Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,772	T→C	C898R (TGC→CGC)	b2878 →	putative oxidoreductase Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,772	0	T	C	100.0%	970.3 / NA	309	C898R (TGC→CGC)	b2878	putative oxidoreductase Fe‑S subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (171/138); total (171/138)

Only 100 of 338 total aligned reads displayed.

GCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCTGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAAC  >  NC_000913/3016682‑3016861

gCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTcgccgc                                                                                         >  1:3547349/1‑93 (MQ=255)

  ggAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCt                                                                                       <  1:4150399/93‑1 (MQ=255)

   gAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGCACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGACGCTCGCCGCCTc                                                                                      >  2:1048838/1‑93 (MQ=255)

   gAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTc                                                                                      <  1:38409/93‑1 (MQ=255)

    aaaTCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCg                                                                                     >  1:7404870/1‑93 (MQ=255)

     aaTCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGa                                                                                    >  1:7894550/1‑93 (MQ=255)

      aTCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGaa                                                                                   >  2:4155122/1‑93 (MQ=255)

      aTCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGaa                                                                                   <  1:4948737/93‑1 (MQ=255)

      aTCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGaa                                                                                   <  2:2542168/93‑1 (MQ=255)

       tCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAt                                                                                  >  1:7326003/1‑93 (MQ=255)

       tCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAt                                                                                  >  2:5484627/1‑93 (MQ=255)

       tCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAt                                                                                  >  2:4403958/1‑93 (MQ=255)

        cTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATg                                                                                 >  2:7365947/1‑93 (MQ=255)

        cTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATg                                                                                 >  1:1957572/1‑93 (MQ=255)

           tCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTaa                                                                              >  1:7086009/1‑93 (MQ=255)

           tCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTaa                                                                              <  1:4534320/93‑1 (MQ=255)

           tCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTaa                                                                              >  2:3076338/1‑93 (MQ=255)

           tCAACGTAAAGGCGATATCTCTACCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTaa                                                                              <  1:3056804/93‑1 (MQ=255)

              aCGTAAAGGCGATATCTCTATCACGCTGGTGCACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTa                                                                           >  2:3263924/1‑93 (MQ=255)

              aCGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTa                                                                           <  1:2696844/93‑1 (MQ=255)

               cGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTAc                                                                          <  1:5480366/93‑1 (MQ=255)

                 tAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGt                                                                        >  2:3127726/1‑93 (MQ=255)

                  aaaGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGtt                                                                       <  1:3687646/93‑1 (MQ=255)

                   aaGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGttt                                                                      <  1:7719844/93‑1 (MQ=255)

                    aGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTg                                                                     >  2:2115885/1‑93 (MQ=255)

                    aGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTg                                                                     >  2:450592/1‑93 (MQ=255)

                     ggCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTgc                                                                    >  1:3179559/1‑93 (MQ=255)

                     ggCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTgc                                                                    <  2:6332372/93‑1 (MQ=255)

                      gCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTgca                                                                   <  2:2958359/93‑1 (MQ=255)

                       cGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTgcag                                                                  >  2:2744611/1‑93 (MQ=255)

                        gATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCTGCTCGCCGCCTCGAATGTAACTACGTTTgcagc                                                                 >  1:7601298/1‑93 (MQ=255)

                        gATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTgcagc                                                                 >  1:7676328/1‑93 (MQ=255)

                        gATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTgcagc                                                                 >  1:1112401/1‑93 (MQ=255)

                         atatCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTgcagca                                                                >  2:895996/1‑93 (MQ=255)

                         atatCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTgcagca                                                                >  1:893552/1‑93 (MQ=255)

                          tatCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCaa                                                               >  2:6565899/1‑93 (MQ=255)

                          tatCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCaa                                                               >  2:3699630/1‑93 (MQ=255)

                          aatCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCaa                                                               <  2:7122508/92‑1 (MQ=255)

