (MQ=255)

                               gTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCa                                                         >  1:3572874/1‑93 (MQ=255)

                                 tGCCACCGTTGGAGTAATGATAAGAGAAACCAAACCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgt                                                       <  2:4855204/93‑1 (MQ=255)

                                 tGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgt                                                       <  2:4354710/93‑1 (MQ=255)

                                    cACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGtt                                                    >  2:1258562/1‑93 (MQ=255)

                                      ccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                                                  <  2:1698895/93‑1 (MQ=255)

                                      ccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                                                  >  2:2237068/1‑93 (MQ=255)

                                      ccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                                                  <  1:1331742/93‑1 (MQ=255)

                                       cGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCt                                                 >  2:6553441/1‑93 (MQ=255)

                                       cGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCt                                                 <  1:4474155/93‑1 (MQ=255)

                                        gTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                                                >  1:782093/1‑93 (MQ=255)

                                         ttGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTGCCAATCCGCCCTAACATCCCCGCCAGCGTCACCAGCATCCAGTGTTTCTGt                                               <  2:6688342/93‑1 (MQ=255)

                                         ttGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGt                                               >  1:212102/1‑93 (MQ=255)

                                         ttGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGt                                               >  1:7461461/1‑93 (MQ=255)

                                           ggAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTc                                             >  2:5898865/1‑93 (MQ=255)

                                               tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         >  2:5362987/1‑93 (MQ=255)

                                               tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         >  2:1967535/1‑93 (MQ=255)

                                               tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         >  2:4351244/1‑93 (MQ=255)

                                                aaTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCat                                        <  1:2313016/93‑1 (MQ=255)

                                                 aTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCata                                       <  1:3864867/93‑1 (MQ=255)

                                                 aTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGAGTTTCTGTTCACCata                                       >  1:7037893/1‑93 (MQ=255)

                                                  tGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCatat                                      >  2:3281180/1‑93 (MQ=255)

                                                  tGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCatat                                      >  2:1642570/1‑93 (MQ=255)

                                                     tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc                                   >  1:232765/1‑93 (MQ=255)

                                                     tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc                                   <  1:6840894/93‑1 (MQ=255)

                                                     tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc                                   >  1:6023759/1‑93 (MQ=255)

                                                      aaGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACAAGCATCCAGTGTTTCTGTTCGCCATATTGCt                                  <  2:5487293/93‑1 (MQ=255)

                                                       agagAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTg                                 <  1:5332258/93‑1 (MQ=255)

                                                       agagAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTg                                 <  2:2997779/93‑1 (MQ=255)

                                                        gataaaCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTgg                                <  1:5858851/90‑1 (MQ=255)

                                                         agaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc                               <  2:407505/93‑1 (MQ=255)

                                                          gaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGCTTCTGTTCACCATATTGCTGgcg                              >  1:2034694/1‑93 (MQ=255)

                                                               cAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa                         <  1:4407215/93‑1 (MQ=255)

                                                                aaaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAt                        <  1:937945/93‑1 (MQ=255)

                                                                aaaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAt                        <  1:276489/93‑1 (MQ=255)

                                                                 aaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAtt                       <  1:364360/93‑1 (MQ=255)

                                                                  aaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc                      <  1:2616502/93‑1 (MQ=255)

                                                                  aaCCGCCCCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCCTCCCCAGCCTCCGGTGTTTCTTTTCACCATATTGCTGGCGCTGAATTc                      <  2:5560081/93‑1 (MQ=255)

                                                                   aCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc                     >  1:1070440/1‑93 (MQ=255)

                                                                   aCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc                     >  2:6641142/1‑93 (MQ=255)

                                                                   aCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc                     >  1:7588699/1‑93 (MQ=255)

                                                                   aCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc                     <  1:4811462/93‑1 (MQ=255)

                                                                    ccgccgCCCGGTGTACCAATCCGTCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc                    <  2:5660070/93‑1 (MQ=255)

                                                                    ccgccgCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc                    >  1:5469356/1‑93 (MQ=255)

                                                                    ccgccgCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc                    <  2:4726110/93‑1 (MQ=255)

                                                                     cgccgcCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc                   <  2:4117836/93‑1 (MQ=255)

                                                                     cgccgcCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc                   <  2:5280396/93‑1 (MQ=255)

                                                                      gccgccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa                  <  1:654145/93‑1 (MQ=255)

