Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,130,334 A→G V30V (GTT→GTC)  b2983 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,130,3340AG100.0% 554.6 / NA 174V30V (GTT→GTC) b2983hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (66/108);  total (66/108)

Only 100 of 190 total aligned reads displayed.

TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA  >  NC_000913/3130242‑3130423
                                                                                            |                                                                                         
tACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCCGCACACGAGAGCGCCCACAGACCCCGCCACGCGCTcaca                                                                                           >  1:4368002/1‑93 (MQ=255)
   gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCCTCCCTTTACCGGCACACGAGAGAGCCAACAAAACCAGCAAGGCGCTCacgaca                                                                                        >  1:3794952/1‑93 (MQ=255)
   gATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAAAAGACCCAGCAAGGAGCTCacgaca                                                                                        >  1:2231386/1‑93 (MQ=255)
    aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAt                                                                                       <  2:681429/93‑1 (MQ=255)
    aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAt                                                                                       >  1:364165/1‑93 (MQ=255)
    aTTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCCTTAACGGCACACGAGAGCGCCAACAGACCCAGCCAGGCGCTCACCACAt                                                                                       >  2:2428376/1‑93 (MQ=255)
       gCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGGCAAAAGAACCAACAAGGCGCCCACGACAATAc                                                                                    >  2:1992785/1‑93 (MQ=255)
        cacaATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACt                                                                                   <  1:572340/93‑1 (MQ=255)
        cacaACCACCAGTACGCGAAACATGCCGGGCGTCATCCCTTTCCCGGCACACGAGGGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACt                                                                                   <  2:2799749/93‑1 (MQ=255)
         acaATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTg                                                                                  <  1:388447/93‑1 (MQ=255)
          caAGCACCAGTACGCCAAACTGGGCGGGCATCCCCCTTCCGCCGGCACGGGGGGGCGCCACCAGACCAAGGGGGGCGCTCACGACATTACTgg                                                                                 <  2:933287/93‑1 (MQ=255)
           aaTCACCAGTCCGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGa                                                                                <  1:4388700/93‑1 (MQ=255)
            aTCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAg                                                                               >  2:3216001/1‑93 (MQ=255)
             tCACCAGTACGCCAAACATGGCGGGCGGCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGc                                                                              >  1:2834130/1‑93 (MQ=255)
              ccccAGTACGCCAAACAGGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAACGCGCTCACGACATTACTGGAGcc                                                                             <  2:1793763/91‑1 (MQ=255)
              cACTAGTACGCCAAAAATGGCGGGCGGCGTCCCTTTACCGGCACACGAGAGCGCCAACAGACCGAGAAAGGCGCTCACGACATTACTGGAGcc                                                                             <  2:3804271/93‑1 (MQ=255)
                ccAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTa                                                                           >  1:3127294/1‑93 (MQ=255)
                 cAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAGCAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTaa                                                                          <  2:726937/93‑1 (MQ=255)
                  aGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAc                                                                         <  1:522611/93‑1 (MQ=255)
                   gaaCGCAAAACAGGGGGGGCGCCACCCCTTACCGGGCACACGAGAGCCCCAACACCCCCAGCAGGCCCCTCACGACATTACTGGAGCCTAAcc                                                                        <  2:1928375/91‑1 (MQ=255)
                   gTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAcc                                                                        >  1:2717910/1‑93 (MQ=255)
                   gTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAcc                                                                        <  1:3073865/93‑1 (MQ=255)
                   gTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAcc                                                                        <  1:1446028/93‑1 (MQ=255)
                      cGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGTCTAACCAgg                                                                     >  2:3764568/1‑93 (MQ=255)
                       gCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgc                                                                    >  2:4632943/1‑93 (MQ=255)
                       gCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGgc                                                                    <  2:3113289/93‑1 (MQ=255)
                        ccAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCAAGACATTACTGGGGCCTAACCAGgcg                                                                   >  1:4115251/1‑93 (MQ=255)
                          aaaCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAACAGGCGCt                                                                 >  2:8380/1‑93 (MQ=255)
                          aaaCAGGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCt                                                                 <  1:138331/93‑1 (MQ=255)
                           acaaTGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTa                                                                <  1:3615794/90‑1 (MQ=255)
                           aaCATGGCGGGGGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTa                                                                >  2:2034332/1‑93 (MQ=255)
                           aaCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGAGCTCACGACATTACTGGAGCCTAACCAGGCGCTa                                                                >  1:3560192/1‑93 (MQ=255)
                           aaCAT‑GCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCCAt                                                               >  1:4287868/1‑93 (MQ=255)
                            acgtGGCGGGCTTCACCCCTTCCCCGGAACACGAGACCGCCAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAt                                                               <  2:1666424/90‑1 (MQ=255)
                            aCATGGCTGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAt                                                               <  2:1568525/93‑1 (MQ=255)
                            aCATGGCGGGGGTCATCCCTTTACCGGCACCGGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAt                                                               <  2:3570351/93‑1 (MQ=255)
