Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,676	+C	coding (535/570 nt)	ygfQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,675	1	.	C	100.0%	399.1 / NA	125	L178F (TTG→TTC)	ygfQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (63/62); total (63/62)

Only 100 of 135 total aligned reads displayed.

CTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGC  >  NC_000913/3025584‑3025761

 tCACGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑cc                                                                                      >  1:3684256/1‑92 (MQ=255)

   aCGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑ccag                                                                                    >  2:2578418/1‑90 (MQ=255)

   aCGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑ccag                                                                                    >  2:1080948/1‑90 (MQ=255)

    cGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑ccagt                                                                                   >  2:3587103/1‑89 (MQ=255)

      gTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTgg                                                                                  >  2:578761/1‑93 (MQ=255)

      gTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTgg                                                                                  >  2:2748396/1‑93 (MQ=255)

           ggtatcggcatcGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTc                                                                             <  1:1605924/93‑1 (MQ=255)

             tatcggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCgg                                                                           <  2:1023354/93‑1 (MQ=255)

              atcggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGt                                                                          <  1:1919412/93‑1 (MQ=255)

                cggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGc                                                                        <  2:4309932/93‑1 (MQ=255)

                cggcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGc                                                                        <  2:179024/93‑1 (MQ=255)

                  gcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGt                                                                      >  2:419094/3‑93 (MQ=255)

                  gcatcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGt                                                                      >  2:2940070/3‑93 (MQ=255)

                   catcggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGtt                                                                     <  1:1684906/92‑1 (MQ=255)

                      cggGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAc                                                                  <  2:3161451/93‑1 (MQ=255)

                       gggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcc                                                                 >  1:1867855/1‑93 (MQ=255)

                       gggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcc                                                                 <  1:2092396/93‑1 (MQ=255)

                       gggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcc                                                                 <  2:4258336/93‑1 (MQ=255)

                       gggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcc                                                                 <  2:3356438/93‑1 (MQ=255)

                       cggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcc                                                                 <  2:3102381/92‑1 (MQ=255)

                        ggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcct                                                                >  1:3971137/1‑93 (MQ=255)

                        ggCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcct                                                                <  2:4320731/93‑1 (MQ=255)

                         gCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctc                                                               <  1:495278/93‑1 (MQ=255)

                         gCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctc                                                               >  2:4139946/1‑93 (MQ=255)

                         gCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctc                                                               <  2:1931929/93‑1 (MQ=255)

                          cTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcc                                                              >  2:2733489/1‑93 (MQ=255)

                          cTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcc                                                              >  1:4173239/1‑93 (MQ=255)

                           tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcct                                                             <  2:2782978/93‑1 (MQ=255)

                           tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcct                                                             >  1:1784169/1‑93 (MQ=255)

                           tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcct                                                             <  2:1134736/93‑1 (MQ=255)

                           tGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTAcctcct                                                             >  1:4278890/1‑93 (MQ=255)

                            gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                            <  1:2434538/93‑1 (MQ=255)

                            gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                            >  1:4309319/1‑93 (MQ=255)

                            gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                            >  2:4654472/1‑93 (MQ=255)

                            gTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGGATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCtt                                                            >  2:3006204/1‑93 (MQ=255)

                             tttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTc                                                           >  2:637212/1‑93 (MQ=255)

                              ttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTcc                                                          >  1:443495/1‑93 (MQ=255)

                              ttCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTcc                                                          <  2:1515654/93‑1 (MQ=255)

                                ctgctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCg                                                        >  2:3766811/1‑93 (MQ=255)

                                 tgctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCgg                                                       >  1:3211076/1‑93 (MQ=255)

                                  gctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGt                                                      >  2:151021/1‑93 (MQ=255)

                                  gctgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGt                                                      <  1:3817565/93‑1 (MQ=255)

                                    tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                    <  2:2408107/93‑1 (MQ=255)

                                    tgctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtga                                                    >  1:2037788/1‑93 (MQ=255)

                                      ctgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGCTTACCTCCTTCCCGGtgatg                                                  >  2:4065270/1‑93 (MQ=255)

