Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,112,040 T→C A110A (GCA→GCG)  b2972 ← putative peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,112,0400TC100.0% 632.3 / NA 200A110A (GCA→GCG) b2972putative peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/102);  total (98/102)

Only 100 of 215 total aligned reads displayed.

ACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACA  >  NC_000913/3111950‑3112128
                                                                                          |                                                                                        
ccTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAgcgc                                                                                        <  1:1792178/92‑1 (MQ=255)
acccgcgGCAATCAGCCAGGCGGAAAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAACAAgcgc                                                                                        >  2:4546105/4‑93 (MQ=255)
acTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAgcgc                                                                                        >  1:3570896/1‑93 (MQ=255)
acTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAgcgc                                                                                        <  2:2741224/93‑1 (MQ=255)
   cgcgGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGt                                                                                     <  1:3893062/93‑1 (MQ=255)
   cgcgGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGC‑AAAAAGCAAGCGCTGTc                                                                                    <  2:4202040/93‑1 (MQ=255)
     cgacAATCAGCCAGCCGGAAAATATCATCACCCCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCa                                                                                   <  2:2046992/90‑1 (MQ=255)
      ggCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCaa                                                                                  >  2:3825342/1‑93 (MQ=255)
       gCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAAt                                                                                 >  2:3370715/1‑93 (MQ=255)
         aaTCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATaa                                                                               >  2:4646872/1‑93 (MQ=255)
             aGCCAGGCGGATAATATCATCACCGCCACCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTcc                                                                           >  1:2967510/1‑93 (MQ=255)
              gCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTcca                                                                          >  1:4537977/1‑93 (MQ=255)
              gCCAGCCGGATAATATCATCCCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTcca                                                                          <  2:1552590/93‑1 (MQ=255)
                      gATAATATCATCACGGCCAGCCCCCATCCTCTTTCTGGCCAGACCAGACTCGCCAGCAAAAAACCAAGCGCTGTCAATAACTCCACCAGCGGa                                                                  <  2:4283936/93‑1 (MQ=255)
                      gATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGa                                                                  >  1:1249359/1‑93 (MQ=255)
                       ataataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGAt                                                                 >  2:2817499/1‑93 (MQ=255)
                        taataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATa                                                                >  1:1676804/1‑93 (MQ=255)
                        taataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATa                                                                <  1:2416259/93‑1 (MQ=255)
                        taataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATa                                                                >  1:933034/1‑93 (MQ=255)
                         aataTCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATaa                                                               <  1:4241127/93‑1 (MQ=255)
                          atatCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAAc                                                              >  1:2008729/1‑93 (MQ=255)
                          atatCACCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAAc                                                              <  2:4385170/93‑1 (MQ=255)
                            accatccCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGc                                                            <  2:1466094/91‑1 (MQ=255)
                             tcatcaCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCt                                                           >  1:3771527/1‑93 (MQ=255)
                              catcaCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCtt                                                          <  2:1160245/93‑1 (MQ=255)
                              catcaCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCtt                                                          <  1:787848/93‑1 (MQ=255)
                              catcaCCGCCACCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCtt                                                          <  1:2256285/93‑1 (MQ=255)
                                ccacccccaccccCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGc                                                        <  1:2743077/92‑1 (MQ=255)
                                 caCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCt                                                       <  2:328210/93‑1 (MQ=255)
                                   ccGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGa                                                     <  1:3399867/93‑1 (MQ=255)
                                       cAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAtttt                                                 <  1:130096/93‑1 (MQ=255)
                                        cgCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTc                                                >  2:3141729/2‑93 (MQ=255)
                                        aGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTc                                                >  1:3625806/1‑93 (MQ=255)
                                        aGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTc                                                <  1:909995/93‑1 (MQ=255)
                                        aGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTc                                                >  1:4109988/1‑93 (MQ=255)
                                          cccccATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGc                                              >  1:4439796/1‑93 (MQ=255)
                                           ccccATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGcc                                             <  1:3181073/93‑1 (MQ=255)
                                             ccATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTg                                           >  1:549509/1‑93 (MQ=255)
                                              cATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGa                                          <  1:2920975/93‑1 (MQ=255)
                                              cATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGa                                          >  1:1422897/1‑93 (MQ=255)