                            aCATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAt                                                               >  2:4655901/1‑93 (MQ=255)
                             cagggcgggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAtt                                                              <  1:4500966/90‑1 (MQ=255)
                             cATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAtt                                                              >  1:1872216/1‑93 (MQ=255)
                               tGGCGGCGGTCACCCCTTTACCGCCACAGGAGAGCGCCAACAGCCCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGc                                                            <  2:2676180/93‑1 (MQ=255)
                                  cgggcgTCATCCCTTTACCGGCACACGAGAGCGCAAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaa                                                         <  2:295639/93‑1 (MQ=255)
                                   gggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaat                                                        >  2:1432066/1‑93 (MQ=255)
                                   gggcgTCACCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaat                                                        <  1:1944839/93‑1 (MQ=255)
                                    ggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGTCATTACTGGAGCCTAACCAGGCGCTATTGCGaata                                                       >  2:4685987/1‑93 (MQ=255)
                                    ggcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaata                                                       >  2:3831510/1‑93 (MQ=255)
                                     gcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                      <  2:3100150/93‑1 (MQ=255)
                                     gcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                      >  1:1299433/1‑93 (MQ=255)
                                     gcgTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataa                                                      <  2:542993/93‑1 (MQ=255)
                                      cttCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGaataat                                                     <  2:3640250/91‑1 (MQ=255)
                                        tCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATgg                                                   >  2:1827801/1‑93 (MQ=255)
                                         cATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                  <  1:1021573/93‑1 (MQ=255)
                                         cATCCCTTCACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGt                                                  <  1:4399106/93‑1 (MQ=255)
                                          aTCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTg                                                 >  1:3740793/1‑93 (MQ=255)
                                           tCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                                <  2:3020280/93‑1 (MQ=255)
                                           tCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                                <  2:4325483/93‑1 (MQ=255)
                                           tCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGc                                                <  1:3158048/93‑1 (MQ=255)
                                            cccTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCg                                               >  2:2935026/1‑93 (MQ=255)
                                              cTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGTGaa                                             >  2:466490/1‑93 (MQ=255)
                                               tttCCCGGACCAGGAGAGCGCCAACAGACCCAGCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                            <  1:1735645/93‑1 (MQ=255)
                                               tttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGaaa                                            <  2:1479046/93‑1 (MQ=255)
                                                ttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                           <  1:806145/93‑1 (MQ=255)
                                                ttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                           >  2:3356437/1‑93 (MQ=255)
                                                ttACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAc                                           <  2:3929056/93‑1 (MQ=255)
                                                 tACCGCCACAGGAGAGCGCCAACAGACCCAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcg                                          <  2:1090628/93‑1 (MQ=255)
                                                  aCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcgc                                         >  2:2194853/1‑93 (MQ=255)
                                                  aCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAcgc                                         <  2:642268/93‑1 (MQ=255)
                                                     ggCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGcc                                      <  1:2908837/93‑1 (MQ=255)
                                                      gcccacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCa                                     <  2:4322282/90‑1 (MQ=255)
                                                       cacaCGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATGATGGTGCGAATCGCGCCAt                                    <  2:4581936/93‑1 (MQ=255)
                                                        ccaggagaGCGCAAACACACCCAGCAGGCCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATc                                   <  2:1653624/89‑1 (MQ=255)
                                                        acacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATc                                   <  2:3107504/93‑1 (MQ=255)
                                                        acacGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATc                                   >  1:134917/1‑93 (MQ=255)
                                                          acGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTg                                 >  2:873852/1‑93 (MQ=255)
                                                          acGAGAGCGACAACACCCCCACCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTg                                 <  1:2530508/93‑1 (MQ=255)
                                                             agagCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCaa                              >  2:2642229/1‑93 (MQ=255)
                                                               agCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAAc                            >  1:2310517/1‑93 (MQ=255)
                                                                gcgcCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAAcc                           <  1:1461256/93‑1 (MQ=255)
                                                                 ccccAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCa                          <  2:2562334/91‑1 (MQ=255)
                                                                    cAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATg                       <  1:1131727/93‑1 (MQ=255)
