Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,111,589 A→G M254T (ATG→ACG)  galF ← putative a UDP‑gal transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,111,5890AG100.0% 402.4 / NA 127M254T (ATG→ACG) galFputative a UDP‑gal transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (69/58);  total (69/58)

Only 100 of 129 total aligned reads displayed.

CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGT  >  NC_000913/2111499‑2111678
                                                                                          |                                                                                         
ccTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGtt                                                                                         >  2:1722827/1‑93 (MQ=255)
 cttcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTg                                                                                        >  2:937595/1‑93 (MQ=255)
 cttcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTg                                                                                        >  1:1214633/1‑93 (MQ=255)
  ttcttTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGc                                                                                       <  2:2014142/93‑1 (MQ=255)
     tttCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATc                                                                                    >  2:4509719/1‑93 (MQ=255)
       tCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCaa                                                                                  <  1:4039165/93‑1 (MQ=255)
       tCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCaa                                                                                  >  1:1776420/1‑93 (MQ=255)
         aGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACg                                                                                >  1:682425/1‑93 (MQ=255)
              gCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTg                                                                           <  1:2887228/93‑1 (MQ=255)
               cGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGt                                                                          <  2:4286722/93‑1 (MQ=255)
               cGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGt                                                                          >  1:635015/1‑93 (MQ=255)
               cGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGt                                                                          <  1:3518975/93‑1 (MQ=255)
                 tAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGttt                                                                        >  2:1963424/1‑93 (MQ=255)
                 tAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGttt                                                                        <  1:636975/93‑1 (MQ=255)
                  aGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGtttt                                                                       <  1:1510449/93‑1 (MQ=255)
                    gCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGtttttt                                                                     <  2:2081872/93‑1 (MQ=255)
                     ccATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCGACGGATTGTTTTTTc                                                                    >  2:3253442/1‑93 (MQ=255)
                      cATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCg                                                                   <  2:458617/93‑1 (MQ=255)
                       aTACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGc                                                                  <  1:4559844/93‑1 (MQ=255)
                        tACTTCACAAACGCCTGCATATAGCCTATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGcc                                                                 >  1:622789/1‑93 (MQ=255)
                        tACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGcc                                                                 >  1:72175/1‑93 (MQ=255)
                        tACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGcc                                                                 >  2:4335540/1‑93 (MQ=255)
                        tACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGcc                                                                 >  1:4040715/1‑93 (MQ=255)
                         aCTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCa                                                                >  1:3676106/1‑93 (MQ=255)
                         aCTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCa                                                                >  1:865273/1‑93 (MQ=255)
                          cTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAg                                                               >  2:4116937/1‑93 (MQ=255)
                              acaAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCg                                                           >  2:1625087/1‑93 (MQ=255)
                               caAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCgg                                                          >  2:3428606/1‑93 (MQ=255)
                               caAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCgg                                                          >  2:3946624/1‑93 (MQ=255)
                                 aaCGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGGCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCa                                                        >  1:3040586/1‑93 (MQ=255)
                                 aaCGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCa                                                        >  2:489523/1‑93 (MQ=255)
                                   cGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGGAAt                                                      <  1:4132817/93‑1 (MQ=255)
                                   cGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAAt                                                      >  1:1788409/1‑93 (MQ=255)
                                   cGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAAt                                                      <  2:2520945/93‑1 (MQ=255)
                                    gCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATa                                                     <  2:3628002/93‑1 (MQ=255)
                                     ccTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAg                                                    <  2:3240631/93‑1 (MQ=255)
                                     ccTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAg                                                    <  2:2245734/93‑1 (MQ=255)
                                      cTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGc                                                   >  2:110597/1‑93 (MQ=255)
                                      cTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGc                                                   >  2:4628751/1‑93 (MQ=255)
                                       tGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCa                                                  >  1:2246406/1‑93 (MQ=255)
                                            ataGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagt                                             <  2:3121502/93‑1 (MQ=255)
                                            ataGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagt                                             <  2:3995841/93‑1 (MQ=255)
                                            ataGCCCATTTTTTTCCCGCAGTCGTAACTGTCGCCGCTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagt                                             <  1:1192963/93‑1 (MQ=255)
