Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,262,769	A→G	Y296C (TAC→TGC)	yeiO →	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,769	0	A	G	94.8%	478.0 / 12.1	172	Y296C (TAC→TGC)	yeiO	putative transport
Reads supporting (aligned to +/- strand): ref base A (2/7); new base G (57/106); total (59/113)
Fisher's exact test for biased strand distribution p-value = 7.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01

Only 100 of 174 total aligned reads displayed.

CCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCG  >  NC_000913/2262680‑2262858

cccGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGc                                                                                        <  1:56721/93‑1 (MQ=255)

  cgacgaTGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAg                                                                                      >  2:4104808/1‑93 (MQ=255)

     cgaTGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGaa                                                                                   <  1:2820007/93‑1 (MQ=255)

      gaTGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAAt                                                                                  >  1:4646880/1‑93 (MQ=255)

      gaTGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAAt                                                                                  >  1:2952319/1‑93 (MQ=255)

       aTGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATg                                                                                 >  2:490934/1‑93 (MQ=255)

        tGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGc                                                                                >  1:2315025/1‑93 (MQ=255)

          ttGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTg                                                                              >  2:1684044/1‑93 (MQ=255)

           tGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGa                                                                             <  1:1519693/93‑1 (MQ=255)

           tGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGa                                                                             <  2:3414477/93‑1 (MQ=255)

             aTTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATg                                                                           <  2:3918685/93‑1 (MQ=255)

             aTTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATg                                                                           <  1:1739609/93‑1 (MQ=255)

                gCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGgcg                                                                        >  2:1344268/1‑93 (MQ=255)

                  cGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCa                                                                      <  1:3455261/93‑1 (MQ=255)

                  cGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCa                                                                      <  1:2549960/93‑1 (MQ=255)

                   ggATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCAt                                                                     <  2:3978142/93‑1 (MQ=255)

                      tatTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCa                                                                  <  1:648052/93‑1 (MQ=255)

                      tatTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCa                                                                  <  1:4045283/93‑1 (MQ=255)

                      tatTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGGCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCa                                                                  <  1:835299/93‑1 (MQ=255)

                       atTTCGCCAAACGTCTGGGCAAGCGTTTCTTAATGCGGGATGCTGCCGCGGGTTGCGTCTGCTTTTGCGCAGGAATGCTGATGGCGGATTCAc                                                                 >  2:2520738/1‑93 (MQ=255)

                        tttCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCAcc                                                                >  2:1061907/1‑93 (MQ=255)

                         ttCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCt                                                               >  2:2651677/1‑93 (MQ=255)

                          tCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTg                                                              <  1:967962/93‑1 (MQ=255)

                            gCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTc                                                            <  2:317414/93‑1 (MQ=255)

                              cAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCAt                                                          >  2:2789367/1‑93 (MQ=255)

                               aaaCGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCAtt                                                         <  2:4634844/93‑1 (MQ=255)

                                    tCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGtt                                                    <  2:2595317/93‑1 (MQ=255)

                                    tCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGtt                                                    >  1:2042020/1‑93 (MQ=255)

                                    tCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGtc                                                    >  1:3161576/1‑92 (MQ=255)

                                    tCTGGGTAAGCGTTTCTTAATGCGCGTTCCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGtt                                                    <  2:3171470/93‑1 (MQ=255)

                                     cTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTg                                                   <  2:2756444/93‑1 (MQ=255)

                                     cTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTg                                                   <  1:4076500/93‑1 (MQ=255)

                                      tGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTgg                                                  <  1:443874/93‑1 (MQ=255)

                                      tGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTgg                                                  >  2:1510024/1‑93 (MQ=255)

                                      tGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTgg                                                  <  2:2542172/93‑1 (MQ=255)

                                      tGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTgg                                                  >  1:3021325/1‑93 (MQ=255)

                                      tGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTgg                                                  <  1:2200924/93‑1 (MQ=255)

                                      tGGGTAAGCGTTTCTTAACGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTgg                                                  <  1:850318/93‑1 (MQ=255)

