Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,208,639 T→C intergenic (+2/‑109) rpsU → / → dnaG 30S ribosomal subunit protein S21/DNA primase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,208,6390TC99.4% 529.1 / ‑5.0 175intergenic (+2/‑109)rpsU/dnaG30S ribosomal subunit protein S21/DNA primase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (66/107);  total (68/107)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.31e-01

Only 100 of 212 total aligned reads displayed.

CCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAA  >  NC_000913/3208549‑3208728
                                                                                          |                                                                                         
ccGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATccc                                                                                         >  1:4504905/1‑93 (MQ=255)
ccGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATccc                                                                                         <  1:4106677/93‑1 (MQ=255)
ccGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAAAGCACGCCGCACTCGCCTGTACTAATccc                                                                                         >  1:3545231/1‑93 (MQ=255)
    aCGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTCCTAATCCCCCga                                                                                     >  1:1214406/1‑93 (MQ=255)
      gTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCCCGCCGCACTCGTCTGTACTAATACCCCgcga                                                                                   >  2:705666/1‑93 (MQ=255)
      gTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCgaga                                                                                   <  1:2883031/93‑1 (MQ=255)
      gTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCgaga                                                                                   <  2:1178612/93‑1 (MQ=255)
       tAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGagag                                                                                  <  2:2942542/93‑1 (MQ=255)
       tAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGagag                                                                                  >  1:4238448/1‑93 (MQ=255)
         aGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGcgcg                                                                                >  2:1104368/1‑93 (MQ=255)
           cgcgcTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGtt                                                                              <  2:2188224/93‑1 (MQ=255)
               cTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATACCCCGCGCGCGGTGctc                                                                          >  2:908018/1‑93 (MQ=255)
               cTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGACTGTACTAATCCCCCGAGAACGTTGccc                                                                          >  1:1202876/1‑91 (MQ=255)
                tAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGctct                                                                         >  2:3923165/1‑93 (MQ=255)
                tAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGctct                                                                         <  2:1553726/93‑1 (MQ=255)
                tAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCCGTGctct                                                                         >  2:4072967/1‑93 (MQ=255)
                 aaaGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCtctc                                                                        <  1:680167/93‑1 (MQ=255)
                 aaaGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCtctc                                                                        <  2:220201/93‑1 (MQ=255)
                 aaaGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAAAGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCtctc                                                                        >  1:3920163/1‑93 (MQ=255)
                  aaGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGGAAACGAACCCCGCAATCGCCTGGACTAATCCCCCGAGAGCGGTGGTcccc                                                                       >  1:2968559/1‑93 (MQ=255)
                   aGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCg                                                                      <  2:4383116/93‑1 (MQ=255)
                    gCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGa                                                                     >  1:1444282/1‑93 (MQ=255)
                     cTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGAt                                                                    <  2:1373650/93‑1 (MQ=255)
                      ttCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATc                                                                   <  1:2911322/93‑1 (MQ=255)
                       tCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCa                                                                  >  2:2834441/1‑93 (MQ=255)
                          gCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGGTCTCCGATCAGAc                                                               >  1:1063255/1‑93 (MQ=255)
                          gCAGTGAAACGTCACGCGAAGAAACTGGCTCCCGAAAAAGCAAACCCCCCTCCTTTGTCCTAATCCCCCGAAACGGCTGGCCTCCGATCAGAc                                                               >  1:1615411/1‑93 (MQ=255)
                          gCAGTGAAACCTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAACCCCCCGAGAGCGTTTCTCTCCGATCAGAc                                                               <  2:3079379/93‑1 (MQ=255)
                           cAGTGAAACGTCACGCGAAGAAACTGGCTCCCGAAAACGCACGCCGCACTCGTGTGTACTAATCCCCCGAGAGCGTTGCTCTCCGCTCAGAcc                                                              >  1:1578076/1‑93 (MQ=255)
                             gTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGGTGCTCTCCGGCCAGAcccc                                                            >  1:1727912/1‑91 (MQ=255)
                             gTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAAAGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGa                                                            >  1:1804882/1‑93 (MQ=255)
                               gAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGCTCAGACCGagt                                                          >  1:4361744/1‑93 (MQ=255)
                               gAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGagt                                                          >  1:1705626/1‑93 (MQ=255)
