Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,133,804	T→C	H106R (CAC→CGC)	wzb ←	putative protein‑tyrosine‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,133,804	0	T	C	99.6%	729.3 / NA	234	H106R (CAC→CGC)	wzb	putative protein‑tyrosine‑phosphatase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (115/118); minor base A (1/0); total (116/118)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893

gggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAc                                                                                        <  1:1430105/93‑1 (MQ=255)

     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   <  1:3540005/93‑1 (MQ=255)

     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   >  2:3373898/1‑93 (MQ=255)

     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   <  2:832698/93‑1 (MQ=255)

      aCCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCTCAGCGACCAAACa                                                                                  >  1:3927103/1‑93 (MQ=255)

      aCCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACa                                                                                  <  1:5090130/93‑1 (MQ=255)

          ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAt                                                                              >  1:6933901/1‑93 (MQ=255)

           tCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATc                                                                             <  2:1896985/93‑1 (MQ=255)

           tCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATc                                                                             >  1:3190862/1‑93 (MQ=255)

            cAAGTAATGTGTACGCCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCa                                                                            <  1:4925888/93‑1 (MQ=255)

             aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAc                                                                           <  2:5056985/93‑1 (MQ=255)

             aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAc                                                                           >  1:7174752/1‑93 (MQ=255)

               gTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         >  1:7127885/1‑93 (MQ=255)

               gTAATGGGTGCCCCTCTGCAAGCGTTTGCCGGCTTTTGCGATACGTATCGGGGATTTCACATTCGTTATCCAAGCGACCAAACTGCATCACtt                                                                         <  2:3570453/93‑1 (MQ=255)

                  aTGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGc                                                                      <  2:2636858/93‑1 (MQ=255)

                   tgtgTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGcc                                                                     >  1:3306577/1‑93 (MQ=255)

                   tgtgTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGcc                                                                     >  2:5947856/1‑93 (MQ=255)

                    gtgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcg                                                                    >  1:6384097/1‑93 (MQ=255)

                    gtgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTAT‑CCAGCGACCAAACAGCATCACTTTTCcgc                                                                   >  2:1036416/1‑93 (MQ=255)

                     tgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcgc                                                                   >  1:3836721/1‑93 (MQ=255)

                     tgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcgc                                                                   >  2:7687962/1‑93 (MQ=255)

                     tgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcgc                                                                   >  2:974923/1‑93 (MQ=255)

                        acacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCa                                                                <  1:431509/93‑1 (MQ=255)

                         cacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAt                                                               >  1:1322671/1‑93 (MQ=255)

                          acCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtc                                                              <  1:5420598/93‑1 (MQ=255)

                          acCGCTCCAAACTGTTCCCGGCTTTTTCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtc                                                              <  1:5731848/93‑1 (MQ=255)

                           ccGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtct                                                             >  1:2242094/1‑93 (MQ=255)

                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  2:1657875/93‑1 (MQ=255)

                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  2:7456822/93‑1 (MQ=255)

                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            >  1:1663625/1‑93 (MQ=255)

                            cGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtctc                                                            <  1:1913310/93‑1 (MQ=255)

                             gCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGGTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                           >  2:6584389/1‑93 (MQ=255)

                              cTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATCTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCg                                                          >  2:4822592/1‑93 (MQ=255)

                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        >  2:6142101/1‑93 (MQ=255)

                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  1:109208/93‑1 (MQ=255)

                                  aaaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGc                                                      >  2:6317637/1‑93 (MQ=255)

                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGGTTTCACATTCGTTATCCCAGAGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTccc                                                     >  2:6603996/1‑93 (MQ=255)

                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     >  2:883985/1‑93 (MQ=255)

                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGCTGcc                                                     >  2:3640909/1‑93 (MQ=255)

                                    aCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCa                                                    <  1:6010157/93‑1 (MQ=255)

                                    aCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCa                                                    <  1:7608300/93‑1 (MQ=255)

                                    aCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCa                                                    <  2:4996364/93‑1 (MQ=255)

