Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,146,005 T→C W104R (TGG→CGG)  b2069 → YegD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,146,0050TC97.3% 557.4 / 2.5 185W104R (TGG→CGG) b2069YegD
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (101/79);  total (104/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 192 total aligned reads displayed.

TAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGA  >  NC_000913/2145914‑2146095
                                                                                           |                                                                                          
     gcgAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTtg                                                                                      <  1:4543303/93‑1 (MQ=255)
       gaagaaGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTtgtg                                                                                    >  1:1589752/1‑93 (MQ=255)
        aagaagATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGa                                                                                   >  2:2215043/1‑93 (MQ=255)
        aagaagATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGa                                                                                   <  2:3350714/93‑1 (MQ=255)
         agaagaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGaa                                                                                  >  1:4624416/1‑93 (MQ=255)
         agaagaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGaa                                                                                  >  1:51101/1‑93 (MQ=255)
          gaagaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGaaa                                                                                 >  1:2282080/1‑93 (MQ=255)
          gaagaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGaaa                                                                                 >  2:1668660/1‑93 (MQ=255)
           aagaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAAt                                                                                <  2:2473750/93‑1 (MQ=255)
           aagaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAAt                                                                                <  2:3320523/93‑1 (MQ=255)
            agaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATc                                                                               >  2:2932147/1‑93 (MQ=255)
            agaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATc                                                                               >  1:4337505/1‑93 (MQ=255)
             gaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCa                                                                              >  2:2079694/1‑93 (MQ=255)
             gaTATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCa                                                                              >  2:648631/1‑93 (MQ=255)
              atatCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCAc                                                                             >  2:4607290/1‑93 (MQ=255)
               tatCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCAcc                                                                            >  2:2770883/1‑93 (MQ=255)
               tatCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCAcc                                                                            >  2:388709/1‑93 (MQ=255)
                 tCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCaa                                                                          >  2:3456234/1‑93 (MQ=255)
                   gATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCaaaa                                                                        >  2:503842/1‑93 (MQ=255)
                    aTGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAAt                                                                       <  2:4393327/93‑1 (MQ=255)
                    aTGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAAt                                                                       >  1:199342/1‑93 (MQ=255)
                    aTGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAAt                                                                       >  2:2832056/1‑93 (MQ=255)
                    aTGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAAt                                                                       <  2:4241359/93‑1 (MQ=255)
                    aTGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAAt                                                                       <  1:3881900/93‑1 (MQ=255)
                     tGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATc                                                                      <  2:3718206/93‑1 (MQ=255)
                     tGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATc                                                                      >  2:2420552/1‑93 (MQ=255)
                     tGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATc                                                                      <  1:388427/93‑1 (MQ=255)
                      gTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCg                                                                     >  1:2614354/1‑93 (MQ=255)
                       ttACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGt                                                                    <  2:1119076/93‑1 (MQ=255)
                          cGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTcc                                                                 >  2:4355718/1‑93 (MQ=255)
                           ggCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCt                                                                <  1:4218973/93‑1 (MQ=255)
                           cgCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTAGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCt                                                                <  1:1165734/92‑1 (MQ=255)
                             cGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCg                                                              <  1:477830/93‑1 (MQ=255)
                              gAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCgg                                                             >  1:2910289/1‑93 (MQ=255)
                              gAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCgg                                                             >  1:2904885/1‑93 (MQ=255)
                              gAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCgg                                                             >  2:441773/1‑93 (MQ=255)
                               aaaaGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGt                                                            >  2:4011681/1‑93 (MQ=255)
                               aaaaGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGt                                                            >  2:3634085/1‑93 (MQ=255)
                               aaaaGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGt                                                            <  1:4303198/93‑1 (MQ=255)
                                     gTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGc                                                      >  1:3100713/1‑93 (MQ=255)