                               gAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGagt                                                          <  2:756560/93‑1 (MQ=255)
                                aaaCGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGagtt                                                         <  1:604067/93‑1 (MQ=255)
                                  aCGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCCATCAGACCGagttgt                                                       >  1:3212512/1‑93 (MQ=255)
                                     tCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGagttgtagt                                                    >  1:666143/1‑93 (MQ=255)
                                      cACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGagttgtagtt                                                   <  2:3583577/93‑1 (MQ=255)
                                       aCGCGAAGAAACTGGCTCGCGAAAACGGAAGCCGCACTCGGCTGTACTAATTCCCCGCGAGCGTTGCTATCCGATCAGACCGagttgtagttt                                                  >  2:1448248/1‑92 (MQ=255)
                                       aCGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGagttgtagttg                                                  >  1:1161739/1‑93 (MQ=255)
                                       aCGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGGTGCTCTCCGATCAGACCGagttgtagttg                                                  >  1:3532002/1‑93 (MQ=255)
                                       aCGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCACCCGAGAGCGGTGCTCTCCGCTCAGACCCagttgtagttg                                                  >  1:649850/1‑93 (MQ=255)
                                        cgcgAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGagttgtagttgt                                                 <  2:2705792/93‑1 (MQ=255)
                                         gcgAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAgttgtagttgta                                                <  2:917775/93‑1 (MQ=255)
                                          cgAAGACACTGGCTCGCGAAACCGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTaa                                               <  2:1870543/93‑1 (MQ=255)
                                          cgAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTaa                                               <  2:4451139/93‑1 (MQ=255)
                                           gaagaaACTGGCTCGCGAAAAAGCACGCCGCACTCGTCTGTACCAATCCCCCGAGAACGTTGGTCTCCGATCAGATCGAGGTGTGGTTGTAAg                                              >  2:3439533/1‑93 (MQ=255)
                                            aagaaACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAgg                                             >  1:807621/1‑93 (MQ=255)
                                            aagaaACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAgg                                             >  1:3435225/1‑93 (MQ=255)
                                            aagaaACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAgg                                             <  1:2602282/93‑1 (MQ=255)
                                                aaCTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGt                                         <  2:3331308/93‑1 (MQ=255)
                                                  cTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGc                                       <  2:3147398/93‑1 (MQ=255)
                                                   tGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGGTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCt                                      >  1:2443329/1‑93 (MQ=255)
                                                     gCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTc                                    <  2:4538741/93‑1 (MQ=255)
                                                     gCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTc                                    <  2:1606424/93‑1 (MQ=255)
                                                      cTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTcc                                   <  2:4651595/93‑1 (MQ=255)
                                                      cTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTcc                                   <  1:3050240/93‑1 (MQ=255)
                                                      cTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTcc                                   <  1:1493987/93‑1 (MQ=255)
                                                          cgAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGaaa                               >  1:4575751/1‑93 (MQ=255)
                                                           gAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAg                              <  2:1142001/93‑1 (MQ=255)
                                                           gAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAg                              >  2:107424/1‑93 (MQ=255)
                                                           gAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAg                              <  1:3577357/93‑1 (MQ=255)
                                                           gAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAg                              >  1:2377845/1‑93 (MQ=255)
                                                           gAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAg                              <  2:1218753/93‑1 (MQ=255)
                                                            aaaaCGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAgg                             <  2:2537456/93‑1 (MQ=255)
                                                            aaaaCGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAgg                             <  1:2253214/93‑1 (MQ=255)
                                                            aaaaCGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAgg                             >  1:2166705/1‑93 (MQ=255)
                                                            aaaaCGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAgg                             >  1:4182902/1‑93 (MQ=255)
                                                             aaaCGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGa                            <  1:2145884/93‑1 (MQ=255)
                                                             aaaCGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGa                            >  2:2883754/1‑93 (MQ=255)
                                                              aaCGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGaa                           >  1:3337335/1‑93 (MQ=255)
                                                              aaCGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGaa                           <  2:548258/93‑1 (MQ=255)