                                                                    cAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATg                       >  1:2664334/1‑93 (MQ=255)
                                                                      aCAGACCCAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGtt                     <  2:3412386/93‑1 (MQ=255)
                                                                       cAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                    <  1:156010/93‑1 (MQ=255)
                                                                       cAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGttt                    <  1:2084351/93‑1 (MQ=255)
                                                                        aGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTg                   >  2:1210123/1‑93 (MQ=255)
                                                                         gACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  <  2:4551630/93‑1 (MQ=255)
                                                                         gACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  >  1:2014360/1‑93 (MQ=255)
                                                                         gACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGa                  >  2:1386566/1‑93 (MQ=255)
                                                                           cccAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATg                >  2:4636487/1‑93 (MQ=255)
                                                                                  aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc         <  1:3699383/93‑1 (MQ=255)
                                                                                  aGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAc         <  2:3690737/93‑1 (MQ=255)
                                                                                   ggggcTCACGACATTACTGGAGCCTAACCAGGGGCTATTTCGAATAATGGTGCGAAACGTGCCATCTGCAAACCAATGTTTGATGTTAAAAcc        >  2:2565417/4‑93 (MQ=255)
                                                                                   ggCTCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc        <  1:3212503/93‑1 (MQ=255)
                                                                                   ggCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAcc        <  2:870421/93‑1 (MQ=255)
                                                                                    gcgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAAccc       <  2:475019/93‑1 (MQ=255)
                                                                                     cgcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCg      <  2:2084406/93‑1 (MQ=255)
                                                                                      gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGc     >  1:1697104/1‑93 (MQ=255)
                                                                                      gcTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGc     >  1:1022564/1‑93 (MQ=255)
                                                                                        tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa   <  1:1331326/93‑1 (MQ=255)
                                                                                        tCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCa   >  1:76845/1‑93 (MQ=255)
                                                                                         cacgACATTACTGGAGCCTAACCAGCCTCTATTGCGAATAATGGTGCGAAACGCCCCATCTGCAACCCAATGTTTGATGTTAAAACCCGCCaa  <  2:2286277/93‑1 (MQ=255)
                                                                                            |                                                                                         
TACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA  >  NC_000913/3130242‑3130423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427
                                                                                             |                                                                                             
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCCGCACACGAGAGCGCCCACAGACCCCGCCACGCGCTCACA                                                                                               >  SRR4302535.4397892/1‑94 (MQ=60)
  ACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGAC                                                                                             <  SRR4302535.773812/94‑1 (MQ=60)
  ACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCAACCAGTAGCCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGAC                                                                                             <  SRR4302535.2887359/94‑1 (MQ=60)
   CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCCTCCCTTTACCGGCACACGAGAGAGCCAACAAAACCAGCAAGGCGCTCACGACA                                                                                            >  SRR4302535.3821111/1‑94 (MQ=60)
   CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACA                                                                                            >  SRR4302535.3562875/1‑94 (MQ=60)
   CGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAAAAGACCCAGCAAGGAGCTCACGACA                                                                                            >  SRR4302535.2247253/1‑94 (MQ=60)
    GATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACAT                                                                                           >  SRR4302535.366673/1‑94 (MQ=60)
         CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTG                                                                                      <  SRR4302535.2225651/94‑1 (MQ=60)
         CACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTG                                                                                      <  SRR4302535.576358/94‑1 (MQ=60)
          ACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGG                                                                                     <  SRR4302535.391146/94‑1 (MQ=60)
            AATCACTAGTACGCAAAACTTGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCAAACAGCCCAAGCAAGGCGCTCACGACATTACTGGAG                                                                                   <  SRR4302535.839559/94‑1 (MQ=60)
            AATCACCAGTCCGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAG                                                                                   <  SRR4302535.4418722/94‑1 (MQ=60)
             ATCACCAGTACGCCAAACATGGCGGGCGGCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGC                                                                                  >  SRR4302535.2854244/1‑94 (MQ=60)
               CACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCCGCACACGGGAGCGCCAACAGACCCAGCAAAGCGCTCACGACATTACTCGAGCCT                                                                                >  SRR4302535.2988904/1‑94 (MQ=60)
               CACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCCGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGTCT                                                                                >  SRR4302535.3656111/1‑94 (MQ=60)
                ACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTA                                                                               >  SRR4302535.3149075/1‑94 (MQ=60)