                                      tGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCg                                                     >  1:1404002/1‑93 (MQ=255)
                                      tGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCg                                                     >  1:1242171/1‑93 (MQ=255)
                                         aGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCt                                                  <  2:2968609/93‑1 (MQ=255)
                                         aGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCt                                                  <  2:642259/93‑1 (MQ=255)
                                         aGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCt                                                  <  1:2800007/93‑1 (MQ=255)
                                           ttCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTa                                                >  2:2397201/1‑93 (MQ=255)
                                             cGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCTTTCCTCGGTGCCAGCGGCTTaaa                                              <  2:3096316/93‑1 (MQ=255)
                                             cGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTaaa                                              <  1:4182877/93‑1 (MQ=255)
                                              ggTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTaaaa                                             >  1:3643020/1‑93 (MQ=255)
                                               gTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAAc                                            >  1:2016753/1‑93 (MQ=255)
                                               gTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAAc                                            <  2:4151002/93‑1 (MQ=255)
                                               gTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAAc                                            <  1:4113951/93‑1 (MQ=255)
                                                tCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAAcc                                           <  2:1550778/93‑1 (MQ=255)
                                                  tttCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCgc                                         >  2:3602875/1‑93 (MQ=255)
                                                   ttCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTACAACCgca                                        >  2:513554/1‑93 (MQ=255)
                                                   ttCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCgca                                        >  1:2876694/1‑93 (MQ=255)
                                                   ttCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCgca                                        <  2:3274586/93‑1 (MQ=255)
                                                      cTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCgcagca                                     <  2:562199/93‑1 (MQ=255)
                                                      cTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCgcagca                                     >  1:926199/1‑93 (MQ=255)
                                                        cACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAgg                                   >  1:3695754/1‑93 (MQ=255)
                                                         aCTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGt                                  >  1:3743411/1‑93 (MQ=255)
                                                         aCTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGt                                  >  1:3235004/1‑93 (MQ=255)
                                                         aCTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGt                                  <  1:3180424/93‑1 (MQ=255)
                                                          cTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTa                                 <  2:231329/93‑1 (MQ=255)
                                                              cacaGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAgcgc                             <  1:73638/93‑1 (MQ=255)
                                                                caGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTg                           >  2:889835/1‑93 (MQ=255)
                                                                caGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTg                           >  1:337267/1‑93 (MQ=255)
                                                                caGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTg                           >  2:1613339/1‑93 (MQ=255)
                                                                 aGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGt                          <  2:2506934/93‑1 (MQ=255)
                                                                  gTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGtt                         >  2:1243245/1‑93 (MQ=255)
                                                                  gTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGtt                         <  2:4460003/93‑1 (MQ=255)
                                                                   tACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGttt                        >  2:1023605/1‑93 (MQ=255)
                                                                   tACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGttt                        >  1:3805565/1‑93 (MQ=255)
                                                                    aCATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTg                       >  1:4002704/1‑93 (MQ=255)
                                                                     cATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGa                      >  2:2426301/1‑93 (MQ=255)
                                                                     cATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGa                      >  2:2131856/1‑93 (MQ=255)
                                                                      aTTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAg                     <  1:338828/93‑1 (MQ=255)
                                                                      aTTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAg                     >  1:1524366/1‑93 (MQ=255)
                                                                       ttGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAgg                    >  1:4465994/1‑93 (MQ=255)
                                                                       ttGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAgg                    >  1:4140101/1‑93 (MQ=255)
                                                                       ttGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAgg                    >  1:2164460/1‑93 (MQ=255)
                                                                        tgatgaTCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGa                   <  2:744868/93‑1 (MQ=255)
                                                                         gatgatCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGAt                  >  1:2346189/1‑93 (MQ=255)
                                                                         gatgatCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGAt                  <  2:3781673/93‑1 (MQ=255)