                                                              aaCGCACGCCGCACTCGTATGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGaa                           <  2:2282148/93‑1 (MQ=255)
                                                                cgcacgcCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATg                         <  2:42338/93‑1 (MQ=255)
                                                                 gcacgcCTCACTCGTCTGTACTAACCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATgc                        <  1:1699324/93‑1 (MQ=255)
                                                                 gcacgcCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATgc                        <  2:451356/93‑1 (MQ=255)
                                                                   acgcCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATgcgc                      <  1:2648395/93‑1 (MQ=255)
                                                                   acgcCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATgcgc                      <  2:3207964/93‑1 (MQ=255)
                                                                   acgcCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATgcgc                      <  2:3389272/93‑1 (MQ=255)
                                                                    cgccgcACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGcgcg                     <  2:2387363/93‑1 (MQ=255)
                                                                       cgcACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCt                  <  2:4257572/93‑1 (MQ=255)
                                                                        ccACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCtt                 <  1:4302412/92‑1 (MQ=255)
                                                                         cACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTa                <  2:4141733/93‑1 (MQ=255)
                                                                          aCTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAt               >  1:2406263/1‑93 (MQ=255)
                                                                          aCTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAt               >  1:1659391/1‑93 (MQ=255)
                                                                           cTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAtt              <  2:4104505/93‑1 (MQ=255)
                                                                           cTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAtt              >  1:2832898/1‑93 (MQ=255)
                                                                            tCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAttt             <  1:208424/93‑1 (MQ=255)
                                                                            tCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAttt             <  2:2086233/93‑1 (MQ=255)
                                                                              gTCTGTACTAATACCCCGATAGCGTTTCTCTCCGATCAGACCGTGTTGTAGTTTTAAGGTCGTGCTTTCGAGCGGAATGCGCGGCTTTTTTTc           >  2:3992636/1‑93 (MQ=255)
                                                                               tCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCg          >  2:2311504/1‑93 (MQ=255)
                                                                                 tGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGtt        >  1:952021/1‑93 (MQ=255)
                                                                                 tGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGtt        >  2:2056640/1‑93 (MQ=255)
                                                                                  gTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGttt       >  2:3892970/1‑93 (MQ=255)
                                                                                   tACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTa      <  2:151914/93‑1 (MQ=255)
                                                                                   tACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTa      <  2:1735347/93‑1 (MQ=255)
                                                                                      tAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGa   >  2:459079/1‑93 (MQ=255)
                                                                                      tAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGa   >  1:1840623/1‑93 (MQ=255)
                                                                                      tAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGa   <  1:522182/93‑1 (MQ=255)
                                                                                       aaTCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGaa  <  2:3821275/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAA  >  NC_000913/3208549‑3208728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAATTG  >  NC_000913/3208546‑3208731
                                                                                             |                                                                                            
CTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATC                                                                                              <  SRR4302535.979970/94‑1 (MQ=60)
 TACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCC                                                                                             <  SRR4302535.2622965/94‑1 (MQ=60)
 TACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCC                                                                                             <  SRR4302535.1495419/94‑1 (MQ=60)
 TACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCCTCTGTAATAATCC                                                                                             >  SRR4302535.4022083/1‑94 (MQ=60)
 TACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCCCGAAAACGCACGCCGCACTCGTCTGTACTAATCC                                                                                             >  SRR4302535.2818401/1‑94 (MQ=60)
  ACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCC                                                                                            <  SRR4302535.3943825/94‑1 (MQ=60)
  ACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCC                                                                                            >  SRR4302535.4535835/1‑94 (MQ=60)
  ACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCC                                                                                            >  SRR4302535.3087833/1‑94 (MQ=60)
  ACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAAAGCACGCCGCACTCGCCTGTACTAATCCC                                                                                            >  SRR4302535.3569849/1‑94 (MQ=60)
   CCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCC                                                                                           <  SRR4302535.4134796/94‑1 (MQ=60)
      AACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTCCTAATCCCCCGA                                                                                        >  SRR4302535.1223113/1‑94 (MQ=60)
        CGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCCAGA                                                                                      >  SRR4302535.4014673/1‑94 (MQ=60)
         GTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAG                                                                                     <  SRR4302535.1674003/94‑1 (MQ=60)
         GTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAG                                                                                     >  SRR4302535.4267466/1‑94 (MQ=60)
         GTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAG                                                                                     <  SRR4302535.2903436/94‑1 (MQ=60)
           AAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCG                                                                                   <  SRR4302535.2770882/94‑1 (MQ=60)
            AGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGgct                                                                                  >  SRR4302535.2441466/1‑91 (MQ=60)
             GCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTT                                                                                 <  SRR4302535.3767253/94‑1 (MQ=60)
              CGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCCCGAAAAAGCACGCCGCACTCCGCTGTACTCATTCCCCAAGAGCGTCG                                                                                >  SRR4302535.1439855/1‑94 (MQ=60)
                CGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCT                                                                              <  SRR4302535.4449891/94‑1 (MQ=60)
                CGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCT                                                                              <  SRR4302535.676225/94‑1 (MQ=60)
                 GCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGACTGTACTAATCCCCCGAGAACGTTGCCC                                                                             >  SRR4302535.1211511/1‑94 (MQ=60)
                   TAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAAAGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTC                                                                           >  SRR4302535.3946970/1‑94 (MQ=60)
                    AAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGGAAACGAACCCCGCAATCGCCTGGACTAATCCCCCGAGAGCGgtggtcccc                                                                          >  SRR4302535.2989390/1‑85 (MQ=60)
                    AAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCC                                                                          <  SRR4302535.1641021/94‑1 (MQ=60)
                    AAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCC                                                                          <  SRR4302535.684933/94‑1 (MQ=60)
                    AAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCC                                                                          <  SRR4302535.119203/94‑1 (MQ=60)
                      AGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGA                                                                        >  SRR4302535.1454610/1‑94 (MQ=60)
                        CTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATC                                                                      <  SRR4302535.781901/94‑1 (MQ=60)
                         TTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCA                                                                     <  SRR4302535.2931885/94‑1 (MQ=60)
                            TGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGGTCTCCGATCAGAC                                                                  >  SRR4302535.1070823/1‑94 (MQ=60)
                             GCAGTGAAACGTCACGCGAAGAAACTGGCTCCCGAAAACGCACGCCGCACTCGTGTGTACTAATCCCCCGAGAGCGTTGCTCTCCGCTCAGACC                                                                 >  SRR4302535.1589464/1‑94 (MQ=60)
                               AGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGGTGCTCTCCGgccagacccc                                                               >  SRR4302535.1740401/1‑84 (MQ=60)
                               AGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAAAGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGA                                                               >  SRR4302535.1817926/1‑94 (MQ=60)
                               AGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAAAGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCCGTGCTCTCCGATCAGACCGA                                                               >  SRR4302535.1739103/1‑94 (MQ=60)
                                 TGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGGACTCGTCTGTACTAATCCCCCGAGAGCGTTAGTCTCCGATCAGACCGAGT                                                             >  SRR4302535.2891220/1‑94 (MQ=60)
                                 TGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGCGAGCGTTGCTCTCCGATCCGACCCAGT                                                             >  SRR4302535.1682104/1‑94 (MQ=60)
                                 TGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGCTCAGACCGAGT                                                             >  SRR4302535.4391594/1‑94 (MQ=60)
                                 TGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGT                                                             >  SRR4302535.1717958/1‑94 (MQ=60)
                                  GAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTT                                                            <  SRR4302535.171551/94‑1 (MQ=60)
                                   AAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGCGAGCGCTGCTCTCCGATCAGACCGAGTTT                                                           >  SRR4302535.3266279/1‑94 (MQ=60)
                                   AAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTG                                                           >  SRR4302535.66964/1‑94 (MQ=60)
                                   AAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTG                                                           <  SRR4302535.608295/94‑1 (MQ=60)
                                   AAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTG                                                           <  SRR4302535.1764929/94‑1 (MQ=60)
