Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,133,804	T→C	H106R (CAC→CGC)	wzb ←	putative protein‑tyrosine‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,133,804	0	T	C	99.4%	478.0 / ‑4.6	154	H106R (CAC→CGC)	wzb	putative protein‑tyrosine‑phosphatase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (73/80); total (74/80)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01

Only 100 of 158 total aligned reads displayed.

GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893

gggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAc                                                                                        >  1:2742078/1‑93 (MQ=255)

 ggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAcc                                                                                       >  1:1400093/1‑93 (MQ=255)

 ggCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAcc                                                                                       >  2:2660520/1‑93 (MQ=255)

    aGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCaaa                                                                                    >  2:4575305/1‑93 (MQ=255)

     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGGTTTCACATTCGTTATCCCAGCGAGCAAAc                                                                                   >  2:4102044/1‑93 (MQ=255)

     gACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAc                                                                                   <  1:1807031/93‑1 (MQ=255)

      aCCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACa                                                                                  <  1:2865591/93‑1 (MQ=255)

      aCCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACa                                                                                  <  2:2247967/93‑1 (MQ=255)

        cGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGc                                                                                >  2:2123166/1‑93 (MQ=255)

          ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAt                                                                              <  2:676770/93‑1 (MQ=255)

          ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAt                                                                              <  1:4277932/93‑1 (MQ=255)

          ttCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCAt                                                                              >  2:516359/1‑93 (MQ=255)

             aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAc                                                                           >  2:2939608/1‑93 (MQ=255)

             aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAc                                                                           >  2:1937290/1‑93 (MQ=255)

             aaGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCAc                                                                           <  2:1007127/93‑1 (MQ=255)

              aGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACt                                                                          <  2:2495254/93‑1 (MQ=255)

               gTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         <  2:1353189/93‑1 (MQ=255)

               atAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACtt                                                                         <  1:3525048/92‑1 (MQ=255)

                tAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACttt                                                                        >  2:2606842/1‑93 (MQ=255)

                tAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACttt                                                                        >  2:66152/1‑93 (MQ=255)

                tAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACttt                                                                        >  2:934462/1‑93 (MQ=255)

                tAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACttt                                                                        <  1:1166359/93‑1 (MQ=255)

                tAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACttt                                                                        <  1:1481327/93‑1 (MQ=255)

                   tgtgTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGcc                                                                     >  1:1305946/1‑93 (MQ=255)

                    gtgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcg                                                                    >  1:1636232/1‑93 (MQ=255)

                     tgtACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCcgc                                                                   <  1:1721148/93‑1 (MQ=255)

                       tACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCgcgc                                                                 <  2:4050128/93‑1 (MQ=255)

                        acacCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCa                                                                <  2:3778918/93‑1 (MQ=255)

                          cccGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtc                                                              <  1:2408806/92‑1 (MQ=255)

                          acCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtc                                                              <  1:1076160/93‑1 (MQ=255)

                          acCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtc                                                              <  2:3987477/93‑1 (MQ=255)

                           ccGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCAtct                                                             <  2:318594/93‑1 (MQ=255)

                             gCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                           <  2:1691943/93‑1 (MQ=255)

                             gCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                           <  2:179155/93‑1 (MQ=255)

                             gCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                           <  1:769765/93‑1 (MQ=255)

                             gCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTcc                                                           >  1:594632/1‑93 (MQ=255)

                               tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         <  1:4076228/93‑1 (MQ=255)

                               tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         >  2:3450874/1‑93 (MQ=255)

                               tGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCgg                                                         >  1:502997/1‑93 (MQ=255)

                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  2:2554475/93‑1 (MQ=255)

                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        >  2:3441490/1‑93 (MQ=255)

                                gCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGt                                                        <  2:53257/93‑1 (MQ=255)

                                   aaCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGcc                                                     <  1:2029235/93‑1 (MQ=255)

                                    aCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGGCa                                                    >  1:3294903/1‑93 (MQ=255)

                                    aCGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCa                                                    <  2:552687/93‑1 (MQ=255)

                                      gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATTCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  >  1:3734671/1‑93 (MQ=255)

                                      gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  <  2:3161698/93‑1 (MQ=255)

                                      gTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtc                                                  >  2:2891579/1‑93 (MQ=255)