                           atCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAg                                                              >  2:3166774/1‑93 (MQ=255)

                            tctcTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAgt                                                             >  2:1800517/1‑93 (MQ=255)

                              tctATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAgtgt                                                           >  1:3005257/1‑93 (MQ=255)

                              tctATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAgtgg                                                           >  1:6475765/1‑92 (MQ=255)

                                tATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAgtgtgt                                                         >  2:821460/1‑93 (MQ=255)

                                 aTCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGtgtgtg                                                        <  2:7468457/93‑1 (MQ=255)

                                    aCGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGAt                                                     <  1:2156337/93‑1 (MQ=255)

                                    aCGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGAt                                                     <  2:3304193/93‑1 (MQ=255)

                                      gCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGt                                                   <  2:7479704/93‑1 (MQ=255)

                                       cTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTc                                                  >  1:4437337/1‑93 (MQ=255)

                                        tGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCt                                                 >  1:661334/1‑93 (MQ=255)

                                         ggTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTg                                                <  2:5578821/93‑1 (MQ=255)

                                           tGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGcc                                              >  1:5179930/1‑93 (MQ=255)

                                              aCAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGa                                           >  1:2766463/1‑93 (MQ=255)

                                               cAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGaa                                          <  2:4745336/93‑1 (MQ=255)

                                                aGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAAc                                         <  1:5104169/93‑1 (MQ=255)

                                                aGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAAc                                         >  2:1527067/1‑93 (MQ=255)

                                                  tGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAAccc                                       >  2:143542/1‑92 (MQ=255)

                                                  tGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAATGTGTGGATGTCTGCCCGAACcg                                       >  2:4295152/1‑93 (MQ=255)

                                                   taCGATCGTTACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACcgc                                      <  2:7296579/92‑1 (MQ=255)

                                                   gACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACcgc                                      <  2:5742530/93‑1 (MQ=255)

                                                       aTCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGCACCGCGCCa                                  >  2:7830532/1‑93 (MQ=255)

                                                       aTCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCa                                  >  2:27944/1‑93 (MQ=255)

                                                       aTCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCa                                  >  2:1937995/1‑93 (MQ=255)

                                                        tCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCaa                                 >  2:7049315/1‑93 (MQ=255)

                                                        tCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCaa                                 >  2:5427784/1‑93 (MQ=255)

                                                        tCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCaa                                 <  1:5969427/93‑1 (MQ=255)

                                                          gTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACg                               >  1:3992414/1‑93 (MQ=255)

                                                          gTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACg                               >  2:2767661/1‑93 (MQ=255)

                                                           tGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGt                              >  1:6304097/1‑93 (MQ=255)

                                                            gACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGtc                             <  1:4839265/93‑1 (MQ=255)

                                                             aCGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGtct                            >  2:3001606/1‑93 (MQ=255)

                                                             aCGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGtct                            >  1:3296130/1‑93 (MQ=255)

                                                              cgcgTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGtctc                           >  2:4842951/1‑93 (MQ=255)

                                                               gcgTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTcc                          >  2:6207688/1‑93 (MQ=255)

                                                                cgTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCa                         <  2:2383783/93‑1 (MQ=255)

                                                                  tttGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGCACCGCGCCAACGTCTCCAtt                       >  1:1026792/1‑93 (MQ=255)

                                                                  tttGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCAtt                       >  1:2663969/1‑93 (MQ=255)

                                                                    tGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGc                     <  2:6260379/93‑1 (MQ=255)

                                                                     gTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCg                    <  1:1639342/93‑1 (MQ=255)

                                                                      tCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCgg                   <  2:3615335/93‑1 (MQ=255)

                                                                         cccAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTcc                >  2:5031528/1‑93 (MQ=255)

