Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,123,880 T→C Q123R (CAG→CGG)  wcaH ← GDP‑mannose mannosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,123,8800TC99.5% 670.3 / ‑4.8 214Q123R (CAG→CGG) wcaHGDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (79/134);  total (79/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01

Only 100 of 223 total aligned reads displayed.

TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG  >  NC_000913/2123790‑2123969
                                                                                          |                                                                                         
ttCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCAtgccgc                                                                                         <  2:3152588/93‑1 (MQ=255)
 tctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATAGTCATgccgct                                                                                        >  2:2451227/1‑93 (MQ=255)
   tcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCATCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCa                                                                                      >  1:2530412/1‑93 (MQ=255)
   tcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCa                                                                                      >  1:7700850/1‑93 (MQ=255)
   tcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCa                                                                                      <  2:2337789/93‑1 (MQ=255)
     aGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATc                                                                                    <  1:4002383/93‑1 (MQ=255)
      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                   <  2:5152644/93‑1 (MQ=255)
       cGAGAAAATAGGCGCGGTTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCg                                                                                  <  1:2085286/93‑1 (MQ=255)
       cGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCg                                                                                  <  1:4261329/93‑1 (MQ=255)
         agaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGc                                                                                >  1:803288/1‑93 (MQ=255)
             aaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTa                                                                            >  1:2499955/1‑93 (MQ=255)
                 ggCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAg                                                                        >  1:5077287/1‑93 (MQ=255)
                  gcgcGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGc                                                                       <  2:1893184/93‑1 (MQ=255)
                  gcgcGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGc                                                                       >  1:2347838/1‑93 (MQ=255)
                     cgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctct                                                                    >  2:4887552/1‑93 (MQ=255)
                      ggCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtctt                                                                   <  2:500904/93‑1 (MQ=255)
                      ggCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtctt                                                                   <  1:1331502/93‑1 (MQ=255)
                       gCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                  <  2:1340858/93‑1 (MQ=255)
                       gCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                  <  1:4842524/93‑1 (MQ=255)
                       gCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                  <  2:4175389/93‑1 (MQ=255)
                        cTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttct                                                                 >  1:6827981/1‑93 (MQ=255)
                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                                <  1:3217097/93‑1 (MQ=255)
                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                                >  2:253326/1‑93 (MQ=255)
                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                                >  2:7163222/1‑93 (MQ=255)
                          gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCttcttc                                                               <  2:7578736/93‑1 (MQ=255)
                           ttAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                              <  2:7219132/93‑1 (MQ=255)
                           ttAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                              <  1:7402537/93‑1 (MQ=255)
                           ttAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                              >  2:5366903/1‑93 (MQ=255)
                           ttAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                              <  1:2966437/93‑1 (MQ=255)
                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                            <  1:423040/93‑1 (MQ=255)
                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                            <  2:2223236/93‑1 (MQ=255)
                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                            <  2:1872138/93‑1 (MQ=255)
                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                            <  2:7324887/93‑1 (MQ=255)
                               cATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATa                                                          <  2:4818538/93‑1 (MQ=255)
                                aTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAc                                                         <  1:101558/93‑1 (MQ=255)
                                aTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAc                                                         >  1:5952928/1‑93 (MQ=255)
                                 tGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAct                                                        >  1:1878426/1‑93 (MQ=255)
                                 tGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAct                                                        >  1:3139742/1‑93 (MQ=255)
                                 tGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAct                                                        <  1:5005163/93‑1 (MQ=255)
                                  gAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActc                                                       >  1:6494860/1‑93 (MQ=255)
                                   aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActct                                                      <  1:5121877/93‑1 (MQ=255)
                                   aaCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActct                                                      >  1:1549574/1‑93 (MQ=255)
                                     cATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGa                                                    <  1:7802913/93‑1 (MQ=255)
                                     cATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGa                                                    <  1:2870712/93‑1 (MQ=255)
                                      aTTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGaa                                                   <  2:1414229/93‑1 (MQ=255)
                                      aTTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGaa                                                   >  1:3076877/1‑93 (MQ=255)
                                        tATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGc                                                 <  1:175258/93‑1 (MQ=255)
