Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,117,133 A→G intergenic (‑5/+480) b2974 ← / ← yghK putative endoglucanase/putative permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,117,1330AG100.0% 243.0 / NA 78intergenic (‑5/+480)b2974/yghKputative endoglucanase/putative permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/34);  total (44/34)

CCACCGCCATCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATAACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTA  >  NC_000913/3117046‑3117221
                                                                                       |                                                                                        
ccaccGCCATCACACCCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTGAAATTTCTTATTCATTAATGACGCa                                                                                     <  2:456574/93‑1 (MQ=255)
  cccGCCATCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAg                                                                                   <  2:961339/92‑1 (MQ=255)
   ccGCCATCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGt                                                                                  <  2:1320961/93‑1 (MQ=255)
   ccGCCATCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGt                                                                                  >  2:2547489/1‑93 (MQ=255)
        aTCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACaa                                                                             >  2:183648/1‑93 (MQ=255)
        aTCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACaa                                                                             <  1:2036486/93‑1 (MQ=255)
         tCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACaaa                                                                            <  1:1999333/93‑1 (MQ=255)
          cacaACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACaaaa                                                                           <  1:3802490/93‑1 (MQ=255)
           acaACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAAc                                                                          <  2:945984/93‑1 (MQ=255)
             aaCCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACaa                                                                        <  2:1403909/93‑1 (MQ=255)
             aaCCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACaa                                                                        <  2:4353927/93‑1 (MQ=255)
             aaCCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACaa                                                                        <  2:4479757/93‑1 (MQ=255)
              aCCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAAt                                                                       <  1:2565476/93‑1 (MQ=255)
              aCCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAAt                                                                       <  2:4308608/93‑1 (MQ=255)
               ccGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATg                                                                      <  1:2377678/93‑1 (MQ=255)
               ccGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATg                                                                      >  1:606288/1‑93 (MQ=255)
                cGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGt                                                                     <  1:2183439/93‑1 (MQ=255)
                          ggTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTc                                                           <  2:4528741/93‑1 (MQ=255)
                           gTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCg                                                          >  1:2033521/1‑93 (MQ=255)
                           gTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCg                                                          >  1:3191686/1‑93 (MQ=255)
                           gTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCg                                                          <  1:4206836/93‑1 (MQ=255)
                                    aaaaTAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGga                                                 <  2:759411/93‑1 (MQ=255)
                                       aTAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGaa                                              >  1:3891458/1‑93 (MQ=255)
                                           ccGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAg                                          <  2:2608983/93‑1 (MQ=255)
                                             gCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGtt                                        >  1:2305328/1‑93 (MQ=255)
                                               tAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGtttt                                      <  1:915668/93‑1 (MQ=255)
                                               tAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGtttt                                      <  1:3043539/93‑1 (MQ=255)
                                                 aaaGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTAt                                    <  2:693826/93‑1 (MQ=255)
                                                      gATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCttt                               >  1:3790169/1‑93 (MQ=255)
                                                       aTTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTa                              <  2:393258/93‑1 (MQ=255)
                                                       aTTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTa                              >  2:724087/1‑93 (MQ=255)
                                                        tttCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAt                             >  1:4342953/1‑93 (MQ=255)
                                                        tttCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAt                             >  1:1099802/1‑93 (MQ=255)
                                                          tCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAtct                           >  1:1475401/1‑93 (MQ=255)
                                                           cTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGATATAGTTTTATGCTTTAtctt                          >  2:3857761/1‑93 (MQ=255)
                                                            ttATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAtcttc                         >  1:3242372/1‑93 (MQ=255)
                                                            ttATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAtcttc                         <  2:3273413/93‑1 (MQ=255)
                                                             tataTTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAtcttct                        >  2:4205368/1‑93 (MQ=255)
                                                             tataTTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAtcttct                        >  2:2915752/1‑93 (MQ=255)
                                                             tataTTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAtcttct                        >  1:4634382/1‑93 (MQ=255)
                                                             tataTTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAtcttct                        >  1:2736922/1‑93 (MQ=255)