                                              cATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGa                                          >  2:220102/1‑93 (MQ=255)
                                               aTCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAc                                         >  1:521879/1‑93 (MQ=255)
                                               aTCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAc                                         >  2:2076879/1‑93 (MQ=255)
                                               aTCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAc                                         <  1:2609676/93‑1 (MQ=255)
                                               aTCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAc                                         >  1:2817387/1‑93 (MQ=255)
                                                tCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACa                                        >  1:4257660/1‑93 (MQ=255)
                                                 ccACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAg                                       >  1:3996563/1‑93 (MQ=255)
                                                 ccACTTTCGGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAg                                       <  1:1544799/93‑1 (MQ=255)
                                                  cACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGt                                      >  1:1452984/1‑93 (MQ=255)
                                                  cACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGt                                      >  2:441171/1‑93 (MQ=255)
                                                  cACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGt                                      >  2:3496553/1‑93 (MQ=255)
                                                  cACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGt                                      <  1:1110317/93‑1 (MQ=255)
                                                    cTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCCATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTcg                                    >  2:3778998/1‑93 (MQ=255)
                                                    cTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATGACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTcg                                    >  1:1162744/1‑93 (MQ=255)
                                                    cTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTcg                                    <  1:3020744/93‑1 (MQ=255)
                                                     tttCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTcgc                                   <  2:664649/93‑1 (MQ=255)
                                                       tCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCgg                                 <  2:1624447/93‑1 (MQ=255)
                                                       tCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCgg                                 <  2:1692612/93‑1 (MQ=255)
                                                        cTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGc                                <  2:403249/93‑1 (MQ=255)
                                                        cTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGc                                <  2:3682972/93‑1 (MQ=255)
                                                         tGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCa                               <  1:514301/93‑1 (MQ=255)
                                                            ccagaccagaCTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAgcg                            <  1:2304856/93‑1 (MQ=255)
                                                               gaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGccc                         >  2:4494600/1‑93 (MQ=255)
                                                               gaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGccc                         >  2:2955206/1‑93 (MQ=255)
                                                               gaccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGccc                         >  2:2287893/1‑93 (MQ=255)
                                                                accagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCt                        <  2:1495580/93‑1 (MQ=255)
                                                                 ccagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCtt                       <  1:556787/93‑1 (MQ=255)
                                                                  cagacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCttt                      <  2:834010/93‑1 (MQ=255)
                                                                   agacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTg                     >  1:1651828/1‑93 (MQ=255)
                                                                    gacTCGCCAGCAAAAAAGCAAGCGCTGTCAATATCTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGa                    <  2:1736369/93‑1 (MQ=255)
                                                                    gacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGa                    <  2:2610164/93‑1 (MQ=255)
                                                                    gacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGa                    >  2:4146965/1‑93 (MQ=255)
                                                                    gacTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGa                    >  1:3275969/1‑93 (MQ=255)
                                                                      cTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGc                  >  2:3950070/1‑93 (MQ=255)
                                                                       tCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCa                 >  2:761185/1‑93 (MQ=255)
                                                                        cGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCAt                >  1:1241789/1‑93 (MQ=255)
                                                                         gCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATc               <  2:598064/93‑1 (MQ=255)
                                                                         gCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATc               <  1:70853/93‑1 (MQ=255)
                                                                         gCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATc               >  1:1568805/1‑93 (MQ=255)
                                                                         gCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATc               <  2:1034527/93‑1 (MQ=255)
                                                                         gCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCAGCAGCGCCCTTTGAGCATc               <  2:4579073/93‑1 (MQ=255)
                                                                          ccAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCa              >  1:3628001/1‑93 (MQ=255)
                                                                           cAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAg             >  2:3700902/1‑93 (MQ=255)
                                                                            aGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGc            >  1:2565107/1‑93 (MQ=255)
                                                                            aGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGc            >  2:2302794/1‑93 (MQ=255)
