Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,962,096 T→C Q35R (CAG→CGG)  ppdA ← prepilin peptidase dependent protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,962,0960TC99.4% 491.9 / NA 159Q35R (CAG→CGG) ppdAprepilin peptidase dependent protein A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (89/69);  minor base A (1/0);  total (90/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

CTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACTGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCA  >  NC_000913/2962006‑2962185
                                                                                          |                                                                                         
cTGTGGACGCGGTTATGCCAGTTGGCATCTTCACCTAAATAGAGCAAATAGTCCAGCGCCTGGCTGGCGGTTTGCCATAGCCGGTGCGAccgc                                                                                         >  2:4060772/1‑93 (MQ=255)
 tgtgGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGAccgct                                                                                        <  2:241968/93‑1 (MQ=255)
     gTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCa                                                                                    >  1:452669/1‑93 (MQ=255)
     gTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCa                                                                                    >  2:3231933/1‑93 (MQ=255)
          ggTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTAtt                                                                               <  2:1466677/93‑1 (MQ=255)
          ggTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTAtt                                                                               <  2:358278/93‑1 (MQ=255)
           gTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTg                                                                              <  2:117703/93‑1 (MQ=255)
            ttATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgc                                                                             >  1:2156461/1‑93 (MQ=255)
            ttATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgc                                                                             >  2:1021048/1‑93 (MQ=255)
            ttATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgc                                                                             <  1:1807505/93‑1 (MQ=255)
            ttATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgc                                                                             <  2:1075940/93‑1 (MQ=255)
              aTGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgcca                                                                           >  1:4259407/1‑93 (MQ=255)
              aTGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgcca                                                                           >  1:1171944/1‑93 (MQ=255)
              aTGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgcca                                                                           <  1:2094128/93‑1 (MQ=255)
              aTGCCAGTTGGCATCTTCACATAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgcca                                                                           >  2:3832254/1‑93 (MQ=255)
                gCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgccagc                                                                         >  2:2770218/1‑93 (MQ=255)
                  cAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTgccagcca                                                                       >  1:49913/1‑93 (MQ=255)
                    gTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATa                                                                     >  1:422341/1‑93 (MQ=255)
                    gTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATa                                                                     >  1:1416951/1‑93 (MQ=255)
                     ttGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATag                                                                    >  1:2905432/1‑93 (MQ=255)
                      tGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATaga                                                                   <  1:1055368/93‑1 (MQ=255)
                        gCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGt                                                                 >  1:736220/1‑93 (MQ=255)
                        gCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGt                                                                 <  2:3293844/93‑1 (MQ=255)
                         cATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTc                                                                <  2:4064932/93‑1 (MQ=255)
                           tCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCa                                                              >  1:87762/1‑93 (MQ=255)
                           tCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCa                                                              >  1:3074533/1‑93 (MQ=255)
                           tCTTCACGTAAAAAGAGCAAATCGTCCCGCGCCTGACTGACGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCa                                                              <  1:3208003/93‑1 (MQ=255)
                            cTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCAc                                                             <  2:743987/93‑1 (MQ=255)
                             ttCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACAGCTGCCAGTATTGCCAGCCATAGAGTCCACt                                                            >  1:1331651/1‑93 (MQ=255)
                                   tAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCt                                                      >  2:1776054/1‑93 (MQ=255)
                                   tAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCt                                                      >  1:764329/1‑93 (MQ=255)
                                    aaaTAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCtt                                                     >  1:258921/1‑93 (MQ=255)
                                    aaaTAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCtt                                                     <  2:3522952/93‑1 (MQ=255)
                                     aaTAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTa                                                    <  2:1373277/93‑1 (MQ=255)
                                      aTAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAg                                                   >  2:3517790/1‑93 (MQ=255)
                                      aTAAAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAg                                                   <  1:2345829/93‑1 (MQ=255)
                                       tAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGc                                                  >  1:3497597/1‑93 (MQ=255)
                                         gagCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCAt                                                >  1:3739389/1‑93 (MQ=255)
                                           gCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGa                                              >  2:888677/1‑93 (MQ=255)