                                                                               ccAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTc            <  1:223443/93‑1 (MQ=255)
                                                                               ccAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTc            <  1:114547/93‑1 (MQ=255)
                                                                                cAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCt           >  1:987628/1‑93 (MQ=255)
                                                                                 agaagaAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTg          >  1:1935659/1‑93 (MQ=255)
                                                                                  gaagaaGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTgc         >  2:4200975/1‑93 (MQ=255)
                                                                                  gaagaaGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTgc         >  1:1837564/1‑93 (MQ=255)
                                                                                  gaagaaGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTgc         >  2:2899787/1‑93 (MQ=255)
                                                                                    agaagTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTgcgc       >  1:249206/1‑93 (MQ=255)
                                                                                      aagTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCaa     <  1:2666653/93‑1 (MQ=255)
                                                                                       agTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAat    >  1:8222/1‑93 (MQ=255)
                                                                                       agTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAat    <  2:541534/93‑1 (MQ=255)
                                                                                        gtgcGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAatg   >  1:4019593/1‑93 (MQ=255)
                                                                                        gtgcGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAatg   >  1:630254/1‑93 (MQ=255)
                                                                                        gtgcGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAatg   >  1:1767253/1‑93 (MQ=255)
                                                                                        gtgcGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAatg   <  2:2426875/93‑1 (MQ=255)
                                                                                         tgtgGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAatga  <  2:2750154/93‑1 (MQ=255)
                                                                                           |                                                                                          
TAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGA  >  NC_000913/2145914‑2146095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGT  >  NC_000913/2145913‑2146098
                                                                                            |                                                                                             
ATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCCGAAGAAGTGCG                                                                                              >  SRR4302535.1719148/1‑94 (MQ=60)
ATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCG                                                                                              <  SRR4302535.3968198/94‑1 (MQ=60)
ATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCG                                                                                              >  SRR4302535.4310445/1‑94 (MQ=60)
   ACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTT                                                                                           <  SRR4302535.4405988/94‑1 (MQ=60)
      GCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGT                                                                                        <  SRR4302535.12272/94‑1 (MQ=60)
      GCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGT                                                                                        <  SRR4302535.4574376/94‑1 (MQ=60)
       CGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTG                                                                                       <  SRR4302535.2928770/94‑1 (MQ=60)
       CGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTG                                                                                       >  SRR4302535.1601226/1‑94 (MQ=60)
        GAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGA                                                                                      >  SRR4302535.90511/1‑94 (MQ=60)
        GAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGA                                                                                      <  SRR4302535.2005669/94‑1 (MQ=60)
         AAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAA                                                                                     >  SRR4302535.4655818/1‑94 (MQ=60)
         AAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAA                                                                                     >  SRR4302535.51479/1‑94 (MQ=60)
         AAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAA                                                                                     <  SRR4302535.3157253/94‑1 (MQ=60)
          AGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAA                                                                                    >  SRR4302535.2298382/1‑94 (MQ=60)
            AAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATC                                                                                  >  SRR4302535.4367168/1‑94 (MQ=60)
             AGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCA                                                                                 <  SRR4302535.2651111/94‑1 (MQ=60)
             AGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGGGCGGTTTGTGAAATCA                                                                                 >  SRR4302535.1773874/1‑94 (MQ=60)
               ATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACC                                                                               <  SRR4302535.1497394/94‑1 (MQ=60)
                   CGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAATTGCGGTTTGTGAAATCACCAAAA                                                                           >  SRR4302535.511419/1‑94 (MQ=60)
                    GATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAAT                                                                          >  SRR4302535.1558975/1‑94 (MQ=60)
                    GATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAAT                                                                          >  SRR4302535.182446/1‑94 (MQ=60)
                    GATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAAT                                                                          >  SRR4302535.200707/1‑94 (MQ=60)
                     ATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATC                                                                         <  SRR4302535.3908537/94‑1 (MQ=60)
