Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,113,625	T→C	L98L (TTA→TTG)	wcaM ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,113,625	0	T	C	100.0%	529.9 / NA	163	L98L (TTA→TTG)	wcaM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (74/89); total (74/89)

Only 100 of 170 total aligned reads displayed.

CCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATT  >  NC_000913/2113535‑2113714

cccTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCaa                                                                                         >  1:2633664/1‑93 (MQ=255)

  cTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGa                                                                                       >  1:2817802/1‑93 (MQ=255)

      gATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACaaa                                                                                   <  1:3827512/93‑1 (MQ=255)

      gATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACaaa                                                                                   <  1:2364763/93‑1 (MQ=255)

      gATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACaaa                                                                                   <  1:1486911/93‑1 (MQ=255)

       aTGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAAt                                                                                  <  1:1348610/93‑1 (MQ=255)

        tGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATc                                                                                 >  1:810505/1‑93 (MQ=255)

        tGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATc                                                                                 <  2:3667707/93‑1 (MQ=255)

          gTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGc                                                                               >  2:1996413/1‑93 (MQ=255)

           tGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGcc                                                                              <  1:3036129/93‑1 (MQ=255)

               gTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCctct                                                                          <  1:3029899/93‑1 (MQ=255)

                tCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTg                                                                         <  2:4178744/93‑1 (MQ=255)

                  aGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGcc                                                                       >  2:3502022/1‑93 (MQ=255)

                   gAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCg                                                                      >  2:2996713/1‑93 (MQ=255)

                     aCCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGtg                                                                    >  2:2347182/1‑92 (MQ=255)

                       cACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGc                                                                  <  1:1668780/93‑1 (MQ=255)

                       cACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGc                                                                  <  1:4649968/93‑1 (MQ=255)

                        aCGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGcc                                                                 >  1:3090259/1‑93 (MQ=255)

                            acaTCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCgcgc                                                             >  1:3276584/1‑93 (MQ=255)

                            acaTCCAGGGGGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCgcgc                                                             >  1:3258645/1‑93 (MQ=255)

                              aTCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCaa                                                           >  1:381014/1‑93 (MQ=255)

                              aTCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCaa                                                           <  1:3992465/93‑1 (MQ=255)

                              aTCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCaa                                                           >  2:1346807/1‑93 (MQ=255)

                                 cAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCt                                                        >  2:4097236/1‑93 (MQ=255)

                                 cAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACATGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCt                                                        >  1:2515157/1‑93 (MQ=255)

                                  aGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCtt                                                       >  1:570655/1‑93 (MQ=255)

                                  aGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCtt                                                       <  2:1642325/93‑1 (MQ=255)

                                  aGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCtt                                                       >  2:1417375/1‑93 (MQ=255)

                                   gggTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTc                                                      <  2:2707957/93‑1 (MQ=255)

                                    ggTGATGTTATGCATACTGCCGCCATTCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTcc                                                     <  2:3794600/93‑1 (MQ=255)

                                     gTGATGTTATGCATACTGCCGCCATGCTCCCCTGTCAACTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCAGTTGCCGCGCAAGCTTCCa                                                    >  2:294757/1‑93 (MQ=255)

                                      tGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCaa                                                   >  2:1712400/1‑93 (MQ=255)

                                      gcaTGTTATGCACACCGCCGCCATCCTCCCCTGTCGCCTGGCTGCCGTCCTGCAAGACAAACCGCCCTCTGCCGTTGCAGCGCAAGCTTCCaa                                                   <  2:1903353/91‑1 (MQ=255)

                                         tGTTATGCATCCTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGaa                                                <  2:175385/93‑1 (MQ=255)

                                         tGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGaa                                                <  2:1835425/93‑1 (MQ=255)

                                          tttATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAAt                                               <  1:416682/92‑1 (MQ=255)

                                          gTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAAt                                               >  2:1961460/1‑93 (MQ=255)