                                                                             gCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGcc           >  2:3588957/1‑93 (MQ=255)
                                                                              cAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCa          <  2:955001/93‑1 (MQ=255)
                                                                              cAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCa          >  1:3221572/1‑93 (MQ=255)
                                                                              cAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCa          >  2:3058080/1‑93 (MQ=255)
                                                                               aaaaaaGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAg         >  2:1913635/1‑93 (MQ=255)
                                                                               aaaaaaGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAg         <  2:2373388/93‑1 (MQ=255)
                                                                                 aaaaGCAAGCGCTGTCAATATCTTCACCAGCGGTTAACGGTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGga       <  2:4442963/93‑1 (MQ=255)
                                                                                   aggCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGgaga     <  1:3877615/91‑1 (MQ=255)
                                                                                   aagcaagcGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGgaga     >  1:3711068/1‑93 (MQ=255)
                                                                                    aGCAAGCTCTCTCATTACCTCCACCAGCGGATACCGCTTGCTGATTTTCGCCTGACAGTCGGGGCAGCGCCCTTTGAGCATCAGCCAGGAGaa    <  2:1265204/93‑1 (MQ=255)
                                                                                    aGCAAGCGCTGTCAATACCTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGaa    <  1:645546/93‑1 (MQ=255)
                                                                                    aGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGaa    <  1:1722447/93‑1 (MQ=255)
                                                                                     gCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAc   >  1:1852326/1‑93 (MQ=255)
                                                                                     gCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAc   <  2:4117458/93‑1 (MQ=255)
                                                                                      cAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACa  <  2:585457/93‑1 (MQ=255)
                                                                                          |                                                                                        
ACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACA  >  NC_000913/3111950‑3112128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACA  >  NC_000913/3111949‑3112128
                                                                                           |                                                                                        
CACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGC                                                                                        >  SRR4302535.1150996/1‑94 (MQ=60)
CACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGC                                                                                        >  SRR4302535.3595660/1‑94 (MQ=60)
 CCTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCT                                                                                       <  SRR4302535.1805135/94‑1 (MQ=60)
 ACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCT                                                                                       >  SRR4302535.1000621/1‑94 (MQ=60)
 ACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCT                                                                                       >  SRR4302535.245018/1‑94 (MQ=60)
    CGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTC                                                                                    <  SRR4302535.3919749/94‑1 (MQ=60)
    CGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTC                                                                                    >  SRR4302535.2688265/1‑94 (MQ=60)
     GCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCA                                                                                   <  SRR4302535.3837375/94‑1 (MQ=60)
       GGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAAT                                                                                 <  SRR4302535.2730963/94‑1 (MQ=60)
        GCAATCAGGCAGGCGGAAAATTACATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATA                                                                                <  SRR4302535.3661326/94‑1 (MQ=60)
         CAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAA                                                                               <  SRR4302535.1566583/94‑1 (MQ=60)
         CAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAA                                                                               >  SRR4302535.1827290/1‑94 (MQ=60)
          AATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAAC                                                                              >  SRR4302535.2344228/1‑94 (MQ=60)
          AATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAAC                                                                              <  SRR4302535.2995436/94‑1 (MQ=60)
            TCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTC                                                                            >  SRR4302535.4274938/1‑94 (MQ=60)
             CAGCCAGGCGGATAATATCATCACCGCCACCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCC                                                                           >  SRR4302535.2988333/1‑94 (MQ=60)
              AGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCA                                                                          >  SRR4302535.1387068/1‑94 (MQ=60)
              AGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCA                                                                          >  SRR4302535.4569021/1‑94 (MQ=60)
                CCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACC                                                                        <  SRR4302535.3944714/94‑1 (MQ=60)
                CCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACC                                                                        <  SRR4302535.325145/94‑1 (MQ=60)
                CCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACC                                                                        >  SRR4302535.3244122/1‑94 (MQ=60)
                CCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACC                                                                        >  SRR4302535.125376/1‑94 (MQ=60)
                  AGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAG                                                                      <  SRR4302535.932818/94‑1 (MQ=60)
                  AGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAG                                                                      >  SRR4302535.617767/1‑94 (MQ=60)
                   GGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGC                                                                     <  SRR4302535.3194093/94‑1 (MQ=60)