                     ATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATC                                                                         >  SRR4302535.2195245/1‑94 (MQ=60)
                      TGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCG                                                                        >  SRR4302535.2633102/1‑94 (MQ=60)
                      TGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCG                                                                        <  SRR4302535.391126/94‑1 (MQ=60)
                        TTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTT                                                                      <  SRR4302535.2852164/94‑1 (MQ=60)
                        TTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTT                                                                      >  SRR4302535.4620080/1‑94 (MQ=60)
                            GGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTC                                                                  <  SRR4302535.4247841/94‑1 (MQ=60)
                            CGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTAGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTC                                                                  <  SRR4302535.1174059/94‑1 (MQ=60)
                             GCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCG                                                                 <  SRR4302535.4144757/94‑1 (MQ=60)
                              CGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGG                                                                <  SRR4302535.481181/94‑1 (MQ=60)
                              CGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGG                                                                >  SRR4302535.2925418/1‑94 (MQ=60)
                              CGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGG                                                                >  SRR4302535.2930848/1‑94 (MQ=60)
                                AAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTG                                                              <  SRR4302535.4332636/94‑1 (MQ=60)
                                     CGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGC                                                         <  SRR4302535.2631973/94‑1 (MQ=60)
                                     CGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGC                                                         >  SRR4302535.3122299/1‑94 (MQ=60)
                                      GTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCG                                                        >  SRR4302535.1251042/1‑94 (MQ=60)
                                      GTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCG                                                        >  SRR4302535.1414017/1‑94 (MQ=60)
                                          AGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTT                                                    <  SRR4302535.2819927/94‑1 (MQ=60)
                                              CGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAA                                                <  SRR4302535.4268674/94‑1 (MQ=60)
                                              CGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAA                                                >  SRR4302535.3668248/1‑94 (MQ=60)
                                              CGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAA                                                <  SRR4302535.4211498/94‑1 (MQ=60)
                                              CGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAA                                                >  SRR4302535.2319743/1‑94 (MQ=60)
                                               GGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAAC                                               >  SRR4302535.2031279/1‑94 (MQ=60)
                                               GGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAAC                                               >  SRR4302535.4194589/1‑94 (MQ=60)
                                                GTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACC                                              >  SRR4302535.1863699/1‑94 (MQ=60)
                                                GTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACC                                              >  SRR4302535.2139597/1‑94 (MQ=60)
                                                GTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACC                                              <  SRR4302535.4142108/94‑1 (MQ=60)
                                                 TCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCG                                             >  SRR4302535.4672243/1‑94 (MQ=60)
                                                  CTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGC                                            >  SRR4302535.530379/1‑94 (MQ=60)
                                                   TTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCA                                           <  SRR4302535.2945821/94‑1 (MQ=60)
                                                   TTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCA                                           >  SRR4302535.2897069/1‑94 (MQ=60)
                                                      CCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCA                                        >  SRR4302535.932760/1‑94 (MQ=60)
                                                       CTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAG                                       <  SRR4302535.435882/94‑1 (MQ=60)
                                                        TCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGG                                      >  SRR4302535.756085/1‑94 (MQ=60)
                                                        TCACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGG                                      >  SRR4302535.3721333/1‑94 (MQ=60)
                                                         CACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGT                                     >  SRR4302535.3769310/1‑94 (MQ=60)
                                                         CACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGT                                     >  SRR4302535.3257456/1‑94 (MQ=60)
                                                          ACTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTA                                    <  SRR4302535.3202409/94‑1 (MQ=60)
                                                           CTGGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAG                                   >  SRR4302535.3368667/1‑94 (MQ=60)
                                                             GGCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCG                                 <  SRR4302535.2809279/94‑1 (MQ=60)
                                                              GCACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGC                                <  SRR4302535.3739303/94‑1 (MQ=60)
                                                               CACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCT                               >  SRR4302535.1366837/1‑94 (MQ=60)