                                            tATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAAtgt                                             <  1:4189428/93‑1 (MQ=255)

                                            tATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAAtgt                                             >  1:3190650/1‑93 (MQ=255)

                                            tATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAAtgt                                             <  1:2938404/93‑1 (MQ=255)

                                              tGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATgtgt                                           >  2:3187728/1‑93 (MQ=255)

                                              tGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATgtgt                                           >  2:4259070/1‑93 (MQ=255)

                                               gCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTa                                          >  2:1544978/1‑93 (MQ=255)

                                               gCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTa                                          >  1:1614513/1‑93 (MQ=255)

                                                cATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTaa                                         >  2:3419752/1‑93 (MQ=255)

                                                cATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTaa                                         <  2:4002101/93‑1 (MQ=255)

                                                cATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTaa                                         <  1:1522037/93‑1 (MQ=255)

                                                 aTACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAAc                                        <  2:3071379/93‑1 (MQ=255)

                                                 aTACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAAc                                        >  1:1768519/1‑93 (MQ=255)

                                                  tACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACg                                       >  2:4629279/1‑93 (MQ=255)

                                                   aCTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGt                                      <  2:2810583/93‑1 (MQ=255)

                                                   aCTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGt                                      <  2:895375/93‑1 (MQ=255)

                                                    cTGCCGCGATCCTCTCCTGTCACCTGGCTGCCGTCCTGCAAGAGAAATCGCCCTCTGCGGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGtt                                     <  2:3147256/93‑1 (MQ=255)

                                                    cTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGtt                                     <  1:4621325/93‑1 (MQ=255)

                                                      gccgccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGtttt                                   <  1:2756073/93‑1 (MQ=255)

                                                       ccgccATCCTCCCCTGTCCCCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTa                                  <  2:3357707/93‑1 (MQ=255)

                                                        cgccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTAc                                 <  1:4265568/93‑1 (MQ=255)

                                                        cgccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTAc                                 <  1:4429780/93‑1 (MQ=255)

                                                        cgccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTAc                                 <  1:1704842/93‑1 (MQ=255)

                                                        ccccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTAc                                 <  1:480633/91‑1 (MQ=255)

                                                         gccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTAcc                                <  2:2760175/93‑1 (MQ=255)

                                                         gccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTAcc                                >  1:4016967/1‑93 (MQ=255)

                                                          ccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCg                               <  1:2801713/93‑1 (MQ=255)

                                                          ccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCg                               >  1:1305127/1‑93 (MQ=255)

                                                          ccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCg                               >  2:283837/1‑93 (MQ=255)

                                                          ccATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCg                               <  1:4216775/93‑1 (MQ=255)

                                                           cATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCgg                              >  2:3923339/1‑93 (MQ=255)

                                                           cATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCgg                              >  2:591948/1‑93 (MQ=255)

                                                            aTCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCggg                             >  1:3604052/1‑93 (MQ=255)

                                                             tcctccCCTGTCCCCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCggga                            <  1:898789/93‑1 (MQ=255)

                                                             tcctccCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCggga                            <  2:1976159/93‑1 (MQ=255)

                                                             tcctccCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCggga                            >  1:2804942/1‑93 (MQ=255)

                                                               ctccCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCgggagg                          >  1:3208479/1‑93 (MQ=255)

                                                                tccCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCgggaggg                         >  1:3738007/1‑93 (MQ=255)

                                                                tccCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCgggaggg                         >  1:99340/1‑93 (MQ=255)

                                                                  cccTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGAt                       <  2:1210419/93‑1 (MQ=255)

                                                                   ccTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATa                      >  1:3641973/1‑93 (MQ=255)

                                                                    cTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATaa                     >  2:2598544/1‑93 (MQ=255)

                                                                    cTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATaa                     >  2:3953722/1‑93 (MQ=255)

                                                                     tGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATaaa                    <  1:2441758/93‑1 (MQ=255)

                                                                      gTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAg                   >  2:2670380/1‑93 (MQ=255)