                                         ttCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCg                                                <  2:2233490/92‑1 (MQ=255)
                                            ccTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                             <  1:3902776/92‑1 (MQ=255)
                                             cgggCGAGCACCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                            <  2:1566543/91‑1 (MQ=255)
                                             cTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                            <  1:3437943/93‑1 (MQ=255)
                                                gCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCg                                         >  1:3869442/1‑93 (MQ=255)
                                                 cGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGa                                        <  2:7666003/93‑1 (MQ=255)
                                                   agcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                      >  1:6191051/1‑93 (MQ=255)
                                                    gcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca                                     <  1:3358413/93‑1 (MQ=255)
                                                     cagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcac                                    <  1:6022621/93‑1 (MQ=255)
                                                      agcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacc                                   <  2:3389124/93‑1 (MQ=255)
                                                           gTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATa                              <  1:5482245/93‑1 (MQ=255)
                                                           gTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATa                              >  1:1849943/1‑93 (MQ=255)
                                                            tCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACCGCTCTTTTTCCGATACTCTGAACCAACAACAGAGCCCAACATag                             >  1:3727201/1‑93 (MQ=255)
                                                            tCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATag                             <  2:7006130/93‑1 (MQ=255)
                                                            tCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATag                             >  2:3959362/1‑93 (MQ=255)
                                                              cGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtg                           <  2:601328/93‑1 (MQ=255)
                                                              cGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtg                           <  2:7725099/93‑1 (MQ=255)
                                                                 cGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtgagt                        <  2:711557/93‑1 (MQ=255)
                                                                 cGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtgagg                        >  1:4476095/1‑92 (MQ=255)
                                                                  gTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtgagtg                       <  1:6605256/93‑1 (MQ=255)
                                                                  gTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtgagtg                       >  1:6421169/1‑93 (MQ=255)
                                                                  ctCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtgagtg                       <  1:2589517/92‑1 (MQ=255)
                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                      >  2:2967188/1‑93 (MQ=255)
                                                                     agccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggtg                    <  2:822094/93‑1 (MQ=255)
                                                                     agccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggtg                    <  2:1701048/93‑1 (MQ=255)
                                                                      gccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGa                   >  1:1462223/1‑93 (MQ=255)
                                                                      gccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGa                   >  2:1217691/1‑93 (MQ=255)
                                                                      gccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGa                   >  2:3874359/1‑93 (MQ=255)
                                                                       ccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaa                  >  2:4223461/1‑93 (MQ=255)
                                                                        cagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaaa                 <  2:4571131/93‑1 (MQ=255)
                                                                         agcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAAt                <  1:4528416/93‑1 (MQ=255)
                                                                         agcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAAt                >  1:7061999/1‑93 (MQ=255)
                                                                          gcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATc               <  1:1829376/93‑1 (MQ=255)
                                                                          gcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATc               <  2:6169839/93‑1 (MQ=255)
                                                                          gcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATc               <  2:5529222/93‑1 (MQ=255)
                                                                           cgttAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATcc              <  2:636956/90‑1 (MQ=255)
                                                                            ggTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCg             >  1:949615/1‑93 (MQ=255)
                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt            >  1:6285007/1‑93 (MQ=255)
                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt            <  1:5546807/93‑1 (MQ=255)
                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt            <  2:2998851/93‑1 (MQ=255)
                                                                               aaTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGc          <  1:5638370/93‑1 (MQ=255)
                                                                                aTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGcc         <  1:3567948/93‑1 (MQ=255)
                                                                                aTCGTCATGCCGATCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTAcc         <  2:7023641/93‑1 (MQ=255)
                                                                                 tCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCa        <  1:1100356/93‑1 (MQ=255)
                                                                                  cGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCag       <  1:3804162/93‑1 (MQ=255)
                                                                                   gTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCaga      <  2:3628969/93‑1 (MQ=255)
                                                                                   gTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCaga      >  2:3706314/1‑93 (MQ=255)
                                                                                    tCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCagag     <  2:3795477/93‑1 (MQ=255)