                                                               CACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCT                               <  SRR4302535.74172/94‑1 (MQ=60)
                                                                ACAGTACATTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTG                              >  SRR4302535.2917206/1‑94 (MQ=60)
                                                                ACAGTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTG                              >  SRR4302535.339603/1‑94 (MQ=60)
                                                                   GTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTT                           <  SRR4302535.4504667/94‑1 (MQ=60)
                                                                   GTACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTT                           >  SRR4302535.3831796/1‑94 (MQ=60)
                                                                    TACATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTG                          >  SRR4302535.4030083/1‑94 (MQ=60)
                                                                      CATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAG                        <  SRR4302535.2476391/94‑1 (MQ=60)
                                                                      CATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAG                        <  SRR4302535.3807282/94‑1 (MQ=60)
                                                                      CATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAG                        >  SRR4302535.1535351/1‑94 (MQ=60)
                                                                      CATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAG                        <  SRR4302535.65343/94‑1 (MQ=60)
                                                                       ATTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGG                       >  SRR4302535.4058631/1‑94 (MQ=60)
                                                                       ATTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGG                       <  SRR4302535.341175/94‑1 (MQ=60)
                                                                       ATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGG                       >  SRR4302535.4168449/1‑94 (MQ=60)
                                                                       ATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGG                       >  SRR4302535.2011067/1‑94 (MQ=60)
                                                                       ATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGG                       >  SRR4302535.4496628/1‑94 (MQ=60)
                                                                       ATTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGG                       >  SRR4302535.2179984/1‑94 (MQ=60)
                                                                        TTGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGA                      <  SRR4302535.3669935/94‑1 (MQ=60)
                                                                         TGATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGAT                     >  SRR4302535.2363004/1‑94 (MQ=60)
                                                                          GATGATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATC                    <  SRR4302535.4033974/94‑1 (MQ=60)
                                                                             GATCCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGG                 <  SRR4302535.3908743/94‑1 (MQ=60)
                                                                                CCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCT              <  SRR4302535.115352/94‑1 (MQ=60)
                                                                                CCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCT              <  SRR4302535.967923/94‑1 (MQ=60)
                                                                                CCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCT              >  SRR4302535.994644/1‑94 (MQ=60)
                                                                                CCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCT              <  SRR4302535.3860005/94‑1 (MQ=60)
                                                                                CCAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCT              <  SRR4302535.224982/94‑1 (MQ=60)
                                                                                 CAGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTG             >  SRR4302535.1949602/1‑94 (MQ=60)
                                                                                  AGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGC            >  SRR4302535.3568905/1‑94 (MQ=60)
                                                                                  AGAAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGC            >  SRR4302535.1850823/1‑94 (MQ=60)
                                                                                    AAGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGC          >  SRR4302535.250921/1‑94 (MQ=60)
                                                                                     AGAAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCA         >  SRR4302535.3217741/1‑94 (MQ=60)
                                                                                       AAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAAT       <  SRR4302535.2685742/94‑1 (MQ=60)
                                                                                       AAGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAAT       >  SRR4302535.8289/1‑94 (MQ=60)
                                                                                        AGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATG      >  SRR4302535.4047097/1‑94 (MQ=60)
                                                                                        AGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATG      >  SRR4302535.4011899/1‑94 (MQ=60)
                                                                                        AGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATG      >  SRR4302535.634650/1‑94 (MQ=60)
                                                                                        AGTGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATG      >  SRR4302535.1780069/1‑94 (MQ=60)
                                                                                          TGCGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGAT    >  SRR4302535.424553/1‑94 (MQ=60)
                                                                                            CGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGT  <  SRR4302535.4210793/94‑1 (MQ=60)
                                                                                            CGGTTTGTGAAATCACCAAAATCGTTCCGCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGT  <  SRR4302535.556464/94‑1 (MQ=60)
                                                                                            |                                                                                             
ATAACCGCGAAGAAGATATCGATGTTACGGCGAAAAGCGTGCAGTTCGGTCTTTCCTCACTGGCACAGTACATTGATGATCCAGAAGAAGTGTGGTTTGTGAAATCACCAAAATCGTTCCTCGGTGCCAGCGGCTTAAAACCGCAGCAGGTAGCGCTGTTTGAGGATCTGGTCTGCGCAATGATGT  >  NC_000913/2145913‑2146098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