Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,123,741	A→G	Y10H (TAC→CAC)	wcaI ←	putative colanic biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,123,741	0	A	G	100.0%	430.0 / NA	136	Y10H (TAC→CAC)	wcaI	putative colanic biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (51/85); total (51/85)

Only 100 of 142 total aligned reads displayed.

GGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTAGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATT  >  NC_000913/2123652‑2123830

ggtAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGtt                                                                                        >  2:388777/1‑93 (MQ=255)

 gtAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGGTGCCGGTTAACTCCGGCGAGTGGTTa                                                                                       >  1:1278868/1‑93 (MQ=255)

 gtAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTa                                                                                       >  1:4231611/1‑93 (MQ=255)

  tAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTaa                                                                                      <  1:1107957/93‑1 (MQ=255)

  tAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTaa                                                                                      <  2:683251/93‑1 (MQ=255)

  tAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTaa                                                                                      >  2:1238598/1‑93 (MQ=255)

  tAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTaa                                                                                      <  1:905582/93‑1 (MQ=255)

   aaTGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAAt                                                                                     <  1:4619013/93‑1 (MQ=255)

   aaTGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGCATTTGCCGATGCTGGCTAATTCCGGCGAGTGGTTAAt                                                                                     >  1:4242142/1‑93 (MQ=255)

   aaTGACCCGCACCTCATGACCTTGTACCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAAt                                                                                     <  1:796502/93‑1 (MQ=255)

     agACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGc                                                                                   <  1:3915943/92‑1 (MQ=255)

                 cATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTa                                                                       <  1:4399607/93‑1 (MQ=255)

                 cATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTa                                                                       <  2:1160679/93‑1 (MQ=255)

                  aTGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTAt                                                                      >  2:1426124/1‑93 (MQ=255)

                  aTGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTAt                                                                      <  2:2309040/93‑1 (MQ=255)

                   tGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTAtt                                                                     <  1:2661573/93‑1 (MQ=255)

                      ccTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTc                                                                  >  1:2477882/1‑93 (MQ=255)

                       cTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCa                                                                 <  2:1495077/93‑1 (MQ=255)

                        ttGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCAt                                                                <  1:1072909/93‑1 (MQ=255)

                        ttGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCAt                                                                >  2:1804516/1‑93 (MQ=255)

                        ttGTCCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCAt                                                                <  2:4500778/93‑1 (MQ=255)

                         tgtgCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATa                                                               <  1:459603/93‑1 (MQ=255)

                          gtgCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATaa                                                              <  2:2507950/93‑1 (MQ=255)

                          gtgCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATaa                                                              <  1:1647771/93‑1 (MQ=255)

                           tgCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAAt                                                             <  1:277531/93‑1 (MQ=255)

                            gccgccAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATc                                                            <  1:2922153/93‑1 (MQ=255)

                            gccgccAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATc                                                            <  1:1597812/93‑1 (MQ=255)

                             ccgccAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTGTTTTCATAATcc                                                           <  2:3759468/93‑1 (MQ=255)

                               ctgagcgaTTCGCCCGTCGCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCgg                                                         <  1:581397/90‑1 (MQ=255)

                                ccagccaTTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCggg                                                        <  2:177346/93‑1 (MQ=255)

                                  agccaTTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTa                                                      <  1:3065790/93‑1 (MQ=255)

                                   gccaTTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTAc                                                     >  1:2361541/1‑93 (MQ=255)

                                     caTTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTc                                                   >  2:3197381/1‑93 (MQ=255)

                                     caTTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTc                                                   <  1:1017319/93‑1 (MQ=255)

                                      aTTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTcc                                                  >  1:4394574/1‑93 (MQ=255)

                                      aTTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTcc                                                  >  1:212759/1‑93 (MQ=255)

                                       ttCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCg                                                 >  1:1178462/1‑93 (MQ=255)

                                          caccaTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTa                                              <  1:4618103/93‑1 (MQ=255)

                                           accaTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTAc                                             >  2:2150813/1‑93 (MQ=255)

                                            ccaTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACg                                            >  1:1775753/1‑93 (MQ=255)

                                            ccaTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACg                                            >  2:658259/1‑93 (MQ=255)

                                            ccaTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACg                                            >  1:2128189/1‑93 (MQ=255)

                                             caTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGc                                           <  2:3373542/93‑1 (MQ=255)

                                             caTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGc                                           <  2:2884473/93‑1 (MQ=255)