                  CAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAAAAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAAC                                                                             >  SRR4302535.2270846/1‑94 (MQ=60)
                   AGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTTCTGGAGCCTAACC                                                                            >  SRR4302535.2737320/1‑94 (MQ=60)
                   AGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACC                                                                            >  SRR4302535.2737321/1‑94 (MQ=60)
                   AGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACC                                                                            <  SRR4302535.526267/94‑1 (MQ=60)
                    GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302535.1456369/94‑1 (MQ=60)
                    GTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCA                                                                           <  SRR4302535.3095319/94‑1 (MQ=60)
                      ACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCCCACGACATTACTGGAGCCTAACCAGG                                                                         <  SRR4302535.3124090/94‑1 (MQ=60)
                       CGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGAGCTCACGACATTACTGGAGCCTAACCAGGC                                                                        >  SRR4302535.3493714/1‑94 (MQ=60)
                       CGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACCAGAGCGCCAACAGACCCAGCAAGGCGCTCACCACATTACTGGAGCCTAACCAGGC                                                                        >  SRR4302535.914826/1‑94 (MQ=60)
                       CGCAAAACATGGCGGGCGTCATCCCTTCCCCGACACACGAGAGCGCCAACAGCCCCAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGC                                                                        <  SRR4302535.8023/94‑1 (MQ=60)
                        GCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCAAGACATTACTGGGGCCTAACCAGGCG                                                                       >  SRR4302535.4143421/1‑94 (MQ=60)
                           AAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGAGCTCACGACATTACTGGAGCCTAACCAGGCGCTA                                                                    >  SRR4302535.3584877/1‑94 (MQ=60)
                           AAACAT‑GCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCCAT                                                                   >  SRR4302535.4317202/1‑94 (MQ=60)
                           AAACAGGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTA                                                                    <  SRR4302535.139298/94‑1 (MQ=60)
                            acaATGGCGGGCGTCATCCCTTTCCCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAT                                                                   <  SRR4302535.3640861/91‑1 (MQ=60)
                            AACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTAT                                                                   <  SRR4302535.3677846/94‑1 (MQ=60)
                             ACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATT                                                                  >  SRR4302535.1885705/1‑94 (MQ=60)
                              CGTGGCGGGCGCCATCCTTTTACCGGCACACGAGAGCGCAAACAGCCCCAGCAGGGCGCCCACGACATTACTGGAGCCTAACCAGGCGCTATTG                                                                 <  SRR4302535.3352351/94‑1 (MQ=60)
                              CAGGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTG                                                                 <  SRR4302535.4531876/94‑1 (MQ=60)
                               ATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGC                                                                <  SRR4302535.1335492/94‑1 (MQ=60)
                                  GCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAA                                                             <  SRR4302535.1466025/94‑1 (MQ=60)
                                   CGGGGGTCATCCCTTTACCGGCACACGAGAGCCCCAACAGCCCCAGCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAAT                                                            <  SRR4302535.1223348/94‑1 (MQ=60)
                                    GGGCGTCACCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATA                                                           <  SRR4302535.1958841/94‑1 (MQ=60)
                                     GGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAA                                                          >  SRR4302535.1308698/1‑94 (MQ=60)
                                      GCGTCATCCCTTTACGGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAAT                                                         <  SRR4302535.1634178/94‑1 (MQ=60)
                                       cctcccccctacaccgacacaggagcgcccaaacaccCCCAGCAAGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302535.3234671/57‑1 (MQ=60)
                                       CGTCATCCCTTTCCCGGCACACGAGAGCGCAAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302535.4161526/94‑1 (MQ=60)
                                       CGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        <  SRR4302535.666861/94‑1 (MQ=60)
                                       CGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATG                                                        >  SRR4302535.1067312/1‑94 (MQ=60)
                                        GTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       >  SRR4302535.2066997/1‑94 (MQ=60)
                                        GTCACCCCTTTCCCGCCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGG                                                       <  SRR4302535.145551/94‑1 (MQ=60)
                                          CATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302535.1028832/94‑1 (MQ=60)
                                          CATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     >  SRR4302535.1351995/1‑94 (MQ=60)
                                          CATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     >  SRR4302535.3766682/1‑94 (MQ=60)
                                          CATCCCTTCACCGGCACACGAGAGCGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTG                                                     <  SRR4302535.4429215/94‑1 (MQ=60)
                                            TCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCG                                                   <  SRR4302535.3179968/94‑1 (MQ=60)
                                             CCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGA                                                  <  SRR4302535.2342225/94‑1 (MQ=60)
                                               CTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAA                                                >  SRR4302535.1941069/1‑94 (MQ=60)
                                                tttcccggaccagGAGAGCGCCAACAGACCCAGCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAAC                                               <  SRR4302535.1748191/81‑1 (MQ=60)
                                                 TTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACG                                              <  SRR4302535.3791311/94‑1 (MQ=60)