                                       tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                 >  2:3719081/1‑93 (MQ=255)

                                       tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                 >  2:2570135/1‑93 (MQ=255)

                                       tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                 <  2:1143614/93‑1 (MQ=255)

                                       tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                 <  2:3376932/93‑1 (MQ=255)

                                       tgATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatga                                                 >  1:4577545/1‑93 (MQ=255)

                                        gATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATGGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGtgatgat                                                >  1:2511695/1‑93 (MQ=255)

                                           tgctgcTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCGCGGTGATGATGAg                                             <  1:14319/93‑1 (MQ=255)

                                            gctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGc                                            >  2:3519621/1‑93 (MQ=255)

                                            gctgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGc                                            <  2:7558/93‑1 (MQ=255)

                                              tgctAGCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCtt                                          <  2:4161890/93‑1 (MQ=255)

                                              tgctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCtt                                          <  1:2233476/93‑1 (MQ=255)

                                                ctAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGc                                        >  2:4472980/1‑93 (MQ=255)

                                                 tAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCt                                       >  1:3584348/1‑93 (MQ=255)

                                                   aCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTgg                                     >  1:3363431/1‑93 (MQ=255)

                                                   aCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTgg                                     <  2:2119324/93‑1 (MQ=255)

                                                   aCGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTgg                                     >  1:791085/1‑93 (MQ=255)

                                                      gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                  <  1:3373105/93‑1 (MQ=255)

                                                      gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                  <  1:2790063/93‑1 (MQ=255)

                                                      gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                  >  2:2665112/1‑93 (MQ=255)

                                                      gtgtGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGc                                  <  1:2446579/93‑1 (MQ=255)

                                                       tgtgGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCt                                 <  1:3771859/93‑1 (MQ=255)

                                                           ggTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGCGATGATGAGCTTGCTGGGGCTGGCg                             >  1:977642/1‑93 (MQ=255)

                                                            gTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                            >  1:3326086/1‑93 (MQ=255)

                                                            gTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCgg                            >  2:3175052/1‑93 (MQ=255)

                                                             tATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGt                           >  1:647523/1‑93 (MQ=255)

                                                               tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCa                         >  1:859414/1‑93 (MQ=255)

                                                               tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCa                         >  2:4089480/1‑93 (MQ=255)

                                                               tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCa                         <  2:4471393/93‑1 (MQ=255)

                                                               tGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGAGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCa                         >  2:334597/1‑93 (MQ=255)

                                                                 gTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATc                       >  1:192682/1‑93 (MQ=255)

                                                                  ttATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCt                      >  1:3682956/1‑93 (MQ=255)

                                                                  ttATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCt                      <  1:1666268/93‑1 (MQ=255)

                                                                   tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                     >  2:1346110/1‑93 (MQ=255)

                                                                   tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                     <  2:2340897/93‑1 (MQ=255)

                                                                   tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCtt                     >  1:4652774/1‑93 (MQ=255)

                                                                   tATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGAGGCTGGCGGCCATCtt                     >  1:1420894/1‑93 (MQ=255)

                                                                     tCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCg                   <  1:1890006/93‑1 (MQ=255)

                                                                      cAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCgg                  >  2:3679142/1‑93 (MQ=255)

                                                                       aaaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGc                 <  1:312672/93‑1 (MQ=255)

                                                                       aaaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGc                 >  2:2120643/1‑93 (MQ=255)

                                                                        aaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGcc                >  2:3151712/1‑93 (MQ=255)

                                                                        aaaaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGcc                <  2:1985062/93‑1 (MQ=255)

                                                                          aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg              <  1:2988492/93‑1 (MQ=255)

                                                                          aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg              <  2:1097312/93‑1 (MQ=255)

                                                                          aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTg              >  2:1389298/1‑93 (MQ=255)

                                                                          aaCCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGGTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTGATCTTCGGGCTg              >  2:7267/1‑93 (MQ=255)

                                                                            cccGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGga            >  1:1630877/1‑93 (MQ=255)

                                                                            cccGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGga            >  1:4122014/1‑93 (MQ=255)