                                             caTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGc                                           >  1:2659791/1‑93 (MQ=255)

                                              aTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCt                                          <  1:1662674/93‑1 (MQ=255)

                                              aTCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGCCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGCACTCCGGTACGCt                                          >  1:800558/1‑93 (MQ=255)

                                               tctcGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCtt                                         <  2:190995/93‑1 (MQ=255)

                                                ctcGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTtc                                        <  1:3511384/93‑1 (MQ=255)

                                                ctcGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTtc                                        <  2:4213397/93‑1 (MQ=255)

                                                ctcGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTtc                                        <  1:2955823/93‑1 (MQ=255)

                                                 tcGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTtct                                       >  1:776846/1‑93 (MQ=255)

                                                 tcGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTtct                                       >  2:4315934/1‑93 (MQ=255)

                                                 tcGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTtct                                       <  1:35905/93‑1 (MQ=255)

                                                   gCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCa                                     <  1:3197328/93‑1 (MQ=255)

                                                    ccGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAg                                    >  2:902093/1‑93 (MQ=255)

                                                    ccGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAg                                    >  1:1285620/1‑93 (MQ=255)

                                                      ggTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCg                                  >  2:4293453/1‑93 (MQ=255)

                                                      ggTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCg                                  >  2:1262970/1‑93 (MQ=255)

                                                       gtgtATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCga                                 <  2:3670875/93‑1 (MQ=255)

                                                       gtgtATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCga                                 <  2:288580/93‑1 (MQ=255)

                                                        tgtATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCgag                                >  2:2989821/1‑93 (MQ=255)

                                                        tgtATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCgag                                >  2:2542109/1‑93 (MQ=255)

                                                        ggtATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCgag                                >  1:3092503/2‑93 (MQ=255)

                                                         gtATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCgaga                               >  2:2640939/1‑93 (MQ=255)

                                                         gtATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCgaga                               <  2:2823737/93‑1 (MQ=255)

                                                          tATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGaa                              <  1:4548293/93‑1 (MQ=255)

                                                          tATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGaa                              >  1:4508095/1‑93 (MQ=255)

                                                          tATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGaa                              >  2:2454691/1‑93 (MQ=255)

                                                          tATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGaa                              <  2:4180623/93‑1 (MQ=255)

                                                             ttGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAAt                           <  1:4322602/93‑1 (MQ=255)

                                                             ttGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAAt                           <  1:1131280/93‑1 (MQ=255)

                                                             ttGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAAt                           <  1:4401433/93‑1 (MQ=255)

                                                              tGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATa                          <  1:4295650/93‑1 (MQ=255)

                                                              tGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATa                          <  2:1652069/93‑1 (MQ=255)

                                                                ccGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAgg                        <  2:4091957/93‑1 (MQ=255)

                                                                ccGATCCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAgg                        <  2:4091959/93‑1 (MQ=255)

                                                                  gATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGgcg                      <  2:3752255/93‑1 (MQ=255)

                                                                   aTGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGgcgc                     >  2:1955565/1‑93 (MQ=255)

                                                                   aTGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGgcgc                     <  2:588121/93‑1 (MQ=255)

                                                                   aTGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGgcgc                     >  2:3410511/1‑93 (MQ=255)

                                                                        ggTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTg                >  1:2130967/1‑93 (MQ=255)

                                                                        ggTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTg                >  1:3765322/1‑93 (MQ=255)

                                                                          ttAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGtt              >  1:1524437/1‑93 (MQ=255)

                                                                            aaCTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAg            <  1:3221424/93‑1 (MQ=255)

                                                                            aaCTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAg            <  2:3946410/93‑1 (MQ=255)

                                                                             aCTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGc           <  2:1411627/93‑1 (MQ=255)

                                                                             aCTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGc           <  2:4231718/93‑1 (MQ=255)

                                                                              cTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCa          <  1:3306265/93‑1 (MQ=255)

                                                                              cTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCa          <  1:4387525/93‑1 (MQ=255)

                                                                              cTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCa          <  1:4160494/93‑1 (MQ=255)

                                                                               tCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCAt         >  2:1542103/1‑93 (MQ=255)

                                                                               tCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCAt         >  2:730749/1‑93 (MQ=255)

                                                                                 cGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGa       >  1:378112/1‑93 (MQ=255)

                                                                                  ggCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGaa      <  2:3077555/93‑1 (MQ=255)

                                                                                  ggCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGaa      <  1:420021/93‑1 (MQ=255)

