Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,012,620 T→C G132G (GGA→GGG)  b2876 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,012,6200TC97.8% 537.6 / 0.4 183G132G (GGA→GGG) b2876hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base C (86/91);  total (90/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.77e-01

Only 100 of 195 total aligned reads displayed.

CAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGTCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCC  >  NC_000913/3012530‑3012709
                                                                                          |                                                                                         
cAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGccc                                                                                         <  2:2079211/93‑1 (MQ=255)
  aGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGccctc                                                                                       >  1:3428708/1‑93 (MQ=255)
  aGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGccctc                                                                                       >  1:2317521/1‑93 (MQ=255)
   ggTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGccctcc                                                                                      <  1:1122063/93‑1 (MQ=255)
   ggTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGccctcc                                                                                      >  2:80934/1‑93 (MQ=255)
    gTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTccc                                                                                     <  1:1237916/93‑1 (MQ=255)
    gTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTccc                                                                                     <  2:1838827/93‑1 (MQ=255)
    gTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTccc                                                                                     >  2:1649014/1‑93 (MQ=255)
      tGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCAt                                                                                   >  1:562626/1‑93 (MQ=255)
      tGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCAt                                                                                   >  1:3918906/1‑93 (MQ=255)
       gCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATc                                                                                  <  2:920437/93‑1 (MQ=255)
       gCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATc                                                                                  <  1:1756126/93‑1 (MQ=255)
         aTCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATcac                                                                                >  2:968128/1‑93 (MQ=255)
         aTCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATcac                                                                                >  1:1351129/1‑93 (MQ=255)
          tCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATcaca                                                                               >  2:4074544/1‑93 (MQ=255)
          tCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATcaca                                                                               >  2:2838871/1‑93 (MQ=255)
          tCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATcaca                                                                               >  1:719461/1‑93 (MQ=255)
             ggTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCa                                                                            >  1:1035530/1‑93 (MQ=255)
              gtgtTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCaa                                                                           >  1:1851709/1‑93 (MQ=255)
               tgtTACCCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAAt                                                                          <  2:3321753/93‑1 (MQ=255)
               tgtTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAAt                                                                          >  1:3842037/1‑93 (MQ=255)
                 ttAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGCCATTTTCTGTGCTCACTTTCGTGCCCAAAGTATGCACTCCCATCACAGCAATCa                                                                        >  2:393441/1‑93 (MQ=255)
                  tAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCAc                                                                       <  1:4259285/93‑1 (MQ=255)
                    aCCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACgc                                                                     >  2:2440472/1‑93 (MQ=255)
                     cccGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACgca                                                                    >  2:3493489/1‑93 (MQ=255)
                      ccGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACgcag                                                                   >  1:1798280/1‑93 (MQ=255)
                      ccGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACgcag                                                                   >  2:25589/1‑93 (MQ=255)
                       cGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACgcagc                                                                  >  2:2626596/1‑93 (MQ=255)
                       cGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACgcagc                                                                  <  2:2111944/93‑1 (MQ=255)
                       cGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCCCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACgcagc                                                                  >  1:452743/1‑93 (MQ=255)
                        ggTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACgcagca                                                                 >  2:470281/1‑93 (MQ=255)
                        ggTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGACCTCCCATCACAGCAATCACgcagca                                                                 >  1:1681811/1‑93 (MQ=255)
                         gTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCaa                                                                >  1:4449132/1‑93 (MQ=255)
                           aaTATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACt                                                              <  1:2688742/93‑1 (MQ=255)
                            atatCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTg                                                             <  2:3432288/93‑1 (MQ=255)
                             tatCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCCACTGc                                                            >  1:1899581/1‑93 (MQ=255)
                             tatCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGc                                                            >  2:1252001/1‑93 (MQ=255)
                             tatCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGc                                                            <  1:2283018/93‑1 (MQ=255)
                               tCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCtt                                                          >  2:317809/1‑93 (MQ=255)
                                  gCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTg                                                       >  1:1207077/1‑93 (MQ=255)
                                  gCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTg                                                       >  2:187603/1‑93 (MQ=255)