                                    AACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGGGTTGCTCTCCGATCAGACCGAGTTGT                                                          >  SRR4302535.4248516/1‑94 (MQ=60)
                                    AACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCCATCAGACCGAGTTGT                                                          >  SRR4302535.3234760/1‑94 (MQ=60)
                                      CGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAG                                                        <  SRR4302535.462127/94‑1 (MQ=60)
                                      CGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAG                                                        >  SRR4302535.3582966/1‑94 (MQ=60)
                                       GTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGT                                                       >  SRR4302535.670789/1‑94 (MQ=60)
                                        TCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTT                                                      <  SRR4302535.929328/94‑1 (MQ=60)
                                        TCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTT                                                      <  SRR4302535.3400237/94‑1 (MQ=60)
                                         CACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTG                                                     >  SRR4302535.1170043/1‑94 (MQ=60)
                                         CACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTG                                                     <  SRR4302535.2401221/94‑1 (MQ=60)
                                         CACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGGTGCTCTCCGATCAGACCGAGTTGTAGTTG                                                     >  SRR4302535.3556523/1‑94 (MQ=60)
                                         CACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCACCCGAGAGCGGTGCTCTCCGCTCAGACCCAGTTGTAGTTG                                                     >  SRR4302535.654382/1‑94 (MQ=60)
                                             CGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAG                                                 <  SRR4302535.3760759/94‑1 (MQ=60)
                                             CGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAG                                                 <  SRR4302535.4353114/94‑1 (MQ=60)
                                              GAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGG                                                >  SRR4302535.3459098/1‑94 (MQ=60)
                                              GAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGG                                                >  SRR4302535.813281/1‑94 (MQ=60)
                                              GAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGG                                                <  SRR4302535.2243782/94‑1 (MQ=60)
                                               AAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGC                                               <  SRR4302535.2620964/94‑1 (MQ=60)
                                                AGAAACTGGCCCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCC                                              <  SRR4302535.3566860/94‑1 (MQ=60)
                                                     ctggctgcgGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCT                                         <  SRR4302535.118531/85‑1 (MQ=60)
                                                     CTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCT                                         <  SRR4302535.2989111/94‑1 (MQ=60)
                                                     CTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGGTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCT                                         >  SRR4302535.2460932/1‑94 (MQ=60)
                                                      TGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTT                                        <  SRR4302535.3539775/94‑1 (MQ=60)
                                                      TGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTT                                        <  SRR4302535.1836755/94‑1 (MQ=60)
                                                        GCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCC                                      <  SRR4302535.1135579/94‑1 (MQ=60)
                                                         CTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCG                                     <  SRR4302535.3071605/94‑1 (MQ=60)
                                                         CTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCG                                     <  SRR4302535.1504725/94‑1 (MQ=60)
                                                          TCGCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGA                                    <  SRR4302535.4594636/94‑1 (MQ=60)
                                                            GCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAA                                  >  SRR4302535.4606932/1‑94 (MQ=60)
                                                            GCGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAA                                  <  SRR4302535.1517417/94‑1 (MQ=60)
                                                             CGAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAG                                 >  SRR4302535.2394924/1‑94 (MQ=60)
                                                              GAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGG                                <  SRR4302535.2025953/94‑1 (MQ=60)
                                                              GAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGG                                >  SRR4302535.2182237/1‑94 (MQ=60)
                                                              GAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGG                                >  SRR4302535.4211523/1‑94 (MQ=60)
                                                              GAAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGG                                <  SRR4302535.3602157/94‑1 (MQ=60)
                                                               AAAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGA                               <  SRR4302535.2269262/94‑1 (MQ=60)
                                                                AAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAA                              >  SRR4302535.3360581/1‑94 (MQ=60)