                                         gTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCt                                               <  2:804612/93‑1 (MQ=255)

                                         gTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCt                                               <  1:1116338/93‑1 (MQ=255)

                                          tAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCtt                                              >  2:3206595/1‑93 (MQ=255)

                                          tAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCtt                                              <  1:734307/93‑1 (MQ=255)

                                          tAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCtt                                              >  1:3512354/1‑93 (MQ=255)

                                           aaGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTg                                             >  1:650816/1‑93 (MQ=255)

                                           aaGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTg                                             <  1:674997/93‑1 (MQ=255)

                                            aGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGc                                            <  1:3238556/93‑1 (MQ=255)

                                             gCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCa                                           <  1:3567108/93‑1 (MQ=255)

                                              cGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAg                                          <  2:2119258/93‑1 (MQ=255)

                                              cGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAg                                          <  2:4125051/93‑1 (MQ=255)

                                               gTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAgc                                         <  2:2060737/93‑1 (MQ=255)

                                               gTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAgc                                         >  1:620663/1‑93 (MQ=255)

                                               gTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAgc                                         <  1:3446293/93‑1 (MQ=255)

                                                 ttCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAgctg                                       >  2:2745640/1‑93 (MQ=255)

                                                  tCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAgctgc                                      <  1:2719965/93‑1 (MQ=255)

                                                    ttAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTa                                    <  2:2953310/93‑1 (MQ=255)

                                                    ttAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTa                                    <  2:4392924/93‑1 (MQ=255)

                                                     tAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTaa                                   >  1:1578847/1‑93 (MQ=255)

                                                     tAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTaa                                   >  1:3666981/1‑93 (MQ=255)

                                                      aaTGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTaaa                                  <  2:1705006/93‑1 (MQ=255)

                                                      aaTGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTaaa                                  <  1:3880543/93‑1 (MQ=255)

                                                      aaTGCGCGTTGCTGCCGTGAGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTaaa                                  >  2:4527107/1‑93 (MQ=255)

                                                       aTGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAAt                                 <  1:966122/93‑1 (MQ=255)

                                                       aTGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAAt                                 <  1:1264867/93‑1 (MQ=255)

                                                       aTGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAAt                                 <  1:742818/93‑1 (MQ=255)

                                                       aTGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAAt                                 <  2:605524/93‑1 (MQ=255)

                                                           gcgTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTa                             >  2:3638264/1‑93 (MQ=255)

                                                            cgTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTAt                            <  1:1338276/93‑1 (MQ=255)

                                                            cgTTGCTGCCGTGGGTGGCGTCTGTTGTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTAt                            <  1:4242811/93‑1 (MQ=255)

                                                             gTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTAtt                           >  1:3160241/1‑93 (MQ=255)

                                                              ttGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTAttt                          >  1:1163942/1‑93 (MQ=255)

                                                                 ctgcCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTa                       <  2:4514143/93‑1 (MQ=255)

                                                                   gcCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTAtt                     <  1:2775775/93‑1 (MQ=255)

                                                                   gcCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTAtt                     <  1:2385735/93‑1 (MQ=255)

                                                                   gcCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTAtt                     <  1:1908827/93‑1 (MQ=255)

                                                                    ccGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTg                    <  2:3520878/93‑1 (MQ=255)

                                                                    ccGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTg                    <  2:399461/93‑1 (MQ=255)

                                                                    ccGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTg                    <  1:1022435/93‑1 (MQ=255)

                                                                    ccGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTg                    <  2:2284838/93‑1 (MQ=255)

                                                                     cGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTgg                   <  2:3246316/93‑1 (MQ=255)

                                                                      gtgggtggCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGc                  <  1:922016/93‑1 (MQ=255)

                                                                          gtggCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTc              <  2:710865/93‑1 (MQ=255)

                                                                          gtggCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTc              >  2:1922210/1‑93 (MQ=255)

                                                                          gtggCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTc              <  1:780572/93‑1 (MQ=255)

                                                                           tggCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCt             <  1:905114/93‑1 (MQ=255)