                                                                      gccgccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa                  <  2:1369081/93‑1 (MQ=255)

                                                                      gccgccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa                  >  2:6304325/1‑93 (MQ=255)

                                                                       ccgccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg                 <  1:7900732/93‑1 (MQ=255)

                                                                         gccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGcc               <  1:5675128/93‑1 (MQ=255)

                                                                          cccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCt              <  2:7358794/93‑1 (MQ=255)

                                                                           ccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg             >  1:4411392/1‑93 (MQ=255)

                                                                           ccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg             <  2:7533896/93‑1 (MQ=255)

                                                                            cGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc            <  2:2534989/93‑1 (MQ=255)

                                                                            cGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc            >  1:1166817/1‑93 (MQ=255)

                                                                             ggTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGcc           >  2:6702375/1‑93 (MQ=255)

                                                                              gtgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa          <  1:7766969/93‑1 (MQ=255)

                                                                              gtgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa          >  2:3336137/1‑93 (MQ=255)

                                                                               tgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt         >  2:4332088/1‑93 (MQ=255)

                                                                                 tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAGTTCCCCAGCCTGCCATCa       >  1:4622713/1‑93 (MQ=255)

                                                                                 tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  2:5748705/93‑1 (MQ=255)

                                                                                 tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  2:5828577/93‑1 (MQ=255)

                                                                                 tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  1:1003645/93‑1 (MQ=255)

                                                                                  aCCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAg      <  1:2422516/93‑1 (MQ=255)

                                                                                   ccAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGc     >  1:3684906/1‑93 (MQ=255)

                                                                                   ccAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGc     >  2:3640808/1‑93 (MQ=255)

                                                                                     aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt   <  2:1792093/93‑1 (MQ=255)

                                                                                     aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt   >  1:1699394/1‑93 (MQ=255)

TTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953813‑1953991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  >  NC_000913/1953809‑1953995

GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCC                                                                                               <  SRR4302543.5750763/94‑1 (MQ=60)

GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCC                                                                                               <  SRR4302543.6627037/94‑1 (MQ=60)

   ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCC                                                                                            >  SRR4302543.663974/1‑94 (MQ=60)

   ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCC                                                                                            >  SRR4302543.2531198/1‑94 (MQ=60)

   ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCC                                                                                            >  SRR4302543.3146908/1‑94 (MQ=60)

   ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCC                                                                                            >  SRR4302543.1803573/1‑94 (MQ=60)

     TTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCT                                                                                          <  SRR4302543.1618268/94‑1 (MQ=60)

       CCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAA                                                                                        <  SRR4302543.3402392/94‑1 (MQ=60)

        CGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAAC                                                                                       >  SRR4302543.6574952/1‑94 (MQ=60)

        CGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTCAC                                                                                       >  SRR4302543.2890575/1‑94 (MQ=60)

          GCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAT                                                                                     <  SRR4302543.6591537/94‑1 (MQ=60)

          GCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAT                                                                                     <  SRR4302543.5102393/94‑1 (MQ=60)

          GCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAT                                                                                     >  SRR4302543.2133442/1‑94 (MQ=60)

           CAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATC                                                                                    >  SRR4302543.1519734/1‑94 (MQ=60)

             GCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGC                                                                                  <  SRR4302543.3518125/94‑1 (MQ=60)

             GCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGC                                                                                  >  SRR4302543.3024969/1‑94 (MQ=60)

              CACGCCGCCAACACGCGTCGGGTTGACACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCC                                                                                 <  SRR4302543.544060/94‑1 (MQ=60)

                  CCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCA                                                                             <  SRR4302543.6100794/94‑1 (MQ=60)

                  CCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCA                                                                             <  SRR4302543.1925956/94‑1 (MQ=60)

                  CCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCA                                                                             <  SRR4302543.2576755/94‑1 (MQ=60)

                  CCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCA                                                                             <  SRR4302543.1856628/94‑1 (MQ=60)

                    GCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGC                                                                           >  SRR4302543.35998/1‑94 (MQ=60)

                    GCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGC                                                                           >  SRR4302543.6078769/1‑94 (MQ=60)

                     CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAACCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                          <  SRR4302543.3723630/94‑1 (MQ=60)

                     CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                          <  SRR4302543.781407/94‑1 (MQ=60)

                     CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                          >  SRR4302543.5068355/1‑94 (MQ=60)

                     CCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCG                                                                          <  SRR4302543.4953647/94‑1 (MQ=60)