                                       tttGCCGGGTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 <  1:375577/93‑1 (MQ=255)

                                       tttCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAtct                                                 >  2:3302334/1‑93 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCa                                             <  1:2237649/93‑1 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGACATCTCGCa                                             >  1:4207567/1‑93 (MQ=255)

                                           ccGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCCGTGCCATCTCGCa                                             >  2:2885797/1‑93 (MQ=255)

                                             ggCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATa                                           >  2:1735409/1‑93 (MQ=255)

                                              gCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATaa                                          >  2:1739264/1‑93 (MQ=255)

                                               cTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAg                                         <  2:38849/93‑1 (MQ=255)

                                               cTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAg                                         <  1:793021/93‑1 (MQ=255)

                                                ttttGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGc                                        >  2:2092783/1‑93 (MQ=255)

                                                   tGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGtt                                     <  2:3211466/93‑1 (MQ=255)

                                                   tGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGtt                                     <  2:2027324/93‑1 (MQ=255)

                                                   tGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGtt                                     <  1:35165/93‑1 (MQ=255)

                                                    gCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTc                                    >  1:124671/1‑93 (MQ=255)

                                                      gATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGa                                  <  1:111535/93‑1 (MQ=255)

                                                        tACTGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  2:1844425/93‑1 (MQ=255)

                                                        tACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGata                                <  1:3899527/93‑1 (MQ=255)

                                                         aCGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGatat                               >  1:611576/1‑93 (MQ=255)

                                                          cGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATg                              <  1:200348/93‑1 (MQ=255)

                                                          cGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGATGACATCTCGCATAAGCGTTCGATATg                              >  1:3058939/1‑93 (MQ=255)

                                                           ggATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATgg                             >  1:2942518/1‑93 (MQ=255)

                                                            gATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgc                            <  1:210760/93‑1 (MQ=255)

                                                             aTCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGgcg                           >  2:4266655/1‑93 (MQ=255)

                                                               cGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCTTCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCt                         <  1:1870007/93‑1 (MQ=255)

                                                               cGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCt                         >  1:4686432/1‑93 (MQ=255)

                                                                ggggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCtt                        >  1:87796/1‑93 (MQ=255)

                                                                ggggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCtt                        >  1:2709805/1‑93 (MQ=255)

                                                                 gggATTTCACATTGTTTATCCGAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCttt                       <  2:2699687/93‑1 (MQ=255)

                                                                 gggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCttt                       >  2:3891587/1‑93 (MQ=255)

                                                                 gggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCttt                       >  1:2072899/1‑93 (MQ=255)

                                                                 gggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCttt                       >  2:1675667/1‑93 (MQ=255)

                                                                  ggATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCtttt                      <  1:3254696/93‑1 (MQ=255)

                                                                   gATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTc                     >  1:3414685/1‑93 (MQ=255)

                                                                     tttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCa                   <  1:3676604/93‑1 (MQ=255)

                                                                      ttCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAt                  <  2:4441234/93‑1 (MQ=255)

                                                                        cacaTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATgg                <  2:3310800/93‑1 (MQ=255)

                                                                          caTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTc              >  2:3925114/1‑93 (MQ=255)

                                                                          caTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTc              >  2:1984332/1‑93 (MQ=255)

                                                                           aTTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCa             >  1:4165657/1‑93 (MQ=255)

                                                                            ttCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaa            >  2:1128684/1‑93 (MQ=255)

                                                                            ttCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaa            <  1:1985844/93‑1 (MQ=255)

                                                                              cGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaaa          <  2:3579970/93‑1 (MQ=255)

                                                                              cGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCaaaa          >  1:2292544/1‑93 (MQ=255)

                                                                                ttATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATc        <  1:696156/93‑1 (MQ=255)

                                                                                ttATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATc        >  2:1481941/1‑93 (MQ=255)

                                                                                  aTCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAg      <  1:1952184/93‑1 (MQ=255)

                                                                                   tCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAgg     >  1:3416961/1‑93 (MQ=255)

                                                                                   tCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAgg     >  2:4323813/1‑93 (MQ=255)

                                                                                    cccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGt    <  2:3060693/93‑1 (MQ=255)

