Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,064,509 T→C M229V (ATG→GTG)  b2921 ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,064,5090TC99.6% 775.0 / ‑5.2 244M229V (ATG→GTG) b2921putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (99/144);  total (99/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

Only 100 of 245 total aligned reads displayed.

ATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCT  >  NC_000913/3064419‑3064598
                                                                                          |                                                                                         
atTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACaa                                                                                         <  2:2228522/93‑1 (MQ=255)
 tttCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACaaa                                                                                        >  2:122096/1‑93 (MQ=255)
  ttCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACaaaa                                                                                       >  1:2117133/1‑93 (MQ=255)
    cAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACt                                                                                     <  2:157036/93‑1 (MQ=255)
    cAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACt                                                                                     >  1:1024950/1‑93 (MQ=255)
    cAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACt                                                                                     >  1:3130012/1‑93 (MQ=255)
    cAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACt                                                                                     <  2:3847128/93‑1 (MQ=255)
     aGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTg                                                                                    <  2:2079762/93‑1 (MQ=255)
     aGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTg                                                                                    <  2:4246208/93‑1 (MQ=255)
     aGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTC‑‑AAAATGTGCGCGCTCCCCGGGGCTGATTTTGGCCACAAAAcacta                                                                                  >  1:2634397/1‑89 (MQ=255)
      gCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGt                                                                                   <  1:505512/93‑1 (MQ=255)
       cGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTa                                                                                  <  1:2894403/93‑1 (MQ=255)
        gTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAg                                                                                 <  1:4228887/93‑1 (MQ=255)
        gTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAg                                                                                 >  2:4479946/1‑93 (MQ=255)
         ttGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAgg                                                                                <  2:3681937/93‑1 (MQ=255)
            ccGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGAc                                                                             <  2:1961274/93‑1 (MQ=255)
             cGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACg                                                                            >  1:503201/1‑93 (MQ=255)
             cGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACg                                                                            >  1:1190143/1‑93 (MQ=255)
              ggATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGa                                                                           <  1:3643071/93‑1 (MQ=255)
              ggATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGa                                                                           <  2:2200268/93‑1 (MQ=255)
               gATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGat                                                                          <  1:4472598/93‑1 (MQ=255)
               gATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGat                                                                          <  1:1992005/93‑1 (MQ=255)
               gATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGat                                                                          >  1:408686/1‑93 (MQ=255)
               gATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGat                                                                          <  1:1936228/93‑1 (MQ=255)
               gATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGat                                                                          >  2:4622924/1‑93 (MQ=255)
               gATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGat                                                                          >  2:1775542/1‑93 (MQ=255)
               gATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGat                                                                          <  1:879798/93‑1 (MQ=255)
                 ttCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGataa                                                                        >  1:2486073/1‑93 (MQ=255)
                    aCCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGataaata                                                                     <  2:228574/93‑1 (MQ=255)
                     ccAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGataaataa                                                                    >  2:597322/1‑93 (MQ=255)
                     ccAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGataaataa                                                                    <  1:918457/93‑1 (MQ=255)
                     ccAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGataaataa                                                                    <  2:4194335/93‑1 (MQ=255)
                     ccAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGataaataa                                                                    <  1:3923131/93‑1 (MQ=255)
                        gTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATgg                                                                 >  1:3191742/1‑93 (MQ=255)
                         tCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGGGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGt                                                                <  2:1121750/93‑1 (MQ=255)
                         tCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGt                                                                <  1:3485454/93‑1 (MQ=255)
                         tCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGt                                                                <  1:4036016/93‑1 (MQ=255)
                         tCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGt                                                                >  2:3736444/1‑93 (MQ=255)
                          cGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGtt                                                               <  2:138623/93‑1 (MQ=255)