                                  gCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTg                                                       <  2:1885609/93‑1 (MQ=255)
                                    aaaCTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTggg                                                     <  1:2805452/93‑1 (MQ=255)
                                      aCTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTa                                                   <  2:1598229/93‑1 (MQ=255)
                                      aCTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTa                                                   <  2:179021/93‑1 (MQ=255)
                                        tGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATg                                                 <  2:2104463/93‑1 (MQ=255)
                                         gcgcCCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGc                                                <  2:3669464/93‑1 (MQ=255)
                                         gCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGc                                                >  1:813416/1‑93 (MQ=255)
                                         gCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGc                                                <  1:1803516/93‑1 (MQ=255)
                                         gCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGc                                                >  2:4023330/1‑93 (MQ=255)
                                         gCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGc                                                <  1:4672808/93‑1 (MQ=255)
                                           gACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCaa                                              <  1:2998969/93‑1 (MQ=255)
                                           gACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCaa                                              <  1:4621205/93‑1 (MQ=255)
                                               aGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGtt                                          <  1:2475467/93‑1 (MQ=255)
                                               aGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGtt                                          >  2:3142124/1‑93 (MQ=255)
                                               aGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGtt                                          <  2:1804744/93‑1 (MQ=255)
                                                 cGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCg                                        <  2:4180915/93‑1 (MQ=255)
                                                  gATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGt                                       <  2:330263/93‑1 (MQ=255)
                                                  gATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGt                                       >  1:3938249/1‑93 (MQ=255)
                                                   aTGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTg                                      >  2:844791/1‑93 (MQ=255)
                                                    tGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGc                                     <  2:2653061/93‑1 (MQ=255)
                                                    tGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGc                                     >  1:3010746/1‑93 (MQ=255)
                                                    tGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGc                                     <  1:597791/93‑1 (MQ=255)
                                                     tgACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCt                                    <  2:544893/92‑1 (MQ=255)
                                                        cATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCtcat                                 >  2:2250454/1‑93 (MQ=255)
                                                         aTTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCtcatc                                >  2:1098823/1‑93 (MQ=255)
                                                         aTTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCtcatc                                <  2:3894664/93‑1 (MQ=255)
                                                          ttttCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCtcatca                               >  1:910671/1‑93 (MQ=255)
                                                          ttttCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCtcatca                               >  1:1744108/1‑93 (MQ=255)
                                                           tttCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAg                              <  1:779008/93‑1 (MQ=255)
                                                           tttCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAg                              <  1:3085308/93‑1 (MQ=255)
                                                            ttCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAgg                             <  1:2936838/93‑1 (MQ=255)
                                                                gtgCTCACTTTCGCGCCCAAAGTATGTCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCt                         >  1:1493228/1‑93 (MQ=255)
                                                                gtgCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCt                         <  2:1195735/93‑1 (MQ=255)
                                                                  gCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCttt                       <  2:2425823/93‑1 (MQ=255)
                                                                  gCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCttt                       <  1:2056660/93‑1 (MQ=255)
                                                                     cACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTaa                    <  1:1453344/93‑1 (MQ=255)
                                                                     cACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTaa                    <  1:2178291/93‑1 (MQ=255)
                                                                      aCTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAAc                   <  2:329249/93‑1 (MQ=255)
                                                                       cTTTCGCGCGCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACg                  <  2:4482460/93‑1 (MQ=255)
                                                                       cTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACg                  <  1:3401029/93‑1 (MQ=255)
                                                                        tttCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACgg                 >  2:4399793/1‑93 (MQ=255)
                                                                          tCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCACCTGCTTTTGGGTATGCAAGGTTCGTGCTCGTCAGGCGCTTTTAACGGCa               <  2:4569919/93‑1 (MQ=255)
                                                                           cgcgCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCAt              <  2:1919936/93‑1 (MQ=255)
                                                                             cgcCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTc            >  1:3283431/1‑93 (MQ=255)
                                                                             cgcCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTc            <  1:4143391/93‑1 (MQ=255)