                                             taGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtc                                            <  1:2752353/93‑1 (MQ=255)
                                             taGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtc                                            <  2:118066/93‑1 (MQ=255)
                                               gCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtcag                                          >  1:3405740/1‑93 (MQ=255)
                                               gCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCAtcagtcag                                          <  2:4264048/93‑1 (MQ=255)
                                                cccATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGc                                         >  2:1377301/1‑93 (MQ=255)
                                                  cATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTg                                       >  2:3953571/1‑93 (MQ=255)
                                                    tttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGaa                                     <  1:1740296/93‑1 (MQ=255)
                                                    tttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGaa                                     >  1:125453/1‑93 (MQ=255)
                                                    tttctttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGaa                                     >  1:827502/1‑93 (MQ=255)
                                                     ttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAt                                    <  1:4561533/93‑1 (MQ=255)
                                                     ttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAt                                    <  1:2755379/93‑1 (MQ=255)
                                                     ttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAt                                    <  1:2694029/93‑1 (MQ=255)
                                                     ttttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAt                                    <  2:2885053/93‑1 (MQ=255)
                                                      tttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATa                                   <  1:4530019/93‑1 (MQ=255)
                                                      tttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATa                                   <  2:3838567/93‑1 (MQ=255)
                                                      tttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATa                                   <  2:1065757/93‑1 (MQ=255)
                                                      tttttGCCGCAGTCGTAACTGACGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCGGAATa                                   <  2:2309039/93‑1 (MQ=255)
                                                       ttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATAc                                  <  1:2761753/93‑1 (MQ=255)
                                                       ttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATAc                                  <  2:2850299/93‑1 (MQ=255)
                                                       ttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATAc                                  <  2:4100806/93‑1 (MQ=255)
                                                       ttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATAc                                  <  1:2251594/93‑1 (MQ=255)
                                                       ttttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATAc                                  >  1:566377/1‑93 (MQ=255)
                                                        tttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACg                                 <  2:1576624/93‑1 (MQ=255)
                                                        tttGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACg                                 <  2:3475756/93‑1 (MQ=255)
                                                          tGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTc                               <  2:1676844/93‑1 (MQ=255)
                                                            ccGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGTATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTccc                             <  2:4149629/93‑1 (MQ=255)
                                                            ccGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTccc                             <  1:2396735/93‑1 (MQ=255)
                                                              gCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCa                           >  2:2028440/1‑93 (MQ=255)
                                                              gCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCa                           >  2:1074708/1‑93 (MQ=255)
                                                               cAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCac                          >  1:3649964/1‑92 (MQ=255)
                                                               cAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCac                          >  1:2776156/1‑92 (MQ=255)
                                                                    gTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAcc                     >  2:1832394/1‑93 (MQ=255)
                                                                     tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                    >  1:214191/1‑93 (MQ=255)
                                                                      aaCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                   <  1:1407237/93‑1 (MQ=255)
                                                                      aaCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAg                   <  1:235704/93‑1 (MQ=255)
                                                                       aCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAgg                  >  1:202619/1‑93 (MQ=255)
                                                                       aCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAgg                  >  1:1649080/1‑93 (MQ=255)
                                                                        cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                 <  1:937595/93‑1 (MQ=255)
                                                                        cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                 >  1:2777065/1‑93 (MQ=255)
                                                                        cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                 >  2:2969736/1‑93 (MQ=255)
                                                                        cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                 >  2:1818820/1‑93 (MQ=255)
                                                                         tGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCt                <  1:2909833/93‑1 (MQ=255)
                                                                          gTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTg               >  1:3577058/1‑93 (MQ=255)
                                                                           tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa              <  2:144938/93‑1 (MQ=255)
                                                                             gCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGt            >  1:625129/1‑93 (MQ=255)
                                                                                ggTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACg         >  2:615826/1‑93 (MQ=255)