                                             aaaTAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGAcc                                            >  2:49016/1‑93 (MQ=255)
                                              aaTAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCa                                           >  2:2731182/1‑93 (MQ=255)
                                               aTAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCaa                                          <  2:1998919/93‑1 (MQ=255)
                                               aTAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGAATGACCaa                                          >  1:3885354/1‑93 (MQ=255)
                                                tAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCaaa                                         >  2:337395/1‑93 (MQ=255)
                                                tAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCaaa                                         >  2:3079243/1‑93 (MQ=255)
                                                  gTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAAt                                       <  2:1705836/93‑1 (MQ=255)
                                                  gTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAAt                                       >  1:2594011/1‑93 (MQ=255)
                                                  gTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAAt                                       >  1:1442042/1‑93 (MQ=255)
                                                  gTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAAt                                       >  2:3295413/1‑93 (MQ=255)
                                                   tCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATc                                      <  2:1452550/93‑1 (MQ=255)
                                                   tCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATc                                      <  1:1239555/93‑1 (MQ=255)
                                                    cccGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCa                                     <  1:1685003/93‑1 (MQ=255)
                                                     ccGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAg                                    >  1:486159/1‑93 (MQ=255)
                                                     ccGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAg                                    >  2:3710335/1‑93 (MQ=255)
                                                       gcgcCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCa                                  <  1:3556620/93‑1 (MQ=255)
                                                       gcgcCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCa                                  >  2:3711671/1‑93 (MQ=255)
                                                       gcgcCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCa                                  <  2:3927272/93‑1 (MQ=255)
                                                       gcgcCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCa                                  <  1:833801/93‑1 (MQ=255)
                                                        cgcCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCAt                                 >  2:2941852/1‑93 (MQ=255)
                                                         gcCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATc                                <  1:966804/93‑1 (MQ=255)
                                                         gcCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATc                                <  1:4436118/93‑1 (MQ=255)
                                                          ccTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATcg                               >  1:1362241/1‑93 (MQ=255)
                                                          ccTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATcg                               >  1:2377178/1‑93 (MQ=255)
                                                          ccTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATcg                               >  2:313255/1‑93 (MQ=255)
                                                           ctggctggCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATcgc                              >  2:1922893/1‑93 (MQ=255)
                                                           ctggctggCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATcgc                              <  1:4092570/93‑1 (MQ=255)
                                                           ctggctggCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATcgc                              >  1:1026959/1‑93 (MQ=255)
                                                            tggctggcGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATcgcg                             >  1:2988647/1‑93 (MQ=255)
                                                            tggctggcGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATcgcg                             >  1:555691/1‑93 (MQ=255)
                                                             ggctggcGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGa                            >  1:4173229/1‑93 (MQ=255)
                                                               ctggcGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCTTCGCGAcc                          <  2:4584636/93‑1 (MQ=255)
                                                                tggcGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCa                         >  1:3252102/1‑93 (MQ=255)
                                                                 ggcGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAg                        <  1:4590000/93‑1 (MQ=255)
                                                                    gttttGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCGCTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGt                     <  1:1381233/91‑1 (MQ=255)
                                                                    ggTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGt                     >  1:4397723/1‑93 (MQ=255)
                                                                     gTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGtt                    >  2:4549943/1‑93 (MQ=255)
                                                                     gTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGtt                    >  1:225782/1‑93 (MQ=255)
                                                                     gTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGtt                    >  1:1498920/1‑93 (MQ=255)
                                                                     gTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGtt                    >  1:4521591/1‑93 (MQ=255)
                                                                      tttGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGttt                   >  1:1277656/1‑93 (MQ=255)
                                                                      tttACCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGttt                   <  2:2387848/93‑1 (MQ=255)
                                                                       ttGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTc                  <  2:4302721/93‑1 (MQ=255)