                                                                            ggCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTg            <  2:66128/93‑1 (MQ=255)

                                                                            ggCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTg            <  1:3785384/93‑1 (MQ=255)

                                                                            ggCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTg            <  1:1221172/93‑1 (MQ=255)

                                                                             gCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTgg           <  1:2277878/93‑1 (MQ=255)

                                                                             gCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTgg           <  1:3577799/93‑1 (MQ=255)

                                                                             gCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTgg           <  1:1418157/93‑1 (MQ=255)

                                                                              cGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTggg          <  1:3873712/93‑1 (MQ=255)

                                                                               gTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGggc         <  1:4605673/93‑1 (MQ=255)

                                                                                tCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGggcg        >  1:69824/1‑93 (MQ=255)

                                                                                  tGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGggcggc      >  2:3416495/1‑93 (MQ=255)

                                                                                   gTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCa     >  2:4177684/1‑93 (MQ=255)

                                                                                    tttttGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGGAt    >  2:3922515/1‑93 (MQ=255)

                                                                                     ttttGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATc   <  1:3802535/93‑1 (MQ=255)

                                                                                     ttttGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATc   >  1:623067/1‑93 (MQ=255)

                                                                                      tttGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCg  <  1:4226995/93‑1 (MQ=255)

                                                                                      tttGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCg  >  1:240951/1‑93 (MQ=255)

CCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCG  >  NC_000913/2262680‑2262858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGAT  >  NC_000913/2262676‑2262862

AAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTG                                                                                               >  SRR4302535.2048177/1‑94 (MQ=60)

AAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTG                                                                                               >  SRR4302535.63372/1‑94 (MQ=60)

AAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTG                                                                                               >  SRR4302535.1502674/1‑94 (MQ=60)

    CCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCA                                                                                           <  SRR4302535.57132/94‑1 (MQ=60)

      CGACGATGTTTATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGG                                                                                         <  SRR4302535.1980382/94‑1 (MQ=60)

         CGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAAT                                                                                      <  SRR4302535.464670/94‑1 (MQ=60)

         CGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAAT                                                                                      >  SRR4302535.4678362/1‑94 (MQ=60)

         CGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAAT                                                                                      >  SRR4302535.2973060/1‑94 (MQ=60)

         CGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAAT                                                                                      <  SRR4302535.2840036/94‑1 (MQ=60)

         CGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAAT                                                                                      <  SRR4302535.970578/94‑1 (MQ=60)

         CGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAAT                                                                                      >  SRR4302535.2498701/1‑94 (MQ=60)

           ATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGC                                                                                    >  SRR4302535.2702758/1‑94 (MQ=60)

           ATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGC                                                                                    >  SRR4302535.1197416/1‑94 (MQ=60)

           ATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGC                                                                                    >  SRR4302535.2331589/1‑94 (MQ=60)

             GTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTG                                                                                  <  SRR4302535.820118/94‑1 (MQ=60)

               TGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGAT                                                                                <  SRR4302535.1530639/94‑1 (MQ=60)

               TGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGAT                                                                                <  SRR4302535.914970/94‑1 (MQ=60)

               TGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGAT                                                                                <  SRR4302535.1313180/94‑1 (MQ=60)

                GATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATG                                                                               <  SRR4302535.2776530/94‑1 (MQ=60)

                 ATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGG                                                                              <  SRR4302535.1752178/94‑1 (MQ=60)

                      CGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCAT                                                                         <  SRR4302535.2568317/94‑1 (MQ=60)

                      CGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCAT                                                                         <  SRR4302535.3479277/94‑1 (MQ=60)

                          TATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCAC                                                                     <  SRR4302535.652570/94‑1 (MQ=60)

                          TATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCAC                                                                     <  SRR4302535.4072977/94‑1 (MQ=60)

                          TATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGGCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCAC                                                                     <  SRR4302535.841157/94‑1 (MQ=60)

                            TTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCT                                                                   <  SRR4302535.689746/94‑1 (MQ=60)