                                                                                 gTCATCAGCGTTGCATCAACGTATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt        <  1:3019067/93‑1 (MQ=255)
                                                                                 gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt        <  2:4017741/93‑1 (MQ=255)
                                                                                 gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt        >  1:1505229/1‑93 (MQ=255)
                                                                                  tcatcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGtt       >  1:2144497/1‑93 (MQ=255)
                                                                                   catcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTc      >  2:203114/1‑93 (MQ=255)
                                                                                    atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc     <  1:3618487/93‑1 (MQ=255)
                                                                                     tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa    >  1:1171974/1‑93 (MQ=255)
                                                                                     tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa    >  1:4458114/1‑93 (MQ=255)
                                                                                      caGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAg   <  2:4239063/93‑1 (MQ=255)
                                                                                       aGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGt  >  2:1437818/1‑93 (MQ=255)
                                                                                       aGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGt  <  1:3428962/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGT  >  NC_000913/2111499‑2111678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCG  >  NC_000913/2111495‑2111682
                                                                                              |                                                                                             
CGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCA‑TTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCG                                                                                               >  SRR4302535.1253850/1‑94 (MQ=60)
  CCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGT                                                                                              <  SRR4302535.1308884/94‑1 (MQ=60)
   CCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTT                                                                                             <  SRR4302535.1167580/94‑1 (MQ=60)
    CCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTG                                                                                            >  SRR4302535.1223344/1‑94 (MQ=60)
         TTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCA                                                                                       <  SRR4302535.2206662/94‑1 (MQ=60)
          TTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAA                                                                                      <  SRR4302535.328936/94‑1 (MQ=60)
          TTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAA                                                                                      >  SRR4302535.1789282/1‑94 (MQ=60)
           TCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAAC                                                                                     <  SRR4302535.4066814/94‑1 (MQ=60)
            CAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACG                                                                                    >  SRR4302535.687202/1‑94 (MQ=60)
             AGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGG                                                                                   <  SRR4302535.63360/94‑1 (MQ=60)
                  GCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGT                                                                              <  SRR4302535.2907656/94‑1 (MQ=60)
                  GCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGT                                                                              >  SRR4302535.639438/1‑94 (MQ=60)
                   CGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTT                                                                             <  SRR4302535.3543420/94‑1 (MQ=60)
                     TAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTT                                                                           <  SRR4302535.641418/94‑1 (MQ=60)
                      AGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTT                                                                          <  SRR4302535.1521332/94‑1 (MQ=60)
                        GCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTC                                                                        >  SRR4302535.2922071/1‑94 (MQ=60)
                         CCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCG                                                                       >  SRR4302535.546393/1‑94 (MQ=60)
                           ATACTTCACAAACGCCTGCATATAGCCTATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCC                                                                     >  SRR4302535.627116/1‑94 (MQ=60)
                           ATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCC                                                                     >  SRR4302535.72701/1‑94 (MQ=60)
                           ATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCC                                                                     >  SRR4302535.4068378/1‑94 (MQ=60)
                           ATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCC                                                                     <  SRR4302535.4590970/94‑1 (MQ=60)
                            TACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCA                                                                    >  SRR4302535.871387/1‑94 (MQ=60)
                            TACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCA                                                                    >  SRR4302535.2775343/1‑94 (MQ=60)
                            TACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCA                                                                    >  SRR4302535.3701557/1‑94 (MQ=60)
                                 CACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCG                                                               <  SRR4302535.1591525/94‑1 (MQ=60)
                                    AAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGGCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCA                                                            >  SRR4302535.3061885/1‑94 (MQ=60)
                                      ACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAAT                                                          >  SRR4302535.1801348/1‑94 (MQ=60)
                                       CGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGGAATA                                                         <  SRR4302535.4161092/94‑1 (MQ=60)
                                         CCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGC                                                       >  SRR4302535.735500/1‑94 (MQ=60)
                                          CTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCA                                                      >  SRR4302535.2262378/1‑94 (MQ=60)
                                                ATAGCCCATTTTTTTCCCGCAGTCGTAACTGTCGCCGCTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTC                                                <  SRR4302535.1201512/94‑1 (MQ=60)