                                                                       ttGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTc                  <  1:2974667/93‑1 (MQ=255)
                                                                         gCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCaa                <  1:4217953/93‑1 (MQ=255)
                                                                         gCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCaa                <  2:2288712/93‑1 (MQ=255)
                                                                         gCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCaa                <  2:248793/93‑1 (MQ=255)
                                                                          ccATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAAt               >  2:4668997/1‑93 (MQ=255)
                                                                           cATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATc              <  2:3335250/93‑1 (MQ=255)
                                                                           cATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATc              <  1:2748156/93‑1 (MQ=255)
                                                                           cATAGCCGTGGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATc              <  1:188164/93‑1 (MQ=255)
                                                                              aGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGc           <  1:3707826/93‑1 (MQ=255)
                                                                               gcggTTGCGACCGCGGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCAGGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCg          <  1:1951601/90‑1 (MQ=255)
                                                                               gCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCg          >  2:2559401/1‑93 (MQ=255)
                                                                               gCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCg          <  1:496635/93‑1 (MQ=255)
                                                                                 cGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGta        <  2:96550/93‑1 (MQ=255)
                                                                                    tGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATaa     <  2:2683847/93‑1 (MQ=255)
                                                                                      cGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAAcc   <  1:1244714/93‑1 (MQ=255)
                                                                                      cGACCGCTGCCAGTATGGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAAcc   <  2:741166/93‑1 (MQ=255)
                                                                                       gACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCa  >  2:2633059/1‑93 (MQ=255)
                                                                                       gACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCa  <  2:2262938/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACTGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCA  >  NC_000913/2962006‑2962185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACTGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCACGTT  >  NC_000913/2962004‑2962189
                                                                                            |                                                                                             
TACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCG                                                                                              >  SRR4302535.965754/1‑94 (MQ=60)
 ACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGC                                                                                             >  SRR4302535.279361/1‑94 (MQ=60)
 ACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGC                                                                                             >  SRR4302535.2958170/1‑94 (MQ=60)
      GGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCA                                                                                        >  SRR4302535.455839/1‑94 (MQ=60)
        TCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGT                                                                                      <  SRR4302535.494736/94‑1 (MQ=60)
        TCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGT                                                                                      >  SRR4302535.1365578/1‑94 (MQ=60)
         CGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTA                                                                                     <  SRR4302535.1882042/94‑1 (MQ=60)
           CGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATT                                                                                   >  SRR4302535.2017828/1‑94 (MQ=60)
           CGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATT                                                                                   <  SRR4302535.934674/94‑1 (MQ=60)
             GTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGC                                                                                 >  SRR4302535.2171955/1‑94 (MQ=60)
              TTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCC                                                                                <  SRR4302535.1820587/94‑1 (MQ=60)
               TATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCA                                                                               >  SRR4302535.4288581/1‑94 (MQ=60)
               TATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCA                                                                               >  SRR4302535.1180314/1‑94 (MQ=60)
                ATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAG                                                                              <  SRR4302535.2109226/94‑1 (MQ=60)
                   CCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCA                                                                           >  SRR4302535.50283/1‑94 (MQ=60)
                     AGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATA                                                                         >  SRR4302535.4685803/1‑94 (MQ=60)
                     AGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATA                                                                         >  SRR4302535.425289/1‑94 (MQ=60)
                     AGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATA                                                                         >  SRR4302535.1427067/1‑94 (MQ=60)
                      GTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAG                                                                        >  SRR4302535.2925969/1‑94 (MQ=60)
                        TGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAG                                                                      <  SRR4302535.2136778/94‑1 (MQ=60)
                        TGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAG                                                                      <  SRR4302535.1062872/94‑1 (MQ=60)