                                                                                  ggCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGaa      <  2:893648/93‑1 (MQ=255)

                                                                                    ggAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACa    <  1:877170/92‑1 (MQ=255)

                                                                                    cGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACa    <  1:515343/93‑1 (MQ=255)

                                                                                     gAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACAt   <  1:3738564/93‑1 (MQ=255)

GGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTAGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATT  >  NC_000913/2123652‑2123830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTAGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCA  >  NC_000913/2123650‑2123834

GCGGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGT                                                                                             <  SRR4302535.2200439/94‑1 (MQ=60)

  GGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGGTGCCGGTTAACTCCGGCGAGTGGTTA                                                                                           >  SRR4302535.1288000/1‑94 (MQ=60)

  GGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTA                                                                                           >  SRR4302535.4260572/1‑94 (MQ=60)

    TAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAAT                                                                                         <  SRR4302535.912009/94‑1 (MQ=60)

    TAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAAT                                                                                         <  SRR4302535.1115869/94‑1 (MQ=60)

    TAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGCATTTGCCGATGCTGGCTAATTCCGGCGAGTGGTTAAT                                                                                         >  SRR4302535.4271178/1‑94 (MQ=60)

     AATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATG                                                                                        <  SRR4302535.4650400/94‑1 (MQ=60)

     AATGACCCGCACCTCATGACCTTGTACCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATG                                                                                        <  SRR4302535.802095/94‑1 (MQ=60)

       AGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCC                                                                                      <  SRR4302535.3942741/94‑1 (MQ=60)

         ACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGT                                                                                    >  SRR4302535.1278210/1‑94 (MQ=60)

           CCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAG                                                                                  <  SRR4302535.112900/94‑1 (MQ=60)

            CGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGA                                                                                 <  SRR4302535.1668594/94‑1 (MQ=60)

                  TCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTA                                                                           >  SRR4302535.2239146/1‑94 (MQ=60)

                   CATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTAT                                                                          <  SRR4302535.4429720/94‑1 (MQ=60)

                     TGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTT                                                                        <  SRR4302535.2680634/94‑1 (MQ=60)

                       ACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTC                                                                      >  SRR4302535.2495753/1‑94 (MQ=60)

                          TTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATA                                                                   <  SRR4302535.1080536/94‑1 (MQ=60)

                           TGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAA                                                                  <  SRR4302535.462815/94‑1 (MQ=60)

                            GTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAAT                                                                 <  SRR4302535.1659684/94‑1 (MQ=60)

                             TGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATC                                                                <  SRR4302535.279468/94‑1 (MQ=60)

                              GCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCC                                                               <  SRR4302535.1609336/94‑1 (MQ=60)

                              GCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCC                                                               <  SRR4302535.2942764/94‑1 (MQ=60)

                                 ctgagcgattcgcccgtcgCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGG                                                            <  SRR4302535.585480/75‑1 (MQ=60)

                                    AGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTAC                                                         >  SRR4302535.2378497/1‑94 (MQ=60)

                                    AGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTAC                                                         <  SRR4302535.3087217/94‑1 (MQ=60)

                                     GCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACT                                                        <  SRR4302535.4428199/94‑1 (MQ=60)

                                      CCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTC                                                       >  SRR4302535.80678/1‑94 (MQ=60)

                                       CATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCC                                                      >  SRR4302535.4424646/1‑94 (MQ=60)

                                       CATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCC                                                      <  SRR4302535.1024550/94‑1 (MQ=60)

                                       CATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCC                                                      >  SRR4302535.214218/1‑94 (MQ=60)

                                        ATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCG                                                     >  SRR4302535.3185425/1‑94 (MQ=60)

                                        ATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCG                                                     <  SRR4302535.426532/94‑1 (MQ=60)

                                        ATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCG                                                     >  SRR4302535.1186868/1‑94 (MQ=60)

                                          TCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGT                                                   <  SRR4302535.2979297/94‑1 (MQ=60)

                                            CACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTAC                                                 <  SRR4302535.4649486/94‑1 (MQ=60)

                                             ACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACG                                                >  SRR4302535.2143535/1‑94 (MQ=60)

                                             ACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACG                                                >  SRR4302535.4386975/1‑94 (MQ=60)

                                             ACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACG                                                >  SRR4302535.1788612/1‑94 (MQ=60)

                                              CCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGC                                               >  SRR4302535.2878550/1‑94 (MQ=60)