                              TCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGT                                                                 <  SRR4302535.974841/94‑1 (MQ=60)

                                 CCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCAT                                                              <  SRR4302535.626981/94‑1 (MQ=60)

                                   AAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTC                                                            >  SRR4302535.4627406/1‑94 (MQ=60)

                                    AACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCT                                                           <  SRR4302535.3539888/94‑1 (MQ=60)

                                     ACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTG                                                          <  SRR4302535.3315840/94‑1 (MQ=60)

                                       GTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTT                                                        >  SRR4302535.2056731/1‑94 (MQ=60)

                                       GTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTC                                                        >  SRR4302535.3183506/1‑94 (MQ=60)

                                         CTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGG                                                      <  SRR4302535.4104392/94‑1 (MQ=60)

                                         CTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGG                                                      >  SRR4302535.725943/1‑94 (MQ=60)

                                         CTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGG                                                      >  SRR4302535.3042482/1‑94 (MQ=60)

                                          TGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGG                                                     >  SRR4302535.4099725/1‑94 (MQ=60)

                                          TGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGG                                                     <  SRR4302535.446982/94‑1 (MQ=60)

                                          TGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGG                                                     <  SRR4302535.2216613/94‑1 (MQ=60)

                                          TGGGTAAGCGTTTCTTAACGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGG                                                     <  SRR4302535.856305/94‑1 (MQ=60)

                                             GTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTT                                                  <  SRR4302535.4442094/94‑1 (MQ=60)

                                             GTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTT                                                  >  SRR4302535.3536760/1‑94 (MQ=60)

                                             GTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTT                                                  <  SRR4302535.1124297/94‑1 (MQ=60)

                                              TAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTG                                                 <  SRR4302535.739467/94‑1 (MQ=60)

                                              TAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTG                                                 >  SRR4302535.655355/1‑94 (MQ=60)

                                               AAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGC                                                <  SRR4302535.679715/94‑1 (MQ=60)

                                                AGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCA                                               <  SRR4302535.3261041/94‑1 (MQ=60)

                                                 GCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAG                                              <  SRR4302535.176490/94‑1 (MQ=60)

                                                 GCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAG                                              <  SRR4302535.3591835/94‑1 (MQ=60)

                                                 GCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAG                                              <  SRR4302535.2364994/94‑1 (MQ=60)

                                                  CGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCCGC                                             >  SRR4302535.639245/1‑94 (MQ=60)

                                                  CGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGC                                             >  SRR4302535.624976/1‑94 (MQ=60)

                                                   GTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCT                                            <  SRR4302535.3591970/94‑1 (MQ=60)

                                                   GTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCT                                            <  SRR4302535.3470258/94‑1 (MQ=60)

                                                   GTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCT                                            <  SRR4302535.671087/94‑1 (MQ=60)

                                                   GTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCT                                            <  SRR4302535.227645/94‑1 (MQ=60)

                                                      TCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCT                                         <  SRR4302535.2739396/94‑1 (MQ=60)

                                                        TTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAA                                       >  SRR4302535.1590245/1‑94 (MQ=60)

                                                        TTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAA                                       >  SRR4302535.3692374/1‑94 (MQ=60)

                                                          AATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAAT                                     <  SRR4302535.3834304/94‑1 (MQ=60)

                                                          AATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAAT                                     <  SRR4302535.3147268/94‑1 (MQ=60)

                                                          AATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAAT                                     <  SRR4302535.3907178/94‑1 (MQ=60)

                                                           ATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATG                                    <  SRR4302535.748033/94‑1 (MQ=60)

                                                           ATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATG                                    <  SRR4302535.1273915/94‑1 (MQ=60)

                                                           ATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATG                                    <  SRR4302535.972987/94‑1 (MQ=60)

                                                              CGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTA                                 >  SRR4302535.1651875/1‑94 (MQ=60)

                                                                CGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATT                               >  SRR4302535.3182167/1‑94 (MQ=60)

                                                                CGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATT                               <  SRR4302535.350032/94‑1 (MQ=60)

                                                                CGTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATT                               <  SRR4302535.1347824/94‑1 (MQ=60)