                                                                                     ccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTc   >  2:2564344/1‑93 (MQ=255)

                                                                                     ccAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTc   >  1:241932/1‑93 (MQ=255)

                                                                                      cAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCg  <  2:2486075/93‑1 (MQ=255)

GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG  >  NC_000913/2133715‑2133893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133712‑2133897

GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCG                                                                                              <  SRR4302535.1815255/94‑1 (MQ=60)

  CGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGAC                                                                                            >  SRR4302535.2761621/1‑94 (MQ=60)

   GGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACC                                                                                           >  SRR4302535.1410089/1‑94 (MQ=60)

      CAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAA                                                                                        >  SRR4302535.4554222/1‑94 (MQ=60)

       AGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAAC                                                                                       >  SRR4302535.3665417/1‑94 (MQ=60)

        GACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACA                                                                                      <  SRR4302535.1820085/94‑1 (MQ=60)

         ACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAG                                                                                     <  SRR4302535.2885891/94‑1 (MQ=60)

           CGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCA                                                                                   >  SRR4302535.725857/1‑94 (MQ=60)

           CGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCA                                                                                   >  SRR4302535.178847/1‑94 (MQ=60)

             TTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATC                                                                                 <  SRR4302535.4307208/94‑1 (MQ=60)

              TCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCA                                                                                >  SRR4302535.1897133/1‑94 (MQ=60)

                AAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACT                                                                              <  SRR4302535.3146908/94‑1 (MQ=60)

                  ATAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTT                                                                            <  SRR4302535.3549527/94‑1 (MQ=60)

                   TAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTG                                                                           <  SRR4302535.1491919/94‑1 (MQ=60)

                   TAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTG                                                                           <  SRR4302535.282760/94‑1 (MQ=60)

                   TAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTG                                                                           <  SRR4302535.1174690/94‑1 (MQ=60)

                     ATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCC                                                                         >  SRR4302535.1315269/1‑94 (MQ=60)

                      TGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCG                                                                        >  SRR4302535.1648055/1‑94 (MQ=60)

                        TGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCG                                                                      <  SRR4302535.1733594/94‑1 (MQ=60)

                             CCCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCT                                                                 <  SRR4302535.2426149/94‑1 (MQ=60)

                             ACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCT                                                                 <  SRR4302535.1083810/94‑1 (MQ=60)

                               CGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCC                                                               >  SRR4302535.598800/1‑94 (MQ=60)

                                GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              <  SRR4302535.2118954/94‑1 (MQ=60)

                                GCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCG                                                              <  SRR4302535.775182/94‑1 (MQ=60)

                                 CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                             >  SRR4302535.1128159/1‑94 (MQ=60)

                                 CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                             >  SRR4302535.3420056/1‑94 (MQ=60)

                                 CTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGG                                                             >  SRR4302535.506514/1‑94 (MQ=60)

                                  TGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGT                                                            <  SRR4302535.4104117/94‑1 (MQ=60)

                                   GCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTG                                                           <  SRR4302535.2395268/94‑1 (MQ=60)

                                     AAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGGC                                                         >  SRR4302535.2716180/1‑94 (MQ=60)

                                      AACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGGCA                                                        >  SRR4302535.3317809/1‑94 (MQ=60)

                                      AACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCA                                                        <  SRR4302535.2043862/94‑1 (MQ=60)

                                       ACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCAT                                                       >  SRR4302535.4124613/1‑94 (MQ=60)

                                        CGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATTCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATC                                                      >  SRR4302535.3760518/1‑94 (MQ=60)

                                         GTTTCCCGGCTTTTACGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCT                                                     <  SRR4302535.433809/94‑1 (MQ=60)

                                          TTTGCCGGGTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTC                                                    <  SRR4302535.378179/94‑1 (MQ=60)

                                             CCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGACATCTCGCA                                                 >  SRR4302535.4236361/1‑94 (MQ=60)

                                              CCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCAT                                                <  SRR4302535.2253570/94‑1 (MQ=60)

                                                  CTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGC                                            <  SRR4302535.798594/94‑1 (MQ=60)

                                                      TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        >  SRR4302535.125547/1‑94 (MQ=60)

                                                      TGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTC                                        <  SRR4302535.35437/94‑1 (MQ=60)

                                                       GCGATACGGATCGGGGATCTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCG                                       <  SRR4302535.2016455/94‑1 (MQ=60)

                                                        CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                      <  SRR4302535.3445729/94‑1 (MQ=60)

                                                        CGATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGA                                      >  SRR4302535.1261158/1‑94 (MQ=60)

                                                         GATACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGAT                                     <  SRR4302535.112320/94‑1 (MQ=60)

                                                           TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   <  SRR4302535.3926240/94‑1 (MQ=60)

                                                           TACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATAT                                   >  SRR4302535.615834/1‑94 (MQ=60)

                                                            ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATG                                  >  SRR4302535.3080334/1‑94 (MQ=60)

                                                            ACGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGATGACATCTCGCATAAGCGTTCGATATG                                  >  SRR4302535.3080335/1‑94 (MQ=60)

                                                             CGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGG                                 >  SRR4302535.2963207/1‑94 (MQ=60)

                                                             CGGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGG                                 <  SRR4302535.201718/94‑1 (MQ=60)

                                                              GGATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGC                                <  SRR4302535.2273743/94‑1 (MQ=60)

                                                               GATCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCG                               <  SRR4302535.212210/94‑1 (MQ=60)

                                                                 TCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCT                             <  SRR4302535.1613730/94‑1 (MQ=60)

                                                                 TCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCT                             <  SRR4302535.693777/94‑1 (MQ=60)

                                                                 TCGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCT                             >  SRR4302535.4718203/1‑94 (MQ=60)

                                                                  CGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCTTCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTT                            <  SRR4302535.1883486/94‑1 (MQ=60)

                                                                  CGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTT                            >  SRR4302535.88413/1‑94 (MQ=60)

                                                                  CGGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTT                            >  SRR4302535.2729174/1‑94 (MQ=60)

                                                                   GGGGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTT                           >  SRR4302535.2087832/1‑94 (MQ=60)

                                                                     GGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTC                         >  SRR4302535.3438439/1‑94 (MQ=60)

                                                                     GGATTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTC                         <  SRR4302535.3277308/94‑1 (MQ=60)

                                                                        TTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAT                      <  SRR4302535.3702059/94‑1 (MQ=60)

                                                                        TTTCACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCAT                      <  SRR4302535.3702057/94‑1 (MQ=60)

                                                                           CACATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGT                   <  SRR4302535.507969/94‑1 (MQ=60)

                                                                             CATTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCA                 >  SRR4302535.4194181/1‑94 (MQ=60)

                                                                               TTCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAA               <  SRR4302535.2000145/94‑1 (MQ=60)

                                                                                TCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAA              >  SRR4302535.2308943/1‑94 (MQ=60)

                                                                                TCGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAA              >  SRR4302535.1435097/1‑94 (MQ=60)

                                                                                 CGTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAAT             >  SRR4302535.3898430/1‑94 (MQ=60)

                                                                                  GTTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATC            >  SRR4302535.2033967/1‑94 (MQ=60)

                                                                                   TTATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCA           <  SRR4302535.701037/94‑1 (MQ=60)

                                                                                     ATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGG         >  SRR4302535.3440731/1‑94 (MQ=60)

                                                                                     ATCCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGG         <  SRR4302535.1966233/94‑1 (MQ=60)

                                                                                       CCCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTC       >  SRR4302535.243591/1‑94 (MQ=60)

                                                                                        CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG      >  SRR4302535.419880/1‑94 (MQ=60)

                                                                                        CCAGCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCG      <  SRR4302535.844789/94‑1 (MQ=60)

                                                                                           GCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAG   <  SRR4302535.1816066/94‑1 (MQ=60)

                                                                                           GCGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAG   <  SRR4302535.1998386/94‑1 (MQ=60)

                                                                                            CGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  SRR4302535.904692/1‑94 (MQ=60)

GGCGGGCAGACCGTTCAAGTAATGTGTACACCGCTGCAAACGTTTCCCGGCTTTTGCGATACGGATCGGGGATTTCACATTCGTTATCCCAGTGACCAAACAGCATCACTTTGCCGCGCATCTCCGGTGCCATCTCGCATAAGCGTTCGATATGGCGCTTTTCCATGGTCAAAATCAGGTCGTAGT  >  NC_000913/2133712‑2133897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`