Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,018,487	C→T	R436C (CGT→TGT)	b2879 →	putative proteoglycan

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,018,487	0	C	T	100.0%	730.0 / NA	202	R436C (CGT→TGT)	b2879	putative proteoglycan
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (96/106); total (96/106)

Only 100 of 211 total aligned reads displayed.

AATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTT  >  NC_000913/3018397‑3018576

 aTGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTc                                                                                        >  1:3719280/1‑93 (MQ=255)

  tGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCg                                                                                       <  1:4628504/93‑1 (MQ=255)

    gtgtGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCgga                                                                                     >  2:2971518/1‑93 (MQ=255)

    gtgtGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCgga                                                                                     >  2:1937166/1‑93 (MQ=255)

     tgtgATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCggat                                                                                    <  2:543786/93‑1 (MQ=255)

     tgtgATGGACTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCggat                                                                                    >  2:4553607/1‑93 (MQ=255)

     tgtgAGGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCggat                                                                                    <  2:1715220/93‑1 (MQ=255)

          tGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGgatggatg                                                                               <  2:4395815/93‑1 (MQ=255)

             tCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATgcgc                                                                            <  2:3458509/93‑1 (MQ=255)

                aTGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTgg                                                                         >  1:653224/1‑93 (MQ=255)

                 tGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTggt                                                                        >  2:3865598/1‑92 (MQ=255)

                 tGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGc                                                                        >  2:2063364/1‑93 (MQ=255)

                  gAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCa                                                                       >  2:2484019/1‑93 (MQ=255)

                      aCCGTCAGTTTAACTTCTATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATaaa                                                                   <  2:424325/93‑1 (MQ=255)

                      aCCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATaaa                                                                   <  2:3314015/93‑1 (MQ=255)

                      aCCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATaaa                                                                   <  1:1932216/93‑1 (MQ=255)

                        cGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATg                                                                 <  2:4317910/93‑1 (MQ=255)

                         gTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGa                                                                >  1:3926098/1‑93 (MQ=255)

                          tCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGAc                                                               >  1:3414180/1‑93 (MQ=255)

                            aGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAg                                                             <  1:303603/93‑1 (MQ=255)

                            aGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGGATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAg                                                             >  1:4681860/1‑93 (MQ=255)

                             gTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGa                                                            <  2:4173579/93‑1 (MQ=255)

                             gTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGa                                                            <  1:3842087/93‑1 (MQ=255)

                             gTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGa                                                            >  2:2141557/1‑93 (MQ=255)

                              tttAACTTGGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGAt                                                           <  1:226247/93‑1 (MQ=255)

                              tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGAt                                                           >  2:4663857/1‑93 (MQ=255)

                              tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGAt                                                           <  1:2856348/93‑1 (MQ=255)

                              tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGAt                                                           >  1:1712022/1‑93 (MQ=255)

                              tttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGAt                                                           <  2:3283025/93‑1 (MQ=255)

                              tttAACTTCGATTGCGATTCCATTAATGAACAAGCCAGAAAAGACGCGGCCCGTATGTGGGGTGGGTTGAATGCGCTGGCCTAAGTGACAgtt                                                           >  2:4518820/1‑91 (MQ=255)

                               ttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATg                                                          <  2:23077/93‑1 (MQ=255)

                               ttAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATg                                                          <  2:2599000/93‑1 (MQ=255)

                                  aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGTGCTGGCATAAATGACAGATGccc                                                       >  1:1826886/1‑93 (MQ=255)

                                  aCTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGccc                                                       <  1:755974/93‑1 (MQ=255)

                                    ttCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCctc                                                     >  1:2437776/1‑93 (MQ=255)

                                    ttCGATTGCAATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCctc                                                     <  2:1777284/93‑1 (MQ=255)

                                       gATTGCTATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTc                                                  <  1:4087956/93‑1 (MQ=255)

                                       gATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGCTGCCCTCTTc                                                  >  1:4467029/1‑93 (MQ=255)

                                       gATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTc                                                  >  2:1282508/1‑93 (MQ=255)

                                        aTTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTcc                                                 >  1:3910572/1‑93 (MQ=255)

                                        aTTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTcc                                                 >  1:4512761/1‑93 (MQ=255)

                                        aTTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTcc                                                 >  2:3625313/1‑93 (MQ=255)