                                     cGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAt                                                   >  1:455410/1‑93 (MQ=255)

                                     cGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAt                                                   <  1:4592395/93‑1 (MQ=255)

                                       tttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 <  2:2200586/93‑1 (MQ=255)

                                         tCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCg                                               >  1:4795097/1‑93 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             <  1:2457072/93‑1 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             >  2:3515744/1‑93 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             <  1:7431350/93‑1 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             >  2:706424/1‑93 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             >  2:3283848/1‑93 (MQ=255)

                                            cGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAt                                            >  2:1734732/1‑93 (MQ=255)

                                             ggCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATa                                           <  2:4631638/93‑1 (MQ=255)

                                              gCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATaa                                          >  2:1053533/1‑93 (MQ=255)

                                               gttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAg                                         <  1:6736394/92‑1 (MQ=255)

                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  2:4548375/1‑93 (MQ=255)

                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  1:7874492/1‑93 (MQ=255)

                                                 tttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCg                                       <  2:5561356/93‑1 (MQ=255)

                                                  ttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGt                                      <  2:5707204/93‑1 (MQ=255)

                                                  ttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGt                                      <  1:4195069/93‑1 (MQ=255)

                                                    gCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTc                                    >  2:2880262/1‑93 (MQ=255)

                                                      gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCGTAAGCGTTCGa                                  <  1:551592/93‑1 (MQ=255)

                                                      gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  >  1:4528635/1‑93 (MQ=255)

                                                      gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  >  1:2929606/1‑93 (MQ=255)

                                                      gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  <  2:6842917/93‑1 (MQ=255)

                                                       aTACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGat                                 <  1:6563929/93‑1 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGTTGCCATCTCGCATAAGCGTTCGata                                >  2:341534/1‑93 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATCAGCGTTCGtta                                >  2:1368144/1‑93 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  1:1046531/93‑1 (MQ=255)

                                                         aCGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGatat                               <  1:2730444/93‑1 (MQ=255)

                                                          cGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATg                              <  2:6813772/93‑1 (MQ=255)

                                                          cGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATg                              >  2:1298115/1‑93 (MQ=255)

                                                           ggATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATgg                             <  1:7709052/93‑1 (MQ=255)

                                                           ggATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATgg                             <  1:92405/93‑1 (MQ=255)

                                                            gATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgc                            <  2:7351979/93‑1 (MQ=255)

                                                             aTCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcg                           >  1:643557/1‑93 (MQ=255)

                                                              tCGGGGATTTCACATTCGTTGTCCCAGCTACCAAACAGAATCACTTTGCCGCGCATCTCCGCTGCCATCTCGCATAAGCGTTCGATATGgtgc                          >  2:981705/1‑93 (MQ=255)

                                                              tCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcgc                          >  1:6157648/1‑93 (MQ=255)

                                                                   gATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                     >  1:558956/1‑93 (MQ=255)

                                                                    aTTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTcc                    >  2:2706408/1‑93 (MQ=255)

                                                                    aTTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTcc                    <  1:6338506/93‑1 (MQ=255)

                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   >  2:5500883/1‑93 (MQ=255)

                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   >  2:1403481/1‑93 (MQ=255)

                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   >  1:261722/1‑93 (MQ=255)

                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   <  2:7634768/93‑1 (MQ=255)

                                                                      ttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAt                  <  1:2903261/93‑1 (MQ=255)

                                                                       tCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATg                 <  2:2006918/93‑1 (MQ=255)

                                                                        cacaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                >  2:7908263/1‑93 (MQ=255)

                                                                         acaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGt               >  1:1462588/1‑93 (MQ=255)

                                                                          caTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTc              >  2:5819262/1‑93 (MQ=255)

                                                                           aTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCa             >  2:227981/1‑93 (MQ=255)

                                                                           aTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCa             <  1:7338126/93‑1 (MQ=255)

                                                                            ttCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaa            >  2:4769255/1‑93 (MQ=255)