                      CAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGT                                                                         >  SRR4302543.2200276/1‑94 (MQ=60)

                      CAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGT                                                                         >  SRR4302543.355855/1‑94 (MQ=60)

                      CAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGT                                                                         >  SRR4302543.3721923/1‑94 (MQ=60)

                      CAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGC                                                                         >  SRR4302543.6360757/1‑94 (MQ=60)

                       AACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTC                                                                        <  SRR4302543.7535739/94‑1 (MQ=60)

                             CGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGC                                                                  >  SRR4302543.970632/1‑94 (MQ=60)

                              GTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCA                                                                 >  SRR4302543.5209354/1‑94 (MQ=60)

                              GTCGGGTTGCCAACGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCA                                                                 >  SRR4302543.1409436/1‑94 (MQ=60)

                               TCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCAT                                                                >  SRR4302543.4318964/1‑94 (MQ=60)

                                CGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCCCCAGCATC                                                               <  SRR4302543.5921742/94‑1 (MQ=60)

                                CGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATC                                                               >  SRR4302543.491981/1‑94 (MQ=60)

                                CGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATC                                                               >  SRR4302543.1147311/1‑94 (MQ=60)

                                  GGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCA                                                             >  SRR4302543.3604463/1‑94 (MQ=60)

                                  GGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCA                                                             >  SRR4302543.3604460/1‑94 (MQ=60)

                                   GTTGCCCCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTCCCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAG                                                            <  SRR4302543.6090379/94‑1 (MQ=60)

                                    TTGCCACCGTGGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGT                                                           <  SRR4302543.3822392/94‑1 (MQ=60)

                                       CCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTT                                                        >  SRR4302543.5727142/1‑94 (MQ=60)

                                       CCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTT                                                        >  SRR4302543.7890090/1‑94 (MQ=60)

                                        CACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTT                                                       <  SRR4302543.2644024/94‑1 (MQ=60)

                                        CACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTT                                                       >  SRR4302543.261054/1‑94 (MQ=60)

                                         ACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTC                                                      >  SRR4302543.7992181/1‑94 (MQ=60)

                                          CCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCT                                                     <  SRR4302543.1343612/94‑1 (MQ=60)

                                           CGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTG                                                    >  SRR4302543.788974/1‑94 (MQ=60)

                                           CGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTG                                                    <  SRR4302543.4514758/94‑1 (MQ=60)

                                            GTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGT                                                   >  SRR4302543.7532932/1‑94 (MQ=60)

                                            GTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGT                                                   >  SRR4302543.213913/1‑94 (MQ=60)

                                                 AGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACC                                              >  SRR4302543.7346491/1‑94 (MQ=60)

                                                  GTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCA                                             >  SRR4302543.528312/1‑94 (MQ=60)

                                                  GTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCA                                             <  SRR4302543.6612543/94‑1 (MQ=60)

                                                    AATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATA                                           <  SRR4302543.103951/94‑1 (MQ=60)

                                                    AATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATA                                           <  SRR4302543.2334055/94‑1 (MQ=60)

                                                    AATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGAGTTTCTGTTCACCATA                                           >  SRR4302543.7105743/1‑94 (MQ=60)

                                                     ATGATAAGAGAAGCCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAT                                          >  SRR4302543.5714940/1‑94 (MQ=60)

                                                     ATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAT                                          >  SRR4302543.1652811/1‑94 (MQ=60)

                                                     ATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAT                                          <  SRR4302543.3899006/94‑1 (MQ=60)

                                                     ATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAT                                          <  SRR4302543.233902/94‑1 (MQ=60)

                                                       GATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTG                                        >  SRR4302543.1178705/1‑94 (MQ=60)

                                                        ATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGC                                       >  SRR4302543.234772/1‑94 (MQ=60)

                                                        ATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGC                                       >  SRR4302543.2797061/1‑94 (MQ=60)

                                                        ATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGC                                       >  SRR4302543.6079747/1‑94 (MQ=60)

                                                         TAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCT                                      <  SRR4302543.3236287/94‑1 (MQ=60)

                                                         TAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCT                                      <  SRR4302543.6905461/94‑1 (MQ=60)

                                                          AAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTG                                     <  SRR4302543.4512884/94‑1 (MQ=60)

                                                           AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                    <  SRR4302543.4709573/94‑1 (MQ=60)