                                         ttGGGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGGCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTccc                                                <  1:2919769/93‑1 (MQ=255)

                                         ttGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTccc                                                >  1:1631238/1‑93 (MQ=255)

                                              aTTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAgg                                           >  1:910985/1‑93 (MQ=255)

                                                tCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGa                                         <  2:1828113/93‑1 (MQ=255)

                                                 ccATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGaa                                        >  2:1276971/1‑93 (MQ=255)

                                                 ccATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGaa                                        >  1:1996135/1‑93 (MQ=255)

                                                  cATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAag                                       >  1:1005662/1‑93 (MQ=255)

                                                  cATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAag                                       >  2:752875/1‑93 (MQ=255)

                                                   aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAaga                                      <  2:352998/93‑1 (MQ=255)

                                                   aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAaga                                      <  2:2682213/93‑1 (MQ=255)

                                                   aTTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAaga                                      >  1:2896746/1‑93 (MQ=255)

                                                    tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                     >  1:2743268/1‑93 (MQ=255)

                                                    tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                     <  1:1592763/93‑1 (MQ=255)

                                                    tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                     <  1:1302110/93‑1 (MQ=255)

                                                    tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                     >  1:3116337/1‑93 (MQ=255)

                                                    tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                     <  1:3361161/93‑1 (MQ=255)

                                                    tttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAagag                                     >  1:3666664/1‑93 (MQ=255)

                                                     ttATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAgg                                    >  2:3791769/1‑93 (MQ=255)

                                                       gtccacaAGCAAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCGGGCATAAATGACAGATGCCTTCTTCCCGCAGGGAAGAGGGt                                  <  2:3393787/90‑1 (MQ=255)

                                                        tGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGtt                                 <  1:2776663/93‑1 (MQ=255)

                                                           acaAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTaaa                              <  2:2821737/93‑1 (MQ=255)

                                                             aaGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATa                            <  2:2899732/93‑1 (MQ=255)

                                                             aaGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGGTAAATa                            >  2:2645535/1‑93 (MQ=255)

                                                              aGCCAGAAAAGCCGCTGCCAGTATGTCGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATaa                           <  1:255378/93‑1 (MQ=255)

                                                                 cAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGa                        <  2:299797/93‑1 (MQ=255)

                                                                  aGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaa                       <  2:4282711/93‑1 (MQ=255)

                                                                  aGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaa                       >  2:1124582/1‑93 (MQ=255)

                                                                  aGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaa                       >  1:1653793/1‑93 (MQ=255)

                                                                   gAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGaaa                      >  1:3612711/1‑93 (MQ=255)

                                                                     aaaGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaaga                    >  1:435068/1‑93 (MQ=255)

                                                                      aaGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaa                   >  2:5129/1‑93 (MQ=255)

                                                                      aaGCCGCTGCCAGCATGTGGTGTGGGGTGGGTGTGCTGGGGTAAATGACAGATGCCCTCTTCCCGCCGGGACGAGGGGTAAATAAagaaagaa                   >  2:3983200/1‑93 (MQ=255)

                                                                       aGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaag                  >  1:1731745/1‑93 (MQ=255)

                                                                       aGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAaagaag                  <  1:1415656/93‑1 (MQ=255)

                                                                        gCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAagaaga                 <  2:4072968/93‑1 (MQ=255)

                                                                        gCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAagaaga                 <  1:827367/93‑1 (MQ=255)

                                                                        gCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAagaaga                 <  2:1815255/93‑1 (MQ=255)

                                                                        gCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAagaaga                 >  2:539649/1‑93 (MQ=255)

                                                                         ccGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGGGGGTTAAATAAGGAAAGAAgat                <  1:813090/93‑1 (MQ=255)

                                                                         ccGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATCCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgat                <  1:2510103/93‑1 (MQ=255)

                                                                           gCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatga              <  2:900383/93‑1 (MQ=255)

                                                                           gCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatga              >  1:4130183/1‑93 (MQ=255)

                                                                            cTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAgatgat             <  2:4662085/93‑1 (MQ=255)

                                                                             tGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt            >  1:2142349/1‑93 (MQ=255)

                                                                             tGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt            <  2:716227/93‑1 (MQ=255)

                                                                             tGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGAtt            >  1:887912/1‑93 (MQ=255)

                                                                               ccAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGa          >  1:1267187/1‑93 (MQ=255)