                           gCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTg                                                              >  1:4544650/1‑93 (MQ=255)
                             cAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGa                                                            >  2:1575989/1‑93 (MQ=255)
                             cAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGa                                                            >  2:345291/1‑93 (MQ=255)
                             cAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGa                                                            <  2:3135564/93‑1 (MQ=255)
                              aGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGAt                                                           >  1:3644622/1‑93 (MQ=255)
                              aGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGAt                                                           <  2:2646139/93‑1 (MQ=255)
                              aGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGAt                                                           <  1:332253/93‑1 (MQ=255)
                              aGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGAt                                                           <  1:3948005/93‑1 (MQ=255)
                               agCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGAtt                                                          <  2:1192568/92‑1 (MQ=255)
                                 gaaaaTGCGGAAGCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGa                                                        <  2:2521327/92‑1 (MQ=255)
                                 cAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGa                                                        <  1:4276028/93‑1 (MQ=255)
                                  aaaaTGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGAt                                                       >  1:1215884/1‑93 (MQ=255)
                                    aaTGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATcc                                                     <  1:3712104/93‑1 (MQ=255)
                                     aTGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATcca                                                    >  2:3951079/1‑92 (MQ=255)
                                       gCGGGATCACCGTCAGAAAATGTCCGCGCTCAACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCcac                                                  <  2:2369453/93‑1 (MQ=255)
                                          ggATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAg                                               <  2:4387839/93‑1 (MQ=255)
                                           gATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAgg                                              >  1:1327331/1‑93 (MQ=255)
                                            aTCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGt                                             <  1:3816813/93‑1 (MQ=255)
                                            aTCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGt                                             >  1:3811007/1‑93 (MQ=255)
                                            aTCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACAATAAATAATGGTTGGATTGATCCCACAAGGt                                             <  2:871635/93‑1 (MQ=255)
                                             tCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTc                                            >  2:3490855/1‑93 (MQ=255)
                                              cACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCg                                           >  1:3401644/1‑93 (MQ=255)
                                              cACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCg                                           >  2:737017/1‑93 (MQ=255)
                                               aCCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGc                                          <  1:1735406/93‑1 (MQ=255)
                                               aCCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGc                                          <  1:4571371/93‑1 (MQ=255)
                                                ccGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCa                                         <  1:3114572/93‑1 (MQ=255)
                                                ccGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCa                                         <  2:3770376/93‑1 (MQ=255)
                                                ccGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCa                                         <  1:4458939/93‑1 (MQ=255)
                                                ccGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCa                                         >  1:793778/1‑93 (MQ=255)
                                                ccGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCa                                         <  1:903130/93‑1 (MQ=255)
                                                  gTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCata                                       <  1:584797/93‑1 (MQ=255)
                                                   tCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCatat                                      >  1:1525256/1‑93 (MQ=255)
                                                    cAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATAtt                                     <  1:4345110/93‑1 (MQ=255)
                                                    cAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATAtt                                     <  1:2225017/93‑1 (MQ=255)
                                                     aGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTc                                    <  2:1488748/93‑1 (MQ=255)
                                                      gAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCg                                   >  1:3010119/1‑93 (MQ=255)
                                                       aaaaTGTCCGCTCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGc                                  <  1:184018/93‑1 (MQ=255)
                                                        aaaTGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCa                                 >  2:2675593/1‑93 (MQ=255)
                                                         agtGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCat                                <  1:1614001/91‑1 (MQ=255)
                                                         aaTGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCat                                <  1:1784837/93‑1 (MQ=255)
                                                         aaTGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCat                                <  1:1691848/93‑1 (MQ=255)
                                                         aaTGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCat                                <  2:2835077/93‑1 (MQ=255)
                                                          aTGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCatc                               <  1:999774/93‑1 (MQ=255)