                                                                               gTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAt         >  1:4549227/1‑93 (MQ=255)

                                                                               gTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAt         >  2:250916/1‑93 (MQ=255)

                                                                               gTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAt         >  2:5301049/1‑93 (MQ=255)

                                                                                 tATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCAATGGTCAAAATCa       <  2:7282683/93‑1 (MQ=255)

                                                                                     ccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTc   <  1:5216223/93‑1 (MQ=255)

                                                                                     ccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTc   <  2:5919483/93‑1 (MQ=255)

                                                                                      cAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCg  >  2:2989091/1‑93 (MQ=255)

GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133711‑2133897

TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGC                                                                                               <  SRR4302543.2201978/94‑1 (MQ=60)

    GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACC                                                                                           >  SRR4302543.1185586/1‑94 (MQ=60)

    GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACC                                                                                           >  SRR4302543.964849/1‑94 (MQ=60)

    GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACC                                                                                           <  SRR4302543.1443039/94‑1 (MQ=60)

      GCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAA                                                                                         <  SRR4302543.4953174/94‑1 (MQ=60)

       CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAA                                                                                        >  SRR4302543.4451530/1‑94 (MQ=60)

        AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCATCGACCAAAC                                                                                       >  SRR4302543.5582835/1‑94 (MQ=60)

         GACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCTCAGCGACCAAACA                                                                                      >  SRR4302543.3961767/1‑94 (MQ=60)

         GACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACA                                                                                      <  SRR4302543.3571471/94‑1 (MQ=60)

          ACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAG                                                                                     <  SRR4302543.2431287/94‑1 (MQ=60)

          ACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAG                                                                                     <  SRR4302543.5136255/94‑1 (MQ=60)

             GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAT                                                                                  >  SRR4302543.6347277/1‑94 (MQ=60)

             GTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAT                                                                                  >  SRR4302543.6999126/1‑94 (MQ=60)

              TTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACATCATC                                                                                 >  SRR4302543.5566445/1‑94 (MQ=60)

              TTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATC                                                                                 >  SRR4302543.3219633/1‑94 (MQ=60)

                CAAGTAATGTGTACGCCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                               <  SRR4302543.4970760/94‑1 (MQ=60)

                CAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAC                                                                               >  SRR4302543.7244405/1‑94 (MQ=60)

                 AAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAACCACAATCACT                                                                              >  SRR4302543.6483842/1‑94 (MQ=60)

                  AGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTT                                                                             >  SRR4302543.7197144/1‑94 (MQ=60)

                  AGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGAATCACTT                                                                             >  SRR4302543.7814733/1‑94 (MQ=60)

                     AATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGC                                                                          >  SRR4302543.4670691/1‑94 (MQ=60)

                      ATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCC                                                                         >  SRR4302543.3336416/1‑94 (MQ=60)

                      ATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCC                                                                         >  SRR4302543.753750/1‑94 (MQ=60)

                       TGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCG                                                                        >  SRR4302543.6444182/1‑94 (MQ=60)

                       TGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCG                                                                        >  SRR4302543.6444180/1‑94 (MQ=60)

                        GTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGC                                                                       >  SRR4302543.3870583/1‑94 (MQ=60)

                            ACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAT                                                                   <  SRR4302543.435551/94‑1 (MQ=60)

                            ACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAT                                                                   >  SRR4302543.1334419/1‑94 (MQ=60)

                              ACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCT                                                                 <  SRR4302543.5469886/94‑1 (MQ=60)

                              ACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCT                                                                 >  SRR4302543.2262425/1‑94 (MQ=60)

                              ACCGCTCCAAACTGTTCCCGGCTTTTTCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCT                                                                 <  SRR4302543.5784150/94‑1 (MQ=60)

                               CCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTC                                                                >  SRR4302543.1678529/1‑94 (MQ=60)

                                CGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCC                                                               <  SRR4302543.6803323/94‑1 (MQ=60)