                                                                                cAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGaa         <  1:3321128/93‑1 (MQ=255)

                                                                                 aGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAAc        <  1:546899/93‑1 (MQ=255)

                                                                                 aGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAAc        >  1:4116063/1‑93 (MQ=255)

                                                                                   tATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACaa      <  1:3410912/93‑1 (MQ=255)

                                                                                   tATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACaa      <  2:1223772/93‑1 (MQ=255)

                                                                                    aTGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAt     <  1:4592642/93‑1 (MQ=255)

                                                                                     tgtgGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAtt    <  2:1750659/93‑1 (MQ=255)

                                                                                      gtggtgTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttt   <  1:512650/93‑1 (MQ=255)

                                                                                      gtggtgTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAttt   <  2:2738412/93‑1 (MQ=255)

                                                                                       tgGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAtttt  >  2:4367725/1‑93 (MQ=255)

                                                                                       tgGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAtttt  >  2:800036/1‑93 (MQ=255)

AATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTT  >  NC_000913/3018397‑3018576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAG  >  NC_000913/3018394‑3018580

GTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGT                                                                                               >  SRR4302535.3222678/1‑94 (MQ=60)

GTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGT                                                                                               <  SRR4302535.1541865/94‑1 (MQ=60)

 TCAATGGTGTGATGGTCTATGAAGCCCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACACGCCAGAAAAGCCGCTGCCAGTATGTGGTG                                                                                              <  SRR4302535.193919/94‑1 (MQ=60)

 TCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTG                                                                                              >  SRR4302535.4192338/1‑94 (MQ=60)

  CAATGGTGTGATGGTCTATGAAGCCCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGT                                                                                             <  SRR4302535.4035878/94‑1 (MQ=60)

   AATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTC                                                                                            >  SRR4302535.3745045/1‑94 (MQ=60)

   AATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTC                                                                                            <  SRR4302535.1655518/94‑1 (MQ=60)

    ATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCG                                                                                           <  SRR4302535.2827878/94‑1 (MQ=60)

     TGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGG                                                                                          <  SRR4302535.4659918/94‑1 (MQ=60)

           GATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGAT                                                                                    <  SRR4302535.3686506/94‑1 (MQ=60)

           GATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGAT                                                                                    >  SRR4302535.1798053/1‑94 (MQ=60)

           GATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGAT                                                                                    <  SRR4302535.2765462/94‑1 (MQ=60)

             TGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGC                                                                                  <  SRR4302535.4266942/94‑1 (MQ=60)

             TGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGC                                                                                  <  SRR4302535.3534130/94‑1 (MQ=60)

             TGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGC                                                                                  >  SRR4302535.2143718/1‑94 (MQ=60)

             TGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGC                                                                                  <  SRR4302535.1973567/94‑1 (MQ=60)

             TGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCAATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGG                                                                                  >  SRR4302535.2335381/1‑94 (MQ=60)

                TCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCT                                                                               >  SRR4302535.2845/1‑94 (MQ=60)

                  TATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGG                                                                             >  SRR4302535.657783/1‑94 (MQ=60)

                  TATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGG                                                                             >  SRR4302535.4108559/1‑94 (MQ=60)

                   ATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGC                                                                            >  SRR4302535.1622554/1‑94 (MQ=60)

                      AAGAGCGTCAGTGTAACTGGGATTGCGATACCATTTATCCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATA                                                                         <  SRR4302535.71008/94‑1 (MQ=60)

                       AGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAA                                                                        >  SRR4302535.903928/1‑94 (MQ=60)

                        GACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAA                                                                       >  SRR4302535.2915399/1‑94 (MQ=60)

                        GACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAA                                                                       >  SRR4302535.1153456/1‑94 (MQ=60)

                         ACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAAT                                                                      <  SRR4302535.1946138/94‑1 (MQ=60)

                          CCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATG                                                                     <  SRR4302535.3376701/94‑1 (MQ=60)

                           CGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGA                                                                    >  SRR4302535.3952922/1‑94 (MQ=60)

                           CGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGA                                                                    <  SRR4302535.1558122/94‑1 (MQ=60)

                            GTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGAC                                                                   >  SRR4302535.3437933/1‑94 (MQ=60)

                              CAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAG                                                                 >  SRR4302535.1705309/1‑94 (MQ=60)