                                                               cgcgCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCatcaatca                          <  2:1315039/93‑1 (MQ=255)
                                                               cgcgCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCatcaatca                          <  2:2906389/93‑1 (MQ=255)
                                                               cgcgCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCatcaatca                          >  1:684979/1‑93 (MQ=255)
                                                                gcgcTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCAt                         <  1:2564661/93‑1 (MQ=255)
                                                                 cgcTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCAtt                        <  2:3169036/93‑1 (MQ=255)
                                                                   cTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGc                      <  1:752935/93‑1 (MQ=255)
                                                                    tCCACGGCGCTGATTTTGGCCACAAAACTGTCGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCt                     >  2:3754957/1‑93 (MQ=255)
                                                                     ccgcGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCtt                    <  1:1914897/90‑1 (MQ=255)
                                                                         ggCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGaa                <  1:2935860/93‑1 (MQ=255)
                                                                          gcgcTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAg               <  2:3304862/93‑1 (MQ=255)
                                                                           cgcTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGt              >  2:3083216/1‑93 (MQ=255)
                                                                            gcTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGtt             >  2:2400354/1‑93 (MQ=255)
                                                                              tGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTgg           >  1:139699/1‑93 (MQ=255)
                                                                               gATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGc          >  2:3831488/1‑93 (MQ=255)
                                                                                   ttGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTGGAAGTTGGCCCgg      <  2:1856122/93‑1 (MQ=255)
                                                                                      gCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTc   <  2:4163727/93‑1 (MQ=255)
                                                                                      gCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTc   <  2:56157/93‑1 (MQ=255)
                                                                                       ccACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCt  <  1:2372914/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCT  >  NC_000913/3064419‑3064598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTGAA  >  NC_000913/3064417‑3064601
                                                                                            |                                                                                            
ATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACA                                                                                             <  SRR4302535.3824592/94‑1 (MQ=60)
 TATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAA                                                                                            <  SRR4302535.242911/94‑1 (MQ=60)
  ATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAA                                                                                           >  SRR4302535.1167892/1‑94 (MQ=60)
  ATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAA                                                                                           >  SRR4302535.4083764/1‑94 (MQ=60)
   TTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAA                                                                                          >  SRR4302535.2132405/1‑94 (MQ=60)
   TTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAA                                                                                          <  SRR4302535.2633008/94‑1 (MQ=60)
    TTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAAC                                                                                         <  SRR4302535.1321042/94‑1 (MQ=60)
    TTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAAC                                                                                         >  SRR4302535.4228906/1‑94 (MQ=60)
     TCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACT                                                                                        >  SRR4302535.1032232/1‑94 (MQ=60)
     TCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACT                                                                                        >  SRR4302535.3151811/1‑94 (MQ=60)
      CAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTG                                                                                       <  SRR4302535.2454457/94‑1 (MQ=60)
      CAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTC‑‑AAAATGTGCGCGCTCCCCGGGGCTGATTTTGGCCACAAAACacta                                                                                     >  SRR4302535.2653277/1‑90 (MQ=60)
       AGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGT                                                                                      >  SRR4302535.1448425/1‑94 (MQ=60)
       AGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGT                                                                                      <  SRR4302535.2513430/94‑1 (MQ=60)
        GCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTA                                                                                     <  SRR4302535.509045/94‑1 (MQ=60)
         CGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAG                                                                                    <  SRR4302535.894074/94‑1 (MQ=60)
         CGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAG                                                                                    <  SRR4302535.2914876/94‑1 (MQ=60)
          GTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGG                                                                                   <  SRR4302535.4257827/94‑1 (MQ=60)
           TTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGG                                                                                  <  SRR4302535.2173167/94‑1 (MQ=60)
           GTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGG                                                                                  <  SRR4302535.2576986/94‑1 (MQ=60)
              CCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACG                                                                               >  SRR4302535.1198664/1‑94 (MQ=60)
              CCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACG                                                                               >  SRR4302535.506720/1‑94 (MQ=60)
              CCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACG                                                                               <  SRR4302535.2131523/94‑1 (MQ=60)
               CGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGA                                                                              <  SRR4302535.2670595/94‑1 (MQ=60)
                GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGAT                                                                             >  SRR4302535.778297/1‑94 (MQ=60)
                GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGAT                                                                             >  SRR4302535.3051226/1‑94 (MQ=60)
                GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGAT                                                                             <  SRR4302535.3668300/94‑1 (MQ=60)
                GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGAT                                                                             >  SRR4302535.699721/1‑94 (MQ=60)
                GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGAT                                                                             >  SRR4302535.533199/1‑94 (MQ=60)
                GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGAT                                                                             >  SRR4302535.411552/1‑94 (MQ=60)
                GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGAT                                                                             >  SRR4302535.98673/1‑94 (MQ=60)
                GGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGAT                                                                             >  SRR4302535.1560921/1‑94 (MQ=60)
                 GATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATA                                                                            <  SRR4302535.1950173/94‑1 (MQ=60)
                 GATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATA                                                                            <  SRR4302535.2006346/94‑1 (MQ=60)
                 GATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATA                                                                            <  SRR4302535.4503293/94‑1 (MQ=60)
                 GATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATA                                                                            <  SRR4302535.886027/94‑1 (MQ=60)
                  ATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAA                                                                           >  SRR4302535.4644560/1‑94 (MQ=60)
                  ATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAA                                                                           >  SRR4302535.2503998/1‑94 (MQ=60)
                    TCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAAT                                                                         <  SRR4302535.4497886/94‑1 (MQ=60)
                    TCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAAT                                                                         <  SRR4302535.3651724/94‑1 (MQ=60)
                     CACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATA                                                                        >  SRR4302535.799914/1‑94 (MQ=60)
                       CCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAATCTGTAGGGACGATAAATAAT                                                                      >  SRR4302535.3147326/1‑94 (MQ=60)
                       CCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAAT                                                                      <  SRR4302535.924970/94‑1 (MQ=60)
                       CCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAAT                                                                      <  SRR4302535.3949943/94‑1 (MQ=60)
                       CCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAAT                                                                      >  SRR4302535.3788749/1‑94 (MQ=60)
                       CCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAAT                                                                      >  SRR4302535.3370480/1‑94 (MQ=60)
                       CCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAAT                                                                      <  SRR4302535.2926909/94‑1 (MQ=60)
                        CAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATG                                                                     >  SRR4302535.4286271/1‑94 (MQ=60)
                        CAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATG                                                                     <  SRR4302535.1302168/94‑1 (MQ=60)
                         AGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGG                                                                    >  SRR4302535.2361092/1‑94 (MQ=60)
                         AGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGG                                                                    <  SRR4302535.1945713/94‑1 (MQ=60)
                         AGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGG                                                                    >  SRR4302535.3213819/1‑94 (MQ=60)
                         AGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGG                                                                    <  SRR4302535.3005345/94‑1 (MQ=60)
                          GTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGT                                                                   >  SRR4302535.3396412/1‑94 (MQ=60)
                           TCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTT                                                                  <  SRR4302535.4063644/94‑1 (MQ=60)
                           TCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTT                                                                  <  SRR4302535.3509652/94‑1 (MQ=60)
                            CGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTG                                                                 >  SRR4302535.4575731/1‑94 (MQ=60)
                            CGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTG                                                                 >  SRR4302535.1652886/1‑94 (MQ=60)
                             GCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGG                                                                >  SRR4302535.1360678/1‑94 (MQ=60)
                             GCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGG                                                                <  SRR4302535.1773526/94‑1 (MQ=60)
                              CCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGA                                                               <  SRR4302535.4446784/94‑1 (MQ=60)
                               CAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGAT                                                              >  SRR4302535.3669863/1‑94 (MQ=60)
                                AGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATT                                                             <  SRR4302535.3974958/94‑1 (MQ=60)