                         GGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGT                                                                     >  SRR4302535.741397/1‑94 (MQ=60)
                            ATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCA                                                                  >  SRR4302535.3095992/1‑94 (MQ=60)
                            ATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCA                                                                  >  SRR4302535.88378/1‑94 (MQ=60)
                            ATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCA                                                                  <  SRR4302535.1208377/94‑1 (MQ=60)
                             TCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCAC                                                                 <  SRR4302535.2571185/94‑1 (MQ=60)
                             TCTTCACGTAAAAAGAGCAAATCGTCCCGCGCCTGACTGACGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCAC                                                                 <  SRR4302535.3230223/94‑1 (MQ=60)
                              CTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACAGCTGCCAGTATTGCCAGCCATAGAGTCCACT                                                                >  SRR4302535.1341152/1‑94 (MQ=60)
                                TCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTG                                                              >  SRR4302535.4250129/1‑94 (MQ=60)
                                  ACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCG                                                            <  SRR4302535.3932653/94‑1 (MQ=60)
                                    GTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCT                                                          >  SRR4302535.846397/1‑94 (MQ=60)
                                    GTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCT                                                          >  SRR4302535.769712/1‑94 (MQ=60)
                                     TAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTT                                                         >  SRR4302535.260712/1‑94 (MQ=60)
                                       AATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAG                                                       <  SRR4302535.2387779/94‑1 (MQ=60)
                                       AATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAG                                                       <  SRR4302535.4276294/94‑1 (MQ=60)
                                        ATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGC                                                      >  SRR4302535.3521901/1‑94 (MQ=60)
                                        ATAAAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGC                                                      <  SRR4302535.2362640/94‑1 (MQ=60)
                                          AGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCAT                                                    >  SRR4302535.1820565/1‑94 (MQ=60)
                                          AGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCAT                                                    >  SRR4302535.3765270/1‑94 (MQ=60)
                                          AGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCAT                                                    >  SRR4302535.4487108/1‑94 (MQ=60)
                                          AGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCAT                                                    >  SRR4302535.1447690/1‑94 (MQ=60)
                                               AAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCA                                               >  SRR4302535.691007/1‑94 (MQ=60)
                                               AAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATCACCA                                               >  SRR4302535.74218/1‑94 (MQ=60)
                                                AATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGAATGACCAA                                              >  SRR4302535.3912005/1‑94 (MQ=60)
                                                  TAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAA                                            >  SRR4302535.2624618/1‑94 (MQ=60)
                                                   AGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAAT                                           >  SRR4302535.1452358/1‑94 (MQ=60)
                                                   AGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAAT                                           >  SRR4302535.2612644/1‑94 (MQ=60)
                                                     TCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCCCTTGCGCTTAGCATGACCAAAATCA                                         >  SRR4302535.1529774/1‑94 (MQ=60)
                                                     TCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCA                                         <  SRR4302535.1248403/94‑1 (MQ=60)
                                                      CCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAG                                        <  SRR4302535.1697205/94‑1 (MQ=60)
                                                      CCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAG                                        >  SRR4302535.489566/1‑94 (MQ=60)
                                                         GCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCAT                                     <  SRR4302535.839646/94‑1 (MQ=60)
                                                         GCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCAT                                     <  SRR4302535.3581289/94‑1 (MQ=60)
                                                         CCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCAT                                     <  SRR4302535.3150689/94‑1 (MQ=60)
                                                          CGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATC                                    <  SRR4302535.4617972/94‑1 (MQ=60)
                                                          CGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATC                                    >  SRR4302535.1398420/1‑94 (MQ=60)
                                                           GCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCG                                   >  SRR4302535.1371984/1‑94 (MQ=60)
                                                           GCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCG                                   <  SRR4302535.4466521/94‑1 (MQ=60)
                                                           GCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCG                                   >  SRR4302535.2394254/1‑94 (MQ=60)
                                                           GCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCG                                   <  SRR4302535.973673/94‑1 (MQ=60)
                                                            CCTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGC                                  >  SRR4302535.1034252/1‑94 (MQ=60)