                                                                          ccAGGAAGCCGCTCGCCGCCTCTAATGTAACTACGTTTGCTTCAAGTGTGTGGATGTCGGCCTTTTCCGCGCCAACGTCTTCATTTCGGTccc               >  2:7005960/1‑93 (MQ=255)

                                                                           cAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCa              >  1:1556540/1‑93 (MQ=255)

                                                                           cAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCa              >  2:7703186/1‑93 (MQ=255)

                                                                           cAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCa              <  2:5852474/93‑1 (MQ=255)

                                                                           cAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCa              <  2:7545268/93‑1 (MQ=255)

                                                                           cAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGGCTCCATTGCGGTCCCa              >  1:7035281/1‑93 (MQ=255)

                                                                            aGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAg             <  2:4946833/93‑1 (MQ=255)

                                                                            aGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAg             <  2:3995173/93‑1 (MQ=255)

                                                                            aGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAg             >  2:1672962/1‑93 (MQ=255)

                                                                             ggAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAgg            >  1:5755991/1‑93 (MQ=255)

                                                                             ggAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAgg            >  2:3243468/1‑93 (MQ=255)

                                                                             ggAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAgg            >  1:3646681/1‑93 (MQ=255)

                                                                              gAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGc           <  1:132108/93‑1 (MQ=255)

                                                                              gAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGc           <  1:2441240/93‑1 (MQ=255)

                                                                                 gCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTc        >  2:6543961/1‑93 (MQ=255)

                                                                                 gCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGCGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTc        >  1:4619168/1‑93 (MQ=255)

                                                                                 cccGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTc        <  1:4285801/92‑1 (MQ=255)

                                                                                   cgctcgccGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCa      <  2:4057715/93‑1 (MQ=255)

                                                                                      tcgccGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGaa   >  2:7205268/1‑93 (MQ=255)

                                                                                      tcgccGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCACTGCGGTCCCAGGCTTCCAGaa   >  2:5759670/1‑93 (MQ=255)

GCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCTGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAAC  >  NC_000913/3016682‑3016861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCAGCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCTGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCGTT  >  NC_000913/3016679‑3016865

CCAGCGGAAATCTATCAACGTAAAGGCGATAGCTCTATCCCGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCC                                                                                               <  SRR4302543.2216461/94‑1 (MQ=60)

  AGCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGC                                                                                             >  SRR4302543.3578836/1‑94 (MQ=60)

   GCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCC                                                                                            <  SRR4302543.4428403/94‑1 (MQ=60)

     GGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCGGCTCGGCGCCTC                                                                                          >  SRR4302543.3837632/1‑94 (MQ=60)

     GGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTC                                                                                          <  SRR4302543.4187919/94‑1 (MQ=60)

     GGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTC                                                                                          <  SRR4302543.1105482/94‑1 (MQ=60)

      GAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCG                                                                                         >  SRR4302543.4619952/1‑94 (MQ=60)

      GAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCG                                                                                         <  SRR4302543.38764/94‑1 (MQ=60)

      GAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCG                                                                                         >  SRR4302543.7475942/1‑94 (MQ=60)

      GAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCG                                                                                         <  SRR4302543.1642179/94‑1 (MQ=60)

       AAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGA                                                                                        >  SRR4302543.7968785/1‑94 (MQ=60)

        AATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAA                                                                                       >  SRR4302543.3550939/1‑94 (MQ=60)

        AATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAA                                                                                       >  SRR4302543.7571869/1‑94 (MQ=60)

         ATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAT                                                                                      >  SRR4302543.5152659/1‑94 (MQ=60)

         ATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAT                                                                                      <  SRR4302543.4993799/94‑1 (MQ=60)

         ATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAT                                                                                      >  SRR4302543.3794949/1‑94 (MQ=60)

         ATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAT                                                                                      >  SRR4302543.3085245/1‑94 (MQ=60)

         ATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAT                                                                                      >  SRR4302543.7396535/1‑94 (MQ=60)

         ATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAT                                                                                      >  SRR4302543.2841924/1‑94 (MQ=60)

         ATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAAT                                                                                      >  SRR4302543.2009943/1‑94 (MQ=60)

          TCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATG                                                                                     >  SRR4302543.974365/1‑94 (MQ=60)

          TCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATG                                                                                     >  SRR4302543.6513037/1‑94 (MQ=60)

          TCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATG                                                                                     <  SRR4302543.6436000/94‑1 (MQ=60)

          TCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATG                                                                                     <  SRR4302543.4467799/94‑1 (MQ=60)

          TCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATG                                                                                     >  SRR4302543.1975132/1‑94 (MQ=60)

            TATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTA                                                                                   >  SRR4302543.438227/1‑94 (MQ=60)

            TATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTA                                                                                   <  SRR4302543.6551482/94‑1 (MQ=60)

             ATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAA                                                                                  >  SRR4302543.389167/1‑94 (MQ=60)

             ATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAA                                                                                  >  SRR4302543.872027/1‑94 (MQ=60)

             ATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAA                                                                                  <  SRR4302543.5242263/94‑1 (MQ=60)

             ATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAA                                                                                  >  SRR4302543.7154823/1‑94 (MQ=60)

             ATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAA                                                                                  >  SRR4302543.3160867/1‑94 (MQ=60)

             ATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAA                                                                                  <  SRR4302543.7423964/94‑1 (MQ=60)

              TCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAAC                                                                                 <  SRR4302543.4575597/94‑1 (MQ=60)

              TCAACGTAAAGGCGATATCTCTACCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAAC                                                                                 <  SRR4302543.3084321/94‑1 (MQ=60)

               CAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACT                                                                                <  SRR4302543.3587572/94‑1 (MQ=60)

                 ACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTAC                                                                              >  SRR4302543.7729426/1‑94 (MQ=60)

                 ACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTAC                                                                              <  SRR4302543.2721296/94‑1 (MQ=60)

                 ACGTAAAGGCGATACCTCTATCACGTTGGTGAACAGTGACTATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTAC                                                                              <  SRR4302543.2721295/94‑1 (MQ=60)

                  CGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGTATGTAACTACG                                                                             >  SRR4302543.3322437/1‑94 (MQ=60)

                  CGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACG                                                                             >  SRR4302543.3669353/1‑94 (MQ=60)

                  CGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACG                                                                             <  SRR4302543.5530036/94‑1 (MQ=60)

                   GTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGT                                                                            >  SRR4302543.3251900/1‑94 (MQ=60)

                    TAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTT                                                                           >  SRR4302543.2588332/1‑94 (MQ=60)

                     AAAGGCGATATCTCTATCTCGCTGGTGAACAGTGACGATCGTGCCGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTT                                                                          <  SRR4302543.6815689/94‑1 (MQ=60)

                     AAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTT                                                                          <  SRR4302543.4709793/94‑1 (MQ=60)

                     AAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTT                                                                          <  SRR4302543.3720054/94‑1 (MQ=60)

                     AAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTT                                                                          <  SRR4302543.7243767/94‑1 (MQ=60)

                     AAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTT                                                                          <  SRR4302543.2719925/94‑1 (MQ=60)

                      AAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTG                                                                         <  SRR4302543.7194328/94‑1 (MQ=60)

                      AAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTG                                                                         <  SRR4302543.7792846/94‑1 (MQ=60)

                      AAGGCGATAACTCTATCACGGCGGTGAACAGTGACGATCGTGAGGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTG                                                                         <  SRR4302543.6872392/94‑1 (MQ=60)

                       AGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGC                                                                        >  SRR4302543.3208223/1‑94 (MQ=60)

                        GGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCAAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCA                                                                       >  SRR4302543.2779540/1‑94 (MQ=60)

                         GCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAG                                                                      <  SRR4302543.4866147/94‑1 (MQ=60)