                                                                       tCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGa                  <  2:1671425/93‑1 (MQ=255)

                                                                       tCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGa                  >  1:799835/1‑93 (MQ=255)

                                                                        cACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGAt                 <  1:1881676/93‑1 (MQ=255)

                                                                         aCCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGAtc                >  1:836318/1‑92 (MQ=255)

                                                                         aCCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATg                <  2:19072/93‑1 (MQ=255)

                                                                             ggCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCgg            <  1:1285997/93‑1 (MQ=255)

                                                                               cTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGgtg          <  1:3885422/93‑1 (MQ=255)

                                                                                  ccGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTg       <  2:1745641/93‑1 (MQ=255)

                                                                                   cGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGa      <  1:2281162/93‑1 (MQ=255)

                                                                                   cGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGa      >  1:760843/1‑93 (MQ=255)

                                                                                    gTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGat     >  2:3492300/1‑93 (MQ=255)

                                                                                    gTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGat     >  2:3330042/1‑93 (MQ=255)

                                                                                    gTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGat     >  1:1672601/1‑93 (MQ=255)

                                                                                     tCCTGCAGGACAAACCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGata    <  1:644512/93‑1 (MQ=255)

                                                                                     tCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGata    >  1:2117020/1‑93 (MQ=255)

                                                                                     tCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGata    <  2:1342749/93‑1 (MQ=255)

                                                                                      ccTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATTAAGATGCCGGTGTTGatat   <  2:4563621/93‑1 (MQ=255)

                                                                                      ccTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGatat   <  2:1144966/93‑1 (MQ=255)

                                                                                       cTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATAtt  <  1:1722218/93‑1 (MQ=255)

CCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATT  >  NC_000913/2113535‑2113714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTGTCA  >  NC_000913/2113534‑2113718

GCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAA                                                                                             <  SRR4302535.3420409/94‑1 (MQ=60)

GCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAA                                                                                             <  SRR4302535.274229/94‑1 (MQ=60)

GCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAA                                                                                             >  SRR4302535.2652537/1‑94 (MQ=60)

  CCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGA                                                                                           >  SRR4302535.2837818/1‑94 (MQ=60)

   CTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGAC                                                                                          >  SRR4302535.4215045/1‑94 (MQ=60)

       GATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAAT                                                                                      <  SRR4302535.3853915/94‑1 (MQ=60)

       GATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAAT                                                                                      <  SRR4302535.1497558/94‑1 (MQ=60)

       GATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAAT                                                                                      <  SRR4302535.2381741/94‑1 (MQ=60)

        ATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATC                                                                                     <  SRR4302535.4521054/94‑1 (MQ=60)

        ATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATC                                                                                     >  SRR4302535.3063478/1‑94 (MQ=60)

        ATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATC                                                                                     >  SRR4302535.816172/1‑94 (MQ=60)

        ATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATC                                                                                     <  SRR4302535.1358241/94‑1 (MQ=60)

          GGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGC                                                                                   >  SRR4302535.2972017/1‑94 (MQ=60)

            TGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCC                                                                                 <  SRR4302535.1346709/94‑1 (MQ=60)

            TGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCC                                                                                 <  SRR4302535.3057389/94‑1 (MQ=60)

            TGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCC                                                                                 >  SRR4302535.2294687/1‑94 (MQ=60)

            TGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCC                                                                                 >  SRR4302535.801410/1‑94 (MQ=60)

               AGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCTGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCT                                                                              <  SRR4302535.484033/94‑1 (MQ=60)

               AGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCT                                                                              <  SRR4302535.1717281/94‑1 (MQ=60)

                GTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTG                                                                             <  SRR4302535.436589/94‑1 (MQ=60)

                GTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTG                                                                             <  SRR4302535.3051109/94‑1 (MQ=60)

                        CACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCC                                                                     <  SRR4302535.1680855/94‑1 (MQ=60)