                                                                              gcCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTcc           >  1:2849273/1‑93 (MQ=255)
                                                                               cccAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTGTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCa          <  1:3800083/93‑1 (MQ=255)
                                                                                ccAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCAc         <  2:2330788/93‑1 (MQ=255)
                                                                                ccAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCAc         >  2:1713525/1‑93 (MQ=255)
                                                                                ccAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCAc         >  2:1608214/1‑93 (MQ=255)
                                                                                ccAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCAc         <  2:3004524/93‑1 (MQ=255)
                                                                                  aaaGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCAcgc       <  1:1215521/93‑1 (MQ=255)
                                                                                   aaGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCAcgcg      >  2:144209/1‑93 (MQ=255)
                                                                                   aaGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCAcgcg      >  1:4191923/1‑93 (MQ=255)
                                                                                   aaGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCAcgcg      >  1:2086189/1‑93 (MQ=255)
                                                                                    aGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGa     <  1:3678570/93‑1 (MQ=255)
                                                                                     gTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAg    >  1:1338839/1‑93 (MQ=255)
                                                                                      tATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGc   <  1:2574149/93‑1 (MQ=255)
                                                                                       aTGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGcc  <  1:3768619/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGTCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCC  >  NC_000913/3012530‑3012709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGTCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCATCT  >  NC_000913/3012528‑3012713
                                                                                            |                                                                                             
TGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGGATGCC                                                                                              >  SRR4302535.824897/1‑94 (MQ=60)
  CAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCT                                                                                            <  SRR4302535.1739220/94‑1 (MQ=60)
   AAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTC                                                                                           >  SRR4302535.3452540/1‑94 (MQ=60)
   AAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTC                                                                                           >  SRR4302535.2334109/1‑94 (MQ=60)
     GGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCC                                                                                         <  SRR4302535.1130069/94‑1 (MQ=60)
      GTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCA                                                                                        <  SRR4302535.1246755/94‑1 (MQ=60)
      GTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCA                                                                                        <  SRR4302535.3447221/94‑1 (MQ=60)
       TTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCAT                                                                                       >  SRR4302535.566561/1‑94 (MQ=60)
       TTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCAT                                                                                       >  SRR4302535.3945710/1‑94 (MQ=60)
        TGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATC                                                                                      >  SRR4302535.818887/1‑94 (MQ=60)
         GCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCA                                                                                     <  SRR4302535.1768821/94‑1 (MQ=60)
          CATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCAC                                                                                    >  SRR4302535.602996/1‑94 (MQ=60)
          CATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCAC                                                                                    >  SRR4302535.1249336/1‑94 (MQ=60)
          CATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCAC                                                                                    >  SRR4302535.1360803/1‑94 (MQ=60)
           ATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACA                                                                                   >  SRR4302535.724518/1‑94 (MQ=60)
            TCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGTCCTCCCATCACAG                                                                                  >  SRR4302535.4659371/1‑94 (MQ=60)
            TCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAG                                                                                  <  SRR4302535.2088729/94‑1 (MQ=60)
              AGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCA                                                                                <  SRR4302535.4466793/94‑1 (MQ=60)
              AGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCA                                                                                >  SRR4302535.1042884/1‑94 (MQ=60)
              AGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCA                                                                                >  SRR4302535.2807431/1‑94 (MQ=60)
               GGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAA                                                                               >  SRR4302535.3335445/1‑94 (MQ=60)
               GGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAA                                                                               >  SRR4302535.1865051/1‑94 (MQ=60)
                GTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAAT                                                                              >  SRR4302535.3868505/1‑94 (MQ=60)
                   TTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCAC                                                                           >  SRR4302535.1671222/1‑94 (MQ=60)
                   TTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCAC                                                                           <  SRR4302535.3844666/94‑1 (MQ=60)
                   TTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCAC                                                                           <  SRR4302535.4596217/94‑1 (MQ=60)
                    TAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACG                                                                          <  SRR4302535.4288459/94‑1 (MQ=60)