                                                                                     cATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAgaga    >  2:6871440/1‑93 (MQ=255)
                                                                                     cATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAgaga    <  2:4093946/93‑1 (MQ=255)
                                                                                      aTGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGaa   <  1:7618315/93‑1 (MQ=255)
                                                                                      aTGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGaa   <  2:1951455/93‑1 (MQ=255)
                                                                                       tgccgcTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAg  >  2:6668195/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG  >  NC_000913/2123790‑2123969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  NC_000913/2123788‑2123973
                                                                                            |                                                                                             
GCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCG                                                                                              <  SRR4302543.1879076/94‑1 (MQ=60)
  TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCT                                                                                            >  SRR4302543.7047024/1‑94 (MQ=60)
    CTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCATCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCA                                                                                          >  SRR4302543.2553439/1‑94 (MQ=60)
    CTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCA                                                                                          >  SRR4302543.7773730/1‑94 (MQ=60)
     TCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCAT                                                                                         <  SRR4302543.3036287/94‑1 (MQ=60)
     TCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCAT                                                                                         <  SRR4302543.2904591/94‑1 (MQ=60)
       AGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       <  SRR4302543.312678/94‑1 (MQ=60)
       AGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       <  SRR4302543.4690656/94‑1 (MQ=60)
       AGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       <  SRR4302543.4038087/94‑1 (MQ=60)
        GCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCG                                                                                      <  SRR4302543.3676438/94‑1 (MQ=60)
         CGAGAAAATAGGCGCGGTTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGG                                                                                     <  SRR4302543.2103905/94‑1 (MQ=60)
         CGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGG                                                                                     <  SRR4302543.4300034/94‑1 (MQ=60)
          GAGAAAATAGGCGTGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGC                                                                                    <  SRR4302543.547930/94‑1 (MQ=60)
          GAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGC                                                                                    >  SRR4302543.810355/1‑94 (MQ=60)
            GAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAG                                                                                  >  SRR4302543.2858976/1‑94 (MQ=60)
             AAAATAGGCGCGGCTTTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGT                                                                                 <  SRR4302543.1493647/94‑1 (MQ=60)
              AAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTA                                                                                >  SRR4302543.2522642/1‑94 (MQ=60)
                ATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAC                                                                              <  SRR4302543.1354144/94‑1 (MQ=60)
                  AGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAG                                                                            >  SRR4302543.5123347/1‑94 (MQ=60)
                   GGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGC                                                                           >  SRR4302543.2369125/1‑94 (MQ=60)
                    GCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCT                                                                          <  SRR4302543.1949267/94‑1 (MQ=60)
                      GCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCT                                                                        <  SRR4302543.3817156/94‑1 (MQ=60)
                      GCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCT                                                                        <  SRR4302543.7868444/94‑1 (MQ=60)
                       CGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTT                                                                       <  SRR4302543.6683839/94‑1 (MQ=60)
                        GGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTC                                                                      <  SRR4302543.1343371/94‑1 (MQ=60)
                        GGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTC                                                                      >  SRR4302543.2244678/1‑94 (MQ=60)
                         GCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCT                                                                     <  SRR4302543.4886679/94‑1 (MQ=60)
                         GCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCT                                                                     >  SRR4302543.6892441/1‑94 (MQ=60)
                           TGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTC                                                                   <  SRR4302543.3246162/94‑1 (MQ=60)
                             TTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCGTCCGGCAGTAACAGCTCTTCTTCCG                                                                 <  SRR4302543.6651565/94‑1 (MQ=60)
                             TTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCG                                                                 <  SRR4302543.7473593/94‑1 (MQ=60)
                             TTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCG                                                                 <  SRR4302543.5003417/94‑1 (MQ=60)
                             TTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCG                                                                 <  SRR4302543.2993375/94‑1 (MQ=60)
                               AGCATGAACATTATCACTGGCGAGCAGCGCGTCTGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAT                                                               >  SRR4302543.7540922/1‑94 (MQ=60)
                               AGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAT                                                               <  SRR4302543.426996/94‑1 (MQ=60)
                               AGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAT                                                               <  SRR4302543.6151823/94‑1 (MQ=60)
                                GCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATA                                                              >  SRR4302543.1666865/1‑94 (MQ=60)
                                 CATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAC                                                             <  SRR4302543.3197366/94‑1 (MQ=60)