                         GCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAG                                                                      >  SRR4302543.4215283/1‑94 (MQ=60)

                          CGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCTGCTCGCCGCCTCGAATGTAACTACGTTTGCAGC                                                                     >  SRR4302543.7673492/1‑94 (MQ=60)

                          CGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGC                                                                     >  SRR4302543.1122454/1‑94 (MQ=60)

                          CGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGC                                                                     >  SRR4302543.7749049/1‑94 (MQ=60)

                           GATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCA                                                                    >  SRR4302543.901445/1‑94 (MQ=60)

                           GATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCA                                                                    >  SRR4302543.5086041/1‑94 (MQ=60)

                            ATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAA                                                                   >  SRR4302543.3080187/1‑94 (MQ=60)

                             TCTCTCTCTCACGCTGGTGTACAGTGACGATCGTGACGCATTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGcctgtccctccgtttgccgcaag                                                                  <  SRR4302543.6081281/94‑24 (MQ=60)

                             TATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAG                                                                  <  SRR4302543.4009361/94‑1 (MQ=60)

                              ATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGT                                                                 >  SRR4302543.5102607/1‑94 (MQ=60)

                              ATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGT                                                                 >  SRR4302543.7758133/1‑94 (MQ=60)

                                CTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGT                                                               <  SRR4302543.926108/94‑1 (MQ=60)

                                CTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGT                                                               >  SRR4302543.3032489/1‑94 (MQ=60)

                                CTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGG                                                               >  SRR4302543.6536778/1‑94 (MQ=60)

                                 TCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTG                                                              <  SRR4302543.448947/94‑1 (MQ=60)

                                  CTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGG                                                             >  SRR4302543.5981070/1‑94 (MQ=60)

                                   ttgctcgctggtgaacacgtACGATCGTGACGCGTTTGGCGCCCAGGAAGCCGCTCGCCGCCTGGAATGTACCTACGTTTGCAGCAAGTATGTG                                                            <  SRR4302543.1579029/74‑1 (MQ=60)

                                   TATCACGCTGGTGAACAGTGACGAGCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTG                                                            <  SRR4302543.2888804/94‑1 (MQ=60)

                                    ATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGG                                                           <  SRR4302543.7114099/94‑1 (MQ=60)

                                      CACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGagtgaggag                                                         >  SRR4302543.6037007/1‑85 (MQ=60)

                                      CACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGAT                                                         <  SRR4302543.664333/94‑1 (MQ=60)

                                      CACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGAT                                                         <  SRR4302543.6174471/94‑1 (MQ=60)

                                       ACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCTCCCAGGAAGCCGCTCGCCTCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATG                                                        <  SRR4302543.110204/94‑1 (MQ=60)

                                       ACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATG                                                        <  SRR4302543.2175649/94‑1 (MQ=60)

                                         GCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTC                                                      >  SRR4302543.4477525/1‑94 (MQ=60)

                                          CTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCT                                                     >  SRR4302543.667410/1‑94 (MQ=60)

                                          CTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCT                                                     <  SRR4302543.7016364/94‑1 (MQ=60)

                                          CTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCT                                                     >  SRR4302543.3688067/1‑94 (MQ=60)

                                            GGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGC                                                   >  SRR4302543.3451536/1‑94 (MQ=60)

                                             GTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCC                                                  >  SRR4302543.5226685/1‑94 (MQ=60)

                                              TGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCC                                                 <  SRR4302543.7841632/94‑1 (MQ=60)

                                                AACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGA                                               >  SRR4302543.2791641/1‑94 (MQ=60)

                                                  CAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAAC                                             >  SRR4302543.1925274/1‑94 (MQ=60)

                                                   AGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACC                                            <  SRR4302543.3655079/94‑1 (MQ=60)

                                                   AGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACC                                            <  SRR4302543.5150365/94‑1 (MQ=60)