                                              CCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGC                                               >  SRR4302535.2678846/1‑94 (MQ=60)

                                               CATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGCCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGCACTCCGGTACGCT                                              >  SRR4302535.806181/1‑94 (MQ=60)

                                                ATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTT                                             <  SRR4302535.1674687/94‑1 (MQ=60)

                                                  CTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCT                                           <  SRR4302535.2976582/94‑1 (MQ=60)

                                                  CTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCT                                           <  SRR4302535.3535782/94‑1 (MQ=60)

                                                  CTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCT                                           >  SRR4302535.782303/1‑94 (MQ=60)

                                                   TCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTC                                          <  SRR4302535.36181/94‑1 (MQ=60)

                                                    CGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCA                                         <  SRR4302535.736554/94‑1 (MQ=60)

                                                    CGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCA                                         <  SRR4302535.4639820/94‑1 (MQ=60)

                                                     GCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAG                                        >  SRR4302535.1294783/1‑94 (MQ=60)

                                                     GCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAG                                        <  SRR4302535.3219453/94‑1 (MQ=60)

                                                     GCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAG                                        <  SRR4302535.1570565/94‑1 (MQ=60)

                                                         GGGTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAG                                    >  SRR4302535.3114038/1‑94 (MQ=60)

                                                           GTATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAA                                  >  SRR4302535.4539030/1‑94 (MQ=60)

                                                            TATTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAA                                 <  SRR4302535.4579390/94‑1 (MQ=60)

                                                              TTTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAAT                               <  SRR4302535.4489940/94‑1 (MQ=60)

                                                               TTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATA                              <  SRR4302535.4352173/94‑1 (MQ=60)

                                                               TTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATA                              <  SRR4302535.1139355/94‑1 (MQ=60)

                                                               TTGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATA                              <  SRR4302535.4431552/94‑1 (MQ=60)

                                                                TGCCGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAG                             <  SRR4302535.4325043/94‑1 (MQ=60)

                                                                   CGATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCG                          >  SRR4302535.3513319/1‑94 (MQ=60)

                                                                     ATGCCGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCG                        <  SRR4302535.3735664/94‑1 (MQ=60)

                                                                         CGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTG                    >  SRR4302535.3791328/1‑94 (MQ=60)

                                                                         CGGTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTG                    >  SRR4302535.2146331/1‑94 (MQ=60)

                                                                           GTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTT                  <  SRR4302535.2563134/94‑1 (MQ=60)

                                                                           GTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTT                  >  SRR4302535.1535422/1‑94 (MQ=60)

                                                                           GTTAACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTT                  <  SRR4302535.485138/94‑1 (MQ=60)

                                                                              AACTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGC               <  SRR4302535.3243747/94‑1 (MQ=60)

                                                                                CTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCAT             <  SRR4302535.4188969/94‑1 (MQ=60)

                                                                                CTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCAT             <  SRR4302535.4417538/94‑1 (MQ=60)

                                                                                CTCCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCAT             <  SRR4302535.3329272/94‑1 (MQ=60)

                                                                                  CCGGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGA           >  SRR4302535.380732/1‑94 (MQ=60)

                                                                                    GGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAAC         <  SRR4302535.422957/94‑1 (MQ=60)

                                                                                    GGCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAAC         <  SRR4302535.3805835/94‑1 (MQ=60)

                                                                                     GCGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACA        <  SRR4302535.2095024/94‑1 (MQ=60)

                                                                                      GGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACAT       <  SRR4302535.883381/94‑1 (MQ=60)

                                                                                      CGAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACAT       <  SRR4302535.518944/94‑1 (MQ=60)

                                                                                       GAGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATT      <  SRR4302535.3764440/94‑1 (MQ=60)

                                                                                        AGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTA     <  SRR4302535.4195133/94‑1 (MQ=60)

                                                                                        AGTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTA     >  SRR4302535.491096/1‑94 (MQ=60)

                                                                                         GTGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTAT    >  SRR4302535.481635/1‑94 (MQ=60)

                                                                                          TGGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATC   >  SRR4302535.1715429/1‑94 (MQ=60)

                                                                                           GGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCA  >  SRR4302535.1654848/1‑94 (MQ=60)

GCGGTAATGACCCGCACCTCATGACCTTGTGCCGCCAGCCATTCCACCATCTCGCCGGTGTATTTGCCGATGCCGGTTAACTCCGGCGAGTAGTTAATGCCGTAGACCAGTATTTTCATAATCCGGGTACTCCGGTACGCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCA  >  NC_000913/2123650‑2123834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`