                                 CATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAC                                                             >  SRR4302543.6007407/1‑94 (MQ=60)
                                  ATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACT                                                            >  SRR4302543.3167982/1‑94 (MQ=60)
                                  ATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACT                                                            <  SRR4302543.102497/94‑1 (MQ=60)
                                  ATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACT                                                            >  SRR4302543.1895303/1‑94 (MQ=60)
                                  ATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACT                                                            <  SRR4302543.1729158/94‑1 (MQ=60)
                                   TGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTC                                                           <  SRR4302543.5050720/94‑1 (MQ=60)
                                   TGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTC                                                           >  SRR4302543.6556048/1‑94 (MQ=60)
                                    GAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCT                                                          >  SRR4302543.1563431/1‑94 (MQ=60)
                                     AACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTG                                                         <  SRR4302543.5168186/94‑1 (MQ=60)
                                       CATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAA                                                       <  SRR4302543.2896861/94‑1 (MQ=60)
                                       CATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAA                                                       >  SRR4302543.3104549/1‑94 (MQ=60)
                                       CATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAA                                                       <  SRR4302543.7876467/94‑1 (MQ=60)
                                        ATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAG                                                      <  SRR4302543.3928335/94‑1 (MQ=60)
                                        ATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAG                                                      <  SRR4302543.400002/94‑1 (MQ=60)
                                        ATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAG                                                      <  SRR4302543.355009/94‑1 (MQ=60)
                                         TTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGC                                                     >  SRR4302543.4311174/1‑94 (MQ=60)
                                          TATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCG                                                    <  SRR4302543.176759/94‑1 (MQ=60)
                                           ATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGA                                                   <  SRR4302543.6520452/94‑1 (MQ=60)
                                              CCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAA                                                <  SRR4302543.3937238/94‑1 (MQ=60)
                                               CTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAC                                               <  SRR4302543.3468716/94‑1 (MQ=60)
                                               CTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAC                                               >  SRR4302543.5110831/1‑94 (MQ=60)
                                               CTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAC                                               <  SRR4302543.7535784/94‑1 (MQ=60)
                                                TGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACC                                              <  SRR4302543.1318398/94‑1 (MQ=60)
                                                 GGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCG                                             >  SRR4302543.3903611/1‑94 (MQ=60)
                                                  GCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGA                                            <  SRR4302543.4285818/94‑1 (MQ=60)
                                                    GAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGC                                          >  SRR4302543.6249550/1‑94 (MQ=60)
                                                      GCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCAC                                        <  SRR4302543.3388624/94‑1 (MQ=60)
                                                       CAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACC                                       <  SRR4302543.6078562/94‑1 (MQ=60)
                                                       CAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACC                                       <  SRR4302543.204693/94‑1 (MQ=60)
                                                        AGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCA                                      >  SRR4302543.3853443/1‑94 (MQ=60)
                                                         GCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCAC                                     <  SRR4302543.252183/94‑1 (MQ=60)
                                                            CGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATA                                  >  SRR4302543.1866584/1‑94 (MQ=60)
                                                            CGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATA                                  >  SRR4302543.1866583/1‑94 (MQ=60)
                                                             GTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACCGCTCTTTTTCCGATACTCTGAAccaacaacagagcccaacatag                                 >  SRR4302543.3759974/1‑72 (MQ=60)
                                                             GTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAG                                 >  SRR4302543.6078725/1‑94 (MQ=60)
                                                             GTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAG                                 <  SRR4302543.5531939/94‑1 (MQ=60)
                                                             GTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAG                                 >  SRR4302543.1858999/1‑94 (MQ=60)
                                                             GTCCGACGTCAGCCAGCGGTAATCGTCATGCCCCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAG                                 <  SRR4302543.5531945/94‑1 (MQ=60)
                                                               CCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTG                               >  SRR4302543.3279544/1‑94 (MQ=60)
                                                               CCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTG                               <  SRR4302543.5599750/94‑1 (MQ=60)
                                                               CCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTG                               <  SRR4302543.3673430/94‑1 (MQ=60)
                                                                CGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGA                              <  SRR4302543.5124671/94‑1 (MQ=60)
                                                                 GACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAG                             <  SRR4302543.6437416/94‑1 (MQ=60)
                                                                  ACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGT                            <  SRR4302543.2434570/94‑1 (MQ=60)