                                                   AGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACC                                            >  SRR4302543.3312977/1‑94 (MQ=60)

                                                   AGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACC                                            >  SRR4302543.1269181/1‑94 (MQ=60)

                                                   AGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACC                                            >  SRR4302543.11232/1‑94 (MQ=60)

                                                    GTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCG                                           >  SRR4302543.460295/1‑94 (MQ=60)

                                                    GTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCG                                           >  SRR4302543.460294/1‑94 (MQ=60)

                                                    GTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGGAACTACGGTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCG                                           >  SRR4302543.7111253/1‑94 (MQ=60)

                                                    GTGACGATCGTGACGCGTTTGTCCGCCAGGAAGCCGCTCACCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCG                                           <  SRR4302543.371850/94‑1 (MQ=60)

                                                     TGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGC                                          >  SRR4302543.1929546/1‑94 (MQ=60)

                                                     TGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGC                                          <  SRR4302543.1606693/94‑1 (MQ=60)

                                                     TGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGC                                          >  SRR4302543.1413649/1‑94 (MQ=60)

                                                       ACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGC                                        >  SRR4302543.7458908/1‑94 (MQ=60)

                                                        CGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCC                                       >  SRR4302543.7642160/1‑94 (MQ=60)

                                                         GATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCA                                      >  SRR4302543.389754/1‑94 (MQ=60)

                                                          ATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAA                                     >  SRR4302543.8010425/1‑94 (MQ=60)

                                                          ATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAA                                     >  SRR4302543.7048454/1‑94 (MQ=60)

                                                           TCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAAC                                    <  SRR4302543.6024088/94‑1 (MQ=60)

                                                           TCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAAC                                    <  SRR4302543.3072982/94‑1 (MQ=60)

                                                            CGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACG                                   <  SRR4302543.1415959/94‑1 (MQ=60)

                                                            CGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACG                                   <  SRR4302543.5075278/94‑1 (MQ=60)

                                                            CGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACG                                   <  SRR4302543.7277670/94‑1 (MQ=60)

                                                            CGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACG                                   >  SRR4302543.6300947/1‑94 (MQ=60)

                                                            CGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACG                                   >  SRR4302543.4027937/1‑94 (MQ=60)

                                                            CGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACG                                   <  SRR4302543.886351/94‑1 (MQ=60)

                                                             GTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGT                                  >  SRR4302543.6363402/1‑94 (MQ=60)

                                                               GACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCT                                <  SRR4302543.4883397/94‑1 (MQ=60)

                                                               GACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCT                                >  SRR4302543.3325865/1‑94 (MQ=60)

                                                                ACGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTC                               <  SRR4302543.4161469/94‑1 (MQ=60)

                                                                 CGCGTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCC                              <  SRR4302543.5878189/94‑1 (MQ=60)

                                                                    GTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGCACCGCGCCAACGTCTCCATT                           >  SRR4302543.1035891/1‑94 (MQ=60)

                                                                    GTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATT                           <  SRR4302543.1436888/94‑1 (MQ=60)

                                                                    GTTTGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATT                           >  SRR4302543.2688123/1‑94 (MQ=60)

                                                                       TGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCG                        <  SRR4302543.7685933/94‑1 (MQ=60)

                                                                       TGTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCG                        <  SRR4302543.2945769/94‑1 (MQ=60)

                                                                        GTCGCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGG                       <  SRR4302543.1653963/94‑1 (MQ=60)

                                                                           GCCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCC                    <  SRR4302543.7892110/94‑1 (MQ=60)

                                                                            CCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCC                   >  SRR4302543.5581464/1‑94 (MQ=60)

                                                                            CCCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGCCTGCCCACACCGCGCAAACGTCTCCATTGCGctcac                   >  SRR4302543.2761915/1‑89 (MQ=60)

                                                                             CCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCA                  >  SRR4302543.1570452/1‑94 (MQ=60)