                                                                CGTTGCTGCCGTGGGTGGCGTCTGTTGTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATT                               <  SRR4302535.4271851/94‑1 (MQ=60)

                                                                 GTTGCTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTT                              >  SRR4302535.1172255/1‑94 (MQ=60)

                                                                     CTGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTAT                          <  SRR4302535.4162174/94‑1 (MQ=60)

                                                                      TGCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATT                         <  SRR4302535.857750/94‑1 (MQ=60)

                                                                       GCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTG                        <  SRR4302535.2795537/94‑1 (MQ=60)

                                                                       GCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTG                        <  SRR4302535.2402880/94‑1 (MQ=60)

                                                                       GCCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTG                        <  SRR4302535.1922587/94‑1 (MQ=60)

                                                                        CCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGG                       <  SRR4302535.1029699/94‑1 (MQ=60)

                                                                        CCGTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGG                       <  SRR4302535.2865048/94‑1 (MQ=60)

                                                                          GTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCA                     >  SRR4302535.3700690/1‑94 (MQ=60)

                                                                          GTGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCA                     <  SRR4302535.928552/94‑1 (MQ=60)

                                                                           TGGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCAT                    <  SRR4302535.3002109/94‑1 (MQ=60)

                                                                            GGGTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATT                   <  SRR4302535.748566/94‑1 (MQ=60)

                                                                              GTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCT                 <  SRR4302535.786051/94‑1 (MQ=60)

                                                                              GTGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCT                 <  SRR4302535.1411233/94‑1 (MQ=60)

                                                                               TGGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTG                <  SRR4302535.911538/94‑1 (MQ=60)

                                                                                GGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGG               <  SRR4302535.3811496/94‑1 (MQ=60)

                                                                                GGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGG               <  SRR4302535.2300827/94‑1 (MQ=60)

                                                                                GGCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGG               <  SRR4302535.1229922/94‑1 (MQ=60)

                                                                                 GCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGG              <  SRR4302535.1428282/94‑1 (MQ=60)

                                                                                 GCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGG              <  SRR4302535.3602602/94‑1 (MQ=60)

                                                                                 GCGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGG              <  SRR4302535.2294147/94‑1 (MQ=60)

                                                                                  CGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGC             >  SRR4302535.3815923/1‑94 (MQ=60)

                                                                                  CGTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGC             <  SRR4302535.3900330/94‑1 (MQ=60)

                                                                                   GTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCG            >  SRR4302535.70336/1‑94 (MQ=60)

                                                                                   GTCTGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCG            <  SRR4302535.4636978/94‑1 (MQ=60)

                                                                                      TGTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCA         <  SRR4302535.1160748/94‑1 (MQ=60)

                                                                                       GTTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCAT        >  SRR4302535.3229001/1‑94 (MQ=60)

                                                                                        TTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATC       >  SRR4302535.3994871/1‑94 (MQ=60)

                                                                                        TTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATC       <  SRR4302535.1528361/94‑1 (MQ=60)

                                                                                        TTTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATC       >  SRR4302535.627396/1‑94 (MQ=60)

                                                                                         TTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCG      <  SRR4302535.3828747/94‑1 (MQ=60)

                                                                                         TTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCG      >  SRR4302535.242602/1‑94 (MQ=60)

                                                                                         TTTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCG      >  SRR4302535.579528/1‑94 (MQ=60)

                                                                                          TTTGCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGG     <  SRR4302535.4255921/94‑1 (MQ=60)

                                                                                             GCGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGAT  <  SRR4302535.732725/94‑1 (MQ=60)

AAATCCCGACGATGTTGATTGCCGGATATTTCGCCAAACGTCTGGGTAAGCGTTTCTTAATGCGCGTTGCTGCCGTGGGTGGCGTCTGTTTTTACGCAGGAATGCTGATGGCGCATTCACCTGTCATTCTGTTGGGCTTGCAGCTGCTAAATGCTATTTTTATTGGCATTCTGGGCGGCATCGGGAT  >  NC_000913/2262676‑2262862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`