                                CGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCC                                                               <  SRR4302543.1930555/94‑1 (MQ=60)

                                   TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGT                                                            <  SRR4302543.5501202/94‑1 (MQ=60)

                                   TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGT                                                            <  SRR4302543.1081201/94‑1 (MQ=60)

                                   TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGT                                                            <  SRR4302543.1590790/94‑1 (MQ=60)

                                    GCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTG                                                           <  SRR4302543.110207/94‑1 (MQ=60)

                                      AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCC                                                         >  SRR4302543.1766995/1‑94 (MQ=60)

                                        ACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAT                                                       <  SRR4302543.6065577/94‑1 (MQ=60)

                                        ACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAT                                                       >  SRR4302543.459652/1‑94 (MQ=60)

                                        ACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAT                                                       <  SRR4302543.7680535/94‑1 (MQ=60)

                                         CGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATC                                                      <  SRR4302543.4634105/94‑1 (MQ=60)

                                         CGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATC                                                      >  SRR4302543.7201768/1‑94 (MQ=60)

                                            TTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCG                                                   >  SRR4302543.4838923/1‑94 (MQ=60)

                                            TTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCG                                                   <  SRR4302543.979645/94‑1 (MQ=60)

                                              CCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCA                                                 <  SRR4302543.4597475/94‑1 (MQ=60)

                                               CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                                <  SRR4302543.2479227/94‑1 (MQ=60)

                                               CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                                >  SRR4302543.6725487/1‑94 (MQ=60)

                                               CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                                <  SRR4302543.7502598/94‑1 (MQ=60)

                                                CGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATA                                               >  SRR4302543.3220703/1‑94 (MQ=60)

                                                 GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                              <  SRR4302543.4475998/94‑1 (MQ=60)

                                                 GGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAA                                              <  SRR4302543.1637145/94‑1 (MQ=60)

                                                  GCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAG                                             <  SRR4302543.7711674/94‑1 (MQ=60)

                                                   GTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            <  SRR4302543.6800025/94‑1 (MQ=60)

                                                   CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            <  SRR4302543.139274/94‑1 (MQ=60)

                                                   CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            >  SRR4302543.7948618/1‑94 (MQ=60)

                                                   CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            <  SRR4302543.7156959/94‑1 (MQ=60)

                                                      TTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTT                                         <  SRR4302543.4233106/94‑1 (MQ=60)

                                                       TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        <  SRR4302543.5139975/94‑1 (MQ=60)

                                                        GCGATACTGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCG                                       <  SRR4302543.856821/94‑1 (MQ=60)

                                                        GCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCG                                       <  SRR4302543.42783/94‑1 (MQ=60)

                                                         CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                      >  SRR4302543.2956214/1‑94 (MQ=60)

                                                         CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                      >  SRR4302543.4569852/1‑94 (MQ=60)

                                                          GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCGTAAGCGTTCGAT                                     <  SRR4302543.556666/94‑1 (MQ=60)

                                                          GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                     >  SRR4302543.3872393/1‑94 (MQ=60)

                                                          GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                     <  SRR4302543.7263496/94‑1 (MQ=60)

                                                          GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                     >  SRR4302543.2753773/1‑94 (MQ=60)

                                                          GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                     <  SRR4302543.6137432/94‑1 (MQ=60)

                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    >  SRR4302543.3970750/1‑94 (MQ=60)

                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    <  SRR4302543.6625695/94‑1 (MQ=60)

                                                           ATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATA                                    <  SRR4302543.5734358/94‑1 (MQ=60)

                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302543.1055902/94‑1 (MQ=60)

                                                            TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302543.4978598/94‑1 (MQ=60)

                                                             ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATG                                  <  SRR4302543.2755295/94‑1 (MQ=60)

                                                              CGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGG                                 <  SRR4302543.6402894/94‑1 (MQ=60)

                                                               GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                                <  SRR4302543.93270/94‑1 (MQ=60)

                                                               GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                                >  SRR4302543.2270702/1‑94 (MQ=60)