                      ACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGTCCAAAGTATGCCCTCCCATCACAGCAATCACGCA                                                                        >  SRR4302535.3021133/1‑94 (MQ=60)
                       CCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAG                                                                       >  SRR4302535.1811283/1‑94 (MQ=60)
                        CCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCCCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGC                                                                      >  SRR4302535.455913/1‑94 (MQ=60)
                         CGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCA                                                                     <  SRR4302535.2564034/94‑1 (MQ=60)
                         CGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCA                                                                     <  SRR4302535.3002010/94‑1 (MQ=60)
                         CGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGACCTCCCATCACAGCAATCACGCAGCA                                                                     >  SRR4302535.1693990/1‑94 (MQ=60)
                          GGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAA                                                                    >  SRR4302535.4479649/1‑94 (MQ=60)
                          GGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAA                                                                    >  SRR4302535.2667576/1‑94 (MQ=60)
                             AATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTG                                                                 <  SRR4302535.2707955/94‑1 (MQ=60)
                              ATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCCACTGC                                                                >  SRR4302535.1913251/1‑94 (MQ=60)
                               TATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCT                                                               <  SRR4302535.2299328/94‑1 (MQ=60)
                                ATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTT                                                              >  SRR4302535.2182753/1‑94 (MQ=60)
                                  CAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTT                                                            >  SRR4302535.1133901/1‑94 (MQ=60)
                                  CAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTT                                                            <  SRR4302535.3885782/94‑1 (MQ=60)
                                   AGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTG                                                           <  SRR4302535.3299822/94‑1 (MQ=60)
                                   AGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTG                                                           >  SRR4302535.1215752/1‑94 (MQ=60)
                                   AGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTG                                                           >  SRR4302535.4499648/1‑94 (MQ=60)
                                    GCAAACTGCGACCAGCGATGTACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGG                                                          <  SRR4302535.1412416/94‑1 (MQ=60)
                                      AAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGTCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGT                                                        <  SRR4302535.1220361/94‑1 (MQ=60)
                                      AAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGT                                                        >  SRR4302535.4041445/1‑94 (MQ=60)
                                      AAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGT                                                        <  SRR4302535.2825398/94‑1 (MQ=60)
                                      AAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGT                                                        >  SRR4302535.848120/1‑94 (MQ=60)
                                         CTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGTCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATG                                                     <  SRR4302535.1762990/94‑1 (MQ=60)
                                         CTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATG                                                     <  SRR4302535.780892/94‑1 (MQ=60)
                                          TGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGC                                                    >  SRR4302535.819119/1‑94 (MQ=60)
                                           GCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCA                                                   <  SRR4302535.1816550/94‑1 (MQ=60)
                                           GCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCA                                                   <  SRR4302535.4704364/94‑1 (MQ=60)
                                             GACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAG                                                 <  SRR4302535.3019995/94‑1 (MQ=60)
                                             GACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAG                                                 <  SRR4302535.3069230/94‑1 (MQ=60)
                                             GACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAG                                                 <  SRR4302535.4652601/94‑1 (MQ=60)
                                                CAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTT                                              <  SRR4302535.2222034/94‑1 (MQ=60)
                                                 AGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTC                                             <  SRR4302535.2493321/94‑1 (MQ=60)
                                                   CGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGT                                           >  SRR4302535.3504832/1‑94 (MQ=60)
                                                   CGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGT                                           >  SRR4302535.3965125/1‑94 (MQ=60)
                                                    GATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACCGCTTTTGGGTATGCAAGGTTCGTG                                          <  SRR4302535.1234455/94‑1 (MQ=60)
                                                     ATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGC                                         >  SRR4302535.3031835/1‑94 (MQ=60)
                                                      TGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCT                                        <  SRR4302535.601974/94‑1 (MQ=60)
                                                          CATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATC                                    >  SRR4302535.531811/1‑94 (MQ=60)
                                                          CATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCATGCTCATC                                    >  SRR4302535.4204852/1‑94 (MQ=60)
                                                           ATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCA                                   >  SRR4302535.1756727/1‑94 (MQ=60)
                                                           ATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCA                                   >  SRR4302535.917146/1‑94 (MQ=60)