                                                                             ccGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGgag           >  2:630944/1‑93 (MQ=255)

                                                                              cGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGgaga          <  1:3962141/93‑1 (MQ=255)

                                                                                 ttGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgg       >  1:1115795/1‑92 (MQ=255)

                                                                                  tGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtg      <  1:843093/93‑1 (MQ=255)

                                                                                   gAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAgtgt     <  2:2398036/93‑1 (MQ=255)

                                                                                     aGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcg   <  1:3070380/93‑1 (MQ=255)

                                                                                      ggCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTcgc  <  2:409166/93‑1 (MQ=255)

CTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGC  >  NC_000913/3025584‑3025761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGG  >  NC_000913/3025584‑3025769

CTCACGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CC                                                                                              >  SRR4302535.3709748/1‑94 (MQ=60)

 TCACGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑Cca                                                                                             >  SRR4302535.828623/1‑92 (MQ=60)

   ACTGTACAGGTATCGGCATCGGGCTGTTGCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGT                                                                                            <  SRR4302535.2018781/94‑1 (MQ=60)

    CGGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGGGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTG                                                                                           >  SRR4302535.980776/1‑94 (MQ=60)

     GGTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGG                                                                                          <  SRR4302535.4542659/94‑1 (MQ=60)

      GTACAGGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTACCAAAAACCCGATTGAAGGCTTGCCAGTGGC                                                                                         <  SRR4302535.1986649/94‑1 (MQ=60)

           GGTATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCG                                                                                    <  SRR4302535.1617512/94‑1 (MQ=60)

              ATCGGCATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTG                                                                                 <  SRR4302535.1933247/94‑1 (MQ=60)

                   CATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTT                                                                            <  SRR4302535.1697106/94‑1 (MQ=60)

                   CATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTT                                                                            <  SRR4302535.3990889/94‑1 (MQ=60)

                    ATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTA                                                                           <  SRR4302535.233733/94‑1 (MQ=60)

                    ATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTA                                                                           >  SRR4302535.1884306/1‑94 (MQ=60)

                      CGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACC                                                                         >  SRR4302535.1881318/1‑94 (MQ=60)

                       GGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCT                                                                        >  SRR4302535.3998261/1‑94 (MQ=60)

                       GGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCT                                                                        <  SRR4302535.2107472/94‑1 (MQ=60)

                         GCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCC                                                                      >  SRR4302535.4201803/1‑94 (MQ=60)

                         GCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCC                                                                      <  SRR4302535.498754/94‑1 (MQ=60)

                          CTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCT                                                                     >  SRR4302535.4308171/1‑94 (MQ=60)

                          CTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCT                                                                     >  SRR4302535.1797081/1‑94 (MQ=60)

                           TGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTT                                                                    >  SRR4302535.4338800/1‑94 (MQ=60)

                            GTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTC                                                                   <  SRR4302535.2452076/94‑1 (MQ=60)

                             TTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCC                                                                  >  SRR4302535.446599/1‑94 (MQ=60)

                             TTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCC                                                                  >  SRR4302535.4513346/1‑94 (MQ=60)

                                CTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGG                                                               >  SRR4302535.3233316/1‑94 (MQ=60)

                                  GCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTCCCTCCTTCCCGGTG                                                             <  SRR4302535.161549/94‑1 (MQ=60)

                                  GCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTG                                                             <  SRR4302535.3843911/94‑1 (MQ=60)

                                  GCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTG                                                             <  SRR4302535.3080516/94‑1 (MQ=60)

                                   CTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGA                                                            <  SRR4302535.3709852/94‑1 (MQ=60)

                                   CTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGA                                                            >  SRR4302535.2052464/1‑94 (MQ=60)

                                    TGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGAT                                                           <  SRR4302535.4308040/94‑1 (MQ=60)

                                      CTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGA                                                         >  SRR4302535.4608730/1‑94 (MQ=60)

                                       TGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATGGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGAT                                                        >  SRR4302535.2529799/1‑94 (MQ=60)

                                           TGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCGCGGTGATGATGAGC                                                    <  SRR4302535.14422/94‑1 (MQ=60)

                                             CTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTT                                                  <  SRR4302535.1688600/94‑1 (MQ=60)