                              CAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGGATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAG                                                                 >  SRR4302535.4713502/1‑94 (MQ=60)

                               AGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGA                                                                <  SRR4302535.305711/94‑1 (MQ=60)

                               AGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGA                                                                >  SRR4302535.2412582/1‑94 (MQ=60)

                                GTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGAT                                                               >  SRR4302535.1724401/1‑94 (MQ=60)

                                GTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGAT                                                               <  SRR4302535.3868556/94‑1 (MQ=60)

                                GTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGAT                                                               <  SRR4302535.4124521/94‑1 (MQ=60)

                                GTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGAT                                                               <  SRR4302535.3435837/94‑1 (MQ=60)

                                 TTTAACTTGGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATG                                                              <  SRR4302535.227800/94‑1 (MQ=60)

                                 TTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATG                                                              <  SRR4302535.2876588/94‑1 (MQ=60)

                                    AACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGTGCTGGCATAAATGACAGATGCCC                                                           >  SRR4302535.1840075/1‑94 (MQ=60)

                                     ACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCT                                                          <  SRR4302535.761298/94‑1 (MQ=60)

                                      CTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTC                                                         >  SRR4302535.2455336/1‑94 (MQ=60)

                                         CGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGCTGCCCTCTTC                                                      >  SRR4302535.4497677/1‑94 (MQ=60)

                                          GATTGCTATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCC                                                     <  SRR4302535.4115929/94‑1 (MQ=60)

                                          GATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCC                                                     >  SRR4302535.3937348/1‑94 (MQ=60)

                                          GATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCC                                                     >  SRR4302535.4543708/1‑94 (MQ=60)

                                           ATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCC                                                    >  SRR4302535.1643020/1‑94 (MQ=60)

                                            TTGGGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGGCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCG                                                   <  SRR4302535.2940370/94‑1 (MQ=60)

                                               gcattctattgacgccCAAGCCAGAAAAGCCGCTGCGAGTATGTGGTGTCGGAGGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAG                                                <  SRR4302535.1882649/78‑1 (MQ=60)

                                                GATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGG                                               <  SRR4302535.1295424/94‑1 (MQ=60)

                                                GATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGG                                               >  SRR4302535.917462/1‑94 (MQ=60)

                                                AATTCAATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGG                                               <  SRR4302535.724800/94‑1 (MQ=60)

                                                 ATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGG                                              >  SRR4302535.4665750/1‑94 (MQ=60)

                                                   TCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAA                                            >  SRR4302535.2010507/1‑94 (MQ=60)

                                                    CCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAG                                           >  SRR4302535.1012809/1‑94 (MQ=60)

                                                     CATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGG                                          >  SRR4302535.2959966/1‑94 (MQ=60)

                                                     CATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGA                                          >  SRR4302535.2917231/1‑94 (MQ=60)

                                                     CATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGA                                          >  SRR4302535.4717441/1‑94 (MQ=60)

                                                     CATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGA                                          >  SRR4302535.1854750/1‑94 (MQ=60)

                                                      ATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAG                                         >  SRR4302535.3138031/1‑94 (MQ=60)

                                                      ATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAG                                         >  SRR4302535.2762821/1‑94 (MQ=60)

                                                      ATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAG                                         >  SRR4302535.3692053/1‑94 (MQ=60)

                                                       TTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGG                                        <  SRR4302535.4163200/94‑1 (MQ=60)

                                                       TTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGG                                        <  SRR4302535.3384554/94‑1 (MQ=60)

                                                       TTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGG                                        <  SRR4302535.1311410/94‑1 (MQ=60)

                                                       TTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGG                                        <  SRR4302535.1604258/94‑1 (MQ=60)

                                                        TTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGG                                       >  SRR4302535.4647575/1‑94 (MQ=60)

                                                        TTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGG                                       <  SRR4302535.3858946/94‑1 (MQ=60)

                                                        TTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGG                                       >  SRR4302535.4158258/1‑94 (MQ=60)

                                                         TATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGT                                      <  SRR4302535.524243/94‑1 (MQ=60)

                                                           TGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTA                                    <  SRR4302535.2796431/94‑1 (MQ=60)

                                                            GCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAA                                   <  SRR4302535.3913212/94‑1 (MQ=60)

                                                                AAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAA                               <  SRR4302535.4597664/94‑1 (MQ=60)

                                                                AAGCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAA                               >  SRR4302535.643229/1‑94 (MQ=60)