                                                           ATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTAGGTATGCAAGGTTCGTGCTCATCA                                   >  SRR4302535.1885682/1‑94 (MQ=60)
                                                            TTTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAG                                  <  SRR4302535.1815028/94‑1 (MQ=60)
                                                             TTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGG                                 <  SRR4302535.3106801/94‑1 (MQ=60)
                                                             TTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGG                                 <  SRR4302535.3808710/94‑1 (MQ=60)
                                                             TTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGG                                 <  SRR4302535.1351427/94‑1 (MQ=60)
                                                             TTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGG                                 <  SRR4302535.2843112/94‑1 (MQ=60)
                                                             TTTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGG                                 <  SRR4302535.784479/94‑1 (MQ=60)
                                                              TTCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGC                                <  SRR4302535.2957502/94‑1 (MQ=60)
                                                               TCTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCG                               <  SRR4302535.597090/94‑1 (MQ=60)
                                                                CTGTGCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGC                              >  SRR4302535.180802/1‑94 (MQ=60)
                                                                 TGTGCTCACTTTCGCGCCCAAAGTATGTCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCT                             >  SRR4302535.1503956/1‑94 (MQ=60)
                                                                    GCTCACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTT                          <  SRR4302535.2071479/94‑1 (MQ=60)
                                                                       CACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAAC                       <  SRR4302535.2193847/94‑1 (MQ=60)
                                                                       CACTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAAC                       <  SRR4302535.1463744/94‑1 (MQ=60)
                                                                         CTTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGG                     <  SRR4302535.3424699/94‑1 (MQ=60)
                                                                          TTTCGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGC                    <  SRR4302535.1497448/94‑1 (MQ=60)
                                                                             CGCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATT                 <  SRR4302535.3793311/94‑1 (MQ=60)
                                                                              GCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTC                <  SRR4302535.2315594/94‑1 (MQ=60)
                                                                              GCGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTC                >  SRR4302535.3306236/1‑94 (MQ=60)
                                                                               CGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCC               <  SRR4302535.4171761/94‑1 (MQ=60)
                                                                               CGCCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCC               >  SRR4302535.2869462/1‑94 (MQ=60)
                                                                                 CCCAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTGTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCAC             <  SRR4302535.3826277/94‑1 (MQ=60)
                                                                                   CAAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGC           >  SRR4302535.2913893/1‑94 (MQ=60)
                                                                                    AAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCG          >  SRR4302535.4220610/1‑94 (MQ=60)
                                                                                    AAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCG          >  SRR4302535.2101213/1‑94 (MQ=60)
                                                                                    AAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCG          >  SRR4302535.1853132/1‑94 (MQ=60)
                                                                                    AAAGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCG          <  SRR4302535.1224239/94‑1 (MQ=60)
                                                                                      AGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAG        >  SRR4302535.3950475/1‑94 (MQ=60)
                                                                                      AGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAG        >  SRR4302535.1348393/1‑94 (MQ=60)
                                                                                      AGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAG        <  SRR4302535.3704030/94‑1 (MQ=60)
                                                                                      AGTATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTACACGCGAG        >  SRR4302535.1967475/1‑94 (MQ=60)
                                                                                        TATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCC      <  SRR4302535.2592650/94‑1 (MQ=60)
                                                                                         ATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCA     <  SRR4302535.3794633/94‑1 (MQ=60)
                                                                                         ATGCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCA     >  SRR4302535.2479890/1‑94 (MQ=60)
                                                                                           GCCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCATC   >  SRR4302535.3509696/1‑94 (MQ=60)
                                                                                            CCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCATCT  <  SRR4302535.3576084/94‑1 (MQ=60)
                                                                                            CCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCATCT  <  SRR4302535.2412320/94‑1 (MQ=60)
                                                                                            CCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCATCT  >  SRR4302535.1546117/1‑94 (MQ=60)
                                                                                            CCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCATCT  <  SRR4302535.3921068/94‑1 (MQ=60)
                                                                                            CCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCATCT  <  SRR4302535.3967737/94‑1 (MQ=60)
                                                                                            |                                                                                             
TGCAAGGTTGCATCAGGTGTTAACCCGGTAATATCAGCAAACTGCGACCAGCGATGGACATTTTCTGTGCTCACTTTCGCGCCCAAAGTATGTCCTCCCATCACAGCAATCACGCAGCAACTGCTTTTGGGTATGCAAGGTTCGTGCTCATCAGGCGCTTTTAACGGCATTCCACGCGAGCCATCT  >  NC_000913/3012528‑3012713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