                        CACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCC                                                                     <  SRR4302535.3291789/94‑1 (MQ=60)

                        CACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCC                                                                     >  SRR4302535.3111781/1‑94 (MQ=60)

                        CACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCC                                                                     <  SRR4302535.4681460/94‑1 (MQ=60)

                         ACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCG                                                                    <  SRR4302535.3716325/94‑1 (MQ=60)

                           GCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCG                                                                  <  SRR4302535.197366/94‑1 (MQ=60)

                            CACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGC                                                                 >  SRR4302535.3299345/1‑94 (MQ=60)

                            CACATCCAGGGGGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGC                                                                 >  SRR4302535.3281292/1‑94 (MQ=60)

                              CATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAA                                                               >  SRR4302535.383649/1‑94 (MQ=60)

                               ATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAG                                                              <  SRR4302535.4019775/94‑1 (MQ=60)

                                TCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGC                                                             >  SRR4302535.1597993/1‑94 (MQ=60)

                                 CCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACATGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCT                                                            >  SRR4302535.2533289/1‑94 (MQ=60)

                                  CAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTT                                                           >  SRR4302535.3802244/1‑94 (MQ=60)

                                  CAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTT                                                           >  SRR4302535.574662/1‑94 (MQ=60)

                                       TGATGTTATGCATACTGCCGCCATCCTCTCCTGGCACCTGGCTGCAGTCCAGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAG                                                      <  SRR4302535.2539909/94‑1 (MQ=60)

                                           TTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATG                                                  <  SRR4302535.419601/94‑1 (MQ=60)

                                            TTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGT                                                 >  SRR4302535.3212716/1‑94 (MQ=60)

                                             TATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTG                                                <  SRR4302535.2959072/94‑1 (MQ=60)

                                             TATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTG                                                <  SRR4302535.4218104/94‑1 (MQ=60)

                                               TGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTA                                              >  SRR4302535.1626149/1‑94 (MQ=60)

                                                GCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAA                                             <  SRR4302535.2819830/94‑1 (MQ=60)

                                                GCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAA                                             <  SRR4302535.746739/94‑1 (MQ=60)

                                                 CATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAAC                                            <  SRR4302535.1532999/94‑1 (MQ=60)

                                                 CATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAAC                                            >  SRR4302535.1781341/1‑94 (MQ=60)

                                                   TACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGT                                          <  SRR4302535.815749/94‑1 (MQ=60)

                                                    ACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTT                                         <  SRR4302535.3962935/94‑1 (MQ=60)

                                                     CTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTT                                        <  SRR4302535.4652722/94‑1 (MQ=60)

                                                       GCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTA                                      <  SRR4302535.2775717/94‑1 (MQ=60)

                                                         CGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACC                                    <  SRR4302535.1675862/94‑1 (MQ=60)

                                                         CGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACC                                    <  SRR4302535.4294779/94‑1 (MQ=60)

                                                         CGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACC                                    <  SRR4302535.1717166/94‑1 (MQ=60)

                                                         CGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACC                                    <  SRR4302535.4460131/94‑1 (MQ=60)

                                                         CGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACC                                    >  SRR4302535.4044439/1‑94 (MQ=60)

                                                         CCCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACC                                    <  SRR4302535.484003/94‑1 (MQ=60)

                                                          GCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCG                                   >  SRR4302535.1314443/1‑94 (MQ=60)

                                                           CCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGG                                  <  SRR4302535.4245630/94‑1 (MQ=60)

                                                           CCATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGG                                  <  SRR4302535.2821641/94‑1 (MQ=60)

                                                            CATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGG                                 >  SRR4302535.3629059/1‑94 (MQ=60)

                                                             ATCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGA                                >  SRR4302535.2824887/1‑94 (MQ=60)

                                                              TCCTCCCCTGTCCCCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAG                               <  SRR4302535.905158/94‑1 (MQ=60)

                                                              TCCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAG                               <  SRR4302535.469680/94‑1 (MQ=60)