                                                             tataTTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAtcttct                        >  1:2646142/1‑93 (MQ=255)
                                                             tataTTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAtcttct                        >  2:177440/1‑93 (MQ=255)
                                                              atatTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTtctc                       <  2:3605573/93‑1 (MQ=255)
                                                              atatTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTtctc                       <  2:3968434/93‑1 (MQ=255)
                                                                atTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCta                     >  2:4400102/1‑93 (MQ=255)
                                                                atTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCta                     >  1:799436/1‑93 (MQ=255)
                                                                atTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCta                     >  1:663646/1‑93 (MQ=255)
                                                                 tttAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGCAATAGTTTTATGCTTTATCTTctctca                    >  1:1935241/1‑91 (MQ=255)
                                                                 tttAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCtaa                    >  1:3205833/1‑93 (MQ=255)
                                                                  ttAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCtaat                   <  1:3023661/93‑1 (MQ=255)
                                                                  ttAAATTTCTTATTCAGTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCtaat                   <  2:1133828/93‑1 (MQ=255)
                                                                   tAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCtaata                  <  1:3348583/93‑1 (MQ=255)
                                                                   tAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCtaata                  >  1:2785654/1‑93 (MQ=255)
                                                                   tAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGGTTTATGCTTTATCTTCTCttata                  >  2:2885165/1‑93 (MQ=255)
                                                                    aaaTTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCtaataa                 >  1:4359775/1‑93 (MQ=255)
                                                                     aaTTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAAc                <  2:2804002/93‑1 (MQ=255)
                                                                     aaTTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAAc                <  2:1548464/93‑1 (MQ=255)
                                                                      aTTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACt               >  1:454731/1‑93 (MQ=255)
                                                                      aTTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACt               >  1:4220744/1‑93 (MQ=255)
                                                                       tttCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACtt              >  2:2255133/1‑93 (MQ=255)
                                                                        ttCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTtc             >  2:1206771/1‑93 (MQ=255)
                                                                         tCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTtcc            >  1:1900943/1‑93 (MQ=255)
                                                                          cTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTtcct           >  1:2662854/1‑93 (MQ=255)
                                                                            tATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTtcctcc         >  1:2600619/1‑93 (MQ=255)
                                                                            tATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTtcctcc         <  1:507366/93‑1 (MQ=255)
                                                                             aTTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCa        <  1:1107975/93‑1 (MQ=255)
                                                                             aTTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCa        <  1:4124705/93‑1 (MQ=255)
                                                                             aTTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCa        >  2:2635806/1‑93 (MQ=255)
                                                                              ttCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAt       <  2:2841866/93‑1 (MQ=255)
                                                                              ttCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAt       >  2:489697/1‑93 (MQ=255)
                                                                              ttCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAt       >  2:834700/1‑93 (MQ=255)
                                                                              ttCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAt       >  2:1293038/1‑93 (MQ=255)
                                                                               tCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAtt      >  1:446668/1‑93 (MQ=255)
                                                                               tCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAtt      >  2:358618/1‑93 (MQ=255)
                                                                               tCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAtt      >  2:4512906/1‑93 (MQ=255)
                                                                               tCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAtt      >  2:3122575/1‑93 (MQ=255)
                                                                                 aTTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCt    >  1:693569/1‑93 (MQ=255)
                                                                                   taatgaCGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTa  >  1:3486319/1‑93 (MQ=255)
                                                                                   taatgaCGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTa  >  2:2142943/1‑93 (MQ=255)
                                                                                   taatgaCGCAAGTGACAAAACAATGTTAAAAAAGTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTa  <  1:2361187/93‑1 (MQ=255)
                                                                                       |                                                                                        
CCACCGCCATCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATAACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTA  >  NC_000913/3117046‑3117221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATAACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGA  >  NC_000913/3117054‑3117224
                                                                               |                                                                                           
ATCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAA                                                                               <  SRR4302535.2051153/94‑1 (MQ=60)
 TCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAA                                                                              <  SRR4302535.2013735/94‑1 (MQ=60)
  CACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAAC                                                                             <  SRR4302535.3828702/94‑1 (MQ=60)
      ACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATG                                                                         <  SRR4302535.2583937/94‑1 (MQ=60)
      ACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATG                                                                         >  SRR4302535.610524/1‑94 (MQ=60)
       CCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGT                                                                        <  SRR4302535.2394756/94‑1 (MQ=60)
        CGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTT                                                                       <  SRR4302535.2199008/94‑1 (MQ=60)
                  GGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCG                                                             >  SRR4302535.3213763/1‑94 (MQ=60)
                  GGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCG                                                             >  SRR4302535.2048174/1‑94 (MQ=60)
                   GTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGT                                                            <  SRR4302535.4235626/94‑1 (MQ=60)
                              AATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAA                                                 >  SRR4302535.3918140/1‑94 (MQ=60)
                                    CGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTT                                           >  SRR4302535.2321819/1‑94 (MQ=60)
                                       TAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTA                                        <  SRR4302535.922169/94‑1 (MQ=60)
                                       TAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTA                                        <  SRR4302535.3064862/94‑1 (MQ=60)
                                             CGATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTT                                  >  SRR4302535.3816306/1‑94 (MQ=60)
                                               ATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAT                                >  SRR4302535.4372651/1‑94 (MQ=60)
                                               ATTTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTAT                                >  SRR4302535.1107657/1‑94 (MQ=60)
                                                 TTCTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCT                              >  SRR4302535.1485965/1‑94 (MQ=60)
                                                   CTTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTC                            >  SRR4302535.3264894/1‑94 (MQ=60)
                                                    TTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCT                           >  SRR4302535.2665093/1‑94 (MQ=60)
                                                    TTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCT                           >  SRR4302535.4665819/1‑94 (MQ=60)
                                                    TTATATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCT                           >  SRR4302535.2756444/1‑94 (MQ=60)
                                                       TATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTA                        >  SRR4302535.668283/1‑94 (MQ=60)
                                                       TATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTA                        >  SRR4302535.805048/1‑94 (MQ=60)
                                                        ATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGCAATAGTTTTATGCTTTATCTTCTCTCA                       >  SRR4302535.1949179/1‑94 (MQ=60)
                                                        ATTTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAA                       >  SRR4302535.3228034/1‑94 (MQ=60)
                                                          TTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATA                     <  SRR4302535.3044831/94‑1 (MQ=60)
                                                          TTAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATA                     >  SRR4302535.2805478/1‑94 (MQ=60)
                                                           TAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAA                    <  SRR4302535.3371910/94‑1 (MQ=60)
                                                           TAAATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAA                    >  SRR4302535.4389616/1‑94 (MQ=60)
                                                             AATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACT                  >  SRR4302535.457916/1‑94 (MQ=60)
                                                             AATTTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACT                  >  SRR4302535.4249626/1‑94 (MQ=60)
                                                                TTCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCC               >  SRR4302535.1914623/1‑94 (MQ=60)
                                                                 TCTTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCT              >  SRR4302535.2681923/1‑94 (MQ=60)
                                                                   TTATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCC            >  SRR4302535.2619287/1‑94 (MQ=60)
                                                                    TATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCA           <  SRR4302535.510913/94‑1 (MQ=60)
                                                                     ATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAT          <  SRR4302535.4152933/94‑1 (MQ=60)
                                                                     ATTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCAT          <  SRR4302535.1115887/94‑1 (MQ=60)
                                                                      TTCATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATT         >  SRR4302535.449794/1‑94 (MQ=60)
                                                                        CATTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCT       >  SRR4302535.698433/1‑94 (MQ=60)
                                                                          TTAATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTA     >  SRR4302535.3510523/1‑94 (MQ=60)
                                                                           TAATGACGCAAGTGACAAAACAATGTTAAAAAAGTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAA    <  SRR4302535.2378139/94‑1 (MQ=60)
                                                                             ATGACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGA  <  SRR4302535.593771/94‑1 (MQ=60)
                                                                               |                                                                                           
ATCACAACCGGCTAACAGGGTTGCGCTCAAAATAGCCGCTAAAAGCGATTTCTTATATTTAAATTTCTTATTCATTAATAACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGA  >  NC_000913/3117054‑3117224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