                                                           AGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGG                                    <  SRR4302543.5380479/94‑1 (MQ=60)

                                                            GATAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGC                                   <  SRR4302543.5912183/94‑1 (MQ=60)

                                                             AGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGCTTCTGTTCACCATATTGCTGGCG                                  >  SRR4302543.2052966/1‑94 (MQ=60)

                                                              GAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGC                                 >  SRR4302543.4893627/1‑94 (MQ=60)

                                                              GAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGC                                 >  SRR4302543.5124411/1‑94 (MQ=60)

                                                                 ACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGA                              >  SRR4302543.1377387/1‑94 (MQ=60)

                                                                   CAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAT                            <  SRR4302543.4447164/94‑1 (MQ=60)

                                                                   CAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAT                            >  SRR4302543.2382439/1‑94 (MQ=60)

                                                                   CAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAT                            <  SRR4302543.4447163/94‑1 (MQ=60)

                                                                    AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT                           >  SRR4302543.4196862/1‑94 (MQ=60)

                                                                    AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT                           <  SRR4302543.946140/94‑1 (MQ=60)

                                                                    AAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATT                           <  SRR4302543.278932/94‑1 (MQ=60)

                                                                     AAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC                          >  SRR4302543.6986308/1‑94 (MQ=60)

                                                                     AAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTC                          <  SRR4302543.367739/94‑1 (MQ=60)

                                                                      AACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCC                         <  SRR4302543.2640232/94‑1 (MQ=60)

                                                                      AACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCC                         >  SRR4302543.7660810/1‑94 (MQ=60)

                                                                      AACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCC                         >  SRR4302543.1080062/1‑94 (MQ=60)

                                                                       ACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCC                        >  SRR4302543.5518894/1‑94 (MQ=60)

                                                                       ACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCC                        <  SRR4302543.4855398/94‑1 (MQ=60)

                                                                          GCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAG                     <  SRR4302543.660131/94‑1 (MQ=60)

                                                                           CCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGC                    <  SRR4302543.7974998/94‑1 (MQ=60)

                                                                             GCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCT                  >  SRR4302543.3695731/1‑94 (MQ=60)

                                                                             GCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCT                  <  SRR4302543.5726931/94‑1 (MQ=60)

                                                                              CCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTG                 >  SRR4302543.4451375/1‑94 (MQ=60)

                                                                               CCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGC                >  SRR4302543.1177420/1‑94 (MQ=60)

                                                                                CGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCC               >  SRR4302543.4587312/1‑94 (MQ=60)

                                                                                  GTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAT             <  SRR4302543.7840252/94‑1 (MQ=60)

                                                                                    GTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAGTTCCCCAGCCTGCCATCA           >  SRR4302543.4664713/1‑94 (MQ=60)

                                                                                    GTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCA           >  SRR4302543.3534645/1‑94 (MQ=60)

                                                                                     TACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAG          <  SRR4302543.1012519/94‑1 (MQ=60)

                                                                                      ACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGC         <  SRR4302543.2444385/94‑1 (MQ=60)

                                                                                      ACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGC         >  SRR4302543.3717285/1‑94 (MQ=60)

                                                                                      ACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGC         >  SRR4302543.4448054/1‑94 (MQ=60)

                                                                                        CAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAT       >  SRR4302543.1796145/1‑94 (MQ=60)

                                                                                        CAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAT       >  SRR4302543.1714625/1‑94 (MQ=60)

                                                                                        CAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAT       >  SRR4302543.197208/1‑94 (MQ=60)

                                                                                         AATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT      >  SRR4302543.7917937/1‑94 (MQ=60)

                                                                                          ATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG     >  SRR4302543.6709172/1‑94 (MQ=60)

                                                                                          ATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG     <  SRR4302543.6646934/94‑1 (MQ=60)

                                                                                          ATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG     >  SRR4302543.2237132/1‑94 (MQ=60)

                                                                                          ATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG     >  SRR4302543.356322/1‑94 (MQ=60)

                                                                                          ATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG     >  SRR4302543.5129828/1‑94 (MQ=60)

                                                                                          ATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTG     <  SRR4302543.4923760/94‑1 (MQ=60)

                                                                                            CCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTG   <  SRR4302543.6644542/94‑1 (MQ=60)

                                                                                             CGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  <  SRR4302543.6944179/94‑1 (MQ=60)

GACATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGC  >  NC_000913/1953809‑1953995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`