                                AGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATT                                                             <  SRR4302535.334552/94‑1 (MQ=60)
                                AGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATT                                                             <  SRR4302535.681009/94‑1 (MQ=60)
                                 GGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTG                                                            <  SRR4302535.3450105/94‑1 (MQ=60)
                                 GGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTG                                                            <  SRR4302535.3275583/94‑1 (MQ=60)
                                 GGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTG                                                            <  SRR4302535.4191016/94‑1 (MQ=60)
                                  GCAAAATGCGGGATCGCCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGA                                                           <  SRR4302535.1525324/94‑1 (MQ=60)
                                   CAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGAT                                                          <  SRR4302535.4305297/94‑1 (MQ=60)
                                   CAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGAT                                                          >  SRR4302535.1224606/1‑94 (MQ=60)
                                   CAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGAT                                                          <  SRR4302535.905028/94‑1 (MQ=60)
                                     AAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCC                                                        <  SRR4302535.1829313/94‑1 (MQ=60)
                                      AATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCC                                                       <  SRR4302535.3737821/94‑1 (MQ=60)
                                       ATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCA                                                      >  SRR4302535.3866952/1‑94 (MQ=60)
                                        TGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCAC                                                     <  SRR4302535.4588068/94‑1 (MQ=60)
                                         GCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACA                                                    >  SRR4302535.3727378/1‑94 (MQ=60)
                                            GGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGG                                                 <  SRR4302535.3793375/94‑1 (MQ=60)
                                            GGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGG                                                 <  SRR4302535.918506/94‑1 (MQ=60)
                                            GGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGG                                                 >  SRR4302535.1336801/1‑94 (MQ=60)
                                             GATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGT                                                >  SRR4302535.3837303/1‑94 (MQ=60)
                                             GATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGT                                                >  SRR4302535.1742217/1‑94 (MQ=60)
                                              ATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTC                                               <  SRR4302535.4327431/94‑1 (MQ=60)
                                              ATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTC                                               <  SRR4302535.3843149/94‑1 (MQ=60)
                                               TCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCG                                              >  SRR4302535.3425317/1‑94 (MQ=60)
                                                 ACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCA                                            >  SRR4302535.799355/1‑94 (MQ=60)
                                                 ACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCA                                            <  SRR4302535.4602538/94‑1 (MQ=60)
                                                 ACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCA                                            <  SRR4302535.1747952/94‑1 (MQ=60)
                                                  CCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCAT                                           <  SRR4302535.909526/94‑1 (MQ=60)
                                                  CCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCAT                                           <  SRR4302535.4489520/94‑1 (MQ=60)
                                                  CCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCAT                                           <  SRR4302535.3136256/94‑1 (MQ=60)
                                                    GTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATAT                                         >  SRR4302535.1536246/1‑94 (MQ=60)
                                                    GTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATAT                                         <  SRR4302535.588897/94‑1 (MQ=60)
                                                    GTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATAT                                         <  SRR4302535.4052476/94‑1 (MQ=60)
                                                    GTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATAT                                         >  SRR4302535.4534891/1‑94 (MQ=60)
                                                      CAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTC                                       <  SRR4302535.2240849/94‑1 (MQ=60)
                                                      CAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTC                                       <  SRR4302535.4374838/94‑1 (MQ=60)
                                                       AGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCG                                      >  SRR4302535.3031203/1‑94 (MQ=60)
                                                       AGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCG                                      >  SRR4302535.3886126/1‑94 (MQ=60)
                                                        GAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGC                                     <  SRR4302535.3840509/94‑1 (MQ=60)
                                                         AAAATGTCCGCTCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCA                                    <  SRR4302535.185284/94‑1 (MQ=60)
                                                          AAATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCAT                                   <  SRR4302535.4016229/94‑1 (MQ=60)
                                                           AGTGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATC                                  <  SRR4302535.1625633/94‑1 (MQ=60)
                                                           AATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATC                                  <  SRR4302535.1797756/94‑1 (MQ=60)