                      GGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGA                                                                  >  SRR4302535.1258275/1‑94 (MQ=60)
                      GGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGA                                                                  >  SRR4302535.1481150/1‑94 (MQ=60)
                        ATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATA                                                                >  SRR4302535.1688948/1‑94 (MQ=60)
                        ATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATA                                                                >  SRR4302535.939634/1‑94 (MQ=60)
                        ATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATA                                                                >  SRR4302535.3899546/1‑94 (MQ=60)
                         TAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAA                                                               <  SRR4302535.2433651/94‑1 (MQ=60)
                          AATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAAC                                                              >  SRR4302535.2023195/1‑94 (MQ=60)
                          AATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAAC                                                              <  SRR4302535.4270156/94‑1 (MQ=60)
                           ATATCACCCCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACG                                                             <  SRR4302535.231382/94‑1 (MQ=60)
                            TATCATCCCCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGC                                                            <  SRR4302535.1306372/94‑1 (MQ=60)
                             ATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCT                                                           >  SRR4302535.3797547/1‑94 (MQ=60)
                               CATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTG                                                         <  SRR4302535.1792489/94‑1 (MQ=60)
                               CATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTG                                                         <  SRR4302535.793378/94‑1 (MQ=60)
                               CATCACCGCCACCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTG                                                         <  SRR4302535.2272348/94‑1 (MQ=60)
                                ATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGC                                                        <  SRR4302535.2168667/94‑1 (MQ=60)
                                ATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGC                                                        >  SRR4302535.4295911/1‑94 (MQ=60)
                                 ccacccccacCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCT                                                       <  SRR4302535.2762629/84‑1 (MQ=60)
                                   ACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGA                                                     >  SRR4302535.3564358/1‑94 (MQ=60)
                                    CCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGAT                                                    <  SRR4302535.3423529/94‑1 (MQ=60)
                                      GCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTT                                                  <  SRR4302535.4243478/94‑1 (MQ=60)
                                      CCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTT                                                  <  SRR4302535.2837104/94‑1 (MQ=60)
                                        CAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTC                                                >  SRR4302535.3650935/1‑94 (MQ=60)
                                        CAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTC                                                >  SRR4302535.3650934/1‑94 (MQ=60)
                                        CAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTC                                                <  SRR4302535.131011/94‑1 (MQ=60)
                                        CAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTC                                                >  SRR4302535.4138125/1‑94 (MQ=60)
                                        CAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTC                                                <  SRR4302535.521436/94‑1 (MQ=60)
                                         AGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCG                                               <  SRR4302535.801521/94‑1 (MQ=60)
                                         AGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCG                                               <  SRR4302535.916463/94‑1 (MQ=60)
                                          GCCCCCATCCCCTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGC                                              <  SRR4302535.548546/94‑1 (MQ=60)
                                          GCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGC                                              >  SRR4302535.4470231/1‑94 (MQ=60)
                                            CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCT                                            >  SRR4302535.3793687/1‑94 (MQ=60)
                                            CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCT                                            <  SRR4302535.3203061/94‑1 (MQ=60)
                                            CCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCT                                            >  SRR4302535.1920102/1‑94 (MQ=60)
                                             CCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTG                                           >  SRR4302535.553351/1‑94 (MQ=60)
                                             CCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTG                                           >  SRR4302535.2612904/1‑94 (MQ=60)
                                              CCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGA                                          >  SRR4302535.1433056/1‑94 (MQ=60)
                                               CATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAC                                         >  SRR4302535.2837397/1‑94 (MQ=60)
                                               CATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAC                                         >  SRR4302535.487389/1‑94 (MQ=60)
                                               CATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAC                                         <  SRR4302535.148852/94‑1 (MQ=60)
                                               CATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAC                                         >  SRR4302535.525530/1‑94 (MQ=60)
                                               CATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGAC                                         <  SRR4302535.2941582/94‑1 (MQ=60)
                                                ATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACA                                        >  SRR4302535.3601629/1‑94 (MQ=60)
                                                ATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACA                                        <  SRR4302535.2628403/94‑1 (MQ=60)
                                                ATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACA                                        >  SRR4302535.4286828/1‑94 (MQ=60)