                                              TGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTG                                                 <  SRR4302535.2249360/94‑1 (MQ=60)

                                                CTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCT                                               >  SRR4302535.3609207/1‑94 (MQ=60)

                                                  AACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGG                                             >  SRR4302535.796638/1‑94 (MQ=60)

                                                  AACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGG                                             >  SRR4302535.3386834/1‑94 (MQ=60)

                                                      GTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCT                                         <  SRR4302535.2464201/94‑1 (MQ=60)

                                                      GTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCT                                         <  SRR4302535.3396568/94‑1 (MQ=60)

                                                      GTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCT                                         <  SRR4302535.2809920/94‑1 (MQ=60)

                                                       TGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTG                                        <  SRR4302535.3797879/94‑1 (MQ=60)

                                                          GGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCG                                     <  SRR4302535.4718033/94‑1 (MQ=60)

                                                          GGGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGCGATGATGAGCTTGCTGGGGCTGGCG                                     >  SRR4302535.984588/1‑94 (MQ=60)

                                                           GGTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGG                                    >  SRR4302535.3349231/1‑94 (MQ=60)

                                                            GTATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGT                                   >  SRR4302535.652037/1‑94 (MQ=60)

                                                              ATGGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCA                                 >  SRR4302535.865480/1‑94 (MQ=60)

                                                                GGTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATC                               >  SRR4302535.194009/1‑94 (MQ=60)

                                                                 GTTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCT                              >  SRR4302535.3708442/1‑94 (MQ=60)

                                                                  TTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTT                             <  SRR4302535.1678323/94‑1 (MQ=60)

                                                                  TTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTT                             >  SRR4302535.4684277/1‑94 (MQ=60)

                                                                  TTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTT                             >  SRR4302535.2767754/1‑94 (MQ=60)

                                                                  TTATCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGAGGCTGGCGGCCATCTT                             >  SRR4302535.1431038/1‑94 (MQ=60)

                                                                     TCAAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGG                          <  SRR4302535.1903619/94‑1 (MQ=60)

                                                                       AAAAACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCC                        <  SRR4302535.314838/94‑1 (MQ=60)

                                                                          AACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGG                     <  SRR4302535.3009446/94‑1 (MQ=60)

                                                                           ACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGA                    >  SRR4302535.4150221/1‑94 (MQ=60)

                                                                           ACCCGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGA                    >  SRR4302535.1642653/1‑94 (MQ=60)

                                                                              CGATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAA                 <  SRR4302535.3989203/94‑1 (MQ=60)

                                                                                ATTGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGG               >  SRR4302535.1123750/1‑94 (MQ=60)

                                                                                 TTGAAGGCTTGCCGGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTG              <  SRR4302535.327153/94‑1 (MQ=60)

                                                                                  TGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGGAGTGT             >  SRR4302535.3484951/1‑94 (MQ=60)

                                                                                  TGAAGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGT             <  SRR4302535.849016/94‑1 (MQ=60)

                                                                                     AGGCTTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGC          <  SRR4302535.3091822/94‑1 (MQ=60)

                                                                                         TTGCCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTAC      >  SRR4302535.886896/1‑94 (MQ=60)

                                                                                          tgcCAGTGTCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACC     <  SRR4302535.1413280/91‑1 (MQ=60)

                                                                                           gcCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCC    >  SRR4302535.2603761/3‑94 (MQ=60)

                                                                                           gcCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCC    >  SRR4302535.2855730/3‑94 (MQ=60)

                                                                                           gcCAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCC    >  SRR4302535.3162905/3‑94 (MQ=60)

                                                                                             CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGG  >  SRR4302535.2324106/1‑94 (MQ=60)

CTCACGGTACAGGTATCGGTATCGGGCTGTTTCTGCTGCTGATTGCTGCTAACGGTGTGGGTATGGTTATCAAAAACCCGATTGAAGGCTTG‑CAGTGGCGCTCGGTGCGTTTACCTCCTTCCCGGTGATGATGAGCTTGCTGGGGCTGGCGGTCATCTTCGGCCTGGAGAAGTGTCGCGTACCCGG  >  NC_000913/3025584‑3025769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`