                                                           AATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATC                                  <  SRR4302535.1704087/94‑1 (MQ=60)
                                                            ATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCA                                 <  SRR4302535.1006882/94‑1 (MQ=60)
                                                            ATGTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCA                                 >  SRR4302535.1903425/1‑94 (MQ=60)
                                                              GTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAAT                               >  SRR4302535.963591/1‑94 (MQ=60)
                                                              GTCCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAAT                               >  SRR4302535.728841/1‑94 (MQ=60)
                                                                CCGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCA                             >  SRR4302535.689778/1‑94 (MQ=60)
                                                                 CGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCAT                            <  SRR4302535.1665807/94‑1 (MQ=60)
                                                                 CGCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCAT                            <  SRR4302535.4545788/94‑1 (MQ=60)
                                                                  GCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATT                           >  SRR4302535.2275638/1‑94 (MQ=60)
                                                                  GCGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATT                           <  SRR4302535.2583120/94‑1 (MQ=60)
                                                                   CGCTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTG                          >  SRR4302535.50718/1‑94 (MQ=60)
                                                                     CTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCT                        >  SRR4302535.1205623/1‑94 (MQ=60)
                                                                     CTCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCT                        <  SRR4302535.758234/94‑1 (MQ=60)
                                                                      TCCACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTT                       <  SRR4302535.1945553/94‑1 (MQ=60)
                                                                       CCGCGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTC                      <  SRR4302535.1928695/94‑1 (MQ=60)
                                                                        CACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCG                     >  SRR4302535.4317913/1‑94 (MQ=60)
                                                                        CACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCG                     <  SRR4302535.51266/94‑1 (MQ=60)
                                                                         ACGGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGA                    <  SRR4302535.3184870/94‑1 (MQ=60)
                                                                           GGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAG                  <  SRR4302535.3958291/94‑1 (MQ=60)
                                                                           GGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAG                  <  SRR4302535.1774555/94‑1 (MQ=60)
                                                                           GGCGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAG                  <  SRR4302535.2956520/94‑1 (MQ=60)
                                                                             CGCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTT                >  SRR4302535.2081992/1‑94 (MQ=60)
                                                                              GCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTG               >  SRR4302535.3195994/1‑94 (MQ=60)
                                                                              GCTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTG               <  SRR4302535.4698671/94‑1 (MQ=60)
                                                                               CTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGG              <  SRR4302535.2681056/94‑1 (MQ=60)
                                                                               CTGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGG              >  SRR4302535.140679/1‑94 (MQ=60)
                                                                                TGATTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGC             >  SRR4302535.1972842/1‑94 (MQ=60)
                                                                                   TTTTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCG          <  SRR4302535.3088435/94‑1 (MQ=60)
                                                                                     TTGGCCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGT        >  SRR4302535.3669589/1‑94 (MQ=60)
                                                                                        GCCACAAAACTGTAGGGACGATAAATAATGGTCGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCT     <  SRR4302535.365035/94‑1 (MQ=60)
                                                                                         CCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTG    >  SRR4302535.3920264/1‑94 (MQ=60)
                                                                                         CCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTG    >  SRR4302535.1169445/1‑94 (MQ=60)
                                                                                         CCACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTG    <  SRR4302535.2389957/94‑1 (MQ=60)
                                                                                          CACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTGA   >  SRR4302535.3955936/1‑94 (MQ=60)
                                                                                          CACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTGA   >  SRR4302535.1837853/1‑94 (MQ=60)
                                                                                          CACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTGA   >  SRR4302535.3642410/1‑94 (MQ=60)
                                                                                          CACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTGA   >  SRR4302535.4604845/1‑94 (MQ=60)
                                                                                           ACAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACACGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCTCGGTTCTGAA  >  SRR4302535.3009136/1‑94 (MQ=60)
                                                                                            |                                                                                            
ATATTTCAGCGTTGCCGGATTCACCAGTCGCCAGGCAAAATGCGGGATCACCGTCAGAAAATGTCCGCGCTCCACGGCGCTGATTTTGGCCATAAAACTGTAGGGACGATAAATAATGGTTGGATTGATCCCACAAGGTCGCATATTCGCATCAATCATTGCTTCGAAGTTGGCCCGGTTCTGAA  >  NC_000913/3064417‑3064601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