                                                 TCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAG                                       >  SRR4302535.4023906/1‑94 (MQ=60)
                                                  CCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGT                                      >  SRR4302535.1463384/1‑94 (MQ=60)
                                                  CCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGT                                      >  SRR4302535.676183/1‑94 (MQ=60)
                                                  CCACTTTCGGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGT                                      <  SRR4302535.1555925/94‑1 (MQ=60)
                                                   CACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTC                                     <  SRR4302535.2953214/94‑1 (MQ=60)
                                                   CACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTC                                     <  SRR4302535.1118245/94‑1 (MQ=60)
                                                    CCTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCG                                    <  SRR4302535.3218667/94‑1 (MQ=60)
                                                    ACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATGACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCG                                    >  SRR4302535.1171053/1‑94 (MQ=60)
                                                     CTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGC                                   <  SRR4302535.1195346/94‑1 (MQ=60)
                                                     CTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGC                                   <  SRR4302535.3041898/94‑1 (MQ=60)
                                                         CTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCA                               >  SRR4302535.76584/1‑94 (MQ=60)
                                                          TGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAG                              <  SRR4302535.517895/94‑1 (MQ=60)
                                                             CCAGACCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGC                           <  SRR4302535.2321343/94‑1 (MQ=60)
                                                                  CCAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTT                      <  SRR4302535.560689/94‑1 (MQ=60)
                                                                   CAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTG                     >  SRR4302535.1452271/1‑94 (MQ=60)
                                                                   CAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTG                     >  SRR4302535.1663774/1‑94 (MQ=60)
                                                                   CAGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTG                     <  SRR4302535.1657268/94‑1 (MQ=60)
                                                                    AGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGA                    >  SRR4302535.4059534/1‑94 (MQ=60)
                                                                    AGACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGA                    >  SRR4302535.3298724/1‑94 (MQ=60)
                                                                     GACTCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAG                   <  SRR4302535.558275/94‑1 (MQ=60)
                                                                        TCGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCAT                >  SRR4302535.1250659/1‑94 (MQ=60)
                                                                         CGCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATC               >  SRR4302535.1580128/1‑94 (MQ=60)
                                                                          GCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCA              >  SRR4302535.3653146/1‑94 (MQ=60)
                                                                          GCCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCA              <  SRR4302535.71372/94‑1 (MQ=60)
                                                                           CCAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAG             <  SRR4302535.1712552/94‑1 (MQ=60)
                                                                            CAGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGC            >  SRR4302535.2583567/1‑94 (MQ=60)
                                                                             AGCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCC           >  SRR4302535.4376492/1‑94 (MQ=60)
                                                                              GCAAAAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCA          >  SRR4302535.3243896/1‑94 (MQ=60)
                                                                                  AAAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAG      >  SRR4302535.1972898/1‑94 (MQ=60)
                                                                                   AAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGA     >  SRR4302535.3736775/1‑94 (MQ=60)
                                                                                   AAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGA     <  SRR4302535.288137/94‑1 (MQ=60)
                                                                                   AAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGA     >  SRR4302535.3907543/1‑94 (MQ=60)
                                                                                   AAAGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGA     <  SRR4302535.4518106/94‑1 (MQ=60)
                                                                                    AGGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAA    <  SRR4302535.3904244/94‑1 (MQ=60)
                                                                                     AGCAAGCGCTGTCAATACCTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAC   <  SRR4302535.650051/94‑1 (MQ=60)
                                                                                     AGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAC   >  SRR4302535.688824/1‑94 (MQ=60)
                                                                                     AGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAC   <  SRR4302535.1734904/94‑1 (MQ=60)
                                                                                     AGCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAAC   >  SRR4302535.1865671/1‑94 (MQ=60)
                                                                                      GCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCCGCCAGGAGAACA  >  SRR4302535.1672433/1‑94 (MQ=60)
                                                                                      GCAAGCGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACA  <  SRR4302535.339884/94‑1 (MQ=60)
                                                                                           |                                                                                        
CACTCGCGGCAATCAGCCAGGCGGATAATATCATCACCGCCAGCCCCCATCCACTTTCTGGCCAGACCAGACTCGCCAGCAAAAAAGCAAGTGCTGTCAATAACTCCACCAGCGGATAACGCTTGCTGATTTTCGCCTGACAGTCGCGGCAGCGCCCTTTGAGCATCAGCCAGGAGAACA  >  NC_000913/3111949‑3112128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