                                                                 AGCCAGAAAAGCCGCTGCCAGTATGTCGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAG                              <  SRR4302535.257138/94‑1 (MQ=60)

                                                                  GCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGG                             <  SRR4302535.173887/94‑1 (MQ=60)

                                                                  GCCAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGG                             <  SRR4302535.2745733/94‑1 (MQ=60)

                                                                    CAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAA                           >  SRR4302535.823232/1‑94 (MQ=60)

                                                                    CAGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAA                           >  SRR4302535.1665760/1‑94 (MQ=60)

                                                                     AGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAA                          >  SRR4302535.2920590/1‑94 (MQ=60)

                                                                     AGAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAA                          >  SRR4302535.3637763/1‑94 (MQ=60)

                                                                      GAAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAG                         <  SRR4302535.3210705/94‑1 (MQ=60)

                                                                       AAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGA                        >  SRR4302535.438098/1‑94 (MQ=60)

                                                                       AAAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCAGAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGA                        >  SRR4302535.2530627/1‑94 (MQ=60)

                                                                        AAAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAA                       <  SRR4302535.817207/94‑1 (MQ=60)

                                                                         AAGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAG                      >  SRR4302535.1744263/1‑94 (MQ=60)

                                                                          AGCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGA                     <  SRR4302535.1425762/94‑1 (MQ=60)

                                                                           GCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGAT                    >  SRR4302535.3476318/1‑94 (MQ=60)

                                                                           GCCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGAT                    <  SRR4302535.833159/94‑1 (MQ=60)

                                                                            CCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGGGGGTTAAATAAGGAAAGAAGATG                   <  SRR4302535.818790/94‑1 (MQ=60)

                                                                            CCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATG                   <  SRR4302535.30819/94‑1 (MQ=60)

                                                                            CCGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATCCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATG                   <  SRR4302535.2528197/94‑1 (MQ=60)

                                                                             CGCTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGA                  >  SRR4302535.4158444/1‑94 (MQ=60)

                                                                               CTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATT                >  SRR4302535.2157762/1‑94 (MQ=60)

                                                                               CTGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATT                >  SRR4302535.894196/1‑94 (MQ=60)

                                                                                TGCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTG               >  SRR4302535.4538350/1‑94 (MQ=60)

                                                                                 GCCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGA              >  SRR4302535.1276252/1‑94 (MQ=60)

                                                                                  CCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAA             >  SRR4302535.936925/1‑94 (MQ=60)

                                                                                  CCAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAA             <  SRR4302535.1408498/94‑1 (MQ=60)

                                                                                   CAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAAC            <  SRR4302535.3404980/94‑1 (MQ=60)

                                                                                   CAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAAC            <  SRR4302535.3344237/94‑1 (MQ=60)

                                                                                   CAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAAC            >  SRR4302535.4144237/1‑94 (MQ=60)

                                                                                   CAGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAAC            <  SRR4302535.4407956/94‑1 (MQ=60)

                                                                                    AGTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACA           <  SRR4302535.550720/94‑1 (MQ=60)

                                                                                     GTATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAA          >  SRR4302535.864209/1‑94 (MQ=60)

                                                                                      TATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAAT         <  SRR4302535.3434643/94‑1 (MQ=60)

                                                                                       ATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATT        <  SRR4302535.3720532/94‑1 (MQ=60)

                                                                                       ATGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATT        <  SRR4302535.4623903/94‑1 (MQ=60)

                                                                                        TGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTT       >  SRR4302535.3937737/1‑94 (MQ=60)

                                                                                        TGTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTT       >  SRR4302535.2258512/1‑94 (MQ=60)

                                                                                         GTGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTT      <  SRR4302535.516233/94‑1 (MQ=60)

                                                                                          TGGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTT     <  SRR4302535.2465205/94‑1 (MQ=60)

                                                                                           GGTGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTC    >  SRR4302535.2942736/1‑94 (MQ=60)

                                                                                             TGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAG  <  SRR4302535.55335/94‑1 (MQ=60)

GTCAATGGTGTGATGGTCTATGAAGACCGTCAGTTTAACTTCGATTGCGATTCCATTTATGCACAAGCCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATGACAGATGCCCTCTTCCCGCAGGGAAGAGGGTTAAATAAGGAAAGAAGATGATTGAACAATTTTTCAG  >  NC_000913/3018394‑3018580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`