                                                                AAACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAA                              <  SRR4302535.2161322/94‑1 (MQ=60)
                                                                 AACGCACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAAT                             <  SRR4302535.2222288/94‑1 (MQ=60)
                                                                    GCACGCCTCACTCGTCTGTACTAACCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCG                          <  SRR4302535.1711604/94‑1 (MQ=60)
                                                                     CACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGC                         >  SRR4302535.1215218/1‑94 (MQ=60)
                                                                     CACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGC                         >  SRR4302535.2893558/1‑94 (MQ=60)
                                                                     CACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGC                         >  SRR4302535.607542/1‑94 (MQ=60)
                                                                      ACGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCG                        <  SRR4302535.2667364/94‑1 (MQ=60)
                                                                       CGCCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGG                       >  SRR4302535.979605/1‑94 (MQ=60)
                                                                         CCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCT                     >  SRR4302535.2137521/1‑94 (MQ=60)
                                                                         CCGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCT                     <  SRR4302535.2923744/94‑1 (MQ=60)
                                                                          CGCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTT                    >  SRR4302535.4160724/1‑94 (MQ=60)
                                                                           CCACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTA                   <  SRR4302535.4331845/94‑1 (MQ=60)
                                                                            CACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAT                  >  SRR4302535.4225860/1‑94 (MQ=60)
                                                                            CACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAT                  >  SRR4302535.1671386/1‑94 (MQ=60)
                                                                            CACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAT                  >  SRR4302535.2423584/1‑94 (MQ=60)
                                                                            CACTCCTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTAT                  >  SRR4302535.1470661/1‑94 (MQ=60)
                                                                             ACTCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATT                 >  SRR4302535.2853007/1‑94 (MQ=60)
                                                                               TCGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTT               <  SRR4302535.209855/94‑1 (MQ=60)
                                                                                CGTCTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTC              <  SRR4302535.3052873/94‑1 (MQ=60)
                                                                                   CTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTT           >  SRR4302535.958770/1‑94 (MQ=60)
                                                                                   CTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTT           <  SRR4302535.629285/94‑1 (MQ=60)
                                                                                   CTGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGCTGTAGTTGTAAGGCCGTGCTTGCGAAAGGAATGCGCGGCTTATTTTCGTT           >  SRR4302535.2465332/1‑94 (MQ=60)
                                                                                    TGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCGGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTT          >  SRR4302535.2181742/1‑94 (MQ=60)
                                                                                    TGTACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTT          >  SRR4302535.2351121/1‑94 (MQ=60)
                                                                                      GACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTAT        <  SRR4302535.473011/94‑1 (MQ=60)
                                                                                       ACTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATG       <  SRR4302535.4299219/94‑1 (MQ=60)
                                                                                        CTAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGA      >  SRR4302535.1853900/1‑94 (MQ=60)
                                                                                         TAATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAA     <  SRR4302535.525834/94‑1 (MQ=60)
                                                                                          AATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAAT    >  SRR4302535.580895/1‑94 (MQ=60)
                                                                                          AATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAAT    <  SRR4302535.159148/94‑1 (MQ=60)
                                                                                           ATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAATT   <  SRR4302535.3568437/94‑1 (MQ=60)
                                                                                           ATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAATT   >  SRR4302535.580/1‑94 (MQ=60)
                                                                                           ATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAATT   >  SRR4302535.3924375/1‑94 (MQ=60)
                                                                                           ATCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAATT   >  SRR4302535.1229393/1‑94 (MQ=60)
                                                                                            TCCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAATTG  >  SRR4302535.2717391/1‑94 (MQ=60)
                                                                                             |                                                                                            
CTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCGCGAAAACGCACGCCGCACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAATTG  >  NC_000913/3208546‑3208731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