                                                                  ACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGT                            <  SRR4302543.4167415/94‑1 (MQ=60)
                                                                  ACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGG                            >  SRR4302543.4516723/1‑94 (MQ=60)
                                                                   CGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTG                           <  SRR4302543.4758931/94‑1 (MQ=60)
                                                                   CGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTG                           >  SRR4302543.6481673/1‑94 (MQ=60)
                                                                    GTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGG                          <  SRR4302543.6667509/94‑1 (MQ=60)
                                                                    GTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGG                          >  SRR4302543.4183666/1‑94 (MQ=60)
                                                                    CTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGG                          <  SRR4302543.2613059/94‑1 (MQ=60)
                                                                       AGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGA                       >  SRR4302543.1475408/1‑94 (MQ=60)
                                                                       AGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGA                       >  SRR4302543.6566212/1‑94 (MQ=60)
                                                                          CAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAAT                    >  SRR4302543.7130546/1‑94 (MQ=60)
                                                                           AGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATGCTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATC                   <  SRR4302543.4569631/94‑1 (MQ=60)
                                                                            GCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCC                  <  SRR4302543.1845806/94‑1 (MQ=60)
                                                                            GCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCC                  <  SRR4302543.1845807/94‑1 (MQ=60)
                                                                            GCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCC                  <  SRR4302543.6062655/94‑1 (MQ=60)
                                                                            GCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCC                  <  SRR4302543.7692412/94‑1 (MQ=60)
                                                                             CGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCG                 >  SRR4302543.957909/1‑94 (MQ=60)
                                                                              GGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGT                <  SRR4302543.6278074/94‑1 (MQ=60)
                                                                              GGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGT                <  SRR4302543.2639926/94‑1 (MQ=60)
                                                                              GGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGT                >  SRR4302543.6344214/1‑94 (MQ=60)
                                                                               GTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTG               <  SRR4302543.5597245/94‑1 (MQ=60)
                                                                                TAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGC              >  SRR4302543.5845/1‑94 (MQ=60)
                                                                                GAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGC              <  SRR4302543.3234049/94‑1 (MQ=60)
                                                                                 AATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCC             <  SRR4302543.5689767/94‑1 (MQ=60)
                                                                                  ATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCA            <  SRR4302543.7280789/94‑1 (MQ=60)
                                                                                  ATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCA            <  SRR4302543.3599502/94‑1 (MQ=60)
                                                                                   TCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAG           <  SRR4302543.1075526/94‑1 (MQ=60)
                                                                                   TCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAG           <  SRR4302543.1110267/94‑1 (MQ=60)
                                                                                   TCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAG           <  SRR4302543.2605181/94‑1 (MQ=60)
                                                                                    CGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGA          <  SRR4302543.3837685/94‑1 (MQ=60)
                                                                                     GTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAG         >  SRR4302543.3460396/1‑94 (MQ=60)
                                                                                     GTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAG         <  SRR4302543.7902269/94‑1 (MQ=60)
                                                                                       CATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAA       >  SRR4302543.956898/1‑94 (MQ=60)
                                                                                       CATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAA       <  SRR4302543.5470921/94‑1 (MQ=60)
                                                                                       CAGGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAA       <  SRR4302543.6926889/94‑1 (MQ=60)
                                                                                        ATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG      <  SRR4302543.7690625/94‑1 (MQ=60)
                                                                                        ATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG      >  SRR4302543.4554125/1‑94 (MQ=60)
                                                                                           CCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTA   >  SRR4302543.2157656/1‑94 (MQ=60)
                                                                                           CCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTA   >  SRR4302543.3365538/1‑94 (MQ=60)
                                                                                            CGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  SRR4302543.5633899/1‑94 (MQ=60)
                                                                                            CGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  <  SRR4302543.2031345/94‑1 (MQ=60)
                                                                                            CGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  <  SRR4302543.3144114/94‑1 (MQ=60)
                                                                                            CGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  <  SRR4302543.4979452/94‑1 (MQ=60)
                                                                                            |                                                                                             
GCTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  NC_000913/2123788‑2123973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