                                                               GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                                <  SRR4302543.7781980/94‑1 (MQ=60)

                                                                GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                               <  SRR4302543.4614781/94‑1 (MQ=60)

                                                                GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                               >  SRR4302543.649435/1‑94 (MQ=60)

                                                                GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                               <  SRR4302543.2790484/94‑1 (MQ=60)

                                                                 ATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGC                              >  SRR4302543.6215933/1‑94 (MQ=60)

                                                                    GGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTT                           <  SRR4302543.3771840/94‑1 (MQ=60)

                                                                      GGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTC                         >  SRR4302543.564081/1‑94 (MQ=60)

                                                                       GATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCC                        >  SRR4302543.3360542/1‑94 (MQ=60)

                                                                        ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCA                       <  SRR4302543.6398110/94‑1 (MQ=60)

                                                                        ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCA                       >  SRR4302543.264010/1‑94 (MQ=60)

                                                                        ATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCA                       <  SRR4302543.1475552/94‑1 (MQ=60)

                                                                          TTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATG                     <  SRR4302543.2929657/94‑1 (MQ=60)

                                                                            CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   >  SRR4302543.1475776/1‑94 (MQ=60)

                                                                             ACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTC                  >  SRR4302543.5306310/1‑94 (MQ=60)

                                                                             ACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTC                  <  SRR4302543.6519260/94‑1 (MQ=60)

                                                                             ACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTC                  >  SRR4302543.6113805/1‑94 (MQ=60)

                                                                             ACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTGTTCCATGGTC                  >  SRR4302543.3463913/1‑94 (MQ=60)

                                                                               ATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAA                <  SRR4302543.2208444/94‑1 (MQ=60)

                                                                               ATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAA                >  SRR4302543.2561821/1‑94 (MQ=60)

                                                                               ATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAA                <  SRR4302543.7413266/94‑1 (MQ=60)

                                                                               ATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAA                <  SRR4302543.7408719/94‑1 (MQ=60)

                                                                                TTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAA               >  SRR4302543.3149329/1‑94 (MQ=60)

                                                                                 TCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCACAA              >  SRR4302543.7706672/1‑94 (MQ=60)

                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             >  SRR4302543.4590658/1‑94 (MQ=60)

                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             >  SRR4302543.4590659/1‑94 (MQ=60)

                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             <  SRR4302543.2939032/94‑1 (MQ=60)

                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             >  SRR4302543.7138530/1‑94 (MQ=60)

                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             >  SRR4302543.2695066/1‑94 (MQ=60)

                                                                                  CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGACATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAGAAT             >  SRR4302543.4590660/1‑94 (MQ=60)

                                                                                     TATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTTGATATGGCGCTTTTCCATGGTCAAAATCAG          >  SRR4302543.538245/1‑94 (MQ=60)

                                                                                     TATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAG          >  SRR4302543.4968170/1‑94 (MQ=60)

                                                                                      ATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGG         <  SRR4302543.5896311/94‑1 (MQ=60)

                                                                                      ATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGG         >  SRR4302543.1377650/1‑94 (MQ=60)

                                                                                        CCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTC       <  SRR4302543.7477405/94‑1 (MQ=60)

                                                                                        CCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTC       <  SRR4302543.3115747/94‑1 (MQ=60)

                                                                                         CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG      <  SRR4302543.5263214/94‑1 (MQ=60)

                                                                                         CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAAATGGCGCTTTTCCATGGTCAAAATCAGGTCG      <  SRR4302543.6634305/94‑1 (MQ=60)

                                                                                          CAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGT     <  SRR4302543.4098615/94‑1 (MQ=60)

                                                                                           AGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCATGTCGTA    >  SRR4302543.5671281/1‑94 (MQ=60)

                                                                                           AGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTA    <  SRR4302543.7091180/94‑1 (MQ=60)

                                                                                            GCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAG   <  SRR4302543.4554628/94‑1 (MQ=60)

                                                                                             CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  SRR4302543.4600474/1‑94 (MQ=60)

TGGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133711‑2133897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`