                                                               CCTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGG                              >  SRR4302535.3230702/1‑94 (MQ=60)

                                                                CTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGG                             >  SRR4302535.3763878/1‑94 (MQ=60)

                                                                CTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGG                             >  SRR4302535.2908390/1‑94 (MQ=60)

                                                                CTCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGG                             >  SRR4302535.100039/1‑94 (MQ=60)

                                                                 TCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGA                            <  SRR4302535.4178580/94‑1 (MQ=60)

                                                                 TCCCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGA                            <  SRR4302535.171461/94‑1 (MQ=60)

                                                                   CCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATA                          >  SRR4302535.3667197/1‑94 (MQ=60)

                                                                   CCCTGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATA                          >  SRR4302535.35465/1‑94 (MQ=60)

                                                                      TGTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAG                       <  SRR4302535.2459348/94‑1 (MQ=60)

                                                                       GTCACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGA                      >  SRR4302535.805452/1‑94 (MQ=60)

                                                                        TCACCTGGCTGCCGTCATGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGAT                     <  SRR4302535.175127/94‑1 (MQ=60)

                                                                         CACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATG                    <  SRR4302535.1895239/94‑1 (MQ=60)

                                                                         CACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATC                    >  SRR4302535.842184/1‑94 (MQ=60)

                                                                          ACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGC                   <  SRR4302535.902086/94‑1 (MQ=60)

                                                                          ACCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGC                   >  SRR4302535.2425121/1‑94 (MQ=60)

                                                                           CCTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCC                  >  SRR4302535.4237103/1‑94 (MQ=60)

                                                                            CTGGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCG                 >  SRR4302535.2762139/1‑94 (MQ=60)

                                                                             TGGATGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGG                >  SRR4302535.2823414/1‑94 (MQ=60)

                                                                              GGCTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGT               <  SRR4302535.1295164/94‑1 (MQ=60)

                                                                                CTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGT             <  SRR4302535.3912073/94‑1 (MQ=60)

                                                                                CTGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGT             >  SRR4302535.3208741/1‑94 (MQ=60)

                                                                                 TGCCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTT            >  SRR4302535.3911790/1‑94 (MQ=60)

                                                                                   CCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGA          >  SRR4302535.928664/1‑94 (MQ=60)

                                                                                   CCGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGA          >  SRR4302535.766205/1‑94 (MQ=60)

                                                                                    CGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGAT         <  SRR4302535.3266285/94‑1 (MQ=60)

                                                                                    CGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGAT         <  SRR4302535.2297456/94‑1 (MQ=60)

                                                                                    CGTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGAT         >  SRR4302535.1684708/1‑94 (MQ=60)

                                                                                     GTCCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATA        >  SRR4302535.2132292/1‑94 (MQ=60)

                                                                                      TCCTGCAGGACAAACCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATAT       <  SRR4302535.649009/94‑1 (MQ=60)

                                                                                       CCTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATT      >  SRR4302535.2463580/1‑94 (MQ=60)

                                                                                        CTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTG     >  SRR4302535.3451307/1‑94 (MQ=60)

                                                                                        CTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTG     <  SRR4302535.1734674/94‑1 (MQ=60)

                                                                                        CTGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTG     <  SRR4302535.1429377/94‑1 (MQ=60)

                                                                                         TGCAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTGT    <  SRR4302535.1924325/94‑1 (MQ=60)

                                                                                           CAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTGTCA  <  SRR4302535.1348007/94‑1 (MQ=60)

                                                                                           CAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTGTCA  >  SRR4302535.3514655/1‑94 (MQ=60)

GCCCTTTGATGGTGCAGTCAGAACCACGCACATCCAGGGTGATGTTATGCATACTGCCGCCATCCTCCCCTGTCACCTGGCTGCCGTCCTGTAAGACAAATCGCCCTCTGCCGTTGCCGCGCAAGCTTCCAAGAATGTGTAACGTTTTACCGGGAGGGATAAAGATGCCGGTGTTGATATTGTCA  >  NC_000913/2113534‑2113718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`