                                                 TTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACG                                              <  SRR4302535.811800/94‑1 (MQ=60)
                                                  TACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGC                                             >  SRR4302535.1494019/1‑94 (MQ=60)
                                                   ACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCG                                            <  SRR4302535.3842966/94‑1 (MQ=60)
                                                    CCGCCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGC                                           <  SRR4302535.4422875/94‑1 (MQ=60)
                                                      GGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCA                                         <  SRR4302535.2929394/94‑1 (MQ=60)
                                                       GCACACGAGAGCGCCAACAGACCCAGCAAGCCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCAT                                        <  SRR4302535.4654917/94‑1 (MQ=60)
                                                        CACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATC                                       >  SRR4302535.135858/1‑94 (MQ=60)
                                                         ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      >  SRR4302535.877799/1‑94 (MQ=60)
                                                         ACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCT                                      <  SRR4302535.2870576/94‑1 (MQ=60)
                                                          caagagacccCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTG                                     <  SRR4302535.4109273/84‑1 (MQ=60)
                                                           ACGAGAGCGACAACACCCCCACCAGGGCCCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGC                                    <  SRR4302535.2548743/94‑1 (MQ=60)
                                                               GAGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAAC                                >  SRR4302535.2327054/1‑94 (MQ=60)
                                                                AGCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACC                               <  SRR4302535.1389310/94‑1 (MQ=60)
                                                                 GCGCCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCA                              <  SRR4302535.1471725/94‑1 (MQ=60)
                                                                  CGCCAACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAA                             <  SRR4302535.3034559/94‑1 (MQ=60)
                                                                    CCAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATG                           >  SRR4302535.2683408/1‑94 (MQ=60)
                                                                     CAACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGT                          <  SRR4302535.1139806/94‑1 (MQ=60)
                                                                       ACAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        <  SRR4302535.1860659/94‑1 (MQ=60)
                                                                       ACAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTT                        >  SRR4302535.3159674/1‑94 (MQ=60)
                                                                        CAGCCCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       <  SRR4302535.157114/94‑1 (MQ=60)
                                                                        CAGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTG                       <  SRR4302535.2099363/94‑1 (MQ=60)
                                                                         AGACCCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGA                      >  SRR4302535.2028870/1‑94 (MQ=60)
                                                                             CCAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTT                  <  SRR4302535.2004826/94‑1 (MQ=60)
                                                                              CAGCAGGGCGCTCACGACATTACTGGAGCCTAACCAGGCACTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 <  SRR4302535.2553426/94‑1 (MQ=60)
                                                                              CAGCAGGGCCCCCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 <  SRR4302535.3213578/94‑1 (MQ=60)
                                                                              CAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 >  SRR4302535.4382490/1‑94 (MQ=60)
                                                                              CAGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTA                 >  SRR4302535.4444815/1‑94 (MQ=60)
                                                                               AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                <  SRR4302535.3621417/94‑1 (MQ=60)
                                                                               AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302535.794246/1‑94 (MQ=60)
                                                                               AGCAAGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAA                >  SRR4302535.4417430/1‑94 (MQ=60)
                                                                                   AGGCGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACC            <  SRR4302535.3725008/94‑1 (MQ=60)
                                                                                    GGCTCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCC           <  SRR4302535.3234751/94‑1 (MQ=60)
                                                                                      CGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGC         >  SRR4302535.1709371/1‑94 (MQ=60)
                                                                                      CGCTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGC         >  SRR4302535.1029828/1‑94 (MQ=60)
                                                                                        CTCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCA       >  SRR4302535.77399/1‑94 (MQ=60)
                                                                                         TCACGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAA      <  SRR4302535.1340824/94‑1 (MQ=60)
                                                                                            CGACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTCCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTC   >  SRR4302535.3281190/1‑94 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  <  SRR4302535.3496375/94‑1 (MQ=60)
                                                                                             GACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  <  SRR4302535.2802317/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTACGATTGCACAATCACCAGTACGCCAAACATGGCGGGCGTCATCCCTTTACCGGCACACGAGAGCGCCAACAGACCCAGCAAGGCGCTCACAACATTACTGGAGCCTAACCAGGCGCTATTGCGAATAATGGTGCGAAACGCGCCATCTGCAAACCAATGTTTGATGTTAAAACCCGCCAATTCA  >  NC_000913/3130241‑3130427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