                                                                             CCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCA                  <  SRR4302543.4953497/94‑1 (MQ=60)

                                                                             CCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCA                  >  SRR4302543.5207260/1‑94 (MQ=60)

                                                                             CCAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGGCTCCATTGCGGTCCCA                  >  SRR4302543.7103016/1‑94 (MQ=60)

                                                                              CAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGTGCCAACGTCTCCATTGCGGTCCCAG                 <  SRR4302543.4078254/94‑1 (MQ=60)

                                                                              CAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAG                 >  SRR4302543.2003094/1‑94 (MQ=60)

                                                                              CAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAG                 >  SRR4302543.4971887/1‑94 (MQ=60)

                                                                              CAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAG                 >  SRR4302543.1992626/1‑94 (MQ=60)

                                                                              CAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAG                 <  SRR4302543.232549/94‑1 (MQ=60)

                                                                              CAGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAG                 >  SRR4302543.248952/1‑94 (MQ=60)

                                                                               AGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGG                >  SRR4302543.5808443/1‑94 (MQ=60)

                                                                               AGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGG                <  SRR4302543.2333186/94‑1 (MQ=60)

                                                                               AGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGG                >  SRR4302543.7783963/1‑94 (MQ=60)

                                                                               AGGAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGG                >  SRR4302543.3678743/1‑94 (MQ=60)

                                                                                 GAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCT              <  SRR4302543.133285/94‑1 (MQ=60)

                                                                                 GAAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCT              <  SRR4302543.2463249/94‑1 (MQ=60)

                                                                                  AAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTT             <  SRR4302543.4548380/94‑1 (MQ=60)

                                                                                  AAGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTT             <  SRR4302543.5334737/94‑1 (MQ=60)

                                                                                   AGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTC            <  SRR4302543.5630714/94‑1 (MQ=60)

                                                                                   AGCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGCGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTC            >  SRR4302543.4661134/1‑94 (MQ=60)

                                                                                    GCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCC           <  SRR4302543.4324751/94‑1 (MQ=60)

                                                                                    CCCGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCC           <  SRR4302543.4324752/94‑1 (MQ=60)

                                                                                      CGCTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAG         >  SRR4302543.5358014/1‑94 (MQ=60)

                                                                                        CTCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAA       >  SRR4302543.2686540/1‑94 (MQ=60)

                                                                                         TCGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAAC      <  SRR4302543.7704439/94‑1 (MQ=60)

                                                                                          CGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACC     >  SRR4302543.3570195/1‑94 (MQ=60)

                                                                                          CGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACC     >  SRR4302543.513665/1‑94 (MQ=60)

                                                                                          CGCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACC     >  SRR4302543.7426550/1‑94 (MQ=60)

                                                                                           GCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCG    <  SRR4302543.4955602/94‑1 (MQ=60)

                                                                                           GCCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCG    >  SRR4302543.4349282/1‑94 (MQ=60)

                                                                                            CCGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCGT   <  SRR4302543.6871179/94‑1 (MQ=60)

                                                                                             CGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCGTT  >  SRR4302543.1908510/1‑94 (MQ=60)

                                                                                             CGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCGTT  >  SRR4302543.34333/1‑94 (MQ=60)

                                                                                             CGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCGTT  >  SRR4302543.7380541/1‑94 (MQ=60)

                                                                                             CGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCGTT  >  SRR4302543.1297843/1‑94 (MQ=60)

                                                                                             CGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCGTT  >  SRR4302543.2272761/1‑94 (MQ=60)

                                                                                             CGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCGTT  >  SRR4302543.7070142/1‑94 (MQ=60)

CCAGCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTGTCGCCCAGGAAGCCGCTCGCTGCCTCGAATGTAACTACGTTTGCAGCAAGTGTGTGGATGTCTGCCCGAACCGCGCCAACGTCTCCATTGCGGTCCCAGGCTTCCAGAACCGTT  >  NC_000913/3016679‑3016865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`