                                                             CTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCG                                 >  SRR4302535.559586/1‑94 (MQ=60)
                                                             CTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCG                                 <  SRR4302535.4120576/94‑1 (MQ=60)
                                                             CTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCG                                 >  SRR4302535.1257182/1‑94 (MQ=60)
                                                             CTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCG                                 >  SRR4302535.3443970/1‑94 (MQ=60)
                                                             CTGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCG                                 >  SRR4302535.3009601/1‑94 (MQ=60)
                                                              TGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGA                                >  SRR4302535.4201793/1‑94 (MQ=60)
                                                              TGGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGA                                >  SRR4302535.546499/1‑94 (MQ=60)
                                                               GGCTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGAC                               >  SRR4302535.3066256/1‑94 (MQ=60)
                                                                 CTGGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCA                             >  SRR4302535.3274696/1‑94 (MQ=60)
                                                                   GGCGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGC                           <  SRR4302535.4621244/94‑1 (MQ=60)
                                                                     CGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGT                         >  SRR4302535.4427824/1‑94 (MQ=60)
                                                                     CGGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGT                         <  SRR4302535.4219432/94‑1 (MQ=60)
                                                                      GTTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCGCTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTT                        <  SRR4302535.1391084/94‑1 (MQ=60)
                                                                      GGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTT                        >  SRR4302535.1509703/1‑94 (MQ=60)
                                                                      GGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTT                        >  SRR4302535.227335/1‑94 (MQ=60)
                                                                      GGTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTT                        >  SRR4302535.4552576/1‑94 (MQ=60)
                                                                       GTTTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTT                       >  SRR4302535.1286781/1‑94 (MQ=60)
                                                                         TTGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCA                     <  SRR4302535.2995545/94‑1 (MQ=60)
                                                                          TGCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAA                    >  SRR4302535.356865/1‑94 (MQ=60)
                                                                           GCCATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAAT                   <  SRR4302535.4246818/94‑1 (MQ=60)
                                                                             CATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCA                 <  SRR4302535.2767745/94‑1 (MQ=60)
                                                                             CATAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCA                 >  SRR4302535.1170906/1‑94 (MQ=60)
                                                                             CATAGCCGTGGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCA                 <  SRR4302535.189467/94‑1 (MQ=60)
                                                                               TAGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGC               >  SRR4302535.235306/1‑94 (MQ=60)
                                                                                AGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCG              <  SRR4302535.1292891/94‑1 (MQ=60)
                                                                                AGCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCG              <  SRR4302535.3733501/94‑1 (MQ=60)
                                                                                 GCGGTTGCGACCGCGGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCAGGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGT             <  SRR4302535.1965646/94‑1 (MQ=60)
                                                                                 GCCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGT             <  SRR4302535.500120/94‑1 (MQ=60)
                                                                                  CCGTTGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTA            >  SRR4302535.3701366/1‑94 (MQ=60)
                                                                                      TGCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAAC        <  SRR4302535.3807679/94‑1 (MQ=60)
                                                                                       GCGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACC       >  SRR4302535.4430889/1‑94 (MQ=60)
                                                                                        CGACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCA      <  SRR4302535.1253595/94‑1 (MQ=60)
                                                                                          ACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCACG    >  SRR4302535.4517205/1‑94 (MQ=60)
                                                                                          ACCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCACG    <  SRR4302535.3018608/94‑1 (MQ=60)
                                                                                           CCGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCACGT   >  SRR4302535.4606352/1‑94 (MQ=60)
                                                                                            CGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCACGTT  <  SRR4302535.3646204/94‑1 (MQ=60)
                                                                                            CGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCACGTT  <  SRR4302535.4218859/94‑1 (MQ=60)
                                                                                            CGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCACGTG  >  SRR4302535.4065209/1‑94 (MQ=60)
                                                                                            |                                                                                             
TACTGTGGTCGCGGTTATGCCAGTTGGCATCTTCACGTAAATAGAGCAAATAGTCCCGCGCCTGGCTGGCGGTTTGCCATAGCCGTTGCGACTGCTGCCAGTATTGCCAGCCATAGAGTCCACTTGCGCTTAGCATGACCAAAATCAGCATCGCGACCAGCGTTTCAATCAGCGTATAACCACGTT  >  NC_000913/2962004‑2962189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