                                                 TAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCA                                               <  SRR4302535.2771968/94‑1 (MQ=60)
                                                  AGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAG                                              >  SRR4302535.3429442/1‑94 (MQ=60)
                                                     CCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTG                                           >  SRR4302535.3970831/1‑94 (MQ=60)
                                                       ATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAA                                         >  SRR4302535.126335/1‑94 (MQ=60)
                                                       ATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAA                                         >  SRR4302535.3640560/1‑94 (MQ=60)
                                                       ATTTCTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAA                                         >  SRR4302535.833296/1‑94 (MQ=60)
                                                        TTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAAT                                        <  SRR4302535.1752873/94‑1 (MQ=60)
                                                         TTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATA                                       <  SRR4302535.2775019/94‑1 (MQ=60)
                                                         TTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATA                                       <  SRR4302535.2713281/94‑1 (MQ=60)
                                                         TTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATA                                       <  SRR4302535.4592664/94‑1 (MQ=60)
                                                          TTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATAC                                      <  SRR4302535.4561032/94‑1 (MQ=60)
                                                          TTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATAC                                      >  SRR4302535.570341/1‑94 (MQ=60)
                                                           TTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACG                                     <  SRR4302535.2781435/94‑1 (MQ=60)
                                                           TTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACG                                     >  SRR4302535.1860991/1‑94 (MQ=60)
                                                           TTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACG                                     <  SRR4302535.2267628/94‑1 (MQ=60)
                                                                CCGCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCC                                <  SRR4302535.2413960/94‑1 (MQ=60)
                                                                  GCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCAC                              >  SRR4302535.2795921/1‑94 (MQ=60)
                                                                  GCAGTCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCAC                              >  SRR4302535.3675236/1‑94 (MQ=60)
                                                                        GTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCA                        >  SRR4302535.215658/1‑94 (MQ=60)
                                                                          AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                      >  SRR4302535.204000/1‑94 (MQ=60)
                                                                          AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                      >  SRR4302535.1661009/1‑94 (MQ=60)
                                                                          AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                      <  SRR4302535.1417290/94‑1 (MQ=60)
                                                                          AACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGG                      <  SRR4302535.237333/94‑1 (MQ=60)
                                                                           ACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGC                     >  SRR4302535.2796835/1‑94 (MQ=60)
                                                                            CTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCT                    <  SRR4302535.944235/94‑1 (MQ=60)
                                                                             TGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTG                   <  SRR4302535.2930391/94‑1 (MQ=60)
                                                                             TGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTG                   >  SRR4302535.3601856/1‑94 (MQ=60)
                                                                                CGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGT                >  SRR4302535.629477/1‑94 (MQ=60)
                                                                                    GGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGT            >  SRR4302535.1516050/1‑94 (MQ=60)
                                                                                     GTCATCAGCGTTGCATCAACGTATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTT           <  SRR4302535.3040213/94‑1 (MQ=60)
                                                                                     GTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTT           >  SRR4302535.2159924/1‑94 (MQ=60)
                                                                                       CATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCC         >  SRR4302535.4274319/1‑94 (MQ=60)
                                                                                        ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA        <  SRR4302535.3643572/94‑1 (MQ=60)
                                                                                        ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA        >  SRR4302535.1303848/1‑94 (MQ=60)
                                                                                        ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA        >  SRR4302535.1180344/1‑94 (MQ=60)
                                                                                        ATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCA        >  SRR4302535.4488689/1‑94 (MQ=60)
                                                                                           AGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTT     <  SRR4302535.2464221/94‑1 (MQ=60)
                                                                                           AGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTT     <  SRR4302535.3452798/94‑1 (MQ=60)
                                                                                             CGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCC   >  SRR4302535.949748/1‑94 (MQ=60)
                                                                                             CGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCC   >  SRR4302535.2581076/1‑94 (MQ=60)
                                                                                              GTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCG  >  SRR4302535.1170861/1‑94 (MQ=60)
                                                                                              |                                                                                             
CGCCCCTTCTTTCAGGTTGCGTAGGCCATACTTCACAAACGCCTGCATATAGCCCATTTTTTTGCCGCAGTCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCG  >  NC_000913/2111495‑2111682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